Prupe.1G129100_v2.0.a1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0015599
Physical Location & Seq
Forward (+)
10060429 .. 10062215
1787 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G129100.1

Sequence Viewer

Length: 1005 bp
ATGCCCCACATCTTTTGCCTTTCAATTATTCCTTGCCTGTGTACAAATAAAATGCCACCTATTGATTCTTCATTTATACTCTCCTCCACCCCCTCTTCTTCATCACATACTAATCACATTTCCTGGTTTCTTCTCTCCTCTCCATTGCCTCTCATGGCTTCAGGATTAGAGAAACCCCGAGTGACGGAGATACATGTCCGGATGGACTGTAATGGATGCGTACAGAAGATCAAGAAAGCACTACATGGCATCAATGGTATATATGACCTCTACATTGACTTCCCACAACAAAAGCTAACAATAATTGGGTGGGCAGACCCTGAGAAGATAGTCAAAGCCATTAAGAAGACGAGGAAGATTGCCACCATATGTTCCCATACAGAGCAACAAACAGAGCCTGCTCCTCCACCAACAGAACAAGCAGCACCAGAAGGTGGTGCACCAGCTCCAGCTGATCCTCCACCAGCAGAGCAAGCACCACCAGCAGCCGAAGCAGTGCCACCAGCCGAAGGGGCCAAACCAGCAGAGCCACCAAAAGAGTCACCACCACCAGAACTTCCAACACCAGAGGCAGCACCTGTGGCTGCAGAAGCCAACCCAGGCCACCAAATGCACCACCCTCCAAGAGATGTTGGAGAAGTTCATACCATATACCATCATCCACCTGATTATGGATACAGATATGGCTACTCTCAAGGCTACACTGGGTACTGGAACAGATACCATAACAGCCAAGGGCATCCACAGGAGCCTACCCCTACCCCCATCCCAATGGGCCCCACCCCAACACCAACGCCACCAGTCTATGTAACACACAGCTACAACACATACAGGCCATCACCTTATGTCACTGAATATGAGTATATCCAGCCACCCCCTCAACCCACACATCTCAGTAGGATGAATTACTACAATGAGGAACATCACATTAATGTCAGCAATAGCAACGGGAATATCACATCAATTTTTAGCGACGAAAATCCGAATGCATGTGCCGTAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

335

Amino Acids

36.71

Weight (kDa)

5.95

Isoelectric Point (pI)

59.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015599)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g18950
malus_domestica MD13G1161800.v1.1 MD16G1161600.v1.1
prunus_persica Prupe.1G129100_v2.0.a1
pyrus_communis pycom16g13700
rosa_chinensis RchiOBHm_Chr4g0423841
rosa_laevigata RLG00000007527
rosa_multiflora Rmu_sc0003369.1_g000009 Rmu_sc0008198.1_g000001
rosa_roxburghii Rroxscaffold_5G00365960
rosa_rugosa Rorug04G0191200 Rorug04G0191200
rosa_samantha Rh4AG250600 Rh4BG255900 Rh4DG250400
rosa_wichuraiana Rw4G021470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 434
AccIII TCCGGA 1 cut(s) 198
AclWI GGATC 1 cut(s) 449
AcuI CTGAAG 1 cut(s) 144
AfaI GTAC 3 cut(s) 43, 222, 710
AfiI CCNNNNNNNGG 4 cut(s) 184, 434, 509, 671
AflIII ACRYGT 1 cut(s) 193
AgsI TTSAA 1 cut(s) 24
AjnI CCWGG 2 cut(s) 122, 598
AluBI AGCT 4 cut(s) 295, 446, 452, 819
AluI AGCT 4 cut(s) 295, 446, 452, 819
Alw21I GWGCWC 1 cut(s) 442
Alw44I GTGCAC 1 cut(s) 438
AlwI GGATC 1 cut(s) 449
AlwNI CAGNNNCTG 3 cut(s) 320, 398, 578
Ama87I CYCGRG 1 cut(s) 177
Aor13HI TCCGGA 1 cut(s) 198
AoxI GGCC 4 cut(s) 513, 601, 775, 833
ApaI GGGCCC 1 cut(s) 779
ApaLI GTGCAC 1 cut(s) 438
ApeKI GCWGC 4 cut(s) 422, 485, 572, 584
AseI ATTAAT 1 cut(s) 930
AspS9I GGNCC 3 cut(s) 513, 775, 776
AsuHPI GGTGA 2 cut(s) 534, 831
AvaI CYCGRG 1 cut(s) 177
BaeGI GKGCMC 2 cut(s) 442, 779
BanII GRGCYC 1 cut(s) 779
BbsI GAAGAC 1 cut(s) 353
Bbv12I GWGCWC 1 cut(s) 442
BbvI GCAGC 4 cut(s) 434, 497, 571, 584
BccI CCATC 4 cut(s) 196, 663, 773, 844
BceAI ACGGC 1 cut(s) 980
BciT130I CCWGG 2 cut(s) 124, 600
BciVI GTATCC 1 cut(s) 668
BfmI CTRYAG 1 cut(s) 585
BfuI GTATCC 1 cut(s) 668
BglI GCCNNNNNGGC 1 cut(s) 512
BisI GCNGC 4 cut(s) 423, 486, 573, 585
BlsI GCNGC 4 cut(s) 424, 487, 574, 586
Bme1390I CCNGG 2 cut(s) 124, 600
BmeT110I CYCGRG 1 cut(s) 177
BmgT120I GGNCC 3 cut(s) 513, 775, 776
BmiI GGNNCC 4 cut(s) 514, 750, 777, 778
BmrFI CCNGG 2 cut(s) 124, 600
BmrI ACTGGG 1 cut(s) 714
BmsI GCATC 3 cut(s) 206, 258, 748
BmuI ACTGGG 1 cut(s) 714
BpiI GAAGAC 1 cut(s) 353
BpmI CTGGAG 1 cut(s) 432
BpuEI CTTGAG 1 cut(s) 678
BsaJI CCNNGG 2 cut(s) 598, 733
BsaWI WCCGGW 1 cut(s) 198
Bsc4I CCNNNNNNNGG 4 cut(s) 184, 434, 509, 671
Bse1I ACTGG 3 cut(s) 709, 716, 800
Bse3DI GCAATG 1 cut(s) 143
BseAI TCCGGA 1 cut(s) 198
BseBI CCWGG 2 cut(s) 124, 600
BseDI CCNNGG 2 cut(s) 598, 733
BseGI GGATG 6 cut(s) 207, 221, 658, 739, 765, 906
BseLI CCNNNNNNNGG 4 cut(s) 184, 434, 509, 671
BseMI GCAATG 1 cut(s) 143
BseMII CTCAG 2 cut(s) 312, 907
BseNI ACTGG 3 cut(s) 709, 716, 800
BseRI GAGGAG 3 cut(s) 73, 127, 393
BseSI GKGCMC 2 cut(s) 442, 779
BseXI GCAGC 4 cut(s) 434, 497, 571, 584
BshFI GGCC 4 cut(s) 515, 603, 777, 835
BsiHKAI GWGCWC 1 cut(s) 442
BsiHKCI CYCGRG 1 cut(s) 177
BsiSI CCGG 1 cut(s) 199
BslI CCNNNNNNNGG 4 cut(s) 184, 434, 509, 671
BsmI GAATGC 1 cut(s) 991
BsnI GGCC 4 cut(s) 515, 603, 777, 835
BsoBI CYCGRG 1 cut(s) 177
Bsp120I GGGCCC 1 cut(s) 775
Bsp1286I GDGCHC 2 cut(s) 442, 779
Bsp13I TCCGGA 1 cut(s) 198
Bsp1407I TGTACA 1 cut(s) 41
Bsp143I GATC 2 cut(s) 228, 454
BspANI GGCC 4 cut(s) 515, 603, 777, 835
BspCNI CTCAG 2 cut(s) 313, 906
BspEI TCCGGA 1 cut(s) 198
BspLI GGNNCC 4 cut(s) 514, 750, 777, 778
BspMAI CTGCAG 1 cut(s) 589
BspPI GGATC 1 cut(s) 449
BsrDI GCAATG 1 cut(s) 143
BsrGI TGTACA 1 cut(s) 41
BsrI ACTGG 3 cut(s) 709, 716, 800
BssECI CCNNGG 2 cut(s) 598, 733
BssMI GATC 2 cut(s) 228, 454
BssT1I CCWWGG 1 cut(s) 733
Bst2UI CCWGG 2 cut(s) 124, 600
Bst4CI ACNGT 1 cut(s) 209
Bst6I CTCTTC 1 cut(s) 100
BstAUI TGTACA 1 cut(s) 41
BstC8I GCNNGC 2 cut(s) 399, 474
BstDEI CTNAG 2 cut(s) 321, 893
BstF5I GGATG 6 cut(s) 207, 221, 658, 739, 765, 906
BstKTI GATC 2 cut(s) 231, 457
BstMBI GATC 2 cut(s) 228, 454
BstMWI GCNNNNNNNGC 7 cut(s) 473, 482, 491, 512, 521, 581, 590
BstNI CCWGG 2 cut(s) 124, 600
BstNSI RCATGY 2 cut(s) 197, 993
BstSCI CCNGG 2 cut(s) 122, 598
BstSFI CTRYAG 1 cut(s) 585
BstSLI GKGCMC 2 cut(s) 442, 779
BstV1I GCAGC 4 cut(s) 434, 497, 571, 584
BstV2I GAAGAC 1 cut(s) 353
BstXI CCANNNNNNTGG 1 cut(s) 772
BsuI GTATCC 1 cut(s) 668
BsuRI GGCC 4 cut(s) 515, 603, 777, 835
BtsCI GGATG 6 cut(s) 207, 221, 658, 739, 765, 906
BtsI GCAGTG 1 cut(s) 501
BtsIMutI CAGTG 3 cut(s) 501, 702, 849
Cac8I GCNNGC 2 cut(s) 399, 474
CaiI CAGNNNCTG 3 cut(s) 320, 398, 578
Cfr13I GGNCC 3 cut(s) 513, 775, 776
Csp6I GTAC 3 cut(s) 42, 221, 709
CspCI CAANNNNNGTGG 1 cut(s) 31
CviAII CATG 4 cut(s) 154, 194, 245, 990
CviQI GTAC 3 cut(s) 42, 221, 709
DdeI CTNAG 2 cut(s) 321, 893
DpnI GATC 2 cut(s) 230, 456
DpnII GATC 2 cut(s) 228, 454
Eam1104I CTCTTC 1 cut(s) 100
EarI CTCTTC 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 733
Eco24I GRGCYC 1 cut(s) 779
Eco57I CTGAAG 1 cut(s) 144
Eco88I CYCGRG 1 cut(s) 177
EcoO109I RGGNCCY 1 cut(s) 776
EcoRII CCWGG 2 cut(s) 122, 598
EcoT14I CCWWGG 1 cut(s) 733
EcoT22I ATGCAT 1 cut(s) 991
EcoT38I GRGCYC 1 cut(s) 779
ErhI CCWWGG 1 cut(s) 733
FaeI CATG 4 cut(s) 157, 197, 248, 993
FatI CATG 4 cut(s) 153, 193, 244, 989
FauNDI CATATG 1 cut(s) 368
Fnu4HI GCNGC 4 cut(s) 423, 486, 573, 585
FokI GGATG 6 cut(s) 214, 228, 645, 726, 752, 913
FriOI GRGCYC 1 cut(s) 779
Fsp4HI GCNGC 4 cut(s) 423, 486, 573, 585
GluI GCNGC 4 cut(s) 423, 486, 573, 585
GsuI CTGGAG 1 cut(s) 432
HaeIII GGCC 4 cut(s) 515, 603, 777, 835
HapII CCGG 1 cut(s) 199
Hin1II CATG 4 cut(s) 157, 197, 248, 993
HinfI GANTC 2 cut(s) 65, 539
HpaII CCGG 1 cut(s) 199
HphI GGTGA 2 cut(s) 534, 831
Hpy166II GTNNAC 2 cut(s) 42, 440
Hpy188I TCNGA 1 cut(s) 984
Hpy188III TCNNGA 3 cut(s) 162, 199, 232
Hpy8I GTNNAC 2 cut(s) 42, 440
Hpy99I CGWCG 1 cut(s) 977
HpyAV CCTTC 2 cut(s) 425, 503
HpyCH4III ACNGT 1 cut(s) 209
HpyCH4V TGCA 4 cut(s) 440, 587, 613, 989
HpyF10VI GCNNNNNNNGC 7 cut(s) 473, 482, 491, 512, 521, 581, 590
HpyF3I CTNAG 2 cut(s) 321, 893
Hsp92II CATG 4 cut(s) 157, 197, 248, 993
Kpn2I TCCGGA 1 cut(s) 198
Kzo9I GATC 2 cut(s) 228, 454
LmnI GCTCC 3 cut(s) 406, 451, 748
Lsp1109I GCAGC 4 cut(s) 434, 497, 571, 584
LweI GCATC 3 cut(s) 206, 258, 748
MaeIII GTNAC 4 cut(s) 181, 540, 808, 847
MalI GATC 2 cut(s) 230, 456
MboI GATC 2 cut(s) 228, 454
MboII GAAGA 8 cut(s) 60, 87, 90, 122, 238, 337, 358, 367
MhlI GDGCHC 2 cut(s) 442, 779
MluCI AATT 4 cut(s) 24, 303, 904, 963
MlyI GAGTC 1 cut(s) 548
MmeI TCCRAC 2 cut(s) 584, 613
Mph1103I ATGCAT 1 cut(s) 991
MroI TCCGGA 1 cut(s) 198
MseI TTAA 2 cut(s) 342, 930
MslI CAYNNNNRTG 2 cut(s) 770, 930
MspA1I CMGCKG 1 cut(s) 452
MspI CCGG 1 cut(s) 199
MspR9I CCNGG 2 cut(s) 124, 600
Mva1269I GAATGC 1 cut(s) 991
MvaI CCWGG 2 cut(s) 124, 600
MwoI GCNNNNNNNGC 7 cut(s) 473, 482, 491, 512, 521, 581, 590
NdeI CATATG 1 cut(s) 368
NdeII GATC 2 cut(s) 228, 454
NlaIII CATG 4 cut(s) 157, 197, 248, 993
NlaIV GGNNCC 4 cut(s) 514, 750, 777, 778
NmuCI GTSAC 3 cut(s) 181, 540, 847
NsiI ATGCAT 1 cut(s) 991
NspI RCATGY 2 cut(s) 197, 993
PciI ACATGT 1 cut(s) 193
PctI GAATGC 1 cut(s) 991
PfeI GAWTC 1 cut(s) 65
PflMI CCANNNNNTGG 1 cut(s) 434
PkrI GCNGC 4 cut(s) 424, 487, 574, 586
PleI GAGTC 1 cut(s) 547
PpsI GAGTC 1 cut(s) 547
PscI ACATGT 1 cut(s) 193
PshBI ATTAAT 1 cut(s) 930
Psp6I CCWGG 2 cut(s) 122, 598
PspGI CCWGG 2 cut(s) 122, 598
PspN4I GGNNCC 4 cut(s) 514, 750, 777, 778
PspOMI GGGCCC 1 cut(s) 775
PspPI GGNCC 3 cut(s) 513, 775, 776
PstI CTGCAG 1 cut(s) 589
PstNI CAGNNNCTG 3 cut(s) 320, 398, 578
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 3 cut(s) 43, 222, 710
RsaNI GTAC 3 cut(s) 42, 221, 709
RseI CAYNNNNRTG 2 cut(s) 770, 930
SaqAI TTAA 2 cut(s) 342, 930
SatI GCNGC 4 cut(s) 423, 486, 573, 585
Sau3AI GATC 2 cut(s) 228, 454
Sau96I GGNCC 3 cut(s) 513, 775, 776
SchI GAGTC 1 cut(s) 548
ScrFI CCNGG 2 cut(s) 124, 600
SduI GDGCHC 2 cut(s) 442, 779
SfaNI GCATC 3 cut(s) 206, 258, 748
SfcI CTRYAG 1 cut(s) 585
SmiMI CAYNNNNRTG 2 cut(s) 770, 930
SmlI CTYRAG 1 cut(s) 693
SmoI CTYRAG 1 cut(s) 693
Sse9I AATT 4 cut(s) 24, 303, 904, 963
StyD4I CCNGG 2 cut(s) 122, 598
StyI CCWWGG 1 cut(s) 733
TaaI ACNGT 1 cut(s) 209
TasI AATT 4 cut(s) 24, 303, 904, 963
TatI WGTACW 1 cut(s) 41
TfiI GAWTC 1 cut(s) 65
Tru1I TTAA 2 cut(s) 342, 930
Tru9I TTAA 2 cut(s) 342, 930
TscAI CASTG 3 cut(s) 501, 709, 856
TseFI GTSAC 3 cut(s) 181, 540, 847
TseI GCWGC 4 cut(s) 422, 485, 572, 584
Tsp45I GTSAC 3 cut(s) 181, 540, 847
TspDTI ATGAA 4 cut(s) 60, 90, 632, 917
TspGWI ACGGA 1 cut(s) 200
TspRI CASTG 3 cut(s) 501, 709, 856
Van91I CCANNNNNTGG 1 cut(s) 434
VneI GTGCAC 1 cut(s) 438
VspI ATTAAT 1 cut(s) 930
XceI RCATGY 2 cut(s) 197, 993
Zsp2I ATGCAT 1 cut(s) 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.