Belongs to the glycosyltransferase 31 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AluBI | AGCT | 3 cut(s) | 100, 219, 252 |
| AluI | AGCT | 3 cut(s) | 100, 219, 252 |
| AspS9I | GGNCC | 1 cut(s) | 222 |
| AvaII | GGWCC | 1 cut(s) | 222 |
| BglII | AGATCT | 1 cut(s) | 207 |
| Bme18I | GGWCC | 1 cut(s) | 222 |
| BmgT120I | GGNCC | 1 cut(s) | 222 |
| BseGI | GGATG | 2 cut(s) | 151, 159 |
| BseMII | CTCAG | 1 cut(s) | 87 |
| BsgI | GTGCAG | 1 cut(s) | 12 |
| Bsp143I | GATC | 1 cut(s) | 207 |
| BspCNI | CTCAG | 1 cut(s) | 88 |
| BssMI | GATC | 1 cut(s) | 207 |
| BstDEI | CTNAG | 1 cut(s) | 96 |
| BstF5I | GGATG | 2 cut(s) | 151, 159 |
| BstKTI | GATC | 1 cut(s) | 210 |
| BstMBI | GATC | 1 cut(s) | 207 |
| BstX2I | RGATCY | 1 cut(s) | 207 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 120 |
| BstYI | RGATCY | 1 cut(s) | 207 |
| BtsCI | GGATG | 2 cut(s) | 151, 159 |
| Cfr13I | GGNCC | 1 cut(s) | 222 |
| CviAII | CATG | 2 cut(s) | 73, 107 |
| CviJI | RGCY | 4 cut(s) | 52, 100, 219, 252 |
| CviKI_1 | RGCY | 4 cut(s) | 52, 100, 219, 252 |
| DdeI | CTNAG | 1 cut(s) | 96 |
| DpnI | GATC | 1 cut(s) | 209 |
| DpnII | GATC | 1 cut(s) | 207 |
| Eco47I | GGWCC | 1 cut(s) | 222 |
| FaeI | CATG | 2 cut(s) | 76, 110 |
| FaiI | YATR | 4 cut(s) | 63, 74, 108, 256 |
| FatI | CATG | 2 cut(s) | 72, 106 |
| FokI | GGATG | 2 cut(s) | 158, 166 |
| Hin1II | CATG | 2 cut(s) | 76, 110 |
| Hpy188I | TCNGA | 2 cut(s) | 97, 207 |
| HpyAV | CCTTC | 2 cut(s) | 132, 172 |
| HpyCH4V | TGCA | 4 cut(s) | 29, 35, 72, 240 |
| HpyF3I | CTNAG | 1 cut(s) | 96 |
| Hsp92II | CATG | 2 cut(s) | 76, 110 |
| Kzo9I | GATC | 1 cut(s) | 207 |
| LpnPI | CCDG | 4 cut(s) | 5, 126, 196, 205 |
| MalI | GATC | 1 cut(s) | 209 |
| MboI | GATC | 1 cut(s) | 207 |
| MflI | RGATCY | 1 cut(s) | 207 |
| MluCI | AATT | 1 cut(s) | 241 |
| MnlI | CCTC | 2 cut(s) | 136, 235 |
| MseI | TTAA | 1 cut(s) | 248 |
| NdeII | GATC | 1 cut(s) | 207 |
| NlaIII | CATG | 2 cut(s) | 76, 110 |
| PspPI | GGNCC | 1 cut(s) | 222 |
| PsuI | RGATCY | 1 cut(s) | 207 |
| SaqAI | TTAA | 1 cut(s) | 248 |
| Sau3AI | GATC | 1 cut(s) | 207 |
| Sau96I | GGNCC | 1 cut(s) | 222 |
| SetI | ASST | 5 cut(s) | 102, 183, 221, 227, 254 |
| SinI | GGWCC | 1 cut(s) | 222 |
| Sse9I | AATT | 1 cut(s) | 241 |
| TasI | AATT | 1 cut(s) | 241 |
| Tru1I | TTAA | 1 cut(s) | 248 |
| Tru9I | TTAA | 1 cut(s) | 248 |
| TspDTI | ATGAA | 2 cut(s) | 89, 123 |
| VpaK11BI | GGWCC | 1 cut(s) | 222 |