RchiOBHm_Chr4g0423841

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0015599
Physical Location & Seq
Reverse (-)
49447334 .. 49448565
1232 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39317

Sequence Viewer

Length: 933 bp
ATGGTCCCAGAATTAGAGAAACCTCGGATTACCGAGATACATGTTCGGATGGATTGTAATGGGTGTGTACAGAAGATTAAGAAAGCACTACATGGCATCAACGGTATATATGATCTCTACATTGACTTCCCTCAGCAAAAGTTAACAATAATAGGGTGGGCGGATCCAGAGAAGATAGTAAAAGCCATTAAAAAGACGAGGAAGATTGCCACCATATGTTCCCATATAGAACAACCAACAGAGCCTGCTTCTCCTCCAGCAGAACAACCCCCCGAAGGTGCCGCACCACCACCTCCCGATGCAGCAAACCCTCCTCCAACAGAATCACCTCATCCACTAGCCGAACCAGCTCCACCTCCTGAAGCTGCCCCACCAGCCGAGCCACCAAAGGATCCGCCACCACCGGAACATCCACAACCAGAGGCACTACCAACACCTGTGGCTGCTGAGACCAATCTAGGCCAACAACATCCGGTGTACCACCACAGGCCAAGAGAAGTTGGGGAAGTTCATACCATATACCACCACCCACCTGATTATGGCTACAGATACGGCTACCCTAATGGCTACCCCGGCTACTATAACAGATACCATAATAGTCAAGGACCTCCACCGGAACCAACTCCTCCCCCCGCCCCGGCTCCGGCTCCGGCCCCGGCTCCAGCTCCGGCTCCGGCTCCAGCTCAGGTTCCAGTCCATACACCACCGGTCTATGTGACACATAGCTACAACACATACAGGCCATCACCTTATGTCACTGAATATGAGTATGTTCAGCCACCCCCACCACAACAGACGCATTACAGTAGGATCAGCCAATACCACCACTATAATGAGGATCAACCGTATCACAATATCAGCAATGGAAATGGAAATGGCAACATCACGTCGATTTTTAGCGACGAAAATCCGAATGCGTGTGCTGTAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

310

Amino Acids

34.12

Weight (kDa)

5.87

Isoelectric Point (pI)

70.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 15 - 66 2e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015599)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g18950
malus_domestica MD13G1161800.v1.1 MD16G1161600.v1.1
prunus_persica Prupe.1G129100_v2.0.a1
pyrus_communis pycom16g13700
rosa_chinensis RchiOBHm_Chr4g0423841
rosa_laevigata RLG00000007527
rosa_multiflora Rmu_sc0003369.1_g000009 Rmu_sc0008198.1_g000001
rosa_roxburghii Rroxscaffold_5G00365960
rosa_rugosa Rorug04G0191200 Rorug04G0191200
rosa_samantha Rh4AG250600 Rh4BG255900 Rh4DG250400
rosa_wichuraiana Rw4G021470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 278
AciI CCGC 4 cut(s) 161, 282, 395, 633
AclWI GGATC 6 cut(s) 158, 171, 386, 399, 818, 846
AcuI CTGAAG 1 cut(s) 381
AfaI GTAC 2 cut(s) 69, 479
AfiI CCNNNNNNNGG 4 cut(s) 275, 539, 637, 643
AflIII ACRYGT 1 cut(s) 40
AgeI ACCGGT 1 cut(s) 706
AjiI CACGTC 1 cut(s) 888
AluBI AGCT 5 cut(s) 350, 365, 665, 683, 726
AluI AGCT 5 cut(s) 350, 365, 665, 683, 726
Alw26I GTCTC 1 cut(s) 443
AlwI GGATC 6 cut(s) 158, 171, 386, 399, 818, 846
AlwNI CAGNNNCTG 1 cut(s) 245
AoxI GGCC 4 cut(s) 460, 488, 651, 740
ApeKI GCWGC 3 cut(s) 302, 365, 443
AsiGI ACCGGT 1 cut(s) 706
AspS9I GGNCC 3 cut(s) 4, 605, 652
AsuC2I CCSGG 3 cut(s) 573, 638, 656
AsuHPI GGTGA 2 cut(s) 318, 738
AvaII GGWCC 2 cut(s) 4, 605
BamHI GGATCC 2 cut(s) 163, 391
BanI GGYRCC 1 cut(s) 278
BbvCI CCTCAGC 1 cut(s) 132
BbvI GCAGC 3 cut(s) 314, 352, 430
BccI CCATC 2 cut(s) 43, 751
BceAI ACGGC 1 cut(s) 568
BcnI CCSGG 3 cut(s) 573, 638, 656
BcoDI GTCTC 1 cut(s) 443
BfaI CTAG 2 cut(s) 338, 458
BfmI CTRYAG 1 cut(s) 544
BisI GCNGC 4 cut(s) 282, 303, 366, 444
BlsI GCNGC 4 cut(s) 283, 304, 367, 445
Bme1390I CCNGG 3 cut(s) 573, 638, 656
Bme18I GGWCC 2 cut(s) 4, 605
BmgBI CACGTC 1 cut(s) 888
BmgT120I GGNCC 3 cut(s) 4, 605, 652
BmrFI CCNGG 3 cut(s) 573, 638, 656
BmsI GCATC 2 cut(s) 105, 289
BpmI CTGGAG 3 cut(s) 240, 645, 663
Bpu10I CCTNAGC 2 cut(s) 132, 684
BpuMI CCSGG 3 cut(s) 573, 638, 656
BsaI GGTCTC 1 cut(s) 443
BsaJI CCNNGG 4 cut(s) 23, 571, 636, 654
BsaWI WCCGGW 4 cut(s) 403, 472, 613, 706
Bsc4I CCNNNNNNNGG 4 cut(s) 275, 539, 637, 643
Bse118I RCCGGY 1 cut(s) 706
Bse1I ACTGG 1 cut(s) 692
Bse3DI GCAATG 1 cut(s) 868
BseDI CCNNGG 4 cut(s) 23, 571, 636, 654
BseGI GGATG 4 cut(s) 54, 331, 409, 469
BseLI CCNNNNNNNGG 4 cut(s) 275, 539, 637, 643
BseMI GCAATG 1 cut(s) 868
BseMII CTCAG 3 cut(s) 146, 438, 698
BseNI ACTGG 1 cut(s) 692
BseRI GAGGAG 3 cut(s) 243, 303, 615
BseXI GCAGC 3 cut(s) 314, 352, 430
BshFI GGCC 4 cut(s) 462, 490, 653, 742
BshNI GGYRCC 1 cut(s) 278
BshTI ACCGGT 1 cut(s) 706
BslI CCNNNNNNNGG 4 cut(s) 275, 539, 637, 643
BsmAI GTCTC 1 cut(s) 443
BsmI GAATGC 1 cut(s) 919
BsnI GGCC 4 cut(s) 462, 490, 653, 742
Bso31I GGTCTC 1 cut(s) 443
Bsp1407I TGTACA 1 cut(s) 67
Bsp143I GATC 5 cut(s) 112, 163, 391, 810, 838
BspACI CCGC 4 cut(s) 161, 282, 395, 633
BspANI GGCC 4 cut(s) 462, 490, 653, 742
BspCNI CTCAG 3 cut(s) 145, 439, 697
BspPI GGATC 6 cut(s) 158, 171, 386, 399, 818, 846
BspT107I GGYRCC 1 cut(s) 278
BspTNI GGTCTC 1 cut(s) 443
BsrDI GCAATG 1 cut(s) 868
BsrFI RCCGGY 1 cut(s) 706
BsrGI TGTACA 1 cut(s) 67
BsrI ACTGG 1 cut(s) 692
BssAI RCCGGY 1 cut(s) 706
BssECI CCNNGG 4 cut(s) 23, 571, 636, 654
BssMI GATC 5 cut(s) 112, 163, 391, 810, 838
Bst4CI ACNGT 3 cut(s) 104, 806, 846
BstAUI TGTACA 1 cut(s) 67
BstC8I GCNNGC 1 cut(s) 246
BstDEI CTNAG 3 cut(s) 132, 447, 684
BstF5I GGATG 4 cut(s) 54, 331, 409, 469
BstKTI GATC 5 cut(s) 115, 166, 394, 813, 841
BstMAI GTCTC 1 cut(s) 443
BstMBI GATC 5 cut(s) 112, 163, 391, 810, 838
BstMWI GCNNNNNNNGC 3 cut(s) 347, 374, 573
BstNSI RCATGY 1 cut(s) 44
BstSCI CCNGG 3 cut(s) 571, 636, 654
BstSFI CTRYAG 1 cut(s) 544
BstV1I GCAGC 3 cut(s) 314, 352, 430
BstX2I RGATCY 2 cut(s) 163, 391
BstYI RGATCY 2 cut(s) 163, 391
BsuRI GGCC 4 cut(s) 462, 490, 653, 742
BtrI CACGTC 1 cut(s) 888
BtsCI GGATG 4 cut(s) 54, 331, 409, 469
BtsIMutI CAGTG 1 cut(s) 756
Cac8I GCNNGC 1 cut(s) 246
CaiI CAGNNNCTG 1 cut(s) 245
Cfr10I RCCGGY 1 cut(s) 706
Cfr13I GGNCC 3 cut(s) 4, 605, 652
CseI GACGC 1 cut(s) 805
Csp6I GTAC 2 cut(s) 68, 478
CspAI ACCGGT 1 cut(s) 706
CspCI CAANNNNNGTGG 2 cut(s) 420, 455
CviAII CATG 2 cut(s) 41, 92
CviQI GTAC 2 cut(s) 68, 478
DdeI CTNAG 3 cut(s) 132, 447, 684
DpnI GATC 5 cut(s) 114, 165, 393, 812, 840
DpnII GATC 5 cut(s) 112, 163, 391, 810, 838
EciI GGCGGA 2 cut(s) 176, 384
Eco31I GGTCTC 1 cut(s) 443
Eco47I GGWCC 2 cut(s) 4, 605
Eco57I CTGAAG 1 cut(s) 381
EcoO109I RGGNCCY 1 cut(s) 605
FaeI CATG 2 cut(s) 44, 95
FatI CATG 2 cut(s) 40, 91
FauI CCCGC 1 cut(s) 640
FauNDI CATATG 1 cut(s) 215
Fnu4HI GCNGC 4 cut(s) 282, 303, 366, 444
FokI GGATG 4 cut(s) 61, 318, 396, 456
Fsp4HI GCNGC 4 cut(s) 282, 303, 366, 444
FspBI CTAG 2 cut(s) 338, 458
GluI GCNGC 4 cut(s) 282, 303, 366, 444
GsuI CTGGAG 3 cut(s) 240, 645, 663
HaeIII GGCC 4 cut(s) 462, 490, 653, 742
HgaI GACGC 1 cut(s) 805
Hin1II CATG 2 cut(s) 44, 95
HincII GTYRAC 1 cut(s) 144
HindII GTYRAC 1 cut(s) 144
HinfI GANTC 1 cut(s) 323
HpaI GTTAAC 1 cut(s) 144
HphI GGTGA 2 cut(s) 318, 738
Hpy166II GTNNAC 3 cut(s) 68, 144, 478
Hpy188I TCNGA 3 cut(s) 27, 48, 912
Hpy188III TCNNGA 3 cut(s) 167, 296, 359
Hpy8I GTNNAC 3 cut(s) 68, 144, 478
Hpy99I CGWCG 2 cut(s) 892, 905
HpyAV CCTTC 1 cut(s) 269
HpyCH4III ACNGT 3 cut(s) 104, 806, 846
HpyCH4IV ACGT 1 cut(s) 887
HpyCH4V TGCA 1 cut(s) 302
HpyF10VI GCNNNNNNNGC 3 cut(s) 347, 374, 573
HpyF3I CTNAG 3 cut(s) 132, 447, 684
HpySE526I ACGT 1 cut(s) 887
Hsp92II CATG 2 cut(s) 44, 95
KspAI GTTAAC 1 cut(s) 144
Kzo9I GATC 5 cut(s) 112, 163, 391, 810, 838
LmnI GCTCC 7 cut(s) 355, 646, 652, 664, 670, 676, 682
Lsp1109I GCAGC 3 cut(s) 314, 352, 430
LweI GCATC 2 cut(s) 105, 289
MaeI CTAG 2 cut(s) 338, 458
MaeII ACGT 1 cut(s) 887
MaeIII GTNAC 2 cut(s) 715, 754
MalI GATC 5 cut(s) 114, 165, 393, 812, 840
MboI GATC 5 cut(s) 112, 163, 391, 810, 838
MboII GAAGA 3 cut(s) 85, 184, 214
MflI RGATCY 2 cut(s) 163, 391
MluCI AATT 1 cut(s) 11
MmeI TCCRAC 1 cut(s) 341
MseI TTAA 3 cut(s) 78, 143, 189
MslI CAYNNNNRTG 1 cut(s) 831
MspR9I CCNGG 3 cut(s) 573, 638, 656
Mva1269I GAATGC 1 cut(s) 919
MwoI GCNNNNNNNGC 3 cut(s) 347, 374, 573
NciI CCSGG 3 cut(s) 573, 638, 656
NdeI CATATG 1 cut(s) 215
NdeII GATC 5 cut(s) 112, 163, 391, 810, 838
NlaIII CATG 2 cut(s) 44, 95
NmeAIII GCCGAG 1 cut(s) 403
NmuCI GTSAC 2 cut(s) 715, 754
NspI RCATGY 1 cut(s) 44
PciI ACATGT 1 cut(s) 40
PctI GAATGC 1 cut(s) 919
PfeI GAWTC 1 cut(s) 323
PinAI ACCGGT 1 cut(s) 706
PkrI GCNGC 4 cut(s) 283, 304, 367, 445
PpuMI RGGWCCY 1 cut(s) 605
PscI ACATGT 1 cut(s) 40
Psp5II RGGWCCY 1 cut(s) 605
PspPI GGNCC 3 cut(s) 4, 605, 652
PspPPI RGGWCCY 1 cut(s) 605
PstNI CAGNNNCTG 1 cut(s) 245
PsuI RGATCY 2 cut(s) 163, 391
RsaI GTAC 2 cut(s) 69, 479
RsaNI GTAC 2 cut(s) 68, 478
RseI CAYNNNNRTG 1 cut(s) 831
SaqAI TTAA 3 cut(s) 78, 143, 189
SatI GCNGC 4 cut(s) 282, 303, 366, 444
Sau3AI GATC 5 cut(s) 112, 163, 391, 810, 838
Sau96I GGNCC 3 cut(s) 4, 605, 652
ScrFI CCNGG 3 cut(s) 573, 638, 656
SfaNI GCATC 2 cut(s) 105, 289
SfcI CTRYAG 1 cut(s) 544
SinI GGWCC 2 cut(s) 4, 605
SmiMI CAYNNNNRTG 1 cut(s) 831
Sse9I AATT 1 cut(s) 11
SsiI CCGC 4 cut(s) 161, 282, 395, 633
SspMI CTAG 2 cut(s) 338, 458
StyD4I CCNGG 3 cut(s) 571, 636, 654
TaaI ACNGT 3 cut(s) 104, 806, 846
TaiI ACGT 1 cut(s) 890
TaqI TCGA 1 cut(s) 890
TasI AATT 1 cut(s) 11
TatI WGTACW 1 cut(s) 67
TauI GCSGC 1 cut(s) 284
TfiI GAWTC 1 cut(s) 323
Tru1I TTAA 3 cut(s) 78, 143, 189
Tru9I TTAA 3 cut(s) 78, 143, 189
TscAI CASTG 1 cut(s) 763
TseFI GTSAC 2 cut(s) 715, 754
TseI GCWGC 3 cut(s) 302, 365, 443
Tsp45I GTSAC 2 cut(s) 715, 754
TspDTI ATGAA 1 cut(s) 500
TspRI CASTG 1 cut(s) 763
VpaK11BI GGWCC 2 cut(s) 4, 605
XceI RCATGY 1 cut(s) 44
XspI CTAG 2 cut(s) 338, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.