Rorug04G0205400

The GINS complex plays an essential role in the initiation of DNA replication

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0006170
Physical Location & Seq
Reverse (-)
35802483 .. 35805486
3004 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0205400.1

Sequence Viewer

Length: 789 bp
ATGGGAAGGCGAGAAGGCAGTGAAACGGAAAACCCAAAACAAAATTCCAAGAACTGTTTTCAGAGCCTGCAATTAATTTTAAACAAAGACACCTTAATAATGGATTGTATTTTGAAGATAATGAATCAGCTTCTTAGCATGAGCATAGGAGGATCAAGTTACTTTCAACAAGTGAATGAGAGTGAGGCTGAAAGAGCGAGAAAATTAGCAAGGGTGGTAGATTTGGACCTTAACCACCCACCTCCTAGTGAGAACCTGGCTGTAGCTGCTCCTTCGAGCGTCCAACCTCCACAACAATCTTCGGACGCTCCTGATGATGAATGTCTAATCATATCACCACGGAAGTTTGAAGAGGCCATGAATAATTCTCAGAGAAATCCGTCTCGCAGAGTAAGAAGCAATATAGAAGATTTTACTGAAGTTGGTGTTGCACAATCGTTTGATATCTTATCAACAGACTTGATAACATGGGGGATCTCCTTTGGCCGACTAGTTGGAACTGTCAACGGAGTGATTTCCTTGAATGATGAAGTTCTTAACAAAACCAAAGATACTGAAACCAGTATCCCCATGGCTCCAATAGAGCCCCAGGGTGAGCCCGAGGCACCTGCGTTTTCTTGTGTAATCTGCATGGGCCAATTGGTTGAAGAAACATCAACGAAGTGCGGCCACATCTTCTGCAAGATGTGTATTGAAAAAGCAATATCTACTCAACACAAATGCCCTAACTGCAGGCGTAAACTTAGAAAGCGAGACATCTTCAGGGTCCACCTTCCATACACCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SLD5_C Sld5

Protein Analysis

262

Amino Acids

29.21

Weight (kDa)

5.89

Isoelectric Point (pI)

70.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 206 - 245 6.1e-09 Ring finger domain
zf-C3HC4_3 PF13920 206 - 250 2.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 207 - 244 4.1e-09 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 207 - 244 2.8e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 616
Acc36I ACCTGC 1 cut(s) 616
AccB1I GGYRCC 1 cut(s) 604
AciI CCGC 1 cut(s) 666
AclWI GGATC 2 cut(s) 160, 482
AcoI YGGCCR 2 cut(s) 484, 667
AcsI RAATTY 1 cut(s) 43
AcuI CTGAAG 2 cut(s) 438, 745
AgsI TTSAA 6 cut(s) 115, 167, 350, 523, 647, 695
AhlI ACTAGT 1 cut(s) 490
AjnI CCWGG 2 cut(s) 255, 588
AjuI GAANNNNNNNTTGG 2 cut(s) 28, 60
AluBI AGCT 2 cut(s) 130, 266
AluI AGCT 2 cut(s) 130, 266
Alw26I GTCTC 2 cut(s) 387, 747
AlwI GGATC 2 cut(s) 160, 482
AlwNI CAGNNNCTG 1 cut(s) 67
Ama87I CYCGRG 1 cut(s) 599
AoxI GGCC 4 cut(s) 354, 484, 634, 667
ApeKI GCWGC 1 cut(s) 266
ApoI RAATTY 1 cut(s) 43
AseI ATTAAT 1 cut(s) 74
AspS9I GGNCC 3 cut(s) 226, 634, 766
AsuHPI GGTGA 2 cut(s) 327, 605
AvaI CYCGRG 1 cut(s) 599
AvaII GGWCC 2 cut(s) 226, 766
BanI GGYRCC 1 cut(s) 604
BanII GRGCYC 2 cut(s) 588, 600
BbvI GCAGC 1 cut(s) 253
BcgI CGANNNNNNTGC 2 cut(s) 590, 624
BciT130I CCWGG 2 cut(s) 257, 590
BciVI GTATCC 1 cut(s) 575
BcoDI GTCTC 2 cut(s) 387, 747
BcuI ACTAGT 1 cut(s) 490
BfaI CTAG 2 cut(s) 246, 491
BfmI CTRYAG 2 cut(s) 261, 730
BfuAI ACCTGC 1 cut(s) 616
BfuI GTATCC 1 cut(s) 575
BisI GCNGC 2 cut(s) 267, 667
BlsI GCNGC 2 cut(s) 268, 668
Bme1390I CCNGG 2 cut(s) 257, 590
Bme18I GGWCC 2 cut(s) 226, 766
BmeT110I CYCGRG 1 cut(s) 599
BmgT120I GGNCC 3 cut(s) 226, 634, 766
BmiI GGNNCC 3 cut(s) 576, 606, 767
BmrFI CCNGG 2 cut(s) 257, 590
BsaJI CCNNGG 5 cut(s) 338, 570, 588, 589, 600
Bse1I ACTGG 2 cut(s) 561, 783
BseBI CCWGG 2 cut(s) 257, 590
BseDI CCNNGG 5 cut(s) 338, 570, 588, 589, 600
BseMII CTCAG 1 cut(s) 383
BseNI ACTGG 2 cut(s) 561, 783
BseXI GCAGC 1 cut(s) 253
BshFI GGCC 4 cut(s) 356, 486, 636, 669
BshNI GGYRCC 1 cut(s) 604
BsiHKCI CYCGRG 1 cut(s) 599
BsmAI GTCTC 2 cut(s) 387, 747
BsmBI CGTCTC 1 cut(s) 387
BsnI GGCC 4 cut(s) 356, 486, 636, 669
BsoBI CYCGRG 1 cut(s) 599
Bsp1286I GDGCHC 2 cut(s) 588, 600
Bsp143I GATC 2 cut(s) 152, 474
Bsp19I CCATGG 1 cut(s) 570
BspACI CCGC 1 cut(s) 666
BspANI GGCC 4 cut(s) 356, 486, 636, 669
BspCNI CTCAG 1 cut(s) 382
BspLI GGNNCC 3 cut(s) 576, 606, 767
BspMAI CTGCAG 1 cut(s) 734
BspMI ACCTGC 1 cut(s) 616
BspPI GGATC 2 cut(s) 160, 482
BspT107I GGYRCC 1 cut(s) 604
BsrI ACTGG 2 cut(s) 561, 783
BssECI CCNNGG 5 cut(s) 338, 570, 588, 589, 600
BssMI GATC 2 cut(s) 152, 474
BssT1I CCWWGG 1 cut(s) 570
Bst2UI CCWGG 2 cut(s) 257, 590
Bst4CI ACNGT 2 cut(s) 56, 502
Bst6I CTCTTC 1 cut(s) 345
BstC8I GCNNGC 2 cut(s) 68, 734
BstDEI CTNAG 3 cut(s) 134, 369, 743
BstDSI CCRYGG 2 cut(s) 338, 570
BstKTI GATC 2 cut(s) 155, 477
BstMAI GTCTC 2 cut(s) 387, 747
BstMBI GATC 2 cut(s) 152, 474
BstMWI GCNNNNNNNGC 3 cut(s) 194, 266, 729
BstNI CCWGG 2 cut(s) 257, 590
BstSCI CCNGG 2 cut(s) 255, 588
BstSFI CTRYAG 2 cut(s) 261, 730
BstV1I GCAGC 1 cut(s) 253
BstX2I RGATCY 1 cut(s) 474
BstYI RGATCY 1 cut(s) 474
BsuI GTATCC 1 cut(s) 575
BsuRI GGCC 4 cut(s) 356, 486, 636, 669
BtgI CCRYGG 2 cut(s) 338, 570
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 1 cut(s) 25
BveI ACCTGC 1 cut(s) 616
Cac8I GCNNGC 2 cut(s) 68, 734
CaiI CAGNNNCTG 1 cut(s) 67
Cfr13I GGNCC 3 cut(s) 226, 634, 766
CseI GACGC 2 cut(s) 268, 314
CviAII CATG 5 cut(s) 139, 358, 468, 571, 631
DdeI CTNAG 3 cut(s) 134, 369, 743
DpnI GATC 2 cut(s) 154, 476
DpnII GATC 2 cut(s) 152, 474
DraI TTTAAA 1 cut(s) 81
EaeI YGGCCR 2 cut(s) 484, 667
Eam1104I CTCTTC 1 cut(s) 345
EarI CTCTTC 1 cut(s) 345
Eco130I CCWWGG 1 cut(s) 570
Eco24I GRGCYC 2 cut(s) 588, 600
Eco32I GATATC 1 cut(s) 445
Eco47I GGWCC 2 cut(s) 226, 766
Eco57I CTGAAG 2 cut(s) 438, 745
Eco88I CYCGRG 1 cut(s) 599
EcoRII CCWGG 2 cut(s) 255, 588
EcoRV GATATC 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 570
EcoT38I GRGCYC 2 cut(s) 588, 600
ErhI CCWWGG 1 cut(s) 570
Esp3I CGTCTC 1 cut(s) 387
FaeI CATG 5 cut(s) 142, 361, 471, 574, 634
FaiI YATR 9 cut(s) 140, 146, 332, 359, 404, 469, 572, 632, 778
FatI CATG 5 cut(s) 138, 357, 467, 570, 630
Fnu4HI GCNGC 2 cut(s) 267, 667
FriOI GRGCYC 2 cut(s) 588, 600
Fsp4HI GCNGC 2 cut(s) 267, 667
FspBI CTAG 2 cut(s) 246, 491
GluI GCNGC 2 cut(s) 267, 667
HaeIII GGCC 4 cut(s) 356, 486, 636, 669
HgaI GACGC 2 cut(s) 268, 314
Hin1II CATG 5 cut(s) 142, 361, 471, 574, 634
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HinfI GANTC 1 cut(s) 124
HphI GGTGA 2 cut(s) 327, 605
Hpy166II GTNNAC 3 cut(s) 505, 740, 769
Hpy188I TCNGA 3 cut(s) 63, 304, 372
Hpy188III TCNNGA 1 cut(s) 311
Hpy8I GTNNAC 3 cut(s) 505, 740, 769
HpyAV CCTTC 3 cut(s) 8, 282, 782
HpyCH4III ACNGT 2 cut(s) 56, 502
HpyCH4V TGCA 5 cut(s) 70, 431, 630, 681, 732
HpyF10VI GCNNNNNNNGC 3 cut(s) 194, 266, 729
HpyF3I CTNAG 3 cut(s) 134, 369, 743
Hsp92II CATG 5 cut(s) 142, 361, 471, 574, 634
Kzo9I GATC 2 cut(s) 152, 474
LmnI GCTCC 3 cut(s) 274, 313, 580
Lsp1109I GCAGC 1 cut(s) 253
MaeI CTAG 2 cut(s) 246, 491
MaeIII GTNAC 1 cut(s) 158
MalI GATC 2 cut(s) 154, 476
MboI GATC 2 cut(s) 152, 474
MboII GAAGA 7 cut(s) 127, 291, 362, 419, 659, 667, 751
MfeI CAATTG 1 cut(s) 638
MflI RGATCY 1 cut(s) 474
MhlI GDGCHC 2 cut(s) 588, 600
MluCI AATT 6 cut(s) 43, 71, 75, 203, 364, 638
MmeI TCCRAC 2 cut(s) 307, 475
MnlI CCTC 6 cut(s) 143, 178, 252, 297, 346, 595
MseI TTAA 6 cut(s) 74, 80, 95, 231, 537, 787
MspR9I CCNGG 2 cut(s) 257, 590
MunI CAATTG 1 cut(s) 638
MvaI CCWGG 2 cut(s) 257, 590
MwoI GCNNNNNNNGC 3 cut(s) 194, 266, 729
NcoI CCATGG 1 cut(s) 570
NdeII GATC 2 cut(s) 152, 474
NlaIII CATG 5 cut(s) 142, 361, 471, 574, 634
NlaIV GGNNCC 3 cut(s) 576, 606, 767
PaqCI CACCTGC 1 cut(s) 616
PasI CCCWGGG 1 cut(s) 589
PfeI GAWTC 1 cut(s) 124
PkrI GCNGC 2 cut(s) 268, 668
PshBI ATTAAT 1 cut(s) 74
Psp6I CCWGG 2 cut(s) 255, 588
PspGI CCWGG 2 cut(s) 255, 588
PspN4I GGNNCC 3 cut(s) 576, 606, 767
PspPI GGNCC 3 cut(s) 226, 634, 766
PstI CTGCAG 1 cut(s) 734
PstNI CAGNNNCTG 1 cut(s) 67
PsuI RGATCY 1 cut(s) 474
SaqAI TTAA 6 cut(s) 74, 80, 95, 231, 537, 787
SatI GCNGC 2 cut(s) 267, 667
Sau3AI GATC 2 cut(s) 152, 474
Sau96I GGNCC 3 cut(s) 226, 634, 766
ScrFI CCNGG 2 cut(s) 257, 590
SduI GDGCHC 2 cut(s) 588, 600
SetI ASST 9 cut(s) 95, 132, 231, 244, 258, 268, 289, 610, 774
SfcI CTRYAG 2 cut(s) 261, 730
SinI GGWCC 2 cut(s) 226, 766
SpeI ACTAGT 1 cut(s) 490
Sse9I AATT 6 cut(s) 43, 71, 75, 203, 364, 638
SsiI CCGC 1 cut(s) 666
SspMI CTAG 2 cut(s) 246, 491
StyD4I CCNGG 2 cut(s) 255, 588
StyI CCWWGG 1 cut(s) 570
TaaI ACNGT 2 cut(s) 56, 502
TaqI TCGA 1 cut(s) 275
TasI AATT 6 cut(s) 43, 71, 75, 203, 364, 638
TauI GCSGC 1 cut(s) 669
TfiI GAWTC 1 cut(s) 124
Tru1I TTAA 6 cut(s) 74, 80, 95, 231, 537, 787
Tru9I TTAA 6 cut(s) 74, 80, 95, 231, 537, 787
TscAI CASTG 1 cut(s) 25
TseI GCWGC 1 cut(s) 266
TspDTI ATGAA 4 cut(s) 137, 333, 374, 543
TspGWI ACGGA 4 cut(s) 41, 355, 369, 522
TspRI CASTG 1 cut(s) 25
VpaK11BI GGWCC 2 cut(s) 226, 766
VspI ATTAAT 1 cut(s) 74
XapI RAATTY 1 cut(s) 43
XcmI CCANNNNNNNNNTGG 1 cut(s) 568
XspI CTAG 2 cut(s) 246, 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.