Rroxscaffold_5G00356860

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0011107
Physical Location & Seq
Reverse (-)
36319223 .. 36324051
4829 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00356860.1

Sequence Viewer

Length: 2622 bp
ATGAAAGCAAATCGAACTCCCAAGCGACCTCCGGGCATCCGCACCCGCAGCCAATCCGAGATCCAGCTCCACCGCACCCGCTCGGGTCGGGTCATCAACAGCTACCTCACTGGCTCCGAGTCGGACCGGAAAAGACCCAGACCAATGGATTCTTCCGACGGCAACGTCCTCCTCAACTCCCTGATTAAGGACCTCCGAGTCAAACGGGTCTTTTCCCAAACCTACAAGCCGCCCGAGAATCTAATCAACGACGCCAAATCCGGCGCCGTATTCTCTCCGGCTGAACCGGACGGTGATTCAAAGGAGGCGAAGAGTGATCGGGTCGAGAGTTGTGGAAATCGGTTTGATTGCTCAGCTCCAGATATGGATGACTCCAAGAGTAAAGAGCCTGAAAATTTGGGATTAGGGTTTCTGGCTGAATCCCCAAATTCTGCAGATAAGATTGGCGGTGGTTCCGGTTCCGATCCGGAATCTTCTCAGACCTCACCACCTGAGGCGAAGGAAAACGGAGTTGAGCTCAAAATCGATAATGTAACCAAAATGGTCCTTAAGACTCGAAAGAGAGTGTTTAAAGCGCCCAGCTCGTTTAGTTATAGAAGAATGCTTCCTTATCTGAAGGATATCACCAAAGACAACTCGTGCATGCTAGATTTCTGTGAATTGCCAAATCGTGGTAAAGGTTTGGAAGGAAAATCTTTCCGGAGTAAGCTTGTTTCTGATAATCAAGAAACTTCTGTACATCAATTTACTACACAAAATTTCTCTACAGAGTGTCATGACATCGATTGTTCTACTCAGCTGGAAACTGTACAAGTTTCTTCCCATGCTTTATCTAGTGGTAATGACTTCAGTTCAGAATCTCCTGAATATGCTCCTAAATCACTGCCTATAGTAGTGGTTGATCAGTGTCTGCCAAAGCCACCTGCTGATGGACAGAATGAGAAAATTGATTTGGGAGTATCTTGTGAAGTTCAGAAGTTAAATTTCTCAGAACCTTTTCGCTCTTCAGCTATGGAGGAGTCTCAAATTAACACGGATGGTGTAATGGTCATGTGTACTGATGATAATACACCAGTGGATAATGTGGATACTGCTGTTAAGGATGATATTGTGTCCTCTTCCGAAGATCAAAACATGAAAGACATAAATCCCCAGTCATCTTCAATCATGGATGACAGATATTCATCTAAGGACAATGAAGTGGTTCAGCACAAGGAAAACTCAAACCAGGCTCAGAATGAAGGGATTAGAAAAACTGATATGGGGTATCCTTGTAAAGTCCGACATCCAAATCATATAAATGAGCAGAGAGTTGGTGATGCTGAAGAGGCTTTGAATCAGATATGTGATTTGAATGAAGAATCTATCCAGATGACACCGCCAGATGCTGAGATTCTTAATAAATTACAGGTGGAAGTAAAAGGAATTGGTAGAGCCGGATATCTTCATCAGAGTACAGACCAAGGTCTTGGGAAGACAAGCAGTGGTATTAATCATATAGATGCTTTGTGGGTTGATCTTGGACATGAATGTAGTCCTAAGAGAAAACCGGTTTTAAATCCATGTTCAAGGCTGAAGTTATTTAAAAGTCCGGGGTCAGTCAGCTATAGAAGATTGCTTCCATTTCTTATGAATATGGAAAAAAGGACTTCAGTATCCAGTCATTTTGAAAGTACTGTTAAGGATCTGGAACAAAGGCAACCATCGACTCCCCCTCAGCTAGGACCTCCGATGGATAAATCAAATGTTGTTTTGTTTCATACAGAGCCTAGCACAACAACACCTCAATTGACAAATGCTAATGATTCATCTAATGTTGATGGACCAAATTTAACATCTCCTAAATGTGTCAGTGGATCTCAAATGTCATCTAATGTACAAAAGGAAATGCATGGTCAACATTCTGTGTCAGGTAAACAGAGTAAACTGGACATTTCCCCTGACATAGTCGTGAGTTTGGGACAGGAAGCTGAACTACCTGCCCCTATATTGGGTCCTAATAAGACTGAATCTCCTTCCGAAAAAGATGGTGCGAGAATATCCTATCCAGAATTACCAGTCACAAGGGTGTTATCTCTTGGCACTGTCAAGGACTGCATACGCTCAAAAGCAACCTTAACTGATGGTGGGAAGCCAGTGGAAGCCAATAACTTGGGTCATAACTCATCTGAAATTGATGCCACAGTTTCTTCAGTTACTCAGGCGGATGCAGTCAGAAAAGGGATCCTTAAAAGAAATCCACGAGGATGCAGAGGACTCTGCACTTGTTTGAATTGTGCCTCGTTTCGACTCCATGCAGAGAGAGCATTTGAGTTCTCACAAAATCAGATGCAAGATGCTGAAGAAGTAGCTTTGGATTTGATGGCGGAATTATCAAAATTGCGTAAATTATTAGAAAGGTCAGCTGCCACTGCAAATGATGATGTCGTCACTTTAAATGAGGTGAAAAGTGCTTGCAGGAAAGCATCTGAAGCAGAAGTCCTTGCAAGAAACCGTCTGGGGGAAATGAATTATGATCTCAACATTCACTGCAGAATCACATGCTTGCATGAACCAAGGGTCAGGTTTGCAAATCACAGTGAAGAAAAAGTCATTCCAAAAGCAAAGTGTTCAAGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

873

Amino Acids

95.98

Weight (kDa)

5.92

Isoelectric Point (pI)

50.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011107)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23740 AT4G14120
fragaria_vesca FvH4_4g12980 FvH4_4g12980 FvH4_4g12980 FvH4_4g12980
malus_domestica MD04G1019900.v1.1
prunus_persica Prupe.1G169400_v2.0.a1
pyrus_communis pycom04g01590
rosa_chinensis RchiOBHm_Chr4g0412661
rosa_laevigata RLG00000008269
rosa_multiflora Rmu_co8037358.1_g000001 Rmu_sc0001238.1_g000004
rosa_roxburghii Rroxscaffold_5G00356860
rosa_rugosa Rorug04G0115700
rosa_samantha Rh4AG174900 Rh4BG174300 Rh4CG185800 Rh4DG170800
rosa_wichuraiana Rw4G014660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 931
AasI GACNNNNNNGTC 2 cut(s) 164, 197
Acc36I ACCTGC 2 cut(s) 931, 1987
AccB1I GGYRCC 1 cut(s) 263
AccB7I CCANNNNNTGG 1 cut(s) 671
AccBSI CCGCTC 1 cut(s) 81
AccIII TCCGGA 2 cut(s) 466, 699
AciI CCGC 9 cut(s) 40, 46, 73, 79, 230, 447, 1379, 2204, 2366
AclWI GGATC 6 cut(s) 55, 458, 1692, 1864, 2218, 2231
AcsI RAATTY 5 cut(s) 394, 427, 757, 982, 1828
AcuI CTGAAG 9 cut(s) 635, 832, 990, 1344, 1595, 1635, 2175, 2361, 2490
AcyI GRCGYC 2 cut(s) 252, 264
AfaI GTAC 6 cut(s) 738, 810, 1057, 1456, 1675, 1878
AfiI CCNNNNNNNGG 7 cut(s) 187, 671, 929, 1721, 1990, 1991, 2500
AflII CTTAAG 1 cut(s) 548
AgeI ACCGGT 1 cut(s) 1549
AgsI TTSAA 8 cut(s) 300, 1164, 1336, 1354, 1569, 1670, 2272, 2613
AjnI CCWGG 1 cut(s) 1227
AjuI GAANNNNNNNTTGG 4 cut(s) 136, 168, 935, 967
AleI CACNNNNGTG 1 cut(s) 2066
Alw21I GWGCWC 1 cut(s) 519
Alw26I GTCTC 1 cut(s) 1026
AlwI GGATC 6 cut(s) 55, 458, 1692, 1864, 2218, 2231
AlwNI CAGNNNCTG 2 cut(s) 910, 1388
Ama87I CYCGRG 2 cut(s) 82, 233
Aor13HI TCCGGA 2 cut(s) 466, 699
ApeKI GCWGC 2 cut(s) 48, 2405
ApoI RAATTY 5 cut(s) 394, 427, 757, 982, 1828
AseI ATTAAT 1 cut(s) 1491
AsiGI ACCGGT 1 cut(s) 1549
Asp700I GAANNNNTTC 2 cut(s) 996, 1203
AspLEI GCGC 2 cut(s) 266, 577
AspS9I GGNCC 6 cut(s) 124, 190, 544, 1724, 1823, 1994
AsuC2I CCSGG 2 cut(s) 33, 1593
AsuHPI GGTGA 5 cut(s) 305, 477, 616, 1328, 2455
AvaI CYCGRG 2 cut(s) 82, 233
AvaII GGWCC 6 cut(s) 124, 190, 544, 1724, 1823, 1994
AxyI CCTNAGG 1 cut(s) 492
BaeI ACNNNNGTAYC 4 cut(s) 1080, 1113, 1638, 1671
BamHI GGATCC 1 cut(s) 2223
BanI GGYRCC 1 cut(s) 263
BanII GRGCYC 1 cut(s) 519
BarI GAAGNNNNNNTAC 2 cut(s) 1959, 1991
BauI CACGAG 2 cut(s) 637, 2241
BbsI GAAGAC 1 cut(s) 1481
Bbv12I GWGCWC 1 cut(s) 519
BbvCI CCTCAGC 1 cut(s) 1716
BbvI GCAGC 2 cut(s) 60, 2392
BccI CCATC 8 cut(s) 923, 1031, 1711, 1726, 1814, 2021, 2117, 2356
BceAI ACGGC 2 cut(s) 175, 251
BciT130I CCWGG 1 cut(s) 1229
BciVI GTATCC 3 cut(s) 1081, 1278, 1666
BclI TGATCA 1 cut(s) 901
BcnI CCSGG 2 cut(s) 33, 1593
BcoDI GTCTC 1 cut(s) 1026
BfaI CTAG 4 cut(s) 647, 834, 1721, 1770
BfmI CTRYAG 5 cut(s) 432, 765, 888, 1606, 2530
BfoI RGCGCY 2 cut(s) 267, 578
BfrI CTTAAG 1 cut(s) 548
BfuAI ACCTGC 2 cut(s) 931, 1987
BfuI GTATCC 3 cut(s) 1081, 1278, 1666
BisI GCNGC 3 cut(s) 49, 230, 2406
BlpI GCTNAGC 1 cut(s) 352
BlsI GCNGC 3 cut(s) 50, 231, 2407
BmcAI AGTACT 1 cut(s) 1675
Bme1390I CCNGG 3 cut(s) 33, 1229, 1593
Bme18I GGWCC 6 cut(s) 124, 190, 544, 1724, 1823, 1994
BmeT110I CYCGRG 2 cut(s) 82, 233
BmgT120I GGNCC 6 cut(s) 124, 190, 544, 1724, 1823, 1994
BmiI GGNNCC 6 cut(s) 115, 265, 454, 460, 1995, 2225
BmrFI CCNGG 3 cut(s) 33, 1229, 1593
BmrI ACTGGG 1 cut(s) 1147
BmuI ACTGGG 1 cut(s) 1147
BoxI GACNNNNGTC 1 cut(s) 1464
BpiI GAAGAC 1 cut(s) 1481
BplI GAGNNNNNCTC 2 cut(s) 501, 533
BpmI CTGGAG 1 cut(s) 342
Bpu10I CCTNAGC 1 cut(s) 1716
Bpu1102I GCTNAGC 1 cut(s) 352
BpuMI CCSGG 2 cut(s) 33, 1593
Bsa29I ATCGAT 2 cut(s) 525, 783
BsaBI GATNNNNATC 1 cut(s) 1183
BsaHI GRCGYC 2 cut(s) 252, 264
BsaJI CCNNGG 3 cut(s) 1462, 1592, 2555
BsaWI WCCGGW 6 cut(s) 126, 286, 455, 466, 699, 1549
BsaXI ACNNNNNCTCC 2 cut(s) 1996, 2026
Bsc4I CCNNNNNNNGG 7 cut(s) 187, 671, 929, 1721, 1990, 1991, 2500
Bse118I RCCGGY 1 cut(s) 1549
Bse1I ACTGG 7 cut(s) 115, 1073, 1153, 1659, 1932, 2057, 2135
Bse21I CCTNAGG 1 cut(s) 492
Bse8I GATNNNNATC 1 cut(s) 1183
BseAI TCCGGA 2 cut(s) 466, 699
BseBI CCWGG 1 cut(s) 1229
BseCI ATCGAT 2 cut(s) 525, 783
BseDI CCNNGG 3 cut(s) 1462, 1592, 2555
BseGI GGATG 8 cut(s) 36, 373, 1042, 1108, 1177, 1285, 2212, 2252
BseJI GATNNNNATC 1 cut(s) 1183
BseLI CCNNNNNNNGG 7 cut(s) 187, 671, 929, 1721, 1990, 1991, 2500
BseMII CTCAG 9 cut(s) 366, 483, 491, 809, 1002, 1247, 1380, 1730, 2213
BseNI ACTGG 7 cut(s) 115, 1073, 1153, 1659, 1932, 2057, 2135
BseRI GAGGAG 2 cut(s) 161, 1031
BseXI GCAGC 2 cut(s) 60, 2392
BseYI CCCAGC 1 cut(s) 578
BsgI GTGCAG 1 cut(s) 2245
BshNI GGYRCC 1 cut(s) 263
BshTI ACCGGT 1 cut(s) 1549
BshVI ATCGAT 2 cut(s) 525, 783
BsiHKAI GWGCWC 1 cut(s) 519
BsiHKCI CYCGRG 2 cut(s) 82, 233
BslFI GGGAC 1 cut(s) 1974
BslI CCNNNNNNNGG 7 cut(s) 187, 671, 929, 1721, 1990, 1991, 2500
BsmAI GTCTC 1 cut(s) 1026
BsmFI GGGAC 1 cut(s) 1974
BsmI GAATGC 1 cut(s) 606
BsoBI CYCGRG 2 cut(s) 82, 233
Bsp1286I GDGCHC 1 cut(s) 519
Bsp13I TCCGGA 2 cut(s) 466, 699
Bsp1407I TGTACA 3 cut(s) 736, 808, 1876
Bsp1720I GCTNAGC 1 cut(s) 352
BspACI CCGC 9 cut(s) 40, 46, 73, 79, 230, 447, 1379, 2204, 2366
BspCNI CTCAG 9 cut(s) 365, 484, 490, 808, 1001, 1246, 1381, 1729, 2212
BspDI ATCGAT 2 cut(s) 525, 783
BspEI TCCGGA 2 cut(s) 466, 699
BspHI TCATGA 1 cut(s) 775
BspLI GGNNCC 6 cut(s) 115, 265, 454, 460, 1995, 2225
BspMAI CTGCAG 2 cut(s) 436, 2534
BspMI ACCTGC 2 cut(s) 931, 1987
BspPI GGATC 6 cut(s) 55, 458, 1692, 1864, 2218, 2231
BspQI GCTCTTC 1 cut(s) 1009
BspT107I GGYRCC 1 cut(s) 263
BspTI CTTAAG 1 cut(s) 548
BsrBI CCGCTC 1 cut(s) 81
BsrFI RCCGGY 1 cut(s) 1549
BsrGI TGTACA 3 cut(s) 736, 808, 1876
BsrI ACTGG 7 cut(s) 115, 1073, 1153, 1659, 1932, 2057, 2135
BssAI RCCGGY 1 cut(s) 1549
BssECI CCNNGG 3 cut(s) 1462, 1592, 2555
BssNI GRCGYC 2 cut(s) 252, 264
BssSI CACGAG 2 cut(s) 637, 2241
BssT1I CCWWGG 2 cut(s) 1462, 2555
Bst2BI CACGAG 2 cut(s) 637, 2241
Bst2UI CCWGG 1 cut(s) 1229
Bst4CI ACNGT 7 cut(s) 293, 808, 1678, 2086, 2185, 2495, 2579
Bst6I CTCTTC 4 cut(s) 305, 1009, 1123, 1320
BstACI GRCGYC 2 cut(s) 252, 264
BstAFI CTTAAG 1 cut(s) 548
BstAUI TGTACA 3 cut(s) 736, 808, 1876
BstC8I GCNNGC 3 cut(s) 644, 2455, 2546
BstENI CCTNNNNNAGG 2 cut(s) 185, 1719
BstF5I GGATG 8 cut(s) 36, 373, 1042, 1108, 1177, 1285, 2212, 2252
BstH2I RGCGCY 2 cut(s) 267, 578
BstHHI GCGC 2 cut(s) 266, 577
BstMAI GTCTC 1 cut(s) 1026
BstMWI GCNNNNNNNGC 5 cut(s) 48, 1328, 2303, 2411, 2471
BstNI CCWGG 1 cut(s) 1229
BstNSI RCATGY 2 cut(s) 646, 2544
BstPAI GACNNNNGTC 1 cut(s) 1464
BstSCI CCNGG 3 cut(s) 31, 1227, 1591
BstSFI CTRYAG 5 cut(s) 432, 765, 888, 1606, 2530
BstV1I GCAGC 2 cut(s) 60, 2392
BstV2I GAAGAC 1 cut(s) 1481
BstX2I RGATCY 4 cut(s) 60, 1684, 1856, 2223
BstXI CCANNNNNNTGG 3 cut(s) 145, 1469, 2152
BstYI RGATCY 4 cut(s) 60, 1684, 1856, 2223
Bsu15I ATCGAT 2 cut(s) 525, 783
Bsu36I CCTNAGG 1 cut(s) 492
BsuI GTATCC 3 cut(s) 1081, 1278, 1666
BsuTUI ATCGAT 2 cut(s) 525, 783
BtsCI GGATG 8 cut(s) 36, 373, 1042, 1108, 1177, 1285, 2212, 2252
BtsI GCAGTG 4 cut(s) 881, 1489, 2409, 2527
BveI ACCTGC 2 cut(s) 931, 1987
Cac8I GCNNGC 3 cut(s) 644, 2455, 2546
CaiI CAGNNNCTG 2 cut(s) 910, 1388
CciI TCATGA 1 cut(s) 775
CfoI GCGC 2 cut(s) 266, 577
Cfr10I RCCGGY 1 cut(s) 1549
Cfr13I GGNCC 6 cut(s) 124, 190, 544, 1724, 1823, 1994
ClaI ATCGAT 2 cut(s) 525, 783
CpoI CGGWCCG 1 cut(s) 124
CseI GACGC 1 cut(s) 260
Csp6I GTAC 6 cut(s) 737, 809, 1056, 1455, 1674, 1877
CspAI ACCGGT 1 cut(s) 1549
CspI CGGWCCG 1 cut(s) 124
CviQI GTAC 6 cut(s) 737, 809, 1056, 1455, 1674, 1877
DinI GGCGCC 1 cut(s) 265
DraI TTTAAA 4 cut(s) 571, 1557, 1585, 2436
DrdI GACNNNNNNGTC 2 cut(s) 164, 197
DseDI GACNNNNNNGTC 2 cut(s) 164, 197
Eam1104I CTCTTC 4 cut(s) 305, 1009, 1123, 1320
EarI CTCTTC 4 cut(s) 305, 1009, 1123, 1320
EciI GGCGGA 2 cut(s) 2219, 2381
Ecl136II GAGCTC 1 cut(s) 517
Eco130I CCWWGG 2 cut(s) 1462, 2555
Eco24I GRGCYC 1 cut(s) 519
Eco32I GATATC 2 cut(s) 622, 1442
Eco47I GGWCC 6 cut(s) 124, 190, 544, 1724, 1823, 1994
Eco53kI GAGCTC 1 cut(s) 517
Eco57I CTGAAG 9 cut(s) 635, 832, 990, 1344, 1595, 1635, 2175, 2361, 2490
Eco81I CCTNAGG 1 cut(s) 492
Eco88I CYCGRG 2 cut(s) 82, 233
EcoICRI GAGCTC 1 cut(s) 517
EcoNI CCTNNNNNAGG 2 cut(s) 185, 1719
EcoO109I RGGNCCY 3 cut(s) 190, 1724, 1994
EcoRII CCWGG 1 cut(s) 1227
EcoRV GATATC 2 cut(s) 622, 1442
EcoT14I CCWWGG 2 cut(s) 1462, 2555
EcoT22I ATGCAT 1 cut(s) 1893
EcoT38I GRGCYC 1 cut(s) 519
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
ErhI CCWWGG 2 cut(s) 1462, 2555
FalI AAGNNNNNCTT 2 cut(s) 2211, 2243
FaqI GGGAC 1 cut(s) 1974
FauI CCCGC 2 cut(s) 53, 86
FbaI TGATCA 1 cut(s) 901
Fnu4HI GCNGC 3 cut(s) 49, 230, 2406
FokI GGATG 8 cut(s) 23, 380, 1049, 1115, 1184, 1272, 2219, 2259
FriOI GRGCYC 1 cut(s) 519
Fsp4HI GCNGC 3 cut(s) 49, 230, 2406
FspBI CTAG 4 cut(s) 647, 834, 1721, 1770
GlaI GCGC 2 cut(s) 265, 576
GluI GCNGC 3 cut(s) 49, 230, 2406
GsaI CCCAGC 1 cut(s) 582
GsuI CTGGAG 1 cut(s) 342
HaeII RGCGCY 2 cut(s) 267, 578
HgaI GACGC 1 cut(s) 260
HhaI GCGC 2 cut(s) 266, 577
Hin1I GRCGYC 2 cut(s) 252, 264
Hin6I GCGC 2 cut(s) 264, 575
HinP1I GCGC 2 cut(s) 264, 575
HincII GTYRAC 1 cut(s) 1898
HindII GTYRAC 1 cut(s) 1898
HindIII AAGCTT 1 cut(s) 707
HphI GGTGA 5 cut(s) 305, 477, 616, 1328, 2455
Hpy166II GTNNAC 4 cut(s) 1056, 1898, 1916, 1925
Hpy8I GTNNAC 4 cut(s) 1056, 1898, 1916, 1925
Hpy99I CGWCG 2 cut(s) 161, 254
HpyAV CCTTC 5 cut(s) 493, 610, 680, 1235, 2025
HpyCH4III ACNGT 7 cut(s) 293, 808, 1678, 2086, 2185, 2495, 2579
HpyCH4IV ACGT 1 cut(s) 165
HpyF10VI GCNNNNNNNGC 5 cut(s) 48, 1328, 2303, 2411, 2471
HpySE526I ACGT 1 cut(s) 165
Hsp92I GRCGYC 2 cut(s) 252, 264
HspAI GCGC 2 cut(s) 264, 575
KasI GGCGCC 1 cut(s) 263
Kpn2I TCCGGA 2 cut(s) 466, 699
Ksp22I TGATCA 1 cut(s) 901
LguI GCTCTTC 1 cut(s) 1009
LmnI GCTCC 4 cut(s) 72, 119, 361, 877
Lsp1109I GCAGC 2 cut(s) 60, 2392
MaeI CTAG 4 cut(s) 647, 834, 1721, 1770
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 4 cut(s) 532, 2059, 2194, 2428
MbiI CCGCTC 1 cut(s) 81
MfeI CAATTG 1 cut(s) 1787
MflI RGATCY 4 cut(s) 60, 1684, 1856, 2223
MhlI GDGCHC 1 cut(s) 519
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 8 cut(s) 128, 207, 365, 547, 1028, 1702, 2250, 2283
MmeI TCCRAC 3 cut(s) 102, 180, 1306
Mph1103I ATGCAT 1 cut(s) 1893
MroI TCCGGA 2 cut(s) 466, 699
MroXI GAANNNNTTC 2 cut(s) 996, 1203
MslI CAYNNNNRTG 5 cut(s) 1299, 1500, 1949, 2066, 2245
MspA1I CMGCKG 2 cut(s) 799, 2405
MspCI CTTAAG 1 cut(s) 548
MspR9I CCNGG 3 cut(s) 33, 1229, 1593
MunI CAATTG 1 cut(s) 1787
Mva1269I GAATGC 1 cut(s) 606
MvaI CCWGG 1 cut(s) 1229
MwoI GCNNNNNNNGC 5 cut(s) 48, 1328, 2303, 2411, 2471
NarI GGCGCC 1 cut(s) 264
NciI CCSGG 2 cut(s) 33, 1593
NlaIV GGNNCC 6 cut(s) 115, 265, 454, 460, 1995, 2225
NmuCI GTSAC 2 cut(s) 2059, 2428
NsiI ATGCAT 1 cut(s) 1893
NspI RCATGY 2 cut(s) 646, 2544
OliI CACNNNNGTG 1 cut(s) 2066
PaeI GCATGC 1 cut(s) 646
PagI TCATGA 1 cut(s) 775
PaqCI CACCTGC 1 cut(s) 931
PciSI GCTCTTC 1 cut(s) 1009
PctI GAATGC 1 cut(s) 606
PdmI GAANNNNTTC 2 cut(s) 996, 1203
PflFI GACNNNGTC 1 cut(s) 1946
PflMI CCANNNNNTGG 1 cut(s) 671
PinAI ACCGGT 1 cut(s) 1549
PkrI GCNGC 3 cut(s) 50, 231, 2407
PleI GAGTC 8 cut(s) 127, 206, 365, 547, 1027, 1702, 2250, 2283
PluTI GGCGCC 1 cut(s) 267
PpsI GAGTC 8 cut(s) 127, 206, 365, 547, 1027, 1702, 2250, 2283
PpuMI RGGWCCY 3 cut(s) 190, 1724, 1994
PshAI GACNNNNGTC 1 cut(s) 1464
PshBI ATTAAT 1 cut(s) 1491
Psp124BI GAGCTC 1 cut(s) 519
Psp5II RGGWCCY 3 cut(s) 190, 1724, 1994
Psp6I CCWGG 1 cut(s) 1227
PspFI CCCAGC 1 cut(s) 578
PspGI CCWGG 1 cut(s) 1227
PspN4I GGNNCC 6 cut(s) 115, 265, 454, 460, 1995, 2225
PspPI GGNCC 6 cut(s) 124, 190, 544, 1724, 1823, 1994
PspPPI RGGWCCY 3 cut(s) 190, 1724, 1994
PstI CTGCAG 2 cut(s) 436, 2534
PstNI CAGNNNCTG 2 cut(s) 910, 1388
PsuI RGATCY 4 cut(s) 60, 1684, 1856, 2223
PsyI GACNNNGTC 1 cut(s) 1946
PvuII CAGCTG 2 cut(s) 799, 2405
RsaI GTAC 6 cut(s) 738, 810, 1057, 1456, 1675, 1878
RsaNI GTAC 6 cut(s) 737, 809, 1056, 1455, 1674, 1877
RseI CAYNNNNRTG 5 cut(s) 1299, 1500, 1949, 2066, 2245
Rsr2I CGGWCCG 1 cut(s) 124
RsrII CGGWCCG 1 cut(s) 124
SacI GAGCTC 1 cut(s) 519
SapI GCTCTTC 1 cut(s) 1009
SatI GCNGC 3 cut(s) 49, 230, 2406
Sau96I GGNCC 6 cut(s) 124, 190, 544, 1724, 1823, 1994
ScaI AGTACT 1 cut(s) 1675
SchI GAGTC 8 cut(s) 128, 207, 365, 547, 1028, 1702, 2250, 2283
ScrFI CCNGG 3 cut(s) 33, 1229, 1593
SduI GDGCHC 1 cut(s) 519
SfcI CTRYAG 5 cut(s) 432, 765, 888, 1606, 2530
SfoI GGCGCC 1 cut(s) 265
SinI GGWCC 6 cut(s) 124, 190, 544, 1724, 1823, 1994
SmiMI CAYNNNNRTG 5 cut(s) 1299, 1500, 1949, 2066, 2245
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
SphI GCATGC 1 cut(s) 646
SsiI CCGC 9 cut(s) 40, 46, 73, 79, 230, 447, 1379, 2204, 2366
SspDI GGCGCC 1 cut(s) 263
SspMI CTAG 4 cut(s) 647, 834, 1721, 1770
SstI GAGCTC 1 cut(s) 519
StyD4I CCNGG 3 cut(s) 31, 1227, 1591
StyI CCWWGG 2 cut(s) 1462, 2555
TaaI ACNGT 7 cut(s) 293, 808, 1678, 2086, 2185, 2495, 2579
TaiI ACGT 1 cut(s) 168
TaqI TCGA 7 cut(s) 13, 324, 525, 556, 783, 1706, 2287
TatI WGTACW 6 cut(s) 736, 808, 1055, 1454, 1673, 1876
TauI GCSGC 1 cut(s) 232
TseFI GTSAC 2 cut(s) 2059, 2428
TseI GCWGC 2 cut(s) 48, 2405
Tsp45I GTSAC 2 cut(s) 2059, 2428
TspGWI ACGGA 2 cut(s) 522, 1049
Tth111I GACNNNGTC 1 cut(s) 1946
Van91I CCANNNNNTGG 1 cut(s) 671
Vha464I CTTAAG 1 cut(s) 548
VpaK11BI GGWCC 6 cut(s) 124, 190, 544, 1724, 1823, 1994
VspI ATTAAT 1 cut(s) 1491
XagI CCTNNNNNAGG 2 cut(s) 185, 1719
XapI RAATTY 5 cut(s) 394, 427, 757, 982, 1828
XceI RCATGY 2 cut(s) 646, 2544
XmnI GAANNNNTTC 2 cut(s) 996, 1203
XspI CTAG 4 cut(s) 647, 834, 1721, 1770
ZrmI AGTACT 1 cut(s) 1675
Zsp2I ATGCAT 1 cut(s) 1893
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.