pycom04g07310

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000840
Physical Location & Seq
Reverse (-)
7756157 .. 7756446
290 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g07310.2

Sequence Viewer

Length: 249 bp
ATGACTAACCATGGAGACCAAGATTTACACACGAAGGACGAGATCAATTGCGTTGGGGATCAATCGAACGGTAGGAATCAGAGAACTCATCGCCTTCGAACGGTCTGGGGAGCTCCGACGCTGGACCGGCGTCGAGGAGTGAGACTGGATGGAGATTTGTTGCTCCAGCAAAGCAATCGGGATGACCAAGTTTTGCAATGGCTAGAAGCGCTTGATATGCTGGTTATGGGCGCGTGTTGTGCCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

83

Amino Acids

9.34

Weight (kDa)

5.8

Isoelectric Point (pI)

46.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 233
AclWI GGATC 1 cut(s) 66
AcyI GRCGYC 1 cut(s) 130
AfeI AGCGCT 1 cut(s) 210
AfiI CCNNNNNNNGG 1 cut(s) 100
AluBI AGCT 1 cut(s) 113
AluI AGCT 1 cut(s) 113
Alw21I GWGCWC 1 cut(s) 115
Alw26I GTCTC 2 cut(s) 9, 136
AlwI GGATC 1 cut(s) 66
Aor51HI AGCGCT 1 cut(s) 210
AspLEI GCGC 2 cut(s) 211, 233
AspS9I GGNCC 1 cut(s) 124
AsuII TTCGAA 1 cut(s) 97
AvaII GGWCC 1 cut(s) 124
BanII GRGCYC 1 cut(s) 115
Bbv12I GWGCWC 1 cut(s) 115
BccI CCATC 1 cut(s) 143
BcgI CGANNNNNNTGC 2 cut(s) 158, 192
BcoDI GTCTC 2 cut(s) 9, 136
BfaI CTAG 1 cut(s) 203
BfoI RGCGCY 1 cut(s) 212
Bme18I GGWCC 1 cut(s) 124
BmgT120I GGNCC 1 cut(s) 124
BoxI GACNNNNGTC 1 cut(s) 129
BpmI CTGGAG 1 cut(s) 149
Bpu14I TTCGAA 1 cut(s) 97
BsaHI GRCGYC 1 cut(s) 130
BsaI GGTCTC 1 cut(s) 9
BsaJI CCNNGG 1 cut(s) 10
Bsc4I CCNNNNNNNGG 1 cut(s) 100
Bse118I RCCGGY 1 cut(s) 126
Bse1I ACTGG 1 cut(s) 150
Bse3DI GCAATG 1 cut(s) 203
BseDI CCNNGG 1 cut(s) 10
BseGI GGATG 2 cut(s) 154, 187
BseLI CCNNNNNNNGG 1 cut(s) 100
BseMI GCAATG 1 cut(s) 203
BseNI ACTGG 1 cut(s) 150
BseRI GAGGAG 1 cut(s) 150
Bsh1236I CGCG 1 cut(s) 233
BsiHKAI GWGCWC 1 cut(s) 115
BsiSI CCGG 1 cut(s) 127
BslI CCNNNNNNNGG 1 cut(s) 100
BsmAI GTCTC 2 cut(s) 9, 136
Bso31I GGTCTC 1 cut(s) 9
Bsp119I TTCGAA 1 cut(s) 97
Bsp1286I GDGCHC 1 cut(s) 115
Bsp143I GATC 2 cut(s) 42, 58
Bsp19I CCATGG 1 cut(s) 10
BspFNI CGCG 1 cut(s) 233
BspPI GGATC 1 cut(s) 66
BspT104I TTCGAA 1 cut(s) 97
BspTNI GGTCTC 1 cut(s) 9
BsrDI GCAATG 1 cut(s) 203
BsrFI RCCGGY 1 cut(s) 126
BsrI ACTGG 1 cut(s) 150
BssAI RCCGGY 1 cut(s) 126
BssECI CCNNGG 1 cut(s) 10
BssMI GATC 2 cut(s) 42, 58
BssNI GRCGYC 1 cut(s) 130
BssT1I CCWWGG 1 cut(s) 10
Bst4CI ACNGT 2 cut(s) 71, 103
BstACI GRCGYC 1 cut(s) 130
BstBI TTCGAA 1 cut(s) 97
BstDSI CCRYGG 1 cut(s) 10
BstF5I GGATG 2 cut(s) 154, 187
BstFNI CGCG 1 cut(s) 233
BstH2I RGCGCY 1 cut(s) 212
BstHHI GCGC 2 cut(s) 211, 233
BstKTI GATC 2 cut(s) 45, 61
BstMAI GTCTC 2 cut(s) 9, 136
BstMBI GATC 2 cut(s) 42, 58
BstMWI GCNNNNNNNGC 4 cut(s) 127, 208, 217, 239
BstPAI GACNNNNGTC 1 cut(s) 129
BstUI CGCG 1 cut(s) 233
BtgI CCRYGG 1 cut(s) 10
BtgZI GCGATG 1 cut(s) 74
BtsCI GGATG 2 cut(s) 154, 187
CfoI GCGC 2 cut(s) 211, 233
Cfr10I RCCGGY 1 cut(s) 126
Cfr13I GGNCC 1 cut(s) 124
CseI GACGC 2 cut(s) 119, 127
CviAII CATG 1 cut(s) 11
CviJI RGCY 2 cut(s) 113, 202
CviKI_1 RGCY 2 cut(s) 113, 202
DpnI GATC 2 cut(s) 44, 60
DpnII GATC 2 cut(s) 42, 58
Ecl136II GAGCTC 1 cut(s) 113
Eco130I CCWWGG 1 cut(s) 10
Eco24I GRGCYC 1 cut(s) 115
Eco31I GGTCTC 1 cut(s) 9
Eco47I GGWCC 1 cut(s) 124
Eco47III AGCGCT 1 cut(s) 210
Eco53kI GAGCTC 1 cut(s) 113
EcoICRI GAGCTC 1 cut(s) 113
EcoT14I CCWWGG 1 cut(s) 10
EcoT38I GRGCYC 1 cut(s) 115
ErhI CCWWGG 1 cut(s) 10
FaeI CATG 1 cut(s) 14
FaiI YATR 3 cut(s) 12, 218, 227
FatI CATG 1 cut(s) 10
FokI GGATG 2 cut(s) 161, 194
FriOI GRGCYC 1 cut(s) 115
FspBI CTAG 1 cut(s) 203
GlaI GCGC 2 cut(s) 210, 232
GsuI CTGGAG 1 cut(s) 149
HaeII RGCGCY 1 cut(s) 212
HapII CCGG 1 cut(s) 127
HgaI GACGC 2 cut(s) 119, 127
HhaI GCGC 2 cut(s) 211, 233
Hin1I GRCGYC 1 cut(s) 130
Hin1II CATG 1 cut(s) 14
Hin6I GCGC 2 cut(s) 209, 231
HinP1I GCGC 2 cut(s) 209, 231
HinfI GANTC 1 cut(s) 76
HpaII CCGG 1 cut(s) 127
Hpy188I TCNGA 2 cut(s) 81, 117
Hpy188III TCNNGA 1 cut(s) 179
Hpy99I CGWCG 2 cut(s) 121, 135
HpyAV CCTTC 2 cut(s) 28, 104
HpyCH4III ACNGT 2 cut(s) 71, 103
HpyCH4V TGCA 1 cut(s) 196
HpyF10VI GCNNNNNNNGC 4 cut(s) 127, 208, 217, 239
Hsp92I GRCGYC 1 cut(s) 130
Hsp92II CATG 1 cut(s) 14
HspAI GCGC 2 cut(s) 209, 231
Kzo9I GATC 2 cut(s) 42, 58
LmnI GCTCC 3 cut(s) 110, 118, 168
LpnPI CCDG 6 cut(s) 91, 107, 131, 140, 179, 206
MaeI CTAG 1 cut(s) 203
MalI GATC 2 cut(s) 44, 60
MboI GATC 2 cut(s) 42, 58
MfeI CAATTG 1 cut(s) 46
MhlI GDGCHC 1 cut(s) 115
MluCI AATT 1 cut(s) 46
MmeI TCCRAC 1 cut(s) 140
MnlI CCTC 1 cut(s) 128
MspI CCGG 1 cut(s) 127
MunI CAATTG 1 cut(s) 46
MvnI CGCG 1 cut(s) 233
MwoI GCNNNNNNNGC 4 cut(s) 127, 208, 217, 239
NcoI CCATGG 1 cut(s) 10
NdeII GATC 2 cut(s) 42, 58
NlaIII CATG 1 cut(s) 14
NspV TTCGAA 1 cut(s) 97
PfeI GAWTC 1 cut(s) 76
PshAI GACNNNNGTC 1 cut(s) 129
Psp124BI GAGCTC 1 cut(s) 115
PspPI GGNCC 1 cut(s) 124
SacI GAGCTC 1 cut(s) 115
Sau3AI GATC 2 cut(s) 42, 58
Sau96I GGNCC 1 cut(s) 124
SduI GDGCHC 1 cut(s) 115
SetI ASST 1 cut(s) 115
SfuI TTCGAA 1 cut(s) 97
SinI GGWCC 1 cut(s) 124
Sse9I AATT 1 cut(s) 46
SspMI CTAG 1 cut(s) 203
SstI GAGCTC 1 cut(s) 115
StyI CCWWGG 1 cut(s) 10
TaaI ACNGT 2 cut(s) 71, 103
TaqI TCGA 3 cut(s) 65, 97, 133
TasI AATT 1 cut(s) 46
TfiI GAWTC 1 cut(s) 76
VpaK11BI GGWCC 1 cut(s) 124
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.