Rmu_sc0006199.1_g000003

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006199.1
OG0000840
Physical Location & Seq
Forward (+)
14367 .. 22069
7703 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006199.1_g000003.1.cds

Sequence Viewer

Length: 1461 bp
atgattttgtttgatctcgccgttctgctattgattttgagatacgtttggcgtgtgggacacgtcgatgatatttcaccttactgtgagaatttgggggaagctttatggatttatgtggttttcggattttcttttgccatactttgggtgactattatcattgctagcattgattcgtccgcctttatggcactggttactatctttgtggcagtaatgctgaagcccagtatcctaatcgctacacatagtggcgtgtggacgacttcgaggcaacttgctgataggaaggtggaggggttcgagaagaacataaccaagagaggtgcggtgaaaatattggggtttcgctgtgtggtctcctactactttcccgatcggtcaaacgccgaggaattcgaggactcgtctgccgcgaacgaggtcgggagctccggcgagggatcgacgtcgtcggggagtgagattgggctggaagagctgttgacagggatactggttgaagaaggggacggagatctgttgctgcagcagagcaaccgagaggaccgggttttgcagtggcttcaagcgcttgatatgcaggttatgggcgcgtgtcgcgcagacgagaggttgaagcctttgctgaagatgaatgcttcgaacgatgccgcagaaggtcggttgctggctcagttgactcaggtccctgcactctctacacgcaagtctttagatctcagccccatatacagagaacttgaaactagagcagttgcagaagaccgaacctatgtgaccactgccatccaagaagagatttctagaaatgggtatcgccatgctgccgacatgctgccgagctcttcacacgacggttccctacaatcgaccttcatcagacggcgcctcgatatacgacgtacgacctactgtccaccttcccaattgccgctggcatcagaacttccacccctgtctgcctgcgaatttgctcccttccggattccaattccattgccatcttcaacaaaatctcacctagatcatccctctttctccctctccctctccttctctctctccctctccttctctcaaatctcatttccttcatttgaatcaaactttttgcaaatccatctaatccaacaatggcgaactgtccaagagataattgagcagcaagtattgacggttgcgaagacggtggaggacaatctcgacgaggaaattacagccctagatcggctggatgacggtgatctgaaggcgctcagagatcggatgctccagcggatgaagaagatggcagagaagagcagccgttggatctcccttgaaaaaggacatgtgggaggaagtgttggtagtagtggcggtgaggggggtgttgctgcagcctttggctgcaacgtgggtagtgatggtggtggaagtggtggtagcaatggcttggatgaagtgctatggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

486

Amino Acids

53.49

Weight (kDa)

5.03

Isoelectric Point (pI)

52.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 455
Acc36I ACCTGC 1 cut(s) 577
AccB1I GGYRCC 1 cut(s) 891
AccB7I CCANNNNNTGG 1 cut(s) 147
AccII CGCG 3 cut(s) 419, 599, 606
AccIII TCCGGA 1 cut(s) 987
AciI CCGC 7 cut(s) 183, 332, 417, 657, 938, 1282, 1365
AclWI GGATC 2 cut(s) 454, 1325
AcsI RAATTY 3 cut(s) 91, 398, 974
AcuI CTGAAG 3 cut(s) 245, 653, 1274
AcyI GRCGYC 2 cut(s) 452, 892
AdeI CACNNNGTG 1 cut(s) 254
AfaI GTAC 1 cut(s) 910
AfeI AGCGCT 1 cut(s) 576
AfiI CCNNNNNNNGG 2 cut(s) 147, 1233
AflIII ACRYGT 2 cut(s) 61, 1336
AgsI TTSAA 7 cut(s) 506, 572, 622, 749, 1014, 1106, 1328
AhdI GACNNNNNGTC 1 cut(s) 918
AjiI CACGTC 1 cut(s) 64
AluBI AGCT 4 cut(s) 104, 435, 484, 849
AluI AGCT 4 cut(s) 104, 435, 484, 849
Alw21I GWGCWC 2 cut(s) 437, 851
Alw26I GTCTC 1 cut(s) 367
AlwI GGATC 2 cut(s) 454, 1325
Aor13HI TCCGGA 1 cut(s) 987
Aor51HI AGCGCT 1 cut(s) 576
ApeKI GCWGC 9 cut(s) 529, 532, 830, 841, 1168, 1308, 1382, 1385, 1395
ApoI RAATTY 3 cut(s) 91, 398, 974
AspLEI GCGC 5 cut(s) 577, 599, 608, 894, 1261
AspS9I GGNCC 2 cut(s) 550, 691
AsuC2I CCSGG 1 cut(s) 554
AsuHPI GGTGA 6 cut(s) 69, 163, 346, 1016, 1259, 1379
AsuII TTCGAA 1 cut(s) 647
AsuNHI GCTAGC 1 cut(s) 167
AvaII GGWCC 2 cut(s) 550, 691
BanI GGYRCC 1 cut(s) 891
BanII GRGCYC 2 cut(s) 437, 851
BbsI GAAGAC 2 cut(s) 774, 1196
Bbv12I GWGCWC 2 cut(s) 437, 851
BbvI GCAGC 9 cut(s) 516, 544, 817, 828, 1180, 1320, 1369, 1382, 1397
BccI CCATC 5 cut(s) 800, 1015, 1134, 1288, 1406
BceAI ACGGC 3 cut(s) 5, 905, 1296
BciVI GTATCC 2 cut(s) 245, 489
BcnI CCSGG 1 cut(s) 554
BcoDI GTCTC 1 cut(s) 367
BfaI CTAG 5 cut(s) 168, 753, 810, 1028, 1229
BfmI CTRYAG 2 cut(s) 530, 1383
BfoI RGCGCY 3 cut(s) 578, 895, 1262
BfuAI ACCTGC 1 cut(s) 577
BfuI GTATCC 2 cut(s) 245, 489
BglI GCCNNNNNGGC 1 cut(s) 191
BglII AGATCT 2 cut(s) 520, 721
Bme1390I CCNGG 1 cut(s) 554
Bme18I GGWCC 2 cut(s) 550, 691
BmeRI GACNNNNNGTC 1 cut(s) 918
BmgBI CACGTC 1 cut(s) 64
BmgT120I GGNCC 2 cut(s) 550, 691
BmiI GGNNCC 3 cut(s) 693, 865, 893
BmrFI CCNGG 1 cut(s) 554
BmrI ACTGGG 1 cut(s) 225
BmsI GCATC 3 cut(s) 643, 953, 1263
BmtI GCTAGC 1 cut(s) 171
BmuI ACTGGG 1 cut(s) 225
BoxI GACNNNNGTC 1 cut(s) 689
BpiI GAAGAC 2 cut(s) 774, 1196
BpmI CTGGAG 1 cut(s) 1262
Bpu14I TTCGAA 1 cut(s) 647
BpuMI CCSGG 1 cut(s) 554
BsaHI GRCGYC 2 cut(s) 452, 892
BsaI GGTCTC 1 cut(s) 367
BsaJI CCNNGG 1 cut(s) 393
BsaWI WCCGGW 1 cut(s) 987
BsaXI ACNNNNNCTCC 2 cut(s) 1335, 1365
Bsc4I CCNNNNNNNGG 2 cut(s) 147, 1233
Bse1I ACTGG 3 cut(s) 201, 231, 504
Bse3DI GCAATG 3 cut(s) 162, 1001, 1441
BseAI TCCGGA 1 cut(s) 987
BseDI CCNNGG 1 cut(s) 393
BseGI GGATG 6 cut(s) 792, 1033, 1246, 1278, 1290, 1450
BseLI CCNNNNNNNGG 2 cut(s) 147, 1233
BseMI GCAATG 3 cut(s) 162, 1001, 1441
BseMII CTCAG 4 cut(s) 692, 701, 739, 1276
BseNI ACTGG 3 cut(s) 201, 231, 504
BseXI GCAGC 9 cut(s) 516, 544, 817, 828, 1180, 1320, 1369, 1382, 1397
BsgI GTGCAG 1 cut(s) 681
Bsh1236I CGCG 3 cut(s) 419, 599, 606
Bsh1285I CGRYCG 1 cut(s) 382
BshNI GGYRCC 1 cut(s) 891
BsiEI CGRYCG 1 cut(s) 382
BsiHKAI GWGCWC 2 cut(s) 437, 851
BsiSI CCGG 3 cut(s) 438, 553, 988
BsiWI CGTACG 1 cut(s) 908
BslFI GGGAC 3 cut(s) 72, 527, 677
BslI CCNNNNNNNGG 2 cut(s) 147, 1233
BsmAI GTCTC 1 cut(s) 367
BsmFI GGGAC 3 cut(s) 72, 527, 677
BsmI GAATGC 1 cut(s) 646
Bso31I GGTCTC 1 cut(s) 367
Bsp119I TTCGAA 1 cut(s) 647
Bsp1286I GDGCHC 2 cut(s) 437, 851
Bsp13I TCCGGA 1 cut(s) 987
BspACI CCGC 7 cut(s) 183, 332, 417, 657, 938, 1282, 1365
BspCNI CTCAG 4 cut(s) 691, 700, 738, 1275
BspEI TCCGGA 1 cut(s) 987
BspFNI CGCG 3 cut(s) 419, 599, 606
BspLI GGNNCC 3 cut(s) 693, 865, 893
BspMAI CTGCAG 2 cut(s) 534, 1387
BspMI ACCTGC 1 cut(s) 577
BspOI GCTAGC 1 cut(s) 171
BspPI GGATC 2 cut(s) 454, 1325
BspQI GCTCTTC 3 cut(s) 474, 856, 1298
BspT104I TTCGAA 1 cut(s) 647
BspT107I GGYRCC 1 cut(s) 891
BspTNI GGTCTC 1 cut(s) 367
BsrDI GCAATG 3 cut(s) 162, 1001, 1441
BsrI ACTGG 3 cut(s) 201, 231, 504
BssECI CCNNGG 1 cut(s) 393
BssNI GRCGYC 2 cut(s) 452, 892
Bst4CI ACNGT 7 cut(s) 86, 863, 920, 1150, 1183, 1195, 1247
Bst6I CTCTTC 4 cut(s) 474, 795, 856, 1298
BstACI GRCGYC 2 cut(s) 452, 892
BstBI TTCGAA 1 cut(s) 647
BstC8I GCNNGC 4 cut(s) 169, 675, 942, 970
BstDEI CTNAG 4 cut(s) 678, 687, 725, 1262
BstF5I GGATG 6 cut(s) 792, 1033, 1246, 1278, 1290, 1450
BstFNI CGCG 3 cut(s) 419, 599, 606
BstH2I RGCGCY 3 cut(s) 578, 895, 1262
BstHHI GCGC 5 cut(s) 577, 599, 608, 894, 1261
BstMAI GTCTC 1 cut(s) 367
BstMCI CGRYCG 1 cut(s) 382
BstMWI GCNNNNNNNGC 6 cut(s) 191, 481, 574, 583, 603, 605
BstNSI RCATGY 2 cut(s) 841, 1340
BstPAI GACNNNNGTC 1 cut(s) 689
BstSCI CCNGG 1 cut(s) 552
BstSFI CTRYAG 2 cut(s) 530, 1383
BstUI CGCG 3 cut(s) 419, 599, 606
BstV1I GCAGC 9 cut(s) 516, 544, 817, 828, 1180, 1320, 1369, 1382, 1397
BstV2I GAAGAC 2 cut(s) 774, 1196
BstX2I RGATCY 3 cut(s) 520, 721, 1317
BstYI RGATCY 3 cut(s) 520, 721, 1317
BsuI GTATCC 2 cut(s) 245, 489
BtrI CACGTC 1 cut(s) 64
BtsCI GGATG 6 cut(s) 792, 1033, 1246, 1278, 1290, 1450
BtsI GCAGTG 2 cut(s) 569, 786
BtsIMutI CAGTG 3 cut(s) 194, 569, 786
BveI ACCTGC 1 cut(s) 577
Cac8I GCNNGC 4 cut(s) 169, 675, 942, 970
CfoI GCGC 5 cut(s) 577, 599, 608, 894, 1261
Cfr13I GGNCC 2 cut(s) 550, 691
Csp6I GTAC 1 cut(s) 909
CviAII CATG 3 cut(s) 827, 838, 1337
CviQI GTAC 1 cut(s) 909
DdeI CTNAG 4 cut(s) 678, 687, 725, 1262
DinI GGCGCC 1 cut(s) 893
DraIII CACNNNGTG 1 cut(s) 254
DriI GACNNNNNGTC 1 cut(s) 918
Eam1104I CTCTTC 4 cut(s) 474, 795, 856, 1298
Eam1105I GACNNNNNGTC 1 cut(s) 918
EarI CTCTTC 4 cut(s) 474, 795, 856, 1298
EciI GGCGGA 1 cut(s) 172
Ecl136II GAGCTC 2 cut(s) 435, 849
Eco24I GRGCYC 2 cut(s) 437, 851
Eco31I GGTCTC 1 cut(s) 367
Eco47I GGWCC 2 cut(s) 550, 691
Eco47III AGCGCT 1 cut(s) 576
Eco53kI GAGCTC 2 cut(s) 435, 849
Eco57I CTGAAG 3 cut(s) 245, 653, 1274
EcoICRI GAGCTC 2 cut(s) 435, 849
EcoO109I RGGNCCY 1 cut(s) 691
EcoRI GAATTC 1 cut(s) 398
EcoT38I GRGCYC 2 cut(s) 437, 851
EgeI GGCGCC 1 cut(s) 893
EheI GGCGCC 1 cut(s) 893
FaeI CATG 3 cut(s) 830, 841, 1340
FaqI GGGAC 3 cut(s) 72, 527, 677
FatI CATG 3 cut(s) 826, 837, 1336
FokI GGATG 6 cut(s) 779, 1020, 1253, 1285, 1297, 1457
FriOI GRGCYC 2 cut(s) 437, 851
FspBI CTAG 5 cut(s) 168, 753, 810, 1028, 1229
GlaI GCGC 5 cut(s) 576, 598, 607, 893, 1260
GsuI CTGGAG 1 cut(s) 1262
HaeII RGCGCY 3 cut(s) 578, 895, 1262
HapII CCGG 3 cut(s) 438, 553, 988
HhaI GCGC 5 cut(s) 577, 599, 608, 894, 1261
Hin1I GRCGYC 2 cut(s) 452, 892
Hin1II CATG 3 cut(s) 830, 841, 1340
Hin6I GCGC 5 cut(s) 575, 597, 606, 892, 1259
HinP1I GCGC 5 cut(s) 575, 597, 606, 892, 1259
HincII GTYRAC 2 cut(s) 489, 684
HindII GTYRAC 2 cut(s) 489, 684
HindIII AAGCTT 1 cut(s) 102
HinfI GANTC 5 cut(s) 176, 407, 685, 991, 1106
HpaII CCGG 3 cut(s) 438, 553, 988
HphI GGTGA 6 cut(s) 69, 163, 346, 1016, 1259, 1379
Hpy166II GTNNAC 4 cut(s) 264, 489, 684, 923
Hpy188I TCNGA 6 cut(s) 128, 887, 949, 1254, 1265, 1272
Hpy188III TCNNGA 6 cut(s) 307, 377, 430, 810, 988, 1208
Hpy8I GTNNAC 4 cut(s) 264, 489, 684, 923
Hpy99I CGWCG 7 cut(s) 68, 454, 457, 460, 863, 909, 1214
HpyCH4III ACNGT 7 cut(s) 86, 863, 920, 1150, 1183, 1195, 1247
HpyCH4IV ACGT 5 cut(s) 45, 63, 452, 907, 1401
HpyCH4V TGCA 8 cut(s) 532, 562, 586, 698, 764, 1120, 1385, 1398
HpyF10VI GCNNNNNNNGC 6 cut(s) 191, 481, 574, 583, 603, 605
HpyF3I CTNAG 4 cut(s) 678, 687, 725, 1262
HpySE526I ACGT 5 cut(s) 45, 63, 452, 907, 1401
Hsp92I GRCGYC 2 cut(s) 452, 892
Hsp92II CATG 3 cut(s) 830, 841, 1340
HspAI GCGC 5 cut(s) 575, 597, 606, 892, 1259
KasI GGCGCC 1 cut(s) 891
Kpn2I TCCGGA 1 cut(s) 987
LguI GCTCTTC 3 cut(s) 474, 856, 1298
LmnI GCTCC 4 cut(s) 432, 440, 985, 1281
Lsp1109I GCAGC 9 cut(s) 516, 544, 817, 828, 1180, 1320, 1369, 1382, 1397
LweI GCATC 3 cut(s) 643, 953, 1263
MaeI CTAG 5 cut(s) 168, 753, 810, 1028, 1229
MaeII ACGT 5 cut(s) 45, 63, 452, 907, 1401
MaeIII GTNAC 3 cut(s) 151, 199, 781
MfeI CAATTG 1 cut(s) 932
MflI RGATCY 3 cut(s) 520, 721, 1317
MhlI GDGCHC 2 cut(s) 437, 851
MluCI AATT 7 cut(s) 91, 398, 932, 974, 996, 1161, 1218
Mly113I GGCGCC 1 cut(s) 892
MlyI GAGTC 2 cut(s) 401, 679
MmeI TCCRAC 2 cut(s) 1159, 1295
MroI TCCGGA 1 cut(s) 987
MslI CAYNNNNRTG 1 cut(s) 66
MspA1I CMGCKG 2 cut(s) 940, 1282
MspI CCGG 3 cut(s) 438, 553, 988
MspR9I CCNGG 1 cut(s) 554
MunI CAATTG 1 cut(s) 932
Mva1269I GAATGC 1 cut(s) 646
MvnI CGCG 3 cut(s) 419, 599, 606
MwoI GCNNNNNNNGC 6 cut(s) 191, 481, 574, 583, 603, 605
NarI GGCGCC 1 cut(s) 892
NciI CCSGG 1 cut(s) 554
NheI GCTAGC 1 cut(s) 167
NlaIII CATG 3 cut(s) 830, 841, 1340
NlaIV GGNNCC 3 cut(s) 693, 865, 893
NmeAIII GCCGAG 2 cut(s) 418, 870
NmuCI GTSAC 2 cut(s) 151, 781
NspI RCATGY 2 cut(s) 841, 1340
NspV TTCGAA 1 cut(s) 647
PciI ACATGT 1 cut(s) 1336
PciSI GCTCTTC 3 cut(s) 474, 856, 1298
PcsI WCGNNNNNNNCGW 1 cut(s) 416
PctI GAATGC 1 cut(s) 646
PfeI GAWTC 3 cut(s) 176, 991, 1106
Pfl23II CGTACG 1 cut(s) 908
PflFI GACNNNGTC 1 cut(s) 454
PflMI CCANNNNNTGG 1 cut(s) 147
Ple19I CGATCG 1 cut(s) 382
PleI GAGTC 2 cut(s) 401, 679
PluTI GGCGCC 1 cut(s) 895
PpsI GAGTC 2 cut(s) 401, 679
PpuMI RGGWCCY 1 cut(s) 691
PscI ACATGT 1 cut(s) 1336
PshAI GACNNNNGTC 1 cut(s) 689
Psp124BI GAGCTC 2 cut(s) 437, 851
Psp5II RGGWCCY 1 cut(s) 691
PspLI CGTACG 1 cut(s) 908
PspN4I GGNNCC 3 cut(s) 693, 865, 893
PspPI GGNCC 2 cut(s) 550, 691
PspPPI RGGWCCY 1 cut(s) 691
PstI CTGCAG 2 cut(s) 534, 1387
PsuI RGATCY 3 cut(s) 520, 721, 1317
PsyI GACNNNGTC 1 cut(s) 454
PvuI CGATCG 1 cut(s) 382
RsaI GTAC 1 cut(s) 910
RsaNI GTAC 1 cut(s) 909
RseI CAYNNNNRTG 1 cut(s) 66
SacI GAGCTC 2 cut(s) 437, 851
SapI GCTCTTC 3 cut(s) 474, 856, 1298
Sau96I GGNCC 2 cut(s) 550, 691
SchI GAGTC 2 cut(s) 401, 679
ScrFI CCNGG 1 cut(s) 554
SduI GDGCHC 2 cut(s) 437, 851
SfaNI GCATC 3 cut(s) 643, 953, 1263
SfcI CTRYAG 2 cut(s) 530, 1383
SfoI GGCGCC 1 cut(s) 893
SfuI TTCGAA 1 cut(s) 647
SinI GGWCC 2 cut(s) 550, 691
SmiMI CAYNNNNRTG 1 cut(s) 66
Sse9I AATT 7 cut(s) 91, 398, 932, 974, 996, 1161, 1218
SsiI CCGC 7 cut(s) 183, 332, 417, 657, 938, 1282, 1365
SspDI GGCGCC 1 cut(s) 891
SspI AATATT 1 cut(s) 342
SspMI CTAG 5 cut(s) 168, 753, 810, 1028, 1229
SstI GAGCTC 2 cut(s) 437, 851
StyD4I CCNGG 1 cut(s) 552
TaaI ACNGT 7 cut(s) 86, 863, 920, 1150, 1183, 1195, 1247
TaiI ACGT 5 cut(s) 48, 66, 455, 910, 1404
TaqI TCGA 9 cut(s) 66, 272, 306, 402, 449, 647, 875, 897, 1209
TaqII GACCGA 2 cut(s) 372, 786
TasI AATT 7 cut(s) 91, 398, 932, 974, 996, 1161, 1218
TauI GCSGC 3 cut(s) 419, 659, 940
TfiI GAWTC 3 cut(s) 176, 991, 1106
TscAI CASTG 3 cut(s) 201, 569, 793
TseFI GTSAC 2 cut(s) 151, 781
TseI GCWGC 9 cut(s) 529, 532, 830, 841, 1168, 1308, 1382, 1385, 1395
Tsp45I GTSAC 2 cut(s) 151, 781
TspDTI ATGAA 5 cut(s) 653, 871, 1089, 1301, 1461
TspGWI ACGGA 1 cut(s) 531
TspRI CASTG 3 cut(s) 201, 569, 793
Tth111I GACNNNGTC 1 cut(s) 454
Van91I CCANNNNNTGG 1 cut(s) 147
VpaK11BI GGWCC 2 cut(s) 550, 691
XapI RAATTY 3 cut(s) 91, 398, 974
XbaI TCTAGA 1 cut(s) 809
XceI RCATGY 2 cut(s) 841, 1340
XspI CTAG 5 cut(s) 168, 753, 810, 1028, 1229
ZraI GACGTC 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.