FvH4_5g28160

vesicle-mediated transport

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016797
Physical Location & Seq
Forward (+)
19350143 .. 19358691
8549 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g28160.t1

Sequence Viewer

Length: 690 bp
ATGGTGATGAATGCCGGTATTAGCTCCAAAGGTTGGTATTTTGGATCAAGGAAAACAAAGGAGTATGTTCTGAGTCAAAAGCCGATGAGATATCTTCGGGTGAAAATCTATGGAGGATTTGTTAACATGTGCCTGGTCGATTGGTTAATGTCAATTTCTATGCCAATTCTTTCTGAGGCTGGAAGAAGTGGAGTGGAGAGGCAGAATCCTATCATCAGCAAGAATAGGGAAGTATCAGCTATTGAAACTATGGTGAAGAGGACTAGAGAGTTGGGGTTCGGGTCAAAAAGACCTAAAGCAGAAAAAATAAACAGACTTAAAATTAGAGAAAGAGCTCATCCTAGCTCTAGAAACACCAACAATGTCATCCAGAAAGGTTTGTGCGGCATGGTTGGGAGGATGGTTAAAAGCAATGAAACCAAGTCACTGATTTTCGATTTACCAAAAGCAATCACAATTGAGGAGATAATTGGAGCTGGCTGGAAACTAGCTATGGTGATGAATGCAAGTATTTGCTCCAGAGGTTTCAATCCTTTGTGTTGGATCAAGGAAACTAAAAGAGCTTTAGTGATGCAAGGTAAACATGCAGAAGCAATGGTGGAACTGTCAAAGATATGCCTTGTTTGGCGGATTTTCCCTCCCGAGGAGTCTTCGAAAAGTGCTGCAATGCCAATTCCAAACCACATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169
Pfam Domains
Protein Families
No domains found.

Protein Analysis

230

Amino Acids

25.86

Weight (kDa)

10.11

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 33
AciI CCGC 2 cut(s) 384, 628
AclWI GGATC 2 cut(s) 52, 551
AfiI CCNNNNNNNGG 2 cut(s) 33, 643
AflIII ACRYGT 2 cut(s) 126, 684
AgsI TTSAA 2 cut(s) 245, 529
AjnI CCWGG 1 cut(s) 132
AluBI AGCT 7 cut(s) 24, 239, 335, 345, 476, 491, 563
AluI AGCT 7 cut(s) 24, 239, 335, 345, 476, 491, 563
Alw21I GWGCWC 1 cut(s) 337
AlwI GGATC 2 cut(s) 52, 551
Ama87I CYCGRG 1 cut(s) 641
ApeKI GCWGC 1 cut(s) 662
AsuHPI GGTGA 4 cut(s) 16, 112, 265, 508
AsuII TTCGAA 1 cut(s) 653
AvaI CYCGRG 1 cut(s) 641
BanII GRGCYC 1 cut(s) 337
BbsI GAAGAC 1 cut(s) 642
Bbv12I GWGCWC 1 cut(s) 337
BbvI GCAGC 1 cut(s) 649
BccI CCATC 1 cut(s) 394
BciT130I CCWGG 1 cut(s) 134
BfaI CTAG 4 cut(s) 264, 342, 348, 488
BisI GCNGC 2 cut(s) 385, 663
BlsI GCNGC 2 cut(s) 386, 664
Bme1390I CCNGG 1 cut(s) 134
BmeT110I CYCGRG 1 cut(s) 641
BmrFI CCNGG 1 cut(s) 134
BmsI GCATC 1 cut(s) 561
BpiI GAAGAC 1 cut(s) 642
BpmI CTGGAG 1 cut(s) 502
Bpu14I TTCGAA 1 cut(s) 653
BsaJI CCNNGG 1 cut(s) 642
BsaXI ACNNNNNCTCC 2 cut(s) 105, 135
Bsc4I CCNNNNNNNGG 2 cut(s) 33, 643
Bse118I RCCGGY 1 cut(s) 14
Bse3DI GCAATG 3 cut(s) 418, 600, 672
BseBI CCWGG 1 cut(s) 134
BseDI CCNNGG 1 cut(s) 642
BseGI GGATG 3 cut(s) 337, 366, 405
BseLI CCNNNNNNNGG 2 cut(s) 33, 643
BseMI GCAATG 3 cut(s) 418, 600, 672
BseMII CTCAG 2 cut(s) 62, 165
BseRI GAGGAG 2 cut(s) 476, 659
BseXI GCAGC 1 cut(s) 649
BsiHKAI GWGCWC 1 cut(s) 337
BsiHKCI CYCGRG 1 cut(s) 641
BsiSI CCGG 1 cut(s) 15
BslI CCNNNNNNNGG 2 cut(s) 33, 643
BsmI GAATGC 2 cut(s) 16, 508
BsoBI CYCGRG 1 cut(s) 641
Bsp119I TTCGAA 1 cut(s) 653
Bsp1286I GDGCHC 1 cut(s) 337
Bsp143I GATC 2 cut(s) 44, 543
BspACI CCGC 2 cut(s) 384, 628
BspCNI CTCAG 2 cut(s) 63, 166
BspPI GGATC 2 cut(s) 52, 551
BspT104I TTCGAA 1 cut(s) 653
BsrDI GCAATG 3 cut(s) 418, 600, 672
BsrFI RCCGGY 1 cut(s) 14
BssAI RCCGGY 1 cut(s) 14
BssECI CCNNGG 1 cut(s) 642
BssMI GATC 2 cut(s) 44, 543
Bst2UI CCWGG 1 cut(s) 134
Bst4CI ACNGT 1 cut(s) 606
Bst6I CTCTTC 1 cut(s) 251
BstBI TTCGAA 1 cut(s) 653
BstC8I GCNNGC 1 cut(s) 478
BstDEI CTNAG 2 cut(s) 71, 174
BstF5I GGATG 3 cut(s) 337, 366, 405
BstKTI GATC 2 cut(s) 47, 546
BstMBI GATC 2 cut(s) 44, 543
BstNI CCWGG 1 cut(s) 134
BstNSI RCATGY 3 cut(s) 130, 587, 688
BstSCI CCNGG 1 cut(s) 132
BstV1I GCAGC 1 cut(s) 649
BstV2I GAAGAC 1 cut(s) 642
BtsCI GGATG 3 cut(s) 337, 366, 405
BtsIMutI CAGTG 1 cut(s) 425
Cac8I GCNNGC 1 cut(s) 478
Cfr10I RCCGGY 1 cut(s) 14
CviAII CATG 4 cut(s) 127, 388, 584, 685
DdeI CTNAG 2 cut(s) 71, 174
DpnI GATC 2 cut(s) 46, 545
DpnII GATC 2 cut(s) 44, 543
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
EciI GGCGGA 1 cut(s) 643
Ecl136II GAGCTC 1 cut(s) 335
Eco24I GRGCYC 1 cut(s) 337
Eco32I GATATC 1 cut(s) 92
Eco53kI GAGCTC 1 cut(s) 335
Eco88I CYCGRG 1 cut(s) 641
EcoICRI GAGCTC 1 cut(s) 335
EcoRII CCWGG 1 cut(s) 132
EcoRV GATATC 1 cut(s) 92
EcoT38I GRGCYC 1 cut(s) 337
FaeI CATG 4 cut(s) 130, 391, 587, 688
FatI CATG 4 cut(s) 126, 387, 583, 684
Fnu4HI GCNGC 2 cut(s) 385, 663
FokI GGATG 3 cut(s) 324, 353, 412
FriOI GRGCYC 1 cut(s) 337
Fsp4HI GCNGC 2 cut(s) 385, 663
FspBI CTAG 4 cut(s) 264, 342, 348, 488
GluI GCNGC 2 cut(s) 385, 663
GsuI CTGGAG 1 cut(s) 502
HapII CCGG 1 cut(s) 15
Hin1II CATG 4 cut(s) 130, 391, 587, 688
HincII GTYRAC 1 cut(s) 124
HindII GTYRAC 1 cut(s) 124
HinfI GANTC 3 cut(s) 73, 205, 647
HpaI GTTAAC 1 cut(s) 124
HpaII CCGG 1 cut(s) 15
HphI GGTGA 4 cut(s) 16, 112, 265, 508
Hpy166II GTNNAC 2 cut(s) 124, 581
Hpy188I TCNGA 2 cut(s) 72, 175
Hpy188III TCNNGA 4 cut(s) 348, 370, 519, 641
Hpy8I GTNNAC 2 cut(s) 124, 581
HpyCH4III ACNGT 1 cut(s) 606
HpyCH4V TGCA 4 cut(s) 506, 574, 587, 665
HpyF3I CTNAG 2 cut(s) 71, 174
Hsp92II CATG 4 cut(s) 130, 391, 587, 688
KspAI GTTAAC 1 cut(s) 124
Kzo9I GATC 2 cut(s) 44, 543
LmnI GCTCC 3 cut(s) 29, 473, 521
LpnPI CCDG 8 cut(s) 28, 119, 146, 165, 383, 462, 466, 532
Lsp1109I GCAGC 1 cut(s) 649
LweI GCATC 1 cut(s) 561
MaeI CTAG 4 cut(s) 264, 342, 348, 488
MaeIII GTNAC 1 cut(s) 423
MalI GATC 2 cut(s) 46, 545
MboI GATC 2 cut(s) 44, 543
MboII GAAGA 4 cut(s) 86, 195, 268, 642
MfeI CAATTG 1 cut(s) 456
MhlI GDGCHC 1 cut(s) 337
MluCI AATT 6 cut(s) 153, 165, 321, 456, 468, 672
MlyI GAGTC 2 cut(s) 82, 656
MmeI TCCRAC 1 cut(s) 521
MnlI CCTC 9 cut(s) 107, 169, 192, 252, 390, 454, 515, 637, 648
MseI TTAA 4 cut(s) 123, 146, 318, 405
MspI CCGG 1 cut(s) 15
MspR9I CCNGG 1 cut(s) 134
MunI CAATTG 1 cut(s) 456
Mva1269I GAATGC 2 cut(s) 16, 508
MvaI CCWGG 1 cut(s) 134
NdeII GATC 2 cut(s) 44, 543
NlaIII CATG 4 cut(s) 130, 391, 587, 688
NmuCI GTSAC 1 cut(s) 423
NspI RCATGY 3 cut(s) 130, 587, 688
NspV TTCGAA 1 cut(s) 653
PciI ACATGT 2 cut(s) 126, 684
PctI GAATGC 2 cut(s) 16, 508
PfeI GAWTC 1 cut(s) 205
PflMI CCANNNNNTGG 1 cut(s) 33
PkrI GCNGC 2 cut(s) 386, 664
PleI GAGTC 2 cut(s) 81, 655
PpsI GAGTC 2 cut(s) 81, 655
PscI ACATGT 2 cut(s) 126, 684
Psp124BI GAGCTC 1 cut(s) 337
Psp6I CCWGG 1 cut(s) 132
PspGI CCWGG 1 cut(s) 132
SacI GAGCTC 1 cut(s) 337
SaqAI TTAA 4 cut(s) 123, 146, 318, 405
SatI GCNGC 2 cut(s) 385, 663
Sau3AI GATC 2 cut(s) 44, 543
SchI GAGTC 2 cut(s) 82, 656
ScrFI CCNGG 1 cut(s) 134
SduI GDGCHC 1 cut(s) 337
SfaNI GCATC 1 cut(s) 561
SfuI TTCGAA 1 cut(s) 653
Sse9I AATT 6 cut(s) 153, 165, 321, 456, 468, 672
SsiI CCGC 2 cut(s) 384, 628
SspMI CTAG 4 cut(s) 264, 342, 348, 488
SstI GAGCTC 1 cut(s) 337
StyD4I CCNGG 1 cut(s) 132
TaaI ACNGT 1 cut(s) 606
TaqI TCGA 3 cut(s) 138, 435, 653
TasI AATT 6 cut(s) 153, 165, 321, 456, 468, 672
TauI GCSGC 1 cut(s) 387
TfiI GAWTC 1 cut(s) 205
Tru1I TTAA 4 cut(s) 123, 146, 318, 405
Tru9I TTAA 4 cut(s) 123, 146, 318, 405
TscAI CASTG 1 cut(s) 432
TseFI GTSAC 1 cut(s) 423
TseI GCWGC 1 cut(s) 662
Tsp45I GTSAC 1 cut(s) 423
TspDTI ATGAA 3 cut(s) 23, 429, 515
TspRI CASTG 1 cut(s) 432
Van91I CCANNNNNTGG 1 cut(s) 33
XbaI TCTAGA 1 cut(s) 347
XceI RCATGY 3 cut(s) 130, 587, 688
XspI CTAG 4 cut(s) 264, 342, 348, 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.