RLG00000008799

Bacterial trigger factor protein (TF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0011553
Physical Location & Seq
Forward (+)
39395528 .. 39398797
3270 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008799

Sequence Viewer

Length: 672 bp
ATGGCGTCGTTGACAACGATGATAACAGTTCCGTCGAATTTCCCCTCCCTTGGACTTCCCATGCACTTTTCTGTGAGAAAACACTGCACTTGTGTCGTTGCTTGTCAAAATGCCGCCACAAAAATGGGGTCATATAGCTCTCAACATAATCTTTTCACTCGTGTGCATGAGATTGGTATTGCACGCTTACCCAGGCCGATATTCGCAGCCGGTTCAGGTTTAGAAGCATCCATTGCGGAAGAAAATGTGATAAAGTTGAAAACCACAAAGATTGTTGTCGAGTCCCAAGATGAGAATAGTATACAAGTTAGAGTGGACGTGGCTGGAGATGAAACGCAAAAAGTTTTTGATCAGGTTTTGACAAATTTGGCACGCACAGCTCCACCAGTTCCAGGATTTCGCAGACAAAAAGGAGGAAAAACATCAAAGGTTCCAAAAAGCTTTCTCTTGGATATACTTGGTAAAGAGCGCGTCACAAAGTTTGTCATACAAGAATTAGTAAGCTCTGCTATGGCTGATTATGTAAAGCAGGAAAATCTGACTGTGAAGGAAAACAAGATCAACACCACTCAATCGGCAGAAGAACTCCAATCGCTGTTCACTCCGGGGAATGAATTTGCATTTAATGCTATAGTTGAACTTGAAAAATCAGAAATTGAAAAGCCAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Trigger_N

Protein Analysis

224

Amino Acids

24.51

Weight (kDa)

8.39

Isoelectric Point (pI)

45.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trigger_N PF05697 90 - 214 4.9e-13 Bacterial trigger factor protein (TF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 301
AccII CGCG 1 cut(s) 471
AciI CCGC 2 cut(s) 114, 236
AcsI RAATTY 3 cut(s) 37, 364, 614
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 50, 392
AgsI TTSAA 4 cut(s) 259, 638, 644, 659
AjiI CACGTC 1 cut(s) 319
AjnI CCWGG 2 cut(s) 191, 391
AleI CACNNNNGTG 1 cut(s) 161
AluBI AGCT 4 cut(s) 138, 380, 441, 504
AluI AGCT 4 cut(s) 138, 380, 441, 504
AoxI GGCC 1 cut(s) 194
ApeKI GCWGC 1 cut(s) 206
ApoI RAATTY 3 cut(s) 37, 364, 614
ArsI GACNNNNNNTTYG 2 cut(s) 113, 145
AspLEI GCGC 1 cut(s) 471
AsuC2I CCSGG 1 cut(s) 606
BauI CACGAG 1 cut(s) 159
BbvI GCAGC 1 cut(s) 218
BcgI CGANNNNNNTGC 2 cut(s) 76, 110
BciT130I CCWGG 2 cut(s) 193, 393
BclI TGATCA 1 cut(s) 349
BcnI CCSGG 1 cut(s) 606
BfmI CTRYAG 1 cut(s) 630
BisI GCNGC 2 cut(s) 114, 207
BlsI GCNGC 2 cut(s) 115, 208
Bme1390I CCNGG 3 cut(s) 193, 393, 606
BmgBI CACGTC 1 cut(s) 319
BmiI GGNNCC 1 cut(s) 432
BmrFI CCNGG 3 cut(s) 193, 393, 606
BmsI GCATC 1 cut(s) 236
BpmI CTGGAG 1 cut(s) 345
BpuMI CCSGG 1 cut(s) 606
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 49, 191, 605
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 392
Bse118I RCCGGY 1 cut(s) 209
Bse1I ACTGG 1 cut(s) 386
Bse3DI GCAATG 1 cut(s) 231
BseBI CCWGG 2 cut(s) 193, 393
BseDI CCNNGG 3 cut(s) 49, 191, 605
BseGI GGATG 1 cut(s) 227
BseLI CCNNNNNNNGG 2 cut(s) 50, 392
BseMI GCAATG 1 cut(s) 231
BseNI ACTGG 1 cut(s) 386
BseXI GCAGC 1 cut(s) 218
BsgI GTGCAG 1 cut(s) 70
Bsh1236I CGCG 1 cut(s) 471
BshFI GGCC 1 cut(s) 196
BsiSI CCGG 2 cut(s) 210, 605
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 2 cut(s) 50, 392
BsmFI GGGAC 1 cut(s) 268
BsnI GGCC 1 cut(s) 196
Bsp143I GATC 2 cut(s) 349, 558
BspACI CCGC 2 cut(s) 114, 236
BspANI GGCC 1 cut(s) 196
BspFNI CGCG 1 cut(s) 471
BspLI GGNNCC 1 cut(s) 432
BsrDI GCAATG 1 cut(s) 231
BsrFI RCCGGY 1 cut(s) 209
BsrI ACTGG 1 cut(s) 386
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 3 cut(s) 49, 191, 605
BssMI GATC 2 cut(s) 349, 558
BssNAI GTATAC 1 cut(s) 302
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 159
BssT1I CCWWGG 1 cut(s) 49
Bst1107I GTATAC 1 cut(s) 302
Bst2BI CACGAG 1 cut(s) 159
Bst2UI CCWGG 2 cut(s) 193, 393
Bst4CI ACNGT 2 cut(s) 28, 544
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 2 cut(s) 233, 626
BstC8I GCNNGC 2 cut(s) 184, 373
BstF5I GGATG 1 cut(s) 227
BstFNI CGCG 1 cut(s) 471
BstHHI GCGC 1 cut(s) 471
BstKTI GATC 2 cut(s) 352, 561
BstMBI GATC 2 cut(s) 349, 558
BstMWI GCNNNNNNNGC 3 cut(s) 233, 377, 626
BstNI CCWGG 2 cut(s) 193, 393
BstSCI CCNGG 3 cut(s) 191, 391, 604
BstSFI CTRYAG 1 cut(s) 630
BstUI CGCG 1 cut(s) 471
BstV1I GCAGC 1 cut(s) 218
BstXI CCANNNNNNTGG 1 cut(s) 124
BstZ17I GTATAC 1 cut(s) 302
BsuRI GGCC 1 cut(s) 196
BtrI CACGTC 1 cut(s) 319
BtsCI GGATG 1 cut(s) 227
BtsI GCAGTG 1 cut(s) 82
BtsIMutI CAGTG 1 cut(s) 82
Cac8I GCNNGC 2 cut(s) 184, 373
CfoI GCGC 1 cut(s) 471
Cfr10I RCCGGY 1 cut(s) 209
CseI GACGC 1 cut(s) 460
CspCI CAANNNNNGTGG 2 cut(s) 253, 288
CviAII CATG 2 cut(s) 61, 167
CviJI RGCY 9 cut(s) 138, 196, 209, 323, 380, 441, 504, 515, 664
CviKI_1 RGCY 9 cut(s) 138, 196, 209, 323, 380, 441, 504, 515, 664
DpnI GATC 2 cut(s) 351, 560
DpnII GATC 2 cut(s) 349, 558
Eco130I CCWWGG 1 cut(s) 49
EcoRII CCWGG 2 cut(s) 191, 391
EcoT14I CCWWGG 1 cut(s) 49
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 2 cut(s) 64, 170
FaqI GGGAC 1 cut(s) 268
FatI CATG 2 cut(s) 60, 166
FbaI TGATCA 1 cut(s) 349
FblI GTMKAC 1 cut(s) 301
Fnu4HI GCNGC 2 cut(s) 114, 207
FokI GGATG 1 cut(s) 214
Fsp4HI GCNGC 2 cut(s) 114, 207
GlaI GCGC 1 cut(s) 470
GluI GCNGC 2 cut(s) 114, 207
GsuI CTGGAG 1 cut(s) 345
HaeIII GGCC 1 cut(s) 196
HapII CCGG 2 cut(s) 210, 605
HgaI GACGC 1 cut(s) 460
HhaI GCGC 1 cut(s) 471
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 64, 170
Hin6I GCGC 1 cut(s) 469
HinP1I GCGC 1 cut(s) 469
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HindIII AAGCTT 1 cut(s) 439
HinfI GANTC 1 cut(s) 281
HpaII CCGG 2 cut(s) 210, 605
Hpy166II GTNNAC 4 cut(s) 12, 302, 316, 600
Hpy188I TCNGA 2 cut(s) 540, 652
Hpy8I GTNNAC 4 cut(s) 12, 302, 316, 600
Hpy99I CGWCG 2 cut(s) 10, 37
HpyAV CCTTC 1 cut(s) 541
HpyCH4III ACNGT 2 cut(s) 28, 544
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 5 cut(s) 64, 87, 166, 182, 620
HpyF10VI GCNNNNNNNGC 3 cut(s) 233, 377, 626
HpySE526I ACGT 1 cut(s) 318
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 64, 170
HspAI GCGC 1 cut(s) 469
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 2 cut(s) 349, 558
LmnI GCTCC 1 cut(s) 385
Lsp1109I GCAGC 1 cut(s) 218
LweI GCATC 1 cut(s) 236
MaeII ACGT 1 cut(s) 318
MaeIII GTNAC 1 cut(s) 472
MalI GATC 2 cut(s) 351, 560
MboI GATC 2 cut(s) 349, 558
MboII GAAGA 2 cut(s) 251, 593
MluCI AATT 5 cut(s) 37, 364, 494, 614, 654
MlyI GAGTC 1 cut(s) 290
MnlI CCTC 2 cut(s) 55, 407
MseI TTAA 1 cut(s) 624
MslI CAYNNNNRTG 2 cut(s) 122, 161
MspI CCGG 2 cut(s) 210, 605
MspR9I CCNGG 3 cut(s) 193, 393, 606
MvaI CCWGG 2 cut(s) 193, 393
MvnI CGCG 1 cut(s) 471
MwoI GCNNNNNNNGC 3 cut(s) 233, 377, 626
NciI CCSGG 1 cut(s) 606
NdeII GATC 2 cut(s) 349, 558
NlaIII CATG 2 cut(s) 64, 170
NlaIV GGNNCC 1 cut(s) 432
NmuCI GTSAC 1 cut(s) 472
OliI CACNNNNGTG 1 cut(s) 161
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PfoI TCCNGGA 1 cut(s) 391
PkrI GCNGC 2 cut(s) 115, 208
PleI GAGTC 1 cut(s) 289
PpsI GAGTC 1 cut(s) 289
Psp6I CCWGG 2 cut(s) 191, 391
PspGI CCWGG 2 cut(s) 191, 391
PspN4I GGNNCC 1 cut(s) 432
RseI CAYNNNNRTG 2 cut(s) 122, 161
SaqAI TTAA 1 cut(s) 624
SatI GCNGC 2 cut(s) 114, 207
Sau3AI GATC 2 cut(s) 349, 558
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 3 cut(s) 193, 393, 606
SetI ASST 9 cut(s) 140, 220, 321, 357, 382, 432, 443, 506, 671
SfaNI GCATC 1 cut(s) 236
SfcI CTRYAG 1 cut(s) 630
SmiMI CAYNNNNRTG 2 cut(s) 122, 161
Sse9I AATT 5 cut(s) 37, 364, 494, 614, 654
SsiI CCGC 2 cut(s) 114, 236
StyD4I CCNGG 3 cut(s) 191, 391, 604
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 2 cut(s) 28, 544
TaiI ACGT 1 cut(s) 321
TaqI TCGA 2 cut(s) 35, 279
TasI AATT 5 cut(s) 37, 364, 494, 614, 654
TauI GCSGC 1 cut(s) 116
Tru1I TTAA 1 cut(s) 624
Tru9I TTAA 1 cut(s) 624
TscAI CASTG 1 cut(s) 89
TseFI GTSAC 1 cut(s) 472
TseI GCWGC 1 cut(s) 206
Tsp45I GTSAC 1 cut(s) 472
TspDTI ATGAA 2 cut(s) 345, 627
TspGWI ACGGA 1 cut(s) 21
TspRI CASTG 1 cut(s) 89
XapI RAATTY 3 cut(s) 37, 364, 614
XmiI GTMKAC 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.