pycom16g13700

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0015599
Physical Location & Seq
Forward (+)
9777260 .. 9778350
1091 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g13700.1

Sequence Viewer

Length: 963 bp
ATGCAGAAACCTCGGGTGACGGAGATTCATGTCCGGATGGATTGTAACGGATGCGTGCAGAAGATCAAGAAAGCGCTACATGGCATCAATGGTATATATGATCTTTACATTGACTTCCCTCAACAAAAGTTGACAATAATTGGGTGGGCGGATCCGGAGAAGATAGTAAAAGCGATTAAGAAGACGAGGAAGATTGCCACCATATGTTCCCACACAGAACAACAAACGGAACCTGCTCCACCACCGCCAGAACAAGCGCCGGAGAATGGTTCTCCAGCTCCTGAGGCAGCAAACCCCCCTCCTTCAGAAGCACCACCACCGGCTGAAGCAGCTCCGCCGGCTGAAGTGGCCCCGCCGCATGAAAATCCAACACCCGAGCCAACAGGACCAACACTTGTGTCTGCTGAGACCAACCCCGGCCAACAAATACACCACCAGAGACCAAGAGACGTTGGAGAAGTTCATACCATATACCACCACCCACCTGATTATGGCTACAGATATGGCTACTCCCAAGGCTACAATGGCTACTGGAACAGATACCATAACAGCCAAGGACCTCCACAGGAGCCTGCCCATACCCCCGCCCCGATGGGACCTCCTCCAATGAGCCACTCCCCAATGGACCCTCCTCCAATGAGCCACGCCCCAATGGGTCCTCCCCCGATGAGCCACGCCCCAATGGGTCCTCCCCCTATGAGCCACGCCCCGATGTGTGCAACCCCACCTCCGCCTATGCATGTGACACACAGCTACAACACATACAGACCATCACCTTATATCACAGAATTTGAATATATCCAGCCACCCCCACAACCCTCACATTTCAGCAGGACGAACCATTACAACGAGCATTTCAGCAGGGCGAACCATTACAACGAGGAGCAGCACTACGTCAACGGCAACGGAAACATCACATCAATGTTTAGTGACGAAAATCCGAATGCGTGTACCGTAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

321

Amino Acids

35.46

Weight (kDa)

6.17

Isoelectric Point (pI)

60.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 11 - 62 2.1e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015599)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g18950
malus_domestica MD13G1161800.v1.1 MD16G1161600.v1.1
prunus_persica Prupe.1G129100_v2.0.a1
pyrus_communis pycom16g13700
rosa_chinensis RchiOBHm_Chr4g0423841
rosa_laevigata RLG00000007527
rosa_multiflora Rmu_sc0003369.1_g000009 Rmu_sc0008198.1_g000001
rosa_roxburghii Rroxscaffold_5G00365960
rosa_rugosa Rorug04G0191200 Rorug04G0191200
rosa_samantha Rh4AG250600 Rh4BG255900 Rh4DG250400
rosa_wichuraiana Rw4G021470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 241
AccIII TCCGGA 2 cut(s) 33, 154
AciI CCGC 7 cut(s) 149, 245, 335, 353, 356, 585, 731
AclWI GGATC 2 cut(s) 146, 159
AcoI YGGCCR 1 cut(s) 418
AcsI RAATTY 1 cut(s) 788
AcuI CTGAAG 3 cut(s) 288, 345, 363
AfaI GTAC 1 cut(s) 952
AfeI AGCGCT 1 cut(s) 75
AfiI CCNNNNNNNGG 2 cut(s) 266, 491
AgsI TTSAA 1 cut(s) 794
AluBI AGCT 3 cut(s) 278, 332, 753
AluI AGCT 3 cut(s) 278, 332, 753
Alw26I GTCTC 3 cut(s) 401, 433, 441
AlwI GGATC 2 cut(s) 146, 159
AlwNI CAGNNNCTG 1 cut(s) 281
Ama87I CYCGRG 2 cut(s) 12, 374
Aor13HI TCCGGA 2 cut(s) 33, 154
Aor51HI AGCGCT 1 cut(s) 75
AoxI GGCC 2 cut(s) 348, 418
ApeKI GCWGC 3 cut(s) 287, 329, 886
ApoI RAATTY 1 cut(s) 788
AspLEI GCGC 2 cut(s) 76, 259
AspS9I GGNCC 7 cut(s) 349, 386, 557, 596, 625, 656, 686
AsuC2I CCSGG 1 cut(s) 417
AsuHPI GGTGA 2 cut(s) 28, 765
AvaI CYCGRG 2 cut(s) 12, 374
AvaII GGWCC 6 cut(s) 386, 557, 596, 625, 656, 686
AxyI CCTNAGG 1 cut(s) 282
BamHI GGATCC 1 cut(s) 151
BbsI GAAGAC 1 cut(s) 188
BbvI GCAGC 3 cut(s) 299, 341, 898
BccI CCATC 3 cut(s) 31, 586, 778
BceAI ACGGC 1 cut(s) 916
BcgI CGANNNNNNTGC 1 cut(s) 27
BcnI CCSGG 1 cut(s) 417
BcoDI GTCTC 3 cut(s) 401, 433, 441
BfmI CTRYAG 1 cut(s) 496
BfoI RGCGCY 2 cut(s) 77, 260
BfuAI ACCTGC 1 cut(s) 241
BisI GCNGC 4 cut(s) 288, 330, 356, 887
BlsI GCNGC 4 cut(s) 289, 331, 357, 888
Bme1390I CCNGG 1 cut(s) 417
Bme18I GGWCC 6 cut(s) 386, 557, 596, 625, 656, 686
BmeT110I CYCGRG 2 cut(s) 12, 374
BmgT120I GGNCC 7 cut(s) 349, 386, 557, 596, 625, 656, 686
BmiI GGNNCC 8 cut(s) 153, 231, 351, 570, 597, 627, 657, 687
BmrFI CCNGG 1 cut(s) 417
BmsI GCATC 2 cut(s) 41, 93
BpiI GAAGAC 1 cut(s) 188
BpmI CTGGAG 1 cut(s) 258
BpuMI CCSGG 1 cut(s) 417
BsaI GGTCTC 2 cut(s) 401, 433
BsaJI CCNNGG 4 cut(s) 11, 415, 514, 553
BsaWI WCCGGW 2 cut(s) 33, 154
BsaXI ACNNNNNCTCC 2 cut(s) 712, 742
Bsc4I CCNNNNNNNGG 2 cut(s) 266, 491
Bse118I RCCGGY 2 cut(s) 319, 337
Bse1I ACTGG 1 cut(s) 536
Bse21I CCTNAGG 1 cut(s) 282
BseAI TCCGGA 2 cut(s) 33, 154
BseDI CCNNGG 4 cut(s) 11, 415, 514, 553
BseGI GGATG 2 cut(s) 42, 56
BseLI CCNNNNNNNGG 2 cut(s) 266, 491
BseMII CTCAG 2 cut(s) 273, 396
BseNI ACTGG 1 cut(s) 536
BseRI GAGGAG 3 cut(s) 591, 621, 896
BseXI GCAGC 3 cut(s) 299, 341, 898
BsgI GTGCAG 1 cut(s) 77
BshFI GGCC 2 cut(s) 350, 420
BsiHKCI CYCGRG 2 cut(s) 12, 374
BsiSI CCGG 6 cut(s) 34, 155, 260, 320, 338, 417
BslFI GGGAC 1 cut(s) 609
BslI CCNNNNNNNGG 2 cut(s) 266, 491
BsmAI GTCTC 3 cut(s) 401, 433, 441
BsmBI CGTCTC 1 cut(s) 441
BsmFI GGGAC 1 cut(s) 609
BsmI GAATGC 1 cut(s) 949
BsnI GGCC 2 cut(s) 350, 420
Bso31I GGTCTC 2 cut(s) 401, 433
BsoBI CYCGRG 2 cut(s) 12, 374
Bsp13I TCCGGA 2 cut(s) 33, 154
Bsp143I GATC 3 cut(s) 63, 100, 151
BspACI CCGC 7 cut(s) 149, 245, 335, 353, 356, 585, 731
BspANI GGCC 2 cut(s) 350, 420
BspCNI CTCAG 2 cut(s) 274, 397
BspEI TCCGGA 2 cut(s) 33, 154
BspLI GGNNCC 8 cut(s) 153, 231, 351, 570, 597, 627, 657, 687
BspMI ACCTGC 1 cut(s) 241
BspPI GGATC 2 cut(s) 146, 159
BspTNI GGTCTC 2 cut(s) 401, 433
BsrFI RCCGGY 2 cut(s) 319, 337
BsrI ACTGG 1 cut(s) 536
BssAI RCCGGY 2 cut(s) 319, 337
BssECI CCNNGG 4 cut(s) 11, 415, 514, 553
BssMI GATC 3 cut(s) 63, 100, 151
BssT1I CCWWGG 2 cut(s) 514, 553
Bst4CI ACNGT 1 cut(s) 955
BstC8I GCNNGC 3 cut(s) 56, 339, 573
BstDEI CTNAG 2 cut(s) 282, 405
BstF5I GGATG 2 cut(s) 42, 56
BstH2I RGCGCY 2 cut(s) 77, 260
BstHHI GCGC 2 cut(s) 76, 259
BstKTI GATC 3 cut(s) 66, 103, 154
BstMAI GTCTC 3 cut(s) 401, 433, 441
BstMBI GATC 3 cut(s) 63, 100, 151
BstMWI GCNNNNNNNGC 5 cut(s) 284, 329, 338, 347, 525
BstNSI RCATGY 1 cut(s) 743
BstSCI CCNGG 1 cut(s) 415
BstSFI CTRYAG 1 cut(s) 496
BstV1I GCAGC 3 cut(s) 299, 341, 898
BstV2I GAAGAC 1 cut(s) 188
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
Bsu36I CCTNAGG 1 cut(s) 282
BsuRI GGCC 2 cut(s) 350, 420
BtsCI GGATG 2 cut(s) 42, 56
BveI ACCTGC 1 cut(s) 241
Cac8I GCNNGC 3 cut(s) 56, 339, 573
CaiI CAGNNNCTG 1 cut(s) 281
CfoI GCGC 2 cut(s) 76, 259
Cfr10I RCCGGY 2 cut(s) 319, 337
Cfr13I GGNCC 7 cut(s) 349, 386, 557, 596, 625, 656, 686
Csp6I GTAC 1 cut(s) 951
CviAII CATG 4 cut(s) 29, 80, 359, 740
CviQI GTAC 1 cut(s) 951
DdeI CTNAG 2 cut(s) 282, 405
DpnI GATC 3 cut(s) 65, 102, 153
DpnII GATC 3 cut(s) 63, 100, 151
EaeI YGGCCR 1 cut(s) 418
EciI GGCGGA 3 cut(s) 164, 324, 720
Eco130I CCWWGG 2 cut(s) 514, 553
Eco31I GGTCTC 2 cut(s) 401, 433
Eco47I GGWCC 6 cut(s) 386, 557, 596, 625, 656, 686
Eco47III AGCGCT 1 cut(s) 75
Eco57I CTGAAG 3 cut(s) 288, 345, 363
Eco81I CCTNAGG 1 cut(s) 282
Eco88I CYCGRG 2 cut(s) 12, 374
EcoO109I RGGNCCY 4 cut(s) 557, 596, 656, 686
EcoT14I CCWWGG 2 cut(s) 514, 553
EcoT22I ATGCAT 1 cut(s) 741
ErhI CCWWGG 2 cut(s) 514, 553
Esp3I CGTCTC 1 cut(s) 441
FaeI CATG 4 cut(s) 32, 83, 362, 743
FaqI GGGAC 1 cut(s) 609
FatI CATG 4 cut(s) 28, 79, 358, 739
FauI CCCGC 2 cut(s) 360, 592
FauNDI CATATG 1 cut(s) 203
Fnu4HI GCNGC 4 cut(s) 288, 330, 356, 887
FokI GGATG 2 cut(s) 49, 63
Fsp4HI GCNGC 4 cut(s) 288, 330, 356, 887
GlaI GCGC 2 cut(s) 75, 258
GluI GCNGC 4 cut(s) 288, 330, 356, 887
GsuI CTGGAG 1 cut(s) 258
HaeII RGCGCY 2 cut(s) 77, 260
HaeIII GGCC 2 cut(s) 350, 420
HapII CCGG 6 cut(s) 34, 155, 260, 320, 338, 417
HhaI GCGC 2 cut(s) 76, 259
Hin1II CATG 4 cut(s) 32, 83, 362, 743
Hin6I GCGC 2 cut(s) 74, 257
HinP1I GCGC 2 cut(s) 74, 257
HincII GTYRAC 2 cut(s) 132, 898
HindII GTYRAC 2 cut(s) 132, 898
HinfI GANTC 1 cut(s) 25
HpaII CCGG 6 cut(s) 34, 155, 260, 320, 338, 417
HphI GGTGA 2 cut(s) 28, 765
Hpy166II GTNNAC 3 cut(s) 132, 898, 951
Hpy188I TCNGA 2 cut(s) 307, 942
Hpy188III TCNNGA 4 cut(s) 34, 67, 155, 281
Hpy8I GTNNAC 3 cut(s) 132, 898, 951
HpyAV CCTTC 1 cut(s) 312
HpyCH4III ACNGT 1 cut(s) 955
HpyCH4IV ACGT 2 cut(s) 450, 894
HpyCH4V TGCA 4 cut(s) 4, 58, 719, 739
HpyF10VI GCNNNNNNNGC 5 cut(s) 284, 329, 338, 347, 525
HpyF3I CTNAG 2 cut(s) 282, 405
HpySE526I ACGT 2 cut(s) 450, 894
Hsp92II CATG 4 cut(s) 32, 83, 362, 743
HspAI GCGC 2 cut(s) 74, 257
Kpn2I TCCGGA 2 cut(s) 33, 154
KroI GCCGGC 1 cut(s) 337
KroNI GCCGGC 1 cut(s) 339
Kzo9I GATC 3 cut(s) 63, 100, 151
LmnI GCTCC 5 cut(s) 241, 283, 337, 568, 883
Lsp1109I GCAGC 3 cut(s) 299, 341, 898
LweI GCATC 2 cut(s) 41, 93
MaeII ACGT 2 cut(s) 450, 894
MaeIII GTNAC 4 cut(s) 16, 44, 742, 929
MalI GATC 3 cut(s) 65, 102, 153
MboI GATC 3 cut(s) 63, 100, 151
MboII GAAGA 4 cut(s) 73, 172, 193, 202
MflI RGATCY 1 cut(s) 151
MluCI AATT 2 cut(s) 138, 788
MmeI TCCRAC 2 cut(s) 392, 433
Mph1103I ATGCAT 1 cut(s) 741
MroI TCCGGA 2 cut(s) 33, 154
MroNI GCCGGC 1 cut(s) 337
MseI TTAA 1 cut(s) 177
MslI CAYNNNNRTG 1 cut(s) 920
MspI CCGG 6 cut(s) 34, 155, 260, 320, 338, 417
MspR9I CCNGG 1 cut(s) 417
Mva1269I GAATGC 1 cut(s) 949
MwoI GCNNNNNNNGC 5 cut(s) 284, 329, 338, 347, 525
NaeI GCCGGC 1 cut(s) 339
NciI CCSGG 1 cut(s) 417
NdeI CATATG 1 cut(s) 203
NdeII GATC 3 cut(s) 63, 100, 151
NgoMIV GCCGGC 1 cut(s) 337
NlaIII CATG 4 cut(s) 32, 83, 362, 743
NlaIV GGNNCC 8 cut(s) 153, 231, 351, 570, 597, 627, 657, 687
NmuCI GTSAC 3 cut(s) 16, 742, 929
NsiI ATGCAT 1 cut(s) 741
NspI RCATGY 1 cut(s) 743
PctI GAATGC 1 cut(s) 949
PdiI GCCGGC 1 cut(s) 339
PfeI GAWTC 1 cut(s) 25
PkrI GCNGC 4 cut(s) 289, 331, 357, 888
PpuMI RGGWCCY 4 cut(s) 557, 596, 656, 686
Psp5II RGGWCCY 4 cut(s) 557, 596, 656, 686
PspN4I GGNNCC 8 cut(s) 153, 231, 351, 570, 597, 627, 657, 687
PspPI GGNCC 7 cut(s) 349, 386, 557, 596, 625, 656, 686
PspPPI RGGWCCY 4 cut(s) 557, 596, 656, 686
PstNI CAGNNNCTG 1 cut(s) 281
PsuI RGATCY 1 cut(s) 151
RsaI GTAC 1 cut(s) 952
RsaNI GTAC 1 cut(s) 951
RseI CAYNNNNRTG 1 cut(s) 920
SaqAI TTAA 1 cut(s) 177
SatI GCNGC 4 cut(s) 288, 330, 356, 887
Sau3AI GATC 3 cut(s) 63, 100, 151
Sau96I GGNCC 7 cut(s) 349, 386, 557, 596, 625, 656, 686
ScrFI CCNGG 1 cut(s) 417
SfaNI GCATC 2 cut(s) 41, 93
SfcI CTRYAG 1 cut(s) 496
SinI GGWCC 6 cut(s) 386, 557, 596, 625, 656, 686
SmiMI CAYNNNNRTG 1 cut(s) 920
Sse9I AATT 2 cut(s) 138, 788
SsiI CCGC 7 cut(s) 149, 245, 335, 353, 356, 585, 731
StyD4I CCNGG 1 cut(s) 415
StyI CCWWGG 2 cut(s) 514, 553
TaaI ACNGT 1 cut(s) 955
TaiI ACGT 2 cut(s) 453, 897
TasI AATT 2 cut(s) 138, 788
TauI GCSGC 1 cut(s) 358
TfiI GAWTC 1 cut(s) 25
Tru1I TTAA 1 cut(s) 177
Tru9I TTAA 1 cut(s) 177
TseFI GTSAC 3 cut(s) 16, 742, 929
TseI GCWGC 3 cut(s) 287, 329, 886
Tsp45I GTSAC 3 cut(s) 16, 742, 929
TspDTI ATGAA 3 cut(s) 17, 375, 452
TspGWI ACGGA 4 cut(s) 35, 63, 242, 921
VpaK11BI GGWCC 6 cut(s) 386, 557, 596, 625, 656, 686
XapI RAATTY 1 cut(s) 788
XceI RCATGY 1 cut(s) 743
XcmI CCANNNNNNNNNTGG 1 cut(s) 521
Zsp2I ATGCAT 1 cut(s) 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.