RLG00000007772

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0014233
Physical Location & Seq
Reverse (-)
20873677 .. 20876298
2622 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007772

Sequence Viewer

Length: 1020 bp
ATGCTGGCGAGGGAATCGAGCAGTTCCAACTTCGATCTTCCCGAGGAAGTGCTGCAAGTTCTGCCGCCCGATCCTTTCGAGCAGCTCGATGTGGCCCGCAAGATCACCTCCATTGCCCTCGCGACACGTGTCTCCTCCCTCGAGTCCGAGGCCGACGCTCTTCGCGCCCAGCTCGCCGATAAGGACCACCTCGTCGCCGACCTCCAGGCCCAGGTCCAGTCCCTCGACGCCTCTCTCTCCGACACCGCCGACAAGCTCGCCCTCGCCGAGCAGGAAAAGGAGGAGTTGTTGAAGGAGAAGAGCTACCTGTCCAATACTGTGAGGAAGCTCAACAGAGATGTCTCCAAGTTGGAGGTGTTCAGAAAGACGCTTATGCGTTCCTTGCAGGAGGAGGACGAGAGTGGAGTGGTTGCTAAGGCGGAGGAGCAGACCGAAGAGAGCTTATCTTCTCAATCGCAGACTGGAGCTGCAGATCATGACGCCACTTTACCACCTTCGAGAACATCTTCAATCCAGAGCCAATTCTCTGAAACAGGAAGCACATATGCTGAGGATCGTGACCCAAATGCCTCGAGGGCTCCTCGCATATCACCGGGGCTCCTATTAGCTTCCCAAACCAGCACACCGCGGCTTACTCCCCCTGGTTCTCCTCCTATCTATTCGGCATCCACATCCCCAACTAGGACATCAAAGCCGGTGTCCCCAAGGCGACATTCCATGTCATTTGCTACCTCAAGAGGCATGTTTGATGATAGATCTTCGGTGCCCTCAAGCCATCACGGCTCCATGCCAGGCGCTGAACATGGAAGGACTCGAGTGGATGGGAAAGAATTTTTCCGCCAAGTCAGGAGTCGTTTGTCTTATGAGCAGTTTGGTGCATTTCTGGCAAATGTTAAGGAACTAAATGCCCACAAGCAAACAAAAGAAGAGACTCTGCATAAAGCTGATGAGATATTTGGCCCAGATAATAAGGATCTATATGTCATATTTGAAGGATTGATCAGTCGTAATGTCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0016020 GO:0022857 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

340

Amino Acids

37.35

Weight (kDa)

5.49

Isoelectric Point (pI)

57.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At4g15545_C PF25972 272 - 337 6.1e-34 At4g15545-like, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014233)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15545
fragaria_vesca FvH4_4g16760
malus_domestica MD13G1176600.v1.1 MD16G1178400.v1.1
prunus_persica Prupe.1G145800_v2.0.a1 Prupe.1G145800_v2.0.a1
pyrus_communis pycom13g15240 pycom16g14990
rosa_chinensis RchiOBHm_Chr4g0420331
rosa_laevigata RLG00000007772
rosa_roxburghii Rroxscaffold_5G00362980
rosa_samantha Rh4AG224500 Rh4BG227500 Rh4CG239000 Rh4DG223000
rosa_wichuraiana Rw0G005760 Rw4G019380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 191
AbsI CCTCGAGG 1 cut(s) 571
AccB1I GGYRCC 1 cut(s) 763
AccII CGCG 3 cut(s) 122, 165, 628
AciI CCGC 7 cut(s) 65, 97, 246, 419, 626, 628, 838
AclWI GGATC 3 cut(s) 65, 561, 981
AcsI RAATTY 1 cut(s) 830
AcvI CACGTG 1 cut(s) 128
AcyI GRCGYC 2 cut(s) 228, 480
AfiI CCNNNNNNNGG 2 cut(s) 211, 681
AflIII ACRYGT 2 cut(s) 125, 127
AgsI TTSAA 3 cut(s) 292, 510, 992
AjnI CCWGG 4 cut(s) 204, 210, 640, 790
AjuI GAANNNNNNNTTGG 2 cut(s) 697, 729
AluBI AGCT 9 cut(s) 85, 172, 256, 303, 328, 441, 467, 608, 944
AluI AGCT 9 cut(s) 85, 172, 256, 303, 328, 441, 467, 608, 944
Alw26I GTCTC 3 cut(s) 136, 346, 923
AlwI GGATC 3 cut(s) 65, 561, 981
AlwNI CAGNNNCTG 1 cut(s) 797
Ama87I CYCGRG 4 cut(s) 41, 140, 571, 813
AoxI GGCC 4 cut(s) 93, 150, 207, 958
ApeKI GCWGC 3 cut(s) 52, 82, 467
ApoI RAATTY 1 cut(s) 830
ArsI GACNNNNNNTTYG 2 cut(s) 683, 715
Asp700I GAANNNNTTC 1 cut(s) 505
AspLEI GCGC 2 cut(s) 167, 797
AspS9I GGNCC 5 cut(s) 94, 184, 208, 214, 959
AsuC2I CCSGG 1 cut(s) 594
AsuHPI GGTGA 2 cut(s) 97, 582
AvaI CYCGRG 4 cut(s) 41, 140, 571, 813
AvaII GGWCC 2 cut(s) 184, 214
BaeGI GKGCMC 1 cut(s) 768
BanI GGYRCC 1 cut(s) 763
BanII GRGCYC 2 cut(s) 580, 600
BbrPI CACGTG 1 cut(s) 128
BbvCI CCTCAGC 1 cut(s) 549
BbvI GCAGC 3 cut(s) 39, 94, 454
BccI CCATC 2 cut(s) 783, 815
BceAI ACGGC 1 cut(s) 796
BciT130I CCWGG 4 cut(s) 206, 212, 642, 792
BclI TGATCA 1 cut(s) 999
BcnI CCSGG 1 cut(s) 594
BcoDI GTCTC 3 cut(s) 136, 346, 923
BfaI CTAG 1 cut(s) 681
BfmI CTRYAG 1 cut(s) 468
BfoI RGCGCY 1 cut(s) 798
BglI GCCNNNNNGGC 2 cut(s) 575, 780
BglII AGATCT 1 cut(s) 755
BisI GCNGC 5 cut(s) 53, 65, 83, 468, 629
BlsI GCNGC 5 cut(s) 54, 66, 84, 469, 630
Bme1390I CCNGG 5 cut(s) 206, 212, 594, 642, 792
Bme18I GGWCC 2 cut(s) 184, 214
BmeT110I CYCGRG 4 cut(s) 41, 140, 571, 813
BmgT120I GGNCC 5 cut(s) 94, 184, 208, 214, 959
BmiI GGNNCC 4 cut(s) 579, 599, 765, 784
BmrFI CCNGG 5 cut(s) 206, 212, 594, 642, 792
BmsI GCATC 1 cut(s) 674
BoxI GACNNNNGTC 1 cut(s) 128
BplI GAGNNNNNCTC 2 cut(s) 565, 597
BpmI CTGGAG 2 cut(s) 188, 483
Bpu10I CCTNAGC 2 cut(s) 414, 549
BpuEI CTTGAG 2 cut(s) 718, 754
BpuMI CCSGG 1 cut(s) 594
BsaAI YACGTR 1 cut(s) 128
BsaHI GRCGYC 2 cut(s) 228, 480
BsaJI CCNNGG 7 cut(s) 42, 147, 210, 593, 626, 640, 704
BsaXI ACNNNNNCTCC 4 cut(s) 272, 302, 582, 612
Bsc4I CCNNNNNNNGG 2 cut(s) 211, 681
Bse118I RCCGGY 1 cut(s) 694
Bse1I ACTGG 2 cut(s) 217, 466
Bse3DI GCAATG 1 cut(s) 111
BseBI CCWGG 4 cut(s) 206, 212, 642, 792
BseDI CCNNGG 7 cut(s) 42, 147, 210, 593, 626, 640, 704
BseGI GGATG 3 cut(s) 665, 671, 826
BseLI CCNNNNNNNGG 2 cut(s) 211, 681
BseMI GCAATG 1 cut(s) 111
BseMII CTCAG 1 cut(s) 540
BseNI ACTGG 2 cut(s) 217, 466
BseRI GAGGAG 6 cut(s) 124, 296, 404, 437, 570, 639
BseSI GKGCMC 1 cut(s) 768
BseXI GCAGC 3 cut(s) 39, 94, 454
BseYI CCCAGC 1 cut(s) 168
Bsh1236I CGCG 3 cut(s) 122, 165, 628
BshFI GGCC 4 cut(s) 95, 152, 209, 960
BshNI GGYRCC 1 cut(s) 763
BsiHKCI CYCGRG 4 cut(s) 41, 140, 571, 813
BsiSI CCGG 2 cut(s) 593, 695
BslFI GGGAC 2 cut(s) 205, 685
BslI CCNNNNNNNGG 2 cut(s) 211, 681
BsmAI GTCTC 3 cut(s) 136, 346, 923
BsmFI GGGAC 2 cut(s) 205, 685
BsnI GGCC 4 cut(s) 95, 152, 209, 960
BsoBI CYCGRG 4 cut(s) 41, 140, 571, 813
Bsp1286I GDGCHC 3 cut(s) 580, 600, 768
Bsp143I GATC 8 cut(s) 34, 70, 102, 472, 553, 755, 973, 999
Bsp68I TCGCGA 1 cut(s) 122
BspACI CCGC 7 cut(s) 65, 97, 246, 419, 626, 628, 838
BspANI GGCC 4 cut(s) 95, 152, 209, 960
BspCNI CTCAG 1 cut(s) 541
BspFNI CGCG 3 cut(s) 122, 165, 628
BspHI TCATGA 1 cut(s) 475
BspLI GGNNCC 4 cut(s) 579, 599, 765, 784
BspMAI CTGCAG 1 cut(s) 472
BspPI GGATC 3 cut(s) 65, 561, 981
BspQI GCTCTTC 2 cut(s) 165, 293
BspT107I GGYRCC 1 cut(s) 763
BsrDI GCAATG 1 cut(s) 111
BsrFI RCCGGY 1 cut(s) 694
BsrI ACTGG 2 cut(s) 217, 466
BssAI RCCGGY 1 cut(s) 694
BssECI CCNNGG 7 cut(s) 42, 147, 210, 593, 626, 640, 704
BssMI GATC 8 cut(s) 34, 70, 102, 472, 553, 755, 973, 999
BssNI GRCGYC 2 cut(s) 228, 480
BssT1I CCWWGG 1 cut(s) 704
Bst2UI CCWGG 4 cut(s) 206, 212, 642, 792
Bst4CI ACNGT 1 cut(s) 319
Bst6I CTCTTC 4 cut(s) 165, 293, 429, 921
BstACI GRCGYC 2 cut(s) 228, 480
BstAPI GCANNNNNTGC 1 cut(s) 61
BstBAI YACGTR 1 cut(s) 128
BstC8I GCNNGC 4 cut(s) 6, 97, 174, 258
BstDEI CTNAG 2 cut(s) 414, 549
BstDSI CCRYGG 1 cut(s) 626
BstF5I GGATG 3 cut(s) 665, 671, 826
BstFNI CGCG 3 cut(s) 122, 165, 628
BstH2I RGCGCY 1 cut(s) 798
BstHHI GCGC 2 cut(s) 167, 797
BstKTI GATC 8 cut(s) 37, 73, 105, 475, 556, 758, 976, 1002
BstMAI GTCTC 3 cut(s) 136, 346, 923
BstMBI GATC 8 cut(s) 34, 70, 102, 472, 553, 755, 973, 999
BstMWI GCNNNNNNNGC 7 cut(s) 61, 164, 173, 382, 575, 780, 884
BstNI CCWGG 4 cut(s) 206, 212, 642, 792
BstNSI RCATGY 1 cut(s) 745
BstPAI GACNNNNGTC 1 cut(s) 128
BstSCI CCNGG 5 cut(s) 204, 210, 592, 640, 790
BstSFI CTRYAG 1 cut(s) 468
BstSLI GKGCMC 1 cut(s) 768
BstUI CGCG 3 cut(s) 122, 165, 628
BstV1I GCAGC 3 cut(s) 39, 94, 454
BstX2I RGATCY 2 cut(s) 755, 973
BstYI RGATCY 2 cut(s) 755, 973
BsuRI GGCC 4 cut(s) 95, 152, 209, 960
BtgI CCRYGG 1 cut(s) 626
BtsCI GGATG 3 cut(s) 665, 671, 826
BtuMI TCGCGA 1 cut(s) 122
Cac8I GCNNGC 4 cut(s) 6, 97, 174, 258
CaiI CAGNNNCTG 1 cut(s) 797
CciI TCATGA 1 cut(s) 475
CfoI GCGC 2 cut(s) 167, 797
Cfr10I RCCGGY 1 cut(s) 694
Cfr13I GGNCC 5 cut(s) 94, 184, 208, 214, 959
Cfr42I CCGCGG 1 cut(s) 629
CseI GACGC 4 cut(s) 164, 236, 376, 488
CviAII CATG 5 cut(s) 476, 718, 742, 787, 803
DdeI CTNAG 2 cut(s) 414, 549
DpnI GATC 8 cut(s) 36, 72, 104, 474, 555, 757, 975, 1001
DpnII GATC 8 cut(s) 34, 70, 102, 472, 553, 755, 973, 999
DrdI GACNNNNNNGTC 1 cut(s) 191
DseDI GACNNNNNNGTC 1 cut(s) 191
Eam1104I CTCTTC 4 cut(s) 165, 293, 429, 921
EarI CTCTTC 4 cut(s) 165, 293, 429, 921
EciI GGCGGA 2 cut(s) 434, 827
Eco130I CCWWGG 1 cut(s) 704
Eco24I GRGCYC 2 cut(s) 580, 600
Eco47I GGWCC 2 cut(s) 184, 214
Eco72I CACGTG 1 cut(s) 128
Eco88I CYCGRG 4 cut(s) 41, 140, 571, 813
EcoRII CCWGG 4 cut(s) 204, 210, 640, 790
EcoT14I CCWWGG 1 cut(s) 704
EcoT38I GRGCYC 2 cut(s) 580, 600
ErhI CCWWGG 1 cut(s) 704
FaeI CATG 5 cut(s) 479, 721, 745, 790, 806
FaqI GGGAC 2 cut(s) 205, 685
FatI CATG 5 cut(s) 475, 717, 741, 786, 802
FauI CCCGC 1 cut(s) 104
FauNDI CATATG 1 cut(s) 544
FbaI TGATCA 1 cut(s) 999
Fnu4HI GCNGC 5 cut(s) 53, 65, 83, 468, 629
FokI GGATG 3 cut(s) 652, 658, 833
FriOI GRGCYC 2 cut(s) 580, 600
Fsp4HI GCNGC 5 cut(s) 53, 65, 83, 468, 629
FspBI CTAG 1 cut(s) 681
GlaI GCGC 2 cut(s) 166, 796
GluI GCNGC 5 cut(s) 53, 65, 83, 468, 629
GsaI CCCAGC 1 cut(s) 172
GsuI CTGGAG 2 cut(s) 188, 483
HaeII RGCGCY 1 cut(s) 798
HaeIII GGCC 4 cut(s) 95, 152, 209, 960
HapII CCGG 2 cut(s) 593, 695
HgaI GACGC 4 cut(s) 164, 236, 376, 488
HhaI GCGC 2 cut(s) 167, 797
Hin1I GRCGYC 2 cut(s) 228, 480
Hin1II CATG 5 cut(s) 479, 721, 745, 790, 806
Hin6I GCGC 2 cut(s) 165, 795
HinP1I GCGC 2 cut(s) 165, 795
HinfI GANTC 5 cut(s) 14, 143, 811, 850, 931
HpaII CCGG 2 cut(s) 593, 695
HphI GGTGA 2 cut(s) 97, 582
Hpy188I TCNGA 4 cut(s) 148, 241, 362, 529
Hpy188III TCNNGA 8 cut(s) 41, 121, 476, 498, 514, 557, 735, 847
Hpy99I CGWCG 3 cut(s) 158, 197, 230
HpyAV CCTTC 4 cut(s) 286, 504, 801, 986
HpyCH4III ACNGT 1 cut(s) 319
HpyCH4IV ACGT 1 cut(s) 127
HpyCH4V TGCA 5 cut(s) 55, 385, 470, 878, 937
HpyF10VI GCNNNNNNNGC 7 cut(s) 61, 164, 173, 382, 575, 780, 884
HpyF3I CTNAG 2 cut(s) 414, 549
HpySE526I ACGT 1 cut(s) 127
Hsp92I GRCGYC 2 cut(s) 228, 480
Hsp92II CATG 5 cut(s) 479, 721, 745, 790, 806
HspAI GCGC 2 cut(s) 165, 795
Ksp22I TGATCA 1 cut(s) 999
KspI CCGCGG 1 cut(s) 629
Kzo9I GATC 8 cut(s) 34, 70, 102, 472, 553, 755, 973, 999
LguI GCTCTTC 2 cut(s) 165, 293
LmnI GCTCC 5 cut(s) 424, 464, 583, 603, 788
Lsp1109I GCAGC 3 cut(s) 39, 94, 454
LweI GCATC 1 cut(s) 674
MaeI CTAG 1 cut(s) 681
MaeII ACGT 1 cut(s) 127
MaeIII GTNAC 1 cut(s) 557
MalI GATC 8 cut(s) 36, 72, 104, 474, 555, 757, 975, 1001
MboI GATC 8 cut(s) 34, 70, 102, 472, 553, 755, 973, 999
MboII GAAGA 8 cut(s) 29, 152, 310, 438, 446, 498, 750, 938
MflI RGATCY 2 cut(s) 755, 973
MhlI GDGCHC 3 cut(s) 580, 600, 768
MluCI AATT 2 cut(s) 521, 830
MlyI GAGTC 4 cut(s) 152, 805, 859, 925
MmeI TCCRAC 3 cut(s) 51, 264, 330
MroXI GAANNNNTTC 1 cut(s) 505
MseI TTAA 1 cut(s) 894
MspA1I CMGCKG 1 cut(s) 628
MspI CCGG 2 cut(s) 593, 695
MspR9I CCNGG 5 cut(s) 206, 212, 594, 642, 792
MvaI CCWGG 4 cut(s) 206, 212, 642, 792
MvnI CGCG 3 cut(s) 122, 165, 628
MwoI GCNNNNNNNGC 7 cut(s) 61, 164, 173, 382, 575, 780, 884
NciI CCSGG 1 cut(s) 594
NdeI CATATG 1 cut(s) 544
NdeII GATC 8 cut(s) 34, 70, 102, 472, 553, 755, 973, 999
NlaIII CATG 5 cut(s) 479, 721, 745, 790, 806
NlaIV GGNNCC 4 cut(s) 579, 599, 765, 784
NmeAIII GCCGAG 1 cut(s) 292
NmuCI GTSAC 1 cut(s) 557
NruI TCGCGA 1 cut(s) 122
NspI RCATGY 1 cut(s) 745
PaeR7I CTCGAG 3 cut(s) 140, 571, 813
PagI TCATGA 1 cut(s) 475
PciSI GCTCTTC 2 cut(s) 165, 293
PcsI WCGNNNNNNNCGW 3 cut(s) 39, 84, 264
PdmI GAANNNNTTC 1 cut(s) 505
PfeI GAWTC 1 cut(s) 14
PkrI GCNGC 5 cut(s) 54, 66, 84, 469, 630
PleI GAGTC 4 cut(s) 151, 805, 858, 925
PmaCI CACGTG 1 cut(s) 128
PmlI CACGTG 1 cut(s) 128
PpsI GAGTC 4 cut(s) 151, 805, 858, 925
Ppu21I YACGTR 1 cut(s) 128
PshAI GACNNNNGTC 1 cut(s) 128
Psp6I CCWGG 4 cut(s) 204, 210, 640, 790
PspCI CACGTG 1 cut(s) 128
PspFI CCCAGC 1 cut(s) 168
PspGI CCWGG 4 cut(s) 204, 210, 640, 790
PspN4I GGNNCC 4 cut(s) 579, 599, 765, 784
PspPI GGNCC 5 cut(s) 94, 184, 208, 214, 959
PspXI VCTCGAGB 3 cut(s) 140, 571, 813
PstI CTGCAG 1 cut(s) 472
PstNI CAGNNNCTG 1 cut(s) 797
PsuI RGATCY 2 cut(s) 755, 973
RruI TCGCGA 1 cut(s) 122
SacII CCGCGG 1 cut(s) 629
SapI GCTCTTC 2 cut(s) 165, 293
SaqAI TTAA 1 cut(s) 894
SatI GCNGC 5 cut(s) 53, 65, 83, 468, 629
Sau3AI GATC 8 cut(s) 34, 70, 102, 472, 553, 755, 973, 999
Sau96I GGNCC 5 cut(s) 94, 184, 208, 214, 959
SchI GAGTC 4 cut(s) 152, 805, 859, 925
ScrFI CCNGG 5 cut(s) 206, 212, 594, 642, 792
SduI GDGCHC 3 cut(s) 580, 600, 768
SfaNI GCATC 1 cut(s) 674
SfcI CTRYAG 1 cut(s) 468
Sfr274I CTCGAG 3 cut(s) 140, 571, 813
Sfr303I CCGCGG 1 cut(s) 629
SgrBI CCGCGG 1 cut(s) 629
SinI GGWCC 2 cut(s) 184, 214
SlaI CTCGAG 3 cut(s) 140, 571, 813
SmlI CTYRAG 5 cut(s) 140, 571, 733, 769, 813
SmoI CTYRAG 5 cut(s) 140, 571, 733, 769, 813
Sse9I AATT 2 cut(s) 521, 830
SsiI CCGC 7 cut(s) 65, 97, 246, 419, 626, 628, 838
SspMI CTAG 1 cut(s) 681
StyD4I CCNGG 5 cut(s) 204, 210, 592, 640, 790
StyI CCWWGG 1 cut(s) 704
TaaI ACNGT 1 cut(s) 319
TaiI ACGT 1 cut(s) 130
TaqI TCGA 9 cut(s) 17, 33, 78, 87, 141, 225, 497, 572, 814
TaqII GACCGA 1 cut(s) 446
TasI AATT 2 cut(s) 521, 830
TauI GCSGC 2 cut(s) 67, 631
TfiI GAWTC 1 cut(s) 14
Tru1I TTAA 1 cut(s) 894
Tru9I TTAA 1 cut(s) 894
TseFI GTSAC 1 cut(s) 557
TseI GCWGC 3 cut(s) 52, 82, 467
Tsp45I GTSAC 1 cut(s) 557
VpaK11BI GGWCC 2 cut(s) 184, 214
XapI RAATTY 1 cut(s) 830
XceI RCATGY 1 cut(s) 745
XhoI CTCGAG 3 cut(s) 140, 571, 813
XmnI GAANNNNTTC 1 cut(s) 505
XspI CTAG 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.