MD13G1176600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014233
Physical Location & Seq
Reverse (-)
14638382 .. 14641124
2743 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1176600.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGCTTGCGAAGGAATCGGGGAGTTCGAACTTCGATCTTCCCGAGGAAGTGTTGGAAGTACTGCCGCCGGATCCTTTCGAGCAGCTCGATGTGGCCCGCAAAATCACGTCCCTTGCGCTCTCGACACGTGTCTCGGCCCTGGAATCAGAGTCGTCCGCTCTGCGCGAGAAGCTCGCCGAGAAGGACCGGCTCATAGCCGACCTCCAATCACAGGTGGAGTCCCTGGACGCCTCTCTCTCCGAATCCGCTGACAAGCTCGCCCTCGCGAACCAGGAGAAGGAGGGATTGGTGAAGGAGAAGGCCGTACTCGCCAACACTGTGAGGAAGCTGAGCAGAGATGTCTCGAAGCTGGAGGTGTTCAGAAAGACCCTTATGCGATCACTTGCAGAGGATGAAGAAAATCCTAGTGGAGCTTCAGACGTTGTTGCTAAGCCTGCTAATACTGTACAAGCCGAAGAGCACTCGTCTTCAAAACCACATGATGGAGATGGAGAAGGTGATGTCACATTTTCCACATTACCACCGTCGAGGTCATCTTCAGTCCAAAGCACGTTTTCTGGAGGAAGTACGTTTACCGAGGATCGCGACACAGATGCCTCAAGACCTCGCATAGCACACAGCCTCTTGCTAGCATCCCAAACTAGCACACCCCGGTTCACTCCCCCAGGTTCTCCTCCTATCTATTCTGCATCCACATCACCAACAAGAACATCGAAACCTGGGTCTCCAAAGCGACATTCCATGTCCTTCGCCACCTCAAGAGGCATGTTTGACAACAGATCATCTGTGCCCTCAAGCCATCACGGCTCTGAATCTGGAAGAACTAGGGTGGATGGGAAAGAATTTTTCCGCCAAGTCAGGAGTCGTTTGTCTTACGAGCAGTTTGGTGCATTCCTGGCAAATGTTAAGGAACTAAACGGCCACAAGCAAACCAAAGAGGAGACATTACAGAAGGCAGATGAGATATTTGGGCCAGATAACAAGGACCTATATGCTATTTTTGAAGGATTAATCAGTCGCAATATCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

344

Amino Acids

37.41

Weight (kDa)

5.73

Isoelectric Point (pI)

54.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At4g15545_C PF25972 276 - 341 9e-34 At4g15545-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014233)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15545
fragaria_vesca FvH4_4g16760
malus_domestica MD13G1176600.v1.1 MD16G1178400.v1.1
prunus_persica Prupe.1G145800_v2.0.a1 Prupe.1G145800_v2.0.a1
pyrus_communis pycom13g15240 pycom16g14990
rosa_chinensis RchiOBHm_Chr4g0420331
rosa_laevigata RLG00000007772
rosa_roxburghii Rroxscaffold_5G00362980
rosa_samantha Rh4AG224500 Rh4BG227500 Rh4CG239000 Rh4DG223000
rosa_wichuraiana Rw0G005760 Rw4G019380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 482
AccBSI CCGCTC 1 cut(s) 158
AccII CGCG 3 cut(s) 165, 266, 585
AciI CCGC 5 cut(s) 65, 97, 156, 246, 850
AclWI GGATC 3 cut(s) 65, 78, 588
AcoI YGGCCR 1 cut(s) 919
AcsI RAATTY 1 cut(s) 842
AcuI CTGAAG 2 cut(s) 399, 522
AcvI CACGTG 1 cut(s) 128
AcyI GRCGYC 1 cut(s) 228
AfaI GTAC 4 cut(s) 60, 306, 447, 568
AfiI CCNNNNNNNGG 3 cut(s) 211, 277, 482
AflIII ACRYGT 2 cut(s) 125, 127
AgsI TTSAA 2 cut(s) 471, 1004
AjiI CACGTC 1 cut(s) 108
AjnI CCWGG 6 cut(s) 138, 222, 270, 664, 718, 894
AjuI GAANNNNNNNTTGG 4 cut(s) 269, 301, 721, 753
AluBI AGCT 6 cut(s) 85, 172, 256, 328, 349, 413
AluI AGCT 6 cut(s) 85, 172, 256, 328, 349, 413
Alw21I GWGCWC 1 cut(s) 462
Alw26I GTCTC 4 cut(s) 136, 346, 729, 935
AlwI GGATC 3 cut(s) 65, 78, 588
Ama87I CYCGRG 1 cut(s) 41
AoxI GGCC 5 cut(s) 93, 135, 300, 919, 971
ApeKI GCWGC 1 cut(s) 82
ApoI RAATTY 1 cut(s) 842
ArsI GACNNNNNNTTYG 2 cut(s) 707, 739
AseI ATTAAT 1 cut(s) 1010
AspLEI GCGC 2 cut(s) 118, 165
AspS9I GGNCC 5 cut(s) 94, 136, 184, 971, 985
AsuC2I CCSGG 1 cut(s) 652
AsuHPI GGTGA 3 cut(s) 301, 509, 690
AsuII TTCGAA 1 cut(s) 26
AsuNHI GCTAGC 1 cut(s) 628
AvaI CYCGRG 1 cut(s) 41
AvaII GGWCC 2 cut(s) 184, 985
BaeGI GKGCMC 1 cut(s) 792
BamHI GGATCC 1 cut(s) 70
BarI GAAGNNNNNNTAC 2 cut(s) 556, 588
BbrPI CACGTG 1 cut(s) 128
BbsI GAAGAC 1 cut(s) 459
Bbv12I GWGCWC 1 cut(s) 462
BbvI GCAGC 1 cut(s) 94
BccI CCATC 4 cut(s) 476, 482, 807, 827
BceAI ACGGC 3 cut(s) 287, 820, 934
BcgI CGANNNNNNTGC 2 cut(s) 575, 609
BciT130I CCWGG 6 cut(s) 140, 224, 272, 666, 720, 896
BcnI CCSGG 1 cut(s) 652
BcoDI GTCTC 4 cut(s) 136, 346, 729, 935
BfaI CTAG 4 cut(s) 405, 629, 642, 825
BglI GCCNNNNNGGC 1 cut(s) 804
BisI GCNGC 2 cut(s) 65, 83
BlpI GCTNAGC 2 cut(s) 329, 429
BlsI GCNGC 2 cut(s) 66, 84
BmcAI AGTACT 1 cut(s) 60
Bme1390I CCNGG 7 cut(s) 140, 224, 272, 652, 666, 720, 896
Bme18I GGWCC 2 cut(s) 184, 985
BmeT110I CYCGRG 1 cut(s) 41
BmgBI CACGTC 1 cut(s) 108
BmgT120I GGNCC 5 cut(s) 94, 136, 184, 971, 985
BmiI GGNNCC 1 cut(s) 72
BmrFI CCNGG 7 cut(s) 140, 224, 272, 652, 666, 720, 896
BmsI GCATC 3 cut(s) 583, 641, 698
BmtI GCTAGC 1 cut(s) 632
BoxI GACNNNNGTC 1 cut(s) 128
BpiI GAAGAC 1 cut(s) 459
BpmI CTGGAG 2 cut(s) 371, 579
Bpu1102I GCTNAGC 2 cut(s) 329, 429
Bpu14I TTCGAA 1 cut(s) 26
BpuEI CTTGAG 3 cut(s) 583, 742, 778
BpuMI CCSGG 1 cut(s) 652
BsaAI YACGTR 1 cut(s) 128
BsaHI GRCGYC 1 cut(s) 228
BsaI GGTCTC 1 cut(s) 729
BsaJI CCNNGG 7 cut(s) 42, 138, 222, 576, 650, 664, 719
Bsc4I CCNNNNNNNGG 3 cut(s) 211, 277, 482
Bse118I RCCGGY 1 cut(s) 186
BseBI CCWGG 6 cut(s) 140, 224, 272, 666, 720, 896
BseDI CCNNGG 7 cut(s) 42, 138, 222, 576, 650, 664, 719
BseGI GGATG 4 cut(s) 397, 632, 689, 838
BseLI CCNNNNNNNGG 3 cut(s) 211, 277, 482
BseMII CTCAG 1 cut(s) 320
BseRI GAGGAG 2 cut(s) 663, 953
BseSI GKGCMC 1 cut(s) 792
BseXI GCAGC 1 cut(s) 94
Bsh1236I CGCG 3 cut(s) 165, 266, 585
BshFI GGCC 5 cut(s) 95, 137, 302, 921, 973
BsiHKAI GWGCWC 1 cut(s) 462
BsiHKCI CYCGRG 1 cut(s) 41
BsiSI CCGG 3 cut(s) 68, 187, 652
BslFI GGGAC 2 cut(s) 94, 205
BslI CCNNNNNNNGG 3 cut(s) 211, 277, 482
BsmAI GTCTC 4 cut(s) 136, 346, 729, 935
BsmFI GGGAC 2 cut(s) 94, 205
BsmI GAATGC 1 cut(s) 890
BsnI GGCC 5 cut(s) 95, 137, 302, 921, 973
Bso31I GGTCTC 1 cut(s) 729
BsoBI CYCGRG 1 cut(s) 41
Bsp119I TTCGAA 1 cut(s) 26
Bsp1286I GDGCHC 2 cut(s) 462, 792
Bsp1407I TGTACA 1 cut(s) 445
Bsp143I GATC 5 cut(s) 34, 70, 377, 580, 779
Bsp1720I GCTNAGC 2 cut(s) 329, 429
Bsp68I TCGCGA 2 cut(s) 266, 585
BspACI CCGC 5 cut(s) 65, 97, 156, 246, 850
BspANI GGCC 5 cut(s) 95, 137, 302, 921, 973
BspCNI CTCAG 1 cut(s) 321
BspFNI CGCG 3 cut(s) 165, 266, 585
BspLI GGNNCC 1 cut(s) 72
BspOI GCTAGC 1 cut(s) 632
BspPI GGATC 3 cut(s) 65, 78, 588
BspQI GCTCTTC 1 cut(s) 450
BspT104I TTCGAA 1 cut(s) 26
BspTNI GGTCTC 1 cut(s) 729
BsrBI CCGCTC 1 cut(s) 158
BsrFI RCCGGY 1 cut(s) 186
BsrGI TGTACA 1 cut(s) 445
BssAI RCCGGY 1 cut(s) 186
BssECI CCNNGG 7 cut(s) 42, 138, 222, 576, 650, 664, 719
BssMI GATC 5 cut(s) 34, 70, 377, 580, 779
BssNI GRCGYC 1 cut(s) 228
Bst2UI CCWGG 6 cut(s) 140, 224, 272, 666, 720, 896
Bst4CI ACNGT 3 cut(s) 319, 445, 525
Bst6I CTCTTC 1 cut(s) 450
BstACI GRCGYC 1 cut(s) 228
BstAUI TGTACA 1 cut(s) 445
BstBAI YACGTR 1 cut(s) 128
BstBI TTCGAA 1 cut(s) 26
BstC8I GCNNGC 6 cut(s) 6, 97, 174, 258, 435, 630
BstDEI CTNAG 2 cut(s) 329, 429
BstF5I GGATG 4 cut(s) 397, 632, 689, 838
BstFNI CGCG 3 cut(s) 165, 266, 585
BstHHI GCGC 2 cut(s) 118, 165
BstKTI GATC 5 cut(s) 37, 73, 380, 583, 782
BstMAI GTCTC 4 cut(s) 136, 346, 729, 935
BstMBI GATC 5 cut(s) 34, 70, 377, 580, 779
BstMWI GCNNNNNNNGC 5 cut(s) 169, 308, 434, 804, 896
BstNI CCWGG 6 cut(s) 140, 224, 272, 666, 720, 896
BstNSI RCATGY 1 cut(s) 769
BstPAI GACNNNNGTC 1 cut(s) 128
BstSCI CCNGG 7 cut(s) 138, 222, 270, 650, 664, 718, 894
BstSLI GKGCMC 1 cut(s) 792
BstUI CGCG 3 cut(s) 165, 266, 585
BstV1I GCAGC 1 cut(s) 94
BstV2I GAAGAC 1 cut(s) 459
BstX2I RGATCY 1 cut(s) 70
BstYI RGATCY 1 cut(s) 70
BsuRI GGCC 5 cut(s) 95, 137, 302, 921, 973
BtrI CACGTC 1 cut(s) 108
BtsCI GGATG 4 cut(s) 397, 632, 689, 838
BtsIMutI CAGTG 1 cut(s) 315
BtuMI TCGCGA 2 cut(s) 266, 585
Cac8I GCNNGC 6 cut(s) 6, 97, 174, 258, 435, 630
CfoI GCGC 2 cut(s) 118, 165
Cfr10I RCCGGY 1 cut(s) 186
Cfr13I GGNCC 5 cut(s) 94, 136, 184, 971, 985
CseI GACGC 1 cut(s) 236
Csp6I GTAC 4 cut(s) 59, 305, 446, 567
CviAII CATG 3 cut(s) 479, 742, 766
CviQI GTAC 4 cut(s) 59, 305, 446, 567
DdeI CTNAG 2 cut(s) 329, 429
DpnI GATC 5 cut(s) 36, 72, 379, 582, 781
DpnII GATC 5 cut(s) 34, 70, 377, 580, 779
EaeI YGGCCR 1 cut(s) 919
Eam1104I CTCTTC 1 cut(s) 450
EarI CTCTTC 1 cut(s) 450
EciI GGCGGA 1 cut(s) 839
Eco31I GGTCTC 1 cut(s) 729
Eco47I GGWCC 2 cut(s) 184, 985
Eco57I CTGAAG 2 cut(s) 399, 522
Eco72I CACGTG 1 cut(s) 128
Eco88I CYCGRG 1 cut(s) 41
EcoO109I RGGNCCY 1 cut(s) 985
EcoRII CCWGG 6 cut(s) 138, 222, 270, 664, 718, 894
FaeI CATG 3 cut(s) 482, 745, 769
FaiI YATR 8 cut(s) 194, 374, 480, 611, 743, 767, 991, 993
FaqI GGGAC 2 cut(s) 94, 205
FatI CATG 3 cut(s) 478, 741, 765
FauI CCCGC 1 cut(s) 104
Fnu4HI GCNGC 2 cut(s) 65, 83
FokI GGATG 4 cut(s) 404, 619, 676, 845
Fsp4HI GCNGC 2 cut(s) 65, 83
FspBI CTAG 4 cut(s) 405, 629, 642, 825
GlaI GCGC 2 cut(s) 117, 164
GluI GCNGC 2 cut(s) 65, 83
GsuI CTGGAG 2 cut(s) 371, 579
HaeIII GGCC 5 cut(s) 95, 137, 302, 921, 973
HapII CCGG 3 cut(s) 68, 187, 652
HgaI GACGC 1 cut(s) 236
HhaI GCGC 2 cut(s) 118, 165
Hin1I GRCGYC 1 cut(s) 228
Hin1II CATG 3 cut(s) 482, 745, 769
Hin6I GCGC 2 cut(s) 116, 163
HinP1I GCGC 2 cut(s) 116, 163
HinfI GANTC 7 cut(s) 14, 143, 149, 218, 242, 812, 862
HpaII CCGG 3 cut(s) 68, 187, 652
HphI GGTGA 3 cut(s) 301, 509, 690
Hpy166II GTNNAC 2 cut(s) 573, 657
Hpy188I TCNGA 5 cut(s) 148, 241, 362, 418, 811
Hpy8I GTNNAC 2 cut(s) 573, 657
Hpy99I CGWCG 1 cut(s) 529
HpyAV CCTTC 9 cut(s) 4, 175, 271, 286, 292, 488, 757, 946, 998
HpyCH4III ACNGT 3 cut(s) 319, 445, 525
HpyCH4IV ACGT 5 cut(s) 107, 127, 420, 551, 569
HpyCH4V TGCA 3 cut(s) 386, 689, 890
HpyF10VI GCNNNNNNNGC 5 cut(s) 169, 308, 434, 804, 896
HpyF3I CTNAG 2 cut(s) 329, 429
HpySE526I ACGT 5 cut(s) 107, 127, 420, 551, 569
Hsp92I GRCGYC 1 cut(s) 228
Hsp92II CATG 3 cut(s) 482, 745, 769
HspAI GCGC 2 cut(s) 116, 163
Kzo9I GATC 5 cut(s) 34, 70, 377, 580, 779
LguI GCTCTTC 1 cut(s) 450
LmnI GCTCC 1 cut(s) 410
Lsp1109I GCAGC 1 cut(s) 94
LweI GCATC 3 cut(s) 583, 641, 698
MaeI CTAG 4 cut(s) 405, 629, 642, 825
MaeII ACGT 5 cut(s) 107, 127, 420, 551, 569
MaeIII GTNAC 1 cut(s) 502
MalI GATC 5 cut(s) 36, 72, 379, 582, 781
MbiI CCGCTC 1 cut(s) 158
MboI GATC 5 cut(s) 34, 70, 377, 580, 779
MboII GAAGA 6 cut(s) 29, 407, 459, 467, 528, 831
MflI RGATCY 1 cut(s) 70
MhlI GDGCHC 2 cut(s) 462, 792
MluCI AATT 1 cut(s) 842
MlyI GAGTC 3 cut(s) 158, 227, 871
MmeI TCCRAC 1 cut(s) 33
MseI TTAA 3 cut(s) 906, 1010, 1030
MspA1I CMGCKG 1 cut(s) 248
MspI CCGG 3 cut(s) 68, 187, 652
MspR9I CCNGG 7 cut(s) 140, 224, 272, 652, 666, 720, 896
Mva1269I GAATGC 1 cut(s) 890
MvaI CCWGG 6 cut(s) 140, 224, 272, 666, 720, 896
MvnI CGCG 3 cut(s) 165, 266, 585
MwoI GCNNNNNNNGC 5 cut(s) 169, 308, 434, 804, 896
NciI CCSGG 1 cut(s) 652
NdeII GATC 5 cut(s) 34, 70, 377, 580, 779
NheI GCTAGC 1 cut(s) 628
NlaIII CATG 3 cut(s) 482, 745, 769
NlaIV GGNNCC 1 cut(s) 72
NmeAIII GCCGAG 2 cut(s) 113, 202
NmuCI GTSAC 1 cut(s) 502
NruI TCGCGA 2 cut(s) 266, 585
NspI RCATGY 1 cut(s) 769
NspV TTCGAA 1 cut(s) 26
PciSI GCTCTTC 1 cut(s) 450
PcsI WCGNNNNNNNCGW 3 cut(s) 23, 39, 84
PctI GAATGC 1 cut(s) 890
PfeI GAWTC 4 cut(s) 14, 143, 242, 812
PflMI CCANNNNNTGG 1 cut(s) 482
PkrI GCNGC 2 cut(s) 66, 84
PleI GAGTC 3 cut(s) 157, 226, 870
PmaCI CACGTG 1 cut(s) 128
PmlI CACGTG 1 cut(s) 128
PpsI GAGTC 3 cut(s) 157, 226, 870
Ppu21I YACGTR 1 cut(s) 128
PpuMI RGGWCCY 1 cut(s) 985
PshAI GACNNNNGTC 1 cut(s) 128
PshBI ATTAAT 1 cut(s) 1010
Psp5II RGGWCCY 1 cut(s) 985
Psp6I CCWGG 6 cut(s) 138, 222, 270, 664, 718, 894
PspCI CACGTG 1 cut(s) 128
PspGI CCWGG 6 cut(s) 138, 222, 270, 664, 718, 894
PspN4I GGNNCC 1 cut(s) 72
PspPI GGNCC 5 cut(s) 94, 136, 184, 971, 985
PspPPI RGGWCCY 1 cut(s) 985
PsuI RGATCY 1 cut(s) 70
RruI TCGCGA 2 cut(s) 266, 585
RsaI GTAC 4 cut(s) 60, 306, 447, 568
RsaNI GTAC 4 cut(s) 59, 305, 446, 567
SapI GCTCTTC 1 cut(s) 450
SaqAI TTAA 3 cut(s) 906, 1010, 1030
SatI GCNGC 2 cut(s) 65, 83
Sau3AI GATC 5 cut(s) 34, 70, 377, 580, 779
Sau96I GGNCC 5 cut(s) 94, 136, 184, 971, 985
ScaI AGTACT 1 cut(s) 60
SchI GAGTC 3 cut(s) 158, 227, 871
ScrFI CCNGG 7 cut(s) 140, 224, 272, 652, 666, 720, 896
SduI GDGCHC 2 cut(s) 462, 792
SfaNI GCATC 3 cut(s) 583, 641, 698
SfuI TTCGAA 1 cut(s) 26
SinI GGWCC 2 cut(s) 184, 985
SmlI CTYRAG 3 cut(s) 598, 757, 793
SmoI CTYRAG 3 cut(s) 598, 757, 793
Sse9I AATT 1 cut(s) 842
SsiI CCGC 5 cut(s) 65, 97, 156, 246, 850
SspMI CTAG 4 cut(s) 405, 629, 642, 825
StyD4I CCNGG 7 cut(s) 138, 222, 270, 650, 664, 718, 894
TaaI ACNGT 3 cut(s) 319, 445, 525
TaiI ACGT 5 cut(s) 110, 130, 423, 554, 572
TaqI TCGA 8 cut(s) 26, 33, 78, 87, 122, 344, 527, 713
TasI AATT 1 cut(s) 842
TatI WGTACW 2 cut(s) 58, 445
TauI GCSGC 1 cut(s) 67
TfiI GAWTC 4 cut(s) 14, 143, 242, 812
Tru1I TTAA 3 cut(s) 906, 1010, 1030
Tru9I TTAA 3 cut(s) 906, 1010, 1030
TscAI CASTG 1 cut(s) 322
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 1 cut(s) 82
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 1 cut(s) 408
TspRI CASTG 1 cut(s) 322
Van91I CCANNNNNTGG 1 cut(s) 482
VpaK11BI GGWCC 2 cut(s) 184, 985
VspI ATTAAT 1 cut(s) 1010
XapI RAATTY 1 cut(s) 842
XceI RCATGY 1 cut(s) 769
XspI CTAG 4 cut(s) 405, 629, 642, 825
ZrmI AGTACT 1 cut(s) 60
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.