RchiOBHm_Chr6g0255221

The GINS complex plays an essential role in the initiation of DNA replication

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0006170
Physical Location & Seq
Reverse (-)
10568871 .. 10569599
729 bp
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UTR
Exon/CDS
Intron
PRQ22893

Sequence Viewer

Length: 351 bp
ATGGATTTAGACAGGTCTAAGTTCTTATTGAGATCTTATCTTCGAATTCGGCTGCAGAAGATTGAGAAATACATCTTTCACATCTTAGCTACTGCTAAACTCTACAACCGTCTCTCCAAACAGGAGAAGGCATTTGCAAAAACTGTTCTGTTGAAATTGCCTAATAAATATCAGTCTATATTCAAGCAATCCATGATCAGTGAAGAGAATGACATGGGTACTCATATTATCTTCTTTCCACATTTCATACATCTCTGTGTTTTCCGTTTCACACATGTGTGTGTGTGTTTTGGTATTTATCAACTTCGTTTTTCTAAAAATGTTGTGGCCAGTGCTCTAGTCAATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SLD5_C Sld5

Protein Analysis

116

Amino Acids

13.73

Weight (kDa)

9.92

Isoelectric Point (pI)

53.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sld5 PF05916 1 - 58 2.1e-09 GINS complex protein helical bundle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 327
AcsI RAATTY 1 cut(s) 45
AfaI GTAC 1 cut(s) 220
AflIII ACRYGT 1 cut(s) 274
AgsI TTSAA 2 cut(s) 154, 184
AleI CACNNNNGTG 2 cut(s) 275, 277
AluBI AGCT 1 cut(s) 89
AluI AGCT 1 cut(s) 89
Alw21I GWGCWC 1 cut(s) 337
Alw26I GTCTC 1 cut(s) 116
AoxI GGCC 1 cut(s) 327
ApeKI GCWGC 1 cut(s) 52
ApoI RAATTY 1 cut(s) 45
AsuII TTCGAA 1 cut(s) 43
BalI TGGCCA 1 cut(s) 329
Bbv12I GWGCWC 1 cut(s) 337
BbvI GCAGC 1 cut(s) 39
BclI TGATCA 1 cut(s) 195
BcoDI GTCTC 1 cut(s) 116
BfaI CTAG 1 cut(s) 338
BfmI CTRYAG 1 cut(s) 53
BglII AGATCT 1 cut(s) 32
BisI GCNGC 1 cut(s) 53
BlsI GCNGC 1 cut(s) 54
Bpu14I TTCGAA 1 cut(s) 43
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bse1I ACTGG 1 cut(s) 330
BseNI ACTGG 1 cut(s) 330
BseXI GCAGC 1 cut(s) 39
BshFI GGCC 1 cut(s) 329
BsiHKAI GWGCWC 1 cut(s) 337
BsmAI GTCTC 1 cut(s) 116
BsmBI CGTCTC 1 cut(s) 116
BsnI GGCC 1 cut(s) 329
Bsp119I TTCGAA 1 cut(s) 43
Bsp1286I GDGCHC 1 cut(s) 337
Bsp143I GATC 2 cut(s) 32, 195
BspANI GGCC 1 cut(s) 329
BspMAI CTGCAG 1 cut(s) 57
BspT104I TTCGAA 1 cut(s) 43
BsrI ACTGG 1 cut(s) 330
BssMI GATC 2 cut(s) 32, 195
Bst4CI ACNGT 2 cut(s) 110, 145
Bst6I CTCTTC 1 cut(s) 198
BstBI TTCGAA 1 cut(s) 43
BstDEI CTNAG 3 cut(s) 18, 85, 348
BstKTI GATC 2 cut(s) 35, 198
BstMAI GTCTC 1 cut(s) 116
BstMBI GATC 2 cut(s) 32, 195
BstNSI RCATGY 1 cut(s) 278
BstSFI CTRYAG 1 cut(s) 53
BstV1I GCAGC 1 cut(s) 39
BstX2I RGATCY 1 cut(s) 32
BstYI RGATCY 1 cut(s) 32
BsuRI GGCC 1 cut(s) 329
BtsIMutI CAGTG 2 cut(s) 205, 337
Csp6I GTAC 1 cut(s) 219
CviAII CATG 3 cut(s) 193, 214, 275
CviJI RGCY 3 cut(s) 52, 89, 329
CviKI_1 RGCY 3 cut(s) 52, 89, 329
CviQI GTAC 1 cut(s) 219
DdeI CTNAG 3 cut(s) 18, 85, 348
DpnI GATC 2 cut(s) 34, 197
DpnII GATC 2 cut(s) 32, 195
EaeI YGGCCR 1 cut(s) 327
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
EcoRI GAATTC 1 cut(s) 45
Esp3I CGTCTC 1 cut(s) 116
FaeI CATG 3 cut(s) 196, 217, 278
FaiI YATR 6 cut(s) 179, 194, 215, 225, 248, 276
FatI CATG 3 cut(s) 192, 213, 274
FbaI TGATCA 1 cut(s) 195
Fnu4HI GCNGC 1 cut(s) 53
Fsp4HI GCNGC 1 cut(s) 53
FspBI CTAG 1 cut(s) 338
GluI GCNGC 1 cut(s) 53
HaeIII GGCC 1 cut(s) 329
Hin1II CATG 3 cut(s) 196, 217, 278
HpyAV CCTTC 1 cut(s) 121
HpyCH4III ACNGT 2 cut(s) 110, 145
HpyCH4V TGCA 2 cut(s) 55, 137
HpyF3I CTNAG 3 cut(s) 18, 85, 348
Hsp92II CATG 3 cut(s) 196, 217, 278
Ksp22I TGATCA 1 cut(s) 195
Kzo9I GATC 2 cut(s) 32, 195
LpnPI CCDG 2 cut(s) 107, 343
Lsp1109I GCAGC 1 cut(s) 39
MaeI CTAG 1 cut(s) 338
MalI GATC 2 cut(s) 34, 197
MboI GATC 2 cut(s) 32, 195
MboII GAAGA 4 cut(s) 32, 70, 215, 223
MflI RGATCY 1 cut(s) 32
MhlI GDGCHC 1 cut(s) 337
MlsI TGGCCA 1 cut(s) 329
MluCI AATT 2 cut(s) 45, 155
MluNI TGGCCA 1 cut(s) 329
Mox20I TGGCCA 1 cut(s) 329
MscI TGGCCA 1 cut(s) 329
MslI CAYNNNNRTG 4 cut(s) 255, 275, 277, 279
Msp20I TGGCCA 1 cut(s) 329
NdeII GATC 2 cut(s) 32, 195
NlaIII CATG 3 cut(s) 196, 217, 278
NspI RCATGY 1 cut(s) 278
NspV TTCGAA 1 cut(s) 43
OliI CACNNNNGTG 2 cut(s) 275, 277
PciI ACATGT 1 cut(s) 274
PkrI GCNGC 1 cut(s) 54
PscI ACATGT 1 cut(s) 274
PstI CTGCAG 1 cut(s) 57
PsuI RGATCY 1 cut(s) 32
RsaI GTAC 1 cut(s) 220
RsaNI GTAC 1 cut(s) 219
RseI CAYNNNNRTG 4 cut(s) 255, 275, 277, 279
SatI GCNGC 1 cut(s) 53
Sau3AI GATC 2 cut(s) 32, 195
SduI GDGCHC 1 cut(s) 337
SetI ASST 2 cut(s) 17, 91
SfcI CTRYAG 1 cut(s) 53
SfuI TTCGAA 1 cut(s) 43
SgeI CNNG 7 cut(s) 25, 134, 196, 205, 226, 287, 342
SmiMI CAYNNNNRTG 4 cut(s) 255, 275, 277, 279
Sse9I AATT 2 cut(s) 45, 155
SspMI CTAG 1 cut(s) 338
TaaI ACNGT 2 cut(s) 110, 145
TaqI TCGA 1 cut(s) 43
TasI AATT 2 cut(s) 45, 155
TscAI CASTG 2 cut(s) 205, 337
TseI GCWGC 1 cut(s) 52
TspDTI ATGAA 1 cut(s) 235
TspGWI ACGGA 1 cut(s) 254
TspRI CASTG 2 cut(s) 205, 337
XapI RAATTY 1 cut(s) 45
XceI RCATGY 1 cut(s) 278
XspI CTAG 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.