Rh4DG158900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000840
Physical Location & Seq
Forward (+)
31656300 .. 31658603
2304 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG158900.1

Sequence Viewer

Length: 732 bp
ATGAATGCACAGACGACTTCGAGGCAACTTGCTGATAGGAAGGTGGAGGGGTTCGAGAAGAACATAACCAAGAGAGGTGCGGTGAAAATATTGGGGTTTCGTTGTGTGGTCTCCTACTACTTTCCCGATCGGTCAAACGCTGAGGAATTCGAGGACTCGTCGGCCGCGAACGAGGTCGGGAGCTCCGGCGAGGGATCGACGTCGTCGGGGAGTGAGATTGGGCTGGAAGAGCTGTTGACAGGGATACTGGTTGAAGAAGGGGACGGAGATCTGTTGCTGCAGCAGAGCAACCGAGAGGACCGGGTTTTGCAGTGGCTTCAAGCGCTTGATATGCAGGTTATGGGTGCGTGTCGCGCAGACGAGAGGTTGAAGCCTTTGCTGAAGATGAATGCTTCGAACGATGCCGCAGAAGGTCGGTTGCTGGCTCAGTTGACTCAGGATACCCATGTGGAGGCACCTGAAGAGTTCTCAAAGAAGGCTACACCGTTCTTTGCTCATGTTGCTGATAAGTTTTATGCCAAAGTCAATGATGATGTTTATTTAAATATTGGTCAGTATTACATGGACTCATTTTTCAATAGGATTTGTTTATGTACTAGCTCTGCTTCCACACAATTGCTACCCCCTCCCTTTGTTTTAGATGTTCTGGGAGCTGCACTTGCAACTCATTTAGACAAGCCTTGCATTTATATTGGGTGCATGCAGTCGGGCGAAGTTTTCTCTGAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

243

Amino Acids

26.77

Weight (kDa)

4.64

Isoelectric Point (pI)

49.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 203
Acc36I ACCTGC 1 cut(s) 325
AccB1I GGYRCC 1 cut(s) 454
AccII CGCG 2 cut(s) 167, 354
AciI CCGC 3 cut(s) 80, 165, 405
AclWI GGATC 1 cut(s) 202
AcoI YGGCCR 1 cut(s) 162
AcsI RAATTY 1 cut(s) 146
AcuI CTGAAG 2 cut(s) 401, 480
AcyI GRCGYC 1 cut(s) 200
AfaI GTAC 1 cut(s) 595
AfeI AGCGCT 1 cut(s) 324
AfiI CCNNNNNNNGG 1 cut(s) 451
AgsI TTSAA 4 cut(s) 254, 320, 370, 577
AluBI AGCT 5 cut(s) 183, 232, 600, 653, 727
AluI AGCT 5 cut(s) 183, 232, 600, 653, 727
Alw21I GWGCWC 1 cut(s) 185
Alw26I GTCTC 1 cut(s) 115
AlwI GGATC 1 cut(s) 202
Aor51HI AGCGCT 1 cut(s) 324
AoxI GGCC 1 cut(s) 162
ApeKI GCWGC 3 cut(s) 277, 280, 653
ApoI RAATTY 1 cut(s) 146
AspLEI GCGC 2 cut(s) 325, 356
AspS9I GGNCC 1 cut(s) 298
AsuC2I CCSGG 1 cut(s) 302
AsuHPI GGTGA 1 cut(s) 94
AsuII TTCGAA 1 cut(s) 395
AvaII GGWCC 1 cut(s) 298
BaeI ACNNNNGTAYC 2 cut(s) 432, 465
BanI GGYRCC 1 cut(s) 454
BanII GRGCYC 1 cut(s) 185
Bbv12I GWGCWC 1 cut(s) 185
BbvCI CCTCAGC 1 cut(s) 141
BbvI GCAGC 3 cut(s) 264, 292, 640
BciVI GTATCC 2 cut(s) 237, 433
BcnI CCSGG 1 cut(s) 302
BcoDI GTCTC 1 cut(s) 115
BfaI CTAG 1 cut(s) 597
BfmI CTRYAG 1 cut(s) 278
BfoI RGCGCY 1 cut(s) 326
BfuAI ACCTGC 1 cut(s) 325
BfuI GTATCC 2 cut(s) 237, 433
BglII AGATCT 1 cut(s) 268
BisI GCNGC 5 cut(s) 165, 278, 281, 405, 654
BlsI GCNGC 5 cut(s) 166, 279, 282, 406, 655
Bme1390I CCNGG 1 cut(s) 302
Bme18I GGWCC 1 cut(s) 298
BmgT120I GGNCC 1 cut(s) 298
BmiI GGNNCC 1 cut(s) 456
BmrFI CCNGG 1 cut(s) 302
BmsI GCATC 1 cut(s) 391
Bpu10I CCTNAGC 1 cut(s) 141
Bpu14I TTCGAA 1 cut(s) 395
BpuMI CCSGG 1 cut(s) 302
BsaHI GRCGYC 1 cut(s) 200
BsaI GGTCTC 1 cut(s) 115
Bsc4I CCNNNNNNNGG 1 cut(s) 451
Bse1I ACTGG 1 cut(s) 252
BseLI CCNNNNNNNGG 1 cut(s) 451
BseMII CTCAG 4 cut(s) 132, 440, 449, 714
BseNI ACTGG 1 cut(s) 252
BseX3I CGGCCG 1 cut(s) 162
BseXI GCAGC 3 cut(s) 264, 292, 640
BsgI GTGCAG 1 cut(s) 639
Bsh1236I CGCG 2 cut(s) 167, 354
Bsh1285I CGRYCG 2 cut(s) 130, 165
BshFI GGCC 1 cut(s) 164
BshNI GGYRCC 1 cut(s) 454
BsiEI CGRYCG 2 cut(s) 130, 165
BsiHKAI GWGCWC 1 cut(s) 185
BsiSI CCGG 2 cut(s) 186, 301
BslFI GGGAC 1 cut(s) 275
BslI CCNNNNNNNGG 1 cut(s) 451
BsmAI GTCTC 1 cut(s) 115
BsmFI GGGAC 1 cut(s) 275
BsmI GAATGC 2 cut(s) 10, 394
BsnI GGCC 1 cut(s) 164
Bso31I GGTCTC 1 cut(s) 115
Bsp119I TTCGAA 1 cut(s) 395
Bsp1286I GDGCHC 1 cut(s) 185
Bsp143I GATC 3 cut(s) 127, 194, 268
BspACI CCGC 3 cut(s) 80, 165, 405
BspANI GGCC 1 cut(s) 164
BspCNI CTCAG 4 cut(s) 133, 439, 448, 715
BspFNI CGCG 2 cut(s) 167, 354
BspLI GGNNCC 1 cut(s) 456
BspMAI CTGCAG 1 cut(s) 282
BspMI ACCTGC 1 cut(s) 325
BspPI GGATC 1 cut(s) 202
BspQI GCTCTTC 1 cut(s) 222
BspT104I TTCGAA 1 cut(s) 395
BspT107I GGYRCC 1 cut(s) 454
BspTNI GGTCTC 1 cut(s) 115
BsrI ACTGG 1 cut(s) 252
BssMI GATC 3 cut(s) 127, 194, 268
BssNI GRCGYC 1 cut(s) 200
Bst4CI ACNGT 1 cut(s) 486
Bst6I CTCTTC 2 cut(s) 222, 456
BstACI GRCGYC 1 cut(s) 200
BstBI TTCGAA 1 cut(s) 395
BstC8I GCNNGC 2 cut(s) 423, 701
BstDEI CTNAG 4 cut(s) 141, 426, 435, 723
BstFNI CGCG 2 cut(s) 167, 354
BstH2I RGCGCY 1 cut(s) 326
BstHHI GCGC 2 cut(s) 325, 356
BstKTI GATC 3 cut(s) 130, 197, 271
BstMAI GTCTC 1 cut(s) 115
BstMBI GATC 3 cut(s) 127, 194, 268
BstMCI CGRYCG 2 cut(s) 130, 165
BstMWI GCNNNNNNNGC 6 cut(s) 229, 322, 331, 353, 500, 659
BstNSI RCATGY 1 cut(s) 703
BstSCI CCNGG 1 cut(s) 300
BstSFI CTRYAG 1 cut(s) 278
BstUI CGCG 2 cut(s) 167, 354
BstV1I GCAGC 3 cut(s) 264, 292, 640
BstX2I RGATCY 1 cut(s) 268
BstYI RGATCY 1 cut(s) 268
BstZI CGGCCG 1 cut(s) 162
BsuI GTATCC 2 cut(s) 237, 433
BsuRI GGCC 1 cut(s) 164
BtsI GCAGTG 1 cut(s) 317
BtsIMutI CAGTG 1 cut(s) 317
BveI ACCTGC 1 cut(s) 325
Cac8I GCNNGC 2 cut(s) 423, 701
CfoI GCGC 2 cut(s) 325, 356
Cfr13I GGNCC 1 cut(s) 298
Csp6I GTAC 1 cut(s) 594
CviAII CATG 4 cut(s) 446, 497, 562, 700
CviQI GTAC 1 cut(s) 594
DdeI CTNAG 4 cut(s) 141, 426, 435, 723
DpnI GATC 3 cut(s) 129, 196, 270
DpnII GATC 3 cut(s) 127, 194, 268
DraI TTTAAA 1 cut(s) 543
EaeI YGGCCR 1 cut(s) 162
EagI CGGCCG 1 cut(s) 162
Eam1104I CTCTTC 2 cut(s) 222, 456
EarI CTCTTC 2 cut(s) 222, 456
Ecl136II GAGCTC 1 cut(s) 183
EclXI CGGCCG 1 cut(s) 162
Eco24I GRGCYC 1 cut(s) 185
Eco31I GGTCTC 1 cut(s) 115
Eco47I GGWCC 1 cut(s) 298
Eco47III AGCGCT 1 cut(s) 324
Eco52I CGGCCG 1 cut(s) 162
Eco53kI GAGCTC 1 cut(s) 183
Eco57I CTGAAG 2 cut(s) 401, 480
EcoICRI GAGCTC 1 cut(s) 183
EcoRI GAATTC 1 cut(s) 146
EcoT38I GRGCYC 1 cut(s) 185
FaeI CATG 4 cut(s) 449, 500, 565, 703
FaqI GGGAC 1 cut(s) 275
FatI CATG 4 cut(s) 445, 496, 561, 699
Fnu4HI GCNGC 5 cut(s) 165, 278, 281, 405, 654
FriOI GRGCYC 1 cut(s) 185
Fsp4HI GCNGC 5 cut(s) 165, 278, 281, 405, 654
FspBI CTAG 1 cut(s) 597
GlaI GCGC 2 cut(s) 324, 355
GluI GCNGC 5 cut(s) 165, 278, 281, 405, 654
HaeII RGCGCY 1 cut(s) 326
HaeIII GGCC 1 cut(s) 164
HapII CCGG 2 cut(s) 186, 301
HhaI GCGC 2 cut(s) 325, 356
Hin1I GRCGYC 1 cut(s) 200
Hin1II CATG 4 cut(s) 449, 500, 565, 703
Hin6I GCGC 2 cut(s) 323, 354
HinP1I GCGC 2 cut(s) 323, 354
HincII GTYRAC 2 cut(s) 237, 432
HindII GTYRAC 2 cut(s) 237, 432
HinfI GANTC 3 cut(s) 155, 433, 566
HpaII CCGG 2 cut(s) 186, 301
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 2 cut(s) 237, 432
Hpy188I TCNGA 1 cut(s) 724
Hpy188III TCNNGA 4 cut(s) 55, 125, 178, 437
Hpy8I GTNNAC 2 cut(s) 237, 432
Hpy99I CGWCG 4 cut(s) 163, 202, 205, 208
HpyAV CCTTC 4 cut(s) 34, 251, 404, 469
HpyCH4III ACNGT 1 cut(s) 486
HpyCH4IV ACGT 1 cut(s) 200
HpyCH4V TGCA 9 cut(s) 8, 280, 310, 334, 656, 662, 684, 699, 703
HpyF10VI GCNNNNNNNGC 6 cut(s) 229, 322, 331, 353, 500, 659
HpyF3I CTNAG 4 cut(s) 141, 426, 435, 723
HpySE526I ACGT 1 cut(s) 200
Hsp92I GRCGYC 1 cut(s) 200
Hsp92II CATG 4 cut(s) 449, 500, 565, 703
HspAI GCGC 2 cut(s) 323, 354
Kzo9I GATC 3 cut(s) 127, 194, 268
LguI GCTCTTC 1 cut(s) 222
LmnI GCTCC 3 cut(s) 180, 188, 650
Lsp1109I GCAGC 3 cut(s) 264, 292, 640
LweI GCATC 1 cut(s) 391
MaeI CTAG 1 cut(s) 597
MaeII ACGT 1 cut(s) 200
MalI GATC 3 cut(s) 129, 196, 270
MboI GATC 3 cut(s) 127, 194, 268
MboII GAAGA 5 cut(s) 70, 239, 266, 394, 473
MfeI CAATTG 1 cut(s) 614
MflI RGATCY 1 cut(s) 268
MhlI GDGCHC 1 cut(s) 185
MluCI AATT 2 cut(s) 146, 614
MlyI GAGTC 3 cut(s) 149, 427, 560
MseI TTAA 1 cut(s) 542
MspI CCGG 2 cut(s) 186, 301
MspR9I CCNGG 1 cut(s) 302
MunI CAATTG 1 cut(s) 614
Mva1269I GAATGC 2 cut(s) 10, 394
MvnI CGCG 2 cut(s) 167, 354
MwoI GCNNNNNNNGC 6 cut(s) 229, 322, 331, 353, 500, 659
NciI CCSGG 1 cut(s) 302
NdeII GATC 3 cut(s) 127, 194, 268
NlaIII CATG 4 cut(s) 449, 500, 565, 703
NlaIV GGNNCC 1 cut(s) 456
NspI RCATGY 1 cut(s) 703
NspV TTCGAA 1 cut(s) 395
PaeI GCATGC 1 cut(s) 703
PciSI GCTCTTC 1 cut(s) 222
PcsI WCGNNNNNNNCGW 1 cut(s) 164
PctI GAATGC 2 cut(s) 10, 394
PflFI GACNNNGTC 1 cut(s) 202
PkrI GCNGC 5 cut(s) 166, 279, 282, 406, 655
Ple19I CGATCG 1 cut(s) 130
PleI GAGTC 3 cut(s) 149, 427, 560
PpsI GAGTC 3 cut(s) 149, 427, 560
Psp124BI GAGCTC 1 cut(s) 185
PspN4I GGNNCC 1 cut(s) 456
PspPI GGNCC 1 cut(s) 298
PstI CTGCAG 1 cut(s) 282
PsuI RGATCY 1 cut(s) 268
PsyI GACNNNGTC 1 cut(s) 202
PvuI CGATCG 1 cut(s) 130
RsaI GTAC 1 cut(s) 595
RsaNI GTAC 1 cut(s) 594
SacI GAGCTC 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 222
SaqAI TTAA 1 cut(s) 542
SatI GCNGC 5 cut(s) 165, 278, 281, 405, 654
Sau3AI GATC 3 cut(s) 127, 194, 268
Sau96I GGNCC 1 cut(s) 298
SchI GAGTC 3 cut(s) 149, 427, 560
ScrFI CCNGG 1 cut(s) 302
SduI GDGCHC 1 cut(s) 185
SfaNI GCATC 1 cut(s) 391
SfcI CTRYAG 1 cut(s) 278
SfuI TTCGAA 1 cut(s) 395
SinI GGWCC 1 cut(s) 298
SmiI ATTTAAAT 1 cut(s) 543
SphI GCATGC 1 cut(s) 703
Sse9I AATT 2 cut(s) 146, 614
SsiI CCGC 3 cut(s) 80, 165, 405
SspI AATATT 2 cut(s) 90, 547
SspMI CTAG 1 cut(s) 597
SstI GAGCTC 1 cut(s) 185
StyD4I CCNGG 1 cut(s) 300
SwaI ATTTAAAT 1 cut(s) 543
TaaI ACNGT 1 cut(s) 486
TaiI ACGT 1 cut(s) 203
TaqI TCGA 5 cut(s) 20, 54, 150, 197, 395
TaqII GACCGA 1 cut(s) 120
TasI AATT 2 cut(s) 146, 614
TatI WGTACW 1 cut(s) 593
TauI GCSGC 2 cut(s) 167, 407
Tru1I TTAA 1 cut(s) 542
Tru9I TTAA 1 cut(s) 542
TscAI CASTG 1 cut(s) 317
TseI GCWGC 3 cut(s) 277, 280, 653
TspDTI ATGAA 2 cut(s) 17, 401
TspGWI ACGGA 1 cut(s) 279
TspRI CASTG 1 cut(s) 317
Tth111I GACNNNGTC 1 cut(s) 202
VpaK11BI GGWCC 1 cut(s) 298
XapI RAATTY 1 cut(s) 146
XceI RCATGY 1 cut(s) 703
XspI CTAG 1 cut(s) 597
ZraI GACGTC 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.