FvH4_4g16760

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014233
Physical Location & Seq
Forward (+)
20608764 .. 20611016
2253 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g16760.t1

Sequence Viewer

Length: 1023 bp
ATGCTGGCGAGGGAATCGAGTTCGAACTTCGACCTTCCCGAGGAAGTGCTGCAAGTTCTGCCGCCCGATCCTTTCGAGCAGCTCGATGTGGCTCGCAAGATCACCTCCCTCGCCCTCTCGATACGTGTCTCCGCCCTCGAGTCCGACTGCGACGCCCTCCGCTCCCAGCTCGCCGACAAGGACCTCCTCATCGCCGACCTCCGCTCCCAGCTCCACTCCCTCGACTCCTCCCTCTCCGACACCGCCGACAAGCTCGCCCTCGCCGAGCAACAAAAGGAGGAGTTGGTGAAGGAGAAGAACTACCTGTCCAACACTGTGAGGAAGCTCAACAGAGATGTCTCCAAGCTGGAGGTGTTCAGGAAGACGCTGATGCGTTCGCTGCAGGAGGAGGACGACACCGGAGCTGCTGCTTCCGAAGTGGTTGCTAAGCCGACGGAGCTATCCGATGATAGCTTATCTTCTGCACCTCCAGAGCATGATGCTACATTACCACCGCCTTCGAGGACATCTTCAATCCAGAGCCAGGTCTCTGAAACCGGAAGCACATATACTGATGATCGTGACACAAATGCCACGAGGGCTCCTCGCATATCTCCGGGGCTCCTGTTAGCGTCACAAACTAGCACACCGCGGCTTACTCCTCCTGGTTCTCCTCCTGTCTATTCTGCATCCACATCCCCAACGAGGACTTCAAAGCCTGTGTCCCCAAGGCGGCATTCCATTTCATTTGCTACCTCAAGAGGCATGTTTGATGATAGATCTTCAGCACCATCAAGCCATCACGGTTCAATGTCAGGCTCTGAATATGGAAGGACTCGAGTGGATGGGAAAGAATTTTTCCGCCAAGTCAGGAGTCGTTTGTCTTATGAGCAGTTTGGTGCATTTCTGGCAAATGTTAAAGAACTAAATGCCCACAAGCAAACAAAAGAGGAGACTCTGCATAAAGCTGATGAGATATTTGGCCCAGATAATAAGGATCTATATGCCATATTTGAAGGATTGATCAGTCGCAATATCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

341

Amino Acids

37.45

Weight (kDa)

5.59

Isoelectric Point (pI)

56.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At4g15545_C PF25972 273 - 338 1.9e-34 At4g15545-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014233)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15545
fragaria_vesca FvH4_4g16760
malus_domestica MD13G1176600.v1.1 MD16G1178400.v1.1
prunus_persica Prupe.1G145800_v2.0.a1 Prupe.1G145800_v2.0.a1
pyrus_communis pycom13g15240 pycom16g14990
rosa_chinensis RchiOBHm_Chr4g0420331
rosa_laevigata RLG00000007772
rosa_roxburghii Rroxscaffold_5G00362980
rosa_samantha Rh4AG224500 Rh4BG227500 Rh4CG239000 Rh4DG223000
rosa_wichuraiana Rw0G005760 Rw4G019380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 162, 204
AccII CGCG 1 cut(s) 631
AclWI GGATC 2 cut(s) 62, 984
AcsI RAATTY 1 cut(s) 833
AcuI CTGAAG 1 cut(s) 747
AcyI GRCGYC 1 cut(s) 153
AfiI CCNNNNNNNGG 4 cut(s) 40, 523, 684, 711
AflIII ACRYGT 1 cut(s) 124
AgsI TTSAA 4 cut(s) 513, 693, 789, 995
AjnI CCWGG 2 cut(s) 522, 643
AjuI GAANNNNNNNTTGG 4 cut(s) 673, 700, 705, 732
AloI GAACNNNNNNTCC 2 cut(s) 290, 322
Alw26I GTCTC 4 cut(s) 133, 343, 532, 926
AlwI GGATC 2 cut(s) 62, 984
AlwNI CAGNNNCTG 1 cut(s) 800
Ama87I CYCGRG 3 cut(s) 38, 137, 816
AoxI GGCC 1 cut(s) 961
ApeKI GCWGC 5 cut(s) 49, 79, 379, 404, 407
ApoI RAATTY 1 cut(s) 833
ArsI GACNNNNNNTTYG 2 cut(s) 686, 718
AspS9I GGNCC 2 cut(s) 181, 962
AsuC2I CCSGG 1 cut(s) 597
AsuHPI GGTGA 2 cut(s) 94, 298
AsuII TTCGAA 1 cut(s) 23
AvaI CYCGRG 3 cut(s) 38, 137, 816
AvaII GGWCC 1 cut(s) 181
BanII GRGCYC 2 cut(s) 583, 603
BarI GAAGNNNNNNTAC 2 cut(s) 532, 564
BauI CACGAG 1 cut(s) 574
BbsI GAAGAC 1 cut(s) 368
BbvI GCAGC 5 cut(s) 36, 91, 366, 391, 394
BccI CCATC 3 cut(s) 778, 786, 818
BcgI CGANNNNNNTGC 2 cut(s) 404, 438
BciT130I CCWGG 2 cut(s) 524, 645
BclI TGATCA 1 cut(s) 1002
BcnI CCSGG 1 cut(s) 597
BcoDI GTCTC 4 cut(s) 133, 343, 532, 926
BfaI CTAG 1 cut(s) 621
BfmI CTRYAG 1 cut(s) 380
BglI GCCNNNNNGGC 1 cut(s) 578
BglII AGATCT 1 cut(s) 758
BisI GCNGC 8 cut(s) 50, 62, 80, 380, 405, 408, 632, 713
BlpI GCTNAGC 1 cut(s) 426
BlsI GCNGC 8 cut(s) 51, 63, 81, 381, 406, 409, 633, 714
Bme1390I CCNGG 3 cut(s) 524, 597, 645
Bme18I GGWCC 1 cut(s) 181
BmeT110I CYCGRG 3 cut(s) 38, 137, 816
BmgT120I GGNCC 2 cut(s) 181, 962
BmiI GGNNCC 2 cut(s) 582, 602
BmrFI CCNGG 3 cut(s) 524, 597, 645
BmsI GCATC 3 cut(s) 360, 469, 677
BpiI GAAGAC 1 cut(s) 368
BplI GAGNNNNNCTC 2 cut(s) 568, 600
BpmI CTGGAG 2 cut(s) 368, 453
Bpu1102I GCTNAGC 1 cut(s) 426
Bpu14I TTCGAA 1 cut(s) 23
BpuEI CTTGAG 1 cut(s) 721
BpuMI CCSGG 1 cut(s) 597
BsaAI YACGTR 1 cut(s) 125
BsaHI GRCGYC 1 cut(s) 153
BsaI GGTCTC 1 cut(s) 532
BsaJI CCNNGG 4 cut(s) 39, 596, 629, 707
BsaWI WCCGGW 2 cut(s) 398, 536
BsaXI ACNNNNNCTCC 4 cut(s) 188, 218, 565, 595
Bsc4I CCNNNNNNNGG 4 cut(s) 40, 523, 684, 711
BseBI CCWGG 2 cut(s) 524, 645
BseDI CCNNGG 4 cut(s) 39, 596, 629, 707
BseGI GGATG 3 cut(s) 668, 674, 829
BseLI CCNNNNNNNGG 4 cut(s) 40, 523, 684, 711
BseRI GAGGAG 8 cut(s) 176, 217, 293, 401, 573, 630, 642, 944
BseXI GCAGC 5 cut(s) 36, 91, 366, 391, 394
BseYI CCCAGC 2 cut(s) 165, 207
BsgI GTGCAG 1 cut(s) 447
Bsh1236I CGCG 1 cut(s) 631
BshFI GGCC 1 cut(s) 963
BsiHKCI CYCGRG 3 cut(s) 38, 137, 816
BsiSI CCGG 3 cut(s) 399, 537, 596
BslFI GGGAC 1 cut(s) 688
BslI CCNNNNNNNGG 4 cut(s) 40, 523, 684, 711
BsmAI GTCTC 4 cut(s) 133, 343, 532, 926
BsmFI GGGAC 1 cut(s) 688
BsmI GAATGC 1 cut(s) 715
BsnI GGCC 1 cut(s) 963
Bso31I GGTCTC 1 cut(s) 532
BsoBI CYCGRG 3 cut(s) 38, 137, 816
Bsp119I TTCGAA 1 cut(s) 23
Bsp1286I GDGCHC 2 cut(s) 583, 603
Bsp143I GATC 6 cut(s) 67, 99, 556, 758, 976, 1002
Bsp1720I GCTNAGC 1 cut(s) 426
BspANI GGCC 1 cut(s) 963
BspFNI CGCG 1 cut(s) 631
BspLI GGNNCC 2 cut(s) 582, 602
BspMAI CTGCAG 1 cut(s) 384
BspPI GGATC 2 cut(s) 62, 984
BspT104I TTCGAA 1 cut(s) 23
BspTNI GGTCTC 1 cut(s) 532
BsrBI CCGCTC 2 cut(s) 162, 204
BssECI CCNNGG 4 cut(s) 39, 596, 629, 707
BssMI GATC 6 cut(s) 67, 99, 556, 758, 976, 1002
BssNI GRCGYC 1 cut(s) 153
BssSI CACGAG 1 cut(s) 574
BssT1I CCWWGG 1 cut(s) 707
Bst2BI CACGAG 1 cut(s) 574
Bst2UI CCWGG 2 cut(s) 524, 645
Bst4CI ACNGT 2 cut(s) 316, 785
BstACI GRCGYC 1 cut(s) 153
BstAPI GCANNNNNTGC 1 cut(s) 58
BstBAI YACGTR 1 cut(s) 125
BstBI TTCGAA 1 cut(s) 23
BstC8I GCNNGC 4 cut(s) 6, 94, 171, 255
BstDEI CTNAG 1 cut(s) 426
BstDSI CCRYGG 1 cut(s) 629
BstENI CCTNNNNNAGG 1 cut(s) 38
BstF5I GGATG 3 cut(s) 668, 674, 829
BstFNI CGCG 1 cut(s) 631
BstKTI GATC 6 cut(s) 70, 102, 559, 761, 979, 1005
BstMAI GTCTC 4 cut(s) 133, 343, 532, 926
BstMBI GATC 6 cut(s) 67, 99, 556, 758, 976, 1002
BstMWI GCNNNNNNNGC 5 cut(s) 58, 379, 436, 578, 887
BstNI CCWGG 2 cut(s) 524, 645
BstNSI RCATGY 1 cut(s) 748
BstSCI CCNGG 3 cut(s) 522, 595, 643
BstSFI CTRYAG 1 cut(s) 380
BstUI CGCG 1 cut(s) 631
BstV1I GCAGC 5 cut(s) 36, 91, 366, 391, 394
BstV2I GAAGAC 1 cut(s) 368
BstX2I RGATCY 2 cut(s) 758, 976
BstYI RGATCY 2 cut(s) 758, 976
BsuRI GGCC 1 cut(s) 963
BtgI CCRYGG 1 cut(s) 629
BtgZI GCGATG 1 cut(s) 175
BtsCI GGATG 3 cut(s) 668, 674, 829
BtsIMutI CAGTG 1 cut(s) 312
Cac8I GCNNGC 4 cut(s) 6, 94, 171, 255
CaiI CAGNNNCTG 1 cut(s) 800
Cfr13I GGNCC 2 cut(s) 181, 962
Cfr42I CCGCGG 1 cut(s) 632
CseI GACGC 3 cut(s) 161, 373, 600
CviAII CATG 2 cut(s) 476, 745
DdeI CTNAG 1 cut(s) 426
DpnI GATC 6 cut(s) 69, 101, 558, 760, 978, 1004
DpnII GATC 6 cut(s) 67, 99, 556, 758, 976, 1002
EciI GGCGGA 2 cut(s) 121, 830
Eco130I CCWWGG 1 cut(s) 707
Eco24I GRGCYC 2 cut(s) 583, 603
Eco31I GGTCTC 1 cut(s) 532
Eco47I GGWCC 1 cut(s) 181
Eco57I CTGAAG 1 cut(s) 747
Eco88I CYCGRG 3 cut(s) 38, 137, 816
EcoNI CCTNNNNNAGG 1 cut(s) 38
EcoO109I RGGNCCY 1 cut(s) 181
EcoRII CCWGG 2 cut(s) 522, 643
EcoT14I CCWWGG 1 cut(s) 707
EcoT38I GRGCYC 2 cut(s) 583, 603
ErhI CCWWGG 1 cut(s) 707
FaeI CATG 2 cut(s) 479, 748
FaqI GGGAC 1 cut(s) 688
FatI CATG 2 cut(s) 475, 744
FbaI TGATCA 1 cut(s) 1002
Fnu4HI GCNGC 8 cut(s) 50, 62, 80, 380, 405, 408, 632, 713
FokI GGATG 3 cut(s) 655, 661, 836
FriOI GRGCYC 2 cut(s) 583, 603
Fsp4HI GCNGC 8 cut(s) 50, 62, 80, 380, 405, 408, 632, 713
FspBI CTAG 1 cut(s) 621
GluI GCNGC 8 cut(s) 50, 62, 80, 380, 405, 408, 632, 713
GsaI CCCAGC 2 cut(s) 169, 211
GsuI CTGGAG 2 cut(s) 368, 453
HaeIII GGCC 1 cut(s) 963
HapII CCGG 3 cut(s) 399, 537, 596
HgaI GACGC 3 cut(s) 161, 373, 600
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 2 cut(s) 479, 748
HinfI GANTC 6 cut(s) 14, 140, 224, 814, 853, 934
HpaII CCGG 3 cut(s) 399, 537, 596
HphI GGTGA 2 cut(s) 94, 298
Hpy188I TCNGA 6 cut(s) 145, 238, 415, 445, 532, 802
Hpy188III TCNNGA 8 cut(s) 38, 118, 358, 470, 517, 560, 738, 850
Hpy99I CGWCG 2 cut(s) 155, 436
HpyAV CCTTC 5 cut(s) 44, 283, 507, 804, 989
HpyCH4III ACNGT 2 cut(s) 316, 785
HpyCH4IV ACGT 1 cut(s) 124
HpyCH4V TGCA 6 cut(s) 52, 382, 464, 668, 881, 940
HpyF10VI GCNNNNNNNGC 5 cut(s) 58, 379, 436, 578, 887
HpyF3I CTNAG 1 cut(s) 426
HpySE526I ACGT 1 cut(s) 124
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 2 cut(s) 479, 748
Ksp22I TGATCA 1 cut(s) 1002
KspI CCGCGG 1 cut(s) 632
Kzo9I GATC 6 cut(s) 67, 99, 556, 758, 976, 1002
LmnI GCTCC 7 cut(s) 167, 209, 216, 401, 436, 586, 606
Lsp1109I GCAGC 5 cut(s) 36, 91, 366, 391, 394
LweI GCATC 3 cut(s) 360, 469, 677
MaeI CTAG 1 cut(s) 621
MaeII ACGT 1 cut(s) 124
MaeIII GTNAC 2 cut(s) 560, 612
MalI GATC 6 cut(s) 69, 101, 558, 760, 978, 1004
MbiI CCGCTC 2 cut(s) 162, 204
MboI GATC 6 cut(s) 67, 99, 556, 758, 976, 1002
MboII GAAGA 5 cut(s) 307, 373, 450, 501, 753
MflI RGATCY 2 cut(s) 758, 976
MhlI GDGCHC 2 cut(s) 583, 603
MluCI AATT 1 cut(s) 833
MlyI GAGTC 5 cut(s) 149, 218, 808, 862, 928
MmeI TCCRAC 3 cut(s) 168, 261, 333
MseI TTAA 1 cut(s) 897
MspA1I CMGCKG 1 cut(s) 631
MspI CCGG 3 cut(s) 399, 537, 596
MspR9I CCNGG 3 cut(s) 524, 597, 645
Mva1269I GAATGC 1 cut(s) 715
MvaI CCWGG 2 cut(s) 524, 645
MvnI CGCG 1 cut(s) 631
MwoI GCNNNNNNNGC 5 cut(s) 58, 379, 436, 578, 887
NciI CCSGG 1 cut(s) 597
NdeII GATC 6 cut(s) 67, 99, 556, 758, 976, 1002
NlaIII CATG 2 cut(s) 479, 748
NlaIV GGNNCC 2 cut(s) 582, 602
NmeAIII GCCGAG 1 cut(s) 289
NmuCI GTSAC 2 cut(s) 560, 612
NspI RCATGY 1 cut(s) 748
NspV TTCGAA 1 cut(s) 23
PaeR7I CTCGAG 2 cut(s) 137, 816
PcsI WCGNNNNNNNCGW 3 cut(s) 36, 81, 261
PctI GAATGC 1 cut(s) 715
PfeI GAWTC 1 cut(s) 14
PkrI GCNGC 8 cut(s) 51, 63, 81, 381, 406, 409, 633, 714
PleI GAGTC 5 cut(s) 148, 218, 808, 861, 928
PpsI GAGTC 5 cut(s) 148, 218, 808, 861, 928
Ppu21I YACGTR 1 cut(s) 125
PpuMI RGGWCCY 1 cut(s) 181
Psp5II RGGWCCY 1 cut(s) 181
Psp6I CCWGG 2 cut(s) 522, 643
PspFI CCCAGC 2 cut(s) 165, 207
PspGI CCWGG 2 cut(s) 522, 643
PspN4I GGNNCC 2 cut(s) 582, 602
PspPI GGNCC 2 cut(s) 181, 962
PspPPI RGGWCCY 1 cut(s) 181
PspXI VCTCGAGB 2 cut(s) 137, 816
PstI CTGCAG 1 cut(s) 384
PstNI CAGNNNCTG 1 cut(s) 800
PsuI RGATCY 2 cut(s) 758, 976
SacII CCGCGG 1 cut(s) 632
SaqAI TTAA 1 cut(s) 897
SatI GCNGC 8 cut(s) 50, 62, 80, 380, 405, 408, 632, 713
Sau3AI GATC 6 cut(s) 67, 99, 556, 758, 976, 1002
Sau96I GGNCC 2 cut(s) 181, 962
SchI GAGTC 5 cut(s) 149, 218, 808, 862, 928
ScrFI CCNGG 3 cut(s) 524, 597, 645
SduI GDGCHC 2 cut(s) 583, 603
SfaNI GCATC 3 cut(s) 360, 469, 677
SfcI CTRYAG 1 cut(s) 380
Sfr274I CTCGAG 2 cut(s) 137, 816
Sfr303I CCGCGG 1 cut(s) 632
SfuI TTCGAA 1 cut(s) 23
SgrBI CCGCGG 1 cut(s) 632
SinI GGWCC 1 cut(s) 181
SlaI CTCGAG 2 cut(s) 137, 816
SmlI CTYRAG 3 cut(s) 137, 736, 816
SmoI CTYRAG 3 cut(s) 137, 736, 816
Sse9I AATT 1 cut(s) 833
SspMI CTAG 1 cut(s) 621
StyD4I CCNGG 3 cut(s) 522, 595, 643
StyI CCWWGG 1 cut(s) 707
TaaI ACNGT 2 cut(s) 316, 785
TaiI ACGT 1 cut(s) 127
TasI AATT 1 cut(s) 833
TauI GCSGC 3 cut(s) 64, 634, 715
TfiI GAWTC 1 cut(s) 14
Tru1I TTAA 1 cut(s) 897
Tru9I TTAA 1 cut(s) 897
TscAI CASTG 1 cut(s) 319
TseFI GTSAC 2 cut(s) 560, 612
TseI GCWGC 5 cut(s) 49, 79, 379, 404, 407
Tsp45I GTSAC 2 cut(s) 560, 612
TspDTI ATGAA 1 cut(s) 714
TspGWI ACGGA 1 cut(s) 449
TspRI CASTG 1 cut(s) 319
VpaK11BI GGWCC 1 cut(s) 181
XagI CCTNNNNNAGG 1 cut(s) 38
XapI RAATTY 1 cut(s) 833
XceI RCATGY 1 cut(s) 748
XhoI CTCGAG 2 cut(s) 137, 816
XspI CTAG 1 cut(s) 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.