Rh4DG133900

Domain of unknown function (DUF4504)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0012507
Physical Location & Seq
Reverse (-)
26662994 .. 26669993
7000 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG133900.1

Sequence Viewer

Length: 774 bp
ATGGAAAACGAGAAGGAATTCTCAAGTGAAGTTGCGGAGTTGGAGGAAGCAATAAACGTGTTGGATTCATCCCTCTCTCTTAGCAAATGGCGACTCAAGTATTCAAGCAGACGTCGACTTGAGATAGATATTCTGGCCTTGTGTACTGGAATGAGGCCAGTGATAATGGTAGACTATGGTGGAAAGATGCCTGAACTGCAGGAGAGGCTCTGCAATCTTTTAAAACTTTGTCGAAAGAGCTCAACCATTTTTGAGCAGCTGAGAGTAATGGTTATAGAAGAAATGATATATTTGGTGCACATTAGTGAACTTGCTGATTATGTTAGGTCAAGCTTGAACTCTGAAGCAAAGCTTCTCTTTGTGGACCTTGAACATGATCCTCCAAAGATGATAAAACAAGCGGAGAAACACCAACTAGGTGTGCAGCTTATATCAATTCAGAAGTTGTTCTCTTTAAATTTTCCTTCTGGTGGAATGAAAGATGACACATCGTCATCGTCATCTCATAGTATAGATATGGTTGATTCTAAACCTGCCAGAAATGAACTGATCTCTTCTCACTCCTCCGAGATTATTGATTTCAGTACGTGCATGCAAGATACTCAGGTTACCTTGCCAACATTAAATGGATGGCTTCTTGGTTATCCAGTGGTGTACTTGTTCAGCAAGGAGCATGTAGCTGATGCTGTTTATAATCTTTCTATGAAGTATCTTCATATTTATACAATATCAGTCTGCAGGTTGGTTGTTCTCGTCTTTGTTTCATTCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4504

Protein Analysis

257

Amino Acids

29.25

Weight (kDa)

5.54

Isoelectric Point (pI)

49.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4504 PF14953 24 - 247 4.1e-68 Domain of unknown function (DUF4504)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012507)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59490 AT3G59490 AT3G59490 AT3G59490
fragaria_vesca FvH4_4g10850 FvH4_4g10850
malus_domestica MD04G1042500.v1.1
prunus_persica Prupe.1G196900_v2.0.a1
pyrus_communis pycom04g03740
rosa_chinensis RchiOBHm_Chr4g0406631
rosa_multiflora Rmu_sc0002774.1_g000005
rosa_roxburghii Rroxscaffold_5G00351470
rosa_rugosa Rorug04G0072400 Rorug04G0072500
rosa_samantha Rh4AG139700 Rh4BG136700 Rh4CG146900 Rh4DG133900
rosa_wichuraiana Rw4G011380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 693
AatII GACGTC 1 cut(s) 115
Acc36I ACCTGC 2 cut(s) 541, 729
AccI GTMKAC 2 cut(s) 115, 171
AciI CCGC 2 cut(s) 35, 401
AclWI GGATC 1 cut(s) 371
AcsI RAATTY 2 cut(s) 17, 457
AcuI CTGAAG 1 cut(s) 363
AcyI GRCGYC 1 cut(s) 112
AfaI GTAC 3 cut(s) 145, 586, 656
AfiI CCNNNNNNNGG 1 cut(s) 470
AflIII ACRYGT 1 cut(s) 57
AgsI TTSAA 3 cut(s) 105, 337, 371
AhdI GACNNNNNGTC 1 cut(s) 490
AleI CACNNNNGTG 1 cut(s) 303
AluBI AGCT 6 cut(s) 240, 259, 333, 352, 427, 680
AluI AGCT 6 cut(s) 240, 259, 333, 352, 427, 680
Alw21I GWGCWC 2 cut(s) 242, 300
Alw44I GTGCAC 1 cut(s) 296
AlwI GGATC 1 cut(s) 371
AoxI GGCC 2 cut(s) 135, 155
ApaLI GTGCAC 1 cut(s) 296
ApeKI GCWGC 2 cut(s) 256, 424
ApoI RAATTY 2 cut(s) 17, 457
Asp700I GAANNNNTTC 2 cut(s) 17, 446
AspS9I GGNCC 1 cut(s) 364
AvaII GGWCC 1 cut(s) 364
BaeGI GKGCMC 1 cut(s) 300
BaeI ACNNNNGTAYC 2 cut(s) 591, 624
BanII GRGCYC 1 cut(s) 242
Bbv12I GWGCWC 2 cut(s) 242, 300
BbvI GCAGC 2 cut(s) 268, 436
BccI CCATC 1 cut(s) 624
BfaI CTAG 1 cut(s) 416
BfmI CTRYAG 2 cut(s) 197, 736
BfuAI ACCTGC 2 cut(s) 541, 729
BisI GCNGC 2 cut(s) 257, 425
BlsI GCNGC 2 cut(s) 258, 426
Bme18I GGWCC 1 cut(s) 364
BmeRI GACNNNNNGTC 1 cut(s) 490
BmgT120I GGNCC 1 cut(s) 364
BmsI GCATC 2 cut(s) 177, 673
BpuEI CTTGAG 3 cut(s) 7, 80, 140
BsaAI YACGTR 1 cut(s) 588
BsaHI GRCGYC 1 cut(s) 112
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse1I ACTGG 3 cut(s) 151, 158, 647
BseGI GGATG 2 cut(s) 68, 635
BseLI CCNNNNNNNGG 1 cut(s) 470
BseMII CTCAG 2 cut(s) 251, 617
BseNI ACTGG 3 cut(s) 151, 158, 647
BseRI GAGGAG 1 cut(s) 553
BseSI GKGCMC 1 cut(s) 300
BseXI GCAGC 2 cut(s) 268, 436
BsgI GTGCAG 1 cut(s) 443
BshFI GGCC 2 cut(s) 137, 157
BsiHKAI GWGCWC 2 cut(s) 242, 300
BslI CCNNNNNNNGG 1 cut(s) 470
BsnI GGCC 2 cut(s) 137, 157
Bsp1286I GDGCHC 2 cut(s) 242, 300
Bsp143I GATC 2 cut(s) 376, 549
BspACI CCGC 2 cut(s) 35, 401
BspANI GGCC 2 cut(s) 137, 157
BspCNI CTCAG 2 cut(s) 252, 616
BspMAI CTGCAG 2 cut(s) 201, 740
BspMI ACCTGC 2 cut(s) 541, 729
BspPI GGATC 1 cut(s) 371
BsrI ACTGG 3 cut(s) 151, 158, 647
BssMI GATC 2 cut(s) 376, 549
BssNI GRCGYC 1 cut(s) 112
Bst6I CTCTTC 1 cut(s) 559
BstACI GRCGYC 1 cut(s) 112
BstBAI YACGTR 1 cut(s) 588
BstC8I GCNNGC 1 cut(s) 593
BstDEI CTNAG 3 cut(s) 80, 260, 603
BstEII GGTNACC 1 cut(s) 607
BstF5I GGATG 2 cut(s) 68, 635
BstKTI GATC 2 cut(s) 379, 552
BstMBI GATC 2 cut(s) 376, 549
BstMWI GCNNNNNNNGC 2 cut(s) 196, 205
BstNSI RCATGY 2 cut(s) 595, 677
BstPI GGTNACC 1 cut(s) 607
BstSFI CTRYAG 2 cut(s) 197, 736
BstSLI GKGCMC 1 cut(s) 300
BstV1I GCAGC 2 cut(s) 268, 436
BsuRI GGCC 2 cut(s) 137, 157
BtsCI GGATG 2 cut(s) 68, 635
BtsIMutI CAGTG 2 cut(s) 165, 654
BveI ACCTGC 2 cut(s) 541, 729
Cac8I GCNNGC 1 cut(s) 593
Cfr13I GGNCC 1 cut(s) 364
Csp6I GTAC 3 cut(s) 144, 585, 655
CviAII CATG 3 cut(s) 374, 592, 674
CviQI GTAC 3 cut(s) 144, 585, 655
DdeI CTNAG 3 cut(s) 80, 260, 603
DpnI GATC 2 cut(s) 378, 551
DpnII GATC 2 cut(s) 376, 549
DraI TTTAAA 2 cut(s) 222, 456
DriI GACNNNNNGTC 1 cut(s) 490
Eam1104I CTCTTC 1 cut(s) 559
Eam1105I GACNNNNNGTC 1 cut(s) 490
EarI CTCTTC 1 cut(s) 559
Ecl136II GAGCTC 1 cut(s) 240
Eco24I GRGCYC 1 cut(s) 242
Eco47I GGWCC 1 cut(s) 364
Eco53kI GAGCTC 1 cut(s) 240
Eco57I CTGAAG 1 cut(s) 363
Eco91I GGTNACC 1 cut(s) 607
EcoICRI GAGCTC 1 cut(s) 240
EcoO65I GGTNACC 1 cut(s) 607
EcoRI GAATTC 1 cut(s) 17
EcoT38I GRGCYC 1 cut(s) 242
FaeI CATG 3 cut(s) 377, 595, 677
FalI AAGNNNNNCTT 4 cut(s) 336, 368, 341, 373
FatI CATG 3 cut(s) 373, 591, 673
FblI GTMKAC 2 cut(s) 115, 171
Fnu4HI GCNGC 2 cut(s) 257, 425
FokI GGATG 2 cut(s) 55, 642
FriOI GRGCYC 1 cut(s) 242
Fsp4HI GCNGC 2 cut(s) 257, 425
FspBI CTAG 1 cut(s) 416
GluI GCNGC 2 cut(s) 257, 425
HaeIII GGCC 2 cut(s) 137, 157
Hin1I GRCGYC 1 cut(s) 112
Hin1II CATG 3 cut(s) 377, 595, 677
HincII GTYRAC 1 cut(s) 116
HindII GTYRAC 1 cut(s) 116
HindIII AAGCTT 2 cut(s) 331, 350
HinfI GANTC 3 cut(s) 65, 93, 524
Hpy166II GTNNAC 7 cut(s) 116, 144, 172, 298, 308, 364, 655
Hpy188I TCNGA 3 cut(s) 343, 441, 568
Hpy8I GTNNAC 7 cut(s) 116, 144, 172, 298, 308, 364, 655
Hpy99I CGWCG 1 cut(s) 117
HpyAV CCTTC 2 cut(s) 7, 474
HpyCH4IV ACGT 3 cut(s) 57, 112, 587
HpyCH4V TGCA 7 cut(s) 199, 213, 298, 424, 591, 595, 738
HpyF10VI GCNNNNNNNGC 2 cut(s) 196, 205
HpyF3I CTNAG 3 cut(s) 80, 260, 603
HpySE526I ACGT 3 cut(s) 57, 112, 587
Hsp92I GRCGYC 1 cut(s) 112
Hsp92II CATG 3 cut(s) 377, 595, 677
Kzo9I GATC 2 cut(s) 376, 549
LmnI GCTCC 1 cut(s) 670
Lsp1109I GCAGC 2 cut(s) 268, 436
LweI GCATC 2 cut(s) 177, 673
MaeI CTAG 1 cut(s) 416
MaeII ACGT 3 cut(s) 57, 112, 587
MaeIII GTNAC 1 cut(s) 607
MalI GATC 2 cut(s) 378, 551
MboI GATC 2 cut(s) 376, 549
MboII GAAGA 3 cut(s) 290, 546, 704
MhlI GDGCHC 2 cut(s) 242, 300
MluCI AATT 3 cut(s) 17, 435, 457
MlyI GAGTC 1 cut(s) 87
MmeI TCCRAC 2 cut(s) 21, 42
MnlI CCTC 6 cut(s) 37, 83, 147, 198, 390, 574
MroXI GAANNNNTTC 2 cut(s) 17, 446
MseI TTAA 3 cut(s) 221, 455, 623
MslI CAYNNNNRTG 1 cut(s) 303
MspA1I CMGCKG 1 cut(s) 259
MwoI GCNNNNNNNGC 2 cut(s) 196, 205
NdeII GATC 2 cut(s) 376, 549
NlaIII CATG 3 cut(s) 377, 595, 677
NspI RCATGY 2 cut(s) 595, 677
OliI CACNNNNGTG 1 cut(s) 303
PaeI GCATGC 1 cut(s) 595
PdmI GAANNNNTTC 2 cut(s) 17, 446
PfeI GAWTC 2 cut(s) 65, 524
PkrI GCNGC 2 cut(s) 258, 426
PleI GAGTC 1 cut(s) 87
PpsI GAGTC 1 cut(s) 87
Ppu21I YACGTR 1 cut(s) 588
PsiI TTATAA 1 cut(s) 693
Psp124BI GAGCTC 1 cut(s) 242
PspEI GGTNACC 1 cut(s) 607
PspPI GGNCC 1 cut(s) 364
PstI CTGCAG 2 cut(s) 201, 740
PvuII CAGCTG 1 cut(s) 259
RsaI GTAC 3 cut(s) 145, 586, 656
RsaNI GTAC 3 cut(s) 144, 585, 655
RseI CAYNNNNRTG 1 cut(s) 303
SacI GAGCTC 1 cut(s) 242
SalI GTCGAC 1 cut(s) 114
SaqAI TTAA 3 cut(s) 221, 455, 623
SatI GCNGC 2 cut(s) 257, 425
Sau3AI GATC 2 cut(s) 376, 549
Sau96I GGNCC 1 cut(s) 364
SchI GAGTC 1 cut(s) 87
SduI GDGCHC 2 cut(s) 242, 300
SfaNI GCATC 2 cut(s) 177, 673
SfcI CTRYAG 2 cut(s) 197, 736
SinI GGWCC 1 cut(s) 364
SmiMI CAYNNNNRTG 1 cut(s) 303
SmlI CTYRAG 3 cut(s) 22, 95, 119
SmoI CTYRAG 3 cut(s) 22, 95, 119
SphI GCATGC 1 cut(s) 595
Sse9I AATT 3 cut(s) 17, 435, 457
SsiI CCGC 2 cut(s) 35, 401
SspMI CTAG 1 cut(s) 416
SstI GAGCTC 1 cut(s) 242
TaiI ACGT 3 cut(s) 60, 115, 590
TaqI TCGA 2 cut(s) 115, 232
TasI AATT 3 cut(s) 17, 435, 457
TatI WGTACW 2 cut(s) 143, 654
TfiI GAWTC 2 cut(s) 65, 524
Tru1I TTAA 3 cut(s) 221, 455, 623
Tru9I TTAA 3 cut(s) 221, 455, 623
TscAI CASTG 2 cut(s) 165, 654
TseI GCWGC 2 cut(s) 256, 424
TspDTI ATGAA 6 cut(s) 57, 491, 558, 704, 719, 753
TspRI CASTG 2 cut(s) 165, 654
VneI GTGCAC 1 cut(s) 296
VpaK11BI GGWCC 1 cut(s) 364
XapI RAATTY 2 cut(s) 17, 457
XceI RCATGY 2 cut(s) 595, 677
XmiI GTMKAC 2 cut(s) 115, 171
XmnI GAANNNNTTC 2 cut(s) 17, 446
XspI CTAG 1 cut(s) 416
ZraI GACGTC 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.