FvH4_4g11063
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0016797
Physical Location & Seq
Reverse (-)
14876776 .. 14879238
2463 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g11063.t1

Sequence Viewer

Length: 552 bp
ATGTGTGCATATACGTTGCCGACAAAGTACCGGTTCCCTAGAGGCGGCTCAAAATATAGGGGAGTGTCATTGCATAAGTGTGGGACATGGGAGACTCGGATGGGCCAATTTTTACGCAAAAAAATGAATGAAGGGGTTCTCATTTTGCATGCACGGATCAAGAGCCTTATCATTAACAGACCAGTTCCGGTATATTGGGTAAAAACCCTAACCTCTCTGCCTTTGCTGTTTTCTGTCGAGAGAGAGAGACCTGCCCACAGAGAGAGGGAGAGCGACGCCACCCAGCTCAGGGCTCCTCCGCCGCCGTCGGACCGGCGCTGGACGATCGGCGACCTTCTTGATCTGTATCTATGCCTGAAACAGAGCAATCACATAGTGGCACTGAAAGTCCTCTTCAAGAACCTGTTGCAACAATCTCAGGTTGAGCATCAGCTTCGCCCGGAAGTTGAAATACAAAGGCATCTTTGCCATCCCAATATTCTACGTCTGTATGGCTACTTTTATGATCAGAAAGGAGTTTATTTGATATTGGAATATGCTGCCAAAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

184

Amino Acids

21.44

Weight (kDa)

9.87

Isoelectric Point (pI)

53.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 116 - 183 4.5e-10 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 121 - 183 2e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 259
AciI CCGC 3 cut(s) 45, 299, 302
AclWI GGATC 1 cut(s) 164
AcyI GRCGYC 1 cut(s) 276
AdeI CACNNNGTG 1 cut(s) 376
AfaI GTAC 1 cut(s) 29
AfiI CCNNNNNNNGG 3 cut(s) 44, 288, 289
AgeI ACCGGT 1 cut(s) 30
AgsI TTSAA 2 cut(s) 397, 449
AluBI AGCT 2 cut(s) 286, 433
AluI AGCT 2 cut(s) 286, 433
Alw26I GTCTC 2 cut(s) 86, 241
AlwI GGATC 1 cut(s) 164
AoxI GGCC 1 cut(s) 103
ApeKI GCWGC 1 cut(s) 539
AsiGI ACCGGT 1 cut(s) 30
Asp700I GAANNNNTTC 1 cut(s) 135
AspLEI GCGC 1 cut(s) 318
AspS9I GGNCC 2 cut(s) 103, 310
AsuC2I CCSGG 1 cut(s) 440
AvaII GGWCC 1 cut(s) 310
BanII GRGCYC 1 cut(s) 295
BarI GAAGNNNNNNTAC 2 cut(s) 435, 467
BbvI GCAGC 1 cut(s) 526
BccI CCATC 2 cut(s) 94, 477
BceAI ACGGC 1 cut(s) 289
BcgI CGANNNNNNTGC 2 cut(s) 416, 450
BclI TGATCA 1 cut(s) 505
BcnI CCSGG 1 cut(s) 440
BcoDI GTCTC 2 cut(s) 86, 241
BfaI CTAG 1 cut(s) 39
BfoI RGCGCY 1 cut(s) 319
BfuAI ACCTGC 1 cut(s) 259
BisI GCNGC 3 cut(s) 46, 302, 540
BlsI GCNGC 3 cut(s) 47, 303, 541
Bme1390I CCNGG 1 cut(s) 440
Bme18I GGWCC 1 cut(s) 310
BmgT120I GGNCC 2 cut(s) 103, 310
BmiI GGNNCC 2 cut(s) 35, 294
BmrFI CCNGG 1 cut(s) 440
BmsI GCATC 2 cut(s) 436, 469
Bpu10I CCTNAGC 1 cut(s) 287
BpuMI CCSGG 1 cut(s) 440
BsaBI GATNNNNATC 1 cut(s) 345
BsaHI GRCGYC 1 cut(s) 276
BsaI GGTCTC 1 cut(s) 241
BsaWI WCCGGW 2 cut(s) 30, 187
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 288, 289
Bse118I RCCGGY 2 cut(s) 30, 312
Bse1I ACTGG 1 cut(s) 182
Bse3DI GCAATG 1 cut(s) 68
Bse8I GATNNNNATC 1 cut(s) 345
BseGI GGATG 2 cut(s) 105, 469
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 3 cut(s) 44, 288, 289
BseMI GCAATG 1 cut(s) 68
BseMII CTCAG 2 cut(s) 301, 431
BseNI ACTGG 1 cut(s) 182
BseRI GAGGAG 1 cut(s) 285
BseXI GCAGC 1 cut(s) 526
BseYI CCCAGC 1 cut(s) 282
Bsh1285I CGRYCG 1 cut(s) 327
BshFI GGCC 1 cut(s) 105
BshTI ACCGGT 1 cut(s) 30
BsiEI CGRYCG 1 cut(s) 327
BsiSI CCGG 4 cut(s) 31, 188, 313, 440
BslFI GGGAC 1 cut(s) 97
BslI CCNNNNNNNGG 3 cut(s) 44, 288, 289
BsmAI GTCTC 2 cut(s) 86, 241
BsmFI GGGAC 1 cut(s) 97
BsnI GGCC 1 cut(s) 105
Bso31I GGTCTC 1 cut(s) 241
Bsp1286I GDGCHC 1 cut(s) 295
Bsp143I GATC 4 cut(s) 156, 324, 340, 505
BspACI CCGC 3 cut(s) 45, 299, 302
BspANI GGCC 1 cut(s) 105
BspCNI CTCAG 2 cut(s) 300, 430
BspLI GGNNCC 2 cut(s) 35, 294
BspMI ACCTGC 1 cut(s) 259
BspPI GGATC 1 cut(s) 164
BspTNI GGTCTC 1 cut(s) 241
BsrDI GCAATG 1 cut(s) 68
BsrFI RCCGGY 2 cut(s) 30, 312
BsrI ACTGG 1 cut(s) 182
BssAI RCCGGY 2 cut(s) 30, 312
BssMI GATC 4 cut(s) 156, 324, 340, 505
BssNI GRCGYC 1 cut(s) 276
Bst6I CTCTTC 1 cut(s) 398
BstACI GRCGYC 1 cut(s) 276
BstC8I GCNNGC 1 cut(s) 150
BstDEI CTNAG 2 cut(s) 287, 417
BstF5I GGATG 2 cut(s) 105, 469
BstH2I RGCGCY 1 cut(s) 319
BstHHI GCGC 1 cut(s) 318
BstKTI GATC 4 cut(s) 159, 327, 343, 508
BstMAI GTCTC 2 cut(s) 86, 241
BstMBI GATC 4 cut(s) 156, 324, 340, 505
BstMCI CGRYCG 1 cut(s) 327
BstNSI RCATGY 1 cut(s) 152
BstSCI CCNGG 1 cut(s) 438
BstV1I GCAGC 1 cut(s) 526
BsuRI GGCC 1 cut(s) 105
BtsCI GGATG 2 cut(s) 105, 469
BtsIMutI CAGTG 1 cut(s) 380
BveI ACCTGC 1 cut(s) 259
Cac8I GCNNGC 1 cut(s) 150
CfoI GCGC 1 cut(s) 318
Cfr10I RCCGGY 2 cut(s) 30, 312
Cfr13I GGNCC 2 cut(s) 103, 310
CpoI CGGWCCG 1 cut(s) 310
CseI GACGC 1 cut(s) 284
Csp6I GTAC 1 cut(s) 28
CspAI ACCGGT 1 cut(s) 30
CspI CGGWCCG 1 cut(s) 310
CviAII CATG 2 cut(s) 87, 149
CviJI RGCY 7 cut(s) 48, 105, 165, 286, 293, 433, 495
CviKI_1 RGCY 7 cut(s) 48, 105, 165, 286, 293, 433, 495
CviQI GTAC 1 cut(s) 28
DdeI CTNAG 2 cut(s) 287, 417
DpnI GATC 4 cut(s) 158, 326, 342, 507
DpnII GATC 4 cut(s) 156, 324, 340, 505
DraIII CACNNNGTG 1 cut(s) 376
Eam1104I CTCTTC 1 cut(s) 398
EarI CTCTTC 1 cut(s) 398
EciI GGCGGA 1 cut(s) 288
Eco24I GRGCYC 1 cut(s) 295
Eco31I GGTCTC 1 cut(s) 241
Eco47I GGWCC 1 cut(s) 310
EcoT38I GRGCYC 1 cut(s) 295
FaeI CATG 2 cut(s) 90, 152
FaqI GGGAC 1 cut(s) 97
FatI CATG 2 cut(s) 86, 148
FbaI TGATCA 1 cut(s) 505
Fnu4HI GCNGC 3 cut(s) 46, 302, 540
FokI GGATG 2 cut(s) 112, 456
FriOI GRGCYC 1 cut(s) 295
Fsp4HI GCNGC 3 cut(s) 46, 302, 540
FspBI CTAG 1 cut(s) 39
GlaI GCGC 1 cut(s) 317
GluI GCNGC 3 cut(s) 46, 302, 540
GsaI CCCAGC 1 cut(s) 286
HaeII RGCGCY 1 cut(s) 319
HaeIII GGCC 1 cut(s) 105
HapII CCGG 4 cut(s) 31, 188, 313, 440
HgaI GACGC 1 cut(s) 284
HhaI GCGC 1 cut(s) 318
Hin1I GRCGYC 1 cut(s) 276
Hin1II CATG 2 cut(s) 90, 152
Hin6I GCGC 1 cut(s) 316
HinP1I GCGC 1 cut(s) 316
HinfI GANTC 1 cut(s) 94
HpaII CCGG 4 cut(s) 31, 188, 313, 440
Hpy188I TCNGA 3 cut(s) 99, 310, 510
Hpy188III TCNNGA 4 cut(s) 160, 238, 338, 397
Hpy99I CGWCG 2 cut(s) 278, 310
HpyAV CCTTC 2 cut(s) 125, 344
HpyCH4IV ACGT 2 cut(s) 14, 484
HpyCH4V TGCA 5 cut(s) 8, 73, 148, 152, 409
HpyF3I CTNAG 2 cut(s) 287, 417
HpySE526I ACGT 2 cut(s) 14, 484
Hsp92I GRCGYC 1 cut(s) 276
Hsp92II CATG 2 cut(s) 90, 152
HspAI GCGC 1 cut(s) 316
Ksp22I TGATCA 1 cut(s) 505
Kzo9I GATC 4 cut(s) 156, 324, 340, 505
LmnI GCTCC 1 cut(s) 298
Lsp1109I GCAGC 1 cut(s) 526
LweI GCATC 2 cut(s) 436, 469
MaeI CTAG 1 cut(s) 39
MaeII ACGT 2 cut(s) 14, 484
MalI GATC 4 cut(s) 158, 326, 342, 507
MboI GATC 4 cut(s) 156, 324, 340, 505
MboII GAAGA 1 cut(s) 385
MhlI GDGCHC 1 cut(s) 295
MluCI AATT 1 cut(s) 107
MlyI GAGTC 1 cut(s) 88
MmeI TCCRAC 1 cut(s) 288
MnlI CCTC 5 cut(s) 35, 223, 258, 306, 401
MroXI GAANNNNTTC 1 cut(s) 135
MseI TTAA 2 cut(s) 174, 550
MslI CAYNNNNRTG 1 cut(s) 78
MspI CCGG 4 cut(s) 31, 188, 313, 440
MspR9I CCNGG 1 cut(s) 440
NciI CCSGG 1 cut(s) 440
NdeII GATC 4 cut(s) 156, 324, 340, 505
NlaIII CATG 2 cut(s) 90, 152
NlaIV GGNNCC 2 cut(s) 35, 294
NspI RCATGY 1 cut(s) 152
PaeI GCATGC 1 cut(s) 152
PdmI GAANNNNTTC 1 cut(s) 135
PinAI ACCGGT 1 cut(s) 30
PkrI GCNGC 3 cut(s) 47, 303, 541
Ple19I CGATCG 1 cut(s) 327
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
PspFI CCCAGC 1 cut(s) 282
PspN4I GGNNCC 2 cut(s) 35, 294
PspPI GGNCC 2 cut(s) 103, 310
PvuI CGATCG 1 cut(s) 327
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
RseI CAYNNNNRTG 1 cut(s) 78
Rsr2I CGGWCCG 1 cut(s) 310
RsrII CGGWCCG 1 cut(s) 310
SaqAI TTAA 2 cut(s) 174, 550
SatI GCNGC 3 cut(s) 46, 302, 540
Sau3AI GATC 4 cut(s) 156, 324, 340, 505
Sau96I GGNCC 2 cut(s) 103, 310
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 440
SduI GDGCHC 1 cut(s) 295
SfaNI GCATC 2 cut(s) 436, 469
SinI GGWCC 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 78
SphI GCATGC 1 cut(s) 152
Sse9I AATT 1 cut(s) 107
SsiI CCGC 3 cut(s) 45, 299, 302
SspI AATATT 1 cut(s) 478
SspMI CTAG 1 cut(s) 39
StyD4I CCNGG 1 cut(s) 438
TaiI ACGT 2 cut(s) 17, 487
TaqI TCGA 1 cut(s) 237
TasI AATT 1 cut(s) 107
TauI GCSGC 2 cut(s) 48, 304
Tru1I TTAA 2 cut(s) 174, 550
Tru9I TTAA 2 cut(s) 174, 550
TscAI CASTG 1 cut(s) 387
TseI GCWGC 1 cut(s) 539
TspDTI ATGAA 2 cut(s) 140, 144
TspGWI ACGGA 1 cut(s) 169
TspRI CASTG 1 cut(s) 387
VpaK11BI GGWCC 1 cut(s) 310
XceI RCATGY 1 cut(s) 152
XmnI GAANNNNTTC 1 cut(s) 135
XspI CTAG 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.