Rh4AG135600

Bacterial trigger factor protein (TF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0011553
Physical Location & Seq
Reverse (-)
29776551 .. 29780064
3514 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG135600.1

Sequence Viewer

Length: 672 bp
ATGGCATCGTTGACGACGATGATAACAGTTCCGTCGAATTTCCCCTCCCTTGGACTTCCCATGCACTTTTCTGTGAGAAAACACTGCACTTGTGTCGTTGCTTATCAAAATGCCGCCACAAAAATGGGGTCATATAGCTCTCAACATAATCTTTTCACTCGTGTGCATGAGATTGGTATTGCACGCTTATCCAGGACGATATTCGCAGCCGGTTCAGGTTTAGAGGCATCCATTGCGGAAGAAAATGTGATAAAGTTGAAAACCACAAAGATTGTTGTCGAGTCCCAAGATGAGAATAGTATACAAGTTAGAGTGGATGTGGCTGGGGATGAAACGCAAAAAGTTTTTGATCAGGTTTTGACAAATTTGGCACGCACAGCTCCACCAGTTCCAGGATTTCGCAGACAAAAAGGAGGAAAAACATCAAAGGTTCCAAAAAGCTTTCTCTTGGATATACTTGGCAAAGAGCGTGTCACAAAGTTTGTCATACAAGAATTAGTAAGCTCTGCTATGGCTGATTATGTAAAGCAGGAAAATCTGACTGTGAAGGAAAACAAGATCAACACCACCCAATCGGCAGAAGAACTCCAATCGTTGTTCACTCCGGGAAATGAATTTGCATTTAATGCTATAGTTGAACTTGAAAAGTCAGAAATTGAAAAGCCAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Trigger_N

Protein Analysis

223

Amino Acids

24.56

Weight (kDa)

8.48

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trigger_N PF05697 90 - 214 4.9e-13 Bacterial trigger factor protein (TF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 301
AciI CCGC 2 cut(s) 114, 236
AcsI RAATTY 3 cut(s) 37, 364, 614
AfiI CCNNNNNNNGG 2 cut(s) 50, 392
AgsI TTSAA 4 cut(s) 259, 638, 644, 659
AjnI CCWGG 2 cut(s) 191, 391
AleI CACNNNNGTG 1 cut(s) 161
AluBI AGCT 4 cut(s) 138, 380, 441, 504
AluI AGCT 4 cut(s) 138, 380, 441, 504
ApeKI GCWGC 1 cut(s) 206
ApoI RAATTY 3 cut(s) 37, 364, 614
ArsI GACNNNNNNTTYG 4 cut(s) 113, 145, 456, 488
AsuC2I CCSGG 1 cut(s) 606
BauI CACGAG 1 cut(s) 159
BbvI GCAGC 1 cut(s) 218
BcgI CGANNNNNNTGC 2 cut(s) 76, 110
BciT130I CCWGG 2 cut(s) 193, 393
BclI TGATCA 1 cut(s) 349
BcnI CCSGG 1 cut(s) 606
BfmI CTRYAG 1 cut(s) 630
BisI GCNGC 2 cut(s) 114, 207
BlsI GCNGC 2 cut(s) 115, 208
Bme1390I CCNGG 3 cut(s) 193, 393, 606
BmiI GGNNCC 1 cut(s) 432
BmrFI CCNGG 3 cut(s) 193, 393, 606
BmsI GCATC 2 cut(s) 14, 236
BpuMI CCSGG 1 cut(s) 606
BsaJI CCNNGG 1 cut(s) 49
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 392
Bse118I RCCGGY 1 cut(s) 209
Bse1I ACTGG 1 cut(s) 386
Bse3DI GCAATG 1 cut(s) 231
BseBI CCWGG 2 cut(s) 193, 393
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 3 cut(s) 227, 322, 334
BseLI CCNNNNNNNGG 2 cut(s) 50, 392
BseMI GCAATG 1 cut(s) 231
BseNI ACTGG 1 cut(s) 386
BseXI GCAGC 1 cut(s) 218
BseYI CCCAGC 1 cut(s) 323
BsgI GTGCAG 1 cut(s) 70
BsiSI CCGG 2 cut(s) 210, 605
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 2 cut(s) 50, 392
BsmFI GGGAC 1 cut(s) 268
Bsp143I GATC 2 cut(s) 349, 558
BspACI CCGC 2 cut(s) 114, 236
BspLI GGNNCC 1 cut(s) 432
BsrDI GCAATG 1 cut(s) 231
BsrFI RCCGGY 1 cut(s) 209
BsrI ACTGG 1 cut(s) 386
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 2 cut(s) 349, 558
BssNAI GTATAC 1 cut(s) 302
BssSI CACGAG 1 cut(s) 159
BssT1I CCWWGG 1 cut(s) 49
Bst1107I GTATAC 1 cut(s) 302
Bst2BI CACGAG 1 cut(s) 159
Bst2UI CCWGG 2 cut(s) 193, 393
Bst4CI ACNGT 2 cut(s) 28, 544
BstAPI GCANNNNNTGC 2 cut(s) 233, 626
BstC8I GCNNGC 2 cut(s) 184, 373
BstF5I GGATG 3 cut(s) 227, 322, 334
BstKTI GATC 2 cut(s) 352, 561
BstMBI GATC 2 cut(s) 349, 558
BstMWI GCNNNNNNNGC 3 cut(s) 233, 377, 626
BstNI CCWGG 2 cut(s) 193, 393
BstSCI CCNGG 3 cut(s) 191, 391, 604
BstSFI CTRYAG 1 cut(s) 630
BstV1I GCAGC 1 cut(s) 218
BstXI CCANNNNNNTGG 1 cut(s) 124
BstZ17I GTATAC 1 cut(s) 302
BtsCI GGATG 3 cut(s) 227, 322, 334
BtsI GCAGTG 1 cut(s) 82
BtsIMutI CAGTG 1 cut(s) 82
Cac8I GCNNGC 2 cut(s) 184, 373
Cfr10I RCCGGY 1 cut(s) 209
CspCI CAANNNNNGTGG 2 cut(s) 253, 288
CviAII CATG 2 cut(s) 61, 167
CviJI RGCY 8 cut(s) 138, 209, 323, 380, 441, 504, 515, 664
CviKI_1 RGCY 8 cut(s) 138, 209, 323, 380, 441, 504, 515, 664
DpnI GATC 2 cut(s) 351, 560
DpnII GATC 2 cut(s) 349, 558
Eco130I CCWWGG 1 cut(s) 49
EcoRII CCWGG 2 cut(s) 191, 391
EcoT14I CCWWGG 1 cut(s) 49
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 2 cut(s) 64, 170
FaqI GGGAC 1 cut(s) 268
FatI CATG 2 cut(s) 60, 166
FbaI TGATCA 1 cut(s) 349
FblI GTMKAC 1 cut(s) 301
Fnu4HI GCNGC 2 cut(s) 114, 207
FokI GGATG 3 cut(s) 214, 329, 341
Fsp4HI GCNGC 2 cut(s) 114, 207
GluI GCNGC 2 cut(s) 114, 207
GsaI CCCAGC 1 cut(s) 327
HapII CCGG 2 cut(s) 210, 605
Hin1II CATG 2 cut(s) 64, 170
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HindIII AAGCTT 1 cut(s) 439
HinfI GANTC 1 cut(s) 281
HpaII CCGG 2 cut(s) 210, 605
Hpy166II GTNNAC 3 cut(s) 12, 302, 600
Hpy188I TCNGA 2 cut(s) 540, 652
Hpy8I GTNNAC 3 cut(s) 12, 302, 600
Hpy99I CGWCG 2 cut(s) 19, 37
HpyAV CCTTC 1 cut(s) 541
HpyCH4III ACNGT 2 cut(s) 28, 544
HpyCH4V TGCA 5 cut(s) 64, 87, 166, 182, 620
HpyF10VI GCNNNNNNNGC 3 cut(s) 233, 377, 626
Hsp92II CATG 2 cut(s) 64, 170
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 2 cut(s) 349, 558
LmnI GCTCC 1 cut(s) 385
Lsp1109I GCAGC 1 cut(s) 218
LweI GCATC 2 cut(s) 14, 236
MaeIII GTNAC 1 cut(s) 472
MalI GATC 2 cut(s) 351, 560
MboI GATC 2 cut(s) 349, 558
MboII GAAGA 2 cut(s) 251, 593
MluCI AATT 5 cut(s) 37, 364, 494, 614, 654
MlyI GAGTC 1 cut(s) 290
MnlI CCTC 3 cut(s) 55, 217, 407
MseI TTAA 1 cut(s) 624
MslI CAYNNNNRTG 2 cut(s) 122, 161
MspI CCGG 2 cut(s) 210, 605
MspR9I CCNGG 3 cut(s) 193, 393, 606
MvaI CCWGG 2 cut(s) 193, 393
MwoI GCNNNNNNNGC 3 cut(s) 233, 377, 626
NciI CCSGG 1 cut(s) 606
NdeII GATC 2 cut(s) 349, 558
NlaIII CATG 2 cut(s) 64, 170
NlaIV GGNNCC 1 cut(s) 432
NmuCI GTSAC 1 cut(s) 472
OliI CACNNNNGTG 1 cut(s) 161
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PfoI TCCNGGA 3 cut(s) 191, 391, 604
PkrI GCNGC 2 cut(s) 115, 208
PleI GAGTC 1 cut(s) 289
PpsI GAGTC 1 cut(s) 289
Psp6I CCWGG 2 cut(s) 191, 391
PspFI CCCAGC 1 cut(s) 323
PspGI CCWGG 2 cut(s) 191, 391
PspN4I GGNNCC 1 cut(s) 432
RseI CAYNNNNRTG 2 cut(s) 122, 161
SaqAI TTAA 1 cut(s) 624
SatI GCNGC 2 cut(s) 114, 207
Sau3AI GATC 2 cut(s) 349, 558
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 3 cut(s) 193, 393, 606
SetI ASST 8 cut(s) 140, 220, 357, 382, 432, 443, 506, 671
SfaNI GCATC 2 cut(s) 14, 236
SfcI CTRYAG 1 cut(s) 630
SmiMI CAYNNNNRTG 2 cut(s) 122, 161
Sse9I AATT 5 cut(s) 37, 364, 494, 614, 654
SsiI CCGC 2 cut(s) 114, 236
StyD4I CCNGG 3 cut(s) 191, 391, 604
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 2 cut(s) 28, 544
TaqI TCGA 2 cut(s) 35, 279
TasI AATT 5 cut(s) 37, 364, 494, 614, 654
TauI GCSGC 1 cut(s) 116
Tru1I TTAA 1 cut(s) 624
Tru9I TTAA 1 cut(s) 624
TscAI CASTG 1 cut(s) 89
TseFI GTSAC 1 cut(s) 472
TseI GCWGC 1 cut(s) 206
Tsp45I GTSAC 1 cut(s) 472
TspDTI ATGAA 2 cut(s) 345, 627
TspGWI ACGGA 1 cut(s) 21
TspRI CASTG 1 cut(s) 89
XapI RAATTY 3 cut(s) 37, 364, 614
XmiI GTMKAC 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.