Rorug06G0061700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000840
Physical Location & Seq
Reverse (-)
8247857 .. 8249430
1574 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0061700.1

Sequence Viewer

Length: 1056 bp
ATGAGTCGGAGGAGTTGGGATGGGGTGCGGAAAAAGTTTGATCCCGCCGATGCACCGCCCAAATACCAGGGCGTGGCCCTGGTACTTGGGGTTACCGGACTCGTCGGAAATAGTCTCGCTGAGATTCTTCCACTGCCCGACACTCCCGGCGGGCCGTGGAAGGTCTACGGCGTGGCCCGGCGGCCCCGGCCGCAGTGGAACGCTGACCACCCGATTCAGTACATCCAGTGTGACCTCTCTGACTCTACACAGACCGAGGCAGAGCTCTCCCAGCTCACTGATGTCACTCACATTTTTTATGTCACGTGGGCGAGCAAGCCAACTGAAGCTGAGAACTGTGAGGCTAATGGCAAGATGTTGAGCAATGTGTTGAATGCTGTGATCCCAAACGGGCCGAATTTGCAGCACATATGTATGCAGACGGGTCGGAAGCACTACTTAGGTCCGTTTGAGATGTTGGGGAATGTCCAACCCCATGAACCTCCATGTCACGAAGACCTGCCGAGGCTTAATGCCCCCAATTTTTACTATGTGATGGAGGATATTCTGTTTGAGGAGGTGAAGAAGAAGGAGGGCTTACATGGGCAGCCGCTCAGGTTTCCGGGGAGTAAAGGAACTTGGGAAGGATTCTGGGACGCTTCTGATGCGGATATGGTTGCTGAACATCAGATATGGGCAGCTGTGGAGCCTTATGCCAAGAATGAAGCTTTTAATTGCATCAATGGGGATGTGTTCAAGTGGAAGCATTTGTGGGGGATTCTGGCTGAGCAGTTCGAGCTAGTGCCTGCTGGAGTTCACGAGGAGTTGAGTTTTGAGGAGATGATGAAAGATAAAGGGCCAGTGTGGGATGAAATTGTGAGAGAGCATGGTTTGGTGCCTACCAAATTGGAAGAGGTAGGGAATTGGTGGTTTCTGGACACTATGTTTCGGTGGATTGATTCAACTGCGGACAGTATGAACAAGAGCAAAGAGCATGGTTTTGTTGGCTTTCGGAACCCGAAAACTTCCTTTGTTTCTTGGATTGACAAGATGAAGTCTTTTAGAATTGTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

351

Amino Acids

39.95

Weight (kDa)

5.41

Isoelectric Point (pI)

40.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 26 - 179 2.2e-06 NAD dependent epimerase/dehydratase family
PRISE PF22917 83 - 189 3.1e-08 PRISE-like Rossmann-fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 507
AccB1I GGYRCC 1 cut(s) 874
AccB7I CCANNNNNTGG 1 cut(s) 73
AccBSI CCGCTC 1 cut(s) 592
AccI GTMKAC 1 cut(s) 165
AciI CCGC 9 cut(s) 28, 45, 56, 150, 181, 191, 590, 647, 947
AclWI GGATC 2 cut(s) 35, 376
AcoI YGGCCR 1 cut(s) 188
AcsI RAATTY 1 cut(s) 397
AcuI CTGAAG 1 cut(s) 345
AcvI CACGTG 1 cut(s) 306
AfaI GTAC 2 cut(s) 84, 221
AfiI CCNNNNNNNGG 1 cut(s) 73
AgsI TTSAA 3 cut(s) 373, 736, 942
AjnI CCWGG 2 cut(s) 66, 78
AluBI AGCT 6 cut(s) 265, 274, 329, 680, 707, 778
AluI AGCT 6 cut(s) 265, 274, 329, 680, 707, 778
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 1 cut(s) 119
AlwI GGATC 2 cut(s) 35, 376
AoxI GGCC 7 cut(s) 75, 152, 174, 182, 188, 392, 836
ApeKI GCWGC 3 cut(s) 403, 586, 677
ApoI RAATTY 1 cut(s) 397
AspS9I GGNCC 7 cut(s) 76, 152, 175, 183, 392, 443, 836
AsuC2I CCSGG 4 cut(s) 147, 178, 187, 603
AsuHPI GGTGA 1 cut(s) 571
AvaII GGWCC 1 cut(s) 443
BanI GGYRCC 1 cut(s) 874
BanII GRGCYC 1 cut(s) 267
BauI CACGAG 1 cut(s) 797
BbrPI CACGTG 1 cut(s) 306
BbsI GAAGAC 1 cut(s) 501
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 3 cut(s) 415, 598, 689
BccI CCATC 2 cut(s) 14, 529
BceAI ACGGC 2 cut(s) 139, 184
BcgI CGANNNNNNTGC 2 cut(s) 407, 441
BciT130I CCWGG 2 cut(s) 68, 80
BcnI CCSGG 4 cut(s) 147, 178, 187, 603
BcoDI GTCTC 1 cut(s) 119
BfaI CTAG 1 cut(s) 779
BfuAI ACCTGC 1 cut(s) 507
BisI GCNGC 6 cut(s) 182, 191, 404, 587, 590, 678
BlpI GCTNAGC 1 cut(s) 765
BlsI GCNGC 6 cut(s) 183, 192, 405, 588, 591, 679
Bme1390I CCNGG 6 cut(s) 68, 80, 147, 178, 187, 603
Bme18I GGWCC 1 cut(s) 443
BmgT120I GGNCC 7 cut(s) 76, 152, 175, 183, 392, 443, 836
BmiI GGNNCC 4 cut(s) 185, 687, 876, 995
BmrFI CCNGG 6 cut(s) 68, 80, 147, 178, 187, 603
BmsI GCATC 3 cut(s) 40, 634, 726
BpiI GAAGAC 1 cut(s) 501
BpmI CTGGAG 1 cut(s) 810
Bpu10I CCTNAGC 1 cut(s) 593
Bpu1102I GCTNAGC 1 cut(s) 765
BpuMI CCSGG 4 cut(s) 147, 178, 187, 603
BsaAI YACGTR 1 cut(s) 306
BsaJI CCNNGG 7 cut(s) 67, 78, 155, 185, 255, 503, 602
BsaWI WCCGGW 1 cut(s) 95
Bsc4I CCNNNNNNNGG 1 cut(s) 73
Bse1I ACTGG 2 cut(s) 226, 839
Bse3DI GCAATG 1 cut(s) 370
BseBI CCWGG 2 cut(s) 68, 80
BseDI CCNNGG 7 cut(s) 67, 78, 155, 185, 255, 503, 602
BseGI GGATG 4 cut(s) 25, 222, 733, 853
BseLI CCNNNNNNNGG 1 cut(s) 73
BseMI GCAATG 1 cut(s) 370
BseMII CTCAG 4 cut(s) 111, 321, 607, 756
BseNI ACTGG 2 cut(s) 226, 839
BseRI GAGGAG 4 cut(s) 25, 569, 815, 830
BseX3I CGGCCG 1 cut(s) 188
BseXI GCAGC 3 cut(s) 415, 598, 689
BseYI CCCAGC 1 cut(s) 270
Bsh1285I CGRYCG 1 cut(s) 191
BshFI GGCC 7 cut(s) 77, 154, 176, 184, 190, 394, 838
BshNI GGYRCC 1 cut(s) 874
BsiEI CGRYCG 1 cut(s) 191
BsiHKAI GWGCWC 1 cut(s) 267
BsiSI CCGG 5 cut(s) 96, 147, 178, 187, 602
BslFI GGGAC 1 cut(s) 647
BslI CCNNNNNNNGG 1 cut(s) 73
BsmAI GTCTC 1 cut(s) 119
BsmFI GGGAC 1 cut(s) 647
BsmI GAATGC 1 cut(s) 379
BsnI GGCC 7 cut(s) 77, 154, 176, 184, 190, 394, 838
Bsp1286I GDGCHC 1 cut(s) 267
Bsp143I GATC 2 cut(s) 40, 381
Bsp1720I GCTNAGC 1 cut(s) 765
BspACI CCGC 9 cut(s) 28, 45, 56, 150, 181, 191, 590, 647, 947
BspANI GGCC 7 cut(s) 77, 154, 176, 184, 190, 394, 838
BspCNI CTCAG 4 cut(s) 112, 322, 606, 757
BspLI GGNNCC 4 cut(s) 185, 687, 876, 995
BspMI ACCTGC 1 cut(s) 507
BspPI GGATC 2 cut(s) 35, 376
BspT107I GGYRCC 1 cut(s) 874
BsrBI CCGCTC 1 cut(s) 592
BsrDI GCAATG 1 cut(s) 370
BsrI ACTGG 2 cut(s) 226, 839
BssECI CCNNGG 7 cut(s) 67, 78, 155, 185, 255, 503, 602
BssMI GATC 2 cut(s) 40, 381
BssSI CACGAG 1 cut(s) 797
Bst2BI CACGAG 1 cut(s) 797
Bst2UI CCWGG 2 cut(s) 68, 80
Bst4CI ACNGT 2 cut(s) 338, 953
Bst6I CTCTTC 1 cut(s) 885
BstBAI YACGTR 1 cut(s) 306
BstC8I GCNNGC 4 cut(s) 152, 313, 317, 786
BstDEI CTNAG 5 cut(s) 120, 330, 439, 593, 765
BstDSI CCRYGG 1 cut(s) 155
BstEII GGTNACC 1 cut(s) 91
BstF5I GGATG 4 cut(s) 25, 222, 733, 853
BstKTI GATC 2 cut(s) 43, 384
BstMAI GTCTC 1 cut(s) 119
BstMBI GATC 2 cut(s) 40, 381
BstMCI CGRYCG 1 cut(s) 191
BstMWI GCNNNNNNNGC 6 cut(s) 187, 190, 271, 400, 644, 775
BstNI CCWGG 2 cut(s) 68, 80
BstPI GGTNACC 1 cut(s) 91
BstSCI CCNGG 6 cut(s) 66, 78, 145, 176, 185, 601
BstV1I GCAGC 3 cut(s) 415, 598, 689
BstV2I GAAGAC 1 cut(s) 501
BstZI CGGCCG 1 cut(s) 188
BsuRI GGCC 7 cut(s) 77, 154, 176, 184, 190, 394, 838
BtgI CCRYGG 1 cut(s) 155
BtsCI GGATG 4 cut(s) 25, 222, 733, 853
BtsI GCAGTG 2 cut(s) 131, 200
BtsIMutI CAGTG 5 cut(s) 131, 200, 233, 276, 846
BveI ACCTGC 1 cut(s) 507
Cac8I GCNNGC 4 cut(s) 152, 313, 317, 786
Cfr13I GGNCC 7 cut(s) 76, 152, 175, 183, 392, 443, 836
CseI GACGC 1 cut(s) 644
Csp6I GTAC 2 cut(s) 83, 220
CviAII CATG 5 cut(s) 476, 486, 581, 866, 974
CviQI GTAC 2 cut(s) 83, 220
DdeI CTNAG 5 cut(s) 120, 330, 439, 593, 765
DpnI GATC 2 cut(s) 42, 383
DpnII GATC 2 cut(s) 40, 381
EaeI YGGCCR 1 cut(s) 188
EagI CGGCCG 1 cut(s) 188
Eam1104I CTCTTC 1 cut(s) 885
EarI CTCTTC 1 cut(s) 885
Ecl136II GAGCTC 1 cut(s) 265
EclXI CGGCCG 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 443
Eco52I CGGCCG 1 cut(s) 188
Eco53kI GAGCTC 1 cut(s) 265
Eco57I CTGAAG 1 cut(s) 345
Eco72I CACGTG 1 cut(s) 306
Eco91I GGTNACC 1 cut(s) 91
EcoICRI GAGCTC 1 cut(s) 265
EcoO65I GGTNACC 1 cut(s) 91
EcoRII CCWGG 2 cut(s) 66, 78
EcoT38I GRGCYC 1 cut(s) 267
FaeI CATG 5 cut(s) 479, 489, 584, 869, 977
FalI AAGNNNNNCTT 4 cut(s) 422, 454, 560, 592
FaqI GGGAC 1 cut(s) 647
FatI CATG 5 cut(s) 475, 485, 580, 865, 973
FauI CCCGC 2 cut(s) 52, 143
FauNDI CATATG 1 cut(s) 410
FblI GTMKAC 1 cut(s) 165
Fnu4HI GCNGC 6 cut(s) 182, 191, 404, 587, 590, 678
FokI GGATG 4 cut(s) 32, 209, 740, 860
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 6 cut(s) 182, 191, 404, 587, 590, 678
FspBI CTAG 1 cut(s) 779
GluI GCNGC 6 cut(s) 182, 191, 404, 587, 590, 678
GsaI CCCAGC 1 cut(s) 274
GsuI CTGGAG 1 cut(s) 810
HaeIII GGCC 7 cut(s) 77, 154, 176, 184, 190, 394, 838
HapII CCGG 5 cut(s) 96, 147, 178, 187, 602
HgaI GACGC 1 cut(s) 644
Hin1II CATG 5 cut(s) 479, 489, 584, 869, 977
HindIII AAGCTT 1 cut(s) 705
HinfI GANTC 8 cut(s) 4, 99, 124, 214, 242, 627, 757, 938
HpaII CCGG 5 cut(s) 96, 147, 178, 187, 602
HphI GGTGA 1 cut(s) 571
Hpy166II GTNNAC 2 cut(s) 166, 796
Hpy188I TCNGA 7 cut(s) 9, 107, 241, 429, 643, 669, 993
Hpy188III TCNNGA 3 cut(s) 491, 797, 914
Hpy8I GTNNAC 2 cut(s) 166, 796
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 3 cut(s) 154, 562, 617
HpyCH4III ACNGT 2 cut(s) 338, 953
HpyCH4IV ACGT 1 cut(s) 305
HpyCH4V TGCA 4 cut(s) 53, 403, 418, 717
HpyF10VI GCNNNNNNNGC 6 cut(s) 187, 190, 271, 400, 644, 775
HpyF3I CTNAG 5 cut(s) 120, 330, 439, 593, 765
HpySE526I ACGT 1 cut(s) 305
Hsp92II CATG 5 cut(s) 479, 489, 584, 869, 977
Kzo9I GATC 2 cut(s) 40, 381
LmnI GCTCC 1 cut(s) 685
Lsp1109I GCAGC 3 cut(s) 415, 598, 689
LweI GCATC 3 cut(s) 40, 634, 726
MaeI CTAG 1 cut(s) 779
MaeII ACGT 1 cut(s) 305
MaeIII GTNAC 5 cut(s) 91, 230, 283, 301, 488
MalI GATC 2 cut(s) 42, 383
MbiI CCGCTC 1 cut(s) 592
MboI GATC 2 cut(s) 40, 381
MboII GAAGA 5 cut(s) 119, 506, 574, 577, 902
MhlI GDGCHC 1 cut(s) 267
MluCI AATT 7 cut(s) 397, 520, 712, 852, 884, 901, 1044
MlyI GAGTC 3 cut(s) 13, 93, 236
MmeI TCCRAC 3 cut(s) 85, 407, 493
MseI TTAA 2 cut(s) 510, 711
MslI CAYNNNNRTG 1 cut(s) 413
MspA1I CMGCKG 1 cut(s) 680
MspI CCGG 5 cut(s) 96, 147, 178, 187, 602
MspR9I CCNGG 6 cut(s) 68, 80, 147, 178, 187, 603
Mva1269I GAATGC 1 cut(s) 379
MvaI CCWGG 2 cut(s) 68, 80
MwoI GCNNNNNNNGC 6 cut(s) 187, 190, 271, 400, 644, 775
NciI CCSGG 4 cut(s) 147, 178, 187, 603
NdeI CATATG 1 cut(s) 410
NdeII GATC 2 cut(s) 40, 381
NlaIII CATG 5 cut(s) 479, 489, 584, 869, 977
NlaIV GGNNCC 4 cut(s) 185, 687, 876, 995
NmeAIII GCCGAG 1 cut(s) 528
NmuCI GTSAC 4 cut(s) 230, 283, 301, 488
PctI GAATGC 1 cut(s) 379
PfeI GAWTC 5 cut(s) 124, 214, 627, 757, 938
PflMI CCANNNNNTGG 1 cut(s) 73
PkrI GCNGC 6 cut(s) 183, 192, 405, 588, 591, 679
PleI GAGTC 3 cut(s) 12, 93, 236
PmaCI CACGTG 1 cut(s) 306
PmlI CACGTG 1 cut(s) 306
PpsI GAGTC 3 cut(s) 12, 93, 236
Ppu21I YACGTR 1 cut(s) 306
Psp124BI GAGCTC 1 cut(s) 267
Psp6I CCWGG 2 cut(s) 66, 78
PspCI CACGTG 1 cut(s) 306
PspEI GGTNACC 1 cut(s) 91
PspFI CCCAGC 1 cut(s) 270
PspGI CCWGG 2 cut(s) 66, 78
PspN4I GGNNCC 4 cut(s) 185, 687, 876, 995
PspPI GGNCC 7 cut(s) 76, 152, 175, 183, 392, 443, 836
PvuII CAGCTG 1 cut(s) 680
RsaI GTAC 2 cut(s) 84, 221
RsaNI GTAC 2 cut(s) 83, 220
RseI CAYNNNNRTG 1 cut(s) 413
SacI GAGCTC 1 cut(s) 267
SaqAI TTAA 2 cut(s) 510, 711
SatI GCNGC 6 cut(s) 182, 191, 404, 587, 590, 678
Sau3AI GATC 2 cut(s) 40, 381
Sau96I GGNCC 7 cut(s) 76, 152, 175, 183, 392, 443, 836
SchI GAGTC 3 cut(s) 13, 93, 236
ScrFI CCNGG 6 cut(s) 68, 80, 147, 178, 187, 603
SduI GDGCHC 1 cut(s) 267
SfaNI GCATC 3 cut(s) 40, 634, 726
SinI GGWCC 1 cut(s) 443
SmiMI CAYNNNNRTG 1 cut(s) 413
Sse9I AATT 7 cut(s) 397, 520, 712, 852, 884, 901, 1044
SsiI CCGC 9 cut(s) 28, 45, 56, 150, 181, 191, 590, 647, 947
SspMI CTAG 1 cut(s) 779
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 6 cut(s) 66, 78, 145, 176, 185, 601
TaaI ACNGT 2 cut(s) 338, 953
TaiI ACGT 1 cut(s) 308
TaqI TCGA 1 cut(s) 774
TaqII GACCGA 1 cut(s) 269
TasI AATT 7 cut(s) 397, 520, 712, 852, 884, 901, 1044
TatI WGTACW 1 cut(s) 219
TauI GCSGC 3 cut(s) 184, 193, 592
TfiI GAWTC 5 cut(s) 124, 214, 627, 757, 938
Tru1I TTAA 2 cut(s) 510, 711
Tru9I TTAA 2 cut(s) 510, 711
TscAI CASTG 5 cut(s) 138, 200, 233, 283, 846
TseFI GTSAC 4 cut(s) 230, 283, 301, 488
TseI GCWGC 3 cut(s) 403, 586, 677
Tsp45I GTSAC 4 cut(s) 230, 283, 301, 488
TspDTI ATGAA 6 cut(s) 492, 717, 839, 864, 971, 1046
TspGWI ACGGA 1 cut(s) 435
TspRI CASTG 5 cut(s) 138, 200, 233, 283, 846
Van91I CCANNNNNTGG 1 cut(s) 73
VpaK11BI GGWCC 1 cut(s) 443
XapI RAATTY 1 cut(s) 397
XmiI GTMKAC 1 cut(s) 165
XspI CTAG 1 cut(s) 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.