pycom04g03740

Domain of unknown function (DUF4504)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0012507
Physical Location & Seq
Reverse (-)
3340901 .. 3345581
4681 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g03740.1

Sequence Viewer

Length: 1023 bp
ATGCCAAACGGCCACTCAATGCTACTAGTAAACAAACAGTGGCATTTTCTGGAAGTCTGTCTTTTCTCTGTGTTAATGGAGGATCCTCGGGAAAAAGAAGACAAGGAATTCTTAACTGAAGCCGCAGAGTTAGATGAAGCATTGCGAGTCTTGGACTCCTCTCTCTCTCAAATCAAATGGCGTCTCAAATACTCTCGACGTCGACTTGACACAGATATTCTAGCTTTGTGCACAGGAATGAGGCCAGTGATACTGGTAGACTATGGTGGAAAGATGCCTGAACTGCAAGAGAGACTCTGCGCTATTTTGAAACTCTGTCAAAAGGAGTCACCCATTTTTGAGCATTTGAGAGTAATGGTTATAGAAGAAATGATATATTTGATACACGTTAGAGGGCTTGCTGATCATGTTAGGTCAAGCTTGAACTCAGAACGAGAGTTGCTCTTTGTGGATGTTGAACGAGATCCTCCAAAGATGATAACAGAAGCAGAAAAAAGCCCACTAGGGATGCAGCTTATATCTATTCAGAAGTTGTTCTCTCTGGTATTTCCTGTTGGTGGAAGGAAAGATGAGGCTTTGCCATCTCACAGTGTAACAGCTGATGCTGAACCGCCCATAAATGAACACATCCCTTCTCAGTCATCTGAATTTATCGATCTGAGTAGCTGCATGCAAAATACTGAGGTCTTACTGCCAACGCTAAATGGATGGCTTCTTGGGTATCCAGTTGTGTACTTGTTCAGAGAGGAGCATAGTGTTGCAGCTGTTTGTAATCTTTCTTCCAAGTATCTTCATATTTATAAAATATCAATATGCAGGAATGGATCCCCCGATGAAGTATATCAACTAGACGAACTCTTGAGTTTCTCAGTGCCCTACGATCTTAGCATGAGAGGGAGCAAGGAAAAATGGGCAGAGGCATTTTTGGCTCATATGCAGGGGAGATGGGAACGGTGCAAGCGTGCTTGGAGGCCTTTGCAGATGGAGGTTAGCGAACTAAAACCGCAAAGTATTGCGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4504

Protein Analysis

341

Amino Acids

39.14

Weight (kDa)

5.57

Isoelectric Point (pI)

55.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4504 PF14953 53 - 339 4.4e-83 Domain of unknown function (DUF4504)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012507)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59490 AT3G59490 AT3G59490 AT3G59490
fragaria_vesca FvH4_4g10850 FvH4_4g10850
malus_domestica MD04G1042500.v1.1
prunus_persica Prupe.1G196900_v2.0.a1
pyrus_communis pycom04g03740
rosa_chinensis RchiOBHm_Chr4g0406631
rosa_multiflora Rmu_sc0002774.1_g000005
rosa_roxburghii Rroxscaffold_5G00351470
rosa_rugosa Rorug04G0072400 Rorug04G0072500
rosa_samantha Rh4AG139700 Rh4BG136700 Rh4CG146900 Rh4DG133900
rosa_wichuraiana Rw4G011380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 801
AatII GACGTC 1 cut(s) 202
AccI GTMKAC 2 cut(s) 202, 258
AciI CCGC 3 cut(s) 123, 611, 1004
AclWI GGATC 5 cut(s) 77, 90, 458, 819, 832
AcoI YGGCCR 1 cut(s) 10
AcsI RAATTY 2 cut(s) 107, 647
AcuI CTGAAG 1 cut(s) 138
AcyI GRCGYC 2 cut(s) 181, 199
AfaI GTAC 1 cut(s) 734
AfiI CCNNNNNNNGG 1 cut(s) 557
AflIII ACRYGT 1 cut(s) 385
AgsI TTSAA 3 cut(s) 310, 424, 458
AhlI ACTAGT 1 cut(s) 25
AluBI AGCT 6 cut(s) 224, 420, 514, 599, 666, 764
AluI AGCT 6 cut(s) 224, 420, 514, 599, 666, 764
Alw21I GWGCWC 1 cut(s) 233
Alw26I GTCTC 2 cut(s) 188, 286
Alw44I GTGCAC 1 cut(s) 229
AlwI GGATC 5 cut(s) 77, 90, 458, 819, 832
Ama87I CYCGRG 1 cut(s) 87
AoxI GGCC 3 cut(s) 10, 242, 971
ApaLI GTGCAC 1 cut(s) 229
ApeKI GCWGC 3 cut(s) 511, 666, 761
ApoI RAATTY 2 cut(s) 107, 647
Asp700I GAANNNNTTC 1 cut(s) 533
AspLEI GCGC 1 cut(s) 302
AsuHPI GGTGA 1 cut(s) 321
AvaI CYCGRG 1 cut(s) 87
BaeGI GKGCMC 2 cut(s) 233, 876
BamHI GGATCC 2 cut(s) 82, 824
BarI GAAGNNNNNNTAC 2 cut(s) 763, 795
BbsI GAAGAC 1 cut(s) 105
Bbv12I GWGCWC 1 cut(s) 233
BbvI GCAGC 3 cut(s) 523, 653, 773
BccI CCATC 4 cut(s) 589, 702, 939, 976
BceAI ACGGC 1 cut(s) 25
BciVI GTATCC 1 cut(s) 732
BclI TGATCA 1 cut(s) 403
BcoDI GTCTC 2 cut(s) 188, 286
BcuI ACTAGT 1 cut(s) 25
BfaI CTAG 4 cut(s) 26, 221, 503, 848
BfuI GTATCC 1 cut(s) 732
BisI GCNGC 4 cut(s) 123, 512, 667, 762
BlsI GCNGC 4 cut(s) 124, 513, 668, 763
BmeT110I CYCGRG 1 cut(s) 87
BmiI GGNNCC 2 cut(s) 84, 826
BmsI GCATC 3 cut(s) 264, 498, 592
BpiI GAAGAC 1 cut(s) 105
BplI GAGNNNNNCTC 2 cut(s) 426, 458
BpuEI CTTGAG 1 cut(s) 880
Bsa29I ATCGAT 1 cut(s) 654
BsaHI GRCGYC 2 cut(s) 181, 199
BsaJI CCNNGG 1 cut(s) 86
Bsc4I CCNNNNNNNGG 1 cut(s) 557
Bse1I ACTGG 3 cut(s) 245, 258, 725
Bse3DI GCAATG 1 cut(s) 140
BseCI ATCGAT 1 cut(s) 654
BseDI CCNNGG 1 cut(s) 86
BseGI GGATG 4 cut(s) 457, 513, 627, 713
BseLI CCNNNNNNNGG 1 cut(s) 557
BseMI GCAATG 1 cut(s) 140
BseMII CTCAG 5 cut(s) 441, 650, 650, 672, 882
BseNI ACTGG 3 cut(s) 245, 258, 725
BseRI GAGGAG 2 cut(s) 148, 761
BseSI GKGCMC 2 cut(s) 233, 876
BseXI GCAGC 3 cut(s) 523, 653, 773
BshFI GGCC 3 cut(s) 12, 244, 973
BshVI ATCGAT 1 cut(s) 654
BsiHKAI GWGCWC 1 cut(s) 233
BsiHKCI CYCGRG 1 cut(s) 87
BslI CCNNNNNNNGG 1 cut(s) 557
BsmAI GTCTC 2 cut(s) 188, 286
BsmBI CGTCTC 1 cut(s) 188
BsnI GGCC 3 cut(s) 12, 244, 973
BsoBI CYCGRG 1 cut(s) 87
Bsp1286I GDGCHC 2 cut(s) 233, 876
Bsp143I GATC 6 cut(s) 82, 403, 463, 655, 824, 880
BspACI CCGC 3 cut(s) 123, 611, 1004
BspANI GGCC 3 cut(s) 12, 244, 973
BspCNI CTCAG 5 cut(s) 440, 649, 651, 673, 881
BspDI ATCGAT 1 cut(s) 654
BspLI GGNNCC 2 cut(s) 84, 826
BspPI GGATC 5 cut(s) 77, 90, 458, 819, 832
BsrDI GCAATG 1 cut(s) 140
BsrI ACTGG 3 cut(s) 245, 258, 725
BssECI CCNNGG 1 cut(s) 86
BssMI GATC 6 cut(s) 82, 403, 463, 655, 824, 880
BssNI GRCGYC 2 cut(s) 181, 199
Bst4CI ACNGT 3 cut(s) 39, 590, 954
BstACI GRCGYC 2 cut(s) 181, 199
BstC8I GCNNGC 4 cut(s) 399, 671, 959, 963
BstDEI CTNAG 6 cut(s) 427, 636, 659, 681, 868, 884
BstF5I GGATG 4 cut(s) 457, 513, 627, 713
BstHHI GCGC 1 cut(s) 302
BstKTI GATC 6 cut(s) 85, 406, 466, 658, 827, 883
BstMAI GTCTC 2 cut(s) 188, 286
BstMBI GATC 6 cut(s) 82, 403, 463, 655, 824, 880
BstMWI GCNNNNNNNGC 2 cut(s) 283, 926
BstNSI RCATGY 1 cut(s) 673
BstSLI GKGCMC 2 cut(s) 233, 876
BstV1I GCAGC 3 cut(s) 523, 653, 773
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 3 cut(s) 82, 463, 824
BstYI RGATCY 3 cut(s) 82, 463, 824
Bsu15I ATCGAT 1 cut(s) 654
BsuI GTATCC 1 cut(s) 732
BsuRI GGCC 3 cut(s) 12, 244, 973
BsuTUI ATCGAT 1 cut(s) 654
BtsCI GGATG 4 cut(s) 457, 513, 627, 713
BtsIMutI CAGTG 4 cut(s) 44, 252, 595, 876
Cac8I GCNNGC 4 cut(s) 399, 671, 959, 963
CfoI GCGC 1 cut(s) 302
ClaI ATCGAT 1 cut(s) 654
CseI GACGC 1 cut(s) 170
Csp6I GTAC 1 cut(s) 733
CviAII CATG 3 cut(s) 407, 670, 889
CviQI GTAC 1 cut(s) 733
DdeI CTNAG 6 cut(s) 427, 636, 659, 681, 868, 884
DpnI GATC 6 cut(s) 84, 405, 465, 657, 826, 882
DpnII GATC 6 cut(s) 82, 403, 463, 655, 824, 880
EaeI YGGCCR 1 cut(s) 10
Eco147I AGGCCT 1 cut(s) 973
Eco57I CTGAAG 1 cut(s) 138
Eco88I CYCGRG 1 cut(s) 87
EcoRI GAATTC 1 cut(s) 107
Esp3I CGTCTC 1 cut(s) 188
FaeI CATG 3 cut(s) 410, 673, 892
FalI AAGNNNNNCTT 4 cut(s) 45, 77, 95, 127
FatI CATG 3 cut(s) 406, 669, 888
FauNDI CATATG 1 cut(s) 933
FbaI TGATCA 1 cut(s) 403
FblI GTMKAC 2 cut(s) 202, 258
Fnu4HI GCNGC 4 cut(s) 123, 512, 667, 762
FokI GGATG 4 cut(s) 464, 520, 614, 720
Fsp4HI GCNGC 4 cut(s) 123, 512, 667, 762
FspBI CTAG 4 cut(s) 26, 221, 503, 848
GlaI GCGC 1 cut(s) 301
GluI GCNGC 4 cut(s) 123, 512, 667, 762
HaeIII GGCC 3 cut(s) 12, 244, 973
HgaI GACGC 1 cut(s) 170
HhaI GCGC 1 cut(s) 302
Hin1I GRCGYC 2 cut(s) 181, 199
Hin1II CATG 3 cut(s) 410, 673, 892
Hin6I GCGC 1 cut(s) 300
HinP1I GCGC 1 cut(s) 300
HincII GTYRAC 1 cut(s) 203
HindII GTYRAC 1 cut(s) 203
HindIII AAGCTT 1 cut(s) 418
HinfI GANTC 4 cut(s) 147, 155, 294, 326
HphI GGTGA 1 cut(s) 321
Hpy166II GTNNAC 5 cut(s) 31, 203, 231, 259, 733
Hpy188I TCNGA 5 cut(s) 430, 528, 646, 660, 743
Hpy188III TCNNGA 4 cut(s) 50, 89, 195, 859
Hpy8I GTNNAC 5 cut(s) 31, 203, 231, 259, 733
Hpy99I CGWCG 2 cut(s) 201, 204
HpyAV CCTTC 2 cut(s) 555, 642
HpyCH4III ACNGT 3 cut(s) 39, 590, 954
HpyCH4IV ACGT 2 cut(s) 199, 387
HpyF10VI GCNNNNNNNGC 2 cut(s) 283, 926
HpyF3I CTNAG 6 cut(s) 427, 636, 659, 681, 868, 884
HpySE526I ACGT 2 cut(s) 199, 387
Hsp92I GRCGYC 2 cut(s) 181, 199
Hsp92II CATG 3 cut(s) 410, 673, 892
HspAI GCGC 1 cut(s) 300
Ksp22I TGATCA 1 cut(s) 403
Kzo9I GATC 6 cut(s) 82, 403, 463, 655, 824, 880
LmnI GCTCC 2 cut(s) 748, 897
Lsp1109I GCAGC 3 cut(s) 523, 653, 773
LweI GCATC 3 cut(s) 264, 498, 592
MaeI CTAG 4 cut(s) 26, 221, 503, 848
MaeII ACGT 2 cut(s) 199, 387
MaeIII GTNAC 2 cut(s) 327, 592
MalI GATC 6 cut(s) 84, 405, 465, 657, 826, 882
MboI GATC 6 cut(s) 82, 403, 463, 655, 824, 880
MboII GAAGA 4 cut(s) 110, 377, 771, 782
MflI RGATCY 3 cut(s) 82, 463, 824
MhlI GDGCHC 2 cut(s) 233, 876
MluCI AATT 2 cut(s) 107, 647
MlyI GAGTC 4 cut(s) 149, 156, 288, 335
MroXI GAANNNNTTC 1 cut(s) 533
MseI TTAA 2 cut(s) 74, 113
MslI CAYNNNNRTG 1 cut(s) 236
MspA1I CMGCKG 2 cut(s) 599, 764
MwoI GCNNNNNNNGC 2 cut(s) 283, 926
NdeI CATATG 1 cut(s) 933
NdeII GATC 6 cut(s) 82, 403, 463, 655, 824, 880
NlaIII CATG 3 cut(s) 410, 673, 892
NlaIV GGNNCC 2 cut(s) 84, 826
NmuCI GTSAC 1 cut(s) 327
NspI RCATGY 1 cut(s) 673
PaeI GCATGC 1 cut(s) 673
PceI AGGCCT 1 cut(s) 973
PcsI WCGNNNNNNNCGW 1 cut(s) 958
PdmI GAANNNNTTC 1 cut(s) 533
PkrI GCNGC 4 cut(s) 124, 513, 668, 763
PleI GAGTC 4 cut(s) 149, 155, 288, 334
PpsI GAGTC 4 cut(s) 149, 155, 288, 334
PsiI TTATAA 1 cut(s) 801
PspN4I GGNNCC 2 cut(s) 84, 826
PsuI RGATCY 3 cut(s) 82, 463, 824
PvuII CAGCTG 2 cut(s) 599, 764
RsaI GTAC 1 cut(s) 734
RsaNI GTAC 1 cut(s) 733
RseI CAYNNNNRTG 1 cut(s) 236
SalI GTCGAC 1 cut(s) 201
SaqAI TTAA 2 cut(s) 74, 113
SatI GCNGC 4 cut(s) 123, 512, 667, 762
Sau3AI GATC 6 cut(s) 82, 403, 463, 655, 824, 880
SchI GAGTC 4 cut(s) 149, 156, 288, 335
SduI GDGCHC 2 cut(s) 233, 876
SfaNI GCATC 3 cut(s) 264, 498, 592
SmiMI CAYNNNNRTG 1 cut(s) 236
SmlI CTYRAG 1 cut(s) 859
SmoI CTYRAG 1 cut(s) 859
SpeI ACTAGT 1 cut(s) 25
SphI GCATGC 1 cut(s) 673
Sse9I AATT 2 cut(s) 107, 647
SseBI AGGCCT 1 cut(s) 973
SsiI CCGC 3 cut(s) 123, 611, 1004
SspMI CTAG 4 cut(s) 26, 221, 503, 848
StuI AGGCCT 1 cut(s) 973
TaaI ACNGT 3 cut(s) 39, 590, 954
TaiI ACGT 2 cut(s) 202, 390
TaqI TCGA 3 cut(s) 196, 202, 654
TasI AATT 2 cut(s) 107, 647
TatI WGTACW 1 cut(s) 732
TauI GCSGC 1 cut(s) 125
Tru1I TTAA 2 cut(s) 74, 113
Tru9I TTAA 2 cut(s) 74, 113
TscAI CASTG 4 cut(s) 44, 252, 595, 876
TseFI GTSAC 1 cut(s) 327
TseI GCWGC 3 cut(s) 511, 666, 761
Tsp45I GTSAC 1 cut(s) 327
TspDTI ATGAA 4 cut(s) 150, 636, 782, 849
TspRI CASTG 4 cut(s) 44, 252, 595, 876
VneI GTGCAC 1 cut(s) 229
XapI RAATTY 2 cut(s) 107, 647
XceI RCATGY 1 cut(s) 673
XmiI GTMKAC 2 cut(s) 202, 258
XmnI GAANNNNTTC 1 cut(s) 533
XspI CTAG 4 cut(s) 26, 221, 503, 848
ZraI GACGTC 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.