FvH4_4g16440

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010261
Physical Location & Seq
Forward (+)
20390573 .. 20394870
4298 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g16440.t1

Sequence Viewer

Length: 543 bp
ATGGGATTTGGAGCACTAAGGTCCTTGTGTCAACCCCTATCACGAACCCTAATTTCTCGCACCTCGACTTGCTCCATAACATCATTTGCTGCCAACTCTGTGCACCTGAAACCTGAGCGCCGCTTTGTCCCCAGTGGTCAGGCTCCATGGTTTCTTCTGATCCTGAACCATTTTCACAGCTTGACAGACACTCGTTTCCAGAAACGGCGTCCCAGTGATAAGCCTCGTCGAAAAAGATCCAGCATAAGACCTCCTGGACCATATGCTGGGGTTCAGGATGTTCCTGGTGAACCAGTAGTGCCTAGCAGACCCAATGAAGGTAGTGTGAAGAGGAGGAATGAGAAGAAGCGCATGAGGCAGCGCCATGCATTTATTTTGTCAGAGAAAAAGAAGAGGAAAGCTCTGGTACAAGAAGCCAAAAGAAAGAAAAACATTAAGAGAATTGAGAATAAAATGGCGGCAGTTGCAAGAGACAGAGCGTGGGCTCAGAGGCTGGCTGAGCTGCAGCAGTTGGAGGAAGAGAAGAAGAAACCCATGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

20.77

Weight (kDa)

11.49

Isoelectric Point (pI)

78.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 266
AciI CCGC 2 cut(s) 121, 458
AclWI GGATC 2 cut(s) 154, 231
AcyI GRCGYC 1 cut(s) 208
AfaI GTAC 1 cut(s) 408
AfiI CCNNNNNNNGG 2 cut(s) 266, 317
AjnI CCWGG 2 cut(s) 253, 283
AluBI AGCT 3 cut(s) 180, 401, 502
AluI AGCT 3 cut(s) 180, 401, 502
Alw21I GWGCWC 2 cut(s) 16, 105
Alw26I GTCTC 1 cut(s) 465
Alw44I GTGCAC 1 cut(s) 101
AlwI GGATC 2 cut(s) 154, 231
AlwNI CAGNNNCTG 1 cut(s) 493
ApaLI GTGCAC 1 cut(s) 101
ApeKI GCWGC 4 cut(s) 89, 358, 502, 505
AspLEI GCGC 3 cut(s) 120, 351, 363
AspS9I GGNCC 2 cut(s) 21, 257
AsuHPI GGTGA 1 cut(s) 299
AvaII GGWCC 2 cut(s) 21, 257
BaeGI GKGCMC 1 cut(s) 105
BanII GRGCYC 1 cut(s) 487
Bbv12I GWGCWC 2 cut(s) 16, 105
BbvI GCAGC 4 cut(s) 76, 370, 489, 517
BceAI ACGGC 1 cut(s) 221
BciT130I CCWGG 2 cut(s) 255, 285
BcoDI GTCTC 1 cut(s) 465
BfaI CTAG 1 cut(s) 303
BfmI CTRYAG 1 cut(s) 503
BfoI RGCGCY 2 cut(s) 121, 364
BisI GCNGC 6 cut(s) 90, 121, 359, 459, 503, 506
BlpI GCTNAGC 1 cut(s) 498
BlsI GCNGC 6 cut(s) 91, 122, 360, 460, 504, 507
Bme1390I CCNGG 2 cut(s) 255, 285
Bme18I GGWCC 2 cut(s) 21, 257
BmgT120I GGNCC 2 cut(s) 21, 257
BmiI GGNNCC 1 cut(s) 144
BmrFI CCNGG 2 cut(s) 255, 285
BmrI ACTGGG 2 cut(s) 126, 207
BmuI ACTGGG 2 cut(s) 126, 207
BplI GAGNNNNNCTC 2 cut(s) 385, 417
Bpu10I CCTNAGC 1 cut(s) 114
Bpu1102I GCTNAGC 1 cut(s) 498
BsaHI GRCGYC 1 cut(s) 208
BsaJI CCNNGG 2 cut(s) 146, 534
Bsc4I CCNNNNNNNGG 2 cut(s) 266, 317
Bse1I ACTGG 3 cut(s) 132, 213, 293
BseBI CCWGG 2 cut(s) 255, 285
BseDI CCNNGG 2 cut(s) 146, 534
BseGI GGATG 1 cut(s) 283
BseLI CCNNNNNNNGG 2 cut(s) 266, 317
BseMII CTCAG 3 cut(s) 105, 489, 500
BseNI ACTGG 3 cut(s) 132, 213, 293
BseRI GAGGAG 1 cut(s) 346
BseSI GKGCMC 1 cut(s) 105
BseXI GCAGC 4 cut(s) 76, 370, 489, 517
BseYI CCCAGC 1 cut(s) 266
BsiHKAI GWGCWC 2 cut(s) 16, 105
BslFI GGGAC 2 cut(s) 113, 195
BslI CCNNNNNNNGG 2 cut(s) 266, 317
BsmAI GTCTC 1 cut(s) 465
BsmFI GGGAC 2 cut(s) 113, 195
Bsp1286I GDGCHC 3 cut(s) 16, 105, 487
Bsp143I GATC 2 cut(s) 159, 236
Bsp1720I GCTNAGC 1 cut(s) 498
Bsp19I CCATGG 2 cut(s) 146, 534
BspACI CCGC 2 cut(s) 121, 458
BspCNI CTCAG 3 cut(s) 106, 490, 499
BspLI GGNNCC 1 cut(s) 144
BspMAI CTGCAG 1 cut(s) 507
BspPI GGATC 2 cut(s) 154, 231
BsrI ACTGG 3 cut(s) 132, 213, 293
BssECI CCNNGG 2 cut(s) 146, 534
BssMI GATC 2 cut(s) 159, 236
BssNI GRCGYC 1 cut(s) 208
BssT1I CCWWGG 2 cut(s) 146, 534
Bst2UI CCWGG 2 cut(s) 255, 285
Bst6I CTCTTC 3 cut(s) 323, 386, 513
BstACI GRCGYC 1 cut(s) 208
BstC8I GCNNGC 1 cut(s) 495
BstDEI CTNAG 4 cut(s) 17, 114, 486, 498
BstDSI CCRYGG 2 cut(s) 146, 534
BstF5I GGATG 1 cut(s) 283
BstH2I RGCGCY 2 cut(s) 121, 364
BstHHI GCGC 3 cut(s) 120, 351, 363
BstKTI GATC 2 cut(s) 162, 239
BstMAI GTCTC 1 cut(s) 465
BstMBI GATC 2 cut(s) 159, 236
BstMWI GCNNNNNNNGC 3 cut(s) 355, 464, 499
BstNI CCWGG 2 cut(s) 255, 285
BstSCI CCNGG 2 cut(s) 253, 283
BstSFI CTRYAG 1 cut(s) 503
BstSLI GKGCMC 1 cut(s) 105
BstV1I GCAGC 4 cut(s) 76, 370, 489, 517
BstX2I RGATCY 1 cut(s) 236
BstYI RGATCY 1 cut(s) 236
BtgI CCRYGG 2 cut(s) 146, 534
BtsCI GGATG 1 cut(s) 283
BtsIMutI CAGTG 2 cut(s) 139, 220
Cac8I GCNNGC 1 cut(s) 495
CaiI CAGNNNCTG 1 cut(s) 493
CfoI GCGC 3 cut(s) 120, 351, 363
Cfr13I GGNCC 2 cut(s) 21, 257
CseI GACGC 1 cut(s) 197
Csp6I GTAC 1 cut(s) 407
CviAII CATG 4 cut(s) 147, 352, 365, 535
CviQI GTAC 1 cut(s) 407
DdeI CTNAG 4 cut(s) 17, 114, 486, 498
DpnI GATC 2 cut(s) 161, 238
DpnII GATC 2 cut(s) 159, 236
Eam1104I CTCTTC 3 cut(s) 323, 386, 513
EarI CTCTTC 3 cut(s) 323, 386, 513
Eco130I CCWWGG 2 cut(s) 146, 534
Eco24I GRGCYC 1 cut(s) 487
Eco47I GGWCC 2 cut(s) 21, 257
EcoO109I RGGNCCY 1 cut(s) 21
EcoRII CCWGG 2 cut(s) 253, 283
EcoT14I CCWWGG 2 cut(s) 146, 534
EcoT22I ATGCAT 1 cut(s) 370
EcoT38I GRGCYC 1 cut(s) 487
ErhI CCWWGG 2 cut(s) 146, 534
FaeI CATG 4 cut(s) 150, 355, 368, 538
FaiI YATR 8 cut(s) 77, 148, 245, 262, 264, 353, 366, 536
FaqI GGGAC 2 cut(s) 113, 195
FatI CATG 4 cut(s) 146, 351, 364, 534
FauNDI CATATG 1 cut(s) 262
Fnu4HI GCNGC 6 cut(s) 90, 121, 359, 459, 503, 506
FokI GGATG 1 cut(s) 290
FriOI GRGCYC 1 cut(s) 487
Fsp4HI GCNGC 6 cut(s) 90, 121, 359, 459, 503, 506
FspBI CTAG 1 cut(s) 303
GlaI GCGC 3 cut(s) 119, 350, 362
GluI GCNGC 6 cut(s) 90, 121, 359, 459, 503, 506
GsaI CCCAGC 1 cut(s) 270
HaeII RGCGCY 2 cut(s) 121, 364
HgaI GACGC 1 cut(s) 197
HhaI GCGC 3 cut(s) 120, 351, 363
Hin1I GRCGYC 1 cut(s) 208
Hin1II CATG 4 cut(s) 150, 355, 368, 538
Hin6I GCGC 3 cut(s) 118, 349, 361
HinP1I GCGC 3 cut(s) 118, 349, 361
HincII GTYRAC 1 cut(s) 32
HindII GTYRAC 1 cut(s) 32
HphI GGTGA 1 cut(s) 299
Hpy166II GTNNAC 3 cut(s) 32, 103, 290
Hpy188I TCNGA 3 cut(s) 159, 382, 489
Hpy188III TCNNGA 4 cut(s) 42, 163, 199, 275
Hpy8I GTNNAC 3 cut(s) 32, 103, 290
Hpy99I CGWCG 1 cut(s) 231
HpyAV CCTTC 1 cut(s) 311
HpyCH4V TGCA 4 cut(s) 103, 368, 467, 505
HpyF10VI GCNNNNNNNGC 3 cut(s) 355, 464, 499
HpyF3I CTNAG 4 cut(s) 17, 114, 486, 498
Hsp92I GRCGYC 1 cut(s) 208
Hsp92II CATG 4 cut(s) 150, 355, 368, 538
HspAI GCGC 3 cut(s) 118, 349, 361
Kzo9I GATC 2 cut(s) 159, 236
LmnI GCTCC 3 cut(s) 11, 77, 148
Lsp1109I GCAGC 4 cut(s) 76, 370, 489, 517
MaeI CTAG 1 cut(s) 303
MalI GATC 2 cut(s) 161, 238
MboI GATC 2 cut(s) 159, 236
MboII GAAGA 7 cut(s) 146, 340, 355, 403, 530, 535, 538
MflI RGATCY 1 cut(s) 236
MhlI GDGCHC 3 cut(s) 16, 105, 487
MluCI AATT 2 cut(s) 51, 441
MmeI TCCRAC 1 cut(s) 492
MnlI CCTC 9 cut(s) 73, 234, 261, 324, 327, 348, 387, 483, 508
Mph1103I ATGCAT 1 cut(s) 370
MseI TTAA 2 cut(s) 435, 541
MspR9I CCNGG 2 cut(s) 255, 285
MvaI CCWGG 2 cut(s) 255, 285
MwoI GCNNNNNNNGC 3 cut(s) 355, 464, 499
NcoI CCATGG 2 cut(s) 146, 534
NdeI CATATG 1 cut(s) 262
NdeII GATC 2 cut(s) 159, 236
NlaIII CATG 4 cut(s) 150, 355, 368, 538
NlaIV GGNNCC 1 cut(s) 144
NsiI ATGCAT 1 cut(s) 370
PflMI CCANNNNNTGG 1 cut(s) 266
PfoI TCCNGGA 1 cut(s) 253
PkrI GCNGC 6 cut(s) 91, 122, 360, 460, 504, 507
PpuMI RGGWCCY 1 cut(s) 21
Psp5II RGGWCCY 1 cut(s) 21
Psp6I CCWGG 2 cut(s) 253, 283
PspFI CCCAGC 1 cut(s) 266
PspGI CCWGG 2 cut(s) 253, 283
PspN4I GGNNCC 1 cut(s) 144
PspPI GGNCC 2 cut(s) 21, 257
PspPPI RGGWCCY 1 cut(s) 21
PstI CTGCAG 1 cut(s) 507
PstNI CAGNNNCTG 1 cut(s) 493
PsuI RGATCY 1 cut(s) 236
RsaI GTAC 1 cut(s) 408
RsaNI GTAC 1 cut(s) 407
SaqAI TTAA 2 cut(s) 435, 541
SatI GCNGC 6 cut(s) 90, 121, 359, 459, 503, 506
Sau3AI GATC 2 cut(s) 159, 236
Sau96I GGNCC 2 cut(s) 21, 257
ScrFI CCNGG 2 cut(s) 255, 285
SduI GDGCHC 3 cut(s) 16, 105, 487
SetI ASST 9 cut(s) 23, 65, 108, 115, 182, 253, 322, 403, 504
SfcI CTRYAG 1 cut(s) 503
SinI GGWCC 2 cut(s) 21, 257
Sse9I AATT 2 cut(s) 51, 441
SsiI CCGC 2 cut(s) 121, 458
SspMI CTAG 1 cut(s) 303
StyD4I CCNGG 2 cut(s) 253, 283
StyI CCWWGG 2 cut(s) 146, 534
TaqI TCGA 2 cut(s) 65, 229
TasI AATT 2 cut(s) 51, 441
TauI GCSGC 2 cut(s) 123, 461
Tru1I TTAA 2 cut(s) 435, 541
Tru9I TTAA 2 cut(s) 435, 541
TscAI CASTG 2 cut(s) 139, 220
TseI GCWGC 4 cut(s) 89, 358, 502, 505
TspDTI ATGAA 1 cut(s) 330
TspRI CASTG 2 cut(s) 139, 220
Van91I CCANNNNNTGG 1 cut(s) 266
VneI GTGCAC 1 cut(s) 101
VpaK11BI GGWCC 2 cut(s) 21, 257
XspI CTAG 1 cut(s) 303
Zsp2I ATGCAT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.