MD16G1161600.v1.1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0015599
Physical Location & Seq
Forward (+)
13188798 .. 13190460
1663 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1161600.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGTGTATAAATAAAATGCCACCTATTAATCCATTGCCCCTCCCTCTCTCCCTCTCTCCCTCCCTCCCTCCACCCTCTTCATTGCCTCTCATGGAGAAACCTCGGGTGACGGAGATTCATGTCCGGATGGACTGTAACGGATGTGTGCAGAAGATCAAGAAAGCGCTACATGGCATCAATGGTATATATGATCTTTACATTGACTTCCCTCAACAAAAATTGACAATAATTGGGTGGGCGGATCCGGAGAAGATAGTAAAGGCGATTAAGAAGACGAGGAAGATTGCCACCATATGTTCCCACACAGAACAACAAACGGAACCTGCTCCACCACCGCCAGAACAAGCGCCGGAGAATGGTTCTCCAGCTCCTGAGGCAGCAAACCCCCCTCCTTCAGAAGCACCACCACCGGCTGAAGCAGCTCCGCCAGCTGAAGTGGCCCCGCCAGCAGAGCCACCAAGAGAATCCCCTCGGCCTGAAAATCCAACACCCGAGCCAACAGTACCAACACCTGTGTCTGCTGAGACCAACCCCGGCCAACAAATACACCACCAGAGACCAAGAGACGTTGGAGAAGCTCATACCATATACCACCACCCACCTGATTATGGCTATAGATATGACTACTCCCAAGGCTACAATGGCTACTGGAACAGATACCATAACAGCCAAGGACCTCCACAGGAGCCTGCCCATATCCCCGCCCCGATGGGACCTCCTCCAATGATCCACGCCCCAATGGACCCTCCTCCAATGAGCCACGCCCCGGGTCCTCCCCCGATGAGCCACGTCCCAATGGGTCCTCCCCCGATGAGCCACTCCCCAATGGTTCCTCCCCCGATGAGCCACGCCCCGATGTGTCCCACCCCACCTCCGCCTATGCATGTGACACACAGCTACAACACATACAGACCATCACCTTATATCACAGAATTTGAATATATCCAGCCACCCCCACAACCCTCACATTTCAGCAGGATGAACCATTACAACGAGCATTTCAGCAGGGCGAACCATTACAACGAGGAGCAGCACGACGTCAACGGCAACGGAAACATCACATCAATGTTTAGCGACGAAAATCCGAATGCGTGTACCGTAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

369

Amino Acids

40.63

Weight (kDa)

6.03

Isoelectric Point (pI)

72.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 41 - 92 2.5e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015599)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g18950
malus_domestica MD13G1161800.v1.1 MD16G1161600.v1.1
prunus_persica Prupe.1G129100_v2.0.a1
pyrus_communis pycom16g13700
rosa_chinensis RchiOBHm_Chr4g0423841
rosa_laevigata RLG00000007527
rosa_multiflora Rmu_sc0003369.1_g000009 Rmu_sc0008198.1_g000001
rosa_roxburghii Rroxscaffold_5G00365960
rosa_rugosa Rorug04G0191200 Rorug04G0191200
rosa_samantha Rh4AG250600 Rh4BG255900 Rh4DG250400
rosa_wichuraiana Rw4G021470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1041
Acc36I ACCTGC 1 cut(s) 331
AccIII TCCGGA 2 cut(s) 123, 244
AciI CCGC 6 cut(s) 239, 335, 425, 443, 702, 875
AclWI GGATC 3 cut(s) 236, 249, 721
AcoI YGGCCR 1 cut(s) 535
AcsI RAATTY 1 cut(s) 932
AcuI CTGAAG 3 cut(s) 378, 435, 453
AcyI GRCGYC 1 cut(s) 1038
AfaI GTAC 2 cut(s) 504, 1096
AfeI AGCGCT 1 cut(s) 165
AfiI CCNNNNNNNGG 3 cut(s) 356, 608, 766
AgsI TTSAA 1 cut(s) 938
AjiI CACGTC 1 cut(s) 790
AluBI AGCT 5 cut(s) 368, 422, 431, 578, 897
AluI AGCT 5 cut(s) 368, 422, 431, 578, 897
Alw26I GTCTC 3 cut(s) 518, 550, 558
AlwI GGATC 3 cut(s) 236, 249, 721
AlwNI CAGNNNCTG 1 cut(s) 371
Ama87I CYCGRG 3 cut(s) 102, 491, 766
Aor13HI TCCGGA 2 cut(s) 123, 244
Aor51HI AGCGCT 1 cut(s) 165
AoxI GGCC 3 cut(s) 438, 473, 535
ApeKI GCWGC 3 cut(s) 377, 419, 1030
ApoI RAATTY 1 cut(s) 932
AseI ATTAAT 1 cut(s) 27
AspLEI GCGC 2 cut(s) 166, 349
AspS9I GGNCC 6 cut(s) 439, 674, 713, 742, 770, 800
AsuC2I CCSGG 3 cut(s) 534, 767, 768
AsuHPI GGTGA 2 cut(s) 118, 909
AvaI CYCGRG 3 cut(s) 102, 491, 766
AvaII GGWCC 5 cut(s) 674, 713, 742, 770, 800
AxyI CCTNAGG 1 cut(s) 372
BamHI GGATCC 1 cut(s) 241
BbsI GAAGAC 1 cut(s) 278
BbvI GCAGC 3 cut(s) 389, 431, 1042
BccI CCATC 3 cut(s) 121, 703, 922
BceAI ACGGC 1 cut(s) 1060
BcnI CCSGG 3 cut(s) 534, 767, 768
BcoDI GTCTC 3 cut(s) 518, 550, 558
BfmI CTRYAG 1 cut(s) 613
BfoI RGCGCY 2 cut(s) 167, 350
BfuAI ACCTGC 1 cut(s) 331
BisI GCNGC 3 cut(s) 378, 420, 1031
BlsI GCNGC 3 cut(s) 379, 421, 1032
Bme1390I CCNGG 3 cut(s) 534, 767, 768
Bme18I GGWCC 5 cut(s) 674, 713, 742, 770, 800
BmeT110I CYCGRG 3 cut(s) 102, 491, 766
BmgBI CACGTC 1 cut(s) 790
BmgT120I GGNCC 6 cut(s) 439, 674, 713, 742, 770, 800
BmiI GGNNCC 9 cut(s) 243, 321, 441, 687, 714, 744, 771, 801, 831
BmrFI CCNGG 3 cut(s) 534, 767, 768
BmsI GCATC 1 cut(s) 183
BpiI GAAGAC 1 cut(s) 278
BpmI CTGGAG 1 cut(s) 348
BpuMI CCSGG 3 cut(s) 534, 767, 768
BsaHI GRCGYC 1 cut(s) 1038
BsaI GGTCTC 2 cut(s) 518, 550
BsaJI CCNNGG 7 cut(s) 101, 470, 532, 631, 670, 765, 766
BsaWI WCCGGW 2 cut(s) 123, 244
BsaXI ACNNNNNCTCC 2 cut(s) 856, 886
Bsc4I CCNNNNNNNGG 3 cut(s) 356, 608, 766
Bse118I RCCGGY 1 cut(s) 409
Bse1I ACTGG 1 cut(s) 653
Bse21I CCTNAGG 1 cut(s) 372
Bse3DI GCAATG 2 cut(s) 32, 80
BseAI TCCGGA 2 cut(s) 123, 244
BseDI CCNNGG 7 cut(s) 101, 470, 532, 631, 670, 765, 766
BseGI GGATG 3 cut(s) 132, 146, 984
BseLI CCNNNNNNNGG 3 cut(s) 356, 608, 766
BseMI GCAATG 2 cut(s) 32, 80
BseMII CTCAG 2 cut(s) 363, 513
BseNI ACTGG 1 cut(s) 653
BseRI GAGGAG 3 cut(s) 708, 738, 1040
BseXI GCAGC 3 cut(s) 389, 431, 1042
BsgI GTGCAG 1 cut(s) 167
BshFI GGCC 3 cut(s) 440, 475, 537
BsiHKCI CYCGRG 3 cut(s) 102, 491, 766
BsiSI CCGG 6 cut(s) 124, 245, 350, 410, 534, 767
BslFI GGGAC 3 cut(s) 726, 776, 846
BslI CCNNNNNNNGG 3 cut(s) 356, 608, 766
BsmAI GTCTC 3 cut(s) 518, 550, 558
BsmBI CGTCTC 1 cut(s) 558
BsmFI GGGAC 3 cut(s) 726, 776, 846
BsmI GAATGC 1 cut(s) 1093
BsnI GGCC 3 cut(s) 440, 475, 537
Bso31I GGTCTC 2 cut(s) 518, 550
BsoBI CYCGRG 3 cut(s) 102, 491, 766
Bsp13I TCCGGA 2 cut(s) 123, 244
Bsp143I GATC 4 cut(s) 153, 190, 241, 726
BspACI CCGC 6 cut(s) 239, 335, 425, 443, 702, 875
BspANI GGCC 3 cut(s) 440, 475, 537
BspCNI CTCAG 2 cut(s) 364, 514
BspEI TCCGGA 2 cut(s) 123, 244
BspLI GGNNCC 9 cut(s) 243, 321, 441, 687, 714, 744, 771, 801, 831
BspMI ACCTGC 1 cut(s) 331
BspPI GGATC 3 cut(s) 236, 249, 721
BspTNI GGTCTC 2 cut(s) 518, 550
BsrDI GCAATG 2 cut(s) 32, 80
BsrFI RCCGGY 1 cut(s) 409
BsrI ACTGG 1 cut(s) 653
BssAI RCCGGY 1 cut(s) 409
BssECI CCNNGG 7 cut(s) 101, 470, 532, 631, 670, 765, 766
BssMI GATC 4 cut(s) 153, 190, 241, 726
BssNI GRCGYC 1 cut(s) 1038
BssT1I CCWWGG 2 cut(s) 631, 670
Bst4CI ACNGT 3 cut(s) 134, 502, 1099
Bst6I CTCTTC 1 cut(s) 82
BstACI GRCGYC 1 cut(s) 1038
BstC8I GCNNGC 3 cut(s) 429, 447, 690
BstDEI CTNAG 2 cut(s) 372, 522
BstF5I GGATG 3 cut(s) 132, 146, 984
BstH2I RGCGCY 2 cut(s) 167, 350
BstHHI GCGC 2 cut(s) 166, 349
BstKTI GATC 4 cut(s) 156, 193, 244, 729
BstMAI GTCTC 3 cut(s) 518, 550, 558
BstMBI GATC 4 cut(s) 153, 190, 241, 726
BstMWI GCNNNNNNNGC 7 cut(s) 374, 419, 428, 437, 446, 451, 642
BstNSI RCATGY 1 cut(s) 887
BstSCI CCNGG 3 cut(s) 532, 765, 766
BstSFI CTRYAG 1 cut(s) 613
BstV1I GCAGC 3 cut(s) 389, 431, 1042
BstV2I GAAGAC 1 cut(s) 278
BstX2I RGATCY 1 cut(s) 241
BstYI RGATCY 1 cut(s) 241
Bsu36I CCTNAGG 1 cut(s) 372
BsuRI GGCC 3 cut(s) 440, 475, 537
BtrI CACGTC 1 cut(s) 790
BtsCI GGATG 3 cut(s) 132, 146, 984
BveI ACCTGC 1 cut(s) 331
Cac8I GCNNGC 3 cut(s) 429, 447, 690
CaiI CAGNNNCTG 1 cut(s) 371
CfoI GCGC 2 cut(s) 166, 349
Cfr10I RCCGGY 1 cut(s) 409
Cfr13I GGNCC 6 cut(s) 439, 674, 713, 742, 770, 800
Cfr9I CCCGGG 1 cut(s) 766
Csp6I GTAC 2 cut(s) 503, 1095
CviAII CATG 4 cut(s) 91, 119, 170, 884
CviQI GTAC 2 cut(s) 503, 1095
DdeI CTNAG 2 cut(s) 372, 522
DpnI GATC 4 cut(s) 155, 192, 243, 728
DpnII GATC 4 cut(s) 153, 190, 241, 726
EaeI YGGCCR 1 cut(s) 535
Eam1104I CTCTTC 1 cut(s) 82
EarI CTCTTC 1 cut(s) 82
EciI GGCGGA 3 cut(s) 254, 414, 864
Eco130I CCWWGG 2 cut(s) 631, 670
Eco31I GGTCTC 2 cut(s) 518, 550
Eco47I GGWCC 5 cut(s) 674, 713, 742, 770, 800
Eco47III AGCGCT 1 cut(s) 165
Eco57I CTGAAG 3 cut(s) 378, 435, 453
Eco81I CCTNAGG 1 cut(s) 372
Eco88I CYCGRG 3 cut(s) 102, 491, 766
EcoO109I RGGNCCY 4 cut(s) 674, 713, 770, 800
EcoT14I CCWWGG 2 cut(s) 631, 670
EcoT22I ATGCAT 1 cut(s) 885
ErhI CCWWGG 2 cut(s) 631, 670
Esp3I CGTCTC 1 cut(s) 558
FaeI CATG 4 cut(s) 94, 122, 173, 887
FaqI GGGAC 3 cut(s) 726, 776, 846
FatI CATG 4 cut(s) 90, 118, 169, 883
FauI CCCGC 2 cut(s) 450, 709
FauNDI CATATG 1 cut(s) 293
Fnu4HI GCNGC 3 cut(s) 378, 420, 1031
FokI GGATG 3 cut(s) 139, 153, 991
Fsp4HI GCNGC 3 cut(s) 378, 420, 1031
GlaI GCGC 2 cut(s) 165, 348
GluI GCNGC 3 cut(s) 378, 420, 1031
GsuI CTGGAG 1 cut(s) 348
HaeII RGCGCY 2 cut(s) 167, 350
HaeIII GGCC 3 cut(s) 440, 475, 537
HapII CCGG 6 cut(s) 124, 245, 350, 410, 534, 767
HhaI GCGC 2 cut(s) 166, 349
Hin1I GRCGYC 1 cut(s) 1038
Hin1II CATG 4 cut(s) 94, 122, 173, 887
Hin6I GCGC 2 cut(s) 164, 347
HinP1I GCGC 2 cut(s) 164, 347
HincII GTYRAC 1 cut(s) 1042
HindII GTYRAC 1 cut(s) 1042
HinfI GANTC 2 cut(s) 115, 464
HpaII CCGG 6 cut(s) 124, 245, 350, 410, 534, 767
HphI GGTGA 2 cut(s) 118, 909
Hpy166II GTNNAC 2 cut(s) 1042, 1095
Hpy188I TCNGA 2 cut(s) 397, 1086
Hpy188III TCNNGA 4 cut(s) 124, 157, 245, 371
Hpy8I GTNNAC 2 cut(s) 1042, 1095
Hpy99I CGWCG 2 cut(s) 1040, 1079
HpyAV CCTTC 1 cut(s) 402
HpyCH4III ACNGT 3 cut(s) 134, 502, 1099
HpyCH4IV ACGT 3 cut(s) 567, 789, 1038
HpyCH4V TGCA 2 cut(s) 148, 883
HpyF10VI GCNNNNNNNGC 7 cut(s) 374, 419, 428, 437, 446, 451, 642
HpyF3I CTNAG 2 cut(s) 372, 522
HpySE526I ACGT 3 cut(s) 567, 789, 1038
Hsp92I GRCGYC 1 cut(s) 1038
Hsp92II CATG 4 cut(s) 94, 122, 173, 887
HspAI GCGC 2 cut(s) 164, 347
Kpn2I TCCGGA 2 cut(s) 123, 244
Kzo9I GATC 4 cut(s) 153, 190, 241, 726
LmnI GCTCC 5 cut(s) 331, 373, 427, 685, 1027
Lsp1109I GCAGC 3 cut(s) 389, 431, 1042
LweI GCATC 1 cut(s) 183
MaeII ACGT 3 cut(s) 567, 789, 1038
MaeIII GTNAC 3 cut(s) 106, 134, 886
MalI GATC 4 cut(s) 155, 192, 243, 728
MboI GATC 4 cut(s) 153, 190, 241, 726
MboII GAAGA 5 cut(s) 69, 163, 262, 283, 292
MflI RGATCY 1 cut(s) 241
MluCI AATT 3 cut(s) 218, 228, 932
MmeI TCCRAC 2 cut(s) 509, 550
Mph1103I ATGCAT 1 cut(s) 885
MroI TCCGGA 2 cut(s) 123, 244
MseI TTAA 2 cut(s) 27, 267
MslI CAYNNNNRTG 1 cut(s) 1064
MspA1I CMGCKG 1 cut(s) 431
MspI CCGG 6 cut(s) 124, 245, 350, 410, 534, 767
MspR9I CCNGG 3 cut(s) 534, 767, 768
Mva1269I GAATGC 1 cut(s) 1093
MwoI GCNNNNNNNGC 7 cut(s) 374, 419, 428, 437, 446, 451, 642
NciI CCSGG 3 cut(s) 534, 767, 768
NdeI CATATG 1 cut(s) 293
NdeII GATC 4 cut(s) 153, 190, 241, 726
NlaIII CATG 4 cut(s) 94, 122, 173, 887
NlaIV GGNNCC 9 cut(s) 243, 321, 441, 687, 714, 744, 771, 801, 831
NmeAIII GCCGAG 1 cut(s) 451
NmuCI GTSAC 2 cut(s) 106, 886
NsiI ATGCAT 1 cut(s) 885
NspI RCATGY 1 cut(s) 887
PctI GAATGC 1 cut(s) 1093
PfeI GAWTC 2 cut(s) 115, 464
PkrI GCNGC 3 cut(s) 379, 421, 1032
PpuMI RGGWCCY 4 cut(s) 674, 713, 770, 800
PshBI ATTAAT 1 cut(s) 27
Psp5II RGGWCCY 4 cut(s) 674, 713, 770, 800
PspN4I GGNNCC 9 cut(s) 243, 321, 441, 687, 714, 744, 771, 801, 831
PspPI GGNCC 6 cut(s) 439, 674, 713, 742, 770, 800
PspPPI RGGWCCY 4 cut(s) 674, 713, 770, 800
PstNI CAGNNNCTG 1 cut(s) 371
PsuI RGATCY 1 cut(s) 241
PvuII CAGCTG 1 cut(s) 431
RsaI GTAC 2 cut(s) 504, 1096
RsaNI GTAC 2 cut(s) 503, 1095
RseI CAYNNNNRTG 1 cut(s) 1064
SaqAI TTAA 2 cut(s) 27, 267
SatI GCNGC 3 cut(s) 378, 420, 1031
Sau3AI GATC 4 cut(s) 153, 190, 241, 726
Sau96I GGNCC 6 cut(s) 439, 674, 713, 742, 770, 800
ScrFI CCNGG 3 cut(s) 534, 767, 768
SfaNI GCATC 1 cut(s) 183
SfcI CTRYAG 1 cut(s) 613
SinI GGWCC 5 cut(s) 674, 713, 742, 770, 800
SmaI CCCGGG 1 cut(s) 768
SmiMI CAYNNNNRTG 1 cut(s) 1064
Sse9I AATT 3 cut(s) 218, 228, 932
SsiI CCGC 6 cut(s) 239, 335, 425, 443, 702, 875
StyD4I CCNGG 3 cut(s) 532, 765, 766
StyI CCWWGG 2 cut(s) 631, 670
TaaI ACNGT 3 cut(s) 134, 502, 1099
TaiI ACGT 3 cut(s) 570, 792, 1041
TasI AATT 3 cut(s) 218, 228, 932
TfiI GAWTC 2 cut(s) 115, 464
Tru1I TTAA 2 cut(s) 27, 267
Tru9I TTAA 2 cut(s) 27, 267
TseFI GTSAC 2 cut(s) 106, 886
TseI GCWGC 3 cut(s) 377, 419, 1030
Tsp45I GTSAC 2 cut(s) 106, 886
TspDTI ATGAA 3 cut(s) 69, 107, 995
TspGWI ACGGA 4 cut(s) 125, 153, 332, 1065
TspMI CCCGGG 1 cut(s) 766
VpaK11BI GGWCC 5 cut(s) 674, 713, 742, 770, 800
VspI ATTAAT 1 cut(s) 27
XapI RAATTY 1 cut(s) 932
XceI RCATGY 1 cut(s) 887
XcmI CCANNNNNNNNNTGG 1 cut(s) 638
XmaI CCCGGG 1 cut(s) 766
ZraI GACGTC 1 cut(s) 1039
Zsp2I ATGCAT 1 cut(s) 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.