Rorug06G0111400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000840
Physical Location & Seq
Forward (+)
15095826 .. 15098387
2562 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0111400.1

Sequence Viewer

Length: 858 bp
ATGAAGGGAGTGAGAAAGTTAAGTCGAAAGATCTTCAAAAACGTTTGCAAACGTGATGGAGATCTCTGGAGATCATACGAGGACGCTTGCAAGAACGAAAAGGATTTCCTAACTGAACTGGACAAGTTCCTAGAGTCGTATAAGAAATGTTTGATTCAAATTAATGACGACGGAGGAATTGATGACTGTTACATGAGGATTTTGGAGAAATTGGAAAATGTCAAGGGGGCCTCAGGTAGAGTCTATGATCAGCAATTTGCACATCAATTTTTGCTCAAAAAGCTTGAATGTTTGATGACGCAGCGAAAAGATTTGCAGAATGAATTGAAAGACCGAATAGGAAAGCCTCGCAAGAAGTATGATTGTTCTCGTTCTTGGAGACGCTGTTTGAGGACGGTGACTCATATATTGTTCATTGGTGCTGCAGCTGCTGAGTTTGCCTGCACAGTTGTGGCCGCAGTTATGGCTGCTCCACTTGTTGCACTGGCCATTGCTGCTGCTATTATCCCAACACTAGCCGGACAGCATTGGACTGTGTCTTGGATTGAAGATTCTGAAATTCCTTTAGAGTATTCTGACTCTGTAATTCGCTTAATGCAATCCGGAACTGTGCTTTCCATTAAGGAATTGGGAGAAATTCGGACGGATCTCCGTGGAGTTGTACGGGACATAGAATCACTCTTATCCGATCTCAATTCTGTAATTGAGGAAGGTGAAGATATCAATATGAATAGTACTATTGAGCTTCTGGAGGACATTAATGAGTTGAAGGAAACAGCAAGGAATAGCAAGTCCAAGATATTGAAGGCAAGCAATGAGGTTTCTGAGAAGATAAAGGAGTTTTCCGAAGCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

285

Amino Acids

32.4

Weight (kDa)

6.08

Isoelectric Point (pI)

44.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 58 - 277 1.1e-15 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 602
AciI CCGC 1 cut(s) 456
AclI AACGTT 1 cut(s) 42
AclWI GGATC 1 cut(s) 654
AcoI YGGCCR 2 cut(s) 453, 486
AcsI RAATTY 2 cut(s) 558, 636
AfaI GTAC 2 cut(s) 663, 736
AgsI TTSAA 7 cut(s) 37, 158, 287, 328, 548, 769, 805
AleI CACNNNNGTG 1 cut(s) 449
AluBI AGCT 3 cut(s) 283, 428, 745
AluI AGCT 3 cut(s) 283, 428, 745
Alw26I GTCTC 1 cut(s) 373
AlwI GGATC 1 cut(s) 654
AlwNI CAGNNNCTG 1 cut(s) 431
Aor13HI TCCGGA 1 cut(s) 602
AoxI GGCC 3 cut(s) 228, 453, 486
ApeKI GCWGC 7 cut(s) 301, 422, 425, 428, 467, 494, 497
ApoI RAATTY 2 cut(s) 558, 636
AseI ATTAAT 2 cut(s) 162, 759
AspS9I GGNCC 1 cut(s) 228
AsuHPI GGTGA 2 cut(s) 409, 725
AxyI CCTNAGG 1 cut(s) 232
BalI TGGCCA 1 cut(s) 488
BbvI GCAGC 7 cut(s) 313, 409, 415, 437, 454, 481, 484
BccI CCATC 1 cut(s) 50
BclI TGATCA 1 cut(s) 247
BcoDI GTCTC 1 cut(s) 373
BfaI CTAG 2 cut(s) 131, 515
BfmI CTRYAG 1 cut(s) 423
BglII AGATCT 2 cut(s) 30, 61
BisI GCNGC 8 cut(s) 302, 423, 426, 429, 456, 468, 495, 498
BlsI GCNGC 8 cut(s) 303, 424, 427, 430, 457, 469, 496, 499
BmcAI AGTACT 1 cut(s) 736
BmgT120I GGNCC 1 cut(s) 228
BmiI GGNNCC 1 cut(s) 229
BpmI CTGGAG 2 cut(s) 88, 770
BsaBI GATNNNNATC 1 cut(s) 60
BsaJI CCNNGG 1 cut(s) 652
BsaWI WCCGGW 1 cut(s) 602
BsaXI ACNNNNNCTCC 2 cut(s) 370, 400
Bse1I ACTGG 2 cut(s) 123, 489
Bse21I CCTNAGG 1 cut(s) 232
Bse3DI GCAATG 2 cut(s) 489, 820
Bse8I GATNNNNATC 1 cut(s) 60
BseAI TCCGGA 1 cut(s) 602
BseDI CCNNGG 1 cut(s) 652
BseJI GATNNNNATC 1 cut(s) 60
BseMI GCAATG 2 cut(s) 489, 820
BseMII CTCAG 3 cut(s) 246, 423, 816
BseNI ACTGG 2 cut(s) 123, 489
BseXI GCAGC 7 cut(s) 313, 409, 415, 437, 454, 481, 484
BsgI GTGCAG 1 cut(s) 427
BshFI GGCC 3 cut(s) 230, 455, 488
BsiSI CCGG 2 cut(s) 519, 603
BslFI GGGAC 1 cut(s) 680
BsmAI GTCTC 1 cut(s) 373
BsmBI CGTCTC 1 cut(s) 373
BsmFI GGGAC 1 cut(s) 680
BsnI GGCC 3 cut(s) 230, 455, 488
Bsp13I TCCGGA 1 cut(s) 602
Bsp143I GATC 6 cut(s) 30, 61, 71, 247, 646, 688
BspACI CCGC 1 cut(s) 456
BspANI GGCC 3 cut(s) 230, 455, 488
BspCNI CTCAG 3 cut(s) 245, 424, 817
BspEI TCCGGA 1 cut(s) 602
BspLI GGNNCC 1 cut(s) 229
BspMAI CTGCAG 1 cut(s) 427
BspPI GGATC 1 cut(s) 654
BsrDI GCAATG 2 cut(s) 489, 820
BsrI ACTGG 2 cut(s) 123, 489
BssECI CCNNGG 1 cut(s) 652
BssMI GATC 6 cut(s) 30, 61, 71, 247, 646, 688
Bst4CI ACNGT 5 cut(s) 188, 397, 448, 535, 610
BstC8I GCNNGC 3 cut(s) 88, 442, 811
BstDEI CTNAG 3 cut(s) 232, 432, 825
BstDSI CCRYGG 1 cut(s) 652
BstKTI GATC 6 cut(s) 33, 64, 74, 250, 649, 691
BstMAI GTCTC 1 cut(s) 373
BstMBI GATC 6 cut(s) 30, 61, 71, 247, 646, 688
BstMWI GCNNNNNNNGC 5 cut(s) 280, 428, 437, 464, 494
BstSFI CTRYAG 1 cut(s) 423
BstV1I GCAGC 7 cut(s) 313, 409, 415, 437, 454, 481, 484
BstX2I RGATCY 3 cut(s) 30, 61, 646
BstYI RGATCY 3 cut(s) 30, 61, 646
Bsu36I CCTNAGG 1 cut(s) 232
BsuRI GGCC 3 cut(s) 230, 455, 488
BtgI CCRYGG 1 cut(s) 652
BtsIMutI CAGTG 1 cut(s) 482
Cac8I GCNNGC 3 cut(s) 88, 442, 811
CaiI CAGNNNCTG 1 cut(s) 431
Cfr13I GGNCC 1 cut(s) 228
CseI GACGC 3 cut(s) 92, 307, 390
Csp6I GTAC 2 cut(s) 662, 735
CviAII CATG 1 cut(s) 193
CviJI RGCY 9 cut(s) 230, 283, 346, 428, 455, 467, 488, 518, 745
CviKI_1 RGCY 9 cut(s) 230, 283, 346, 428, 455, 467, 488, 518, 745
CviQI GTAC 2 cut(s) 662, 735
DdeI CTNAG 3 cut(s) 232, 432, 825
DpnI GATC 6 cut(s) 32, 63, 73, 249, 648, 690
DpnII GATC 6 cut(s) 30, 61, 71, 247, 646, 688
EaeI YGGCCR 2 cut(s) 453, 486
Eco32I GATATC 1 cut(s) 721
Eco81I CCTNAGG 1 cut(s) 232
EcoO109I RGGNCCY 1 cut(s) 228
EcoRV GATATC 1 cut(s) 721
Esp3I CGTCTC 1 cut(s) 373
FaeI CATG 1 cut(s) 196
FaqI GGGAC 1 cut(s) 680
FatI CATG 1 cut(s) 192
FbaI TGATCA 1 cut(s) 247
Fnu4HI GCNGC 8 cut(s) 302, 423, 426, 429, 456, 468, 495, 498
Fsp4HI GCNGC 8 cut(s) 302, 423, 426, 429, 456, 468, 495, 498
FspBI CTAG 2 cut(s) 131, 515
GluI GCNGC 8 cut(s) 302, 423, 426, 429, 456, 468, 495, 498
GsuI CTGGAG 2 cut(s) 88, 770
HaeIII GGCC 3 cut(s) 230, 455, 488
HapII CCGG 2 cut(s) 519, 603
HgaI GACGC 3 cut(s) 92, 307, 390
Hin1II CATG 1 cut(s) 196
HindIII AAGCTT 1 cut(s) 281
HinfI GANTC 7 cut(s) 134, 154, 240, 400, 551, 578, 674
HpaII CCGG 2 cut(s) 519, 603
HphI GGTGA 2 cut(s) 409, 725
Hpy188I TCNGA 6 cut(s) 556, 577, 642, 688, 826, 847
Hpy188III TCNNGA 3 cut(s) 67, 603, 749
Hpy99I CGWCG 1 cut(s) 173
HpyAV CCTTC 3 cut(s) 704, 763, 799
HpyCH4III ACNGT 5 cut(s) 188, 397, 448, 535, 610
HpyCH4IV ACGT 2 cut(s) 42, 52
HpyCH4V TGCA 8 cut(s) 48, 90, 260, 316, 425, 444, 482, 598
HpyF10VI GCNNNNNNNGC 5 cut(s) 280, 428, 437, 464, 494
HpyF3I CTNAG 3 cut(s) 232, 432, 825
HpySE526I ACGT 2 cut(s) 42, 52
Hsp92II CATG 1 cut(s) 196
Kpn2I TCCGGA 1 cut(s) 602
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 6 cut(s) 30, 61, 71, 247, 646, 688
LmnI GCTCC 1 cut(s) 475
LpnPI CCDG 8 cut(s) 52, 104, 219, 454, 470, 532, 616, 734
Lsp1109I GCAGC 7 cut(s) 313, 409, 415, 437, 454, 481, 484
MaeI CTAG 2 cut(s) 131, 515
MaeII ACGT 2 cut(s) 42, 52
MaeIII GTNAC 2 cut(s) 188, 397
MalI GATC 6 cut(s) 32, 63, 73, 249, 648, 690
MboI GATC 6 cut(s) 30, 61, 71, 247, 646, 688
MboII GAAGA 4 cut(s) 25, 560, 728, 841
MflI RGATCY 3 cut(s) 30, 61, 646
MlsI TGGCCA 1 cut(s) 488
MluNI TGGCCA 1 cut(s) 488
MlyI GAGTC 4 cut(s) 143, 249, 394, 572
MnlI CCTC 9 cut(s) 73, 167, 189, 241, 357, 384, 700, 745, 811
Mox20I TGGCCA 1 cut(s) 488
MroI TCCGGA 1 cut(s) 602
MscI TGGCCA 1 cut(s) 488
MseI TTAA 5 cut(s) 20, 162, 593, 621, 759
MslI CAYNNNNRTG 1 cut(s) 449
Msp20I TGGCCA 1 cut(s) 488
MspA1I CMGCKG 1 cut(s) 428
MspI CCGG 2 cut(s) 519, 603
MwoI GCNNNNNNNGC 5 cut(s) 280, 428, 437, 464, 494
NdeII GATC 6 cut(s) 30, 61, 71, 247, 646, 688
NlaIII CATG 1 cut(s) 196
NlaIV GGNNCC 1 cut(s) 229
NmuCI GTSAC 1 cut(s) 397
OliI CACNNNNGTG 1 cut(s) 449
PfeI GAWTC 3 cut(s) 154, 551, 674
PflFI GACNNNGTC 1 cut(s) 535
PkrI GCNGC 8 cut(s) 303, 424, 427, 430, 457, 469, 496, 499
PleI GAGTC 4 cut(s) 142, 248, 394, 572
PpsI GAGTC 4 cut(s) 142, 248, 394, 572
PshBI ATTAAT 2 cut(s) 162, 759
Psp1406I AACGTT 1 cut(s) 42
PspN4I GGNNCC 1 cut(s) 229
PspPI GGNCC 1 cut(s) 228
PstI CTGCAG 1 cut(s) 427
PstNI CAGNNNCTG 1 cut(s) 431
PsuI RGATCY 3 cut(s) 30, 61, 646
PsyI GACNNNGTC 1 cut(s) 535
PvuII CAGCTG 1 cut(s) 428
RsaI GTAC 2 cut(s) 663, 736
RsaNI GTAC 2 cut(s) 662, 735
RseI CAYNNNNRTG 1 cut(s) 449
SaqAI TTAA 5 cut(s) 20, 162, 593, 621, 759
SatI GCNGC 8 cut(s) 302, 423, 426, 429, 456, 468, 495, 498
Sau3AI GATC 6 cut(s) 30, 61, 71, 247, 646, 688
Sau96I GGNCC 1 cut(s) 228
ScaI AGTACT 1 cut(s) 736
SchI GAGTC 4 cut(s) 143, 249, 394, 572
SetI ASST 8 cut(s) 45, 55, 238, 285, 430, 715, 747, 822
SfcI CTRYAG 1 cut(s) 423
SmiMI CAYNNNNRTG 1 cut(s) 449
SsiI CCGC 1 cut(s) 456
SspMI CTAG 2 cut(s) 131, 515
TaaI ACNGT 5 cut(s) 188, 397, 448, 535, 610
TaiI ACGT 2 cut(s) 45, 55
TaqI TCGA 1 cut(s) 25
TaqII GACCGA 1 cut(s) 348
TatI WGTACW 1 cut(s) 734
TauI GCSGC 1 cut(s) 458
TfiI GAWTC 3 cut(s) 154, 551, 674
Tru1I TTAA 5 cut(s) 20, 162, 593, 621, 759
Tru9I TTAA 5 cut(s) 20, 162, 593, 621, 759
TscAI CASTG 1 cut(s) 489
TseFI GTSAC 1 cut(s) 397
TseI GCWGC 7 cut(s) 301, 422, 425, 428, 467, 494, 497
Tsp45I GTSAC 1 cut(s) 397
TspDTI ATGAA 4 cut(s) 17, 336, 403, 743
TspGWI ACGGA 3 cut(s) 186, 641, 659
TspRI CASTG 1 cut(s) 489
Tth111I GACNNNGTC 1 cut(s) 535
VspI ATTAAT 2 cut(s) 162, 759
XapI RAATTY 2 cut(s) 558, 636
XcmI CCANNNNNNNNNTGG 1 cut(s) 625
XspI CTAG 2 cut(s) 131, 515
ZrmI AGTACT 1 cut(s) 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.