RLG00000031854

The GINS complex plays an essential role in the initiation of DNA replication

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0006170
Physical Location & Seq
Reverse (-)
8779400 .. 8780735
1336 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031854

Sequence Viewer

Length: 414 bp
ATGGAGCAACTAACCTCGAGGGCGAGCAACCGTATCATCCCGCTCCTTAAGACCCTGAGGACCTCCCTCCTCTTCGTCCACACCGTCTTCCTCTCGCTGCCTGTCGGAATCGCTCTCAAAATGGATTTGGACAGGTCTCAGTTCTTATTGAGATCTTATCTTCGAATTCTGCTGCAGAAGATTGAGAAATACATCTTTCACATCTTAGCTACTGCTGAACTCTACAACCGTCTCTCCAAACAGGAGAAGGCATTTGCAAAAACGTGTCTTGTTGATTTGGAGAAGCATCTTGAAGAGAGTGTTCTGTTGAAATTGCTTAATAACTATCAGTCTATATTCAAGCAATCCACGATCAATGAAGAGAATGACATGGCCTTTCTCTTGTTGATTCGGTTCTACAACTCAGGTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SLD5_C Sld5

Protein Analysis

138

Amino Acids

16.02

Weight (kDa)

9.27

Isoelectric Point (pI)

55.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sld5 PF05916 41 - 99 1.2e-06 GINS complex protein helical bundle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 16
AccBSI CCGCTC 1 cut(s) 43
AciI CCGC 1 cut(s) 41
AcsI RAATTY 1 cut(s) 165
AflII CTTAAG 1 cut(s) 47
AflIII ACRYGT 1 cut(s) 263
AgsI TTSAA 3 cut(s) 293, 310, 340
AluBI AGCT 1 cut(s) 209
AluI AGCT 1 cut(s) 209
Alw26I GTCTC 2 cut(s) 141, 236
Ama87I CYCGRG 1 cut(s) 16
AoxI GGCC 1 cut(s) 372
ApeKI GCWGC 2 cut(s) 97, 172
ApoI RAATTY 1 cut(s) 165
AspS9I GGNCC 1 cut(s) 60
AsuII TTCGAA 1 cut(s) 163
AvaI CYCGRG 1 cut(s) 16
AvaII GGWCC 1 cut(s) 60
AxyI CCTNAGG 1 cut(s) 56
BbsI GAAGAC 1 cut(s) 79
BbvI GCAGC 2 cut(s) 84, 159
BcoDI GTCTC 2 cut(s) 141, 236
BfaI CTAG 1 cut(s) 412
BfmI CTRYAG 1 cut(s) 173
BfrI CTTAAG 1 cut(s) 47
BglII AGATCT 1 cut(s) 152
BisI GCNGC 2 cut(s) 98, 173
BlsI GCNGC 2 cut(s) 99, 174
Bme18I GGWCC 1 cut(s) 60
BmeT110I CYCGRG 1 cut(s) 16
BmgT120I GGNCC 1 cut(s) 60
BmsI GCATC 1 cut(s) 295
BpiI GAAGAC 1 cut(s) 79
Bpu14I TTCGAA 1 cut(s) 163
BsaI GGTCTC 1 cut(s) 141
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bse21I CCTNAGG 1 cut(s) 56
BseGI GGATG 1 cut(s) 36
BseMII CTCAG 2 cut(s) 47, 152
BseRI GAGGAG 1 cut(s) 59
BseXI GCAGC 2 cut(s) 84, 159
BshFI GGCC 1 cut(s) 374
BsiHKCI CYCGRG 1 cut(s) 16
BsmAI GTCTC 2 cut(s) 141, 236
BsmBI CGTCTC 1 cut(s) 236
BsnI GGCC 1 cut(s) 374
Bso31I GGTCTC 1 cut(s) 141
BsoBI CYCGRG 1 cut(s) 16
Bsp119I TTCGAA 1 cut(s) 163
Bsp143I GATC 2 cut(s) 152, 351
BspACI CCGC 1 cut(s) 41
BspANI GGCC 1 cut(s) 374
BspCNI CTCAG 2 cut(s) 48, 151
BspMAI CTGCAG 1 cut(s) 177
BspT104I TTCGAA 1 cut(s) 163
BspTI CTTAAG 1 cut(s) 47
BspTNI GGTCTC 1 cut(s) 141
BsrBI CCGCTC 1 cut(s) 43
BssMI GATC 2 cut(s) 152, 351
Bst4CI ACNGT 3 cut(s) 32, 85, 230
Bst6I CTCTTC 3 cut(s) 77, 288, 354
BstAFI CTTAAG 1 cut(s) 47
BstBI TTCGAA 1 cut(s) 163
BstC8I GCNNGC 1 cut(s) 25
BstDEI CTNAG 4 cut(s) 56, 138, 205, 403
BstF5I GGATG 1 cut(s) 36
BstKTI GATC 2 cut(s) 155, 354
BstMAI GTCTC 2 cut(s) 141, 236
BstMBI GATC 2 cut(s) 152, 351
BstSFI CTRYAG 1 cut(s) 173
BstV1I GCAGC 2 cut(s) 84, 159
BstV2I GAAGAC 1 cut(s) 79
BstX2I RGATCY 1 cut(s) 152
BstYI RGATCY 1 cut(s) 152
Bsu36I CCTNAGG 1 cut(s) 56
BsuRI GGCC 1 cut(s) 374
BtsCI GGATG 1 cut(s) 36
Cac8I GCNNGC 1 cut(s) 25
Cfr13I GGNCC 1 cut(s) 60
CviAII CATG 1 cut(s) 370
CviJI RGCY 2 cut(s) 209, 374
CviKI_1 RGCY 2 cut(s) 209, 374
DdeI CTNAG 4 cut(s) 56, 138, 205, 403
DpnI GATC 2 cut(s) 154, 353
DpnII GATC 2 cut(s) 152, 351
Eam1104I CTCTTC 3 cut(s) 77, 288, 354
EarI CTCTTC 3 cut(s) 77, 288, 354
Eco31I GGTCTC 1 cut(s) 141
Eco47I GGWCC 1 cut(s) 60
Eco81I CCTNAGG 1 cut(s) 56
Eco88I CYCGRG 1 cut(s) 16
EcoO109I RGGNCCY 1 cut(s) 60
EcoRI GAATTC 1 cut(s) 165
Esp3I CGTCTC 1 cut(s) 236
FaeI CATG 1 cut(s) 373
FaiI YATR 2 cut(s) 335, 371
FatI CATG 1 cut(s) 369
FauI CCCGC 1 cut(s) 48
Fnu4HI GCNGC 2 cut(s) 98, 173
FokI GGATG 1 cut(s) 23
Fsp4HI GCNGC 2 cut(s) 98, 173
FspBI CTAG 1 cut(s) 412
GluI GCNGC 2 cut(s) 98, 173
HaeIII GGCC 1 cut(s) 374
Hin1II CATG 1 cut(s) 373
HinfI GANTC 2 cut(s) 108, 388
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 1 cut(s) 107
Hpy188III TCNNGA 1 cut(s) 290
Hpy8I GTNNAC 1 cut(s) 79
HpyAV CCTTC 1 cut(s) 241
HpyCH4III ACNGT 3 cut(s) 32, 85, 230
HpyCH4IV ACGT 1 cut(s) 263
HpyCH4V TGCA 2 cut(s) 175, 257
HpyF3I CTNAG 4 cut(s) 56, 138, 205, 403
HpySE526I ACGT 1 cut(s) 263
Hsp92II CATG 1 cut(s) 373
Kzo9I GATC 2 cut(s) 152, 351
LmnI GCTCC 2 cut(s) 4, 48
LpnPI CCDG 5 cut(s) 68, 114, 118, 227, 390
Lsp1109I GCAGC 2 cut(s) 84, 159
LweI GCATC 1 cut(s) 295
MaeI CTAG 1 cut(s) 412
MaeII ACGT 1 cut(s) 263
MaeIII GTNAC 1 cut(s) 407
MalI GATC 2 cut(s) 154, 353
MbiI CCGCTC 1 cut(s) 43
MboI GATC 2 cut(s) 152, 351
MboII GAAGA 6 cut(s) 64, 79, 152, 190, 305, 371
MflI RGATCY 1 cut(s) 152
MluCI AATT 2 cut(s) 165, 311
MmeI TCCRAC 1 cut(s) 85
MnlI CCTC 7 cut(s) 12, 25, 51, 73, 77, 80, 101
MseI TTAA 2 cut(s) 48, 318
MspCI CTTAAG 1 cut(s) 47
NdeII GATC 2 cut(s) 152, 351
NlaIII CATG 1 cut(s) 373
NmuCI GTSAC 1 cut(s) 407
NspV TTCGAA 1 cut(s) 163
PaeR7I CTCGAG 1 cut(s) 16
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PfeI GAWTC 2 cut(s) 108, 388
PkrI GCNGC 2 cut(s) 99, 174
PpuMI RGGWCCY 1 cut(s) 60
Psp5II RGGWCCY 1 cut(s) 60
PspPI GGNCC 1 cut(s) 60
PspPPI RGGWCCY 1 cut(s) 60
PspXI VCTCGAGB 1 cut(s) 16
PstI CTGCAG 1 cut(s) 177
PsuI RGATCY 1 cut(s) 152
SaqAI TTAA 2 cut(s) 48, 318
SatI GCNGC 2 cut(s) 98, 173
Sau3AI GATC 2 cut(s) 152, 351
Sau96I GGNCC 1 cut(s) 60
SetI ASST 6 cut(s) 17, 65, 137, 211, 266, 409
SfaNI GCATC 1 cut(s) 295
SfcI CTRYAG 1 cut(s) 173
Sfr274I CTCGAG 1 cut(s) 16
SfuI TTCGAA 1 cut(s) 163
SinI GGWCC 1 cut(s) 60
SlaI CTCGAG 1 cut(s) 16
SmlI CTYRAG 2 cut(s) 16, 47
SmoI CTYRAG 2 cut(s) 16, 47
Sse9I AATT 2 cut(s) 165, 311
SsiI CCGC 1 cut(s) 41
SspMI CTAG 1 cut(s) 412
TaaI ACNGT 3 cut(s) 32, 85, 230
TaiI ACGT 1 cut(s) 266
TaqI TCGA 2 cut(s) 17, 163
TasI AATT 2 cut(s) 165, 311
TfiI GAWTC 2 cut(s) 108, 388
Tru1I TTAA 2 cut(s) 48, 318
Tru9I TTAA 2 cut(s) 48, 318
TseFI GTSAC 1 cut(s) 407
TseI GCWGC 2 cut(s) 97, 172
Tsp45I GTSAC 1 cut(s) 407
TspDTI ATGAA 1 cut(s) 372
Vha464I CTTAAG 1 cut(s) 47
VpaK11BI GGWCC 1 cut(s) 60
XapI RAATTY 1 cut(s) 165
XhoI CTCGAG 1 cut(s) 16
XspI CTAG 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.