FvH4_4g12980

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011107
Physical Location & Seq
Reverse (-)
16643847 .. 16649022
5176 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g12980.t1

Sequence Viewer

Length: 2514 bp
ATGGAAGCAAATCGAAATCGAACTTCCAGGCCACCTTTGGGCACCTGCACCCGCAGCCAGTCCCAGATCCAGCTCCACCCCACCCGCTCGGGTCGGGTCATCAACAGCCTCCTCACCGCCTCCGGTACAGACCGGAAAAGACCCAGACCATCCGATTCTTCCGACGGCGACGTACTTCTCAGCTCTCTGATTAAGGATCTCCGTGTCAAACGAGTCTTCTCCCAAACCTACAAGCCGCCCGAAAATCTAGTATCCGGCACCGATTTCTGTTTGCCTGAACCGGAGGATAATTCAAAGGAGTCGAAGACCGAGAGTAAGGAGCCTGAAAATTTGGGATTAGGTTCCGATCTCGGCGGTGCATCCGGTTCCCATCCGGATTCTACGCTGACGTCATCACCTGAGGAGAAGGCGAATGGAGTTGAGCTCAAAATCGATAGCGAAACCAAAACGGTTCTTAAGACTCGAAAGAGAGTGTTTAAAGCTCCCGGCTCGTTTAGTTATAGAAGAATGCTTCCTTATCTGAAGGATATCACCCAGGACAACTCTTGCATGCTAGAGTTTTGTGAACTGCCGAATCGTGGTGAAGGTTTGGAAGGAAACAAGCTTGTTTCTGATAACCAAGAGACTTATCTACACCAATTTACCACGCAGAATTTCTCTAAAGAGTGTCATACCTTTGATTCTGATACTCAGCTGGAGACTGTACGAGTTCCTTCCCATGTTCCATCTAATGGTGATCACTTCAGCTCAGAATCTCCTGAATATGTTTCGAAATCACCACCTACAGTAGTTTCTGATCAGTGTCCGCCAAATCCTCCACTTGATGGACATAATGAGAATATTGATTTGGGATTATCTTGTGAAGTTCAGAAGTTAAATGTCTCGGAACGTTTTTCCACTTCAGCTATGGAGGATTCTCAAGTTAACACGGATGCTGTAATGGTCATGTCAACTGATGGTAATCCACCAGTGGAGAAAATGGGGACTGCTGTTAAGGATGATATTTTCTCCTCTTCCGTAGATCAAACTGTGAAAGATATAAATCCCCAGTCTTCTTCAAGCATGGATGAGAGCTATTCATGTAAGGATAATGAAGTGGTTCAACACAAGGAAAACTCAAAACAGGCTCAGTATGAAGGGATTGAAAATATAGATATAGGGTATCCTTGTGATGTCCGAAGTCAAAATCATACAAATGAGCAGAGAGTTGGTAATACTGAAGAGGTTTTGAATCAGATATGTGACTTGCATGAAGAATCTATCCAGATGACACCGCCAGATGCTGAGATACTTGATAAACTACAGGTGGAAGTAAAAGTAATCGGTAGAGCTGAATATGTTCATCCGAGTACAGATCAAGGTCTTGGGAAGCCAAGCAGTGGAAATAACTATAGAGATGTTGCACAGGTTGATCTTAGGCATGAATGTAGTCCAAAGAGAAAACTAGTTTTAAATCCTTGTTCAAGGCTGAAATTATTTAAGAGTCCGGGTTCAGTCAGCTACAGAAGATTACTTCCGTTTCTTATGGATATGGACAAAGGTGCTTCAGTATCCAGTCACTCTGAAAGTACCCTTAAGGATCTGGTACAAAGGCAACCCTTGACTCCCTCTCAGCTAGGACCTCCAATGGAGATATCAAATGTTCTTTTGTTTCATGCAGAGCCTGGCACACCAACACCTCAATTGACAAATGCTAATGGTTCATCTAATATTGATGGGCCAAATATAATATCTCCTGAACATGTCACTGGATCTCAGATTCCATCTAATGTACAAAAGGAAGTGCATGGTCAACATTCTGCGTCAGATAAAAAGAGTAAATTGGACACTTCCCCTAATGTTATTTGGAATTTGGGACAGGCAGCAGAACTATCAGTCCCTATATTGGGTCCTAAGAAGGCCAAATCTTCTTCTGAAAAACCTGGTGTGAGAACATCCGGTCCAGAACTACCAGTCACAAGTGTGTTATCTCTTGGTACTGTTGACGACTGCATAAGCTCAAAAACAGCCTTAAATGGTGGTGGGAAGCCAGTTGAAGCCAATAGCTTAGGTCAAAACTCATCTGAAATTGATGCCACAGTTTCTTCAATTACTCAGGCAGTTGCAGTCAGAAAAGGGATCCTTAAAAGAAATCCACGAGGATGCAGAGGACTCTGTACTTGTTTGAATTGTGCTTCGTTTCGACTCCATGCAGAGAGAGCTTTTGAGTTCTCGAGAAATCAAATGCAAGATGCTGAAGAAGTAGCTTTGCATTTGATGAAGGAATTATCAAAATTGCGTAATTTATTAGAAAAGTCAGCTGCGACAAATGATCATGTTGTCACTTTTGATGAGGTGAAAAGCGCTTGCAGGGAAGCATCTGAAGCAGAAGACCTTGCAAGAAACCGTCTTGGGGAAATGAATTACGATCTCAACATCCACTGCAGAATAACATGCTTGCACGAACCAAGGGTCAAGTTTGCAAATCACAGTGAGGAAAAAGTCATTCCAAAAGCCAAGTGTTCAAGCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

838

Amino Acids

91.76

Weight (kDa)

5.68

Isoelectric Point (pI)

48.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011107)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23740 AT4G14120
fragaria_vesca FvH4_4g12980 FvH4_4g12980 FvH4_4g12980 FvH4_4g12980
malus_domestica MD04G1019900.v1.1
prunus_persica Prupe.1G169400_v2.0.a1
pyrus_communis pycom04g01590
rosa_chinensis RchiOBHm_Chr4g0412661
rosa_laevigata RLG00000008269
rosa_multiflora Rmu_co8037358.1_g000001 Rmu_sc0001238.1_g000004
rosa_roxburghii Rroxscaffold_5G00356860
rosa_rugosa Rorug04G0115700
rosa_samantha Rh4AG174900 Rh4BG174300 Rh4CG185800 Rh4DG170800
rosa_wichuraiana Rw4G014660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 53
AatII GACGTC 1 cut(s) 392
Acc36I ACCTGC 1 cut(s) 53
AccB1I GGYRCC 2 cut(s) 41, 257
AccB7I CCANNNNNTGG 3 cut(s) 731, 824, 1379
AccBSI CCGCTC 1 cut(s) 87
AccIII TCCGGA 1 cut(s) 373
AciI CCGC 7 cut(s) 52, 85, 117, 236, 354, 806, 1274
AclI AACGTT 1 cut(s) 889
AclWI GGATC 6 cut(s) 61, 204, 1587, 1759, 2113, 2126
AcsI RAATTY 3 cut(s) 328, 652, 1849
AcuI CTGAAG 7 cut(s) 542, 727, 885, 1239, 1530, 2256, 2382
AcyI GRCGYC 1 cut(s) 389
AfaI GTAC 9 cut(s) 127, 174, 705, 1351, 1570, 1587, 1773, 1978, 2158
AfeI AGCGCT 1 cut(s) 2344
AfiI CCNNNNNNNGG 8 cut(s) 38, 578, 731, 824, 1379, 1885, 1886, 2392
AflII CTTAAG 2 cut(s) 455, 1574
AflIII ACRYGT 1 cut(s) 1741
AhlI ACTAGT 1 cut(s) 1444
AjnI CCWGG 4 cut(s) 26, 534, 1663, 1921
AjuI GAANNNNNNNTTGG 2 cut(s) 830, 862
AleI CACNNNNGTG 1 cut(s) 1961
AloI GAACNNNNNNTCC 4 cut(s) 1860, 1892, 1924, 1956
Alw21I GWGCWC 1 cut(s) 426
Alw26I GTCTC 3 cut(s) 617, 692, 886
AlwI GGATC 6 cut(s) 61, 204, 1587, 1759, 2113, 2126
AlwNI CAGNNNCTG 2 cut(s) 1283, 1664
Ama87I CYCGRG 2 cut(s) 88, 2212
Aor13HI TCCGGA 1 cut(s) 373
Aor51HI AGCGCT 1 cut(s) 2344
AoxI GGCC 3 cut(s) 29, 1718, 1899
ApeKI GCWGC 3 cut(s) 54, 1862, 2300
ApoI RAATTY 3 cut(s) 328, 652, 1849
ArsI GACNNNNNNTTYG 2 cut(s) 2436, 2468
Asp700I GAANNNNTTC 2 cut(s) 1098, 1338
AspLEI GCGC 1 cut(s) 2345
AspS9I GGNCC 4 cut(s) 1619, 1718, 1889, 1940
AsuC2I CCSGG 2 cut(s) 486, 1488
AsuHPI GGTGA 7 cut(s) 106, 387, 523, 593, 746, 768, 2347
AsuII TTCGAA 1 cut(s) 770
AvaI CYCGRG 2 cut(s) 88, 2212
AvaII GGWCC 3 cut(s) 1619, 1889, 1940
AxyI CCTNAGG 1 cut(s) 399
BaeGI GKGCMC 1 cut(s) 44
BamHI GGATCC 1 cut(s) 2118
BanI GGYRCC 2 cut(s) 41, 257
BanII GRGCYC 1 cut(s) 426
BauI CACGAG 1 cut(s) 2136
BbsI GAAGAC 4 cut(s) 208, 311, 1044, 2376
Bbv12I GWGCWC 1 cut(s) 426
BbvI GCAGC 3 cut(s) 66, 1874, 2287
BccI CCATC 7 cut(s) 157, 378, 733, 818, 950, 1709, 1771
BceAI ACGGC 1 cut(s) 181
BciT130I CCWGG 4 cut(s) 28, 536, 1665, 1923
BciVI GTATCC 3 cut(s) 262, 1173, 1561
BclI TGATCA 3 cut(s) 736, 796, 2311
BcnI CCSGG 2 cut(s) 486, 1488
BcoDI GTCTC 3 cut(s) 617, 692, 886
BcuI ACTAGT 1 cut(s) 1444
BfaI CTAG 4 cut(s) 248, 554, 1445, 1616
BfmI CTRYAG 5 cut(s) 783, 1301, 1390, 1501, 2422
BfoI RGCGCY 1 cut(s) 2346
BfrI CTTAAG 2 cut(s) 455, 1574
BfuAI ACCTGC 1 cut(s) 53
BfuI GTATCC 3 cut(s) 262, 1173, 1561
BisI GCNGC 4 cut(s) 55, 236, 1863, 2301
BlsI GCNGC 4 cut(s) 56, 237, 1864, 2302
Bme1390I CCNGG 6 cut(s) 28, 486, 536, 1488, 1665, 1923
Bme18I GGWCC 3 cut(s) 1619, 1889, 1940
BmeT110I CYCGRG 2 cut(s) 88, 2212
BmgT120I GGNCC 4 cut(s) 1619, 1718, 1889, 1940
BmiI GGNNCC 7 cut(s) 43, 259, 321, 343, 367, 1890, 2120
BmrFI CCNGG 6 cut(s) 28, 486, 536, 1488, 1665, 1923
BmrI ACTGGG 1 cut(s) 1042
BmsI GCATC 7 cut(s) 368, 922, 1270, 2062, 2132, 2221, 2366
BmuI ACTGGG 1 cut(s) 1042
BpiI GAAGAC 4 cut(s) 208, 311, 1044, 2376
BplI GAGNNNNNCTC 2 cut(s) 408, 440
BpmI CTGGAG 1 cut(s) 716
Bpu10I CCTNAGC 1 cut(s) 2047
Bpu14I TTCGAA 1 cut(s) 770
BpuEI CTTGAG 1 cut(s) 903
BpuMI CCSGG 2 cut(s) 486, 1488
Bsa29I ATCGAT 1 cut(s) 432
BsaBI GATNNNNATC 2 cut(s) 960, 1041
BsaHI GRCGYC 1 cut(s) 389
BsaJI CCNNGG 2 cut(s) 534, 2447
BsaWI WCCGGW 6 cut(s) 122, 132, 280, 362, 373, 1937
Bsc4I CCNNNNNNNGG 8 cut(s) 38, 578, 731, 824, 1379, 1885, 1886, 2392
Bse1I ACTGG 7 cut(s) 58, 968, 1048, 1554, 1753, 1952, 2030
Bse21I CCTNAGG 1 cut(s) 399
Bse8I GATNNNNATC 2 cut(s) 960, 1041
BseAI TCCGGA 1 cut(s) 373
BseBI CCWGG 4 cut(s) 28, 536, 1665, 1923
BseCI ATCGAT 1 cut(s) 432
BseDI CCNNGG 2 cut(s) 534, 2447
BseJI GATNNNNATC 2 cut(s) 960, 1041
BseLI CCNNNNNNNGG 8 cut(s) 38, 578, 731, 824, 1379, 1885, 1886, 2392
BseMII CTCAG 9 cut(s) 193, 390, 704, 762, 1142, 1275, 1625, 1769, 2108
BseNI ACTGG 7 cut(s) 58, 968, 1048, 1554, 1753, 1952, 2030
BseRI GAGGAG 3 cut(s) 101, 416, 1000
BseSI GKGCMC 1 cut(s) 44
BseXI GCAGC 3 cut(s) 66, 1874, 2287
BsgI GTGCAG 1 cut(s) 31
BshFI GGCC 3 cut(s) 31, 1720, 1901
BshNI GGYRCC 2 cut(s) 41, 257
BshVI ATCGAT 1 cut(s) 432
BsiHKAI GWGCWC 1 cut(s) 426
BsiHKCI CYCGRG 2 cut(s) 88, 2212
BsiSI CCGG 9 cut(s) 123, 133, 255, 281, 363, 374, 486, 1487, 1938
BslFI GGGAC 4 cut(s) 46, 997, 1862, 1869
BslI CCNNNNNNNGG 8 cut(s) 38, 578, 731, 824, 1379, 1885, 1886, 2392
BsmAI GTCTC 3 cut(s) 617, 692, 886
BsmFI GGGAC 4 cut(s) 46, 997, 1862, 1869
BsmI GAATGC 1 cut(s) 513
BsnI GGCC 3 cut(s) 31, 1720, 1901
BsoBI CYCGRG 2 cut(s) 88, 2212
Bsp119I TTCGAA 1 cut(s) 770
Bsp1286I GDGCHC 2 cut(s) 44, 426
Bsp13I TCCGGA 1 cut(s) 373
Bsp1407I TGTACA 1 cut(s) 1771
BspACI CCGC 7 cut(s) 52, 85, 117, 236, 354, 806, 1274
BspANI GGCC 3 cut(s) 31, 1720, 1901
BspCNI CTCAG 9 cut(s) 192, 391, 703, 761, 1141, 1276, 1624, 1768, 2107
BspDI ATCGAT 1 cut(s) 432
BspEI TCCGGA 1 cut(s) 373
BspLI GGNNCC 7 cut(s) 43, 259, 321, 343, 367, 1890, 2120
BspMAI CTGCAG 1 cut(s) 2426
BspMI ACCTGC 1 cut(s) 53
BspPI GGATC 6 cut(s) 61, 204, 1587, 1759, 2113, 2126
BspT104I TTCGAA 1 cut(s) 770
BspT107I GGYRCC 2 cut(s) 41, 257
BspTI CTTAAG 2 cut(s) 455, 1574
BsrBI CCGCTC 1 cut(s) 87
BsrGI TGTACA 1 cut(s) 1771
BsrI ACTGG 7 cut(s) 58, 968, 1048, 1554, 1753, 1952, 2030
BssECI CCNNGG 2 cut(s) 534, 2447
BssNI GRCGYC 1 cut(s) 389
BssSI CACGAG 1 cut(s) 2136
BssT1I CCWWGG 1 cut(s) 2447
Bst2BI CACGAG 1 cut(s) 2136
Bst2UI CCWGG 4 cut(s) 28, 536, 1665, 1923
Bst4CI ACNGT 8 cut(s) 451, 703, 787, 1030, 1981, 2080, 2387, 2471
Bst6I CTCTTC 2 cut(s) 1018, 1215
BstACI GRCGYC 1 cut(s) 389
BstAFI CTTAAG 2 cut(s) 455, 1574
BstAUI TGTACA 1 cut(s) 1771
BstBI TTCGAA 1 cut(s) 770
BstC8I GCNNGC 3 cut(s) 551, 2347, 2438
BstH2I RGCGCY 1 cut(s) 2346
BstHHI GCGC 1 cut(s) 2345
BstMAI GTCTC 3 cut(s) 617, 692, 886
BstMWI GCNNNNNNNGC 3 cut(s) 54, 2198, 2363
BstNI CCWGG 4 cut(s) 28, 536, 1665, 1923
BstNSI RCATGY 3 cut(s) 553, 1745, 2436
BstSCI CCNGG 6 cut(s) 26, 484, 534, 1486, 1663, 1921
BstSFI CTRYAG 5 cut(s) 783, 1301, 1390, 1501, 2422
BstSLI GKGCMC 1 cut(s) 44
BstV1I GCAGC 3 cut(s) 66, 1874, 2287
BstV2I GAAGAC 4 cut(s) 208, 311, 1044, 2376
BstX2I RGATCY 5 cut(s) 66, 196, 1579, 1751, 2118
BstYI RGATCY 5 cut(s) 66, 196, 1579, 1751, 2118
Bsu15I ATCGAT 1 cut(s) 432
Bsu36I CCTNAGG 1 cut(s) 399
BsuI GTATCC 3 cut(s) 262, 1173, 1561
BsuRI GGCC 3 cut(s) 31, 1720, 1901
BsuTUI ATCGAT 1 cut(s) 432
BtsI GCAGTG 2 cut(s) 1384, 2419
BtsIMutI CAGTG 6 cut(s) 806, 975, 1384, 1746, 2419, 2476
BveI ACCTGC 1 cut(s) 53
Cac8I GCNNGC 3 cut(s) 551, 2347, 2438
CaiI CAGNNNCTG 2 cut(s) 1283, 1664
CfoI GCGC 1 cut(s) 2345
Cfr13I GGNCC 4 cut(s) 1619, 1718, 1889, 1940
ClaI ATCGAT 1 cut(s) 432
CseI GACGC 1 cut(s) 1791
CsiI ACCWGGT 1 cut(s) 1921
Csp6I GTAC 9 cut(s) 126, 173, 704, 1350, 1569, 1586, 1772, 1977, 2157
CviQI GTAC 9 cut(s) 126, 173, 704, 1350, 1569, 1586, 1772, 1977, 2157
DraI TTTAAA 2 cut(s) 478, 1452
Eam1104I CTCTTC 2 cut(s) 1018, 1215
EarI CTCTTC 2 cut(s) 1018, 1215
EciI GGCGGA 1 cut(s) 795
Ecl136II GAGCTC 1 cut(s) 424
Eco130I CCWWGG 1 cut(s) 2447
Eco24I GRGCYC 1 cut(s) 426
Eco32I GATATC 2 cut(s) 529, 1635
Eco47I GGWCC 3 cut(s) 1619, 1889, 1940
Eco47III AGCGCT 1 cut(s) 2344
Eco53kI GAGCTC 1 cut(s) 424
Eco57I CTGAAG 7 cut(s) 542, 727, 885, 1239, 1530, 2256, 2382
Eco81I CCTNAGG 1 cut(s) 399
Eco88I CYCGRG 2 cut(s) 88, 2212
EcoICRI GAGCTC 1 cut(s) 424
EcoO109I RGGNCCY 2 cut(s) 1619, 1889
EcoRII CCWGG 4 cut(s) 26, 534, 1663, 1921
EcoRV GATATC 2 cut(s) 529, 1635
EcoT14I CCWWGG 1 cut(s) 2447
EcoT38I GRGCYC 1 cut(s) 426
ErhI CCWWGG 1 cut(s) 2447
FalI AAGNNNNNCTT 2 cut(s) 2106, 2138
FaqI GGGAC 4 cut(s) 46, 997, 1862, 1869
FauI CCCGC 2 cut(s) 59, 92
FbaI TGATCA 3 cut(s) 736, 796, 2311
Fnu4HI GCNGC 4 cut(s) 55, 236, 1863, 2301
FriOI GRGCYC 1 cut(s) 426
Fsp4HI GCNGC 4 cut(s) 55, 236, 1863, 2301
FspBI CTAG 4 cut(s) 248, 554, 1445, 1616
GlaI GCGC 1 cut(s) 2344
GluI GCNGC 4 cut(s) 55, 236, 1863, 2301
GsuI CTGGAG 1 cut(s) 716
HaeII RGCGCY 1 cut(s) 2346
HaeIII GGCC 3 cut(s) 31, 1720, 1901
HapII CCGG 9 cut(s) 123, 133, 255, 281, 363, 374, 486, 1487, 1938
HgaI GACGC 1 cut(s) 1791
HhaI GCGC 1 cut(s) 2345
Hin1I GRCGYC 1 cut(s) 389
Hin6I GCGC 1 cut(s) 2343
HinP1I GCGC 1 cut(s) 2343
HincII GTYRAC 4 cut(s) 925, 951, 1793, 1984
HindII GTYRAC 4 cut(s) 925, 951, 1793, 1984
HindIII AAGCTT 1 cut(s) 602
HpaI GTTAAC 1 cut(s) 925
HpaII CCGG 9 cut(s) 123, 133, 255, 281, 363, 374, 486, 1487, 1938
HphI GGTGA 7 cut(s) 106, 387, 523, 593, 746, 768, 2347
Hpy166II GTNNAC 5 cut(s) 566, 925, 951, 1793, 1984
Hpy188III TCNNGA 7 cut(s) 374, 758, 1264, 1736, 1943, 2212, 2214
Hpy8I GTNNAC 5 cut(s) 566, 925, 951, 1793, 1984
Hpy99I CGWCG 2 cut(s) 167, 173
HpyAV CCTTC 8 cut(s) 400, 517, 578, 587, 723, 1130, 1891, 2254
HpyCH4III ACNGT 8 cut(s) 451, 703, 787, 1030, 1981, 2080, 2387, 2471
HpyCH4IV ACGT 3 cut(s) 171, 389, 889
HpyF10VI GCNNNNNNNGC 3 cut(s) 54, 2198, 2363
HpySE526I ACGT 3 cut(s) 171, 389, 889
Hsp92I GRCGYC 1 cut(s) 389
HspAI GCGC 1 cut(s) 2343
Kpn2I TCCGGA 1 cut(s) 373
Ksp22I TGATCA 3 cut(s) 736, 796, 2311
KspAI GTTAAC 1 cut(s) 925
LmnI GCTCC 3 cut(s) 78, 319, 487
Lsp1109I GCAGC 3 cut(s) 66, 1874, 2287
LweI GCATC 7 cut(s) 368, 922, 1270, 2062, 2132, 2221, 2366
MabI ACCWGGT 1 cut(s) 1921
MaeI CTAG 4 cut(s) 248, 554, 1445, 1616
MaeII ACGT 3 cut(s) 171, 389, 889
MaeIII GTNAC 5 cut(s) 1241, 1556, 1744, 1954, 2320
MbiI CCGCTC 1 cut(s) 87
MfeI CAATTG 1 cut(s) 1682
MflI RGATCY 5 cut(s) 66, 196, 1579, 1751, 2118
MhlI GDGCHC 2 cut(s) 44, 426
MlyI GAGTC 7 cut(s) 222, 308, 454, 1492, 1597, 2145, 2178
MmeI TCCRAC 1 cut(s) 186
MroI TCCGGA 1 cut(s) 373
MroXI GAANNNNTTC 2 cut(s) 1098, 1338
MslI CAYNNNNRTG 3 cut(s) 1194, 1961, 2140
MspA1I CMGCKG 2 cut(s) 694, 2300
MspCI CTTAAG 2 cut(s) 455, 1574
MspI CCGG 9 cut(s) 123, 133, 255, 281, 363, 374, 486, 1487, 1938
MspR9I CCNGG 6 cut(s) 28, 486, 536, 1488, 1665, 1923
MunI CAATTG 1 cut(s) 1682
Mva1269I GAATGC 1 cut(s) 513
MvaI CCWGG 4 cut(s) 28, 536, 1665, 1923
MwoI GCNNNNNNNGC 3 cut(s) 54, 2198, 2363
NciI CCSGG 2 cut(s) 486, 1488
NlaIV GGNNCC 7 cut(s) 43, 259, 321, 343, 367, 1890, 2120
NmeAIII GCCGAG 1 cut(s) 330
NmuCI GTSAC 5 cut(s) 1241, 1556, 1744, 1954, 2320
NspI RCATGY 3 cut(s) 553, 1745, 2436
NspV TTCGAA 1 cut(s) 770
OliI CACNNNNGTG 1 cut(s) 1961
PaeI GCATGC 1 cut(s) 553
PaeR7I CTCGAG 1 cut(s) 2212
PaqCI CACCTGC 1 cut(s) 53
PciI ACATGT 1 cut(s) 1741
PctI GAATGC 1 cut(s) 513
PdmI GAANNNNTTC 2 cut(s) 1098, 1338
PfeI GAWTC 9 cut(s) 155, 377, 574, 680, 752, 914, 1231, 1256, 1759
PflMI CCANNNNNTGG 3 cut(s) 731, 824, 1379
PkrI GCNGC 4 cut(s) 56, 237, 1864, 2302
PleI GAGTC 7 cut(s) 221, 307, 454, 1491, 1597, 2145, 2178
PpsI GAGTC 7 cut(s) 221, 307, 454, 1491, 1597, 2145, 2178
PpuMI RGGWCCY 2 cut(s) 1619, 1889
PscI ACATGT 1 cut(s) 1741
Psp124BI GAGCTC 1 cut(s) 426
Psp1406I AACGTT 1 cut(s) 889
Psp5II RGGWCCY 2 cut(s) 1619, 1889
Psp6I CCWGG 4 cut(s) 26, 534, 1663, 1921
PspGI CCWGG 4 cut(s) 26, 534, 1663, 1921
PspN4I GGNNCC 7 cut(s) 43, 259, 321, 343, 367, 1890, 2120
PspPI GGNCC 4 cut(s) 1619, 1718, 1889, 1940
PspPPI RGGWCCY 2 cut(s) 1619, 1889
PstI CTGCAG 1 cut(s) 2426
PstNI CAGNNNCTG 2 cut(s) 1283, 1664
PsuI RGATCY 5 cut(s) 66, 196, 1579, 1751, 2118
PvuII CAGCTG 2 cut(s) 694, 2300
RsaI GTAC 9 cut(s) 127, 174, 705, 1351, 1570, 1587, 1773, 1978, 2158
RsaNI GTAC 9 cut(s) 126, 173, 704, 1350, 1569, 1586, 1772, 1977, 2157
RseI CAYNNNNRTG 3 cut(s) 1194, 1961, 2140
SacI GAGCTC 1 cut(s) 426
SatI GCNGC 4 cut(s) 55, 236, 1863, 2301
Sau96I GGNCC 4 cut(s) 1619, 1718, 1889, 1940
SchI GAGTC 7 cut(s) 222, 308, 454, 1492, 1597, 2145, 2178
ScrFI CCNGG 6 cut(s) 28, 486, 536, 1488, 1665, 1923
SduI GDGCHC 2 cut(s) 44, 426
SexAI ACCWGGT 1 cut(s) 1921
SfaNI GCATC 7 cut(s) 368, 922, 1270, 2062, 2132, 2221, 2366
SfcI CTRYAG 5 cut(s) 783, 1301, 1390, 1501, 2422
Sfr274I CTCGAG 1 cut(s) 2212
SfuI TTCGAA 1 cut(s) 770
SinI GGWCC 3 cut(s) 1619, 1889, 1940
SlaI CTCGAG 1 cut(s) 2212
SmiMI CAYNNNNRTG 3 cut(s) 1194, 1961, 2140
SmlI CTYRAG 4 cut(s) 455, 918, 1574, 2212
SmoI CTYRAG 4 cut(s) 455, 918, 1574, 2212
SpeI ACTAGT 1 cut(s) 1444
SphI GCATGC 1 cut(s) 553
SsiI CCGC 7 cut(s) 52, 85, 117, 236, 354, 806, 1274
SspI AATATT 2 cut(s) 841, 1711
SspMI CTAG 4 cut(s) 248, 554, 1445, 1616
SstI GAGCTC 1 cut(s) 426
StyD4I CCNGG 6 cut(s) 26, 484, 534, 1486, 1663, 1921
StyI CCWWGG 1 cut(s) 2447
TaaI ACNGT 8 cut(s) 451, 703, 787, 1030, 1981, 2080, 2387, 2471
TaiI ACGT 3 cut(s) 174, 392, 892
TaqI TCGA 8 cut(s) 13, 19, 302, 432, 463, 770, 2182, 2213
TaqII GACCGA 1 cut(s) 323
TatI WGTACW 3 cut(s) 1349, 1771, 2156
TauI GCSGC 1 cut(s) 238
TfiI GAWTC 9 cut(s) 155, 377, 574, 680, 752, 914, 1231, 1256, 1759
TscAI CASTG 6 cut(s) 806, 975, 1384, 1753, 2426, 2476
TseFI GTSAC 5 cut(s) 1241, 1556, 1744, 1954, 2320
TseI GCWGC 3 cut(s) 54, 1862, 2300
Tsp45I GTSAC 5 cut(s) 1241, 1556, 1744, 1954, 2320
TspGWI ACGGA 4 cut(s) 191, 944, 1006, 1506
TspRI CASTG 6 cut(s) 806, 975, 1384, 1753, 2426, 2476
Van91I CCANNNNNTGG 3 cut(s) 731, 824, 1379
Vha464I CTTAAG 2 cut(s) 455, 1574
VpaK11BI GGWCC 3 cut(s) 1619, 1889, 1940
XapI RAATTY 3 cut(s) 328, 652, 1849
XceI RCATGY 3 cut(s) 553, 1745, 2436
XcmI CCANNNNNNNNNTGG 2 cut(s) 34, 904
XhoI CTCGAG 1 cut(s) 2212
XmnI GAANNNNTTC 2 cut(s) 1098, 1338
XspI CTAG 4 cut(s) 248, 554, 1445, 1616
ZraI GACGTC 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.