MD13G1161800.v1.1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0015599
Physical Location & Seq
Forward (+)
12737763 .. 12739560
1798 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1161800.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGGAGAAACCTCGGGTGACGGAGATTCATGTTCGAATGGACTGTAACGGATGTGTGCAGAAGATAAAGAAAGCGCTACATGGCATCAATGGTATATATGAGCTTTACATTGACTTCCCACAACAAAAGTTGACAATAATTGGGTGGGCGGATCCGGAGAAGATAGTAAAAGCGATTAAGAAGACGAGGAAGATTGCCACCATATGTTCCCACACAGAACAACAAACAGAGCCTGCTCCTCAGCCGCCAGAACAAGCACCGGAGAATGGTGCACCAGCTCCTGAGGTGGCAAATCCTCCTCCGGCAGAAGCACCACCACCGGCTGAGGCAGCTCCGCCAGCTGAAGCTGCCTCGCCAGCAGAGCCACCGAGAGAATCACCACCGCCTCAACAGCCAGCACCGGAGCCAGCAACGCCAACACCTGTAGCTGCTGAGACTAACCCCGGGCAACAAATACACCACCAGAGACCAAGAGACGTTGGAGAAGTTCATACAATTTACCACCAGAGTCACCACCCACCGGGACAATCACCACCGCCTCAACAGCCAGCACCAGAGTCAGCAGCGCCAACACCTGTAGCTGCTGAGACTAACCCTGGGCAACAAATACACCACCAGAGACGAAGAGACGTTGGAGAAGTTCATACCATATACCACCACGGTAACCCTCCACCTGATTATGGCTATAGATATGGCTACTCTCAAGGCTACAATGGCTACTGGAACGGATACCATAACAGCCAAGGACCTCCACCGGAGCCTGCCCCTATCCCCACCCCAATGGGCCCTTCCCCGATGACCCCCGCCACAATGGGCCCTCCTCCGATGAGCCCCACCACAATGAGTCACACCCCACCTCCACCTATGTATGTAACACACAGCTACAACACATACAGGCCATCACCTTTTGTTACAGAATATCGATACATCCAGCCGCCTCCACAACCCACACATTTGAGCAGGACGAACCATTACAACGAGCATTTCAGGAGGACGAACCATTACAACGAGGAGCAGCACTATGTCAACGGGAGCGGCAACGGAAACATCACATCAATTTTTAGCGACGAAAATCCAAATGCGTGCACCGTAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

366

Amino Acids

40.22

Weight (kDa)

6.25

Isoelectric Point (pI)

67.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 11 - 62 3e-12 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015599)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g18950
malus_domestica MD13G1161800.v1.1 MD16G1161600.v1.1
prunus_persica Prupe.1G129100_v2.0.a1
pyrus_communis pycom16g13700
rosa_chinensis RchiOBHm_Chr4g0423841
rosa_laevigata RLG00000007527
rosa_multiflora Rmu_sc0003369.1_g000009 Rmu_sc0008198.1_g000001
rosa_roxburghii Rroxscaffold_5G00365960
rosa_rugosa Rorug04G0191200 Rorug04G0191200
rosa_samantha Rh4AG250600 Rh4BG255900 Rh4DG250400
rosa_wichuraiana Rw4G021470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1035
AccIII TCCGGA 1 cut(s) 154
AciI CCGC 8 cut(s) 149, 245, 335, 383, 536, 804, 935, 1035
AclWI GGATC 2 cut(s) 146, 159
AcuI CTGAAG 1 cut(s) 363
AfeI AGCGCT 1 cut(s) 75
AfiI CCNNNNNNNGG 3 cut(s) 266, 520, 680
AjnI CCWGG 1 cut(s) 595
AjuI GAANNNNNNNTTGG 2 cut(s) 772, 804
AluBI AGCT 8 cut(s) 103, 278, 332, 341, 347, 428, 581, 882
AluI AGCT 8 cut(s) 103, 278, 332, 341, 347, 428, 581, 882
Alw21I GWGCWC 2 cut(s) 274, 1088
Alw26I GTCTC 6 cut(s) 428, 460, 468, 581, 613, 621
Alw44I GTGCAC 2 cut(s) 270, 1084
AlwI GGATC 2 cut(s) 146, 159
AlwNI CAGNNNCTG 2 cut(s) 233, 281
Ama87I CYCGRG 2 cut(s) 12, 443
Aor13HI TCCGGA 1 cut(s) 154
Aor51HI AGCGCT 1 cut(s) 75
AoxI GGCC 3 cut(s) 784, 814, 896
ApaI GGGCCC 2 cut(s) 788, 818
ApaLI GTGCAC 2 cut(s) 270, 1084
ApeKI GCWGC 6 cut(s) 329, 347, 428, 563, 581, 1015
AspLEI GCGC 2 cut(s) 76, 568
AspS9I GGNCC 5 cut(s) 746, 784, 785, 814, 815
AsuC2I CCSGG 3 cut(s) 444, 445, 522
AsuHPI GGTGA 5 cut(s) 28, 369, 503, 522, 894
AsuII TTCGAA 1 cut(s) 34
AvaI CYCGRG 2 cut(s) 12, 443
AvaII GGWCC 1 cut(s) 746
AxyI CCTNAGG 1 cut(s) 282
BaeGI GKGCMC 4 cut(s) 274, 788, 818, 1088
BamHI GGATCC 1 cut(s) 151
BanII GRGCYC 3 cut(s) 788, 818, 833
BbsI GAAGAC 1 cut(s) 188
Bbv12I GWGCWC 2 cut(s) 274, 1088
BbvCI CCTCAGC 2 cut(s) 240, 324
BbvI GCAGC 6 cut(s) 334, 341, 415, 568, 575, 1027
BccI CCATC 1 cut(s) 907
BciT130I CCWGG 1 cut(s) 597
BciVI GTATCC 1 cut(s) 722
BcnI CCSGG 3 cut(s) 444, 445, 522
BcoDI GTCTC 6 cut(s) 428, 460, 468, 581, 613, 621
BfmI CTRYAG 3 cut(s) 423, 576, 685
BfoI RGCGCY 2 cut(s) 77, 569
BfuI GTATCC 1 cut(s) 722
BisI GCNGC 9 cut(s) 245, 330, 348, 429, 564, 582, 935, 1016, 1036
BlsI GCNGC 9 cut(s) 246, 331, 349, 430, 565, 583, 936, 1017, 1037
Bme1390I CCNGG 4 cut(s) 444, 445, 522, 597
Bme18I GGWCC 1 cut(s) 746
BmeT110I CYCGRG 2 cut(s) 12, 443
BmgT120I GGNCC 5 cut(s) 746, 784, 785, 814, 815
BmiI GGNNCC 5 cut(s) 153, 405, 759, 786, 816
BmrFI CCNGG 4 cut(s) 444, 445, 522, 597
BmsI GCATC 1 cut(s) 93
BpiI GAAGAC 1 cut(s) 188
Bpu10I CCTNAGC 2 cut(s) 240, 324
Bpu14I TTCGAA 1 cut(s) 34
BpuEI CTTGAG 1 cut(s) 687
BpuMI CCSGG 3 cut(s) 444, 445, 522
Bsa29I ATCGAT 1 cut(s) 922
BsaI GGTCTC 1 cut(s) 460
BsaJI CCNNGG 7 cut(s) 11, 442, 443, 595, 596, 658, 742
BsaWI WCCGGW 4 cut(s) 154, 259, 400, 754
BsaXI ACNNNNNCTCC 2 cut(s) 841, 871
Bsc4I CCNNNNNNNGG 3 cut(s) 266, 520, 680
Bse118I RCCGGY 1 cut(s) 319
Bse1I ACTGG 1 cut(s) 725
Bse21I CCTNAGG 1 cut(s) 282
BseAI TCCGGA 1 cut(s) 154
BseBI CCWGG 1 cut(s) 597
BseCI ATCGAT 1 cut(s) 922
BseDI CCNNGG 7 cut(s) 11, 442, 443, 595, 596, 658, 742
BseGI GGATG 2 cut(s) 56, 927
BseLI CCNNNNNNNGG 3 cut(s) 266, 520, 680
BseMII CTCAG 5 cut(s) 254, 273, 315, 423, 576
BseNI ACTGG 1 cut(s) 725
BseRI GAGGAG 4 cut(s) 228, 288, 810, 1025
BseSI GKGCMC 4 cut(s) 274, 788, 818, 1088
BseXI GCAGC 6 cut(s) 334, 341, 415, 568, 575, 1027
BsgI GTGCAG 1 cut(s) 77
BshFI GGCC 3 cut(s) 786, 816, 898
BshVI ATCGAT 1 cut(s) 922
BsiHKAI GWGCWC 2 cut(s) 274, 1088
BsiHKCI CYCGRG 2 cut(s) 12, 443
BsiSI CCGG 8 cut(s) 155, 260, 302, 320, 401, 444, 521, 755
BslFI GGGAC 1 cut(s) 537
BslI CCNNNNNNNGG 3 cut(s) 266, 520, 680
BsmAI GTCTC 6 cut(s) 428, 460, 468, 581, 613, 621
BsmBI CGTCTC 3 cut(s) 468, 613, 621
BsmFI GGGAC 1 cut(s) 537
BsnI GGCC 3 cut(s) 786, 816, 898
Bso31I GGTCTC 1 cut(s) 460
BsoBI CYCGRG 2 cut(s) 12, 443
Bsp119I TTCGAA 1 cut(s) 34
Bsp120I GGGCCC 2 cut(s) 784, 814
Bsp1286I GDGCHC 5 cut(s) 274, 788, 818, 833, 1088
Bsp13I TCCGGA 1 cut(s) 154
Bsp143I GATC 1 cut(s) 151
BspACI CCGC 8 cut(s) 149, 245, 335, 383, 536, 804, 935, 1035
BspANI GGCC 3 cut(s) 786, 816, 898
BspCNI CTCAG 5 cut(s) 253, 274, 316, 424, 577
BspDI ATCGAT 1 cut(s) 922
BspEI TCCGGA 1 cut(s) 154
BspLI GGNNCC 5 cut(s) 153, 405, 759, 786, 816
BspPI GGATC 2 cut(s) 146, 159
BspT104I TTCGAA 1 cut(s) 34
BspTNI GGTCTC 1 cut(s) 460
BsrBI CCGCTC 1 cut(s) 1035
BsrFI RCCGGY 1 cut(s) 319
BsrI ACTGG 1 cut(s) 725
BssAI RCCGGY 1 cut(s) 319
BssECI CCNNGG 7 cut(s) 11, 442, 443, 595, 596, 658, 742
BssMI GATC 1 cut(s) 151
BssT1I CCWWGG 1 cut(s) 742
Bst2UI CCWGG 1 cut(s) 597
Bst4CI ACNGT 3 cut(s) 44, 662, 1090
Bst6I CTCTTC 1 cut(s) 619
BstBI TTCGAA 1 cut(s) 34
BstC8I GCNNGC 8 cut(s) 234, 339, 357, 396, 408, 549, 762, 1084
BstDEI CTNAG 5 cut(s) 240, 282, 324, 432, 585
BstDSI CCRYGG 1 cut(s) 658
BstEII GGTNACC 1 cut(s) 662
BstF5I GGATG 2 cut(s) 56, 927
BstH2I RGCGCY 2 cut(s) 77, 569
BstHHI GCGC 2 cut(s) 76, 568
BstKTI GATC 1 cut(s) 154
BstMAI GTCTC 6 cut(s) 428, 460, 468, 581, 613, 621
BstMBI GATC 1 cut(s) 151
BstMWI GCNNNNNNNGC 9 cut(s) 329, 338, 347, 356, 361, 391, 412, 544, 714
BstNI CCWGG 1 cut(s) 597
BstPI GGTNACC 1 cut(s) 662
BstSCI CCNGG 4 cut(s) 442, 443, 520, 595
BstSFI CTRYAG 3 cut(s) 423, 576, 685
BstSLI GKGCMC 4 cut(s) 274, 788, 818, 1088
BstV1I GCAGC 6 cut(s) 334, 341, 415, 568, 575, 1027
BstV2I GAAGAC 1 cut(s) 188
BstX2I RGATCY 1 cut(s) 151
BstXI CCANNNNNNTGG 1 cut(s) 781
BstYI RGATCY 1 cut(s) 151
Bsu15I ATCGAT 1 cut(s) 922
Bsu36I CCTNAGG 1 cut(s) 282
BsuI GTATCC 1 cut(s) 722
BsuRI GGCC 3 cut(s) 786, 816, 898
BsuTUI ATCGAT 1 cut(s) 922
BtgI CCRYGG 1 cut(s) 658
BtsCI GGATG 2 cut(s) 56, 927
Cac8I GCNNGC 8 cut(s) 234, 339, 357, 396, 408, 549, 762, 1084
CaiI CAGNNNCTG 2 cut(s) 233, 281
CfoI GCGC 2 cut(s) 76, 568
Cfr10I RCCGGY 1 cut(s) 319
Cfr13I GGNCC 5 cut(s) 746, 784, 785, 814, 815
Cfr9I CCCGGG 1 cut(s) 443
ClaI ATCGAT 1 cut(s) 922
CviAII CATG 2 cut(s) 29, 80
DdeI CTNAG 5 cut(s) 240, 282, 324, 432, 585
DpnI GATC 1 cut(s) 153
DpnII GATC 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 619
EarI CTCTTC 1 cut(s) 619
EciI GGCGGA 2 cut(s) 164, 324
Eco130I CCWWGG 1 cut(s) 742
Eco24I GRGCYC 3 cut(s) 788, 818, 833
Eco31I GGTCTC 1 cut(s) 460
Eco47I GGWCC 1 cut(s) 746
Eco47III AGCGCT 1 cut(s) 75
Eco57I CTGAAG 1 cut(s) 363
Eco81I CCTNAGG 1 cut(s) 282
Eco88I CYCGRG 2 cut(s) 12, 443
Eco91I GGTNACC 1 cut(s) 662
EcoO109I RGGNCCY 3 cut(s) 746, 785, 815
EcoO65I GGTNACC 1 cut(s) 662
EcoRII CCWGG 1 cut(s) 595
EcoT14I CCWWGG 1 cut(s) 742
EcoT38I GRGCYC 3 cut(s) 788, 818, 833
ErhI CCWWGG 1 cut(s) 742
Esp3I CGTCTC 3 cut(s) 468, 613, 621
FaeI CATG 2 cut(s) 32, 83
FaqI GGGAC 1 cut(s) 537
FatI CATG 2 cut(s) 28, 79
FauI CCCGC 1 cut(s) 811
FauNDI CATATG 1 cut(s) 203
Fnu4HI GCNGC 9 cut(s) 245, 330, 348, 429, 564, 582, 935, 1016, 1036
FokI GGATG 2 cut(s) 63, 914
FriOI GRGCYC 3 cut(s) 788, 818, 833
Fsp4HI GCNGC 9 cut(s) 245, 330, 348, 429, 564, 582, 935, 1016, 1036
GlaI GCGC 2 cut(s) 75, 567
GluI GCNGC 9 cut(s) 245, 330, 348, 429, 564, 582, 935, 1016, 1036
HaeII RGCGCY 2 cut(s) 77, 569
HaeIII GGCC 3 cut(s) 786, 816, 898
HapII CCGG 8 cut(s) 155, 260, 302, 320, 401, 444, 521, 755
HhaI GCGC 2 cut(s) 76, 568
Hin1II CATG 2 cut(s) 32, 83
Hin6I GCGC 2 cut(s) 74, 566
HinP1I GCGC 2 cut(s) 74, 566
HincII GTYRAC 2 cut(s) 132, 1027
HindII GTYRAC 2 cut(s) 132, 1027
HinfI GANTC 5 cut(s) 25, 374, 508, 557, 844
HpaII CCGG 8 cut(s) 155, 260, 302, 320, 401, 444, 521, 755
HphI GGTGA 5 cut(s) 28, 369, 503, 522, 894
Hpy166II GTNNAC 4 cut(s) 132, 272, 1027, 1086
Hpy188I TCNGA 1 cut(s) 825
Hpy188III TCNNGA 3 cut(s) 155, 281, 988
Hpy8I GTNNAC 4 cut(s) 132, 272, 1027, 1086
Hpy99I CGWCG 1 cut(s) 1070
HpyAV CCTTC 1 cut(s) 798
HpyCH4III ACNGT 3 cut(s) 44, 662, 1090
HpyCH4IV ACGT 2 cut(s) 477, 630
HpyCH4V TGCA 3 cut(s) 58, 272, 1086
HpyF10VI GCNNNNNNNGC 9 cut(s) 329, 338, 347, 356, 361, 391, 412, 544, 714
HpyF3I CTNAG 5 cut(s) 240, 282, 324, 432, 585
HpySE526I ACGT 2 cut(s) 477, 630
Hsp92II CATG 2 cut(s) 32, 83
HspAI GCGC 2 cut(s) 74, 566
Kpn2I TCCGGA 1 cut(s) 154
Kzo9I GATC 1 cut(s) 151
LmnI GCTCC 7 cut(s) 241, 283, 337, 403, 757, 1012, 1032
Lsp1109I GCAGC 6 cut(s) 334, 341, 415, 568, 575, 1027
LweI GCATC 1 cut(s) 93
MaeII ACGT 2 cut(s) 477, 630
MaeIII GTNAC 7 cut(s) 16, 44, 509, 662, 845, 871, 910
MalI GATC 1 cut(s) 153
MbiI CCGCTC 1 cut(s) 1035
MboI GATC 1 cut(s) 151
MboII GAAGA 5 cut(s) 73, 172, 193, 202, 636
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 5 cut(s) 274, 788, 818, 833, 1088
MluCI AATT 3 cut(s) 138, 495, 1056
MlyI GAGTC 3 cut(s) 517, 566, 853
MmeI TCCRAC 2 cut(s) 460, 613
MroI TCCGGA 1 cut(s) 154
MseI TTAA 1 cut(s) 177
MslI CAYNNNNRTG 3 cut(s) 779, 839, 1091
MspA1I CMGCKG 1 cut(s) 341
MspI CCGG 8 cut(s) 155, 260, 302, 320, 401, 444, 521, 755
MspR9I CCNGG 4 cut(s) 444, 445, 522, 597
MvaI CCWGG 1 cut(s) 597
MwoI GCNNNNNNNGC 9 cut(s) 329, 338, 347, 356, 361, 391, 412, 544, 714
NciI CCSGG 3 cut(s) 444, 445, 522
NdeI CATATG 1 cut(s) 203
NdeII GATC 1 cut(s) 151
NlaIII CATG 2 cut(s) 32, 83
NlaIV GGNNCC 5 cut(s) 153, 405, 759, 786, 816
NmuCI GTSAC 3 cut(s) 16, 509, 845
NspV TTCGAA 1 cut(s) 34
PasI CCCWGGG 1 cut(s) 596
PfeI GAWTC 2 cut(s) 25, 374
PkrI GCNGC 9 cut(s) 246, 331, 349, 430, 565, 583, 936, 1017, 1037
PleI GAGTC 3 cut(s) 516, 565, 852
PpsI GAGTC 3 cut(s) 516, 565, 852
PpuMI RGGWCCY 1 cut(s) 746
Psp5II RGGWCCY 1 cut(s) 746
Psp6I CCWGG 1 cut(s) 595
PspEI GGTNACC 1 cut(s) 662
PspGI CCWGG 1 cut(s) 595
PspN4I GGNNCC 5 cut(s) 153, 405, 759, 786, 816
PspOMI GGGCCC 2 cut(s) 784, 814
PspPI GGNCC 5 cut(s) 746, 784, 785, 814, 815
PspPPI RGGWCCY 1 cut(s) 746
PstNI CAGNNNCTG 2 cut(s) 233, 281
PsuI RGATCY 1 cut(s) 151
PvuII CAGCTG 1 cut(s) 341
RseI CAYNNNNRTG 3 cut(s) 779, 839, 1091
SaqAI TTAA 1 cut(s) 177
SatI GCNGC 9 cut(s) 245, 330, 348, 429, 564, 582, 935, 1016, 1036
Sau3AI GATC 1 cut(s) 151
Sau96I GGNCC 5 cut(s) 746, 784, 785, 814, 815
SchI GAGTC 3 cut(s) 517, 566, 853
ScrFI CCNGG 4 cut(s) 444, 445, 522, 597
SduI GDGCHC 5 cut(s) 274, 788, 818, 833, 1088
SfaNI GCATC 1 cut(s) 93
SfcI CTRYAG 3 cut(s) 423, 576, 685
SfuI TTCGAA 1 cut(s) 34
SinI GGWCC 1 cut(s) 746
SmaI CCCGGG 1 cut(s) 445
SmiMI CAYNNNNRTG 3 cut(s) 779, 839, 1091
SmlI CTYRAG 1 cut(s) 702
SmoI CTYRAG 1 cut(s) 702
Sse9I AATT 3 cut(s) 138, 495, 1056
SsiI CCGC 8 cut(s) 149, 245, 335, 383, 536, 804, 935, 1035
StyD4I CCNGG 4 cut(s) 442, 443, 520, 595
StyI CCWWGG 1 cut(s) 742
TaaI ACNGT 3 cut(s) 44, 662, 1090
TaiI ACGT 2 cut(s) 480, 633
TaqI TCGA 2 cut(s) 34, 922
TasI AATT 3 cut(s) 138, 495, 1056
TauI GCSGC 3 cut(s) 247, 937, 1038
TfiI GAWTC 2 cut(s) 25, 374
Tru1I TTAA 1 cut(s) 177
Tru9I TTAA 1 cut(s) 177
TseFI GTSAC 3 cut(s) 16, 509, 845
TseI GCWGC 6 cut(s) 329, 347, 428, 563, 581, 1015
Tsp45I GTSAC 3 cut(s) 16, 509, 845
TspDTI ATGAA 3 cut(s) 17, 479, 632
TspGWI ACGGA 4 cut(s) 35, 63, 741, 1056
TspMI CCCGGG 1 cut(s) 443
VneI GTGCAC 2 cut(s) 270, 1084
VpaK11BI GGWCC 1 cut(s) 746
XmaI CCCGGG 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.