Rroxscaffold_5G00351470

Domain of unknown function (DUF4504)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0012507
Physical Location & Seq
Reverse (-)
27675316 .. 27682855
7540 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00351470.1

Sequence Viewer

Length: 1023 bp
ATGGAAAACGAGAAGGAAGTCTCAAGTGAAGTTGCAGAGTTGGAGGAAGCAATAAACGTGTTGGATTCATCCCTCTCTCTTATCAAATGGCGACTCAAGTATTCAAGCAGACGTCGACTTGAGATAGATATTCTGGCCTTGTGTACTGGAATGAGGCCAGTGATAATGGTAGACTATGGTGGAAAGATGCCTGAACTGCAGGAGAGGCTCTGCAATCTTTTAAAACTTTGTCGAAAGAGCTCAACCATTTTTGAGCAGCTGAGAGTAATGGTTATAGAAGAAATGATATATTTGGTGCACATTAGTGAACTTGCTGATTATGTTAGGTCAAGCTTGAACTCTGAAGCAAAGCTTCTCTTTGTGGACCTTGAACATGATCCTCCAAAGATGATAAAACAAGCGGAGAAACACCAACTAGGTGTGCAGCTTTTATCAATTCAGAAGTTGTTCTCTTTAAATTTTCCTGCTGGTGGAATGAAAGATGACACATCGTCATCTCATAGTATAGATATGGTTGATTCTAAACCTGCCAGAGATGAACTGATCTCTTCTCACTCCTCCGAGATTATTGATTTCAGTACCTGCATGCAAGATACTCAGGTCACCTTGCCAACATTAAATGGATGGCTTCTTGGTTATCCAGTGGTGTACTTGTTCAGCAAGGAGCATGTAGCTGATGCTGTTTATAATCTTTCTATGAAGTATCTTCATATTTATACAGTATCAGTCTGCAGGAATGGGACCCCCAATAAACAATCTGAGCTAGAAGAACTCTTGAGTTTCTCGGTGCCTTACGGTTTAAGCTTAAGAGGGAGCAAAGAAGAATGGGCCGAGGCATTGTTGGCTCATCTGCAGGAGAAGTGGGAAAGATGCAAGAATGCTTGGAGGTCTTTGCAGTTGGAGATTAAAGTTGGTAGAGGAAAATTGACTGACAGGAATGAGACTGGACTAACATGTGGAATGGAAACTAGCAAATCCTTTTCGGACTATGCCTTCGGGTTTCTTATGGTGGTTGTGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4504

Protein Analysis

340

Amino Acids

38.75

Weight (kDa)

5.51

Isoelectric Point (pI)

45.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4504 PF14953 24 - 303 1.1e-84 Domain of unknown function (DUF4504)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012507)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59490 AT3G59490 AT3G59490 AT3G59490
fragaria_vesca FvH4_4g10850 FvH4_4g10850
malus_domestica MD04G1042500.v1.1
prunus_persica Prupe.1G196900_v2.0.a1
pyrus_communis pycom04g03740
rosa_chinensis RchiOBHm_Chr4g0406631
rosa_multiflora Rmu_sc0002774.1_g000005
rosa_roxburghii Rroxscaffold_5G00351470
rosa_rugosa Rorug04G0072400 Rorug04G0072500
rosa_samantha Rh4AG139700 Rh4BG136700 Rh4CG146900 Rh4DG133900
rosa_wichuraiana Rw4G011380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 687
AatII GACGTC 1 cut(s) 115
Acc36I ACCTGC 2 cut(s) 535, 590
AccB1I GGYRCC 1 cut(s) 787
AccI GTMKAC 2 cut(s) 115, 171
AciI CCGC 1 cut(s) 401
AclWI GGATC 1 cut(s) 371
AcsI RAATTY 1 cut(s) 457
AcuI CTGAAG 1 cut(s) 363
AcyI GRCGYC 1 cut(s) 112
AfaI GTAC 3 cut(s) 145, 580, 650
AfiI CCNNNNNNNGG 1 cut(s) 470
AflII CTTAAG 1 cut(s) 805
AflIII ACRYGT 2 cut(s) 57, 953
AgsI TTSAA 3 cut(s) 105, 337, 371
AhdI GACNNNNNGTC 1 cut(s) 490
AleI CACNNNNGTG 1 cut(s) 303
AluBI AGCT 8 cut(s) 240, 259, 333, 352, 427, 674, 763, 804
AluI AGCT 8 cut(s) 240, 259, 333, 352, 427, 674, 763, 804
Alw21I GWGCWC 2 cut(s) 242, 300
Alw26I GTCTC 2 cut(s) 25, 935
Alw44I GTGCAC 1 cut(s) 296
AlwI GGATC 1 cut(s) 371
AlwNI CAGNNNCTG 1 cut(s) 582
AoxI GGCC 3 cut(s) 135, 155, 828
ApaLI GTGCAC 1 cut(s) 296
ApeKI GCWGC 2 cut(s) 256, 424
ApoI RAATTY 1 cut(s) 457
ArsI GACNNNNNNTTYG 2 cut(s) 977, 1009
Asp700I GAANNNNTTC 1 cut(s) 446
AspS9I GGNCC 3 cut(s) 364, 741, 828
AsuHPI GGTGA 1 cut(s) 595
AvaII GGWCC 2 cut(s) 364, 741
BaeGI GKGCMC 1 cut(s) 300
BaeI ACNNNNGTAYC 2 cut(s) 585, 618
BanI GGYRCC 1 cut(s) 787
BanII GRGCYC 1 cut(s) 242
Bbv12I GWGCWC 2 cut(s) 242, 300
BbvI GCAGC 2 cut(s) 268, 436
BccI CCATC 1 cut(s) 618
BcoDI GTCTC 2 cut(s) 25, 935
BfaI CTAG 3 cut(s) 416, 764, 969
BfmI CTRYAG 3 cut(s) 197, 730, 851
BfrI CTTAAG 1 cut(s) 805
BfuAI ACCTGC 2 cut(s) 535, 590
BisI GCNGC 2 cut(s) 257, 425
BlsI GCNGC 2 cut(s) 258, 426
Bme18I GGWCC 2 cut(s) 364, 741
BmeRI GACNNNNNGTC 1 cut(s) 490
BmgT120I GGNCC 3 cut(s) 364, 741, 828
BmiI GGNNCC 3 cut(s) 742, 743, 789
BmsI GCATC 3 cut(s) 177, 667, 860
BpuEI CTTGAG 4 cut(s) 7, 80, 140, 796
BsaHI GRCGYC 1 cut(s) 112
BsaJI CCNNGG 1 cut(s) 831
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse1I ACTGG 4 cut(s) 151, 158, 641, 949
BseDI CCNNGG 1 cut(s) 831
BseGI GGATG 2 cut(s) 68, 629
BseLI CCNNNNNNNGG 1 cut(s) 470
BseMII CTCAG 3 cut(s) 251, 611, 750
BseNI ACTGG 4 cut(s) 151, 158, 641, 949
BseRI GAGGAG 1 cut(s) 547
BseSI GKGCMC 1 cut(s) 300
BseXI GCAGC 2 cut(s) 268, 436
BsgI GTGCAG 1 cut(s) 443
BshFI GGCC 3 cut(s) 137, 157, 830
BshNI GGYRCC 1 cut(s) 787
BsiHKAI GWGCWC 2 cut(s) 242, 300
BslFI GGGAC 1 cut(s) 754
BslI CCNNNNNNNGG 1 cut(s) 470
BsmAI GTCTC 2 cut(s) 25, 935
BsmFI GGGAC 1 cut(s) 754
BsmI GAATGC 1 cut(s) 883
BsnI GGCC 3 cut(s) 137, 157, 830
Bsp1286I GDGCHC 2 cut(s) 242, 300
Bsp143I GATC 2 cut(s) 376, 543
BspACI CCGC 1 cut(s) 401
BspANI GGCC 3 cut(s) 137, 157, 830
BspCNI CTCAG 3 cut(s) 252, 610, 751
BspLI GGNNCC 3 cut(s) 742, 743, 789
BspMAI CTGCAG 3 cut(s) 201, 734, 855
BspMI ACCTGC 2 cut(s) 535, 590
BspPI GGATC 1 cut(s) 371
BspT107I GGYRCC 1 cut(s) 787
BspTI CTTAAG 1 cut(s) 805
BsrI ACTGG 4 cut(s) 151, 158, 641, 949
BssECI CCNNGG 1 cut(s) 831
BssMI GATC 2 cut(s) 376, 543
BssNI GRCGYC 1 cut(s) 112
Bst4CI ACNGT 2 cut(s) 721, 797
Bst6I CTCTTC 1 cut(s) 553
BstACI GRCGYC 1 cut(s) 112
BstAFI CTTAAG 1 cut(s) 805
BstC8I GCNNGC 1 cut(s) 587
BstDEI CTNAG 3 cut(s) 260, 597, 759
BstEII GGTNACC 1 cut(s) 601
BstF5I GGATG 2 cut(s) 68, 629
BstKTI GATC 2 cut(s) 379, 546
BstMAI GTCTC 2 cut(s) 25, 935
BstMBI GATC 2 cut(s) 376, 543
BstMWI GCNNNNNNNGC 3 cut(s) 196, 205, 842
BstNSI RCATGY 3 cut(s) 589, 671, 957
BstPI GGTNACC 1 cut(s) 601
BstSFI CTRYAG 3 cut(s) 197, 730, 851
BstSLI GKGCMC 1 cut(s) 300
BstV1I GCAGC 2 cut(s) 268, 436
BsuRI GGCC 3 cut(s) 137, 157, 830
BtsCI GGATG 2 cut(s) 68, 629
BtsIMutI CAGTG 2 cut(s) 165, 648
BveI ACCTGC 2 cut(s) 535, 590
Cac8I GCNNGC 1 cut(s) 587
CaiI CAGNNNCTG 1 cut(s) 582
Cfr13I GGNCC 3 cut(s) 364, 741, 828
Csp6I GTAC 3 cut(s) 144, 579, 649
CviAII CATG 4 cut(s) 374, 586, 668, 954
CviQI GTAC 3 cut(s) 144, 579, 649
DdeI CTNAG 3 cut(s) 260, 597, 759
DpnI GATC 2 cut(s) 378, 545
DpnII GATC 2 cut(s) 376, 543
DraI TTTAAA 2 cut(s) 222, 456
DriI GACNNNNNGTC 1 cut(s) 490
Eam1104I CTCTTC 1 cut(s) 553
Eam1105I GACNNNNNGTC 1 cut(s) 490
EarI CTCTTC 1 cut(s) 553
Ecl136II GAGCTC 1 cut(s) 240
Eco24I GRGCYC 1 cut(s) 242
Eco47I GGWCC 2 cut(s) 364, 741
Eco53kI GAGCTC 1 cut(s) 240
Eco57I CTGAAG 1 cut(s) 363
Eco91I GGTNACC 1 cut(s) 601
EcoICRI GAGCTC 1 cut(s) 240
EcoO109I RGGNCCY 1 cut(s) 741
EcoO65I GGTNACC 1 cut(s) 601
EcoT38I GRGCYC 1 cut(s) 242
FaeI CATG 4 cut(s) 377, 589, 671, 957
FalI AAGNNNNNCTT 4 cut(s) 336, 368, 341, 373
FaqI GGGAC 1 cut(s) 754
FatI CATG 4 cut(s) 373, 585, 667, 953
FblI GTMKAC 2 cut(s) 115, 171
Fnu4HI GCNGC 2 cut(s) 257, 425
FokI GGATG 2 cut(s) 55, 636
FriOI GRGCYC 1 cut(s) 242
Fsp4HI GCNGC 2 cut(s) 257, 425
FspBI CTAG 3 cut(s) 416, 764, 969
GluI GCNGC 2 cut(s) 257, 425
HaeIII GGCC 3 cut(s) 137, 157, 830
Hin1I GRCGYC 1 cut(s) 112
Hin1II CATG 4 cut(s) 377, 589, 671, 957
HincII GTYRAC 1 cut(s) 116
HindII GTYRAC 1 cut(s) 116
HindIII AAGCTT 3 cut(s) 331, 350, 802
HinfI GANTC 3 cut(s) 65, 93, 518
HphI GGTGA 1 cut(s) 595
Hpy166II GTNNAC 7 cut(s) 116, 144, 172, 298, 308, 364, 649
Hpy188I TCNGA 5 cut(s) 343, 441, 562, 760, 985
Hpy188III TCNNGA 1 cut(s) 775
Hpy8I GTNNAC 7 cut(s) 116, 144, 172, 298, 308, 364, 649
Hpy99I CGWCG 1 cut(s) 117
HpyAV CCTTC 2 cut(s) 7, 1003
HpyCH4III ACNGT 2 cut(s) 721, 797
HpyCH4IV ACGT 2 cut(s) 57, 112
HpyF10VI GCNNNNNNNGC 3 cut(s) 196, 205, 842
HpyF3I CTNAG 3 cut(s) 260, 597, 759
HpySE526I ACGT 2 cut(s) 57, 112
Hsp92I GRCGYC 1 cut(s) 112
Hsp92II CATG 4 cut(s) 377, 589, 671, 957
KflI GGGWCCC 1 cut(s) 741
Kzo9I GATC 2 cut(s) 376, 543
LmnI GCTCC 2 cut(s) 664, 813
Lsp1109I GCAGC 2 cut(s) 268, 436
LweI GCATC 3 cut(s) 177, 667, 860
MaeI CTAG 3 cut(s) 416, 764, 969
MaeII ACGT 2 cut(s) 57, 112
MaeIII GTNAC 1 cut(s) 601
MalI GATC 2 cut(s) 378, 545
MboI GATC 2 cut(s) 376, 543
MboII GAAGA 5 cut(s) 290, 540, 698, 779, 833
MhlI GDGCHC 2 cut(s) 242, 300
MluCI AATT 3 cut(s) 435, 457, 923
MlyI GAGTC 1 cut(s) 87
MmeI TCCRAC 3 cut(s) 21, 42, 879
MroXI GAANNNNTTC 1 cut(s) 446
MseI TTAA 6 cut(s) 221, 455, 617, 800, 806, 906
MslI CAYNNNNRTG 1 cut(s) 303
MspA1I CMGCKG 1 cut(s) 259
MspCI CTTAAG 1 cut(s) 805
Mva1269I GAATGC 1 cut(s) 883
MwoI GCNNNNNNNGC 3 cut(s) 196, 205, 842
NdeII GATC 2 cut(s) 376, 543
NlaIII CATG 4 cut(s) 377, 589, 671, 957
NlaIV GGNNCC 3 cut(s) 742, 743, 789
NmeAIII GCCGAG 1 cut(s) 856
NmuCI GTSAC 1 cut(s) 601
NspI RCATGY 3 cut(s) 589, 671, 957
OliI CACNNNNGTG 1 cut(s) 303
PaeI GCATGC 1 cut(s) 589
PciI ACATGT 1 cut(s) 953
PctI GAATGC 1 cut(s) 883
PdmI GAANNNNTTC 1 cut(s) 446
PfeI GAWTC 2 cut(s) 65, 518
PkrI GCNGC 2 cut(s) 258, 426
PleI GAGTC 1 cut(s) 87
PpsI GAGTC 1 cut(s) 87
PpuMI RGGWCCY 1 cut(s) 741
PscI ACATGT 1 cut(s) 953
PsiI TTATAA 1 cut(s) 687
Psp124BI GAGCTC 1 cut(s) 242
Psp5II RGGWCCY 1 cut(s) 741
PspEI GGTNACC 1 cut(s) 601
PspN4I GGNNCC 3 cut(s) 742, 743, 789
PspPI GGNCC 3 cut(s) 364, 741, 828
PspPPI RGGWCCY 1 cut(s) 741
PstI CTGCAG 3 cut(s) 201, 734, 855
PstNI CAGNNNCTG 1 cut(s) 582
PvuII CAGCTG 1 cut(s) 259
RsaI GTAC 3 cut(s) 145, 580, 650
RsaNI GTAC 3 cut(s) 144, 579, 649
RseI CAYNNNNRTG 1 cut(s) 303
SacI GAGCTC 1 cut(s) 242
SalI GTCGAC 1 cut(s) 114
SaqAI TTAA 6 cut(s) 221, 455, 617, 800, 806, 906
SatI GCNGC 2 cut(s) 257, 425
Sau3AI GATC 2 cut(s) 376, 543
Sau96I GGNCC 3 cut(s) 364, 741, 828
SchI GAGTC 1 cut(s) 87
SduI GDGCHC 2 cut(s) 242, 300
SfaNI GCATC 3 cut(s) 177, 667, 860
SfcI CTRYAG 3 cut(s) 197, 730, 851
SinI GGWCC 2 cut(s) 364, 741
SmiMI CAYNNNNRTG 1 cut(s) 303
SmlI CTYRAG 5 cut(s) 22, 95, 119, 775, 805
SmoI CTYRAG 5 cut(s) 22, 95, 119, 775, 805
SphI GCATGC 1 cut(s) 589
Sse9I AATT 3 cut(s) 435, 457, 923
SsiI CCGC 1 cut(s) 401
SspMI CTAG 3 cut(s) 416, 764, 969
SstI GAGCTC 1 cut(s) 242
TaaI ACNGT 2 cut(s) 721, 797
TaiI ACGT 2 cut(s) 60, 115
TaqI TCGA 2 cut(s) 115, 232
TasI AATT 3 cut(s) 435, 457, 923
TatI WGTACW 2 cut(s) 143, 648
TfiI GAWTC 2 cut(s) 65, 518
Tru1I TTAA 6 cut(s) 221, 455, 617, 800, 806, 906
Tru9I TTAA 6 cut(s) 221, 455, 617, 800, 806, 906
TscAI CASTG 2 cut(s) 165, 648
TseFI GTSAC 1 cut(s) 601
TseI GCWGC 2 cut(s) 256, 424
Tsp45I GTSAC 1 cut(s) 601
TspDTI ATGAA 5 cut(s) 57, 491, 552, 698, 713
TspRI CASTG 2 cut(s) 165, 648
Vha464I CTTAAG 1 cut(s) 805
VneI GTGCAC 1 cut(s) 296
VpaK11BI GGWCC 2 cut(s) 364, 741
XapI RAATTY 1 cut(s) 457
XceI RCATGY 3 cut(s) 589, 671, 957
XmiI GTMKAC 2 cut(s) 115, 171
XmnI GAANNNNTTC 1 cut(s) 446
XspI CTAG 3 cut(s) 416, 764, 969
ZraI GACGTC 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.