pycom05g00570

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010261
Physical Location & Seq
Forward (+)
723079 .. 724427
1349 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g00570.3

Sequence Viewer

Length: 546 bp
ATGGTATTCGGAACCCTAAGGTCCATCGTGCGACCTCTATCAAGAACCCTAATATCCCGAACCTCAACTTCTTCCTTGACGTCCTTTGCTGCCCGCAATGCGTGCCCGAAACCTGAGCTCCGCTGCATTTCTGGTGGCAGCCAAGCTCCGTGGTTTTTCCCCATTTCCAACTATTTTCACAGCTTGACAGATACTCGTTTCCCCAAACGGCGTCCGACCGACAAGCCACGTCGAAAAAGGGCCAGCGTTAGACCTCCTGGACCATTGGCTGCAGTTCAGTGTGTTCCTGGTGCGCCAATAGTTCCTAATAGACCCAATGAAGGAAGTGTGAAGAGAAGGAATGAGAAGAAGCGCATGAGGCAACGTCGTGCATTTATATTGGCAGAAAAAAAGAAAAGGAAAGCTCTGGTGCAGGAGGCTAAAAGGAAGAAAGTTATAAAGAGAGTTGAGAGAAAAATGGCCGCTGTTGCAAGGGAAAGAGCATGGACTCAAAGACTTGCCGAGCTGCAGAAACTGGAAGAAGAGAAGAAAAAGTCCATAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

182

Amino Acids

20.76

Weight (kDa)

11.73

Isoelectric Point (pI)

64.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 437
AatII GACGTC 1 cut(s) 83
AciI CCGC 3 cut(s) 94, 121, 462
AcoI YGGCCR 1 cut(s) 459
AcyI GRCGYC 2 cut(s) 80, 211
AfiI CCNNNNNNNGG 1 cut(s) 320
AjiI CACGTC 1 cut(s) 230
AjnI CCWGG 2 cut(s) 256, 286
AluBI AGCT 6 cut(s) 118, 146, 183, 404, 505, 542
AluI AGCT 6 cut(s) 118, 146, 183, 404, 505, 542
Alw21I GWGCWC 1 cut(s) 120
AlwNI CAGNNNCTG 1 cut(s) 514
AoxI GGCC 2 cut(s) 240, 459
ApeKI GCWGC 5 cut(s) 89, 123, 138, 269, 505
AspLEI GCGC 2 cut(s) 295, 354
AspS9I GGNCC 3 cut(s) 21, 240, 260
AvaII GGWCC 2 cut(s) 21, 260
AxyI CCTNAGG 1 cut(s) 17
BaeGI GKGCMC 1 cut(s) 107
BanII GRGCYC 1 cut(s) 120
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 5 cut(s) 76, 110, 150, 256, 492
BccI CCATC 1 cut(s) 32
BceAI ACGGC 1 cut(s) 224
BciT130I CCWGG 2 cut(s) 258, 288
BfmI CTRYAG 2 cut(s) 270, 506
BisI GCNGC 6 cut(s) 90, 124, 139, 270, 462, 506
BlsI GCNGC 6 cut(s) 91, 125, 140, 271, 463, 507
Bme1390I CCNGG 2 cut(s) 258, 288
Bme18I GGWCC 2 cut(s) 21, 260
BmgBI CACGTC 1 cut(s) 230
BmgT120I GGNCC 3 cut(s) 21, 240, 260
BmiI GGNNCC 1 cut(s) 13
BmrFI CCNGG 2 cut(s) 258, 288
Bpu10I CCTNAGC 1 cut(s) 114
BsaHI GRCGYC 2 cut(s) 80, 211
BsaJI CCNNGG 1 cut(s) 149
BsaXI ACNNNNNCTCC 2 cut(s) 102, 132
Bsc4I CCNNNNNNNGG 1 cut(s) 320
Bse1I ACTGG 1 cut(s) 519
Bse21I CCTNAGG 1 cut(s) 17
Bse3DI GCAATG 1 cut(s) 103
BseBI CCWGG 2 cut(s) 258, 288
BseDI CCNNGG 1 cut(s) 149
BseLI CCNNNNNNNGG 1 cut(s) 320
BseMI GCAATG 1 cut(s) 103
BseMII CTCAG 1 cut(s) 105
BseNI ACTGG 1 cut(s) 519
BseSI GKGCMC 1 cut(s) 107
BseXI GCAGC 5 cut(s) 76, 110, 150, 256, 492
BsgI GTGCAG 1 cut(s) 431
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 2 cut(s) 242, 461
BsiEI CGRYCG 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 120
BslI CCNNNNNNNGG 1 cut(s) 320
BsnI GGCC 2 cut(s) 242, 461
Bsp1286I GDGCHC 2 cut(s) 107, 120
BspACI CCGC 3 cut(s) 94, 121, 462
BspANI GGCC 2 cut(s) 242, 461
BspCNI CTCAG 1 cut(s) 106
BspLI GGNNCC 1 cut(s) 13
BspMAI CTGCAG 2 cut(s) 274, 510
BsrDI GCAATG 1 cut(s) 103
BsrI ACTGG 1 cut(s) 519
BssECI CCNNGG 1 cut(s) 149
BssNI GRCGYC 2 cut(s) 80, 211
Bst2UI CCWGG 2 cut(s) 258, 288
Bst6I CTCTTC 2 cut(s) 326, 516
BstACI GRCGYC 2 cut(s) 80, 211
BstAPI GCANNNNNTGC 1 cut(s) 102
BstC8I GCNNGC 3 cut(s) 94, 103, 244
BstDEI CTNAG 3 cut(s) 17, 114, 543
BstDSI CCRYGG 1 cut(s) 149
BstHHI GCGC 2 cut(s) 295, 354
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 4 cut(s) 98, 102, 358, 467
BstNI CCWGG 2 cut(s) 258, 288
BstSCI CCNGG 2 cut(s) 256, 286
BstSFI CTRYAG 2 cut(s) 270, 506
BstSLI GKGCMC 1 cut(s) 107
BstV1I GCAGC 5 cut(s) 76, 110, 150, 256, 492
Bsu36I CCTNAGG 1 cut(s) 17
BsuRI GGCC 2 cut(s) 242, 461
BtgI CCRYGG 1 cut(s) 149
BtrI CACGTC 1 cut(s) 230
BtsIMutI CAGTG 1 cut(s) 284
Cac8I GCNNGC 3 cut(s) 94, 103, 244
CaiI CAGNNNCTG 1 cut(s) 514
CfoI GCGC 2 cut(s) 295, 354
Cfr13I GGNCC 3 cut(s) 21, 240, 260
CseI GACGC 1 cut(s) 200
CspCI CAANNNNNGTGG 2 cut(s) 131, 166
CviAII CATG 2 cut(s) 355, 483
DdeI CTNAG 3 cut(s) 17, 114, 543
EaeI YGGCCR 1 cut(s) 459
Eam1104I CTCTTC 2 cut(s) 326, 516
EarI CTCTTC 2 cut(s) 326, 516
Ecl136II GAGCTC 1 cut(s) 118
Eco24I GRGCYC 1 cut(s) 120
Eco47I GGWCC 2 cut(s) 21, 260
Eco53kI GAGCTC 1 cut(s) 118
Eco81I CCTNAGG 1 cut(s) 17
EcoICRI GAGCTC 1 cut(s) 118
EcoRII CCWGG 2 cut(s) 256, 286
EcoT38I GRGCYC 1 cut(s) 120
FaeI CATG 2 cut(s) 358, 486
FaiI YATR 5 cut(s) 356, 377, 437, 484, 539
FatI CATG 2 cut(s) 354, 482
FauI CCCGC 1 cut(s) 101
Fnu4HI GCNGC 6 cut(s) 90, 124, 139, 270, 462, 506
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 6 cut(s) 90, 124, 139, 270, 462, 506
GlaI GCGC 2 cut(s) 294, 353
GluI GCNGC 6 cut(s) 90, 124, 139, 270, 462, 506
HaeIII GGCC 2 cut(s) 242, 461
HgaI GACGC 1 cut(s) 200
HhaI GCGC 2 cut(s) 295, 354
Hin1I GRCGYC 2 cut(s) 80, 211
Hin1II CATG 2 cut(s) 358, 486
Hin6I GCGC 2 cut(s) 293, 352
HinP1I GCGC 2 cut(s) 293, 352
HinfI GANTC 1 cut(s) 487
Hpy188I TCNGA 2 cut(s) 11, 216
Hpy188III TCNNGA 2 cut(s) 42, 57
Hpy99I CGWCG 2 cut(s) 234, 369
HpyAV CCTTC 2 cut(s) 314, 330
HpyCH4IV ACGT 3 cut(s) 80, 229, 364
HpyCH4V TGCA 6 cut(s) 126, 272, 371, 412, 470, 508
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 102, 358, 467
HpyF3I CTNAG 3 cut(s) 17, 114, 543
HpySE526I ACGT 3 cut(s) 80, 229, 364
Hsp92I GRCGYC 2 cut(s) 80, 211
Hsp92II CATG 2 cut(s) 358, 486
HspAI GCGC 2 cut(s) 293, 352
LmnI GCTCC 2 cut(s) 123, 151
Lsp1109I GCAGC 5 cut(s) 76, 110, 150, 256, 492
MaeII ACGT 3 cut(s) 80, 229, 364
MboII GAAGA 7 cut(s) 63, 343, 358, 439, 530, 533, 538
MhlI GDGCHC 2 cut(s) 107, 120
MlyI GAGTC 1 cut(s) 481
MmeI TCCRAC 2 cut(s) 192, 239
MnlI CCTC 5 cut(s) 45, 73, 264, 351, 409
MspA1I CMGCKG 2 cut(s) 123, 464
MspR9I CCNGG 2 cut(s) 258, 288
MvaI CCWGG 2 cut(s) 258, 288
MwoI GCNNNNNNNGC 4 cut(s) 98, 102, 358, 467
NlaIII CATG 2 cut(s) 358, 486
NlaIV GGNNCC 1 cut(s) 13
NmeAIII GCCGAG 1 cut(s) 526
PfoI TCCNGGA 1 cut(s) 256
PkrI GCNGC 6 cut(s) 91, 125, 140, 271, 463, 507
PleI GAGTC 1 cut(s) 481
PpsI GAGTC 1 cut(s) 481
PsiI TTATAA 1 cut(s) 437
Psp124BI GAGCTC 1 cut(s) 120
Psp6I CCWGG 2 cut(s) 256, 286
PspGI CCWGG 2 cut(s) 256, 286
PspN4I GGNNCC 1 cut(s) 13
PspPI GGNCC 3 cut(s) 21, 240, 260
PstI CTGCAG 2 cut(s) 274, 510
PstNI CAGNNNCTG 1 cut(s) 514
SacI GAGCTC 1 cut(s) 120
SatI GCNGC 6 cut(s) 90, 124, 139, 270, 462, 506
Sau96I GGNCC 3 cut(s) 21, 240, 260
SchI GAGTC 1 cut(s) 481
ScrFI CCNGG 2 cut(s) 258, 288
SduI GDGCHC 2 cut(s) 107, 120
SfcI CTRYAG 2 cut(s) 270, 506
SinI GGWCC 2 cut(s) 21, 260
SsiI CCGC 3 cut(s) 94, 121, 462
SstI GAGCTC 1 cut(s) 120
StyD4I CCNGG 2 cut(s) 256, 286
TaiI ACGT 3 cut(s) 83, 232, 367
TaqI TCGA 1 cut(s) 232
TaqII GACCGA 1 cut(s) 233
TauI GCSGC 1 cut(s) 464
TscAI CASTG 1 cut(s) 284
TseI GCWGC 5 cut(s) 89, 123, 138, 269, 505
TspDTI ATGAA 1 cut(s) 333
TspGWI ACGGA 1 cut(s) 138
TspRI CASTG 1 cut(s) 284
VpaK11BI GGWCC 2 cut(s) 21, 260
ZraI GACGTC 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.