Rh1DG153100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000840
Physical Location & Seq
Reverse (-)
32025543 .. 32026925
1383 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG153100.1

Sequence Viewer

Length: 486 bp
ATGAATGCACAGACGACTTCGAGGCAACTTGCTGATAGAAAGGTGGAGGGGTTCGAGAAGAACATAACCAAGAGAGGTGCGGTGAAAATATTGGGGTTTCGCTGTGTGGTCTCCTACTACTTTCCCGATCGGTCAAACACCGAGGAATTCGAGGACTCGTCTGCCGCGAACGAGGTCGGGAGCTCCGGCGAGGGATCGACGTCGTCGGGGAGTGAGATTGGGCTGGAAGAGCTGTTGACAGGGATACTGGTTGAAGGAGGGGACGGAGATCTGTTGCTGCAGCAGAGCAACCGAGAGGACCGGGTTTTGCAGTGGCTTCAAGCGCTTGATATGCAGGTTATGGGCGCGTGTCGCGCAGACGAGAGGTTGAAGCCTTTGCTGAAGATGAATGCTTCGAACGATGCCGCAGAAGGTCGGTTGCTGGCTCAGTTGACTCAGGTATATGCTATAAACCACGATCCAAGAGAATTGAGTTATAGAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

161

Amino Acids

17.77

Weight (kDa)

4.81

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 203
Acc36I ACCTGC 1 cut(s) 325
AccII CGCG 3 cut(s) 167, 347, 354
AciI CCGC 3 cut(s) 80, 165, 405
AclWI GGATC 2 cut(s) 202, 452
AcsI RAATTY 1 cut(s) 146
AcuI CTGAAG 1 cut(s) 401
AcyI GRCGYC 1 cut(s) 200
AfeI AGCGCT 1 cut(s) 324
AgsI TTSAA 3 cut(s) 254, 320, 370
AluBI AGCT 2 cut(s) 183, 232
AluI AGCT 2 cut(s) 183, 232
Alw21I GWGCWC 1 cut(s) 185
Alw26I GTCTC 1 cut(s) 115
AlwI GGATC 2 cut(s) 202, 452
Aor51HI AGCGCT 1 cut(s) 324
ApeKI GCWGC 2 cut(s) 277, 280
ApoI RAATTY 1 cut(s) 146
AspLEI GCGC 3 cut(s) 325, 347, 356
AspS9I GGNCC 1 cut(s) 298
AsuC2I CCSGG 1 cut(s) 302
AsuHPI GGTGA 1 cut(s) 94
AsuII TTCGAA 1 cut(s) 395
AvaII GGWCC 1 cut(s) 298
BanII GRGCYC 1 cut(s) 185
Bbv12I GWGCWC 1 cut(s) 185
BbvI GCAGC 2 cut(s) 264, 292
BciVI GTATCC 1 cut(s) 237
BcnI CCSGG 1 cut(s) 302
BcoDI GTCTC 1 cut(s) 115
BfmI CTRYAG 1 cut(s) 278
BfoI RGCGCY 1 cut(s) 326
BfuAI ACCTGC 1 cut(s) 325
BfuI GTATCC 1 cut(s) 237
BglII AGATCT 1 cut(s) 268
BisI GCNGC 4 cut(s) 165, 278, 281, 405
BlsI GCNGC 4 cut(s) 166, 279, 282, 406
Bme1390I CCNGG 1 cut(s) 302
Bme18I GGWCC 1 cut(s) 298
BmgT120I GGNCC 1 cut(s) 298
BmrFI CCNGG 1 cut(s) 302
BmsI GCATC 1 cut(s) 391
Bpu14I TTCGAA 1 cut(s) 395
BpuMI CCSGG 1 cut(s) 302
BsaHI GRCGYC 1 cut(s) 200
BsaI GGTCTC 1 cut(s) 115
BsaJI CCNNGG 1 cut(s) 141
Bse1I ACTGG 1 cut(s) 252
BseDI CCNNGG 1 cut(s) 141
BseMII CTCAG 2 cut(s) 440, 449
BseNI ACTGG 1 cut(s) 252
BseXI GCAGC 2 cut(s) 264, 292
Bsh1236I CGCG 3 cut(s) 167, 347, 354
Bsh1285I CGRYCG 1 cut(s) 130
BsiEI CGRYCG 1 cut(s) 130
BsiHKAI GWGCWC 1 cut(s) 185
BsiSI CCGG 2 cut(s) 186, 301
BslFI GGGAC 1 cut(s) 275
BsmAI GTCTC 1 cut(s) 115
BsmFI GGGAC 1 cut(s) 275
BsmI GAATGC 2 cut(s) 10, 394
Bso31I GGTCTC 1 cut(s) 115
Bsp119I TTCGAA 1 cut(s) 395
Bsp1286I GDGCHC 1 cut(s) 185
Bsp143I GATC 4 cut(s) 127, 194, 268, 457
BspACI CCGC 3 cut(s) 80, 165, 405
BspCNI CTCAG 2 cut(s) 439, 448
BspFNI CGCG 3 cut(s) 167, 347, 354
BspMAI CTGCAG 1 cut(s) 282
BspMI ACCTGC 1 cut(s) 325
BspPI GGATC 2 cut(s) 202, 452
BspQI GCTCTTC 1 cut(s) 222
BspT104I TTCGAA 1 cut(s) 395
BspTNI GGTCTC 1 cut(s) 115
BsrI ACTGG 1 cut(s) 252
BssECI CCNNGG 1 cut(s) 141
BssMI GATC 4 cut(s) 127, 194, 268, 457
BssNI GRCGYC 1 cut(s) 200
Bst6I CTCTTC 1 cut(s) 222
BstACI GRCGYC 1 cut(s) 200
BstBI TTCGAA 1 cut(s) 395
BstC8I GCNNGC 1 cut(s) 423
BstDEI CTNAG 2 cut(s) 426, 435
BstFNI CGCG 3 cut(s) 167, 347, 354
BstH2I RGCGCY 1 cut(s) 326
BstHHI GCGC 3 cut(s) 325, 347, 356
BstKTI GATC 4 cut(s) 130, 197, 271, 460
BstMAI GTCTC 1 cut(s) 115
BstMBI GATC 4 cut(s) 127, 194, 268, 457
BstMCI CGRYCG 1 cut(s) 130
BstMWI GCNNNNNNNGC 5 cut(s) 229, 322, 331, 351, 353
BstSCI CCNGG 1 cut(s) 300
BstSFI CTRYAG 1 cut(s) 278
BstUI CGCG 3 cut(s) 167, 347, 354
BstV1I GCAGC 2 cut(s) 264, 292
BstX2I RGATCY 1 cut(s) 268
BstYI RGATCY 1 cut(s) 268
BsuI GTATCC 1 cut(s) 237
BtsI GCAGTG 1 cut(s) 317
BtsIMutI CAGTG 1 cut(s) 317
BveI ACCTGC 1 cut(s) 325
Cac8I GCNNGC 1 cut(s) 423
CfoI GCGC 3 cut(s) 325, 347, 356
Cfr13I GGNCC 1 cut(s) 298
CviJI RGCY 6 cut(s) 183, 223, 232, 316, 373, 425
CviKI_1 RGCY 6 cut(s) 183, 223, 232, 316, 373, 425
DdeI CTNAG 2 cut(s) 426, 435
DpnI GATC 4 cut(s) 129, 196, 270, 459
DpnII GATC 4 cut(s) 127, 194, 268, 457
Eam1104I CTCTTC 1 cut(s) 222
EarI CTCTTC 1 cut(s) 222
Ecl136II GAGCTC 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 185
Eco31I GGTCTC 1 cut(s) 115
Eco47I GGWCC 1 cut(s) 298
Eco47III AGCGCT 1 cut(s) 324
Eco53kI GAGCTC 1 cut(s) 183
Eco57I CTGAAG 1 cut(s) 401
EcoICRI GAGCTC 1 cut(s) 183
EcoRI GAATTC 1 cut(s) 146
EcoT38I GRGCYC 1 cut(s) 185
FaiI YATR 7 cut(s) 65, 332, 341, 442, 444, 449, 477
FaqI GGGAC 1 cut(s) 275
Fnu4HI GCNGC 4 cut(s) 165, 278, 281, 405
FriOI GRGCYC 1 cut(s) 185
Fsp4HI GCNGC 4 cut(s) 165, 278, 281, 405
GlaI GCGC 3 cut(s) 324, 346, 355
GluI GCNGC 4 cut(s) 165, 278, 281, 405
HaeII RGCGCY 1 cut(s) 326
HapII CCGG 2 cut(s) 186, 301
HhaI GCGC 3 cut(s) 325, 347, 356
Hin1I GRCGYC 1 cut(s) 200
Hin6I GCGC 3 cut(s) 323, 345, 354
HinP1I GCGC 3 cut(s) 323, 345, 354
HincII GTYRAC 2 cut(s) 237, 432
HindII GTYRAC 2 cut(s) 237, 432
HinfI GANTC 2 cut(s) 155, 433
HpaII CCGG 2 cut(s) 186, 301
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 2 cut(s) 237, 432
Hpy188III TCNNGA 3 cut(s) 55, 125, 178
Hpy8I GTNNAC 2 cut(s) 237, 432
Hpy99I CGWCG 3 cut(s) 202, 205, 208
HpyAV CCTTC 2 cut(s) 248, 404
HpyCH4IV ACGT 1 cut(s) 200
HpyCH4V TGCA 4 cut(s) 8, 280, 310, 334
HpyF10VI GCNNNNNNNGC 5 cut(s) 229, 322, 331, 351, 353
HpyF3I CTNAG 2 cut(s) 426, 435
HpySE526I ACGT 1 cut(s) 200
Hsp92I GRCGYC 1 cut(s) 200
HspAI GCGC 3 cut(s) 323, 345, 354
Kzo9I GATC 4 cut(s) 127, 194, 268, 457
LguI GCTCTTC 1 cut(s) 222
LmnI GCTCC 2 cut(s) 180, 188
LpnPI CCDG 8 cut(s) 199, 209, 225, 233, 314, 320, 407, 422
Lsp1109I GCAGC 2 cut(s) 264, 292
LweI GCATC 1 cut(s) 391
MaeII ACGT 1 cut(s) 200
MalI GATC 4 cut(s) 129, 196, 270, 459
MboI GATC 4 cut(s) 127, 194, 268, 457
MboII GAAGA 3 cut(s) 70, 239, 394
MflI RGATCY 1 cut(s) 268
MhlI GDGCHC 1 cut(s) 185
MluCI AATT 3 cut(s) 146, 467, 481
MlyI GAGTC 2 cut(s) 149, 427
MspI CCGG 2 cut(s) 186, 301
MspR9I CCNGG 1 cut(s) 302
Mva1269I GAATGC 2 cut(s) 10, 394
MvnI CGCG 3 cut(s) 167, 347, 354
MwoI GCNNNNNNNGC 5 cut(s) 229, 322, 331, 351, 353
NciI CCSGG 1 cut(s) 302
NdeII GATC 4 cut(s) 127, 194, 268, 457
NspV TTCGAA 1 cut(s) 395
PciSI GCTCTTC 1 cut(s) 222
PcsI WCGNNNNNNNCGW 1 cut(s) 164
PctI GAATGC 2 cut(s) 10, 394
PflFI GACNNNGTC 1 cut(s) 202
PkrI GCNGC 4 cut(s) 166, 279, 282, 406
Ple19I CGATCG 1 cut(s) 130
PleI GAGTC 2 cut(s) 149, 427
PpsI GAGTC 2 cut(s) 149, 427
Psp124BI GAGCTC 1 cut(s) 185
PspPI GGNCC 1 cut(s) 298
PstI CTGCAG 1 cut(s) 282
PsuI RGATCY 1 cut(s) 268
PsyI GACNNNGTC 1 cut(s) 202
PvuI CGATCG 1 cut(s) 130
SacI GAGCTC 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 222
SatI GCNGC 4 cut(s) 165, 278, 281, 405
Sau3AI GATC 4 cut(s) 127, 194, 268, 457
Sau96I GGNCC 1 cut(s) 298
SchI GAGTC 2 cut(s) 149, 427
ScrFI CCNGG 1 cut(s) 302
SduI GDGCHC 1 cut(s) 185
SfaNI GCATC 1 cut(s) 391
SfcI CTRYAG 1 cut(s) 278
SfuI TTCGAA 1 cut(s) 395
SinI GGWCC 1 cut(s) 298
Sse9I AATT 3 cut(s) 146, 467, 481
SsiI CCGC 3 cut(s) 80, 165, 405
SspI AATATT 1 cut(s) 90
SstI GAGCTC 1 cut(s) 185
StyD4I CCNGG 1 cut(s) 300
TaiI ACGT 1 cut(s) 203
TaqI TCGA 5 cut(s) 20, 54, 150, 197, 395
TaqII GACCGA 1 cut(s) 120
TasI AATT 3 cut(s) 146, 467, 481
TauI GCSGC 2 cut(s) 167, 407
TscAI CASTG 1 cut(s) 317
TseI GCWGC 2 cut(s) 277, 280
TspDTI ATGAA 2 cut(s) 17, 401
TspGWI ACGGA 1 cut(s) 279
TspRI CASTG 1 cut(s) 317
Tth111I GACNNNGTC 1 cut(s) 202
VpaK11BI GGWCC 1 cut(s) 298
XapI RAATTY 1 cut(s) 146
ZraI GACGTC 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.