Rroxscaffold_5G00365960

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0015599
Physical Location & Seq
Reverse (-)
47682076 .. 47683496
1421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00365960.1

Sequence Viewer

Length: 684 bp
ATGTTCCCATATAGAACAACCAACAGATCCTGCTTCTCCTCCAGCAGAACAACCCCCGAAGGTGCCGCAGCACCACCTCCCGATGCAGCAAACCCTCCTCCAACAGAATCACCCCCACCAGCCGAACCAGCTCCACCTCCTGAAGCTGCCCCACCAGCCGAGCCACCAAAGGATCCGCCACCACCGGAACATCCACAACCAGAGGCACTACCAACACCTGTGGCTGCTGAGACCAATCTAGGCCAACAACATCCGGTGTACCATCACAGGCCAAGAGAAGTTGGGGAAGTTCATACCATATACCACCACCCACCTGATTATGGCTACAGATACGGCTACCCTAATGGCTACCCCGGCTACTATAACAGATACCATAATAGTCAAGGACCTCCACCGGAACCAACTCCTCCCCCTGCCCCGGCTCCGGCTCCCGCTCAAGTTCCAGTCCATACACCACCGGTCTATGTGACACATAGCTACAACACATACAGGCCATCACCTTATGTCACTGAATATGAGTATGTTCAGCCACCCCCACCACAACAGACGCATTACAGTAGGGTCAGCCAATACCACCACTACAATCAGGATCAGCCGTATCACAATATCAGCAATGGAAATGGAAACGGCAACATCACGTCGATTTTTAGCGACGAAAATCCGAATGCGTGTGCTGTAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

227

Amino Acids

25.05

Weight (kDa)

5.81

Isoelectric Point (pI)

75.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015599)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g18950
malus_domestica MD13G1161800.v1.1 MD16G1161600.v1.1
prunus_persica Prupe.1G129100_v2.0.a1
pyrus_communis pycom16g13700
rosa_chinensis RchiOBHm_Chr4g0423841
rosa_laevigata RLG00000007527
rosa_multiflora Rmu_sc0003369.1_g000009 Rmu_sc0008198.1_g000001
rosa_roxburghii Rroxscaffold_5G00365960
rosa_rugosa Rorug04G0191200 Rorug04G0191200
rosa_samantha Rh4AG250600 Rh4BG255900 Rh4DG250400
rosa_wichuraiana Rw4G021470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 62
AccBSI CCGCTC 1 cut(s) 434
AciI CCGC 3 cut(s) 66, 176, 432
AclWI GGATC 4 cut(s) 21, 167, 180, 597
AcuI CTGAAG 1 cut(s) 162
AfaI GTAC 1 cut(s) 260
AfiI CCNNNNNNNGG 3 cut(s) 320, 418, 424
AgeI ACCGGT 1 cut(s) 457
AjiI CACGTC 1 cut(s) 639
AluBI AGCT 3 cut(s) 131, 146, 477
AluI AGCT 3 cut(s) 131, 146, 477
Alw26I GTCTC 1 cut(s) 224
AlwI GGATC 4 cut(s) 21, 167, 180, 597
AlwNI CAGNNNCTG 1 cut(s) 30
AoxI GGCC 3 cut(s) 241, 269, 491
ApeKI GCWGC 4 cut(s) 68, 86, 146, 224
AsiGI ACCGGT 1 cut(s) 457
AspS9I GGNCC 1 cut(s) 386
AsuC2I CCSGG 2 cut(s) 354, 419
AsuHPI GGTGA 2 cut(s) 102, 489
AvaII GGWCC 1 cut(s) 386
BamHI GGATCC 1 cut(s) 172
BanI GGYRCC 1 cut(s) 62
BbvI GCAGC 4 cut(s) 80, 98, 133, 211
BccI CCATC 2 cut(s) 270, 502
BceAI ACGGC 3 cut(s) 349, 580, 643
BcnI CCSGG 2 cut(s) 354, 419
BcoDI GTCTC 1 cut(s) 224
BfaI CTAG 1 cut(s) 239
BfmI CTRYAG 1 cut(s) 325
BisI GCNGC 5 cut(s) 66, 69, 87, 147, 225
BlsI GCNGC 5 cut(s) 67, 70, 88, 148, 226
Bme1390I CCNGG 2 cut(s) 354, 419
Bme18I GGWCC 1 cut(s) 386
BmgBI CACGTC 1 cut(s) 639
BmgT120I GGNCC 1 cut(s) 386
BmiI GGNNCC 5 cut(s) 64, 174, 399, 423, 429
BmrFI CCNGG 2 cut(s) 354, 419
BmsI GCATC 1 cut(s) 73
BpmI CTGGAG 1 cut(s) 25
BpuEI CTTGAG 1 cut(s) 420
BpuMI CCSGG 2 cut(s) 354, 419
BsaI GGTCTC 1 cut(s) 224
BsaJI CCNNGG 2 cut(s) 352, 417
BsaWI WCCGGW 4 cut(s) 184, 253, 394, 457
Bsc4I CCNNNNNNNGG 3 cut(s) 320, 418, 424
Bse118I RCCGGY 1 cut(s) 457
Bse1I ACTGG 1 cut(s) 443
Bse3DI GCAATG 1 cut(s) 619
BseDI CCNNGG 2 cut(s) 352, 417
BseGI GGATG 2 cut(s) 190, 250
BseLI CCNNNNNNNGG 3 cut(s) 320, 418, 424
BseMI GCAATG 1 cut(s) 619
BseMII CTCAG 1 cut(s) 219
BseNI ACTGG 1 cut(s) 443
BseRI GAGGAG 3 cut(s) 28, 87, 396
BseXI GCAGC 4 cut(s) 80, 98, 133, 211
BshFI GGCC 3 cut(s) 243, 271, 493
BshNI GGYRCC 1 cut(s) 62
BshTI ACCGGT 1 cut(s) 457
BsiSI CCGG 7 cut(s) 185, 254, 354, 395, 419, 425, 458
BslI CCNNNNNNNGG 3 cut(s) 320, 418, 424
BsmAI GTCTC 1 cut(s) 224
BsmI GAATGC 1 cut(s) 670
BsnI GGCC 3 cut(s) 243, 271, 493
Bso31I GGTCTC 1 cut(s) 224
Bsp143I GATC 3 cut(s) 26, 172, 589
BspACI CCGC 3 cut(s) 66, 176, 432
BspANI GGCC 3 cut(s) 243, 271, 493
BspCNI CTCAG 1 cut(s) 220
BspLI GGNNCC 5 cut(s) 64, 174, 399, 423, 429
BspPI GGATC 4 cut(s) 21, 167, 180, 597
BspT107I GGYRCC 1 cut(s) 62
BspTNI GGTCTC 1 cut(s) 224
BsrBI CCGCTC 1 cut(s) 434
BsrDI GCAATG 1 cut(s) 619
BsrFI RCCGGY 1 cut(s) 457
BsrI ACTGG 1 cut(s) 443
BssAI RCCGGY 1 cut(s) 457
BssECI CCNNGG 2 cut(s) 352, 417
BssMI GATC 3 cut(s) 26, 172, 589
Bst4CI ACNGT 1 cut(s) 557
BstDEI CTNAG 1 cut(s) 228
BstF5I GGATG 2 cut(s) 190, 250
BstKTI GATC 3 cut(s) 29, 175, 592
BstMAI GTCTC 1 cut(s) 224
BstMBI GATC 3 cut(s) 26, 172, 589
BstMWI GCNNNNNNNGC 3 cut(s) 128, 155, 354
BstSCI CCNGG 2 cut(s) 352, 417
BstSFI CTRYAG 1 cut(s) 325
BstV1I GCAGC 4 cut(s) 80, 98, 133, 211
BstX2I RGATCY 2 cut(s) 26, 172
BstYI RGATCY 2 cut(s) 26, 172
BsuRI GGCC 3 cut(s) 243, 271, 493
BtrI CACGTC 1 cut(s) 639
BtsCI GGATG 2 cut(s) 190, 250
BtsIMutI CAGTG 1 cut(s) 507
CaiI CAGNNNCTG 1 cut(s) 30
Cfr10I RCCGGY 1 cut(s) 457
Cfr13I GGNCC 1 cut(s) 386
CseI GACGC 1 cut(s) 556
Csp6I GTAC 1 cut(s) 259
CspAI ACCGGT 1 cut(s) 457
CspCI CAANNNNNGTGG 2 cut(s) 201, 236
CviQI GTAC 1 cut(s) 259
DdeI CTNAG 1 cut(s) 228
DpnI GATC 3 cut(s) 28, 174, 591
DpnII GATC 3 cut(s) 26, 172, 589
EciI GGCGGA 1 cut(s) 165
Eco31I GGTCTC 1 cut(s) 224
Eco47I GGWCC 1 cut(s) 386
Eco57I CTGAAG 1 cut(s) 162
EcoO109I RGGNCCY 1 cut(s) 386
FauI CCCGC 1 cut(s) 439
Fnu4HI GCNGC 5 cut(s) 66, 69, 87, 147, 225
FokI GGATG 2 cut(s) 177, 237
Fsp4HI GCNGC 5 cut(s) 66, 69, 87, 147, 225
FspBI CTAG 1 cut(s) 239
GluI GCNGC 5 cut(s) 66, 69, 87, 147, 225
GsuI CTGGAG 1 cut(s) 25
HaeIII GGCC 3 cut(s) 243, 271, 493
HapII CCGG 7 cut(s) 185, 254, 354, 395, 419, 425, 458
HgaI GACGC 1 cut(s) 556
HinfI GANTC 1 cut(s) 107
HpaII CCGG 7 cut(s) 185, 254, 354, 395, 419, 425, 458
HphI GGTGA 2 cut(s) 102, 489
Hpy166II GTNNAC 1 cut(s) 259
Hpy188I TCNGA 1 cut(s) 663
Hpy188III TCNNGA 3 cut(s) 80, 140, 587
Hpy8I GTNNAC 1 cut(s) 259
Hpy99I CGWCG 2 cut(s) 643, 656
HpyAV CCTTC 1 cut(s) 53
HpyCH4III ACNGT 1 cut(s) 557
HpyCH4IV ACGT 1 cut(s) 638
HpyCH4V TGCA 1 cut(s) 86
HpyF10VI GCNNNNNNNGC 3 cut(s) 128, 155, 354
HpyF3I CTNAG 1 cut(s) 228
HpySE526I ACGT 1 cut(s) 638
Kzo9I GATC 3 cut(s) 26, 172, 589
LmnI GCTCC 3 cut(s) 136, 427, 433
Lsp1109I GCAGC 4 cut(s) 80, 98, 133, 211
LweI GCATC 1 cut(s) 73
MaeI CTAG 1 cut(s) 239
MaeII ACGT 1 cut(s) 638
MaeIII GTNAC 2 cut(s) 466, 505
MalI GATC 3 cut(s) 28, 174, 591
MbiI CCGCTC 1 cut(s) 434
MboI GATC 3 cut(s) 26, 172, 589
MflI RGATCY 2 cut(s) 26, 172
MmeI TCCRAC 1 cut(s) 125
MnlI CCTC 8 cut(s) 49, 87, 105, 108, 147, 196, 399, 417
MspI CCGG 7 cut(s) 185, 254, 354, 395, 419, 425, 458
MspR9I CCNGG 2 cut(s) 354, 419
Mva1269I GAATGC 1 cut(s) 670
MwoI GCNNNNNNNGC 3 cut(s) 128, 155, 354
NciI CCSGG 2 cut(s) 354, 419
NdeII GATC 3 cut(s) 26, 172, 589
NlaIV GGNNCC 5 cut(s) 64, 174, 399, 423, 429
NmeAIII GCCGAG 1 cut(s) 184
NmuCI GTSAC 2 cut(s) 466, 505
PctI GAATGC 1 cut(s) 670
PfeI GAWTC 1 cut(s) 107
PinAI ACCGGT 1 cut(s) 457
PkrI GCNGC 5 cut(s) 67, 70, 88, 148, 226
PpuMI RGGWCCY 1 cut(s) 386
Psp5II RGGWCCY 1 cut(s) 386
PspN4I GGNNCC 5 cut(s) 64, 174, 399, 423, 429
PspPI GGNCC 1 cut(s) 386
PspPPI RGGWCCY 1 cut(s) 386
PstNI CAGNNNCTG 1 cut(s) 30
PsuI RGATCY 2 cut(s) 26, 172
RsaI GTAC 1 cut(s) 260
RsaNI GTAC 1 cut(s) 259
SatI GCNGC 5 cut(s) 66, 69, 87, 147, 225
Sau3AI GATC 3 cut(s) 26, 172, 589
Sau96I GGNCC 1 cut(s) 386
ScrFI CCNGG 2 cut(s) 354, 419
SfaNI GCATC 1 cut(s) 73
SfcI CTRYAG 1 cut(s) 325
SinI GGWCC 1 cut(s) 386
SmlI CTYRAG 1 cut(s) 435
SmoI CTYRAG 1 cut(s) 435
SsiI CCGC 3 cut(s) 66, 176, 432
SspMI CTAG 1 cut(s) 239
StyD4I CCNGG 2 cut(s) 352, 417
TaaI ACNGT 1 cut(s) 557
TaiI ACGT 1 cut(s) 641
TaqI TCGA 1 cut(s) 641
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 1 cut(s) 107
TscAI CASTG 1 cut(s) 514
TseFI GTSAC 2 cut(s) 466, 505
TseI GCWGC 4 cut(s) 68, 86, 146, 224
Tsp45I GTSAC 2 cut(s) 466, 505
TspDTI ATGAA 1 cut(s) 281
TspRI CASTG 1 cut(s) 514
VpaK11BI GGWCC 1 cut(s) 386
XspI CTAG 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.