Rorug04G0072500

Domain of unknown function (DUF4504)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0012507
Physical Location & Seq
Forward (+)
11200166 .. 11205759
5594 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0072500.1

Sequence Viewer

Length: 2439 bp
ATGAAAGTGGCAGTGATTGGTTCTGGTATTAGTGGTTTGGTCTCAGCCTATGTTCTTGCAAAAGAAGGCGTTGAAGTGGTTCTATATGAGAAAAAAGACTACCTGGGTGGCCATGCTAGGACTGTCACATGTGATGGTGTTGATTTGGACCTTGGTTTCATGGTCTTCAATCATGGAAGTACACTGGTGATGTATCCAAATATGATGGAGTTCTTTGAGAGCCTTGGAATTGATATGGAGACATGCGACATGTCCTTCTCAGTGAGTTTAGATAAAGGACGGGGCTGTGAATGGGGTAGTCGAAATAGTCTCTCAAGCTTGTTTGCAGAAAAGAAGAACTTGTTCAACCCATACTTCTGGAAAATGCTTCAAGAAATCACCAAGTTTAAGCATGATTTGATCAGTTATCTTGAGGTGCTTGAGAGTAACCCAGACATTGTCCGGAATGAAACCTTGGGGCAGTTTATTGAGTCACATCACTACTCTGAACTATTTCGGAAGGCTTACCTTATTCCAATATGCAGTTCAATCTTGTCGTGTCCTTCAGAAGGAGTTATAAGCTTGTCAGCTTTCTCTGTGCTTTCGGTTTGTCGCAATTATCAACTACTTCAGCTATTTGGCGGCCCACAGTGGCTTACCGTCAAATGGCGTTCACACTGTTATGTGAAGAAGGTGAAAGAAGTGTTGGAGAGTAAGGGTTGTCAGATAAGAACCGGCTGTGAGGTACATAAAGTTTCAACAACTGATGAGGGTTGCACTGTTTTTACTGGAGATGGTTTTGAGGAAATTTACAATGGATGCATTATTGCTATCCAAGCCCCCAAAGCCGTGAGAATATTAGGAGATCAGGCAACGCCTGATGAACTGAGAATACTAGGCGCCTTCCAATATGTGTACTGTGATATTTTCCTTCATCGTGACAAAACTTTAATGCCCCAAAACCTAGCAGCATGGAGTGCATTTAACTATCTTGGAAGTAGTGACAACAAAGTATGTTTGACATACTGGCTCAATCAGCTTCAGAACCTCGGTGAAACAAGTCAACCATATCTTGTGACACTCAATCCAGATCATACACCAAACCACACCTTGCTTAAGTGGTCAACAAGCCATCCAGTCCTATCTGTTGCAGCATCAAAAGCTTCGCTGGAGCTTCCTCATATTCAAGGAAAGAGAGGAATTTGGTTTTGTGGAGCATACCAGGGTTATGGCTTCCATGAGGATGGGCTAAAGGCTGGAATGGCTGCAGCCCATGGTATGCTTGGAAAAGGTTGTGCCCTGTTGGGAAACCCAAAATATATGTCTTTTTCAACGACGGAAACAGCGGCACGCCTGTTTGTTACTAGATTTCTTAGACATTATATATCTACCGGATGTTTGATCTTATTAGAAGAGGGAGGCACAATGTTCACCTTTGAGGGAAGCAGAAAGGACTGTTCTCTAAAATCCGTTCTCAAAGTTCACAGTCCTCAGTTTTACTGGAAAGTAATGACAAAGGCTGATTTAGGCATTGCAGATGCATACATCAATGGTGATTTTTCTTTCAATGATAAAGACCAAGGTCTTCTAAATCTTATTATGCTTCTCATTGCCAACGGAGATTCTAATGCCTCAAAATCAAACAAGAAAAGGGGAATGTGGCAGCCTTTGTTTTTCACAGCCACTATAGGCTCTATAGCCTCAGCCAGATACTTCTTTCAGCATGCTTTGAAGAAAAATAGTCTTGCACAGGCTCGAAGGAATATATCTCATCATTATGATTTGAGTAATGAACTCTTTTCGCTGTTCTTGGGCGAAACAATGATGTATTCCGCTGCAATATTTAAGACAGAAGATGAGGAACTGAATACTGCACAGCTTAGGAAAATCTCTATTCTCATTGATAAGGCTAGAATCGATGAGAACCATGAGGTTCTAGATATGGGTTGTGGTTGGGGAGCCATAGCTATTGAAGCAGTCAAACAAACGGGCTGCAGATACACTGGCATCACTCTATCTGAGGAACAACTTAAATTTGCTGAAAAGAAAGTGAAAGATGCTGGACTTCAGCACCGGATCAAGTTCCTTTTGTGTGACTACCGCCAATTGCCTTCTTACCACAAATACGACAGAATCATAGCTTGCGAGATCTTAGAACATGTCGGCCATGAATTTGTGGATGAGTTTTTTTCATGCTGTGAGTCTTTACTAGCAGAAAATGGGATTCTTGTTCAGCAGTTCACATCTATAGCGGATGAACGCTATGATGAGTTCAGGCGAAGTTCAGGCTTCATAAAGGAATATATATTTCCTGGTACATGTCTACTTTCATTAAGTAGGGTAACATCAGCCATGGCCGCTTCTTCTAGACTTTGTGTGGAGCACTTGGAAAACATCGGAATTCATTATTACCAGACACTACGATGTTGGAGAAAGAATTTTTTAGAGAGACAGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4504

Protein Analysis

812

Amino Acids

91.61

Weight (kDa)

6.7

Isoelectric Point (pI)

35.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DAO PF01266 2 - 39 3.9e-07 FAD dependent oxidoreductase
NAD_binding_8 PF13450 5 - 74 1.6e-15 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 10 - 280 6.6e-17 Flavin containing amine oxidoreductase
CMAS PF02353 576 - 810 2.9e-66 Mycolic acid cyclopropane synthetase
MTS PF05175 627 - 736 2.4e-06 Methyltransferase small domain
Methyltransf_23 PF13489 630 - 738 7.7e-08 Methyltransferase domain
PrmA PF06325 631 - 751 8.9e-07 Ribosomal protein L11 methyltransferase (PrmA)
Methyltransf_31 PF13847 633 - 740 2.6e-12 Methyltransferase domain
Methyltransf_25 PF13649 638 - 734 1.2e-15 Methyltransferase domain
Methyltransf_11 PF08241 639 - 737 1.4e-12 Methyltransferase domain
Methyltransf_12 PF08242 639 - 736 4e-12 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012507)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59490 AT3G59490 AT3G59490 AT3G59490
fragaria_vesca FvH4_4g10850 FvH4_4g10850
malus_domestica MD04G1042500.v1.1
prunus_persica Prupe.1G196900_v2.0.a1
pyrus_communis pycom04g03740
rosa_chinensis RchiOBHm_Chr4g0406631
rosa_multiflora Rmu_sc0002774.1_g000005
rosa_roxburghii Rroxscaffold_5G00351470
rosa_rugosa Rorug04G0072400 Rorug04G0072500
rosa_samantha Rh4AG139700 Rh4BG136700 Rh4CG146900 Rh4DG133900
rosa_wichuraiana Rw4G011380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 557
AccB1I GGYRCC 1 cut(s) 878
AccI GTMKAC 1 cut(s) 2302
AccIII TCCGGA 1 cut(s) 441
AciI CCGC 6 cut(s) 621, 1325, 1812, 2080, 2231, 2337
AclWI GGATC 1 cut(s) 2063
AcoI YGGCCR 3 cut(s) 109, 2143, 2334
AcsI RAATTY 6 cut(s) 786, 1179, 2012, 2150, 2379, 2416
AcuI CTGAAG 4 cut(s) 528, 593, 1004, 2030
AcyI GRCGYC 1 cut(s) 879
AfaI GTAC 4 cut(s) 181, 726, 896, 2296
AfiI CCNNNNNNNGG 2 cut(s) 548, 645
AflII CTTAAG 1 cut(s) 1094
AflIII ACRYGT 4 cut(s) 128, 249, 2137, 2297
AjnI CCWGG 3 cut(s) 102, 1200, 2290
AjuI GAANNNNNNNTTGG 6 cut(s) 437, 469, 508, 540, 668, 700
Alw21I GWGCWC 1 cut(s) 2364
Alw26I GTCTC 4 cut(s) 46, 233, 314, 2422
AlwI GGATC 1 cut(s) 2063
Aor13HI TCCGGA 1 cut(s) 441
AoxI GGCC 4 cut(s) 109, 622, 2143, 2334
ApeKI GCWGC 7 cut(s) 947, 1130, 1244, 1247, 1642, 1814, 1971
ApoI RAATTY 6 cut(s) 786, 1179, 2012, 2150, 2379, 2416
ArsI GACNNNNNNTTYG 2 cut(s) 1286, 1318
Asp700I GAANNNNTTC 3 cut(s) 78, 341, 492
AspLEI GCGC 1 cut(s) 881
AspS9I GGNCC 2 cut(s) 148, 623
AsuHPI GGTGA 6 cut(s) 199, 370, 685, 1043, 1402, 1544
AvaII GGWCC 1 cut(s) 148
BaeGI GKGCMC 1 cut(s) 1279
BalI TGGCCA 1 cut(s) 111
BanI GGYRCC 1 cut(s) 878
BbsI GAAGAC 2 cut(s) 157, 1556
Bbv12I GWGCWC 1 cut(s) 2364
BbvCI CCTCAGC 1 cut(s) 1681
BbvI GCAGC 7 cut(s) 959, 1142, 1231, 1259, 1654, 1801, 1958
BccI CCATC 5 cut(s) 128, 199, 767, 1119, 1217
BceAI ACGGC 1 cut(s) 812
BciT130I CCWGG 3 cut(s) 104, 1202, 2292
BciVI GTATCC 1 cut(s) 204
BclI TGATCA 1 cut(s) 399
BcoDI GTCTC 4 cut(s) 46, 233, 314, 2422
BfaI CTAG 8 cut(s) 117, 875, 944, 1344, 1890, 1916, 2189, 2346
BfmI CTRYAG 5 cut(s) 1245, 1665, 1674, 1972, 2226
BfoI RGCGCY 1 cut(s) 882
BfrI CTTAAG 1 cut(s) 1094
BfuI GTATCC 1 cut(s) 204
BglII AGATCT 1 cut(s) 2127
Bme1390I CCNGG 3 cut(s) 104, 1202, 2292
Bme18I GGWCC 1 cut(s) 148
BmgT120I GGNCC 2 cut(s) 148, 623
BmiI GGNNCC 2 cut(s) 880, 1939
BmrFI CCNGG 3 cut(s) 104, 1202, 2292
BmsI GCATC 5 cut(s) 788, 1142, 1507, 1995, 2026
BoxI GACNNNNGTC 1 cut(s) 1560
BpiI GAAGAC 2 cut(s) 157, 1556
BpmI CTGGAG 2 cut(s) 789, 1169
Bpu10I CCTNAGC 2 cut(s) 1681, 1859
BpuEI CTTGAG 3 cut(s) 298, 431, 440
Bsa29I ATCGAT 1 cut(s) 1896
BsaHI GRCGYC 1 cut(s) 879
BsaI GGTCTC 1 cut(s) 46
BsaJI CCNNGG 9 cut(s) 103, 151, 223, 453, 1027, 1201, 1252, 1558, 2331
BsaWI WCCGGW 3 cut(s) 441, 1370, 2052
Bsc4I CCNNNNNNNGG 2 cut(s) 548, 645
Bse118I RCCGGY 1 cut(s) 713
Bse1I ACTGG 6 cut(s) 189, 772, 1010, 1115, 1484, 1987
Bse3DI GCAATG 2 cut(s) 1509, 1587
BseAI TCCGGA 1 cut(s) 441
BseBI CCWGG 3 cut(s) 104, 1202, 2292
BseCI ATCGAT 1 cut(s) 1896
BseDI CCNNGG 9 cut(s) 103, 151, 223, 453, 1027, 1201, 1252, 1558, 2331
BseGI GGATG 6 cut(s) 803, 1111, 1228, 1379, 2164, 2239
BseLI CCNNNNNNNGG 2 cut(s) 548, 645
BseMI GCAATG 2 cut(s) 1509, 1587
BseMII CTCAG 6 cut(s) 57, 273, 857, 1484, 1695, 1989
BseNI ACTGG 6 cut(s) 189, 772, 1010, 1115, 1484, 1987
BseSI GKGCMC 1 cut(s) 1279
BseXI GCAGC 7 cut(s) 959, 1142, 1231, 1259, 1654, 1801, 1958
BsgI GTGCAG 1 cut(s) 1836
BshFI GGCC 4 cut(s) 111, 624, 2145, 2336
BshNI GGYRCC 1 cut(s) 878
BshVI ATCGAT 1 cut(s) 1896
BsiHKAI GWGCWC 1 cut(s) 2364
BsiSI CCGG 4 cut(s) 442, 714, 1371, 2053
BslI CCNNNNNNNGG 2 cut(s) 548, 645
BsmAI GTCTC 4 cut(s) 46, 233, 314, 2422
BsnI GGCC 4 cut(s) 111, 624, 2145, 2336
Bso31I GGTCTC 1 cut(s) 46
Bsp1286I GDGCHC 2 cut(s) 1279, 2364
Bsp13I TCCGGA 1 cut(s) 441
Bsp143I GATC 6 cut(s) 399, 844, 1069, 1380, 2055, 2127
Bsp19I CCATGG 2 cut(s) 1252, 2331
BspACI CCGC 6 cut(s) 621, 1325, 1812, 2080, 2231, 2337
BspANI GGCC 4 cut(s) 111, 624, 2145, 2336
BspCNI CTCAG 6 cut(s) 56, 272, 858, 1483, 1694, 1990
BspDI ATCGAT 1 cut(s) 1896
BspEI TCCGGA 1 cut(s) 441
BspLI GGNNCC 2 cut(s) 880, 1939
BspMAI CTGCAG 2 cut(s) 1249, 1976
BspPI GGATC 1 cut(s) 2063
BspT107I GGYRCC 1 cut(s) 878
BspTI CTTAAG 1 cut(s) 1094
BspTNI GGTCTC 1 cut(s) 46
BsrDI GCAATG 2 cut(s) 1509, 1587
BsrFI RCCGGY 1 cut(s) 713
BsrI ACTGG 6 cut(s) 189, 772, 1010, 1115, 1484, 1987
BssAI RCCGGY 1 cut(s) 713
BssECI CCNNGG 9 cut(s) 103, 151, 223, 453, 1027, 1201, 1252, 1558, 2331
BssMI GATC 6 cut(s) 399, 844, 1069, 1380, 2055, 2127
BssNI GRCGYC 1 cut(s) 879
BssT1I CCWWGG 6 cut(s) 151, 223, 453, 1252, 1558, 2331
Bst2UI CCWGG 3 cut(s) 104, 1202, 2292
Bst4CI ACNGT 8 cut(s) 124, 630, 640, 659, 760, 899, 1436, 1466
Bst6I CTCTTC 1 cut(s) 1386
BstACI GRCGYC 1 cut(s) 879
BstAFI CTTAAG 1 cut(s) 1094
BstAPI GCANNNNNTGC 1 cut(s) 956
BstC8I GCNNGC 3 cut(s) 1330, 1704, 2122
BstDEI CTNAG 9 cut(s) 43, 259, 866, 1352, 1470, 1681, 1859, 1998, 2131
BstDSI CCRYGG 2 cut(s) 1252, 2331
BstENI CCTNNNNNAGG 1 cut(s) 546
BstF5I GGATG 6 cut(s) 803, 1111, 1228, 1379, 2164, 2239
BstH2I RGCGCY 1 cut(s) 882
BstHHI GCGC 1 cut(s) 881
BstKTI GATC 6 cut(s) 402, 847, 1072, 1383, 2058, 2130
BstMAI GTCTC 4 cut(s) 46, 233, 314, 2422
BstMBI GATC 6 cut(s) 399, 844, 1069, 1380, 2055, 2127
BstMWI GCNNNNNNNGC 8 cut(s) 815, 824, 956, 1015, 1139, 1241, 1952, 2336
BstNI CCWGG 3 cut(s) 104, 1202, 2292
BstNSI RCATGY 6 cut(s) 132, 246, 253, 1706, 2141, 2301
BstPAI GACNNNNGTC 1 cut(s) 1560
BstSCI CCNGG 3 cut(s) 102, 1200, 2290
BstSFI CTRYAG 5 cut(s) 1245, 1665, 1674, 1972, 2226
BstSLI GKGCMC 1 cut(s) 1279
BstV1I GCAGC 7 cut(s) 959, 1142, 1231, 1259, 1654, 1801, 1958
BstV2I GAAGAC 2 cut(s) 157, 1556
BstX2I RGATCY 1 cut(s) 2127
BstXI CCANNNNNNTGG 3 cut(s) 357, 1208, 1223
BstYI RGATCY 1 cut(s) 2127
Bsu15I ATCGAT 1 cut(s) 1896
BsuI GTATCC 1 cut(s) 204
BsuRI GGCC 4 cut(s) 111, 624, 2145, 2336
BsuTUI ATCGAT 1 cut(s) 1896
BtgI CCRYGG 2 cut(s) 1252, 2331
BtsCI GGATG 6 cut(s) 803, 1111, 1228, 1379, 2164, 2239
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 7 cut(s) 18, 182, 267, 635, 655, 756, 1980
Cac8I GCNNGC 3 cut(s) 1330, 1704, 2122
CfoI GCGC 1 cut(s) 881
Cfr10I RCCGGY 1 cut(s) 713
Cfr13I GGNCC 2 cut(s) 148, 623
ClaI ATCGAT 1 cut(s) 1896
Csp6I GTAC 4 cut(s) 180, 725, 895, 2295
CviQI GTAC 4 cut(s) 180, 725, 895, 2295
DdeI CTNAG 9 cut(s) 43, 259, 866, 1352, 1470, 1681, 1859, 1998, 2131
DinI GGCGCC 1 cut(s) 880
DpnI GATC 6 cut(s) 401, 846, 1071, 1382, 2057, 2129
DpnII GATC 6 cut(s) 399, 844, 1069, 1380, 2055, 2127
EaeI YGGCCR 3 cut(s) 109, 2143, 2334
Eam1104I CTCTTC 1 cut(s) 1386
EarI CTCTTC 1 cut(s) 1386
Eco130I CCWWGG 6 cut(s) 151, 223, 453, 1252, 1558, 2331
Eco31I GGTCTC 1 cut(s) 46
Eco47I GGWCC 1 cut(s) 148
Eco57I CTGAAG 4 cut(s) 528, 593, 1004, 2030
EcoNI CCTNNNNNAGG 1 cut(s) 546
EcoRI GAATTC 1 cut(s) 2379
EcoRII CCWGG 3 cut(s) 102, 1200, 2290
EcoT14I CCWWGG 6 cut(s) 151, 223, 453, 1252, 1558, 2331
EcoT22I ATGCAT 2 cut(s) 803, 1522
EgeI GGCGCC 1 cut(s) 880
EheI GGCGCC 1 cut(s) 880
ErhI CCWWGG 6 cut(s) 151, 223, 453, 1252, 1558, 2331
FalI AAGNNNNNCTT 2 cut(s) 323, 355
FbaI TGATCA 1 cut(s) 399
FblI GTMKAC 1 cut(s) 2302
FokI GGATG 6 cut(s) 810, 1098, 1235, 1386, 2171, 2246
FspBI CTAG 8 cut(s) 117, 875, 944, 1344, 1890, 1916, 2189, 2346
GlaI GCGC 1 cut(s) 880
GsuI CTGGAG 2 cut(s) 789, 1169
HaeII RGCGCY 1 cut(s) 882
HaeIII GGCC 4 cut(s) 111, 624, 2145, 2336
HapII CCGG 4 cut(s) 442, 714, 1371, 2053
HhaI GCGC 1 cut(s) 881
Hin1I GRCGYC 1 cut(s) 879
Hin6I GCGC 1 cut(s) 879
HinP1I GCGC 1 cut(s) 879
HincII GTYRAC 2 cut(s) 1043, 1104
HindII GTYRAC 2 cut(s) 1043, 1104
HindIII AAGCTT 3 cut(s) 316, 559, 1140
HinfI GANTC 6 cut(s) 470, 1601, 1893, 2112, 2180, 2203
HpaII CCGG 4 cut(s) 442, 714, 1371, 2053
HphI GGTGA 6 cut(s) 199, 370, 685, 1043, 1402, 1544
Hpy166II GTNNAC 9 cut(s) 182, 653, 895, 1043, 1104, 1410, 1462, 2220, 2303
Hpy188I TCNGA 7 cut(s) 487, 498, 547, 705, 1023, 1999, 2378
Hpy188III TCNNGA 8 cut(s) 358, 371, 410, 442, 917, 1067, 1916, 2346
Hpy8I GTNNAC 9 cut(s) 182, 653, 895, 1043, 1104, 1410, 1462, 2220, 2303
Hpy99I CGWCG 1 cut(s) 1318
HpyCH4III ACNGT 8 cut(s) 124, 630, 640, 659, 760, 899, 1436, 1466
HpyF10VI GCNNNNNNNGC 8 cut(s) 815, 824, 956, 1015, 1139, 1241, 1952, 2336
HpyF3I CTNAG 9 cut(s) 43, 259, 866, 1352, 1470, 1681, 1859, 1998, 2131
Hsp92I GRCGYC 1 cut(s) 879
HspAI GCGC 1 cut(s) 879
KasI GGCGCC 1 cut(s) 878
Kpn2I TCCGGA 1 cut(s) 441
Ksp22I TGATCA 1 cut(s) 399
Kzo9I GATC 6 cut(s) 399, 844, 1069, 1380, 2055, 2127
LmnI GCTCC 4 cut(s) 1150, 1193, 1937, 2359
Lsp1109I GCAGC 7 cut(s) 959, 1142, 1231, 1259, 1654, 1801, 1958
LweI GCATC 5 cut(s) 788, 1142, 1507, 1995, 2026
MaeI CTAG 8 cut(s) 117, 875, 944, 1344, 1890, 1916, 2189, 2346
MaeIII GTNAC 9 cut(s) 124, 425, 471, 917, 980, 1054, 1339, 2072, 2320
MalI GATC 6 cut(s) 401, 846, 1071, 1382, 2057, 2129
MboI GATC 6 cut(s) 399, 844, 1069, 1380, 2055, 2127
MboII GAAGA 8 cut(s) 157, 346, 679, 1403, 1556, 1723, 1844, 2334
MfeI CAATTG 1 cut(s) 2084
MflI RGATCY 1 cut(s) 2127
MhlI GDGCHC 2 cut(s) 1279, 2364
MlsI TGGCCA 1 cut(s) 111
MluCI AATT 9 cut(s) 228, 595, 786, 1179, 2012, 2084, 2150, 2379, 2416
MluNI TGGCCA 1 cut(s) 111
Mly113I GGCGCC 1 cut(s) 879
MlyI GAGTC 2 cut(s) 479, 2189
MmeI TCCRAC 2 cut(s) 666, 2387
Mox20I TGGCCA 1 cut(s) 111
Mph1103I ATGCAT 2 cut(s) 803, 1522
MroI TCCGGA 1 cut(s) 441
MroXI GAANNNNTTC 3 cut(s) 78, 341, 492
MscI TGGCCA 1 cut(s) 111
MseI TTAA 7 cut(s) 387, 929, 963, 1095, 1824, 2010, 2312
MslI CAYNNNNRTG 4 cut(s) 660, 1221, 1755, 2401
Msp20I TGGCCA 1 cut(s) 111
MspA1I CMGCKG 2 cut(s) 1325, 1814
MspCI CTTAAG 1 cut(s) 1094
MspI CCGG 4 cut(s) 442, 714, 1371, 2053
MspR9I CCNGG 3 cut(s) 104, 1202, 2292
MunI CAATTG 1 cut(s) 2084
MvaI CCWGG 3 cut(s) 104, 1202, 2292
MwoI GCNNNNNNNGC 8 cut(s) 815, 824, 956, 1015, 1139, 1241, 1952, 2336
NarI GGCGCC 1 cut(s) 879
NcoI CCATGG 2 cut(s) 1252, 2331
NdeII GATC 6 cut(s) 399, 844, 1069, 1380, 2055, 2127
NlaIV GGNNCC 2 cut(s) 880, 1939
NmuCI GTSAC 6 cut(s) 124, 471, 917, 980, 1054, 2072
NsiI ATGCAT 2 cut(s) 803, 1522
NspI RCATGY 6 cut(s) 132, 246, 253, 1706, 2141, 2301
PaeI GCATGC 1 cut(s) 1706
PciI ACATGT 4 cut(s) 128, 249, 2137, 2297
PdmI GAANNNNTTC 3 cut(s) 78, 341, 492
PfeI GAWTC 4 cut(s) 1601, 1893, 2112, 2203
PflFI GACNNNGTC 1 cut(s) 437
PleI GAGTC 2 cut(s) 478, 2188
PluTI GGCGCC 1 cut(s) 882
PpsI GAGTC 2 cut(s) 478, 2188
PscI ACATGT 4 cut(s) 128, 249, 2137, 2297
PshAI GACNNNNGTC 1 cut(s) 1560
PsiI TTATAA 1 cut(s) 557
Psp6I CCWGG 3 cut(s) 102, 1200, 2290
PspGI CCWGG 3 cut(s) 102, 1200, 2290
PspN4I GGNNCC 2 cut(s) 880, 1939
PspPI GGNCC 2 cut(s) 148, 623
PsrI GAACNNNNNNTAC 2 cut(s) 855, 887
PstI CTGCAG 2 cut(s) 1249, 1976
PsuI RGATCY 1 cut(s) 2127
PsyI GACNNNGTC 1 cut(s) 437
RsaI GTAC 4 cut(s) 181, 726, 896, 2296
RsaNI GTAC 4 cut(s) 180, 725, 895, 2295
RseI CAYNNNNRTG 4 cut(s) 660, 1221, 1755, 2401
SaqAI TTAA 7 cut(s) 387, 929, 963, 1095, 1824, 2010, 2312
Sau3AI GATC 6 cut(s) 399, 844, 1069, 1380, 2055, 2127
Sau96I GGNCC 2 cut(s) 148, 623
SchI GAGTC 2 cut(s) 479, 2189
ScrFI CCNGG 3 cut(s) 104, 1202, 2292
SduI GDGCHC 2 cut(s) 1279, 2364
SfaNI GCATC 5 cut(s) 788, 1142, 1507, 1995, 2026
SfcI CTRYAG 5 cut(s) 1245, 1665, 1674, 1972, 2226
SfoI GGCGCC 1 cut(s) 880
SinI GGWCC 1 cut(s) 148
SmiMI CAYNNNNRTG 4 cut(s) 660, 1221, 1755, 2401
SmlI CTYRAG 4 cut(s) 313, 410, 419, 1094
SmoI CTYRAG 4 cut(s) 313, 410, 419, 1094
SphI GCATGC 1 cut(s) 1706
Sse9I AATT 9 cut(s) 228, 595, 786, 1179, 2012, 2084, 2150, 2379, 2416
SsiI CCGC 6 cut(s) 621, 1325, 1812, 2080, 2231, 2337
SspDI GGCGCC 1 cut(s) 878
SspI AATATT 2 cut(s) 837, 1821
SspMI CTAG 8 cut(s) 117, 875, 944, 1344, 1890, 1916, 2189, 2346
StyD4I CCNGG 3 cut(s) 102, 1200, 2290
StyI CCWWGG 6 cut(s) 151, 223, 453, 1252, 1558, 2331
TaaI ACNGT 8 cut(s) 124, 630, 640, 659, 760, 899, 1436, 1466
TaqI TCGA 3 cut(s) 301, 1735, 1896
TasI AATT 9 cut(s) 228, 595, 786, 1179, 2012, 2084, 2150, 2379, 2416
TatI WGTACW 2 cut(s) 179, 894
TauI GCSGC 3 cut(s) 624, 1328, 2339
TfiI GAWTC 4 cut(s) 1601, 1893, 2112, 2203
Tru1I TTAA 7 cut(s) 387, 929, 963, 1095, 1824, 2010, 2312
Tru9I TTAA 7 cut(s) 387, 929, 963, 1095, 1824, 2010, 2312
TscAI CASTG 7 cut(s) 18, 189, 267, 635, 662, 763, 1987
TseFI GTSAC 6 cut(s) 124, 471, 917, 980, 1054, 2072
TseI GCWGC 7 cut(s) 947, 1130, 1244, 1247, 1642, 1814, 1971
Tsp45I GTSAC 6 cut(s) 124, 471, 917, 980, 1054, 2072
TspGWI ACGGA 3 cut(s) 1331, 1438, 1611
TspRI CASTG 7 cut(s) 18, 189, 267, 635, 662, 763, 1987
Tth111I GACNNNGTC 1 cut(s) 437
Vha464I CTTAAG 1 cut(s) 1094
VpaK11BI GGWCC 1 cut(s) 148
XagI CCTNNNNNAGG 1 cut(s) 546
XapI RAATTY 6 cut(s) 786, 1179, 2012, 2150, 2379, 2416
XbaI TCTAGA 2 cut(s) 1915, 2345
XceI RCATGY 6 cut(s) 132, 246, 253, 1706, 2141, 2301
XcmI CCANNNNNNNNNTGG 1 cut(s) 1259
XmiI GTMKAC 1 cut(s) 2302
XmnI GAANNNNTTC 3 cut(s) 78, 341, 492
XspI CTAG 8 cut(s) 117, 875, 944, 1344, 1890, 1916, 2189, 2346
Zsp2I ATGCAT 2 cut(s) 803, 1522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.