FvH4_4g13700

The GINS complex plays an essential role in the initiation of DNA replication

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006170
Physical Location & Seq
Forward (+)
17235295 .. 17237718
2424 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g13700.t2

Sequence Viewer

Length: 693 bp
ATGGACGGGAGAGACGGAGAAGGGTCAACGGAGGAATACGAGACGTTGATTCAGACGACTGATGTGGAGCTTCTGAAGAGGGCGTGGCGGCAGGAGAAGGCGGCGCCGGAGATTTTGAAATTCGAGACTGATTTGATTGCCAGGCTCACCGGTCAGATCGAATTGGTGGAAGAAAATGTGGAGCAAGATGCTCAGTCTGGTGTTGATCCACTTAATGTATCACTCTACCAGATGGATTTGGACAGGACTCAATTCTTGTTGAGATCTTATCTCCGGATTCGGCTGCAGAAGATTGAGAAATACATCTTTCACATCTTAGCCACTGCAGAACTCTACAACCGTCTCTCTAAACAAGAGAAGGCATTTGCAAAAACGTGTCTTGTTGATTTGGAGAAGCATCTTGAAGAGAGTGTTCTGTTGAAATTGCCTAATAACTACCAGTCTATATTCAAGCAATCCATGATCAGTGAAGAGAATGACATGGTGGCAAAACCTCAGTTAGACATGTTTGTTGTATGCAAAACAAATTACTATCTTGGACATATCCAGCTTGAGGACAATGAAGATGGACCATCAGAGGTAAATCAGAGACCTTTGGAGGAACCTTTCGAAATGGAGCCTGATGTTTTATACTTTGTGCGTTATAAAGCTATAAAGAGATTCGTAGAGGAAGGGAGGATTGATTTATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SLD5_C Sld5

Protein Analysis

231

Amino Acids

26.99

Weight (kDa)

4.7

Isoelectric Point (pI)

54.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sld5 PF05916 71 - 136 8.8e-12 GINS complex protein helical bundle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 645
AccB1I GGYRCC 1 cut(s) 103
AccIII TCCGGA 1 cut(s) 273
AciI CCGC 2 cut(s) 88, 101
AclWI GGATC 1 cut(s) 200
AcsI RAATTY 1 cut(s) 119
AcuI CTGAAG 1 cut(s) 95
AcyI GRCGYC 1 cut(s) 104
AfiI CCNNNNNNNGG 1 cut(s) 553
AflIII ACRYGT 2 cut(s) 374, 504
AgeI ACCGGT 1 cut(s) 149
AgsI TTSAA 4 cut(s) 118, 404, 421, 451
AjnI CCWGG 1 cut(s) 140
AluBI AGCT 3 cut(s) 70, 550, 650
AluI AGCT 3 cut(s) 70, 550, 650
Alw26I GTCTC 5 cut(s) 6, 35, 119, 347, 583
AlwI GGATC 1 cut(s) 200
Aor13HI TCCGGA 1 cut(s) 273
ApeKI GCWGC 1 cut(s) 283
ApoI RAATTY 1 cut(s) 119
AsiGI ACCGGT 1 cut(s) 149
AspLEI GCGC 1 cut(s) 106
AspS9I GGNCC 1 cut(s) 569
AsuHPI GGTGA 1 cut(s) 139
AsuII TTCGAA 1 cut(s) 609
AvaII GGWCC 1 cut(s) 569
BanI GGYRCC 1 cut(s) 103
BbvI GCAGC 1 cut(s) 270
BccI CCATC 3 cut(s) 226, 560, 580
BciT130I CCWGG 1 cut(s) 142
BclI TGATCA 1 cut(s) 462
BcoDI GTCTC 5 cut(s) 6, 35, 119, 347, 583
BfmI CTRYAG 2 cut(s) 284, 324
BfoI RGCGCY 1 cut(s) 107
BglII AGATCT 1 cut(s) 263
BisI GCNGC 3 cut(s) 89, 102, 284
BlsI GCNGC 3 cut(s) 90, 103, 285
Bme1390I CCNGG 1 cut(s) 142
Bme18I GGWCC 1 cut(s) 569
BmgT120I GGNCC 1 cut(s) 569
BmiI GGNNCC 3 cut(s) 105, 603, 618
BmrFI CCNGG 1 cut(s) 142
BmsI GCATC 2 cut(s) 178, 406
Bpu14I TTCGAA 1 cut(s) 609
BpuEI CTTGAG 1 cut(s) 572
BsaHI GRCGYC 1 cut(s) 104
BsaI GGTCTC 1 cut(s) 583
BsaWI WCCGGW 2 cut(s) 149, 273
Bsc4I CCNNNNNNNGG 1 cut(s) 553
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 1 cut(s) 439
BseAI TCCGGA 1 cut(s) 273
BseBI CCWGG 1 cut(s) 142
BseLI CCNNNNNNNGG 1 cut(s) 553
BseMII CTCAG 2 cut(s) 206, 509
BseNI ACTGG 1 cut(s) 439
BseXI GCAGC 1 cut(s) 270
BshNI GGYRCC 1 cut(s) 103
BshTI ACCGGT 1 cut(s) 149
BsiSI CCGG 3 cut(s) 107, 150, 274
BslI CCNNNNNNNGG 1 cut(s) 553
BsmAI GTCTC 5 cut(s) 6, 35, 119, 347, 583
BsmBI CGTCTC 3 cut(s) 6, 35, 347
Bso31I GGTCTC 1 cut(s) 583
Bsp119I TTCGAA 1 cut(s) 609
Bsp13I TCCGGA 1 cut(s) 273
Bsp143I GATC 4 cut(s) 156, 205, 263, 462
BspACI CCGC 2 cut(s) 88, 101
BspCNI CTCAG 2 cut(s) 205, 508
BspEI TCCGGA 1 cut(s) 273
BspLI GGNNCC 3 cut(s) 105, 603, 618
BspMAI CTGCAG 2 cut(s) 288, 328
BspPI GGATC 1 cut(s) 200
BspT104I TTCGAA 1 cut(s) 609
BspT107I GGYRCC 1 cut(s) 103
BspTNI GGTCTC 1 cut(s) 583
BsrFI RCCGGY 1 cut(s) 149
BsrI ACTGG 1 cut(s) 439
BssAI RCCGGY 1 cut(s) 149
BssMI GATC 4 cut(s) 156, 205, 263, 462
BssNI GRCGYC 1 cut(s) 104
Bst2UI CCWGG 1 cut(s) 142
Bst4CI ACNGT 1 cut(s) 341
Bst6I CTCTTC 3 cut(s) 71, 399, 465
BstACI GRCGYC 1 cut(s) 104
BstBI TTCGAA 1 cut(s) 609
BstDEI CTNAG 3 cut(s) 192, 316, 495
BstH2I RGCGCY 1 cut(s) 107
BstHHI GCGC 1 cut(s) 106
BstKTI GATC 4 cut(s) 159, 208, 266, 465
BstMAI GTCTC 5 cut(s) 6, 35, 119, 347, 583
BstMBI GATC 4 cut(s) 156, 205, 263, 462
BstNI CCWGG 1 cut(s) 142
BstNSI RCATGY 1 cut(s) 508
BstSCI CCNGG 1 cut(s) 140
BstSFI CTRYAG 2 cut(s) 284, 324
BstV1I GCAGC 1 cut(s) 270
BstX2I RGATCY 1 cut(s) 263
BstYI RGATCY 1 cut(s) 263
BtsI GCAGTG 1 cut(s) 321
BtsIMutI CAGTG 2 cut(s) 321, 472
CfoI GCGC 1 cut(s) 106
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 569
CspAI ACCGGT 1 cut(s) 149
CviAII CATG 3 cut(s) 460, 481, 505
CviJI RGCY 7 cut(s) 70, 145, 283, 320, 550, 619, 650
CviKI_1 RGCY 7 cut(s) 70, 145, 283, 320, 550, 619, 650
DdeI CTNAG 3 cut(s) 192, 316, 495
DinI GGCGCC 1 cut(s) 105
DpnI GATC 4 cut(s) 158, 207, 265, 464
DpnII GATC 4 cut(s) 156, 205, 263, 462
Eam1104I CTCTTC 3 cut(s) 71, 399, 465
EarI CTCTTC 3 cut(s) 71, 399, 465
Eco31I GGTCTC 1 cut(s) 583
Eco47I GGWCC 1 cut(s) 569
Eco57I CTGAAG 1 cut(s) 95
EcoRII CCWGG 1 cut(s) 140
EgeI GGCGCC 1 cut(s) 105
EheI GGCGCC 1 cut(s) 105
Esp3I CGTCTC 3 cut(s) 6, 35, 347
FaeI CATG 3 cut(s) 463, 484, 508
FaiI YATR 9 cut(s) 446, 461, 482, 506, 517, 543, 631, 645, 653
FatI CATG 3 cut(s) 459, 480, 504
FbaI TGATCA 1 cut(s) 462
Fnu4HI GCNGC 3 cut(s) 89, 102, 284
Fsp4HI GCNGC 3 cut(s) 89, 102, 284
GlaI GCGC 1 cut(s) 105
GluI GCNGC 3 cut(s) 89, 102, 284
HaeII RGCGCY 1 cut(s) 107
HapII CCGG 3 cut(s) 107, 150, 274
HhaI GCGC 1 cut(s) 106
Hin1I GRCGYC 1 cut(s) 104
Hin1II CATG 3 cut(s) 463, 484, 508
Hin6I GCGC 1 cut(s) 104
HinP1I GCGC 1 cut(s) 104
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HinfI GANTC 4 cut(s) 49, 247, 277, 660
HpaII CCGG 3 cut(s) 107, 150, 274
HphI GGTGA 1 cut(s) 139
Hpy166II GTNNAC 1 cut(s) 27
Hpy188I TCNGA 6 cut(s) 54, 75, 156, 577, 588, 692
Hpy188III TCNNGA 3 cut(s) 124, 274, 401
Hpy8I GTNNAC 1 cut(s) 27
HpyAV CCTTC 4 cut(s) 14, 91, 352, 665
HpyCH4III ACNGT 1 cut(s) 341
HpyCH4IV ACGT 2 cut(s) 44, 374
HpyCH4V TGCA 4 cut(s) 286, 326, 368, 519
HpyF3I CTNAG 3 cut(s) 192, 316, 495
HpySE526I ACGT 2 cut(s) 44, 374
Hsp92I GRCGYC 1 cut(s) 104
Hsp92II CATG 3 cut(s) 463, 484, 508
HspAI GCGC 1 cut(s) 104
KasI GGCGCC 1 cut(s) 103
Kpn2I TCCGGA 1 cut(s) 273
Ksp22I TGATCA 1 cut(s) 462
Kzo9I GATC 4 cut(s) 156, 205, 263, 462
LmnI GCTCC 3 cut(s) 67, 181, 616
Lsp1109I GCAGC 1 cut(s) 270
LweI GCATC 2 cut(s) 178, 406
MaeII ACGT 2 cut(s) 44, 374
MalI GATC 4 cut(s) 158, 207, 265, 464
MboI GATC 4 cut(s) 156, 205, 263, 462
MboII GAAGA 6 cut(s) 88, 182, 301, 416, 482, 575
MflI RGATCY 1 cut(s) 263
MluCI AATT 5 cut(s) 119, 161, 251, 422, 526
Mly113I GGCGCC 1 cut(s) 104
MlyI GAGTC 1 cut(s) 241
MnlI CCTC 8 cut(s) 25, 72, 504, 547, 571, 592, 661, 669
MroI TCCGGA 1 cut(s) 273
MseI TTAA 1 cut(s) 213
MspI CCGG 3 cut(s) 107, 150, 274
MspR9I CCNGG 1 cut(s) 142
MvaI CCWGG 1 cut(s) 142
NarI GGCGCC 1 cut(s) 104
NdeII GATC 4 cut(s) 156, 205, 263, 462
NlaIII CATG 3 cut(s) 463, 484, 508
NlaIV GGNNCC 3 cut(s) 105, 603, 618
NspI RCATGY 1 cut(s) 508
NspV TTCGAA 1 cut(s) 609
PciI ACATGT 1 cut(s) 504
PfeI GAWTC 3 cut(s) 49, 277, 660
PinAI ACCGGT 1 cut(s) 149
PkrI GCNGC 3 cut(s) 90, 103, 285
PleI GAGTC 1 cut(s) 241
PluTI GGCGCC 1 cut(s) 107
PpsI GAGTC 1 cut(s) 241
PscI ACATGT 1 cut(s) 504
PsiI TTATAA 1 cut(s) 645
Psp6I CCWGG 1 cut(s) 140
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 3 cut(s) 105, 603, 618
PspPI GGNCC 1 cut(s) 569
PstI CTGCAG 2 cut(s) 288, 328
PsuI RGATCY 1 cut(s) 263
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 3 cut(s) 89, 102, 284
Sau3AI GATC 4 cut(s) 156, 205, 263, 462
Sau96I GGNCC 1 cut(s) 569
SchI GAGTC 1 cut(s) 241
ScrFI CCNGG 1 cut(s) 142
SetI ASST 9 cut(s) 47, 72, 377, 496, 552, 582, 595, 607, 652
SfaNI GCATC 2 cut(s) 178, 406
SfcI CTRYAG 2 cut(s) 284, 324
SfoI GGCGCC 1 cut(s) 105
SfuI TTCGAA 1 cut(s) 609
SinI GGWCC 1 cut(s) 569
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
Sse9I AATT 5 cut(s) 119, 161, 251, 422, 526
SsiI CCGC 2 cut(s) 88, 101
SspDI GGCGCC 1 cut(s) 103
StyD4I CCNGG 1 cut(s) 140
TaaI ACNGT 1 cut(s) 341
TaiI ACGT 2 cut(s) 47, 377
TaqI TCGA 3 cut(s) 123, 159, 609
TasI AATT 5 cut(s) 119, 161, 251, 422, 526
TauI GCSGC 2 cut(s) 91, 104
TfiI GAWTC 3 cut(s) 49, 277, 660
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 2 cut(s) 328, 472
TseI GCWGC 1 cut(s) 283
TspDTI ATGAA 1 cut(s) 576
TspGWI ACGGA 2 cut(s) 30, 44
TspRI CASTG 2 cut(s) 328, 472
VpaK11BI GGWCC 1 cut(s) 569
XapI RAATTY 1 cut(s) 119
XceI RCATGY 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.