Rw4G019380

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014233
Physical Location & Seq
Forward (+)
42621937 .. 42625082
3146 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G019380.1

Sequence Viewer

Length: 1029 bp
ATGCTGGCGAGGGAATCGAGCAGTTGGAACTTCGATCTTCCCGAGGAAGTGCTGCAAGTTCTGCCGCCCGATCCTTTCGAGCAGCTCGATGTGGCCCGCAAGATCACCTCCATTGCCCTCGCGACACGTGTCTCCTCCCTCGAGTCAGAGGCCGACGCCCTTCGCGCCCAGCTCGCCGACAAGGACCACCTCGTCGCCGACCTCCAGGCCCAGGTCCAGTCCCTCGACGCCTCTCTCTCCGACACCGCCGACAAGCTCGCCCTCGCCGAGCAGGAAAAGGAGGAGTTGTTGAAGGAGAAGAGCTACCTGTCCAATACTGTGAGGAAGCTGAACAGAGATGTCTCCAAGTTGGAGGTGTTCAGAAAGACGCTTATGCGTTCGCTGCAGGAGCAGGACGAGAGTGGAGCTTCGGAGGTGGTTGCTAAGGCGGAGGAGCAGACCGGAGAGAGCTTATCTTCTCAATCGCAGAGTGGGCCTGCAGAACATGATGCCACATTGCCACCTTCGAAAACATCTTCAATCCAGAGCCAATTCTCTGAAACAGGAAGCACATATACTGAGGATCGTGACACAAATGCCTCGAGGGCTCCTCGCATATCACCGGGGCTTCTATTAGCATCCCAAACCAGCACACCACGGCTTACTCCCCCGGGTTCTCCTCCTATCTATTCGGCATCCACATCCCCAACTAGGACATCAAAGCCGGTGTCCCCAAGGCGGCATTCCATGTCATTTACTACCTCAAGAGGCATGTTTGATGATAGATCTTCGGTGCCCTCAAGCCATCACGGCTCCATGCCAGGCGCTGAACATGGAAGGACTCGAGTGGATGGGAAAGAATTTTTCCGCCAAGTCAGGAGTCGTTTGTCTTATGAGCAGTTTGGTGCATTTCTGGCAAATGTTAAGGAACTAAATGCCCACAAGCAAACAAAAGAGGAGACTCTGCATAAAGCTGGTGAGATATTTGGCCCAGATAATAAGGATCTATATGTCATATTTGAAGGATTGATCAGTCGTAATGTCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

342

Amino Acids

37.67

Weight (kDa)

5.68

Isoelectric Point (pI)

55.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At4g15545_C PF25972 275 - 340 1.2e-33 At4g15545-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014233)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15545
fragaria_vesca FvH4_4g16760
malus_domestica MD13G1176600.v1.1 MD16G1178400.v1.1
prunus_persica Prupe.1G145800_v2.0.a1 Prupe.1G145800_v2.0.a1
pyrus_communis pycom13g15240 pycom16g14990
rosa_chinensis RchiOBHm_Chr4g0420331
rosa_laevigata RLG00000007772
rosa_roxburghii Rroxscaffold_5G00362980
rosa_samantha Rh4AG224500 Rh4BG227500 Rh4CG239000 Rh4DG223000
rosa_wichuraiana Rw0G005760 Rw4G019380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 191
AbsI CCTCGAGG 1 cut(s) 580
AccB1I GGYRCC 1 cut(s) 772
AccII CGCG 2 cut(s) 122, 165
AciI CCGC 6 cut(s) 65, 97, 246, 428, 718, 847
AclWI GGATC 3 cut(s) 65, 570, 990
AcsI RAATTY 1 cut(s) 839
AcvI CACGTG 1 cut(s) 128
AcyI GRCGYC 2 cut(s) 156, 228
AfiI CCNNNNNNNGG 3 cut(s) 211, 690, 717
AflIII ACRYGT 2 cut(s) 125, 127
AgsI TTSAA 3 cut(s) 292, 519, 1001
AjnI CCWGG 3 cut(s) 204, 210, 799
AjuI GAANNNNNNNTTGG 2 cut(s) 706, 738
AluBI AGCT 8 cut(s) 85, 172, 256, 303, 328, 407, 450, 953
AluI AGCT 8 cut(s) 85, 172, 256, 303, 328, 407, 450, 953
Alw26I GTCTC 3 cut(s) 136, 346, 932
AlwI GGATC 3 cut(s) 65, 570, 990
AlwNI CAGNNNCTG 1 cut(s) 806
Ama87I CYCGRG 5 cut(s) 41, 140, 580, 649, 822
AoxI GGCC 5 cut(s) 93, 150, 207, 473, 967
ApeKI GCWGC 3 cut(s) 52, 82, 382
ApoI RAATTY 1 cut(s) 839
ArsI GACNNNNNNTTYG 2 cut(s) 692, 724
AspLEI GCGC 2 cut(s) 167, 806
AspS9I GGNCC 6 cut(s) 94, 184, 208, 214, 473, 968
AsuC2I CCSGG 3 cut(s) 603, 650, 651
AsuHPI GGTGA 3 cut(s) 97, 591, 968
AsuII TTCGAA 1 cut(s) 506
AvaI CYCGRG 5 cut(s) 41, 140, 580, 649, 822
AvaII GGWCC 2 cut(s) 184, 214
BaeGI GKGCMC 1 cut(s) 777
BanI GGYRCC 1 cut(s) 772
BanII GRGCYC 1 cut(s) 589
BarI GAAGNNNNNNTAC 2 cut(s) 538, 570
BbrPI CACGTG 1 cut(s) 128
BbvI GCAGC 3 cut(s) 39, 94, 369
BccI CCATC 2 cut(s) 792, 824
BceAI ACGGC 2 cut(s) 653, 805
BciT130I CCWGG 3 cut(s) 206, 212, 801
BclI TGATCA 1 cut(s) 1008
BcnI CCSGG 3 cut(s) 603, 650, 651
BcoDI GTCTC 3 cut(s) 136, 346, 932
BfaI CTAG 1 cut(s) 690
BfmI CTRYAG 2 cut(s) 383, 477
BfoI RGCGCY 1 cut(s) 807
BglI GCCNNNNNGGC 2 cut(s) 584, 789
BglII AGATCT 1 cut(s) 764
BisI GCNGC 5 cut(s) 53, 65, 83, 383, 719
BlsI GCNGC 5 cut(s) 54, 66, 84, 384, 720
Bme1390I CCNGG 6 cut(s) 206, 212, 603, 650, 651, 801
Bme18I GGWCC 2 cut(s) 184, 214
BmeT110I CYCGRG 5 cut(s) 41, 140, 580, 649, 822
BmgT120I GGNCC 6 cut(s) 94, 184, 208, 214, 473, 968
BmiI GGNNCC 3 cut(s) 588, 774, 793
BmrFI CCNGG 6 cut(s) 206, 212, 603, 650, 651, 801
BmsI GCATC 3 cut(s) 478, 626, 683
BoxI GACNNNNGTC 1 cut(s) 128
BplI GAGNNNNNCTC 2 cut(s) 574, 606
BpmI CTGGAG 1 cut(s) 188
Bpu10I CCTNAGC 1 cut(s) 423
Bpu14I TTCGAA 1 cut(s) 506
BpuEI CTTGAG 2 cut(s) 727, 763
BpuMI CCSGG 3 cut(s) 603, 650, 651
BsaAI YACGTR 1 cut(s) 128
BsaHI GRCGYC 2 cut(s) 156, 228
BsaJI CCNNGG 7 cut(s) 42, 210, 602, 635, 648, 649, 713
BsaWI WCCGGW 1 cut(s) 440
BsaXI ACNNNNNCTCC 2 cut(s) 272, 302
Bsc4I CCNNNNNNNGG 3 cut(s) 211, 690, 717
Bse118I RCCGGY 1 cut(s) 703
Bse1I ACTGG 1 cut(s) 217
Bse3DI GCAATG 2 cut(s) 111, 494
BseBI CCWGG 3 cut(s) 206, 212, 801
BseDI CCNNGG 7 cut(s) 42, 210, 602, 635, 648, 649, 713
BseGI GGATG 4 cut(s) 617, 674, 680, 835
BseLI CCNNNNNNNGG 3 cut(s) 211, 690, 717
BseMI GCAATG 2 cut(s) 111, 494
BseMII CTCAG 1 cut(s) 549
BseNI ACTGG 1 cut(s) 217
BseRI GAGGAG 6 cut(s) 124, 296, 446, 579, 648, 950
BseSI GKGCMC 1 cut(s) 777
BseXI GCAGC 3 cut(s) 39, 94, 369
BseYI CCCAGC 1 cut(s) 168
Bsh1236I CGCG 2 cut(s) 122, 165
BshFI GGCC 5 cut(s) 95, 152, 209, 475, 969
BshNI GGYRCC 1 cut(s) 772
BsiHKCI CYCGRG 5 cut(s) 41, 140, 580, 649, 822
BsiSI CCGG 4 cut(s) 441, 602, 650, 704
BslFI GGGAC 2 cut(s) 205, 694
BslI CCNNNNNNNGG 3 cut(s) 211, 690, 717
BsmAI GTCTC 3 cut(s) 136, 346, 932
BsmFI GGGAC 2 cut(s) 205, 694
BsmI GAATGC 1 cut(s) 721
BsnI GGCC 5 cut(s) 95, 152, 209, 475, 969
BsoBI CYCGRG 5 cut(s) 41, 140, 580, 649, 822
Bsp119I TTCGAA 1 cut(s) 506
Bsp1286I GDGCHC 2 cut(s) 589, 777
Bsp143I GATC 7 cut(s) 34, 70, 102, 562, 764, 982, 1008
Bsp68I TCGCGA 1 cut(s) 122
BspACI CCGC 6 cut(s) 65, 97, 246, 428, 718, 847
BspANI GGCC 5 cut(s) 95, 152, 209, 475, 969
BspCNI CTCAG 1 cut(s) 550
BspFNI CGCG 2 cut(s) 122, 165
BspLI GGNNCC 3 cut(s) 588, 774, 793
BspMAI CTGCAG 2 cut(s) 387, 481
BspPI GGATC 3 cut(s) 65, 570, 990
BspQI GCTCTTC 1 cut(s) 293
BspT104I TTCGAA 1 cut(s) 506
BspT107I GGYRCC 1 cut(s) 772
BsrDI GCAATG 2 cut(s) 111, 494
BsrFI RCCGGY 1 cut(s) 703
BsrI ACTGG 1 cut(s) 217
BssAI RCCGGY 1 cut(s) 703
BssECI CCNNGG 7 cut(s) 42, 210, 602, 635, 648, 649, 713
BssMI GATC 7 cut(s) 34, 70, 102, 562, 764, 982, 1008
BssNI GRCGYC 2 cut(s) 156, 228
BssT1I CCWWGG 1 cut(s) 713
Bst2UI CCWGG 3 cut(s) 206, 212, 801
Bst4CI ACNGT 1 cut(s) 319
Bst6I CTCTTC 1 cut(s) 293
BstACI GRCGYC 2 cut(s) 156, 228
BstAPI GCANNNNNTGC 1 cut(s) 61
BstBAI YACGTR 1 cut(s) 128
BstBI TTCGAA 1 cut(s) 506
BstC8I GCNNGC 5 cut(s) 6, 97, 174, 258, 477
BstDEI CTNAG 2 cut(s) 423, 558
BstDSI CCRYGG 1 cut(s) 635
BstF5I GGATG 4 cut(s) 617, 674, 680, 835
BstFNI CGCG 2 cut(s) 122, 165
BstH2I RGCGCY 1 cut(s) 807
BstHHI GCGC 2 cut(s) 167, 806
BstKTI GATC 7 cut(s) 37, 73, 105, 565, 767, 985, 1011
BstMAI GTCTC 3 cut(s) 136, 346, 932
BstMBI GATC 7 cut(s) 34, 70, 102, 562, 764, 982, 1008
BstMWI GCNNNNNNNGC 9 cut(s) 61, 164, 173, 382, 388, 472, 584, 789, 893
BstNI CCWGG 3 cut(s) 206, 212, 801
BstNSI RCATGY 1 cut(s) 754
BstPAI GACNNNNGTC 1 cut(s) 128
BstSCI CCNGG 6 cut(s) 204, 210, 601, 648, 649, 799
BstSFI CTRYAG 2 cut(s) 383, 477
BstSLI GKGCMC 1 cut(s) 777
BstUI CGCG 2 cut(s) 122, 165
BstV1I GCAGC 3 cut(s) 39, 94, 369
BstX2I RGATCY 2 cut(s) 764, 982
BstYI RGATCY 2 cut(s) 764, 982
BsuRI GGCC 5 cut(s) 95, 152, 209, 475, 969
BtgI CCRYGG 1 cut(s) 635
BtsCI GGATG 4 cut(s) 617, 674, 680, 835
BtuMI TCGCGA 1 cut(s) 122
Cac8I GCNNGC 5 cut(s) 6, 97, 174, 258, 477
CaiI CAGNNNCTG 1 cut(s) 806
CfoI GCGC 2 cut(s) 167, 806
Cfr10I RCCGGY 1 cut(s) 703
Cfr13I GGNCC 6 cut(s) 94, 184, 208, 214, 473, 968
Cfr9I CCCGGG 1 cut(s) 649
CseI GACGC 3 cut(s) 164, 236, 376
CviAII CATG 5 cut(s) 485, 727, 751, 796, 812
DdeI CTNAG 2 cut(s) 423, 558
DpnI GATC 7 cut(s) 36, 72, 104, 564, 766, 984, 1010
DpnII GATC 7 cut(s) 34, 70, 102, 562, 764, 982, 1008
DrdI GACNNNNNNGTC 1 cut(s) 191
DseDI GACNNNNNNGTC 1 cut(s) 191
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
EciI GGCGGA 2 cut(s) 443, 836
Eco130I CCWWGG 1 cut(s) 713
Eco24I GRGCYC 1 cut(s) 589
Eco47I GGWCC 2 cut(s) 184, 214
Eco72I CACGTG 1 cut(s) 128
Eco88I CYCGRG 5 cut(s) 41, 140, 580, 649, 822
EcoRII CCWGG 3 cut(s) 204, 210, 799
EcoT14I CCWWGG 1 cut(s) 713
EcoT38I GRGCYC 1 cut(s) 589
ErhI CCWWGG 1 cut(s) 713
FaeI CATG 5 cut(s) 488, 730, 754, 799, 815
FaqI GGGAC 2 cut(s) 205, 694
FatI CATG 5 cut(s) 484, 726, 750, 795, 811
FauI CCCGC 1 cut(s) 104
FbaI TGATCA 1 cut(s) 1008
Fnu4HI GCNGC 5 cut(s) 53, 65, 83, 383, 719
FokI GGATG 4 cut(s) 604, 661, 667, 842
FriOI GRGCYC 1 cut(s) 589
Fsp4HI GCNGC 5 cut(s) 53, 65, 83, 383, 719
FspBI CTAG 1 cut(s) 690
GlaI GCGC 2 cut(s) 166, 805
GluI GCNGC 5 cut(s) 53, 65, 83, 383, 719
GsaI CCCAGC 1 cut(s) 172
GsuI CTGGAG 1 cut(s) 188
HaeII RGCGCY 1 cut(s) 807
HaeIII GGCC 5 cut(s) 95, 152, 209, 475, 969
HapII CCGG 4 cut(s) 441, 602, 650, 704
HgaI GACGC 3 cut(s) 164, 236, 376
HhaI GCGC 2 cut(s) 167, 806
Hin1I GRCGYC 2 cut(s) 156, 228
Hin1II CATG 5 cut(s) 488, 730, 754, 799, 815
Hin6I GCGC 2 cut(s) 165, 804
HinP1I GCGC 2 cut(s) 165, 804
HinfI GANTC 5 cut(s) 14, 143, 820, 859, 940
HpaII CCGG 4 cut(s) 441, 602, 650, 704
HphI GGTGA 3 cut(s) 97, 591, 968
Hpy188I TCNGA 5 cut(s) 148, 241, 362, 412, 538
Hpy188III TCNNGA 6 cut(s) 41, 121, 523, 566, 744, 856
Hpy99I CGWCG 3 cut(s) 158, 197, 230
HpyAV CCTTC 5 cut(s) 170, 286, 513, 810, 995
HpyCH4III ACNGT 1 cut(s) 319
HpyCH4IV ACGT 1 cut(s) 127
HpyCH4V TGCA 5 cut(s) 55, 385, 479, 887, 946
HpyF10VI GCNNNNNNNGC 9 cut(s) 61, 164, 173, 382, 388, 472, 584, 789, 893
HpyF3I CTNAG 2 cut(s) 423, 558
HpySE526I ACGT 1 cut(s) 127
Hsp92I GRCGYC 2 cut(s) 156, 228
Hsp92II CATG 5 cut(s) 488, 730, 754, 799, 815
HspAI GCGC 2 cut(s) 165, 804
Ksp22I TGATCA 1 cut(s) 1008
Kzo9I GATC 7 cut(s) 34, 70, 102, 562, 764, 982, 1008
LguI GCTCTTC 1 cut(s) 293
LmnI GCTCC 5 cut(s) 388, 404, 433, 592, 797
Lsp1109I GCAGC 3 cut(s) 39, 94, 369
LweI GCATC 3 cut(s) 478, 626, 683
MaeI CTAG 1 cut(s) 690
MaeII ACGT 1 cut(s) 127
MaeIII GTNAC 1 cut(s) 566
MalI GATC 7 cut(s) 36, 72, 104, 564, 766, 984, 1010
MboI GATC 7 cut(s) 34, 70, 102, 562, 764, 982, 1008
MboII GAAGA 5 cut(s) 29, 310, 447, 507, 759
MflI RGATCY 2 cut(s) 764, 982
MhlI GDGCHC 2 cut(s) 589, 777
MluCI AATT 2 cut(s) 530, 839
MlyI GAGTC 4 cut(s) 152, 814, 868, 934
MmeI TCCRAC 3 cut(s) 5, 264, 330
MseI TTAA 1 cut(s) 903
MspI CCGG 4 cut(s) 441, 602, 650, 704
MspR9I CCNGG 6 cut(s) 206, 212, 603, 650, 651, 801
Mva1269I GAATGC 1 cut(s) 721
MvaI CCWGG 3 cut(s) 206, 212, 801
MvnI CGCG 2 cut(s) 122, 165
MwoI GCNNNNNNNGC 9 cut(s) 61, 164, 173, 382, 388, 472, 584, 789, 893
NciI CCSGG 3 cut(s) 603, 650, 651
NdeII GATC 7 cut(s) 34, 70, 102, 562, 764, 982, 1008
NlaIII CATG 5 cut(s) 488, 730, 754, 799, 815
NlaIV GGNNCC 3 cut(s) 588, 774, 793
NmeAIII GCCGAG 1 cut(s) 292
NmuCI GTSAC 1 cut(s) 566
NruI TCGCGA 1 cut(s) 122
NspI RCATGY 1 cut(s) 754
NspV TTCGAA 1 cut(s) 506
PaeR7I CTCGAG 3 cut(s) 140, 580, 822
PciSI GCTCTTC 1 cut(s) 293
PcsI WCGNNNNNNNCGW 3 cut(s) 39, 84, 264
PctI GAATGC 1 cut(s) 721
PfeI GAWTC 1 cut(s) 14
PkrI GCNGC 5 cut(s) 54, 66, 84, 384, 720
PleI GAGTC 4 cut(s) 151, 814, 867, 934
PmaCI CACGTG 1 cut(s) 128
PmlI CACGTG 1 cut(s) 128
PpsI GAGTC 4 cut(s) 151, 814, 867, 934
Ppu21I YACGTR 1 cut(s) 128
PshAI GACNNNNGTC 1 cut(s) 128
Psp6I CCWGG 3 cut(s) 204, 210, 799
PspCI CACGTG 1 cut(s) 128
PspFI CCCAGC 1 cut(s) 168
PspGI CCWGG 3 cut(s) 204, 210, 799
PspN4I GGNNCC 3 cut(s) 588, 774, 793
PspPI GGNCC 6 cut(s) 94, 184, 208, 214, 473, 968
PspXI VCTCGAGB 3 cut(s) 140, 580, 822
PstI CTGCAG 2 cut(s) 387, 481
PstNI CAGNNNCTG 1 cut(s) 806
PsuI RGATCY 2 cut(s) 764, 982
RruI TCGCGA 1 cut(s) 122
SapI GCTCTTC 1 cut(s) 293
SaqAI TTAA 1 cut(s) 903
SatI GCNGC 5 cut(s) 53, 65, 83, 383, 719
Sau3AI GATC 7 cut(s) 34, 70, 102, 562, 764, 982, 1008
Sau96I GGNCC 6 cut(s) 94, 184, 208, 214, 473, 968
SchI GAGTC 4 cut(s) 152, 814, 868, 934
ScrFI CCNGG 6 cut(s) 206, 212, 603, 650, 651, 801
SduI GDGCHC 2 cut(s) 589, 777
SfaNI GCATC 3 cut(s) 478, 626, 683
SfcI CTRYAG 2 cut(s) 383, 477
Sfr274I CTCGAG 3 cut(s) 140, 580, 822
SfuI TTCGAA 1 cut(s) 506
SinI GGWCC 2 cut(s) 184, 214
SlaI CTCGAG 3 cut(s) 140, 580, 822
SmaI CCCGGG 1 cut(s) 651
SmlI CTYRAG 5 cut(s) 140, 580, 742, 778, 822
SmoI CTYRAG 5 cut(s) 140, 580, 742, 778, 822
Sse9I AATT 2 cut(s) 530, 839
SsiI CCGC 6 cut(s) 65, 97, 246, 428, 718, 847
SspMI CTAG 1 cut(s) 690
StyD4I CCNGG 6 cut(s) 204, 210, 601, 648, 649, 799
StyI CCWWGG 1 cut(s) 713
TaaI ACNGT 1 cut(s) 319
TaiI ACGT 1 cut(s) 130
TaqI TCGA 9 cut(s) 17, 33, 78, 87, 141, 225, 506, 581, 823
TasI AATT 2 cut(s) 530, 839
TauI GCSGC 2 cut(s) 67, 721
TfiI GAWTC 1 cut(s) 14
Tru1I TTAA 1 cut(s) 903
Tru9I TTAA 1 cut(s) 903
TseFI GTSAC 1 cut(s) 566
TseI GCWGC 3 cut(s) 52, 82, 382
Tsp45I GTSAC 1 cut(s) 566
TspMI CCCGGG 1 cut(s) 649
VpaK11BI GGWCC 2 cut(s) 184, 214
XapI RAATTY 1 cut(s) 839
XceI RCATGY 1 cut(s) 754
XhoI CTCGAG 3 cut(s) 140, 580, 822
XmaI CCCGGG 1 cut(s) 649
XspI CTAG 1 cut(s) 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.