MD04G1042500.v1.1

Domain of unknown function (DUF4504)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0012507
Physical Location & Seq
Reverse (-)
5009499 .. 5015051
5553 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1042500.v1.1.491

Sequence Viewer

Length: 948 bp
ATGGAGGATCCTCGAGAAAAAGAAGACAAGGAATTCTTAACCGAAGCTGCAGAGTTAGATGAAGCATTGAGAGTGTTGAACTCCTCTCTCTCTCAAATCAAATGGCGTCTCAAATACTCTCGACGTCGACTTGACACAGATATTCTAGCTTTGTGCACTGGAATGAGGCCAGTGATACTGGTAGACTATGGCGGAAAGATGCCTGAACTGCAAGAGAGACTCTGCGCTATTTTGAAACTTTGTCAAAAGGAGTCACCCATTTTTGAGCATTTGAGAGTAATGGTTATAGAAGAAATGATATATTTGATACACGTTAGAGGGCTTGCTGATCATGTTAGGTCAAGCTTGAACTCAGAACAAGAGTTGCTCTTTGTGGATGTTGAACGAGATCCTCCAAAGATGATAACAGAAGCAGAAAAAAACCCATTGGGGATGCAGCTTATATCTATTCAGAAGTTGTTCTCTCTGGTATTTCCTGTTGGTGGAAGGAAAGATGAGGCTTCGCCATCTCACAGTATAACAGCTGATGCTGAACCGCCCATAAATGAACTCATCCCTTCTCAGTCATCTGAATTTATCGATCTGAGTAGCTGCATGCAAAATACTGAGGTCTTACTGCCAACGCTAAATGGATGGCTTCTTGGGTATCCAGTTGTGTACTTGTTCAGAGAGGAGCATAATGTTGCTGCTGTTTGTAATCTTTCTTCCAAGTGTCTTCATATCTATAAAATATCAATATGCAGGAATGGATCCCCTGATGAAGTATATCAACCAGACGAACTCTTGAGTTTCTCGGTGCCCTACGATCTTAGCATGAGAGGGAGCAAGGAAAAATGGGCAGAGGCATTTTTGGCTCATATGCAGGGGAGATGGGAAAGGTGCGAGCGTGCTTGGAGGCCTTTGCAGATGGAGGTTAGCGAACTAAAACCGCAAACTATTGCGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4504

Protein Analysis

316

Amino Acids

36.14

Weight (kDa)

5.2

Isoelectric Point (pI)

54.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4504 PF14953 28 - 314 2.5e-82 Domain of unknown function (DUF4504)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012507)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59490 AT3G59490 AT3G59490 AT3G59490
fragaria_vesca FvH4_4g10850 FvH4_4g10850
malus_domestica MD04G1042500.v1.1
prunus_persica Prupe.1G196900_v2.0.a1
pyrus_communis pycom04g03740
rosa_chinensis RchiOBHm_Chr4g0406631
rosa_multiflora Rmu_sc0002774.1_g000005
rosa_roxburghii Rroxscaffold_5G00351470
rosa_rugosa Rorug04G0072400 Rorug04G0072500
rosa_samantha Rh4AG139700 Rh4BG136700 Rh4CG146900 Rh4DG133900
rosa_wichuraiana Rw4G011380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 127
AccB1I GGYRCC 1 cut(s) 796
AccI GTMKAC 2 cut(s) 127, 183
AciI CCGC 3 cut(s) 192, 536, 929
AclWI GGATC 5 cut(s) 2, 15, 383, 744, 757
AcsI RAATTY 2 cut(s) 32, 572
AcyI GRCGYC 2 cut(s) 106, 124
AfaI GTAC 1 cut(s) 659
AfiI CCNNNNNNNGG 1 cut(s) 482
AflIII ACRYGT 1 cut(s) 310
AgsI TTSAA 4 cut(s) 79, 235, 349, 383
AluBI AGCT 6 cut(s) 47, 149, 345, 439, 524, 591
AluI AGCT 6 cut(s) 47, 149, 345, 439, 524, 591
Alw21I GWGCWC 1 cut(s) 158
Alw26I GTCTC 2 cut(s) 113, 211
Alw44I GTGCAC 1 cut(s) 154
AlwI GGATC 5 cut(s) 2, 15, 383, 744, 757
Ama87I CYCGRG 1 cut(s) 12
AoxI GGCC 2 cut(s) 167, 896
ApaLI GTGCAC 1 cut(s) 154
ApeKI GCWGC 4 cut(s) 47, 436, 591, 686
ApoI RAATTY 2 cut(s) 32, 572
Asp700I GAANNNNTTC 1 cut(s) 458
AspLEI GCGC 1 cut(s) 227
AsuHPI GGTGA 1 cut(s) 246
AvaI CYCGRG 1 cut(s) 12
BaeGI GKGCMC 2 cut(s) 158, 801
BamHI GGATCC 2 cut(s) 7, 749
BanI GGYRCC 1 cut(s) 796
BarI GAAGNNNNNNTAC 2 cut(s) 688, 720
BbsI GAAGAC 2 cut(s) 30, 707
Bbv12I GWGCWC 1 cut(s) 158
BbvI GCAGC 4 cut(s) 34, 448, 578, 673
BccI CCATC 4 cut(s) 514, 627, 864, 901
BciVI GTATCC 1 cut(s) 657
BclI TGATCA 1 cut(s) 328
BcoDI GTCTC 2 cut(s) 113, 211
BfaI CTAG 1 cut(s) 146
BfmI CTRYAG 1 cut(s) 48
BfuI GTATCC 1 cut(s) 657
BisI GCNGC 4 cut(s) 48, 437, 592, 687
BlsI GCNGC 4 cut(s) 49, 438, 593, 688
BmeT110I CYCGRG 1 cut(s) 12
BmiI GGNNCC 3 cut(s) 9, 751, 798
BmsI GCATC 3 cut(s) 189, 423, 517
BpiI GAAGAC 2 cut(s) 30, 707
BpuEI CTTGAG 1 cut(s) 805
Bsa29I ATCGAT 1 cut(s) 579
BsaHI GRCGYC 2 cut(s) 106, 124
Bsc4I CCNNNNNNNGG 1 cut(s) 482
Bse1I ACTGG 4 cut(s) 163, 170, 183, 650
BseCI ATCGAT 1 cut(s) 579
BseGI GGATG 4 cut(s) 382, 438, 552, 638
BseLI CCNNNNNNNGG 1 cut(s) 482
BseMII CTCAG 4 cut(s) 366, 575, 575, 597
BseNI ACTGG 4 cut(s) 163, 170, 183, 650
BseRI GAGGAG 2 cut(s) 73, 686
BseSI GKGCMC 2 cut(s) 158, 801
BseXI GCAGC 4 cut(s) 34, 448, 578, 673
BshFI GGCC 2 cut(s) 169, 898
BshNI GGYRCC 1 cut(s) 796
BshVI ATCGAT 1 cut(s) 579
BsiHKAI GWGCWC 1 cut(s) 158
BsiHKCI CYCGRG 1 cut(s) 12
BslI CCNNNNNNNGG 1 cut(s) 482
BsmAI GTCTC 2 cut(s) 113, 211
BsmBI CGTCTC 1 cut(s) 113
BsnI GGCC 2 cut(s) 169, 898
BsoBI CYCGRG 1 cut(s) 12
Bsp1286I GDGCHC 2 cut(s) 158, 801
Bsp143I GATC 6 cut(s) 7, 328, 388, 580, 749, 805
BspACI CCGC 3 cut(s) 192, 536, 929
BspANI GGCC 2 cut(s) 169, 898
BspCNI CTCAG 4 cut(s) 365, 574, 576, 598
BspDI ATCGAT 1 cut(s) 579
BspLI GGNNCC 3 cut(s) 9, 751, 798
BspMAI CTGCAG 1 cut(s) 52
BspPI GGATC 5 cut(s) 2, 15, 383, 744, 757
BspT107I GGYRCC 1 cut(s) 796
BsrI ACTGG 4 cut(s) 163, 170, 183, 650
BssMI GATC 6 cut(s) 7, 328, 388, 580, 749, 805
BssNI GRCGYC 2 cut(s) 106, 124
Bst4CI ACNGT 1 cut(s) 515
BstACI GRCGYC 2 cut(s) 106, 124
BstC8I GCNNGC 4 cut(s) 324, 596, 884, 888
BstDEI CTNAG 5 cut(s) 352, 561, 584, 606, 809
BstF5I GGATG 4 cut(s) 382, 438, 552, 638
BstHHI GCGC 1 cut(s) 227
BstKTI GATC 6 cut(s) 10, 331, 391, 583, 752, 808
BstMAI GTCTC 2 cut(s) 113, 211
BstMBI GATC 6 cut(s) 7, 328, 388, 580, 749, 805
BstMWI GCNNNNNNNGC 2 cut(s) 208, 851
BstNSI RCATGY 1 cut(s) 598
BstSFI CTRYAG 1 cut(s) 48
BstSLI GKGCMC 2 cut(s) 158, 801
BstV1I GCAGC 4 cut(s) 34, 448, 578, 673
BstV2I GAAGAC 2 cut(s) 30, 707
BstX2I RGATCY 3 cut(s) 7, 388, 749
BstYI RGATCY 3 cut(s) 7, 388, 749
Bsu15I ATCGAT 1 cut(s) 579
BsuI GTATCC 1 cut(s) 657
BsuRI GGCC 2 cut(s) 169, 898
BsuTUI ATCGAT 1 cut(s) 579
BtsCI GGATG 4 cut(s) 382, 438, 552, 638
BtsIMutI CAGTG 2 cut(s) 156, 177
Cac8I GCNNGC 4 cut(s) 324, 596, 884, 888
CfoI GCGC 1 cut(s) 227
ClaI ATCGAT 1 cut(s) 579
CseI GACGC 1 cut(s) 95
Csp6I GTAC 1 cut(s) 658
CviAII CATG 3 cut(s) 332, 595, 814
CviQI GTAC 1 cut(s) 658
DdeI CTNAG 5 cut(s) 352, 561, 584, 606, 809
DpnI GATC 6 cut(s) 9, 330, 390, 582, 751, 807
DpnII GATC 6 cut(s) 7, 328, 388, 580, 749, 805
EciI GGCGGA 1 cut(s) 207
Eco147I AGGCCT 1 cut(s) 898
Eco88I CYCGRG 1 cut(s) 12
EcoRI GAATTC 1 cut(s) 32
Esp3I CGTCTC 1 cut(s) 113
FaeI CATG 3 cut(s) 335, 598, 817
FalI AAGNNNNNCTT 2 cut(s) 20, 52
FatI CATG 3 cut(s) 331, 594, 813
FauNDI CATATG 1 cut(s) 858
FbaI TGATCA 1 cut(s) 328
FblI GTMKAC 2 cut(s) 127, 183
Fnu4HI GCNGC 4 cut(s) 48, 437, 592, 687
FokI GGATG 4 cut(s) 389, 445, 539, 645
Fsp4HI GCNGC 4 cut(s) 48, 437, 592, 687
FspBI CTAG 1 cut(s) 146
GlaI GCGC 1 cut(s) 226
GluI GCNGC 4 cut(s) 48, 437, 592, 687
HaeIII GGCC 2 cut(s) 169, 898
HgaI GACGC 1 cut(s) 95
HhaI GCGC 1 cut(s) 227
Hin1I GRCGYC 2 cut(s) 106, 124
Hin1II CATG 3 cut(s) 335, 598, 817
Hin6I GCGC 1 cut(s) 225
HinP1I GCGC 1 cut(s) 225
HincII GTYRAC 1 cut(s) 128
HindII GTYRAC 1 cut(s) 128
HindIII AAGCTT 1 cut(s) 343
HinfI GANTC 2 cut(s) 219, 251
HphI GGTGA 1 cut(s) 246
Hpy166II GTNNAC 4 cut(s) 128, 156, 184, 658
Hpy188I TCNGA 5 cut(s) 355, 453, 571, 585, 668
Hpy188III TCNNGA 3 cut(s) 14, 120, 784
Hpy8I GTNNAC 4 cut(s) 128, 156, 184, 658
Hpy99I CGWCG 2 cut(s) 126, 129
HpyAV CCTTC 2 cut(s) 480, 567
HpyCH4III ACNGT 1 cut(s) 515
HpyCH4IV ACGT 2 cut(s) 124, 312
HpyCH4V TGCA 9 cut(s) 50, 156, 211, 436, 594, 598, 741, 862, 904
HpyF10VI GCNNNNNNNGC 2 cut(s) 208, 851
HpyF3I CTNAG 5 cut(s) 352, 561, 584, 606, 809
HpySE526I ACGT 2 cut(s) 124, 312
Hsp92I GRCGYC 2 cut(s) 106, 124
Hsp92II CATG 3 cut(s) 335, 598, 817
HspAI GCGC 1 cut(s) 225
Ksp22I TGATCA 1 cut(s) 328
Kzo9I GATC 6 cut(s) 7, 328, 388, 580, 749, 805
LmnI GCTCC 2 cut(s) 673, 822
Lsp1109I GCAGC 4 cut(s) 34, 448, 578, 673
LweI GCATC 3 cut(s) 189, 423, 517
MaeI CTAG 1 cut(s) 146
MaeII ACGT 2 cut(s) 124, 312
MaeIII GTNAC 1 cut(s) 252
MalI GATC 6 cut(s) 9, 330, 390, 582, 751, 807
MboI GATC 6 cut(s) 7, 328, 388, 580, 749, 805
MboII GAAGA 4 cut(s) 35, 302, 696, 707
MflI RGATCY 3 cut(s) 7, 388, 749
MhlI GDGCHC 2 cut(s) 158, 801
MluCI AATT 2 cut(s) 32, 572
MlyI GAGTC 2 cut(s) 213, 260
MroXI GAANNNNTTC 1 cut(s) 458
MseI TTAA 1 cut(s) 38
MslI CAYNNNNRTG 1 cut(s) 161
MspA1I CMGCKG 1 cut(s) 524
MwoI GCNNNNNNNGC 2 cut(s) 208, 851
NdeI CATATG 1 cut(s) 858
NdeII GATC 6 cut(s) 7, 328, 388, 580, 749, 805
NlaIII CATG 3 cut(s) 335, 598, 817
NlaIV GGNNCC 3 cut(s) 9, 751, 798
NmuCI GTSAC 1 cut(s) 252
NspI RCATGY 1 cut(s) 598
PaeI GCATGC 1 cut(s) 598
PaeR7I CTCGAG 1 cut(s) 12
PceI AGGCCT 1 cut(s) 898
PdmI GAANNNNTTC 1 cut(s) 458
PkrI GCNGC 4 cut(s) 49, 438, 593, 688
PleI GAGTC 2 cut(s) 213, 259
PpsI GAGTC 2 cut(s) 213, 259
PspN4I GGNNCC 3 cut(s) 9, 751, 798
PstI CTGCAG 1 cut(s) 52
PsuI RGATCY 3 cut(s) 7, 388, 749
PvuII CAGCTG 1 cut(s) 524
RsaI GTAC 1 cut(s) 659
RsaNI GTAC 1 cut(s) 658
RseI CAYNNNNRTG 1 cut(s) 161
SalI GTCGAC 1 cut(s) 126
SaqAI TTAA 1 cut(s) 38
SatI GCNGC 4 cut(s) 48, 437, 592, 687
Sau3AI GATC 6 cut(s) 7, 328, 388, 580, 749, 805
SchI GAGTC 2 cut(s) 213, 260
SduI GDGCHC 2 cut(s) 158, 801
SfaNI GCATC 3 cut(s) 189, 423, 517
SfcI CTRYAG 1 cut(s) 48
Sfr274I CTCGAG 1 cut(s) 12
SlaI CTCGAG 1 cut(s) 12
SmiMI CAYNNNNRTG 1 cut(s) 161
SmlI CTYRAG 2 cut(s) 12, 784
SmoI CTYRAG 2 cut(s) 12, 784
SphI GCATGC 1 cut(s) 598
Sse9I AATT 2 cut(s) 32, 572
SseBI AGGCCT 1 cut(s) 898
SsiI CCGC 3 cut(s) 192, 536, 929
SspMI CTAG 1 cut(s) 146
StuI AGGCCT 1 cut(s) 898
TaaI ACNGT 1 cut(s) 515
TaiI ACGT 2 cut(s) 127, 315
TaqI TCGA 4 cut(s) 13, 121, 127, 579
TasI AATT 2 cut(s) 32, 572
TatI WGTACW 1 cut(s) 657
Tru1I TTAA 1 cut(s) 38
Tru9I TTAA 1 cut(s) 38
TscAI CASTG 2 cut(s) 163, 177
TseFI GTSAC 1 cut(s) 252
TseI GCWGC 4 cut(s) 47, 436, 591, 686
Tsp45I GTSAC 1 cut(s) 252
TspDTI ATGAA 4 cut(s) 75, 561, 707, 774
TspRI CASTG 2 cut(s) 163, 177
VneI GTGCAC 1 cut(s) 154
XapI RAATTY 2 cut(s) 32, 572
XceI RCATGY 1 cut(s) 598
XhoI CTCGAG 1 cut(s) 12
XmiI GTMKAC 2 cut(s) 127, 183
XmnI GAANNNNTTC 1 cut(s) 458
XspI CTAG 1 cut(s) 146
ZraI GACGTC 1 cut(s) 125
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.