MD10G1009400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010261
Physical Location & Seq
Forward (+)
1329499 .. 1332427
2929 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1009400.v1.1.491

Sequence Viewer

Length: 543 bp
ATGGGATTCGGAGCCCTAAGGTCCATTGTGCGACCTCTATCGCGAACCATAATATCCCGAACCTCAACTTCCTCCATGACGTCCTTAGCTGCCAGCTGTGTGTGCCCCAAACCTGAACTCCCCTTCATTTCTGGTCGCCAAGCCCCATGGTTTTTTCCTGTCTCCAACTATTTTCACAGCTTGACAGATACTCGTTTTCCCAAACGTCGACCAGTAGACAAGCCACGTCGAAAAAGGGCCAGCGTAAGACCTCCAGGACCATTTGCTGCGGTTCAGTATGTTCCTGGTGAGCCAATAGTTCCTAATAGACCCAATGAAGGAAGTGTGAAGAGGAGGAATGAGAAGAAGCGCATGAGGCAACGCCGCGCATTCATATTGGCAGAAAAAAAGAAAAGGAAAGCTCTGGTGCAGGAAGCTAAAAGGAAGAAAGTTATAAAGCGAGTTGAGAGAAAAATGGCTGCTGTTGCAAGGGAAAGAGCGTGGACTCAAAGACTCGCCGAGCTGCAGAAACTGGAAGAAGAGAAGAAAAATTCCATGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

20.75

Weight (kDa)

11.61

Isoelectric Point (pI)

66.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 434
AatII GACGTC 1 cut(s) 83
AccI GTMKAC 2 cut(s) 208, 216
AccII CGCG 2 cut(s) 43, 366
AciI CCGC 2 cut(s) 269, 364
AcsI RAATTY 1 cut(s) 529
AcyI GRCGYC 1 cut(s) 80
AfiI CCNNNNNNNGG 1 cut(s) 317
AjiI CACGTC 1 cut(s) 227
AjnI CCWGG 2 cut(s) 253, 283
AluBI AGCT 6 cut(s) 89, 96, 180, 401, 416, 502
AluI AGCT 6 cut(s) 89, 96, 180, 401, 416, 502
Alw26I GTCTC 1 cut(s) 166
AlwNI CAGNNNCTG 1 cut(s) 511
AoxI GGCC 1 cut(s) 237
ApeKI GCWGC 4 cut(s) 89, 266, 458, 502
ApoI RAATTY 1 cut(s) 529
AspLEI GCGC 2 cut(s) 351, 368
AspS9I GGNCC 3 cut(s) 21, 237, 257
AsuHPI GGTGA 1 cut(s) 299
AvaII GGWCC 2 cut(s) 21, 257
AxyI CCTNAGG 1 cut(s) 17
BaeGI GKGCMC 1 cut(s) 107
BanII GRGCYC 1 cut(s) 16
BbvI GCAGC 4 cut(s) 76, 253, 445, 489
BciT130I CCWGG 2 cut(s) 255, 285
BcoDI GTCTC 1 cut(s) 166
BfmI CTRYAG 1 cut(s) 503
BisI GCNGC 5 cut(s) 90, 267, 364, 459, 503
BlsI GCNGC 5 cut(s) 91, 268, 365, 460, 504
Bme1390I CCNGG 2 cut(s) 255, 285
Bme18I GGWCC 2 cut(s) 21, 257
BmgBI CACGTC 1 cut(s) 227
BmgT120I GGNCC 3 cut(s) 21, 237, 257
BmiI GGNNCC 1 cut(s) 13
BmrFI CCNGG 2 cut(s) 255, 285
BpmI CTGGAG 1 cut(s) 237
Bpu10I CCTNAGC 1 cut(s) 85
BsaHI GRCGYC 1 cut(s) 80
BsaJI CCNNGG 2 cut(s) 146, 534
BsaXI ACNNNNNCTCC 2 cut(s) 102, 132
Bsc4I CCNNNNNNNGG 1 cut(s) 317
Bse1I ACTGG 2 cut(s) 212, 516
Bse21I CCTNAGG 1 cut(s) 17
BseBI CCWGG 2 cut(s) 255, 285
BseDI CCNNGG 2 cut(s) 146, 534
BseLI CCNNNNNNNGG 1 cut(s) 317
BseNI ACTGG 2 cut(s) 212, 516
BseRI GAGGAG 1 cut(s) 346
BseSI GKGCMC 1 cut(s) 107
BseXI GCAGC 4 cut(s) 76, 253, 445, 489
BsgI GTGCAG 1 cut(s) 428
Bsh1236I CGCG 2 cut(s) 43, 366
BshFI GGCC 1 cut(s) 239
BslI CCNNNNNNNGG 1 cut(s) 317
BsmAI GTCTC 1 cut(s) 166
BsmI GAATGC 1 cut(s) 368
BsnI GGCC 1 cut(s) 239
Bsp1286I GDGCHC 2 cut(s) 16, 107
Bsp19I CCATGG 2 cut(s) 146, 534
Bsp68I TCGCGA 1 cut(s) 43
BspACI CCGC 2 cut(s) 269, 364
BspANI GGCC 1 cut(s) 239
BspFNI CGCG 2 cut(s) 43, 366
BspLI GGNNCC 1 cut(s) 13
BspMAI CTGCAG 1 cut(s) 507
BsrI ACTGG 2 cut(s) 212, 516
BssECI CCNNGG 2 cut(s) 146, 534
BssNI GRCGYC 1 cut(s) 80
BssT1I CCWWGG 2 cut(s) 146, 534
Bst2UI CCWGG 2 cut(s) 255, 285
Bst6I CTCTTC 2 cut(s) 323, 513
BstACI GRCGYC 1 cut(s) 80
BstC8I GCNNGC 2 cut(s) 94, 241
BstDEI CTNAG 3 cut(s) 17, 85, 540
BstDSI CCRYGG 2 cut(s) 146, 534
BstFNI CGCG 2 cut(s) 43, 366
BstHHI GCGC 2 cut(s) 351, 368
BstMAI GTCTC 1 cut(s) 166
BstMWI GCNNNNNNNGC 3 cut(s) 102, 355, 464
BstNI CCWGG 2 cut(s) 255, 285
BstSCI CCNGG 2 cut(s) 253, 283
BstSFI CTRYAG 1 cut(s) 503
BstSLI GKGCMC 1 cut(s) 107
BstUI CGCG 2 cut(s) 43, 366
BstV1I GCAGC 4 cut(s) 76, 253, 445, 489
Bsu36I CCTNAGG 1 cut(s) 17
BsuRI GGCC 1 cut(s) 239
BtgI CCRYGG 2 cut(s) 146, 534
BtrI CACGTC 1 cut(s) 227
BtuMI TCGCGA 1 cut(s) 43
Cac8I GCNNGC 2 cut(s) 94, 241
CaiI CAGNNNCTG 1 cut(s) 511
CfoI GCGC 2 cut(s) 351, 368
Cfr13I GGNCC 3 cut(s) 21, 237, 257
CviAII CATG 4 cut(s) 76, 147, 352, 535
DdeI CTNAG 3 cut(s) 17, 85, 540
Eam1104I CTCTTC 2 cut(s) 323, 513
EarI CTCTTC 2 cut(s) 323, 513
Eco130I CCWWGG 2 cut(s) 146, 534
Eco24I GRGCYC 1 cut(s) 16
Eco47I GGWCC 2 cut(s) 21, 257
Eco81I CCTNAGG 1 cut(s) 17
EcoRII CCWGG 2 cut(s) 253, 283
EcoT14I CCWWGG 2 cut(s) 146, 534
EcoT38I GRGCYC 1 cut(s) 16
ErhI CCWWGG 2 cut(s) 146, 534
FaeI CATG 4 cut(s) 79, 150, 355, 538
FaiI YATR 8 cut(s) 50, 77, 148, 279, 353, 374, 434, 536
FatI CATG 4 cut(s) 75, 146, 351, 534
FblI GTMKAC 2 cut(s) 208, 216
Fnu4HI GCNGC 5 cut(s) 90, 267, 364, 459, 503
FriOI GRGCYC 1 cut(s) 16
Fsp4HI GCNGC 5 cut(s) 90, 267, 364, 459, 503
GlaI GCGC 2 cut(s) 350, 367
GluI GCNGC 5 cut(s) 90, 267, 364, 459, 503
GsuI CTGGAG 1 cut(s) 237
HaeIII GGCC 1 cut(s) 239
HhaI GCGC 2 cut(s) 351, 368
Hin1I GRCGYC 1 cut(s) 80
Hin1II CATG 4 cut(s) 79, 150, 355, 538
Hin6I GCGC 2 cut(s) 349, 366
HinP1I GCGC 2 cut(s) 349, 366
HincII GTYRAC 1 cut(s) 209
HindII GTYRAC 1 cut(s) 209
HinfI GANTC 3 cut(s) 6, 484, 492
HphI GGTGA 1 cut(s) 299
Hpy166II GTNNAC 3 cut(s) 209, 217, 483
Hpy188I TCNGA 1 cut(s) 11
Hpy188III TCNNGA 2 cut(s) 42, 57
Hpy8I GTNNAC 3 cut(s) 209, 217, 483
Hpy99I CGWCG 2 cut(s) 210, 231
HpyAV CCTTC 2 cut(s) 133, 311
HpyCH4IV ACGT 3 cut(s) 80, 205, 226
HpyCH4V TGCA 3 cut(s) 409, 467, 505
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 355, 464
HpyF3I CTNAG 3 cut(s) 17, 85, 540
HpySE526I ACGT 3 cut(s) 80, 205, 226
Hsp92I GRCGYC 1 cut(s) 80
Hsp92II CATG 4 cut(s) 79, 150, 355, 538
HspAI GCGC 2 cut(s) 349, 366
LmnI GCTCC 1 cut(s) 11
Lsp1109I GCAGC 4 cut(s) 76, 253, 445, 489
MaeII ACGT 3 cut(s) 80, 205, 226
MboII GAAGA 6 cut(s) 340, 355, 436, 527, 530, 535
MhlI GDGCHC 2 cut(s) 16, 107
MluCI AATT 1 cut(s) 529
MlyI GAGTC 2 cut(s) 478, 486
MmeI TCCRAC 1 cut(s) 189
MnlI CCTC 7 cut(s) 45, 73, 82, 261, 324, 327, 348
MspA1I CMGCKG 1 cut(s) 96
MspR9I CCNGG 2 cut(s) 255, 285
Mva1269I GAATGC 1 cut(s) 368
MvaI CCWGG 2 cut(s) 255, 285
MvnI CGCG 2 cut(s) 43, 366
MwoI GCNNNNNNNGC 3 cut(s) 102, 355, 464
NcoI CCATGG 2 cut(s) 146, 534
NlaIII CATG 4 cut(s) 79, 150, 355, 538
NlaIV GGNNCC 1 cut(s) 13
NmeAIII GCCGAG 1 cut(s) 523
NruI TCGCGA 1 cut(s) 43
PctI GAATGC 1 cut(s) 368
PfeI GAWTC 1 cut(s) 6
PfoI TCCNGGA 1 cut(s) 253
PkrI GCNGC 5 cut(s) 91, 268, 365, 460, 504
PleI GAGTC 2 cut(s) 478, 486
PpsI GAGTC 2 cut(s) 478, 486
PsiI TTATAA 1 cut(s) 434
Psp6I CCWGG 2 cut(s) 253, 283
PspGI CCWGG 2 cut(s) 253, 283
PspN4I GGNNCC 1 cut(s) 13
PspPI GGNCC 3 cut(s) 21, 237, 257
PstI CTGCAG 1 cut(s) 507
PstNI CAGNNNCTG 1 cut(s) 511
PvuII CAGCTG 1 cut(s) 96
RruI TCGCGA 1 cut(s) 43
SalI GTCGAC 1 cut(s) 207
SatI GCNGC 5 cut(s) 90, 267, 364, 459, 503
Sau96I GGNCC 3 cut(s) 21, 237, 257
SchI GAGTC 2 cut(s) 478, 486
ScrFI CCNGG 2 cut(s) 255, 285
SduI GDGCHC 2 cut(s) 16, 107
SfcI CTRYAG 1 cut(s) 503
SinI GGWCC 2 cut(s) 21, 257
Sse9I AATT 1 cut(s) 529
SsiI CCGC 2 cut(s) 269, 364
StyD4I CCNGG 2 cut(s) 253, 283
StyI CCWWGG 2 cut(s) 146, 534
TaiI ACGT 3 cut(s) 83, 208, 229
TaqI TCGA 2 cut(s) 208, 229
TasI AATT 1 cut(s) 529
TauI GCSGC 1 cut(s) 366
TfiI GAWTC 1 cut(s) 6
TseI GCWGC 4 cut(s) 89, 266, 458, 502
TspDTI ATGAA 3 cut(s) 115, 330, 361
VpaK11BI GGWCC 2 cut(s) 21, 257
XapI RAATTY 1 cut(s) 529
XmiI GTMKAC 2 cut(s) 208, 216
ZraI GACGTC 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.