Rh4CG279800

The GINS complex plays an essential role in the initiation of DNA replication

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0006170
Physical Location & Seq
Reverse (-)
53643784 .. 53646759
2976 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG279800.1

Sequence Viewer

Length: 558 bp
ATGGACGCGAGAGACGGAGACGGGTCAACGGAGGAATACGAGACGTTGATTCAGACGACCGACGTCGAGCTTCTGAAGAGGGCGTGGCGGCAAGAGAAGGCGGCTCCGGAGATTTTGAAATTCGAGACTGATTTGATTGCCAGGCTCACTGGACAGATCGAATTGGTGGAAGAAAATGTGGAGCAAGATGCTCAATCTGGTATTGATCCACTTACTGTATCACTCTACCAGATGGATTTGGACAGGTCTCAGTTCTTGTTGAGATCTTATCTTCGAATTCGGCTGCAGAAGATTGAGAAATACATCTTTCACATCTTAGCTACTGCTGAACTCTACAACCGTCTCTCTAAACAGGAGAAGGCATTTGCAAAAACGTGTCTTGTTGATTTGGAGAAGCATCTTGAAGAGAGTGTTCTGTCGAAATTGCCTAATAACTATCAGTCTATATTCAAGCAATCCATGATCAGTGAAGAGAATGACATGGGTACTCATATTATCTTCTTTCCAGATTTCATATATCTCTGTGTTTTCCGTTTTGCATGTGTGTGTGTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SLD5_C Sld5

Protein Analysis

185

Amino Acids

21.6

Weight (kDa)

4.84

Isoelectric Point (pI)

58.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sld5 PF05916 71 - 136 1.1e-11 GINS complex protein helical bundle domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 66
AccII CGCG 1 cut(s) 8
AccIII TCCGGA 1 cut(s) 106
AciI CCGC 2 cut(s) 88, 101
AclWI GGATC 1 cut(s) 200
AcsI RAATTY 2 cut(s) 119, 276
AcuI CTGAAG 1 cut(s) 95
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 1 cut(s) 487
AflIII ACRYGT 1 cut(s) 374
AgsI TTSAA 3 cut(s) 118, 404, 451
AjnI CCWGG 1 cut(s) 140
AluBI AGCT 2 cut(s) 70, 320
AluI AGCT 2 cut(s) 70, 320
Alw26I GTCTC 6 cut(s) 6, 12, 35, 119, 252, 347
AlwI GGATC 1 cut(s) 200
Aor13HI TCCGGA 1 cut(s) 106
ApeKI GCWGC 1 cut(s) 283
ApoI RAATTY 2 cut(s) 119, 276
AsuII TTCGAA 1 cut(s) 274
BbvI GCAGC 1 cut(s) 270
BccI CCATC 1 cut(s) 226
BciT130I CCWGG 1 cut(s) 142
BclI TGATCA 1 cut(s) 462
BcoDI GTCTC 6 cut(s) 6, 12, 35, 119, 252, 347
BfmI CTRYAG 1 cut(s) 284
BglII AGATCT 1 cut(s) 263
BisI GCNGC 3 cut(s) 89, 102, 284
BlsI GCNGC 3 cut(s) 90, 103, 285
Bme1390I CCNGG 1 cut(s) 142
BmiI GGNNCC 1 cut(s) 105
BmrFI CCNGG 1 cut(s) 142
BmsI GCATC 2 cut(s) 178, 406
BoxI GACNNNNGTC 1 cut(s) 62
Bpu14I TTCGAA 1 cut(s) 274
BsaHI GRCGYC 1 cut(s) 63
BsaI GGTCTC 1 cut(s) 252
BsaWI WCCGGW 1 cut(s) 106
Bse1I ACTGG 1 cut(s) 154
BseAI TCCGGA 1 cut(s) 106
BseBI CCWGG 1 cut(s) 142
BseMII CTCAG 1 cut(s) 263
BseNI ACTGG 1 cut(s) 154
BseXI GCAGC 1 cut(s) 270
Bsh1236I CGCG 1 cut(s) 8
Bsh1285I CGRYCG 1 cut(s) 60
BsiEI CGRYCG 1 cut(s) 60
BsiSI CCGG 1 cut(s) 107
BsmAI GTCTC 6 cut(s) 6, 12, 35, 119, 252, 347
BsmBI CGTCTC 4 cut(s) 6, 12, 35, 347
Bso31I GGTCTC 1 cut(s) 252
Bsp119I TTCGAA 1 cut(s) 274
Bsp13I TCCGGA 1 cut(s) 106
Bsp143I GATC 4 cut(s) 156, 205, 263, 462
BspACI CCGC 2 cut(s) 88, 101
BspCNI CTCAG 1 cut(s) 262
BspEI TCCGGA 1 cut(s) 106
BspFNI CGCG 1 cut(s) 8
BspLI GGNNCC 1 cut(s) 105
BspMAI CTGCAG 1 cut(s) 288
BspPI GGATC 1 cut(s) 200
BspT104I TTCGAA 1 cut(s) 274
BspTNI GGTCTC 1 cut(s) 252
BsrI ACTGG 1 cut(s) 154
BssMI GATC 4 cut(s) 156, 205, 263, 462
BssNI GRCGYC 1 cut(s) 63
Bst2UI CCWGG 1 cut(s) 142
Bst4CI ACNGT 2 cut(s) 217, 341
Bst6I CTCTTC 3 cut(s) 71, 399, 465
BstACI GRCGYC 1 cut(s) 63
BstBI TTCGAA 1 cut(s) 274
BstDEI CTNAG 2 cut(s) 249, 316
BstFNI CGCG 1 cut(s) 8
BstKTI GATC 4 cut(s) 159, 208, 266, 465
BstMAI GTCTC 6 cut(s) 6, 12, 35, 119, 252, 347
BstMBI GATC 4 cut(s) 156, 205, 263, 462
BstMCI CGRYCG 1 cut(s) 60
BstNI CCWGG 1 cut(s) 142
BstNSI RCATGY 1 cut(s) 543
BstPAI GACNNNNGTC 1 cut(s) 62
BstSCI CCNGG 1 cut(s) 140
BstSFI CTRYAG 1 cut(s) 284
BstUI CGCG 1 cut(s) 8
BstV1I GCAGC 1 cut(s) 270
BstX2I RGATCY 1 cut(s) 263
BstYI RGATCY 1 cut(s) 263
BtsIMutI CAGTG 2 cut(s) 147, 472
CseI GACGC 1 cut(s) 14
Csp6I GTAC 1 cut(s) 486
CviAII CATG 3 cut(s) 460, 481, 540
CviJI RGCY 5 cut(s) 70, 104, 145, 283, 320
CviKI_1 RGCY 5 cut(s) 70, 104, 145, 283, 320
CviQI GTAC 1 cut(s) 486
DdeI CTNAG 2 cut(s) 249, 316
DpnI GATC 4 cut(s) 158, 207, 265, 464
DpnII GATC 4 cut(s) 156, 205, 263, 462
Eam1104I CTCTTC 3 cut(s) 71, 399, 465
EarI CTCTTC 3 cut(s) 71, 399, 465
Eco31I GGTCTC 1 cut(s) 252
Eco57I CTGAAG 1 cut(s) 95
EcoRI GAATTC 1 cut(s) 276
EcoRII CCWGG 1 cut(s) 140
Esp3I CGTCTC 4 cut(s) 6, 12, 35, 347
FaeI CATG 3 cut(s) 463, 484, 543
FaiI YATR 7 cut(s) 446, 461, 482, 492, 515, 517, 541
FatI CATG 3 cut(s) 459, 480, 539
FbaI TGATCA 1 cut(s) 462
Fnu4HI GCNGC 3 cut(s) 89, 102, 284
Fsp4HI GCNGC 3 cut(s) 89, 102, 284
GluI GCNGC 3 cut(s) 89, 102, 284
HapII CCGG 1 cut(s) 107
HgaI GACGC 1 cut(s) 14
Hin1I GRCGYC 1 cut(s) 63
Hin1II CATG 3 cut(s) 463, 484, 543
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HinfI GANTC 1 cut(s) 49
HpaII CCGG 1 cut(s) 107
Hpy166II GTNNAC 1 cut(s) 27
Hpy188I TCNGA 2 cut(s) 54, 75
Hpy188III TCNNGA 4 cut(s) 107, 124, 401, 506
Hpy8I GTNNAC 1 cut(s) 27
Hpy99I CGWCG 2 cut(s) 65, 68
HpyAV CCTTC 2 cut(s) 91, 352
HpyCH4III ACNGT 2 cut(s) 217, 341
HpyCH4IV ACGT 3 cut(s) 44, 63, 374
HpyCH4V TGCA 3 cut(s) 286, 368, 539
HpyF3I CTNAG 2 cut(s) 249, 316
HpySE526I ACGT 3 cut(s) 44, 63, 374
Hsp92I GRCGYC 1 cut(s) 63
Hsp92II CATG 3 cut(s) 463, 484, 543
Kpn2I TCCGGA 1 cut(s) 106
Ksp22I TGATCA 1 cut(s) 462
Kzo9I GATC 4 cut(s) 156, 205, 263, 462
LmnI GCTCC 2 cut(s) 109, 181
LpnPI CCDG 9 cut(s) 120, 127, 135, 154, 183, 229, 242, 338, 519
Lsp1109I GCAGC 1 cut(s) 270
LweI GCATC 2 cut(s) 178, 406
MaeII ACGT 3 cut(s) 44, 63, 374
MalI GATC 4 cut(s) 158, 207, 265, 464
MboI GATC 4 cut(s) 156, 205, 263, 462
MboII GAAGA 7 cut(s) 88, 182, 263, 301, 416, 482, 490
MflI RGATCY 1 cut(s) 263
MluCI AATT 4 cut(s) 119, 161, 276, 422
MnlI CCTC 2 cut(s) 25, 72
MroI TCCGGA 1 cut(s) 106
MslI CAYNNNNRTG 1 cut(s) 544
MspI CCGG 1 cut(s) 107
MspR9I CCNGG 1 cut(s) 142
MvaI CCWGG 1 cut(s) 142
MvnI CGCG 1 cut(s) 8
NdeII GATC 4 cut(s) 156, 205, 263, 462
NlaIII CATG 3 cut(s) 463, 484, 543
NlaIV GGNNCC 1 cut(s) 105
NspI RCATGY 1 cut(s) 543
NspV TTCGAA 1 cut(s) 274
PfeI GAWTC 1 cut(s) 49
PkrI GCNGC 3 cut(s) 90, 103, 285
PshAI GACNNNNGTC 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 140
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 1 cut(s) 105
PstI CTGCAG 1 cut(s) 288
PsuI RGATCY 1 cut(s) 263
RsaI GTAC 1 cut(s) 487
RsaNI GTAC 1 cut(s) 486
RseI CAYNNNNRTG 1 cut(s) 544
SatI GCNGC 3 cut(s) 89, 102, 284
Sau3AI GATC 4 cut(s) 156, 205, 263, 462
ScrFI CCNGG 1 cut(s) 142
SetI ASST 6 cut(s) 47, 66, 72, 248, 322, 377
SfaNI GCATC 2 cut(s) 178, 406
SfcI CTRYAG 1 cut(s) 284
SfuI TTCGAA 1 cut(s) 274
SmiMI CAYNNNNRTG 1 cut(s) 544
Sse9I AATT 4 cut(s) 119, 161, 276, 422
SsiI CCGC 2 cut(s) 88, 101
StyD4I CCNGG 1 cut(s) 140
TaaI ACNGT 2 cut(s) 217, 341
TaiI ACGT 3 cut(s) 47, 66, 377
TaqI TCGA 5 cut(s) 66, 123, 159, 274, 419
TaqII GACCGA 1 cut(s) 74
TasI AATT 4 cut(s) 119, 161, 276, 422
TauI GCSGC 2 cut(s) 91, 104
TfiI GAWTC 1 cut(s) 49
TscAI CASTG 2 cut(s) 154, 472
TseI GCWGC 1 cut(s) 283
TspDTI ATGAA 1 cut(s) 502
TspGWI ACGGA 3 cut(s) 30, 44, 521
TspRI CASTG 2 cut(s) 154, 472
XapI RAATTY 2 cut(s) 119, 276
XceI RCATGY 1 cut(s) 543
ZraI GACGTC 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.