FvH4_4g19350

beta-D-xylosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012799
Physical Location & Seq
Reverse (-)
23020168 .. 23025072
4905 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g19350.t3

Sequence Viewer

Length: 2319 bp
ATGGCTTCTGGTTACAATAACAAGCTTTCCCTTCTTGCTCTTGTTCTCTGTGTATCAGCATTGTTGTTTAATCTGGTACATGCGCGTCCACCCTTTGCGTGCGACCCCCGGAACCCGTTAACGAGAGGGTTCAAGTTCTGCAGGACTAGGGTGCCGGTCCACGTCAGGGTTCAGGACCTGATCGGACGGCTGACACTGCAGGAGAAGATTAGGCTGCTGGTGAACAATGCCATAGCTGTGCCGAGGCTTGGCATTCAGGGCTACGAGTGGTGGTCGGAGGCGCTTCACGGCGTCTCCAATGTCGGCCCCGGAACTAAGTTTGGCGGGGCTTTTCCGGGAGCCACTAGCTTCCCCCAAGTCATCACCACCGCCGCTTCCTTCAATCAATCACTTTGGCAAGAAATCGGACAGGTGGTGTCTGATGAAGCAAGAGCAATGTACAATGGAGGCCAAGCTGGTTTGACATATTGGAGCCCTAACGTGAACATATTCCGTGACCCGCGATGGGGAAGAGGACAAGAGACTCCTGGGGAAGACCCTGTCTTGGCTGCTAAGTATGCTGCTAGCTATGTCAAGGGTCTCCAAGGCGACGGAGCCGGAAATCGGCTTAAGGTTGCTGCCTGTTGCAAACATTACACTGCCTATGATCTCGACAATTGGAATGGTGTCGATCGCTTCCATTTTAACGCCCGGGTTAGCAAGCAAGACTTGGCAGACACATATGACGTGCCATTTAGAGGATGTGTGTTAGAAGGAAAGGTTGCTAGTGTCATGTGTTCATACAACCAAGTTAATGGAAAGCCTACTTGTGCTGACCCTGATCTCCTAAAGAACACGATCCGTGGGGAATGGAAGCTCAATGGGTACATAGTCTCCGACTGTGACTCGGTGGGAGTGTTTTACGACCAACAACATTACACCAGGACACCGGAGGAGGCGGCGGCGGAGGCGATTAAAGCGGGTTTGGACCTGGATTGTGGGCCGTTCCTTGCGATCCATACGGAGGGTGCTATAAAGGCCGGCCTGCTACCAGAGATCAATGTGGACTATGCGCTAGCAAACACTCTTACAGTCCAAATGAGACTCGGGATGTTCGATGGCGAACCATCAGCCCAACAATATGGAAACTTGGGCCCAAGGGATGTTTGCACACTGGCCCACCAAGAGTTGGCACTAGAGGCTGCTAGGCAAGGCATTGTTTTGCTTCAAAACAATGGGCACACGCTGCCTCTATCTACTGTGCGTCACCATACAGTTGCCGTAATTGGGCCCAACTCGGATGTTACCGAAACGATGATAGGAAACTATGCCGGTGTTGCATGTGGCTACACAACTCCCCTGCAAGGAATTGGGAGGTACACAAAGACCATTCATCAACAAGGGTGCACTAATGTTGCTTGCACCACTAACCAACTATTTGGGGCAGCTGAGGCTGCAGCCAGGCAGGCCGAAGCGACCGTCCTAGTGATGGGCCTCGACCAATCTATTGAAGCAGAATTTCGGGATAGGACCGACCTGGTCATGCCAGGACACCAACAAGAGCTTGTGTCGAGAGTGGCTCAGGCCTCTAGAGGCCCAACCATACTGGTCTTGATGTCCGGTGGCCCAATTGACATGTCTTTTGCTAAGAACGACCCAAAAATTGGAGCCATAATTTGGGTTGGATATCCTGGTCAAGCAGGAGGAACTGCAATGGCTGATGTGCTGTTTGGTACTACCAATCCAAGTGGGAAGCTACCAATGACATGGTACCCTCAAGACTATGTATCCAAAGTGCCAATGACAAACATGGCCATGCGTGCCGGCAGAGGCTACCCCGGTAGGACGTACCGGTTTTACAAAGGTCCGGTGGTATTTCCCTTCGGTCTCGGCTTAAGCTACACTACCTTCCGCCACTCACTAGCCCAAGTTCCCACATCGGTGTCGGTACCCCTCACAAGTCTAAGTGCCACCACAAACTCAACCATGCTAAGCAGTGCTGTGAGAGTCAGCCACACAAATTGCAATCCACTCTCACTTGCTCTTCATGTTGATGTTAAAAACACCGGAGCTAGGGATGGAACTCACACTCTTCTAGTGTTCTCAAGTCCGCCTTCGGGGAAATGGGCCGTAAACAAGCAATTGGTGGGCTTTCACAAGGTCCATATTGTCGCCGGGTCTCAAAAACGGGTCAAGTTTGATGTCCATGTCTGCAAGCACCTCAGCGTTGTAGACCAGTTTGGGATTCAAAGAATTCCGATTGGTGAACATAAGCTTCAGATTGGAGATCTGGAGCATCACATCTCCGTTGAAGCCAATGTAGGAGAAATTAGATCATAA

Protein Analysis

773

Amino Acids

83.64

Weight (kDa)

8.46

Isoelectric Point (pI)

31.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_3 PF00933 111 - 352 7.6e-35 Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C PF01915 399 - 578 2.8e-42 Glycosyl hydrolase family 3 C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012799)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49360
fragaria_vesca FvH4_4g19350
malus_domestica MD13G1157400.v1.1 MD16G1158300.v1.1
prunus_persica Prupe.1G123100_v2.0.a1
pyrus_communis pycom16g13320
rosa_chinensis RchiOBHm_Chr4g0424381
rosa_laevigata RLG00000007479
rosa_multiflora Rmu_co8330357.1_g000001 Rmu_sc0003567.1_g000021 Rmu_sc0005995.1_g000003
rosa_roxburghii Rroxscaffold_5G00366330
rosa_rugosa Rorug04G0197100 Rorug04G0197200
rosa_samantha Rh4AG253200 Rh4CG269900 Rh4DG253000
rosa_wichuraiana Rw4G021850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 1749, 1927
AccB1I GGYRCC 3 cut(s) 151, 1749, 1927
AccB7I CCANNNNNTGG 3 cut(s) 1168, 1643, 1656
AccI GTMKAC 1 cut(s) 2211
AccII CGCG 2 cut(s) 85, 502
AclWI GGATC 2 cut(s) 832, 988
AcoI YGGCCR 1 cut(s) 1791
AcsI RAATTY 2 cut(s) 1496, 2232
AcuI CTGAAG 1 cut(s) 2240
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 8 cut(s) 78, 440, 866, 1358, 1714, 1751, 1829, 1929
AflII CTTAAG 2 cut(s) 608, 1873
AflIII ACRYGT 1 cut(s) 1614
AgeI ACCGGT 1 cut(s) 1830
AgsI TTSAA 6 cut(s) 133, 382, 1208, 1490, 2228, 2291
AjiI CACGTC 2 cut(s) 163, 727
AjnI CCWGG 7 cut(s) 526, 920, 969, 1439, 1515, 1525, 1669
AleI CACNNNNGTG 1 cut(s) 1919
Alw21I GWGCWC 1 cut(s) 1388
Alw26I GTCTC 7 cut(s) 298, 515, 584, 877, 1075, 1871, 2163
Alw44I GTGCAC 1 cut(s) 1384
AlwI GGATC 2 cut(s) 832, 988
AlwNI CAGNNNCTG 1 cut(s) 178
Ama87I CYCGRG 2 cut(s) 690, 1085
ApaI GGGCCC 2 cut(s) 1136, 1272
ApaLI GTGCAC 1 cut(s) 1384
ApeKI GCWGC 9 cut(s) 214, 548, 560, 617, 1181, 1225, 1424, 1433, 1436
ApoI RAATTY 2 cut(s) 1496, 2232
ArsI GACNNNNNNTTYG 4 cut(s) 1443, 1475, 1604, 1636
AsiGI ACCGGT 1 cut(s) 1830
Asp700I GAANNNNTTC 1 cut(s) 488
Asp718I GGTACC 2 cut(s) 1749, 1927
AspLEI GCGC 3 cut(s) 85, 283, 1054
AsuC2I CCSGG 7 cut(s) 109, 309, 336, 691, 692, 1818, 2155
AsuHPI GGTGA 4 cut(s) 232, 355, 1238, 2255
AsuNHI GCTAGC 2 cut(s) 563, 1054
AvaI CYCGRG 2 cut(s) 690, 1085
AvaII GGWCC 6 cut(s) 157, 175, 967, 1509, 1844, 2140
BaeGI GKGCMC 4 cut(s) 1136, 1221, 1272, 1388
BalI TGGCCA 1 cut(s) 1793
BanI GGYRCC 3 cut(s) 151, 1749, 1927
BanII GRGCYC 3 cut(s) 476, 1136, 1272
BbsI GAAGAC 1 cut(s) 540
Bbv12I GWGCWC 1 cut(s) 1388
BbvCI CCTCAGC 2 cut(s) 1428, 2201
BbvI GCAGC 9 cut(s) 201, 535, 547, 604, 1168, 1212, 1420, 1436, 1448
BccI CCATC 5 cut(s) 498, 1091, 1114, 1462, 2051
BceAI ACGGC 5 cut(s) 203, 304, 967, 1244, 2093
BciT130I CCWGG 7 cut(s) 528, 922, 971, 1441, 1517, 1527, 1671
BciVI GTATCC 1 cut(s) 1777
BcnI CCSGG 7 cut(s) 109, 309, 336, 691, 692, 1818, 2155
BcoDI GTCTC 7 cut(s) 298, 515, 584, 877, 1075, 1871, 2163
BfmI CTRYAG 3 cut(s) 139, 197, 1434
BfoI RGCGCY 1 cut(s) 284
BfrI CTTAAG 2 cut(s) 608, 1873
BfuI GTATCC 1 cut(s) 1777
BglI GCCNNNNNGGC 1 cut(s) 1445
BglII AGATCT 1 cut(s) 2266
BlpI GCTNAGC 1 cut(s) 1970
Bme18I GGWCC 6 cut(s) 157, 175, 967, 1509, 1844, 2140
BmeT110I CYCGRG 2 cut(s) 690, 1085
BmgBI CACGTC 2 cut(s) 163, 727
BmsI GCATC 1 cut(s) 2284
BmtI GCTAGC 2 cut(s) 567, 1058
BpiI GAAGAC 1 cut(s) 540
BplI GAGNNNNNCTC 2 cut(s) 1543, 1575
BpmI CTGGAG 1 cut(s) 2291
Bpu10I CCTNAGC 3 cut(s) 1428, 1560, 2201
Bpu1102I GCTNAGC 1 cut(s) 1970
BpuEI CTTGAG 2 cut(s) 1740, 2068
BpuMI CCSGG 7 cut(s) 109, 309, 336, 691, 692, 1818, 2155
BsaHI GRCGYC 1 cut(s) 291
BsaI GGTCTC 3 cut(s) 584, 1871, 2163
BsaJI CCNNGG 9 cut(s) 107, 242, 307, 527, 583, 690, 841, 1136, 1816
BsaWI WCCGGW 5 cut(s) 928, 1598, 1830, 1846, 2045
BsaXI ACNNNNNCTCC 4 cut(s) 278, 308, 857, 887
Bse118I RCCGGY 5 cut(s) 154, 1019, 1310, 1802, 1830
Bse1I ACTGG 3 cut(s) 1158, 1590, 2215
Bse3DI GCAATG 2 cut(s) 441, 1698
BseBI CCWGG 7 cut(s) 528, 922, 971, 1441, 1517, 1527, 1671
BseDI CCNNGG 9 cut(s) 107, 242, 307, 527, 583, 690, 841, 1136, 1816
BseGI GGATG 5 cut(s) 746, 1095, 1147, 1285, 2062
BseMI GCAATG 2 cut(s) 441, 1698
BseMII CTCAG 3 cut(s) 1419, 1574, 2215
BseNI ACTGG 3 cut(s) 1158, 1590, 2215
BseRI GAGGAG 1 cut(s) 947
BseSI GKGCMC 4 cut(s) 1136, 1221, 1272, 1388
BseXI GCAGC 9 cut(s) 201, 535, 547, 604, 1168, 1212, 1420, 1436, 1448
Bsh1236I CGCG 2 cut(s) 85, 502
Bsh1285I CGRYCG 2 cut(s) 673, 1458
BshNI GGYRCC 3 cut(s) 151, 1749, 1927
BshTI ACCGGT 1 cut(s) 1830
BsiEI CGRYCG 2 cut(s) 673, 1458
BsiHKAI GWGCWC 1 cut(s) 1388
BsiHKCI CYCGRG 2 cut(s) 690, 1085
BsmAI GTCTC 7 cut(s) 298, 515, 584, 877, 1075, 1871, 2163
BsmBI CGTCTC 1 cut(s) 298
BsmI GAATGC 1 cut(s) 252
Bso31I GGTCTC 3 cut(s) 584, 1871, 2163
BsoBI CYCGRG 2 cut(s) 690, 1085
Bsp120I GGGCCC 2 cut(s) 1132, 1268
Bsp1286I GDGCHC 5 cut(s) 476, 1136, 1221, 1272, 1388
Bsp1407I TGTACA 1 cut(s) 438
Bsp143I GATC 9 cut(s) 180, 646, 670, 820, 837, 993, 1035, 2266, 2312
Bsp1720I GCTNAGC 1 cut(s) 1970
BspCNI CTCAG 3 cut(s) 1420, 1573, 2214
BspFNI CGCG 2 cut(s) 85, 502
BspMAI CTGCAG 3 cut(s) 143, 201, 1438
BspOI GCTAGC 2 cut(s) 567, 1058
BspPI GGATC 2 cut(s) 832, 988
BspQI GCTCTTC 1 cut(s) 2028
BspT107I GGYRCC 3 cut(s) 151, 1749, 1927
BspTI CTTAAG 2 cut(s) 608, 1873
BspTNI GGTCTC 3 cut(s) 584, 1871, 2163
BsrDI GCAATG 2 cut(s) 441, 1698
BsrFI RCCGGY 5 cut(s) 154, 1019, 1310, 1802, 1830
BsrGI TGTACA 1 cut(s) 438
BsrI ACTGG 3 cut(s) 1158, 1590, 2215
BssAI RCCGGY 5 cut(s) 154, 1019, 1310, 1802, 1830
BssECI CCNNGG 9 cut(s) 107, 242, 307, 527, 583, 690, 841, 1136, 1816
BssMI GATC 9 cut(s) 180, 646, 670, 820, 837, 993, 1035, 2266, 2312
BssNI GRCGYC 1 cut(s) 291
BssT1I CCWWGG 2 cut(s) 583, 1136
Bst2UI CCWGG 7 cut(s) 528, 922, 971, 1441, 1517, 1527, 1671
Bst4CI ACNGT 5 cut(s) 881, 1072, 1240, 1255, 1459
Bst6I CTCTTC 3 cut(s) 505, 2028, 2076
BstACI GRCGYC 1 cut(s) 291
BstAFI CTTAAG 2 cut(s) 608, 1873
BstAPI GCANNNNNTGC 1 cut(s) 1225
BstAUI TGTACA 1 cut(s) 438
BstDEI CTNAG 8 cut(s) 315, 552, 1428, 1560, 1626, 1943, 1970, 2201
BstDSI CCRYGG 1 cut(s) 841
BstF5I GGATG 5 cut(s) 746, 1095, 1147, 1285, 2062
BstFNI CGCG 2 cut(s) 85, 502
BstH2I RGCGCY 1 cut(s) 284
BstHHI GCGC 3 cut(s) 85, 283, 1054
BstKTI GATC 9 cut(s) 183, 649, 673, 823, 840, 996, 1038, 2269, 2315
BstMAI GTCTC 7 cut(s) 298, 515, 584, 877, 1075, 1871, 2163
BstMBI GATC 9 cut(s) 180, 646, 670, 820, 837, 993, 1035, 2266, 2312
BstMCI CGRYCG 2 cut(s) 673, 1458
BstNI CCWGG 7 cut(s) 528, 922, 971, 1441, 1517, 1527, 1671
BstNSI RCATGY 3 cut(s) 83, 1323, 1618
BstSFI CTRYAG 3 cut(s) 139, 197, 1434
BstSLI GKGCMC 4 cut(s) 1136, 1221, 1272, 1388
BstUI CGCG 2 cut(s) 85, 502
BstV1I GCAGC 9 cut(s) 201, 535, 547, 604, 1168, 1212, 1420, 1436, 1448
BstV2I GAAGAC 1 cut(s) 540
BstX2I RGATCY 1 cut(s) 2266
BstXI CCANNNNNNTGG 4 cut(s) 794, 1121, 1418, 1746
BstYI RGATCY 1 cut(s) 2266
BsuI GTATCC 1 cut(s) 1777
BtgI CCRYGG 1 cut(s) 841
BtgZI GCGATG 1 cut(s) 517
BtrI CACGTC 2 cut(s) 163, 727
BtsCI GGATG 5 cut(s) 746, 1095, 1147, 1285, 2062
BtsI GCAGTG 3 cut(s) 194, 636, 1981
BtsIMutI CAGTG 4 cut(s) 194, 636, 1151, 1981
CaiI CAGNNNCTG 1 cut(s) 178
CfoI GCGC 3 cut(s) 85, 283, 1054
Cfr10I RCCGGY 5 cut(s) 154, 1019, 1310, 1802, 1830
Cfr9I CCCGGG 1 cut(s) 690
CseI GACGC 3 cut(s) 74, 280, 1232
CsiI ACCWGGT 1 cut(s) 1515
Csp6I GTAC 8 cut(s) 77, 439, 865, 1357, 1713, 1750, 1828, 1928
CspAI ACCGGT 1 cut(s) 1830
CspCI CAANNNNNGTGG 4 cut(s) 1708, 1743, 1982, 2017
CviQI GTAC 8 cut(s) 77, 439, 865, 1357, 1713, 1750, 1828, 1928
DdeI CTNAG 8 cut(s) 315, 552, 1428, 1560, 1626, 1943, 1970, 2201
DpnI GATC 9 cut(s) 182, 648, 672, 822, 839, 995, 1037, 2268, 2314
DpnII GATC 9 cut(s) 180, 646, 670, 820, 837, 993, 1035, 2266, 2312
EaeI YGGCCR 1 cut(s) 1791
Eam1104I CTCTTC 3 cut(s) 505, 2028, 2076
EarI CTCTTC 3 cut(s) 505, 2028, 2076
EciI GGCGGA 3 cut(s) 959, 1880, 2079
Eco130I CCWWGG 2 cut(s) 583, 1136
Eco147I AGGCCT 1 cut(s) 1565
Eco24I GRGCYC 3 cut(s) 476, 1136, 1272
Eco31I GGTCTC 3 cut(s) 584, 1871, 2163
Eco32I GATATC 1 cut(s) 1667
Eco47I GGWCC 6 cut(s) 157, 175, 967, 1509, 1844, 2140
Eco57I CTGAAG 1 cut(s) 2240
Eco88I CYCGRG 2 cut(s) 690, 1085
EcoO109I RGGNCCY 1 cut(s) 175
EcoRI GAATTC 1 cut(s) 2232
EcoRII CCWGG 7 cut(s) 526, 920, 969, 1439, 1515, 1525, 1669
EcoRV GATATC 1 cut(s) 1667
EcoT14I CCWWGG 2 cut(s) 583, 1136
EcoT38I GRGCYC 3 cut(s) 476, 1136, 1272
ErhI CCWWGG 2 cut(s) 583, 1136
Esp3I CGTCTC 1 cut(s) 298
FalI AAGNNNNNCTT 4 cut(s) 692, 724, 2077, 2109
FauI CCCGC 3 cut(s) 317, 507, 952
FauNDI CATATG 1 cut(s) 721
FblI GTMKAC 1 cut(s) 2211
FokI GGATG 5 cut(s) 753, 1102, 1154, 1292, 2069
FriOI GRGCYC 3 cut(s) 476, 1136, 1272
FseI GGCCGGCC 1 cut(s) 1023
GlaI GCGC 3 cut(s) 84, 282, 1053
GsuI CTGGAG 1 cut(s) 2291
HaeII RGCGCY 1 cut(s) 284
HgaI GACGC 3 cut(s) 74, 280, 1232
HhaI GCGC 3 cut(s) 85, 283, 1054
Hin1I GRCGYC 1 cut(s) 291
Hin6I GCGC 3 cut(s) 83, 281, 1052
HinP1I GCGC 3 cut(s) 83, 281, 1052
HincII GTYRAC 1 cut(s) 120
HindII GTYRAC 1 cut(s) 120
HindIII AAGCTT 2 cut(s) 23, 2252
HinfI GANTC 5 cut(s) 523, 884, 1083, 1986, 2224
HpaI GTTAAC 1 cut(s) 120
HphI GGTGA 4 cut(s) 232, 355, 1238, 2255
Hpy188I TCNGA 8 cut(s) 185, 277, 407, 421, 877, 1279, 2238, 2259
Hpy188III TCNNGA 9 cut(s) 173, 650, 1087, 1502, 1551, 1569, 1591, 1757, 2270
Hpy99I CGWCG 1 cut(s) 593
HpyAV CCTTC 6 cut(s) 41, 388, 746, 1870, 1897, 2103
HpyCH4III ACNGT 5 cut(s) 881, 1072, 1240, 1255, 1459
HpyCH4IV ACGT 4 cut(s) 162, 480, 726, 1826
HpyF3I CTNAG 8 cut(s) 315, 552, 1428, 1560, 1626, 1943, 1970, 2201
HpySE526I ACGT 4 cut(s) 162, 480, 726, 1826
Hsp92I GRCGYC 1 cut(s) 291
HspAI GCGC 3 cut(s) 83, 281, 1052
KpnI GGTACC 2 cut(s) 1753, 1931
KroI GCCGGC 2 cut(s) 1019, 1802
KroNI GCCGGC 2 cut(s) 1021, 1804
KspAI GTTAAC 1 cut(s) 120
Kzo9I GATC 9 cut(s) 180, 646, 670, 820, 837, 993, 1035, 2266, 2312
LguI GCTCTTC 1 cut(s) 2028
LmnI GCTCC 6 cut(s) 338, 471, 593, 1646, 2048, 2272
Lsp1109I GCAGC 9 cut(s) 201, 535, 547, 604, 1168, 1212, 1420, 1436, 1448
LweI GCATC 1 cut(s) 2284
MabI ACCWGGT 1 cut(s) 1515
MaeII ACGT 4 cut(s) 162, 480, 726, 1826
MaeIII GTNAC 5 cut(s) 11, 494, 881, 1244, 1282
MalI GATC 9 cut(s) 182, 648, 672, 822, 839, 995, 1037, 2268, 2314
MboI GATC 9 cut(s) 180, 646, 670, 820, 837, 993, 1035, 2266, 2312
MboII GAAGA 5 cut(s) 217, 522, 545, 2015, 2063
MfeI CAATTG 3 cut(s) 655, 1608, 2120
MflI RGATCY 1 cut(s) 2266
MhlI GDGCHC 5 cut(s) 476, 1136, 1221, 1272, 1388
MlsI TGGCCA 1 cut(s) 1793
MluNI TGGCCA 1 cut(s) 1793
MlyI GAGTC 4 cut(s) 517, 878, 1077, 1995
MmeI TCCRAC 3 cut(s) 255, 900, 1642
Mox20I TGGCCA 1 cut(s) 1793
MroNI GCCGGC 2 cut(s) 1019, 1802
MroXI GAANNNNTTC 1 cut(s) 488
MscI TGGCCA 1 cut(s) 1793
MseI TTAA 8 cut(s) 69, 119, 609, 684, 792, 954, 1874, 2037
MslI CAYNNNNRTG 4 cut(s) 236, 1793, 1919, 2031
Msp20I TGGCCA 1 cut(s) 1793
MspA1I CMGCKG 1 cut(s) 1427
MspCI CTTAAG 2 cut(s) 608, 1873
MunI CAATTG 3 cut(s) 655, 1608, 2120
Mva1269I GAATGC 1 cut(s) 252
MvaI CCWGG 7 cut(s) 528, 922, 971, 1441, 1517, 1527, 1671
MvnI CGCG 2 cut(s) 85, 502
NaeI GCCGGC 2 cut(s) 1021, 1804
NciI CCSGG 7 cut(s) 109, 309, 336, 691, 692, 1818, 2155
NdeI CATATG 1 cut(s) 721
NdeII GATC 9 cut(s) 180, 646, 670, 820, 837, 993, 1035, 2266, 2312
NgoMIV GCCGGC 2 cut(s) 1019, 1802
NheI GCTAGC 2 cut(s) 563, 1054
NmeAIII GCCGAG 2 cut(s) 267, 1848
NmuCI GTSAC 3 cut(s) 494, 881, 1244
NspI RCATGY 3 cut(s) 83, 1323, 1618
OliI CACNNNNGTG 1 cut(s) 1919
PceI AGGCCT 1 cut(s) 1565
PciI ACATGT 1 cut(s) 1614
PciSI GCTCTTC 1 cut(s) 2028
PcsI WCGNNNNNNNCGW 1 cut(s) 1092
PctI GAATGC 1 cut(s) 252
PdiI GCCGGC 2 cut(s) 1021, 1804
PdmI GAANNNNTTC 1 cut(s) 488
PfeI GAWTC 1 cut(s) 2224
PflFI GACNNNGTC 2 cut(s) 539, 1517
PflMI CCANNNNNTGG 3 cut(s) 1168, 1643, 1656
PfoI TCCNGGA 1 cut(s) 334
PinAI ACCGGT 1 cut(s) 1830
Ple19I CGATCG 1 cut(s) 673
PleI GAGTC 4 cut(s) 517, 878, 1077, 1994
PpsI GAGTC 4 cut(s) 517, 878, 1077, 1994
PpuMI RGGWCCY 1 cut(s) 175
PscI ACATGT 1 cut(s) 1614
Psp5II RGGWCCY 1 cut(s) 175
Psp6I CCWGG 7 cut(s) 526, 920, 969, 1439, 1515, 1525, 1669
PspGI CCWGG 7 cut(s) 526, 920, 969, 1439, 1515, 1525, 1669
PspOMI GGGCCC 2 cut(s) 1132, 1268
PspPPI RGGWCCY 1 cut(s) 175
PstI CTGCAG 3 cut(s) 143, 201, 1438
PstNI CAGNNNCTG 1 cut(s) 178
PsuI RGATCY 1 cut(s) 2266
PsyI GACNNNGTC 2 cut(s) 539, 1517
PvuI CGATCG 1 cut(s) 673
PvuII CAGCTG 1 cut(s) 1427
RigI GGCCGGCC 1 cut(s) 1023
RsaI GTAC 8 cut(s) 78, 440, 866, 1358, 1714, 1751, 1829, 1929
RsaNI GTAC 8 cut(s) 77, 439, 865, 1357, 1713, 1750, 1828, 1928
RseI CAYNNNNRTG 4 cut(s) 236, 1793, 1919, 2031
SapI GCTCTTC 1 cut(s) 2028
SaqAI TTAA 8 cut(s) 69, 119, 609, 684, 792, 954, 1874, 2037
Sau3AI GATC 9 cut(s) 180, 646, 670, 820, 837, 993, 1035, 2266, 2312
SchI GAGTC 4 cut(s) 517, 878, 1077, 1995
SduI GDGCHC 5 cut(s) 476, 1136, 1221, 1272, 1388
SexAI ACCWGGT 1 cut(s) 1515
SfaNI GCATC 1 cut(s) 2284
SfcI CTRYAG 3 cut(s) 139, 197, 1434
SinI GGWCC 6 cut(s) 157, 175, 967, 1509, 1844, 2140
SmaI CCCGGG 1 cut(s) 692
SmiMI CAYNNNNRTG 4 cut(s) 236, 1793, 1919, 2031
SmlI CTYRAG 4 cut(s) 608, 1755, 1873, 2083
SmoI CTYRAG 4 cut(s) 608, 1755, 1873, 2083
SseBI AGGCCT 1 cut(s) 1565
StuI AGGCCT 1 cut(s) 1565
StyI CCWWGG 2 cut(s) 583, 1136
TaaI ACNGT 5 cut(s) 881, 1072, 1240, 1255, 1459
TaiI ACGT 4 cut(s) 165, 483, 729, 1829
TaqI TCGA 5 cut(s) 651, 669, 1095, 1476, 1550
TaqII GACCGA 2 cut(s) 1526, 1853
TatI WGTACW 1 cut(s) 438
TauI GCSGC 3 cut(s) 374, 941, 944
TfiI GAWTC 1 cut(s) 2224
Tru1I TTAA 8 cut(s) 69, 119, 609, 684, 792, 954, 1874, 2037
Tru9I TTAA 8 cut(s) 69, 119, 609, 684, 792, 954, 1874, 2037
TscAI CASTG 4 cut(s) 201, 643, 1158, 1981
TseFI GTSAC 3 cut(s) 494, 881, 1244
TseI GCWGC 9 cut(s) 214, 548, 560, 617, 1181, 1225, 1424, 1433, 1436
Tsp45I GTSAC 3 cut(s) 494, 881, 1244
TspDTI ATGAA 4 cut(s) 438, 768, 1361, 2015
TspGWI ACGGA 5 cut(s) 482, 606, 830, 1016, 2275
TspMI CCCGGG 1 cut(s) 690
TspRI CASTG 4 cut(s) 201, 643, 1158, 1981
Tth111I GACNNNGTC 2 cut(s) 539, 1517
Van91I CCANNNNNTGG 3 cut(s) 1168, 1643, 1656
Vha464I CTTAAG 2 cut(s) 608, 1873
VneI GTGCAC 1 cut(s) 1384
VpaK11BI GGWCC 6 cut(s) 157, 175, 967, 1509, 1844, 2140
XapI RAATTY 2 cut(s) 1496, 2232
XbaI TCTAGA 1 cut(s) 1568
XceI RCATGY 3 cut(s) 83, 1323, 1618
XmaI CCCGGG 1 cut(s) 690
XmiI GTMKAC 1 cut(s) 2211
XmnI GAANNNNTTC 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.