FvH4_4g20000

The GINS complex plays an essential role in the initiation of DNA replication

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006170
Physical Location & Seq
Reverse (-)
23424916 .. 23427441
2526 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g20000.t2

Sequence Viewer

Length: 465 bp
ATGGATTTGGACAGGACTCAGTTCTTGTTGAGATCTTATCTTCGAATTCGGCTGCAGAAGATTGAGAAATACATCTTTCACATCTTAGCTGCTGCTGAACTCTACAACCGTCTCTCTAAACAGAAGGCGTTTGCAAAAATGTGTCTTGTTGATTTGGAGAAGCATCTTGAAGAGAGTGTTCTGTCAAAATTGGGTAATAACTACCAGTCTATATTCCAGCAATCCATGATCAGTGAAGAGAATGACATGGTGGCAAAACTTCAATTAGACATGTTTGTTGTATGCAAAACAAATCACTATCTTGGACATATCCAGCCTGAGGACAATGAAGATGAACCATCAGATAACAGGGCGAATCAGAGACCTTTGGAGGAGCCTTTCGAAATGGAGCCTGATGTTTTATGCTTTGTGCGTTATAAAGCAGTAAAGAGATTTGTAGAGGAAGGGAGGATTGATTTATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SLD5_C Sld5

Protein Analysis

155

Amino Acids

18.29

Weight (kDa)

5.43

Isoelectric Point (pI)

62.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SLD5_C PF16922 96 - 161 4.4e-09 DNA replication complex GINS protein SLD5 C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 417
AcsI RAATTY 1 cut(s) 45
AfiI CCNNNNNNNGG 1 cut(s) 319
AflIII ACRYGT 1 cut(s) 270
AgsI TTSAA 2 cut(s) 170, 263
AluBI AGCT 1 cut(s) 89
AluI AGCT 1 cut(s) 89
Alw26I GTCTC 2 cut(s) 116, 355
ApeKI GCWGC 3 cut(s) 52, 89, 92
ApoI RAATTY 1 cut(s) 45
AsuII TTCGAA 2 cut(s) 43, 381
AxyI CCTNAGG 1 cut(s) 318
BbvI GCAGC 3 cut(s) 39, 76, 79
BccI CCATC 1 cut(s) 346
BclI TGATCA 1 cut(s) 228
BcoDI GTCTC 2 cut(s) 116, 355
BfmI CTRYAG 1 cut(s) 53
BglII AGATCT 1 cut(s) 32
BisI GCNGC 3 cut(s) 53, 90, 93
BlsI GCNGC 3 cut(s) 54, 91, 94
BmiI GGNNCC 2 cut(s) 375, 390
BmsI GCATC 1 cut(s) 172
Bpu14I TTCGAA 2 cut(s) 43, 381
BsaI GGTCTC 1 cut(s) 355
Bsc4I CCNNNNNNNGG 1 cut(s) 319
Bse1I ACTGG 1 cut(s) 205
Bse21I CCTNAGG 1 cut(s) 318
BseLI CCNNNNNNNGG 1 cut(s) 319
BseMII CTCAG 2 cut(s) 32, 309
BseNI ACTGG 1 cut(s) 205
BseRI GAGGAG 1 cut(s) 386
BseXI GCAGC 3 cut(s) 39, 76, 79
BslI CCNNNNNNNGG 1 cut(s) 319
BsmAI GTCTC 2 cut(s) 116, 355
BsmBI CGTCTC 1 cut(s) 116
Bso31I GGTCTC 1 cut(s) 355
Bsp119I TTCGAA 2 cut(s) 43, 381
Bsp143I GATC 2 cut(s) 32, 228
BspCNI CTCAG 2 cut(s) 31, 310
BspLI GGNNCC 2 cut(s) 375, 390
BspMAI CTGCAG 1 cut(s) 57
BspT104I TTCGAA 2 cut(s) 43, 381
BspTNI GGTCTC 1 cut(s) 355
BsrI ACTGG 1 cut(s) 205
BssMI GATC 2 cut(s) 32, 228
Bst4CI ACNGT 1 cut(s) 110
Bst6I CTCTTC 2 cut(s) 165, 231
BstBI TTCGAA 2 cut(s) 43, 381
BstDEI CTNAG 3 cut(s) 18, 85, 318
BstKTI GATC 2 cut(s) 35, 231
BstMAI GTCTC 2 cut(s) 116, 355
BstMBI GATC 2 cut(s) 32, 228
BstNSI RCATGY 1 cut(s) 274
BstSFI CTRYAG 1 cut(s) 53
BstV1I GCAGC 3 cut(s) 39, 76, 79
BstX2I RGATCY 1 cut(s) 32
BstYI RGATCY 1 cut(s) 32
Bsu36I CCTNAGG 1 cut(s) 318
BtsIMutI CAGTG 1 cut(s) 238
CviAII CATG 3 cut(s) 226, 247, 271
CviJI RGCY 5 cut(s) 52, 89, 316, 376, 391
CviKI_1 RGCY 5 cut(s) 52, 89, 316, 376, 391
DdeI CTNAG 3 cut(s) 18, 85, 318
DpnI GATC 2 cut(s) 34, 230
DpnII GATC 2 cut(s) 32, 228
Eam1104I CTCTTC 2 cut(s) 165, 231
EarI CTCTTC 2 cut(s) 165, 231
Eco31I GGTCTC 1 cut(s) 355
Eco81I CCTNAGG 1 cut(s) 318
EcoRI GAATTC 1 cut(s) 45
Esp3I CGTCTC 1 cut(s) 116
FaeI CATG 3 cut(s) 229, 250, 274
FaiI YATR 8 cut(s) 212, 227, 248, 272, 283, 309, 403, 417
FatI CATG 3 cut(s) 225, 246, 270
FbaI TGATCA 1 cut(s) 228
Fnu4HI GCNGC 3 cut(s) 53, 90, 93
Fsp4HI GCNGC 3 cut(s) 53, 90, 93
GluI GCNGC 3 cut(s) 53, 90, 93
Hin1II CATG 3 cut(s) 229, 250, 274
HinfI GANTC 2 cut(s) 16, 355
Hpy188I TCNGA 3 cut(s) 343, 360, 464
Hpy188III TCNNGA 1 cut(s) 167
HpyAV CCTTC 2 cut(s) 118, 437
HpyCH4III ACNGT 1 cut(s) 110
HpyCH4V TGCA 3 cut(s) 55, 134, 285
HpyF3I CTNAG 3 cut(s) 18, 85, 318
Hsp92II CATG 3 cut(s) 229, 250, 274
Ksp22I TGATCA 1 cut(s) 228
Kzo9I GATC 2 cut(s) 32, 228
LmnI GCTCC 2 cut(s) 373, 388
LpnPI CCDG 6 cut(s) 218, 230, 326, 330, 334, 405
Lsp1109I GCAGC 3 cut(s) 39, 76, 79
LweI GCATC 1 cut(s) 172
MalI GATC 2 cut(s) 34, 230
MboI GATC 2 cut(s) 32, 228
MboII GAAGA 5 cut(s) 32, 70, 182, 248, 341
MflI RGATCY 1 cut(s) 32
MluCI AATT 3 cut(s) 45, 188, 263
MlyI GAGTC 1 cut(s) 10
MnlI CCTC 4 cut(s) 313, 364, 433, 441
NdeII GATC 2 cut(s) 32, 228
NlaIII CATG 3 cut(s) 229, 250, 274
NlaIV GGNNCC 2 cut(s) 375, 390
NspI RCATGY 1 cut(s) 274
NspV TTCGAA 2 cut(s) 43, 381
PciI ACATGT 1 cut(s) 270
PfeI GAWTC 1 cut(s) 355
PkrI GCNGC 3 cut(s) 54, 91, 94
PleI GAGTC 1 cut(s) 10
PpsI GAGTC 1 cut(s) 10
PscI ACATGT 1 cut(s) 270
PsiI TTATAA 1 cut(s) 417
PspN4I GGNNCC 2 cut(s) 375, 390
PstI CTGCAG 1 cut(s) 57
PsuI RGATCY 1 cut(s) 32
SatI GCNGC 3 cut(s) 53, 90, 93
Sau3AI GATC 2 cut(s) 32, 228
SchI GAGTC 1 cut(s) 10
SetI ASST 2 cut(s) 91, 367
SfaNI GCATC 1 cut(s) 172
SfcI CTRYAG 1 cut(s) 53
SfuI TTCGAA 2 cut(s) 43, 381
Sse9I AATT 3 cut(s) 45, 188, 263
TaaI ACNGT 1 cut(s) 110
TaqI TCGA 2 cut(s) 43, 381
TasI AATT 3 cut(s) 45, 188, 263
TfiI GAWTC 1 cut(s) 355
TscAI CASTG 1 cut(s) 238
TseI GCWGC 3 cut(s) 52, 89, 92
TspDTI ATGAA 2 cut(s) 342, 348
TspRI CASTG 1 cut(s) 238
XapI RAATTY 1 cut(s) 45
XceI RCATGY 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.