FvH4_5g28220

Protein of unknown function (DUF707)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016797
Physical Location & Seq
Forward (+)
19406107 .. 19414001
7895 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g28220.t1

Sequence Viewer

Length: 795 bp
ATGGAGAAACACAAATCCAGCAAGCGCTCCCGAGACGAACGCGAGCGCCGTGACCGGGACAAGCATCGCAGCAGCCGCGACGCTTCTGATGACTATCACCGCCACCACCGCTCTCGATCCGACCGCGATTCTAAGCCCCACGACCACAGATCTCGCCGCGACAGGTCGTCGGAGCCGCGCGACCACTTTGAGAGGGAGAGGAGTGAGGGTGTGGATGATTTCAACGATATCCACTTTGAAGCCACCCATGCGACCAAGCCAGAATTTGGTTCTGTGTTTTTGATTTATTGGCAATTTGAGATATGGGTAGTTCTTGACTTAGATGAGACGCTAATATGTGCATACGAAACATCTAGTGTGCCTGCCATTGTTTGGACTCAAGCAACGGATGCTGGGATGAAGTGGTTGAAGCAACGGATGTGTATCTTCGTACTAGGTAGGCAGGCTGGAGGCAATCTCAAGCGGTTCAGTAGGTTGGAAGCTTTGAAAAACTCAACGCCTTCAAAGAGCTTGCTGGCCATTGCAGTTGGAATAAAACAGAAAGGAATAGTGGACAAAATTGTTCGGAAGTTTTTATCAAGTGACTTTGTGGTAATGCTTTTCCATTATGATGGTGCTGTGGATAGGTGGAGGGATTTACATTGGAGTGATCCTGCCATTCATGTGTCTGATGAACCAAACAAAATGGATGAGGGACTTGAAATGTCACAGCCAGCACTTGATCCTGTCAAATCGGAGATGTATCATCCAATCACTGCACGTGTAAAGAAATCTAAGGTGCACTGGTCAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF707

Protein Analysis

265

Amino Acids

30.94

Weight (kDa)

8.95

Isoelectric Point (pI)

50.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF707 PF05212 154 - 224 2.2e-23 Protein of unknown function (DUF707)
DUF707 PF05212 230 - 261 8.8e-06 Protein of unknown function (DUF707)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 266, 372
AccBSI CCGCTC 1 cut(s) 111
AccII CGCG 6 cut(s) 42, 78, 126, 159, 178, 180
AciI CCGC 7 cut(s) 76, 100, 109, 124, 157, 176, 463
AclWI GGATC 3 cut(s) 111, 644, 716
AcoI YGGCCR 1 cut(s) 516
AcsI RAATTY 1 cut(s) 263
AcvI CACGTG 1 cut(s) 761
AfaI GTAC 1 cut(s) 432
AfeI AGCGCT 1 cut(s) 26
AfiI CCNNNNNNNGG 3 cut(s) 55, 266, 372
AflIII ACRYGT 1 cut(s) 760
AgsI TTSAA 6 cut(s) 223, 239, 409, 487, 504, 701
AhdI GACNNNNNGTC 1 cut(s) 166
AluBI AGCT 2 cut(s) 482, 510
AluI AGCT 2 cut(s) 482, 510
Alw21I GWGCWC 1 cut(s) 783
Alw26I GTCTC 2 cut(s) 27, 320
Alw44I GTGCAC 1 cut(s) 779
AlwI GGATC 3 cut(s) 111, 644, 716
Ama87I CYCGRG 1 cut(s) 30
Aor51HI AGCGCT 1 cut(s) 26
AoxI GGCC 1 cut(s) 516
ApaLI GTGCAC 1 cut(s) 779
ApeKI GCWGC 2 cut(s) 69, 72
ApoI RAATTY 1 cut(s) 263
AspLEI GCGC 3 cut(s) 27, 48, 180
AsuC2I CCSGG 1 cut(s) 56
AsuHPI GGTGA 1 cut(s) 89
AvaI CYCGRG 1 cut(s) 30
BaeGI GKGCMC 1 cut(s) 783
BalI TGGCCA 1 cut(s) 518
BbrPI CACGTG 1 cut(s) 761
Bbv12I GWGCWC 1 cut(s) 783
BbvI GCAGC 2 cut(s) 81, 84
BccI CCATC 1 cut(s) 605
BceAI ACGGC 1 cut(s) 33
BcnI CCSGG 1 cut(s) 56
BcoDI GTCTC 2 cut(s) 27, 320
BfaI CTAG 2 cut(s) 354, 434
BfoI RGCGCY 2 cut(s) 28, 49
BglII AGATCT 1 cut(s) 149
BisI GCNGC 5 cut(s) 70, 73, 76, 157, 176
BlsI GCNGC 5 cut(s) 71, 74, 77, 158, 177
Bme1390I CCNGG 1 cut(s) 56
BmeRI GACNNNNNGTC 1 cut(s) 166
BmeT110I CYCGRG 1 cut(s) 30
BmiI GGNNCC 1 cut(s) 174
BmrFI CCNGG 1 cut(s) 56
BmsI GCATC 2 cut(s) 73, 379
BplI GAGNNNNNCTC 2 cut(s) 441, 473
BpmI CTGGAG 1 cut(s) 468
BpuEI CTTGAG 2 cut(s) 363, 443
BpuMI CCSGG 1 cut(s) 56
BsaAI YACGTR 1 cut(s) 761
BsaBI GATNNNNATC 2 cut(s) 93, 422
BsaXI ACNNNNNCTCC 2 cut(s) 193, 223
Bsc4I CCNNNNNNNGG 3 cut(s) 55, 266, 372
Bse1I ACTGG 1 cut(s) 788
Bse3DI GCAATG 1 cut(s) 519
Bse8I GATNNNNATC 2 cut(s) 93, 422
BseGI GGATG 6 cut(s) 220, 394, 402, 423, 694, 745
BseJI GATNNNNATC 2 cut(s) 93, 422
BseLI CCNNNNNNNGG 3 cut(s) 55, 266, 372
BseMI GCAATG 1 cut(s) 519
BseNI ACTGG 1 cut(s) 788
BseRI GAGGAG 1 cut(s) 214
BseSI GKGCMC 1 cut(s) 783
BseXI GCAGC 2 cut(s) 81, 84
BseYI CCCAGC 1 cut(s) 392
BsgI GTGCAG 1 cut(s) 741
Bsh1236I CGCG 6 cut(s) 42, 78, 126, 159, 178, 180
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 1 cut(s) 518
BsiEI CGRYCG 1 cut(s) 124
BsiHKAI GWGCWC 1 cut(s) 783
BsiHKCI CYCGRG 1 cut(s) 30
BsiSI CCGG 1 cut(s) 55
BslFI GGGAC 2 cut(s) 71, 708
BslI CCNNNNNNNGG 3 cut(s) 55, 266, 372
BsmAI GTCTC 2 cut(s) 27, 320
BsmBI CGTCTC 2 cut(s) 27, 320
BsmFI GGGAC 2 cut(s) 71, 708
BsnI GGCC 1 cut(s) 518
BsoBI CYCGRG 1 cut(s) 30
Bsp1286I GDGCHC 1 cut(s) 783
Bsp143I GATC 4 cut(s) 116, 149, 649, 721
BspACI CCGC 7 cut(s) 76, 100, 109, 124, 157, 176, 463
BspANI GGCC 1 cut(s) 518
BspFNI CGCG 6 cut(s) 42, 78, 126, 159, 178, 180
BspLI GGNNCC 1 cut(s) 174
BspPI GGATC 3 cut(s) 111, 644, 716
BsrBI CCGCTC 1 cut(s) 111
BsrDI GCAATG 1 cut(s) 519
BsrI ACTGG 1 cut(s) 788
BssMI GATC 4 cut(s) 116, 149, 649, 721
BstAPI GCANNNNNTGC 1 cut(s) 389
BstBAI YACGTR 1 cut(s) 761
BstC8I GCNNGC 7 cut(s) 23, 44, 363, 444, 512, 516, 714
BstDEI CTNAG 3 cut(s) 132, 319, 774
BstF5I GGATG 6 cut(s) 220, 394, 402, 423, 694, 745
BstFNI CGCG 6 cut(s) 42, 78, 126, 159, 178, 180
BstH2I RGCGCY 2 cut(s) 28, 49
BstHHI GCGC 3 cut(s) 27, 48, 180
BstKTI GATC 4 cut(s) 119, 152, 652, 724
BstMAI GTCTC 2 cut(s) 27, 320
BstMBI GATC 4 cut(s) 116, 149, 649, 721
BstMCI CGRYCG 1 cut(s) 124
BstMWI GCNNNNNNNGC 4 cut(s) 75, 108, 248, 389
BstSCI CCNGG 1 cut(s) 54
BstSLI GKGCMC 1 cut(s) 783
BstUI CGCG 6 cut(s) 42, 78, 126, 159, 178, 180
BstV1I GCAGC 2 cut(s) 81, 84
BstX2I RGATCY 1 cut(s) 149
BstXI CCANNNNNNTGG 1 cut(s) 611
BstYI RGATCY 1 cut(s) 149
BsuRI GGCC 1 cut(s) 518
BtgZI GCGATG 1 cut(s) 50
BtsCI GGATG 6 cut(s) 220, 394, 402, 423, 694, 745
BtsI GCAGTG 1 cut(s) 753
BtsIMutI CAGTG 3 cut(s) 753, 781, 795
Cac8I GCNNGC 7 cut(s) 23, 44, 363, 444, 512, 516, 714
CfoI GCGC 3 cut(s) 27, 48, 180
CseI GACGC 2 cut(s) 89, 337
Csp6I GTAC 1 cut(s) 431
CviAII CATG 2 cut(s) 248, 662
CviQI GTAC 1 cut(s) 431
DdeI CTNAG 3 cut(s) 132, 319, 774
DpnI GATC 4 cut(s) 118, 151, 651, 723
DpnII GATC 4 cut(s) 116, 149, 649, 721
DriI GACNNNNNGTC 1 cut(s) 166
EaeI YGGCCR 1 cut(s) 516
Eam1105I GACNNNNNGTC 1 cut(s) 166
Eco32I GATATC 1 cut(s) 229
Eco47III AGCGCT 1 cut(s) 26
Eco72I CACGTG 1 cut(s) 761
Eco88I CYCGRG 1 cut(s) 30
EcoRV GATATC 1 cut(s) 229
Esp3I CGTCTC 2 cut(s) 27, 320
FaeI CATG 2 cut(s) 251, 665
FaiI YATR 6 cut(s) 249, 304, 337, 343, 609, 663
FaqI GGGAC 2 cut(s) 71, 708
FatI CATG 2 cut(s) 247, 661
Fnu4HI GCNGC 5 cut(s) 70, 73, 76, 157, 176
FokI GGATG 6 cut(s) 227, 401, 409, 430, 701, 732
Fsp4HI GCNGC 5 cut(s) 70, 73, 76, 157, 176
FspBI CTAG 2 cut(s) 354, 434
GlaI GCGC 3 cut(s) 26, 47, 179
GluI GCNGC 5 cut(s) 70, 73, 76, 157, 176
GsaI CCCAGC 1 cut(s) 396
GsuI CTGGAG 1 cut(s) 468
HaeII RGCGCY 2 cut(s) 28, 49
HaeIII GGCC 1 cut(s) 518
HapII CCGG 1 cut(s) 55
HgaI GACGC 2 cut(s) 89, 337
HhaI GCGC 3 cut(s) 27, 48, 180
Hin1II CATG 2 cut(s) 251, 665
Hin6I GCGC 3 cut(s) 25, 46, 178
HinP1I GCGC 3 cut(s) 25, 46, 178
HindIII AAGCTT 1 cut(s) 480
HinfI GANTC 2 cut(s) 128, 376
HpaII CCGG 1 cut(s) 55
HphI GGTGA 1 cut(s) 89
Hpy166II GTNNAC 2 cut(s) 553, 781
Hpy188I TCNGA 6 cut(s) 88, 121, 172, 567, 670, 736
Hpy188III TCNNGA 3 cut(s) 30, 114, 314
Hpy8I GTNNAC 2 cut(s) 553, 781
Hpy99I CGWCG 2 cut(s) 83, 172
HpyAV CCTTC 1 cut(s) 510
HpyCH4IV ACGT 1 cut(s) 760
HpyCH4V TGCA 4 cut(s) 341, 524, 758, 781
HpyF10VI GCNNNNNNNGC 4 cut(s) 75, 108, 248, 389
HpyF3I CTNAG 3 cut(s) 132, 319, 774
HpySE526I ACGT 1 cut(s) 760
Hsp92II CATG 2 cut(s) 251, 665
HspAI GCGC 3 cut(s) 25, 46, 178
Kzo9I GATC 4 cut(s) 116, 149, 649, 721
LmnI GCTCC 2 cut(s) 32, 172
Lsp1109I GCAGC 2 cut(s) 81, 84
LweI GCATC 2 cut(s) 73, 379
MaeI CTAG 2 cut(s) 354, 434
MaeII ACGT 1 cut(s) 760
MaeIII GTNAC 3 cut(s) 50, 581, 705
MalI GATC 4 cut(s) 118, 151, 651, 723
MbiI CCGCTC 1 cut(s) 111
MboI GATC 4 cut(s) 116, 149, 649, 721
MboII GAAGA 1 cut(s) 418
MflI RGATCY 1 cut(s) 149
MhlI GDGCHC 1 cut(s) 783
MlsI TGGCCA 1 cut(s) 518
MluCI AATT 3 cut(s) 263, 293, 558
MluNI TGGCCA 1 cut(s) 518
MlyI GAGTC 1 cut(s) 370
MmeI TCCRAC 4 cut(s) 144, 150, 456, 508
MnlI CCTC 6 cut(s) 186, 192, 199, 443, 624, 685
Mox20I TGGCCA 1 cut(s) 518
MscI TGGCCA 1 cut(s) 518
MslI CAYNNNNRTG 3 cut(s) 609, 645, 662
Msp20I TGGCCA 1 cut(s) 518
MspI CCGG 1 cut(s) 55
MspR9I CCNGG 1 cut(s) 56
MvnI CGCG 6 cut(s) 42, 78, 126, 159, 178, 180
MwoI GCNNNNNNNGC 4 cut(s) 75, 108, 248, 389
NciI CCSGG 1 cut(s) 56
NdeII GATC 4 cut(s) 116, 149, 649, 721
NlaIII CATG 2 cut(s) 251, 665
NlaIV GGNNCC 1 cut(s) 174
NmuCI GTSAC 3 cut(s) 50, 581, 705
PcsI WCGNNNNNNNCGW 1 cut(s) 46
PfeI GAWTC 1 cut(s) 128
PflMI CCANNNNNTGG 2 cut(s) 266, 372
PkrI GCNGC 5 cut(s) 71, 74, 77, 158, 177
PleI GAGTC 1 cut(s) 370
PmaCI CACGTG 1 cut(s) 761
PmlI CACGTG 1 cut(s) 761
PpsI GAGTC 1 cut(s) 370
Ppu21I YACGTR 1 cut(s) 761
PspCI CACGTG 1 cut(s) 761
PspFI CCCAGC 1 cut(s) 392
PspN4I GGNNCC 1 cut(s) 174
PsuI RGATCY 1 cut(s) 149
RsaI GTAC 1 cut(s) 432
RsaNI GTAC 1 cut(s) 431
RseI CAYNNNNRTG 3 cut(s) 609, 645, 662
SatI GCNGC 5 cut(s) 70, 73, 76, 157, 176
Sau3AI GATC 4 cut(s) 116, 149, 649, 721
SchI GAGTC 1 cut(s) 370
ScrFI CCNGG 1 cut(s) 56
SduI GDGCHC 1 cut(s) 783
SetI ASST 8 cut(s) 167, 439, 476, 484, 512, 629, 763, 780
SfaNI GCATC 2 cut(s) 73, 379
SmiMI CAYNNNNRTG 3 cut(s) 609, 645, 662
SmlI CTYRAG 2 cut(s) 378, 458
SmoI CTYRAG 2 cut(s) 378, 458
Sse9I AATT 3 cut(s) 263, 293, 558
SsiI CCGC 7 cut(s) 76, 100, 109, 124, 157, 176, 463
SspMI CTAG 2 cut(s) 354, 434
StyD4I CCNGG 1 cut(s) 54
TaiI ACGT 1 cut(s) 763
TaqI TCGA 1 cut(s) 115
TasI AATT 3 cut(s) 263, 293, 558
TauI GCSGC 3 cut(s) 78, 159, 178
TfiI GAWTC 1 cut(s) 128
TscAI CASTG 3 cut(s) 760, 788, 795
TseFI GTSAC 3 cut(s) 50, 581, 705
TseI GCWGC 2 cut(s) 69, 72
Tsp45I GTSAC 3 cut(s) 50, 581, 705
TspDTI ATGAA 3 cut(s) 413, 650, 687
TspGWI ACGGA 2 cut(s) 401, 430
TspRI CASTG 3 cut(s) 760, 788, 795
Van91I CCANNNNNTGG 2 cut(s) 266, 372
VneI GTGCAC 1 cut(s) 779
XapI RAATTY 1 cut(s) 263
XspI CTAG 2 cut(s) 354, 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.