Rh2BG375000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000840
Physical Location & Seq
Forward (+)
52940189 .. 52944727
4539 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG375000.1

Sequence Viewer

Length: 258 bp
ATGATGGATGAGACTGATCAAGCGATTGATAGAGCACAAAGGTTCTTCATCAGACTACCAACTGAGGACTATGACTTTGAATGCTTTTGGGTGAAGATACTAAGCTTCCTCTGCCATATCTTAAAGTCGGATATCTTACTGTACGACCGGAGAGAGAAAGCTTCTTCCTCTGAGCTCCGGTTTCTCTCTTCTTCTTCTTCTTGTTTCTGGGTCCCTGTGGTTTGGTTTGCTTGTTCTGATTTGGTTCTCAAGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

85

Amino Acids

10.11

Weight (kDa)

4.96

Isoelectric Point (pI)

72.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 143
AgsI TTSAA 1 cut(s) 80
AluBI AGCT 3 cut(s) 105, 161, 175
AluI AGCT 3 cut(s) 105, 161, 175
Alw21I GWGCWC 2 cut(s) 37, 177
Alw26I GTCTC 1 cut(s) 5
ArsI GACNNNNNNTTYG 2 cut(s) 59, 91
AspS9I GGNCC 1 cut(s) 211
AsuHPI GGTGA 1 cut(s) 103
AvaII GGWCC 1 cut(s) 211
BanII GRGCYC 1 cut(s) 177
Bbv12I GWGCWC 2 cut(s) 37, 177
BclI TGATCA 1 cut(s) 16
BcoDI GTCTC 1 cut(s) 5
BfaI CTAG 1 cut(s) 256
BglII AGATCT 1 cut(s) 252
Bme18I GGWCC 1 cut(s) 211
BmgT120I GGNCC 1 cut(s) 211
BmiI GGNNCC 2 cut(s) 212, 213
BpuEI CTTGAG 1 cut(s) 233
BsaWI WCCGGW 2 cut(s) 147, 177
BseGI GGATG 1 cut(s) 13
BseMII CTCAG 2 cut(s) 54, 162
Bsh1285I CGRYCG 1 cut(s) 148
BsiEI CGRYCG 1 cut(s) 148
BsiHKAI GWGCWC 2 cut(s) 37, 177
BsiSI CCGG 2 cut(s) 148, 178
BslFI GGGAC 1 cut(s) 197
BsmAI GTCTC 1 cut(s) 5
BsmFI GGGAC 1 cut(s) 197
BsmI GAATGC 1 cut(s) 86
Bsp1286I GDGCHC 2 cut(s) 37, 177
Bsp143I GATC 2 cut(s) 16, 252
BspCNI CTCAG 2 cut(s) 55, 163
BspLI GGNNCC 2 cut(s) 212, 213
BssMI GATC 2 cut(s) 16, 252
Bst4CI ACNGT 1 cut(s) 141
Bst6I CTCTTC 1 cut(s) 193
BstDEI CTNAG 3 cut(s) 63, 101, 171
BstF5I GGATG 1 cut(s) 13
BstKTI GATC 2 cut(s) 19, 255
BstMAI GTCTC 1 cut(s) 5
BstMBI GATC 2 cut(s) 16, 252
BstMCI CGRYCG 1 cut(s) 148
BstMWI GCNNNNNNNGC 1 cut(s) 111
BstX2I RGATCY 1 cut(s) 252
BstYI RGATCY 1 cut(s) 252
BtsCI GGATG 1 cut(s) 13
Cfr13I GGNCC 1 cut(s) 211
Csp6I GTAC 1 cut(s) 142
CviJI RGCY 3 cut(s) 105, 161, 175
CviKI_1 RGCY 3 cut(s) 105, 161, 175
CviQI GTAC 1 cut(s) 142
DdeI CTNAG 3 cut(s) 63, 101, 171
DpnI GATC 2 cut(s) 18, 254
DpnII GATC 2 cut(s) 16, 252
Eam1104I CTCTTC 1 cut(s) 193
EarI CTCTTC 1 cut(s) 193
Ecl136II GAGCTC 1 cut(s) 175
Eco24I GRGCYC 1 cut(s) 177
Eco32I GATATC 1 cut(s) 133
Eco47I GGWCC 1 cut(s) 211
Eco53kI GAGCTC 1 cut(s) 175
EcoICRI GAGCTC 1 cut(s) 175
EcoO109I RGGNCCY 1 cut(s) 211
EcoRV GATATC 1 cut(s) 133
EcoT38I GRGCYC 1 cut(s) 177
FaiI YATR 2 cut(s) 72, 117
FaqI GGGAC 1 cut(s) 197
FbaI TGATCA 1 cut(s) 16
FokI GGATG 1 cut(s) 20
FriOI GRGCYC 1 cut(s) 177
FspBI CTAG 1 cut(s) 256
HapII CCGG 2 cut(s) 148, 178
HindIII AAGCTT 2 cut(s) 103, 159
HpaII CCGG 2 cut(s) 148, 178
HphI GGTGA 1 cut(s) 103
Hpy188I TCNGA 4 cut(s) 53, 130, 172, 238
Hpy188III TCNNGA 1 cut(s) 250
HpyCH4III ACNGT 1 cut(s) 141
HpyF10VI GCNNNNNNNGC 1 cut(s) 111
HpyF3I CTNAG 3 cut(s) 63, 101, 171
KflI GGGWCCC 1 cut(s) 211
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 2 cut(s) 16, 252
LmnI GCTCC 1 cut(s) 180
LpnPI CCDG 4 cut(s) 161, 191, 193, 228
MaeI CTAG 1 cut(s) 256
MalI GATC 2 cut(s) 18, 254
MboI GATC 2 cut(s) 16, 252
MboII GAAGA 7 cut(s) 37, 106, 156, 180, 183, 186, 189
MflI RGATCY 1 cut(s) 252
MhlI GDGCHC 2 cut(s) 37, 177
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 3 cut(s) 58, 119, 178
MseI TTAA 1 cut(s) 122
MspI CCGG 2 cut(s) 148, 178
Mva1269I GAATGC 1 cut(s) 86
MwoI GCNNNNNNNGC 1 cut(s) 111
NdeII GATC 2 cut(s) 16, 252
NlaIV GGNNCC 2 cut(s) 212, 213
PctI GAATGC 1 cut(s) 86
PpuMI RGGWCCY 1 cut(s) 211
Psp124BI GAGCTC 1 cut(s) 177
Psp5II RGGWCCY 1 cut(s) 211
PspN4I GGNNCC 2 cut(s) 212, 213
PspPI GGNCC 1 cut(s) 211
PspPPI RGGWCCY 1 cut(s) 211
PsuI RGATCY 1 cut(s) 252
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
SacI GAGCTC 1 cut(s) 177
SaqAI TTAA 1 cut(s) 122
Sau3AI GATC 2 cut(s) 16, 252
Sau96I GGNCC 1 cut(s) 211
SduI GDGCHC 2 cut(s) 37, 177
SetI ASST 4 cut(s) 44, 107, 163, 177
SgeI CNNG 7 cut(s) 32, 160, 190, 213, 220, 227, 243
SinI GGWCC 1 cut(s) 211
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
SspMI CTAG 1 cut(s) 256
SstI GAGCTC 1 cut(s) 177
TaaI ACNGT 1 cut(s) 141
Tru1I TTAA 1 cut(s) 122
Tru9I TTAA 1 cut(s) 122
TspDTI ATGAA 1 cut(s) 37
VpaK11BI GGWCC 1 cut(s) 211
XspI CTAG 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.