FvH4_4g18950

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0015599
Physical Location & Seq
Reverse (-)
22710771 .. 22712305
1535 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g18950.t1

Sequence Viewer

Length: 918 bp
ATGGTTCCAGAATTAGAGAAACCTCGGATTACCGAGATACATGTCCGGATGGACTGCAATGGATGTGTACAGAAGATTAAGAAAGCACTACATGGCATCAGTGGTATATATGATCTCTACATTGACTTCCCTCAGCAGAAGTTGACAATAATAGGGTGGGCGGATCCAGAGAAGATAGTAAAAGCCATAAAGAAAACAAGGAAGATTGCCACCATATGTTCCCATATAGAGCAACCAACAGAGCCTGCTCCTCCTCCAGCAGAACAACCCCCTGAAGGGGGCGCAGCACCACCTCCCGATGCAGCAAACCCTCCAACAGAATCACCTCCACCAGCCGAACCAACTCCACCTCCTGAAGCTGTTCCACCAGCCGAGCCACCAAAGGACCCGCCACCACCGGAACATGCACCACCACCCCGTCCACCCGAGGCACTACCAACACCTGTGGCTGCTGAGACCAATCTAGGCCAACAACATCCGGTGTACCACCACAGACCAAGAGAAGTTGGGGAAGTTCGTACCATACACCACTACCCTCCTGATTATGGCTACAGATACGGCTACCCTAATGGCTACCCCGGGTACTATAACAGATACCATAATAATCAAGGACCTCCACCGGAACCAACTCCTCCCCCTGCCCAGGCTCCTTCTCCTTCTCCTCCGGTTCAGGTCCATGCACCACCGCCTGTCTATGTGACACATAGCTATAACACATACAGGCCATCACCTTATGTCACTGAATACGAGTATGTTCAACCACCCCCACCACAACAGACGCATTACAGTAGGATCAGCCAATACCACCACTACAATGAGGATCAACCATATCACAATGTCAGCAGCAATGGAAATGGAAATGGCAATATCACATCTATTTTTAGCGATGAAAATCCGAACGCGTGTGCTGTAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

306

Amino Acids

33.76

Weight (kDa)

5.95

Isoelectric Point (pI)

69.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 15 - 66 2.7e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015599)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g18950
malus_domestica MD13G1161800.v1.1 MD16G1161600.v1.1
prunus_persica Prupe.1G129100_v2.0.a1
pyrus_communis pycom16g13700
rosa_chinensis RchiOBHm_Chr4g0423841
rosa_laevigata RLG00000007527
rosa_multiflora Rmu_sc0003369.1_g000009 Rmu_sc0008198.1_g000001
rosa_roxburghii Rroxscaffold_5G00365960
rosa_rugosa Rorug04G0191200 Rorug04G0191200
rosa_samantha Rh4AG250600 Rh4BG255900 Rh4DG250400
rosa_wichuraiana Rw4G021470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 902
AccIII TCCGGA 1 cut(s) 45
AciI CCGC 3 cut(s) 161, 389, 686
AclWI GGATC 4 cut(s) 158, 171, 800, 828
AcuI CTGAAG 2 cut(s) 294, 375
AfaI GTAC 4 cut(s) 69, 485, 520, 584
AfiI CCNNNNNNNGG 6 cut(s) 275, 276, 277, 278, 545, 643
AflIII ACRYGT 2 cut(s) 40, 900
AgsI TTSAA 1 cut(s) 758
AjnI CCWGG 1 cut(s) 642
AluBI AGCT 2 cut(s) 359, 708
AluI AGCT 2 cut(s) 359, 708
Alw26I GTCTC 1 cut(s) 449
AlwI GGATC 4 cut(s) 158, 171, 800, 828
AlwNI CAGNNNCTG 1 cut(s) 245
Ama87I CYCGRG 2 cut(s) 425, 578
Aor13HI TCCGGA 1 cut(s) 45
AoxI GGCC 2 cut(s) 466, 722
ApeKI GCWGC 4 cut(s) 284, 302, 449, 843
Asp700I GAANNNNTTC 1 cut(s) 360
AspLEI GCGC 1 cut(s) 284
AspS9I GGNCC 3 cut(s) 385, 611, 673
AsuC2I CCSGG 2 cut(s) 579, 580
AsuHPI GGTGA 2 cut(s) 315, 720
AvaI CYCGRG 2 cut(s) 425, 578
AvaII GGWCC 3 cut(s) 385, 611, 673
BamHI GGATCC 1 cut(s) 163
BbvCI CCTCAGC 1 cut(s) 132
BbvI GCAGC 4 cut(s) 296, 314, 436, 855
BccI CCATC 2 cut(s) 43, 733
BceAI ACGGC 1 cut(s) 574
BciT130I CCWGG 1 cut(s) 644
BcnI CCSGG 2 cut(s) 579, 580
BcoDI GTCTC 1 cut(s) 449
BfaI CTAG 1 cut(s) 464
BfmI CTRYAG 1 cut(s) 550
BisI GCNGC 4 cut(s) 285, 303, 450, 844
BlsI GCNGC 4 cut(s) 286, 304, 451, 845
Bme1390I CCNGG 3 cut(s) 579, 580, 644
Bme18I GGWCC 3 cut(s) 385, 611, 673
BmeT110I CYCGRG 2 cut(s) 425, 578
BmgT120I GGNCC 3 cut(s) 385, 611, 673
BmiI GGNNCC 5 cut(s) 6, 165, 387, 624, 648
BmrFI CCNGG 3 cut(s) 579, 580, 644
BmsI GCATC 2 cut(s) 105, 289
BpmI CTGGAG 1 cut(s) 240
Bpu10I CCTNAGC 1 cut(s) 132
BpuMI CCSGG 2 cut(s) 579, 580
BsaBI GATNNNNATC 1 cut(s) 891
BsaI GGTCTC 1 cut(s) 449
BsaJI CCNNGG 5 cut(s) 23, 426, 577, 578, 642
BsaWI WCCGGW 5 cut(s) 45, 397, 478, 619, 664
BsaXI ACNNNNNCTCC 2 cut(s) 334, 364
Bsc4I CCNNNNNNNGG 6 cut(s) 275, 276, 277, 278, 545, 643
Bse3DI GCAATG 2 cut(s) 64, 853
Bse8I GATNNNNATC 1 cut(s) 891
BseAI TCCGGA 1 cut(s) 45
BseBI CCWGG 1 cut(s) 644
BseDI CCNNGG 5 cut(s) 23, 426, 577, 578, 642
BseGI GGATG 3 cut(s) 54, 68, 475
BseJI GATNNNNATC 1 cut(s) 891
BseLI CCNNNNNNNGG 6 cut(s) 275, 276, 277, 278, 545, 643
BseMI GCAATG 2 cut(s) 64, 853
BseMII CTCAG 2 cut(s) 146, 444
BseRI GAGGAG 4 cut(s) 240, 243, 621, 651
BseXI GCAGC 4 cut(s) 296, 314, 436, 855
Bsh1236I CGCG 1 cut(s) 902
BshFI GGCC 2 cut(s) 468, 724
BsiHKCI CYCGRG 2 cut(s) 425, 578
BsiSI CCGG 6 cut(s) 46, 398, 479, 579, 620, 665
BslI CCNNNNNNNGG 6 cut(s) 275, 276, 277, 278, 545, 643
BsmAI GTCTC 1 cut(s) 449
BsnI GGCC 2 cut(s) 468, 724
Bso31I GGTCTC 1 cut(s) 449
BsoBI CYCGRG 2 cut(s) 425, 578
Bsp13I TCCGGA 1 cut(s) 45
Bsp1407I TGTACA 1 cut(s) 67
Bsp143I GATC 4 cut(s) 112, 163, 792, 820
BspACI CCGC 3 cut(s) 161, 389, 686
BspANI GGCC 2 cut(s) 468, 724
BspCNI CTCAG 2 cut(s) 145, 445
BspEI TCCGGA 1 cut(s) 45
BspFNI CGCG 1 cut(s) 902
BspLI GGNNCC 5 cut(s) 6, 165, 387, 624, 648
BspPI GGATC 4 cut(s) 158, 171, 800, 828
BspTNI GGTCTC 1 cut(s) 449
BsrDI GCAATG 2 cut(s) 64, 853
BsrGI TGTACA 1 cut(s) 67
BssECI CCNNGG 5 cut(s) 23, 426, 577, 578, 642
BssMI GATC 4 cut(s) 112, 163, 792, 820
Bst2UI CCWGG 1 cut(s) 644
Bst4CI ACNGT 1 cut(s) 788
BstAUI TGTACA 1 cut(s) 67
BstC8I GCNNGC 1 cut(s) 246
BstDEI CTNAG 2 cut(s) 132, 453
BstF5I GGATG 3 cut(s) 54, 68, 475
BstFNI CGCG 1 cut(s) 902
BstHHI GCGC 1 cut(s) 284
BstKTI GATC 4 cut(s) 115, 166, 795, 823
BstMAI GTCTC 1 cut(s) 449
BstMBI GATC 4 cut(s) 112, 163, 792, 820
BstNI CCWGG 1 cut(s) 644
BstNSI RCATGY 2 cut(s) 44, 407
BstSCI CCNGG 3 cut(s) 577, 578, 642
BstSFI CTRYAG 1 cut(s) 550
BstUI CGCG 1 cut(s) 902
BstV1I GCAGC 4 cut(s) 296, 314, 436, 855
BstX2I RGATCY 1 cut(s) 163
BstYI RGATCY 1 cut(s) 163
BsuRI GGCC 2 cut(s) 468, 724
BtgZI GCGATG 1 cut(s) 900
BtsCI GGATG 3 cut(s) 54, 68, 475
BtsIMutI CAGTG 2 cut(s) 106, 738
Cac8I GCNNGC 1 cut(s) 246
CaiI CAGNNNCTG 1 cut(s) 245
CfoI GCGC 1 cut(s) 284
Cfr13I GGNCC 3 cut(s) 385, 611, 673
Cfr9I CCCGGG 1 cut(s) 578
CseI GACGC 1 cut(s) 787
Csp6I GTAC 4 cut(s) 68, 484, 519, 583
CspCI CAANNNNNGTGG 2 cut(s) 426, 461
CviAII CATG 4 cut(s) 41, 92, 404, 677
CviQI GTAC 4 cut(s) 68, 484, 519, 583
DdeI CTNAG 2 cut(s) 132, 453
DpnI GATC 4 cut(s) 114, 165, 794, 822
DpnII GATC 4 cut(s) 112, 163, 792, 820
EciI GGCGGA 1 cut(s) 176
Eco31I GGTCTC 1 cut(s) 449
Eco47I GGWCC 3 cut(s) 385, 611, 673
Eco57I CTGAAG 2 cut(s) 294, 375
Eco88I CYCGRG 2 cut(s) 425, 578
EcoO109I RGGNCCY 2 cut(s) 385, 611
EcoRII CCWGG 1 cut(s) 642
FaeI CATG 4 cut(s) 44, 95, 407, 680
FatI CATG 4 cut(s) 40, 91, 403, 676
FauI CCCGC 1 cut(s) 396
FauNDI CATATG 1 cut(s) 215
Fnu4HI GCNGC 4 cut(s) 285, 303, 450, 844
FokI GGATG 3 cut(s) 61, 75, 462
Fsp4HI GCNGC 4 cut(s) 285, 303, 450, 844
FspBI CTAG 1 cut(s) 464
GlaI GCGC 1 cut(s) 283
GluI GCNGC 4 cut(s) 285, 303, 450, 844
GsuI CTGGAG 1 cut(s) 240
HaeIII GGCC 2 cut(s) 468, 724
HapII CCGG 6 cut(s) 46, 398, 479, 579, 620, 665
HgaI GACGC 1 cut(s) 787
HhaI GCGC 1 cut(s) 284
Hin1II CATG 4 cut(s) 44, 95, 407, 680
Hin6I GCGC 1 cut(s) 282
HinP1I GCGC 1 cut(s) 282
HincII GTYRAC 1 cut(s) 144
HindII GTYRAC 1 cut(s) 144
HinfI GANTC 1 cut(s) 320
HpaII CCGG 6 cut(s) 46, 398, 479, 579, 620, 665
HphI GGTGA 2 cut(s) 315, 720
Hpy166II GTNNAC 4 cut(s) 68, 144, 422, 484
Hpy188I TCNGA 2 cut(s) 27, 897
Hpy188III TCNNGA 6 cut(s) 8, 46, 167, 296, 353, 539
Hpy8I GTNNAC 4 cut(s) 68, 144, 422, 484
HpyAV CCTTC 3 cut(s) 269, 660, 666
HpyCH4III ACNGT 1 cut(s) 788
HpyCH4V TGCA 4 cut(s) 57, 302, 407, 680
HpyF3I CTNAG 2 cut(s) 132, 453
Hsp92II CATG 4 cut(s) 44, 95, 407, 680
HspAI GCGC 1 cut(s) 282
Kpn2I TCCGGA 1 cut(s) 45
Kzo9I GATC 4 cut(s) 112, 163, 792, 820
LmnI GCTCC 2 cut(s) 253, 652
Lsp1109I GCAGC 4 cut(s) 296, 314, 436, 855
LweI GCATC 2 cut(s) 105, 289
MaeI CTAG 1 cut(s) 464
MaeIII GTNAC 2 cut(s) 697, 736
MalI GATC 4 cut(s) 114, 165, 794, 822
MboI GATC 4 cut(s) 112, 163, 792, 820
MboII GAAGA 3 cut(s) 85, 184, 214
MflI RGATCY 1 cut(s) 163
MluCI AATT 1 cut(s) 11
MluI ACGCGT 1 cut(s) 900
MmeI TCCRAC 1 cut(s) 338
MroI TCCGGA 1 cut(s) 45
MroXI GAANNNNTTC 1 cut(s) 360
MseI TTAA 1 cut(s) 78
MslI CAYNNNNRTG 1 cut(s) 813
MspI CCGG 6 cut(s) 46, 398, 479, 579, 620, 665
MspR9I CCNGG 3 cut(s) 579, 580, 644
MvaI CCWGG 1 cut(s) 644
MvnI CGCG 1 cut(s) 902
NciI CCSGG 2 cut(s) 579, 580
NdeI CATATG 1 cut(s) 215
NdeII GATC 4 cut(s) 112, 163, 792, 820
NlaIII CATG 4 cut(s) 44, 95, 407, 680
NlaIV GGNNCC 5 cut(s) 6, 165, 387, 624, 648
NmeAIII GCCGAG 1 cut(s) 397
NmuCI GTSAC 2 cut(s) 697, 736
NspI RCATGY 2 cut(s) 44, 407
PciI ACATGT 1 cut(s) 40
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 1 cut(s) 320
PkrI GCNGC 4 cut(s) 286, 304, 451, 845
PpuMI RGGWCCY 2 cut(s) 385, 611
PscI ACATGT 1 cut(s) 40
Psp5II RGGWCCY 2 cut(s) 385, 611
Psp6I CCWGG 1 cut(s) 642
PspGI CCWGG 1 cut(s) 642
PspN4I GGNNCC 5 cut(s) 6, 165, 387, 624, 648
PspPI GGNCC 3 cut(s) 385, 611, 673
PspPPI RGGWCCY 2 cut(s) 385, 611
PstNI CAGNNNCTG 1 cut(s) 245
PsuI RGATCY 1 cut(s) 163
RsaI GTAC 4 cut(s) 69, 485, 520, 584
RsaNI GTAC 4 cut(s) 68, 484, 519, 583
RseI CAYNNNNRTG 1 cut(s) 813
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 4 cut(s) 285, 303, 450, 844
Sau3AI GATC 4 cut(s) 112, 163, 792, 820
Sau96I GGNCC 3 cut(s) 385, 611, 673
ScrFI CCNGG 3 cut(s) 579, 580, 644
SfaNI GCATC 2 cut(s) 105, 289
SfcI CTRYAG 1 cut(s) 550
SinI GGWCC 3 cut(s) 385, 611, 673
SmaI CCCGGG 1 cut(s) 580
SmiMI CAYNNNNRTG 1 cut(s) 813
Sse9I AATT 1 cut(s) 11
SsiI CCGC 3 cut(s) 161, 389, 686
SspMI CTAG 1 cut(s) 464
StyD4I CCNGG 3 cut(s) 577, 578, 642
TaaI ACNGT 1 cut(s) 788
TasI AATT 1 cut(s) 11
TatI WGTACW 1 cut(s) 67
TfiI GAWTC 1 cut(s) 320
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TscAI CASTG 2 cut(s) 106, 745
TseFI GTSAC 2 cut(s) 697, 736
TseI GCWGC 4 cut(s) 284, 302, 449, 843
Tsp45I GTSAC 2 cut(s) 697, 736
TspDTI ATGAA 1 cut(s) 903
TspMI CCCGGG 1 cut(s) 578
TspRI CASTG 2 cut(s) 106, 745
VpaK11BI GGWCC 3 cut(s) 385, 611, 673
XceI RCATGY 2 cut(s) 44, 407
XmaI CCCGGG 1 cut(s) 578
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.