Rh4DG223000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014233
Physical Location & Seq
Forward (+)
42627204 .. 42630755
3552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG223000.1

Sequence Viewer

Length: 861 bp
ATGCTGGCGAGGGAATCGAGCAGTTGGAACTTCGATCTTCCCGAGGAAGTGCTCCAAGTTCTGCCGCCCGATCCTTTCGAGCAGCTCGATGTGGCCCGCAAGATCACCTCCATTGCCCTCGCGACACGTGTCTCCTCCCTCGAGTCCGAGGCCGACGCCCTTCGCGCCCAGCTCGCCGACAAGGACCGCCTCGTCGCCGACCTCCAGGCCCAGGTCCAGTCCCTCGACGCCTCCCTCTCCGACACCGCCGACAAGCTCGCCCTCGCCGAGCAGGAAAAGGAGGAGTTGTTGAAGGAGAAGAGCTACCTGTCCAATACTGTGAGGAAGCTCAACAGAGATGTCTCCAAGTTGGAGGTGTTCAGAAAGACGCTTATGCGTTCGCTGCAGGAGCAGGACGAGAGTGGAGCTTCGGAGGTGGTTGCTAAGGCGGAGGAGCAGACCGGAGAGAGCTTATCTTCTCAATCGCAGACTGGACCTGCAGAACATGATGCCACATTGCCACCTTCGAAAACATCTTCAATCCAGAGCCAATTCTCTGAAACAGGAAGCACATATACTGAGGATCGTGACACAAATGCCTCGAGGGCTCCTCGCATATCACCGGGGCTCCTATTAGCATCCCAAACCAGCACACCGCGGCTTACTCCCCCGGGTTCTCCTCCTATCTATTCGGCATCCACATCCCCAACTAGGACATCAAAGCCGGTGTCCCCAAGGCGGCATTCCATGTCATTTACTACCTCAAGAGGCATGTTTGATGATAGATCTTCGGTGCCCTCAAGCCATCACGGCTCCATGCCAGGCGCTGAACATGGAAGGACTCGAGTGGATGGGAAAGAATTTTTCCGCCAAGTCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

286

Amino Acids

31.32

Weight (kDa)

5.53

Isoelectric Point (pI)

55.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014233)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15545
fragaria_vesca FvH4_4g16760
malus_domestica MD13G1176600.v1.1 MD16G1178400.v1.1
prunus_persica Prupe.1G145800_v2.0.a1 Prupe.1G145800_v2.0.a1
pyrus_communis pycom13g15240 pycom16g14990
rosa_chinensis RchiOBHm_Chr4g0420331
rosa_laevigata RLG00000007772
rosa_roxburghii Rroxscaffold_5G00362980
rosa_samantha Rh4AG224500 Rh4BG227500 Rh4CG239000 Rh4DG223000
rosa_wichuraiana Rw0G005760 Rw4G019380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 191
AbsI CCTCGAGG 1 cut(s) 580
Acc36I ACCTGC 1 cut(s) 484
AccB1I GGYRCC 1 cut(s) 772
AccII CGCG 3 cut(s) 122, 165, 637
AciI CCGC 9 cut(s) 65, 97, 187, 246, 428, 635, 637, 718, 847
AclWI GGATC 2 cut(s) 65, 570
AcsI RAATTY 1 cut(s) 839
AcvI CACGTG 1 cut(s) 128
AcyI GRCGYC 2 cut(s) 156, 228
AfiI CCNNNNNNNGG 3 cut(s) 211, 690, 717
AflIII ACRYGT 2 cut(s) 125, 127
AgsI TTSAA 2 cut(s) 292, 519
AjnI CCWGG 3 cut(s) 204, 210, 799
AjuI GAANNNNNNNTTGG 2 cut(s) 706, 738
AluBI AGCT 7 cut(s) 85, 172, 256, 303, 328, 407, 450
AluI AGCT 7 cut(s) 85, 172, 256, 303, 328, 407, 450
Alw21I GWGCWC 1 cut(s) 54
Alw26I GTCTC 2 cut(s) 136, 346
AlwI GGATC 2 cut(s) 65, 570
AlwNI CAGNNNCTG 1 cut(s) 806
Ama87I CYCGRG 5 cut(s) 41, 140, 580, 649, 822
AoxI GGCC 3 cut(s) 93, 150, 207
ApeKI GCWGC 2 cut(s) 82, 382
ApoI RAATTY 1 cut(s) 839
ArsI GACNNNNNNTTYG 2 cut(s) 692, 724
AspLEI GCGC 2 cut(s) 167, 806
AspS9I GGNCC 5 cut(s) 94, 184, 208, 214, 473
AsuC2I CCSGG 3 cut(s) 603, 650, 651
AsuHPI GGTGA 2 cut(s) 97, 591
AsuII TTCGAA 1 cut(s) 506
AvaI CYCGRG 5 cut(s) 41, 140, 580, 649, 822
AvaII GGWCC 3 cut(s) 184, 214, 473
BaeGI GKGCMC 1 cut(s) 777
BanI GGYRCC 1 cut(s) 772
BanII GRGCYC 2 cut(s) 589, 609
BarI GAAGNNNNNNTAC 2 cut(s) 538, 570
BbrPI CACGTG 1 cut(s) 128
Bbv12I GWGCWC 1 cut(s) 54
BbvI GCAGC 2 cut(s) 94, 369
BccI CCATC 2 cut(s) 792, 824
BceAI ACGGC 1 cut(s) 805
BciT130I CCWGG 3 cut(s) 206, 212, 801
BcnI CCSGG 3 cut(s) 603, 650, 651
BcoDI GTCTC 2 cut(s) 136, 346
BfaI CTAG 1 cut(s) 690
BfmI CTRYAG 2 cut(s) 383, 477
BfoI RGCGCY 1 cut(s) 807
BfuAI ACCTGC 1 cut(s) 484
BglI GCCNNNNNGGC 2 cut(s) 584, 789
BglII AGATCT 1 cut(s) 764
BisI GCNGC 5 cut(s) 65, 83, 383, 638, 719
BlsI GCNGC 5 cut(s) 66, 84, 384, 639, 720
Bme1390I CCNGG 6 cut(s) 206, 212, 603, 650, 651, 801
Bme18I GGWCC 3 cut(s) 184, 214, 473
BmeT110I CYCGRG 5 cut(s) 41, 140, 580, 649, 822
BmgT120I GGNCC 5 cut(s) 94, 184, 208, 214, 473
BmiI GGNNCC 4 cut(s) 588, 608, 774, 793
BmrFI CCNGG 6 cut(s) 206, 212, 603, 650, 651, 801
BmsI GCATC 3 cut(s) 478, 626, 683
BoxI GACNNNNGTC 1 cut(s) 128
BplI GAGNNNNNCTC 2 cut(s) 574, 606
BpmI CTGGAG 1 cut(s) 188
Bpu10I CCTNAGC 1 cut(s) 423
Bpu14I TTCGAA 1 cut(s) 506
BpuEI CTTGAG 2 cut(s) 727, 763
BpuMI CCSGG 3 cut(s) 603, 650, 651
BsaAI YACGTR 1 cut(s) 128
BsaHI GRCGYC 2 cut(s) 156, 228
BsaJI CCNNGG 8 cut(s) 42, 147, 210, 602, 635, 648, 649, 713
BsaWI WCCGGW 1 cut(s) 440
BsaXI ACNNNNNCTCC 4 cut(s) 272, 302, 591, 621
Bsc4I CCNNNNNNNGG 3 cut(s) 211, 690, 717
Bse118I RCCGGY 1 cut(s) 703
Bse1I ACTGG 2 cut(s) 217, 475
Bse3DI GCAATG 2 cut(s) 111, 494
BseBI CCWGG 3 cut(s) 206, 212, 801
BseDI CCNNGG 8 cut(s) 42, 147, 210, 602, 635, 648, 649, 713
BseGI GGATG 4 cut(s) 617, 674, 680, 835
BseLI CCNNNNNNNGG 3 cut(s) 211, 690, 717
BseMI GCAATG 2 cut(s) 111, 494
BseMII CTCAG 1 cut(s) 549
BseNI ACTGG 2 cut(s) 217, 475
BseRI GAGGAG 5 cut(s) 124, 296, 446, 579, 648
BseSI GKGCMC 1 cut(s) 777
BseXI GCAGC 2 cut(s) 94, 369
BseYI CCCAGC 1 cut(s) 168
Bsh1236I CGCG 3 cut(s) 122, 165, 637
BshFI GGCC 3 cut(s) 95, 152, 209
BshNI GGYRCC 1 cut(s) 772
BsiHKAI GWGCWC 1 cut(s) 54
BsiHKCI CYCGRG 5 cut(s) 41, 140, 580, 649, 822
BsiSI CCGG 4 cut(s) 441, 602, 650, 704
BslFI GGGAC 2 cut(s) 205, 694
BslI CCNNNNNNNGG 3 cut(s) 211, 690, 717
BsmAI GTCTC 2 cut(s) 136, 346
BsmFI GGGAC 2 cut(s) 205, 694
BsmI GAATGC 1 cut(s) 721
BsnI GGCC 3 cut(s) 95, 152, 209
BsoBI CYCGRG 5 cut(s) 41, 140, 580, 649, 822
Bsp119I TTCGAA 1 cut(s) 506
Bsp1286I GDGCHC 4 cut(s) 54, 589, 609, 777
Bsp143I GATC 5 cut(s) 34, 70, 102, 562, 764
Bsp68I TCGCGA 1 cut(s) 122
BspACI CCGC 9 cut(s) 65, 97, 187, 246, 428, 635, 637, 718, 847
BspANI GGCC 3 cut(s) 95, 152, 209
BspCNI CTCAG 1 cut(s) 550
BspFNI CGCG 3 cut(s) 122, 165, 637
BspLI GGNNCC 4 cut(s) 588, 608, 774, 793
BspMAI CTGCAG 2 cut(s) 387, 481
BspMI ACCTGC 1 cut(s) 484
BspPI GGATC 2 cut(s) 65, 570
BspQI GCTCTTC 1 cut(s) 293
BspT104I TTCGAA 1 cut(s) 506
BspT107I GGYRCC 1 cut(s) 772
BsrDI GCAATG 2 cut(s) 111, 494
BsrFI RCCGGY 1 cut(s) 703
BsrI ACTGG 2 cut(s) 217, 475
BssAI RCCGGY 1 cut(s) 703
BssECI CCNNGG 8 cut(s) 42, 147, 210, 602, 635, 648, 649, 713
BssMI GATC 5 cut(s) 34, 70, 102, 562, 764
BssNI GRCGYC 2 cut(s) 156, 228
BssT1I CCWWGG 1 cut(s) 713
Bst2UI CCWGG 3 cut(s) 206, 212, 801
Bst4CI ACNGT 1 cut(s) 319
Bst6I CTCTTC 1 cut(s) 293
BstACI GRCGYC 2 cut(s) 156, 228
BstBAI YACGTR 1 cut(s) 128
BstBI TTCGAA 1 cut(s) 506
BstC8I GCNNGC 4 cut(s) 6, 97, 174, 258
BstDEI CTNAG 2 cut(s) 423, 558
BstDSI CCRYGG 1 cut(s) 635
BstF5I GGATG 4 cut(s) 617, 674, 680, 835
BstFNI CGCG 3 cut(s) 122, 165, 637
BstH2I RGCGCY 1 cut(s) 807
BstHHI GCGC 2 cut(s) 167, 806
BstKTI GATC 5 cut(s) 37, 73, 105, 565, 767
BstMAI GTCTC 2 cut(s) 136, 346
BstMBI GATC 5 cut(s) 34, 70, 102, 562, 764
BstMWI GCNNNNNNNGC 6 cut(s) 164, 173, 382, 388, 584, 789
BstNI CCWGG 3 cut(s) 206, 212, 801
BstNSI RCATGY 1 cut(s) 754
BstPAI GACNNNNGTC 1 cut(s) 128
BstSCI CCNGG 6 cut(s) 204, 210, 601, 648, 649, 799
BstSFI CTRYAG 2 cut(s) 383, 477
BstSLI GKGCMC 1 cut(s) 777
BstUI CGCG 3 cut(s) 122, 165, 637
BstV1I GCAGC 2 cut(s) 94, 369
BstX2I RGATCY 1 cut(s) 764
BstYI RGATCY 1 cut(s) 764
BsuRI GGCC 3 cut(s) 95, 152, 209
BtgI CCRYGG 1 cut(s) 635
BtsCI GGATG 4 cut(s) 617, 674, 680, 835
BtuMI TCGCGA 1 cut(s) 122
BveI ACCTGC 1 cut(s) 484
Cac8I GCNNGC 4 cut(s) 6, 97, 174, 258
CaiI CAGNNNCTG 1 cut(s) 806
CfoI GCGC 2 cut(s) 167, 806
Cfr10I RCCGGY 1 cut(s) 703
Cfr13I GGNCC 5 cut(s) 94, 184, 208, 214, 473
Cfr42I CCGCGG 1 cut(s) 638
Cfr9I CCCGGG 1 cut(s) 649
CseI GACGC 3 cut(s) 164, 236, 376
CviAII CATG 5 cut(s) 485, 727, 751, 796, 812
DdeI CTNAG 2 cut(s) 423, 558
DpnI GATC 5 cut(s) 36, 72, 104, 564, 766
DpnII GATC 5 cut(s) 34, 70, 102, 562, 764
DrdI GACNNNNNNGTC 1 cut(s) 191
DseDI GACNNNNNNGTC 1 cut(s) 191
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
EciI GGCGGA 2 cut(s) 443, 836
Eco130I CCWWGG 1 cut(s) 713
Eco24I GRGCYC 2 cut(s) 589, 609
Eco47I GGWCC 3 cut(s) 184, 214, 473
Eco72I CACGTG 1 cut(s) 128
Eco88I CYCGRG 5 cut(s) 41, 140, 580, 649, 822
EcoRII CCWGG 3 cut(s) 204, 210, 799
EcoT14I CCWWGG 1 cut(s) 713
EcoT38I GRGCYC 2 cut(s) 589, 609
ErhI CCWWGG 1 cut(s) 713
FaeI CATG 5 cut(s) 488, 730, 754, 799, 815
FaiI YATR 9 cut(s) 374, 486, 553, 555, 596, 728, 752, 797, 813
FaqI GGGAC 2 cut(s) 205, 694
FatI CATG 5 cut(s) 484, 726, 750, 795, 811
FauI CCCGC 1 cut(s) 104
Fnu4HI GCNGC 5 cut(s) 65, 83, 383, 638, 719
FokI GGATG 4 cut(s) 604, 661, 667, 842
FriOI GRGCYC 2 cut(s) 589, 609
Fsp4HI GCNGC 5 cut(s) 65, 83, 383, 638, 719
FspBI CTAG 1 cut(s) 690
GlaI GCGC 2 cut(s) 166, 805
GluI GCNGC 5 cut(s) 65, 83, 383, 638, 719
GsaI CCCAGC 1 cut(s) 172
GsuI CTGGAG 1 cut(s) 188
HaeII RGCGCY 1 cut(s) 807
HaeIII GGCC 3 cut(s) 95, 152, 209
HapII CCGG 4 cut(s) 441, 602, 650, 704
HgaI GACGC 3 cut(s) 164, 236, 376
HhaI GCGC 2 cut(s) 167, 806
Hin1I GRCGYC 2 cut(s) 156, 228
Hin1II CATG 5 cut(s) 488, 730, 754, 799, 815
Hin6I GCGC 2 cut(s) 165, 804
HinP1I GCGC 2 cut(s) 165, 804
HinfI GANTC 3 cut(s) 14, 143, 820
HpaII CCGG 4 cut(s) 441, 602, 650, 704
HphI GGTGA 2 cut(s) 97, 591
Hpy188I TCNGA 5 cut(s) 148, 241, 362, 412, 538
Hpy188III TCNNGA 5 cut(s) 41, 121, 523, 566, 744
Hpy99I CGWCG 3 cut(s) 158, 197, 230
HpyAV CCTTC 4 cut(s) 170, 286, 513, 810
HpyCH4III ACNGT 1 cut(s) 319
HpyCH4IV ACGT 1 cut(s) 127
HpyCH4V TGCA 2 cut(s) 385, 479
HpyF10VI GCNNNNNNNGC 6 cut(s) 164, 173, 382, 388, 584, 789
HpyF3I CTNAG 2 cut(s) 423, 558
HpySE526I ACGT 1 cut(s) 127
Hsp92I GRCGYC 2 cut(s) 156, 228
Hsp92II CATG 5 cut(s) 488, 730, 754, 799, 815
HspAI GCGC 2 cut(s) 165, 804
KspI CCGCGG 1 cut(s) 638
Kzo9I GATC 5 cut(s) 34, 70, 102, 562, 764
LguI GCTCTTC 1 cut(s) 293
LmnI GCTCC 7 cut(s) 57, 388, 404, 433, 592, 612, 797
Lsp1109I GCAGC 2 cut(s) 94, 369
LweI GCATC 3 cut(s) 478, 626, 683
MaeI CTAG 1 cut(s) 690
MaeII ACGT 1 cut(s) 127
MaeIII GTNAC 1 cut(s) 566
MalI GATC 5 cut(s) 36, 72, 104, 564, 766
MboI GATC 5 cut(s) 34, 70, 102, 562, 764
MboII GAAGA 5 cut(s) 29, 310, 447, 507, 759
MflI RGATCY 1 cut(s) 764
MhlI GDGCHC 4 cut(s) 54, 589, 609, 777
MluCI AATT 2 cut(s) 530, 839
MlyI GAGTC 2 cut(s) 152, 814
MmeI TCCRAC 3 cut(s) 5, 264, 330
MspA1I CMGCKG 1 cut(s) 637
MspI CCGG 4 cut(s) 441, 602, 650, 704
MspR9I CCNGG 6 cut(s) 206, 212, 603, 650, 651, 801
Mva1269I GAATGC 1 cut(s) 721
MvaI CCWGG 3 cut(s) 206, 212, 801
MvnI CGCG 3 cut(s) 122, 165, 637
MwoI GCNNNNNNNGC 6 cut(s) 164, 173, 382, 388, 584, 789
NciI CCSGG 3 cut(s) 603, 650, 651
NdeII GATC 5 cut(s) 34, 70, 102, 562, 764
NlaIII CATG 5 cut(s) 488, 730, 754, 799, 815
NlaIV GGNNCC 4 cut(s) 588, 608, 774, 793
NmeAIII GCCGAG 1 cut(s) 292
NmuCI GTSAC 1 cut(s) 566
NruI TCGCGA 1 cut(s) 122
NspI RCATGY 1 cut(s) 754
NspV TTCGAA 1 cut(s) 506
PaeR7I CTCGAG 3 cut(s) 140, 580, 822
PciSI GCTCTTC 1 cut(s) 293
PcsI WCGNNNNNNNCGW 3 cut(s) 39, 84, 264
PctI GAATGC 1 cut(s) 721
PfeI GAWTC 1 cut(s) 14
PkrI GCNGC 5 cut(s) 66, 84, 384, 639, 720
PleI GAGTC 2 cut(s) 151, 814
PmaCI CACGTG 1 cut(s) 128
PmlI CACGTG 1 cut(s) 128
PpsI GAGTC 2 cut(s) 151, 814
Ppu21I YACGTR 1 cut(s) 128
PshAI GACNNNNGTC 1 cut(s) 128
Psp6I CCWGG 3 cut(s) 204, 210, 799
PspCI CACGTG 1 cut(s) 128
PspFI CCCAGC 1 cut(s) 168
PspGI CCWGG 3 cut(s) 204, 210, 799
PspN4I GGNNCC 4 cut(s) 588, 608, 774, 793
PspPI GGNCC 5 cut(s) 94, 184, 208, 214, 473
PspXI VCTCGAGB 3 cut(s) 140, 580, 822
PstI CTGCAG 2 cut(s) 387, 481
PstNI CAGNNNCTG 1 cut(s) 806
PsuI RGATCY 1 cut(s) 764
RruI TCGCGA 1 cut(s) 122
SacII CCGCGG 1 cut(s) 638
SapI GCTCTTC 1 cut(s) 293
SatI GCNGC 5 cut(s) 65, 83, 383, 638, 719
Sau3AI GATC 5 cut(s) 34, 70, 102, 562, 764
Sau96I GGNCC 5 cut(s) 94, 184, 208, 214, 473
SchI GAGTC 2 cut(s) 152, 814
ScrFI CCNGG 6 cut(s) 206, 212, 603, 650, 651, 801
SduI GDGCHC 4 cut(s) 54, 589, 609, 777
SfaNI GCATC 3 cut(s) 478, 626, 683
SfcI CTRYAG 2 cut(s) 383, 477
Sfr274I CTCGAG 3 cut(s) 140, 580, 822
Sfr303I CCGCGG 1 cut(s) 638
SfuI TTCGAA 1 cut(s) 506
SgrBI CCGCGG 1 cut(s) 638
SinI GGWCC 3 cut(s) 184, 214, 473
SlaI CTCGAG 3 cut(s) 140, 580, 822
SmaI CCCGGG 1 cut(s) 651
SmlI CTYRAG 5 cut(s) 140, 580, 742, 778, 822
SmoI CTYRAG 5 cut(s) 140, 580, 742, 778, 822
Sse9I AATT 2 cut(s) 530, 839
SsiI CCGC 9 cut(s) 65, 97, 187, 246, 428, 635, 637, 718, 847
SspMI CTAG 1 cut(s) 690
StyD4I CCNGG 6 cut(s) 204, 210, 601, 648, 649, 799
StyI CCWWGG 1 cut(s) 713
TaaI ACNGT 1 cut(s) 319
TaiI ACGT 1 cut(s) 130
TaqI TCGA 9 cut(s) 17, 33, 78, 87, 141, 225, 506, 581, 823
TasI AATT 2 cut(s) 530, 839
TauI GCSGC 3 cut(s) 67, 640, 721
TfiI GAWTC 1 cut(s) 14
TseFI GTSAC 1 cut(s) 566
TseI GCWGC 2 cut(s) 82, 382
Tsp45I GTSAC 1 cut(s) 566
TspMI CCCGGG 1 cut(s) 649
VpaK11BI GGWCC 3 cut(s) 184, 214, 473
XapI RAATTY 1 cut(s) 839
XceI RCATGY 1 cut(s) 754
XhoI CTCGAG 3 cut(s) 140, 580, 822
XmaI CCCGGG 1 cut(s) 649
XspI CTAG 1 cut(s) 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.