MD16G1152100.v1.1

The GINS complex plays an essential role in the initiation of DNA replication

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006170
Physical Location & Seq
Forward (+)
12003340 .. 12005239
1900 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1152100.v1.1.491

Sequence Viewer

Length: 549 bp
ATGGATTCGGGTCTGGAATCAGGGTCAACGGAGGATTACGAGACGGACGTGAAGCTCCTGAAGAGTGCGTGGCGGCAGGAGAAGGCCACTCCGGAGGTTCTTCAGTTCGAGAGTGCCTTGATTCGGAGAATCATTGAGCAGATCCAATTGATGGAAGAAACTGTGGAGGAGTTCACTGAGAGCGGTATTGATCCGCTTACCGTGTCACTCTACCAGATGGATTTGGACAGGACTCAGTTTTTGTTGAGATCTTATTACCGAATTCGTTTGCAGAAGATTGAGAAATACATGTTTCACATTCGGGATAATGCTGAAAGCCGTCTCTATGATAAGGAGAAGCTGTTTGTTGAACAGCGAGGACTCCAACTATCTCATTTTTATGGTTGCAGGTGCTGCGCTGATTTGCAGACGCATCTTGAAAAGAGCGTTCTGTTGCAGTTGCCTTCTACTTATCAGTCTGTATTCCGTCAATCAAGTGAAAAGGAGACTGACATGGAATCGTATATGGTCGCTATTGTGCTAAGAAAAACTTGTAATAGCATGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SLD5_C Sld5

Protein Analysis

183

Amino Acids

21.51

Weight (kDa)

5.12

Isoelectric Point (pI)

63.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sld5 PF05916 65 - 105 1.4e-07 GINS complex protein helical bundle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 378
Acc36I ACCTGC 1 cut(s) 378
AccB7I CCANNNNNTGG 1 cut(s) 151
AccBSI CCGCTC 1 cut(s) 183
AccIII TCCGGA 1 cut(s) 91
AciI CCGC 3 cut(s) 73, 183, 194
AclWI GGATC 2 cut(s) 136, 185
AcsI RAATTY 1 cut(s) 261
AcuI CTGAAG 2 cut(s) 80, 86
AfiI CCNNNNNNNGG 2 cut(s) 123, 151
AflIII ACRYGT 1 cut(s) 288
AgsI TTSAA 2 cut(s) 350, 419
AjiI CACGTC 1 cut(s) 49
AluBI AGCT 2 cut(s) 55, 340
AluI AGCT 2 cut(s) 55, 340
Alw26I GTCTC 3 cut(s) 35, 326, 479
AlwI GGATC 2 cut(s) 136, 185
AlwNI CAGNNNCTG 1 cut(s) 393
Aor13HI TCCGGA 1 cut(s) 91
AoxI GGCC 1 cut(s) 84
ApeKI GCWGC 1 cut(s) 393
ApoI RAATTY 1 cut(s) 261
ArsI GACNNNNNNTTYG 2 cut(s) 223, 255
AspLEI GCGC 1 cut(s) 398
BbvI GCAGC 1 cut(s) 380
BccI CCATC 2 cut(s) 145, 211
BceAI ACGGC 1 cut(s) 303
BcoDI GTCTC 3 cut(s) 35, 326, 479
BfuAI ACCTGC 1 cut(s) 378
BglII AGATCT 1 cut(s) 248
BisI GCNGC 2 cut(s) 74, 394
BlsI GCNGC 2 cut(s) 75, 395
BmgBI CACGTC 1 cut(s) 49
BmsI GCATC 1 cut(s) 421
BsaWI WCCGGW 1 cut(s) 91
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 151
BseAI TCCGGA 1 cut(s) 91
BseLI CCNNNNNNNGG 2 cut(s) 123, 151
BseMII CTCAG 2 cut(s) 168, 248
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 1 cut(s) 380
BshFI GGCC 1 cut(s) 86
BsiSI CCGG 1 cut(s) 92
BslI CCNNNNNNNGG 2 cut(s) 123, 151
BsmAI GTCTC 3 cut(s) 35, 326, 479
BsmBI CGTCTC 2 cut(s) 35, 326
BsnI GGCC 1 cut(s) 86
Bsp13I TCCGGA 1 cut(s) 91
Bsp143I GATC 3 cut(s) 141, 190, 248
BspACI CCGC 3 cut(s) 73, 183, 194
BspANI GGCC 1 cut(s) 86
BspCNI CTCAG 2 cut(s) 169, 247
BspEI TCCGGA 1 cut(s) 91
BspMI ACCTGC 1 cut(s) 378
BspPI GGATC 2 cut(s) 136, 185
BsrBI CCGCTC 1 cut(s) 183
BssMI GATC 3 cut(s) 141, 190, 248
Bst4CI ACNGT 2 cut(s) 163, 202
Bst6I CTCTTC 1 cut(s) 56
BstAPI GCANNNNNTGC 1 cut(s) 393
BstC8I GCNNGC 1 cut(s) 542
BstDEI CTNAG 3 cut(s) 177, 234, 521
BstHHI GCGC 1 cut(s) 398
BstKTI GATC 3 cut(s) 144, 193, 251
BstMAI GTCTC 3 cut(s) 35, 326, 479
BstMBI GATC 3 cut(s) 141, 190, 248
BstMWI GCNNNNNNNGC 1 cut(s) 393
BstNSI RCATGY 2 cut(s) 292, 544
BstV1I GCAGC 1 cut(s) 380
BstX2I RGATCY 2 cut(s) 141, 248
BstYI RGATCY 2 cut(s) 141, 248
BsuRI GGCC 1 cut(s) 86
BtrI CACGTC 1 cut(s) 49
BtsIMutI CAGTG 1 cut(s) 174
BveI ACCTGC 1 cut(s) 378
Cac8I GCNNGC 1 cut(s) 542
CaiI CAGNNNCTG 1 cut(s) 393
CfoI GCGC 1 cut(s) 398
CseI GACGC 1 cut(s) 418
CviAII CATG 3 cut(s) 289, 493, 541
CviJI RGCY 4 cut(s) 55, 86, 318, 340
CviKI_1 RGCY 4 cut(s) 55, 86, 318, 340
DdeI CTNAG 3 cut(s) 177, 234, 521
DpnI GATC 3 cut(s) 143, 192, 250
DpnII GATC 3 cut(s) 141, 190, 248
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Eco57I CTGAAG 2 cut(s) 80, 86
EcoRI GAATTC 1 cut(s) 261
Esp3I CGTCTC 2 cut(s) 35, 326
FaeI CATG 3 cut(s) 292, 496, 544
FaiI YATR 7 cut(s) 290, 327, 381, 494, 504, 506, 542
FalI AAGNNNNNCTT 2 cut(s) 514, 546
FatI CATG 3 cut(s) 288, 492, 540
Fnu4HI GCNGC 2 cut(s) 74, 394
Fsp4HI GCNGC 2 cut(s) 74, 394
GlaI GCGC 1 cut(s) 397
GluI GCNGC 2 cut(s) 74, 394
HaeIII GGCC 1 cut(s) 86
HapII CCGG 1 cut(s) 92
HgaI GACGC 1 cut(s) 418
HhaI GCGC 1 cut(s) 398
Hin1II CATG 3 cut(s) 292, 496, 544
Hin6I GCGC 1 cut(s) 396
HinP1I GCGC 1 cut(s) 396
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HinfI GANTC 7 cut(s) 5, 17, 121, 129, 232, 360, 497
HpaII CCGG 1 cut(s) 92
Hpy166II GTNNAC 2 cut(s) 27, 174
Hpy188I TCNGA 1 cut(s) 126
Hpy188III TCNNGA 6 cut(s) 14, 58, 92, 109, 302, 416
Hpy8I GTNNAC 2 cut(s) 27, 174
HpyAV CCTTC 2 cut(s) 76, 453
HpyCH4III ACNGT 2 cut(s) 163, 202
HpyCH4IV ACGT 1 cut(s) 48
HpyCH4V TGCA 5 cut(s) 271, 387, 406, 436, 544
HpyF10VI GCNNNNNNNGC 1 cut(s) 393
HpyF3I CTNAG 3 cut(s) 177, 234, 521
HpySE526I ACGT 1 cut(s) 48
Hsp92II CATG 3 cut(s) 292, 496, 544
HspAI GCGC 1 cut(s) 396
Kpn2I TCCGGA 1 cut(s) 91
Kzo9I GATC 3 cut(s) 141, 190, 248
LmnI GCTCC 1 cut(s) 60
LpnPI CCDG 7 cut(s) 6, 62, 71, 105, 214, 227, 373
Lsp1109I GCAGC 1 cut(s) 380
LweI GCATC 1 cut(s) 421
MaeII ACGT 1 cut(s) 48
MaeIII GTNAC 1 cut(s) 204
MalI GATC 3 cut(s) 143, 192, 250
MbiI CCGCTC 1 cut(s) 183
MboI GATC 3 cut(s) 141, 190, 248
MboII GAAGA 4 cut(s) 73, 92, 167, 286
MfeI CAATTG 1 cut(s) 146
MflI RGATCY 2 cut(s) 141, 248
MluCI AATT 2 cut(s) 146, 261
MlyI GAGTC 2 cut(s) 226, 354
MmeI TCCRAC 1 cut(s) 388
MnlI CCTC 4 cut(s) 25, 88, 160, 350
MroI TCCGGA 1 cut(s) 91
MslI CAYNNNNRTG 1 cut(s) 378
MspI CCGG 1 cut(s) 92
MunI CAATTG 1 cut(s) 146
MwoI GCNNNNNNNGC 1 cut(s) 393
NdeII GATC 3 cut(s) 141, 190, 248
NlaIII CATG 3 cut(s) 292, 496, 544
NmuCI GTSAC 1 cut(s) 204
NspI RCATGY 2 cut(s) 292, 544
PaeI GCATGC 1 cut(s) 544
PaqCI CACCTGC 1 cut(s) 378
PciI ACATGT 1 cut(s) 288
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PfeI GAWTC 5 cut(s) 5, 17, 121, 129, 497
PflMI CCANNNNNTGG 1 cut(s) 151
PkrI GCNGC 2 cut(s) 75, 395
PleI GAGTC 2 cut(s) 226, 354
PpsI GAGTC 2 cut(s) 226, 354
PscI ACATGT 1 cut(s) 288
PstNI CAGNNNCTG 1 cut(s) 393
PsuI RGATCY 2 cut(s) 141, 248
RseI CAYNNNNRTG 1 cut(s) 378
SatI GCNGC 2 cut(s) 74, 394
Sau3AI GATC 3 cut(s) 141, 190, 248
SchI GAGTC 2 cut(s) 226, 354
SetI ASST 5 cut(s) 51, 57, 99, 342, 392
SfaNI GCATC 1 cut(s) 421
SmiMI CAYNNNNRTG 1 cut(s) 378
SphI GCATGC 1 cut(s) 544
Sse9I AATT 2 cut(s) 146, 261
SsiI CCGC 3 cut(s) 73, 183, 194
TaaI ACNGT 2 cut(s) 163, 202
TaiI ACGT 1 cut(s) 51
TaqI TCGA 1 cut(s) 108
TasI AATT 2 cut(s) 146, 261
TauI GCSGC 1 cut(s) 76
TfiI GAWTC 5 cut(s) 5, 17, 121, 129, 497
TscAI CASTG 1 cut(s) 181
TseFI GTSAC 1 cut(s) 204
TseI GCWGC 1 cut(s) 393
Tsp45I GTSAC 1 cut(s) 204
TspGWI ACGGA 3 cut(s) 44, 59, 455
TspRI CASTG 1 cut(s) 181
Van91I CCANNNNNTGG 1 cut(s) 151
XapI RAATTY 1 cut(s) 261
XceI RCATGY 2 cut(s) 292, 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.