FvH4_4g10850

Domain of unknown function (DUF4504)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012507
Physical Location & Seq
Forward (+)
14543854 .. 14550738
6885 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g10850.t2

Sequence Viewer

Length: 957 bp
ATGAGCAACCCCGGGGCTGGAGTGGTGTATTCGATGGAAATGGAGAAGGAATTCTCAAGTGAAGTCGCAATAAGCGTGTTGGATTCCTCCCTCTCTCGTATCAAATGGCGACTCAAGTCTTCCAGCAGGCGTCGACTAGAGATAGATATAGTGGCCTTGTGTACAGGAATGAGGGCAGTGATAATGGTAGACTACGGTGGCAAGATGCCTGAACTGCAGGACAGGCTCTGCACTCTCCTAAAACTTTGTCGGAAGGGCGCACCATTTTTTGAGCAGCTGAGAGTAATGGTTATAGAAGAAATGATATATTTGGTTCACATTAGTGGACTTGCTAAGTATGTTAGGTCAAGCTTGAACTCCGAAGCACAGCTTCTCTTTGTGGACCTTGAACATGATCCTCCAAAGATGATAAAACAAGCAGAAGAAAACCCACTTGGTGTGCAGCTTATATCAATTCAGAAGTTGTTCTCTTTGGATTTTCCTGTTGGTGGAATGAAAGATGACACATCGTCATCTCATAGTATAGATATGGCTGATTCTGACCCTGTCAGAAATAAACCTATCTCTTCTCACTCCTTTGAGATTATTGATTTCAGTAGCTGCATGCAAGATACTGACCTCACCTTGCCAACATTAAATGGATGGCTTCTTGGTTATCCAGTTGTGTACTTGTTCAGCAAGGAGCATGTAGCTGAAGCTATTTATAATCTTTCTATGAAGTATCTTCATATTTATACAATATCAGTCTGCAGGAATGGAACCCCCAATAAACAATCTGAACTAGAAGAACTCTTGAGTTTCTCAGTGCCTTATGGTTTAAGCATGAGAGGGAGCAAAGAAGAATGGGCAGAGGCATTTTTGGCTCATCTGCAGGAGAAGTGGGAAAGATGCAAGAATGCATGGAAGTCTTTGCGCCTGGAGGTTAGTGAATTAAATCCACAGGCCATTGTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4504

Protein Analysis

319

Amino Acids

36.03

Weight (kDa)

5.6

Isoelectric Point (pI)

50.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4504 PF14953 29 - 264 2.6e-68 Domain of unknown function (DUF4504)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012507)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59490 AT3G59490 AT3G59490 AT3G59490
fragaria_vesca FvH4_4g10850 FvH4_4g10850
malus_domestica MD04G1042500.v1.1
prunus_persica Prupe.1G196900_v2.0.a1
pyrus_communis pycom04g03740
rosa_chinensis RchiOBHm_Chr4g0406631
rosa_multiflora Rmu_sc0002774.1_g000005
rosa_roxburghii Rroxscaffold_5G00351470
rosa_rugosa Rorug04G0072400 Rorug04G0072500
rosa_samantha Rh4AG139700 Rh4BG136700 Rh4CG146900 Rh4DG133900
rosa_wichuraiana Rw4G011380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 705
AccI GTMKAC 2 cut(s) 133, 189
AclWI GGATC 1 cut(s) 389
AcsI RAATTY 1 cut(s) 50
AcuI CTGAAG 1 cut(s) 714
AcyI GRCGYC 1 cut(s) 130
AdeI CACNNNGTG 1 cut(s) 437
AfaI GTAC 2 cut(s) 163, 668
AfiI CCNNNNNNNGG 2 cut(s) 17, 488
AgsI TTSAA 2 cut(s) 355, 389
AhdI GACNNNNNGTC 1 cut(s) 508
AjnI CCWGG 1 cut(s) 915
AjuI GAANNNNNNNTTGG 2 cut(s) 417, 449
AleI CACNNNNGTG 1 cut(s) 321
AluBI AGCT 7 cut(s) 277, 351, 370, 445, 600, 692, 698
AluI AGCT 7 cut(s) 277, 351, 370, 445, 600, 692, 698
AlwI GGATC 1 cut(s) 389
AlwNI CAGNNNCTG 2 cut(s) 228, 600
Ama87I CYCGRG 1 cut(s) 11
AoxI GGCC 2 cut(s) 153, 942
ApeKI GCWGC 3 cut(s) 274, 442, 600
ApoI RAATTY 1 cut(s) 50
Asp700I GAANNNNTTC 2 cut(s) 50, 464
AspLEI GCGC 2 cut(s) 260, 915
AspS9I GGNCC 1 cut(s) 382
AsuC2I CCSGG 2 cut(s) 12, 13
AsuHPI GGTGA 1 cut(s) 613
AvaI CYCGRG 1 cut(s) 11
AvaII GGWCC 1 cut(s) 382
BbsI GAAGAC 1 cut(s) 111
BbvI GCAGC 3 cut(s) 286, 454, 587
BccI CCATC 2 cut(s) 28, 636
BciT130I CCWGG 1 cut(s) 917
BcnI CCSGG 2 cut(s) 12, 13
BfaI CTAG 2 cut(s) 137, 782
BfmI CTRYAG 3 cut(s) 215, 748, 869
BisI GCNGC 3 cut(s) 275, 443, 601
BlsI GCNGC 3 cut(s) 276, 444, 602
Bme1390I CCNGG 3 cut(s) 12, 13, 917
Bme18I GGWCC 1 cut(s) 382
BmeRI GACNNNNNGTC 1 cut(s) 508
BmeT110I CYCGRG 1 cut(s) 11
BmgT120I GGNCC 1 cut(s) 382
BmiI GGNNCC 1 cut(s) 760
BmrFI CCNGG 3 cut(s) 12, 13, 917
BmsI GCATC 2 cut(s) 195, 878
BoxI GACNNNNGTC 1 cut(s) 115
BpiI GAAGAC 1 cut(s) 111
BpmI CTGGAG 2 cut(s) 39, 938
BpuEI CTTGAG 3 cut(s) 40, 98, 814
BpuMI CCSGG 2 cut(s) 12, 13
BsaHI GRCGYC 1 cut(s) 130
BsaJI CCNNGG 3 cut(s) 10, 11, 12
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 488
Bse1I ACTGG 1 cut(s) 659
BseBI CCWGG 1 cut(s) 917
BseDI CCNNGG 3 cut(s) 10, 11, 12
BseGI GGATG 1 cut(s) 647
BseLI CCNNNNNNNGG 2 cut(s) 17, 488
BseMII CTCAG 2 cut(s) 269, 816
BseNI ACTGG 1 cut(s) 659
BseXI GCAGC 3 cut(s) 286, 454, 587
BsgI GTGCAG 2 cut(s) 214, 461
BshFI GGCC 2 cut(s) 155, 944
BsiHKCI CYCGRG 1 cut(s) 11
BsiSI CCGG 1 cut(s) 12
BslI CCNNNNNNNGG 2 cut(s) 17, 488
BsmI GAATGC 1 cut(s) 901
BsnI GGCC 2 cut(s) 155, 944
BsoBI CYCGRG 1 cut(s) 11
Bsp1407I TGTACA 1 cut(s) 161
Bsp143I GATC 1 cut(s) 394
BspANI GGCC 2 cut(s) 155, 944
BspCNI CTCAG 2 cut(s) 270, 815
BspLI GGNNCC 1 cut(s) 760
BspMAI CTGCAG 3 cut(s) 219, 752, 873
BspPI GGATC 1 cut(s) 389
BsrGI TGTACA 1 cut(s) 161
BsrI ACTGG 1 cut(s) 659
BssECI CCNNGG 3 cut(s) 10, 11, 12
BssMI GATC 1 cut(s) 394
BssNI GRCGYC 1 cut(s) 130
Bst2UI CCWGG 1 cut(s) 917
Bst4CI ACNGT 1 cut(s) 197
Bst6I CTCTTC 1 cut(s) 571
BstACI GRCGYC 1 cut(s) 130
BstAUI TGTACA 1 cut(s) 161
BstC8I GCNNGC 2 cut(s) 128, 605
BstDEI CTNAG 3 cut(s) 278, 333, 802
BstF5I GGATG 1 cut(s) 647
BstHHI GCGC 2 cut(s) 260, 915
BstKTI GATC 1 cut(s) 397
BstMBI GATC 1 cut(s) 394
BstMWI GCNNNNNNNGC 3 cut(s) 214, 223, 860
BstNI CCWGG 1 cut(s) 917
BstNSI RCATGY 2 cut(s) 607, 689
BstPAI GACNNNNGTC 1 cut(s) 115
BstSCI CCNGG 3 cut(s) 10, 11, 915
BstSFI CTRYAG 3 cut(s) 215, 748, 869
BstV1I GCAGC 3 cut(s) 286, 454, 587
BstV2I GAAGAC 1 cut(s) 111
BsuRI GGCC 2 cut(s) 155, 944
BtsCI GGATG 1 cut(s) 647
BtsI GCAGTG 1 cut(s) 183
BtsIMutI CAGTG 2 cut(s) 183, 810
Cac8I GCNNGC 2 cut(s) 128, 605
CaiI CAGNNNCTG 2 cut(s) 228, 600
CfoI GCGC 2 cut(s) 260, 915
Cfr13I GGNCC 1 cut(s) 382
Cfr9I CCCGGG 1 cut(s) 11
CseI GACGC 1 cut(s) 119
Csp6I GTAC 2 cut(s) 162, 667
CviAII CATG 5 cut(s) 392, 604, 686, 823, 900
CviQI GTAC 2 cut(s) 162, 667
DdeI CTNAG 3 cut(s) 278, 333, 802
DpnI GATC 1 cut(s) 396
DpnII GATC 1 cut(s) 394
DraIII CACNNNGTG 1 cut(s) 437
DriI GACNNNNNGTC 1 cut(s) 508
Eam1104I CTCTTC 1 cut(s) 571
Eam1105I GACNNNNNGTC 1 cut(s) 508
EarI CTCTTC 1 cut(s) 571
Eco47I GGWCC 1 cut(s) 382
Eco57I CTGAAG 1 cut(s) 714
Eco88I CYCGRG 1 cut(s) 11
EcoRI GAATTC 1 cut(s) 50
EcoRII CCWGG 1 cut(s) 915
EcoT22I ATGCAT 1 cut(s) 901
FaeI CATG 5 cut(s) 395, 607, 689, 826, 903
FalI AAGNNNNNCTT 2 cut(s) 354, 386
FatI CATG 5 cut(s) 391, 603, 685, 822, 899
FblI GTMKAC 2 cut(s) 133, 189
Fnu4HI GCNGC 3 cut(s) 275, 443, 601
FokI GGATG 1 cut(s) 654
Fsp4HI GCNGC 3 cut(s) 275, 443, 601
FspBI CTAG 2 cut(s) 137, 782
GlaI GCGC 2 cut(s) 259, 914
GluI GCNGC 3 cut(s) 275, 443, 601
GsuI CTGGAG 2 cut(s) 39, 938
HaeIII GGCC 2 cut(s) 155, 944
HapII CCGG 1 cut(s) 12
HgaI GACGC 1 cut(s) 119
HhaI GCGC 2 cut(s) 260, 915
Hin1I GRCGYC 1 cut(s) 130
Hin1II CATG 5 cut(s) 395, 607, 689, 826, 903
Hin6I GCGC 2 cut(s) 258, 913
HinP1I GCGC 2 cut(s) 258, 913
HincII GTYRAC 1 cut(s) 134
HindII GTYRAC 1 cut(s) 134
HindIII AAGCTT 1 cut(s) 349
HinfI GANTC 3 cut(s) 83, 111, 536
HpaII CCGG 1 cut(s) 12
HphI GGTGA 1 cut(s) 613
Hpy166II GTNNAC 7 cut(s) 134, 162, 190, 316, 326, 382, 667
Hpy188I TCNGA 6 cut(s) 252, 361, 459, 541, 551, 778
Hpy188III TCNNGA 1 cut(s) 793
Hpy8I GTNNAC 7 cut(s) 134, 162, 190, 316, 326, 382, 667
Hpy99I CGWCG 1 cut(s) 135
HpyAV CCTTC 2 cut(s) 40, 247
HpyCH4III ACNGT 1 cut(s) 197
HpyCH4V TGCA 9 cut(s) 217, 231, 442, 603, 607, 750, 871, 891, 899
HpyF10VI GCNNNNNNNGC 3 cut(s) 214, 223, 860
HpyF3I CTNAG 3 cut(s) 278, 333, 802
Hsp92I GRCGYC 1 cut(s) 130
Hsp92II CATG 5 cut(s) 395, 607, 689, 826, 903
HspAI GCGC 2 cut(s) 258, 913
Kzo9I GATC 1 cut(s) 394
LmnI GCTCC 2 cut(s) 682, 831
Lsp1109I GCAGC 3 cut(s) 286, 454, 587
LweI GCATC 2 cut(s) 195, 878
MaeI CTAG 2 cut(s) 137, 782
MalI GATC 1 cut(s) 396
MboI GATC 1 cut(s) 394
MboII GAAGA 7 cut(s) 111, 308, 434, 558, 716, 797, 851
MluCI AATT 3 cut(s) 50, 453, 929
MlyI GAGTC 1 cut(s) 105
MmeI TCCRAC 2 cut(s) 60, 230
MnlI CCTC 8 cut(s) 97, 101, 165, 408, 629, 821, 844, 913
Mph1103I ATGCAT 1 cut(s) 901
MroXI GAANNNNTTC 2 cut(s) 50, 464
MseI TTAA 3 cut(s) 635, 818, 932
MslI CAYNNNNRTG 1 cut(s) 321
MspA1I CMGCKG 1 cut(s) 277
MspI CCGG 1 cut(s) 12
MspR9I CCNGG 3 cut(s) 12, 13, 917
Mva1269I GAATGC 1 cut(s) 901
MvaI CCWGG 1 cut(s) 917
MwoI GCNNNNNNNGC 3 cut(s) 214, 223, 860
NciI CCSGG 2 cut(s) 12, 13
NdeII GATC 1 cut(s) 394
NlaIII CATG 5 cut(s) 395, 607, 689, 826, 903
NlaIV GGNNCC 1 cut(s) 760
NsiI ATGCAT 1 cut(s) 901
NspI RCATGY 2 cut(s) 607, 689
OliI CACNNNNGTG 1 cut(s) 321
PaeI GCATGC 1 cut(s) 607
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PctI GAATGC 1 cut(s) 901
PdmI GAANNNNTTC 2 cut(s) 50, 464
PfeI GAWTC 2 cut(s) 83, 536
PflFI GACNNNGTC 1 cut(s) 545
PkrI GCNGC 3 cut(s) 276, 444, 602
PleI GAGTC 1 cut(s) 105
PpsI GAGTC 1 cut(s) 105
PshAI GACNNNNGTC 1 cut(s) 115
PsiI TTATAA 1 cut(s) 705
Psp6I CCWGG 1 cut(s) 915
PspGI CCWGG 1 cut(s) 915
PspN4I GGNNCC 1 cut(s) 760
PspPI GGNCC 1 cut(s) 382
PstI CTGCAG 3 cut(s) 219, 752, 873
PstNI CAGNNNCTG 2 cut(s) 228, 600
PsyI GACNNNGTC 1 cut(s) 545
PvuII CAGCTG 1 cut(s) 277
RsaI GTAC 2 cut(s) 163, 668
RsaNI GTAC 2 cut(s) 162, 667
RseI CAYNNNNRTG 1 cut(s) 321
SalI GTCGAC 1 cut(s) 132
SaqAI TTAA 3 cut(s) 635, 818, 932
SatI GCNGC 3 cut(s) 275, 443, 601
Sau3AI GATC 1 cut(s) 394
Sau96I GGNCC 1 cut(s) 382
SchI GAGTC 1 cut(s) 105
ScrFI CCNGG 3 cut(s) 12, 13, 917
SfaNI GCATC 2 cut(s) 195, 878
SfcI CTRYAG 3 cut(s) 215, 748, 869
SinI GGWCC 1 cut(s) 382
SmaI CCCGGG 1 cut(s) 13
SmiMI CAYNNNNRTG 1 cut(s) 321
SmlI CTYRAG 3 cut(s) 55, 113, 793
SmoI CTYRAG 3 cut(s) 55, 113, 793
SphI GCATGC 1 cut(s) 607
Sse9I AATT 3 cut(s) 50, 453, 929
SspMI CTAG 2 cut(s) 137, 782
StyD4I CCNGG 3 cut(s) 10, 11, 915
TaaI ACNGT 1 cut(s) 197
TaqI TCGA 2 cut(s) 32, 133
TasI AATT 3 cut(s) 50, 453, 929
TatI WGTACW 2 cut(s) 161, 666
TfiI GAWTC 2 cut(s) 83, 536
Tru1I TTAA 3 cut(s) 635, 818, 932
Tru9I TTAA 3 cut(s) 635, 818, 932
TscAI CASTG 2 cut(s) 183, 810
TseI GCWGC 3 cut(s) 274, 442, 600
TspDTI ATGAA 3 cut(s) 509, 716, 731
TspMI CCCGGG 1 cut(s) 11
TspRI CASTG 2 cut(s) 183, 810
Tth111I GACNNNGTC 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 382
XapI RAATTY 1 cut(s) 50
XceI RCATGY 2 cut(s) 607, 689
XmaI CCCGGG 1 cut(s) 11
XmiI GTMKAC 2 cut(s) 133, 189
XmnI GAANNNNTTC 2 cut(s) 50, 464
XspI CTAG 2 cut(s) 137, 782
Zsp2I ATGCAT 1 cut(s) 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.