RchiOBHm_Chr4g0424381

beta-D-xylosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0012799
Physical Location & Seq
Reverse (-)
49978829 .. 49983287
4459 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39365

Sequence Viewer

Length: 2331 bp
ATGGCTTCTGGTTACAATAATAAGCTTTCCCTTCTTGCTCTTGTTCTCTGTGTATCAGCATTGTTGCTCTGCAGCACGTTTAATCTGGTACATGCGCGTCCACCCTTTGCATGTGACCCTCGGAACCCGTTAACGAGAGGGTTCAAGTTCTGCAGGACGAGGGTGCCGGTCCACATGAGGGTTCAGGACCTGATCGGACGGCTGACATTGCAGGAGAAGATTAGGCTGCTGGTGAACAATGCCATAGCTGTGCCGAGGCTCGGCATTCAGGGATACGAGTGGTGGTCGGAGGCGCTTCACGGCGTCTCTAATGTGGGCCCCGGGACTAAGTTTGGTGGGGCATTTCCGGGCGCCACTAGCTTCCCTCAAGTCATCACCACCGCCGCTTCCTTCAATCAATCACTGTGGCAAGAAATCGGTCAGGTGGTGTCTGATGAAGCAAGAGCAATGTACAATGGAGGCCAGGCTGGTTTGACATATTGGAGCCCTAATGTGAACATATTCCGTGACCCGCGATGGGGAAGAGGACAAGAGACTCCTGGGGAAGACCCTGTTTTGGCTGCTAAGTATGCTGCTAGCTATGTCAAGGGTCTCCAAGGTGATGGAGCTGGAAACCGGCTTAAGGTTGCGGCATGTTGTAAGCATTACACGGCCTATGATCTTGACAATTGGAATGGCGTCGATCGCTTCCATTTCAATGCCAGGGTTAGCAAGCAGGACTTGGCAGACACATATGACGTTCCGTTTAGAGGCTGTGTGTTAGAAGGAAAGGTTGCTAGTGTCATGTGCTCATACAACCAAGTCAATGGAAAGCCTACTTGTGCTGACCCTGATCTCCTAAAGAACACAATCCGTGGCCAATGGAAACTCAATGGGTACATAGTCTCCGACTGTGACTCGGTGGGAGTCTTGTATGATGAGCAACATTACACCAAGACACCAGAAGAAGCCGCGGCGGACGCGATCAAAGCGGGTTTGGATCTGGACTGTGGGCCGTTTCTCGCAATCCATACGGAGGGTGCTATAAAGTCCGGCCTGCTACAAGAGATCGACGTGAACTATGCGCTAGCAAACACTCTTACGGTCCAAATGAGACTCGGGATGTTCGATGGCGAACCATCGGCCCAACAATACGGAAACTTGGGCCCAAGAGATGTTTGCACCCCGGCCCACCAACAGTTGGCACTTGAGGCCGCTAGGCAAGGCATTGTCCTGCTTCAAAACAATCGGCATTCACTGCCTCTCTCGACTGTGCGTCACCATACCATAGCGGTAATTGGGCCCAACTCGGATGTTACCGTCACGATGATAGGAAATTATGCCGGTGTTGCATGTGGCTACACAACTCCCTTGCAAGGAATTGGGAGGTACACAAAGACCATTCATCAACAAGGGTGCACCGATGTTGCTTGCACTGGTAACCAACTATTTAGGGCAGCTGAGGCTGCAGCCTGGCAGGCCGATGCGACCGTCTTGGTTATGGGCCTCGACCAATCTATCGAAGCTGAATTCCGGGATAGGACCGGGCTGCTCTTACCGGGACACCAACAAGAGCTCGTGTCGAGAGTGGCTCAGGCCTCTAGAGGCCCAACCATATTGGTCTTGATGTCTGGTGGCCCAATTGATGTATCGTTTGCGAAGAACGACCCAAAAATTGGAGCCATAATTTGGGTTGGATATCCTGGTCAAGCAGGAGGAACTGCGATTGCTGATGTTCTGTTTGGAACTACTAATCCAGGTGGGAAGCTACCGATGACATGGTACCCCCAAGACTATGTATCCAAAGTGCCCATGACAAACATGGCCATGCGTCCTGCCAGAGGCTACCCCGGTAGGACATACCGGTTCTACAAAGGTCCTGTGGTATTTCCCTTCGGTCTCGGCTTAAGCTATACCACCTTTGCCCACTCACTAGCCCAAGTTCCCACATTGGTTTCGGTACGCCTCACAAGTCTAAGTGCCACCACAAACTCAACCATGCTAAGCAGTGCTGTGAGAGTCAGCCACACAAATTGCAATTCGCTCTCACTTCCTGTTCATGTTGATGTTAAAAACACCGGAGCTAGGGATGGAACTCACACTCTTCTAGTATTCTCGAGCCCGCCTTTGGGGAAATGGGCCGCGAACAAGCAATTGGTGGGCTTTCACAAGGTCCATATTTTGGCCGGATCTCAGAAACGGGTCAAGGTTGATGTTCATGTTTGCAAGCACCTCAGTGTTGTTGACCAGTTTGGCATTCGAAGAATTCCGATTGGTGAACATAAGCTTCAGATTGGAGATTTGGAGCATCACATCTCCATTGAAGCCAATTTAGGAGATATTATATCTTAA

Protein Analysis

776

Amino Acids

84.03

Weight (kDa)

8.68

Isoelectric Point (pI)

31.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_3 PF00933 115 - 330 1.5e-34 Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C PF01915 403 - 631 6.4e-51 Glycosyl hydrolase family 3 C-terminal domain
Fn3-like PF14310 697 - 760 3e-08 Fibronectin type III-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012799)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49360
fragaria_vesca FvH4_4g19350
malus_domestica MD13G1157400.v1.1 MD16G1158300.v1.1
prunus_persica Prupe.1G123100_v2.0.a1
pyrus_communis pycom16g13320
rosa_chinensis RchiOBHm_Chr4g0424381
rosa_laevigata RLG00000007479
rosa_multiflora Rmu_co8330357.1_g000001 Rmu_sc0003567.1_g000021 Rmu_sc0005995.1_g000003
rosa_roxburghii Rroxscaffold_5G00366330
rosa_rugosa Rorug04G0197100 Rorug04G0197200
rosa_samantha Rh4AG253200 Rh4CG269900 Rh4DG253000
rosa_wichuraiana Rw4G021850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1761
AccB1I GGYRCC 3 cut(s) 163, 350, 1761
AccB7I CCANNNNNTGG 4 cut(s) 1180, 1655, 1668, 2161
AccII CGCG 5 cut(s) 97, 514, 953, 962, 2123
AclWI GGATC 2 cut(s) 987, 2176
AcoI YGGCCR 3 cut(s) 856, 1803, 2163
AcsI RAATTY 2 cut(s) 1508, 2244
AcuI CTGAAG 1 cut(s) 2252
AcyI GRCGYC 3 cut(s) 303, 351, 678
AfaI GTAC 6 cut(s) 90, 452, 878, 1370, 1763, 1941
AflII CTTAAG 2 cut(s) 620, 1885
AgeI ACCGGT 1 cut(s) 1842
AgsI TTSAA 5 cut(s) 145, 394, 697, 1220, 2303
AhdI GACNNNNNGTC 1 cut(s) 1254
AjiI CACGTC 1 cut(s) 1054
AjnI CCWGG 6 cut(s) 462, 538, 701, 1451, 1681, 1735
AjuI GAANNNNNNNTTGG 2 cut(s) 2117, 2149
AleI CACNNNNGTG 1 cut(s) 2214
AloI GAACNNNNNNTCC 2 cut(s) 1717, 1749
Alw21I GWGCWC 3 cut(s) 791, 1400, 1557
Alw26I GTCTC 6 cut(s) 310, 527, 596, 889, 1087, 1883
Alw44I GTGCAC 1 cut(s) 1396
AlwI GGATC 2 cut(s) 987, 2176
AlwNI CAGNNNCTG 1 cut(s) 190
Ama87I CYCGRG 3 cut(s) 320, 1097, 2095
ApaI GGGCCC 3 cut(s) 320, 1148, 1284
ApaLI GTGCAC 1 cut(s) 1396
ApeKI GCWGC 8 cut(s) 72, 226, 560, 572, 1436, 1445, 1448, 1528
ApoI RAATTY 2 cut(s) 1508, 2244
AsiGI ACCGGT 1 cut(s) 1842
Asp700I GAANNNNTTC 1 cut(s) 500
Asp718I GGTACC 1 cut(s) 1761
AspLEI GCGC 4 cut(s) 97, 295, 353, 1066
AsuC2I CCSGG 8 cut(s) 321, 322, 348, 1166, 1514, 1525, 1539, 1830
AsuHPI GGTGA 5 cut(s) 244, 367, 611, 1250, 2267
AsuII TTCGAA 1 cut(s) 2239
AsuNHI GCTAGC 2 cut(s) 575, 1066
AvaI CYCGRG 3 cut(s) 320, 1097, 2095
AvaII GGWCC 6 cut(s) 169, 187, 1084, 1521, 1856, 2152
BaeGI GKGCMC 5 cut(s) 320, 1148, 1284, 1400, 1791
BalI TGGCCA 2 cut(s) 858, 1805
BanI GGYRCC 3 cut(s) 163, 350, 1761
BanII GRGCYC 6 cut(s) 320, 488, 1148, 1284, 1557, 2102
BauI CACGAG 1 cut(s) 1556
BbsI GAAGAC 1 cut(s) 552
Bbv12I GWGCWC 3 cut(s) 791, 1400, 1557
BbvCI CCTCAGC 1 cut(s) 1440
BbvI GCAGC 8 cut(s) 84, 213, 547, 559, 1432, 1448, 1460, 1515
BccI CCATC 5 cut(s) 510, 596, 1103, 1126, 2063
BceAI ACGGC 4 cut(s) 215, 316, 666, 979
BciT130I CCWGG 6 cut(s) 464, 540, 703, 1453, 1683, 1737
BciVI GTATCC 2 cut(s) 266, 1789
BcnI CCSGG 8 cut(s) 321, 322, 348, 1166, 1514, 1525, 1539, 1830
BcoDI GTCTC 6 cut(s) 310, 527, 596, 889, 1087, 1883
BfaI CTAG 9 cut(s) 357, 576, 777, 1067, 1197, 1581, 1913, 2064, 2087
BfmI CTRYAG 3 cut(s) 70, 151, 1446
BfoI RGCGCY 2 cut(s) 296, 354
BfrI CTTAAG 2 cut(s) 620, 1885
BfuI GTATCC 2 cut(s) 266, 1789
BglI GCCNNNNNGGC 1 cut(s) 1457
BlpI GCTNAGC 1 cut(s) 1982
Bme18I GGWCC 6 cut(s) 169, 187, 1084, 1521, 1856, 2152
BmeRI GACNNNNNGTC 1 cut(s) 1254
BmeT110I CYCGRG 3 cut(s) 320, 1097, 2095
BmgBI CACGTC 1 cut(s) 1054
BmsI GCATC 2 cut(s) 1453, 2296
BmtI GCTAGC 2 cut(s) 579, 1070
BpiI GAAGAC 1 cut(s) 552
BplI GAGNNNNNCTC 2 cut(s) 1555, 1587
Bpu10I CCTNAGC 2 cut(s) 1440, 1572
Bpu1102I GCTNAGC 1 cut(s) 1982
Bpu14I TTCGAA 1 cut(s) 2239
BpuEI CTTGAG 2 cut(s) 351, 1208
BpuMI CCSGG 8 cut(s) 321, 322, 348, 1166, 1514, 1525, 1539, 1830
BsaHI GRCGYC 3 cut(s) 303, 351, 678
BsaI GGTCTC 2 cut(s) 596, 1883
BsaWI WCCGGW 2 cut(s) 1842, 2057
BsaXI ACNNNNNCTCC 2 cut(s) 869, 899
Bse118I RCCGGY 4 cut(s) 166, 615, 1322, 1842
Bse1I ACTGG 2 cut(s) 1420, 2227
Bse3DI GCAATG 2 cut(s) 206, 453
BseBI CCWGG 6 cut(s) 464, 540, 703, 1453, 1683, 1737
BseGI GGATG 3 cut(s) 1107, 1297, 2074
BseMI GCAATG 2 cut(s) 206, 453
BseMII CTCAG 4 cut(s) 1431, 1586, 2186, 2227
BseNI ACTGG 2 cut(s) 1420, 2227
BseSI GKGCMC 5 cut(s) 320, 1148, 1284, 1400, 1791
BseXI GCAGC 8 cut(s) 84, 213, 547, 559, 1432, 1448, 1460, 1515
Bsh1236I CGCG 5 cut(s) 97, 514, 953, 962, 2123
Bsh1285I CGRYCG 2 cut(s) 685, 1470
BshNI GGYRCC 3 cut(s) 163, 350, 1761
BshTI ACCGGT 1 cut(s) 1842
BsiEI CGRYCG 2 cut(s) 685, 1470
BsiHKAI GWGCWC 3 cut(s) 791, 1400, 1557
BsiHKCI CYCGRG 3 cut(s) 320, 1097, 2095
BslFI GGGAC 2 cut(s) 337, 1554
BsmAI GTCTC 6 cut(s) 310, 527, 596, 889, 1087, 1883
BsmBI CGTCTC 1 cut(s) 310
BsmFI GGGAC 2 cut(s) 337, 1554
BsmI GAATGC 3 cut(s) 264, 1231, 2235
Bso31I GGTCTC 2 cut(s) 596, 1883
BsoBI CYCGRG 3 cut(s) 320, 1097, 2095
Bsp119I TTCGAA 1 cut(s) 2239
Bsp120I GGGCCC 3 cut(s) 316, 1144, 1280
Bsp1286I GDGCHC 9 cut(s) 320, 488, 791, 1148, 1284, 1400, 1557, 1791, 2102
Bsp1407I TGTACA 1 cut(s) 450
Bsp143I GATC 8 cut(s) 192, 658, 682, 832, 963, 979, 1047, 2168
Bsp1720I GCTNAGC 1 cut(s) 1982
BspCNI CTCAG 4 cut(s) 1432, 1585, 2185, 2226
BspFNI CGCG 5 cut(s) 97, 514, 953, 962, 2123
BspMAI CTGCAG 3 cut(s) 74, 155, 1450
BspOI GCTAGC 2 cut(s) 579, 1070
BspPI GGATC 2 cut(s) 987, 2176
BspT104I TTCGAA 1 cut(s) 2239
BspT107I GGYRCC 3 cut(s) 163, 350, 1761
BspTI CTTAAG 2 cut(s) 620, 1885
BspTNI GGTCTC 2 cut(s) 596, 1883
BsrDI GCAATG 2 cut(s) 206, 453
BsrFI RCCGGY 4 cut(s) 166, 615, 1322, 1842
BsrGI TGTACA 1 cut(s) 450
BsrI ACTGG 2 cut(s) 1420, 2227
BssAI RCCGGY 4 cut(s) 166, 615, 1322, 1842
BssMI GATC 8 cut(s) 192, 658, 682, 832, 963, 979, 1047, 2168
BssNI GRCGYC 3 cut(s) 303, 351, 678
BssSI CACGAG 1 cut(s) 1556
BssT1I CCWWGG 1 cut(s) 595
Bst2BI CACGAG 1 cut(s) 1556
Bst2UI CCWGG 6 cut(s) 464, 540, 703, 1453, 1683, 1737
Bst4CI ACNGT 8 cut(s) 405, 893, 989, 1084, 1179, 1252, 1300, 1471
Bst6I CTCTTC 2 cut(s) 517, 2088
BstACI GRCGYC 3 cut(s) 303, 351, 678
BstAFI CTTAAG 2 cut(s) 620, 1885
BstAPI GCANNNNNTGC 1 cut(s) 1237
BstAUI TGTACA 1 cut(s) 450
BstBI TTCGAA 1 cut(s) 2239
BstC8I GCNNGC 8 cut(s) 577, 713, 1037, 1068, 1411, 1458, 2102, 2207
BstDEI CTNAG 8 cut(s) 327, 564, 1440, 1572, 1955, 1982, 2172, 2213
BstDSI CCRYGG 2 cut(s) 853, 951
BstEII GGTNACC 1 cut(s) 1418
BstENI CCTNNNNNAGG 1 cut(s) 1818
BstF5I GGATG 3 cut(s) 1107, 1297, 2074
BstFNI CGCG 5 cut(s) 97, 514, 953, 962, 2123
BstH2I RGCGCY 2 cut(s) 296, 354
BstHHI GCGC 4 cut(s) 97, 295, 353, 1066
BstKTI GATC 8 cut(s) 195, 661, 685, 835, 966, 982, 1050, 2171
BstMAI GTCTC 6 cut(s) 310, 527, 596, 889, 1087, 1883
BstMBI GATC 8 cut(s) 192, 658, 682, 832, 963, 979, 1047, 2168
BstMCI CGRYCG 2 cut(s) 685, 1470
BstNI CCWGG 6 cut(s) 464, 540, 703, 1453, 1683, 1737
BstNSI RCATGY 4 cut(s) 95, 114, 636, 1335
BstPI GGTNACC 1 cut(s) 1418
BstSFI CTRYAG 3 cut(s) 70, 151, 1446
BstSLI GKGCMC 5 cut(s) 320, 1148, 1284, 1400, 1791
BstUI CGCG 5 cut(s) 97, 514, 953, 962, 2123
BstV1I GCAGC 8 cut(s) 84, 213, 547, 559, 1432, 1448, 1460, 1515
BstV2I GAAGAC 1 cut(s) 552
BstX2I RGATCY 2 cut(s) 979, 2168
BstXI CCANNNNNNTGG 2 cut(s) 602, 806
BstYI RGATCY 2 cut(s) 979, 2168
BsuI GTATCC 2 cut(s) 266, 1789
BtgI CCRYGG 2 cut(s) 853, 951
BtgZI GCGATG 1 cut(s) 529
BtrI CACGTC 1 cut(s) 1054
BtsCI GGATG 3 cut(s) 1107, 1297, 2074
BtsI GCAGTG 2 cut(s) 1235, 1993
BtsIMutI CAGTG 5 cut(s) 401, 1235, 1413, 1993, 2221
Cac8I GCNNGC 8 cut(s) 577, 713, 1037, 1068, 1411, 1458, 2102, 2207
CaiI CAGNNNCTG 1 cut(s) 190
CfoI GCGC 4 cut(s) 97, 295, 353, 1066
Cfr10I RCCGGY 4 cut(s) 166, 615, 1322, 1842
Cfr42I CCGCGG 1 cut(s) 954
Cfr9I CCCGGG 1 cut(s) 320
CseI GACGC 6 cut(s) 86, 292, 667, 968, 1244, 1799
Csp6I GTAC 6 cut(s) 89, 451, 877, 1369, 1762, 1940
CspAI ACCGGT 1 cut(s) 1842
CspCI CAANNNNNGTGG 4 cut(s) 386, 421, 1994, 2029
CviQI GTAC 6 cut(s) 89, 451, 877, 1369, 1762, 1940
DdeI CTNAG 8 cut(s) 327, 564, 1440, 1572, 1955, 1982, 2172, 2213
DinI GGCGCC 1 cut(s) 352
DpnI GATC 8 cut(s) 194, 660, 684, 834, 965, 981, 1049, 2170
DpnII GATC 8 cut(s) 192, 658, 682, 832, 963, 979, 1047, 2168
DriI GACNNNNNGTC 1 cut(s) 1254
EaeI YGGCCR 3 cut(s) 856, 1803, 2163
Eam1104I CTCTTC 2 cut(s) 517, 2088
Eam1105I GACNNNNNGTC 1 cut(s) 1254
EarI CTCTTC 2 cut(s) 517, 2088
EciI GGCGGA 1 cut(s) 971
Ecl136II GAGCTC 1 cut(s) 1555
Eco130I CCWWGG 1 cut(s) 595
Eco147I AGGCCT 1 cut(s) 1577
Eco24I GRGCYC 6 cut(s) 320, 488, 1148, 1284, 1557, 2102
Eco31I GGTCTC 2 cut(s) 596, 1883
Eco32I GATATC 1 cut(s) 1679
Eco47I GGWCC 6 cut(s) 169, 187, 1084, 1521, 1856, 2152
Eco53kI GAGCTC 1 cut(s) 1555
Eco57I CTGAAG 1 cut(s) 2252
Eco88I CYCGRG 3 cut(s) 320, 1097, 2095
Eco91I GGTNACC 1 cut(s) 1418
EcoICRI GAGCTC 1 cut(s) 1555
EcoNI CCTNNNNNAGG 1 cut(s) 1818
EcoO109I RGGNCCY 3 cut(s) 187, 317, 1856
EcoO65I GGTNACC 1 cut(s) 1418
EcoRI GAATTC 2 cut(s) 1508, 2244
EcoRII CCWGG 6 cut(s) 462, 538, 701, 1451, 1681, 1735
EcoRV GATATC 1 cut(s) 1679
EcoT14I CCWWGG 1 cut(s) 595
EcoT38I GRGCYC 6 cut(s) 320, 488, 1148, 1284, 1557, 2102
EgeI GGCGCC 1 cut(s) 352
EheI GGCGCC 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 595
Esp3I CGTCTC 1 cut(s) 310
FalI AAGNNNNNCTT 2 cut(s) 704, 736
FaqI GGGAC 2 cut(s) 337, 1554
FauI CCCGC 3 cut(s) 519, 964, 2109
FauNDI CATATG 1 cut(s) 733
FokI GGATG 3 cut(s) 1114, 1304, 2081
FriOI GRGCYC 6 cut(s) 320, 488, 1148, 1284, 1557, 2102
FspBI CTAG 9 cut(s) 357, 576, 777, 1067, 1197, 1581, 1913, 2064, 2087
GlaI GCGC 4 cut(s) 96, 294, 352, 1065
HaeII RGCGCY 2 cut(s) 296, 354
HgaI GACGC 6 cut(s) 86, 292, 667, 968, 1244, 1799
HhaI GCGC 4 cut(s) 97, 295, 353, 1066
Hin1I GRCGYC 3 cut(s) 303, 351, 678
Hin6I GCGC 4 cut(s) 95, 293, 351, 1064
HinP1I GCGC 4 cut(s) 95, 293, 351, 1064
HincII GTYRAC 2 cut(s) 132, 2224
HindII GTYRAC 2 cut(s) 132, 2224
HindIII AAGCTT 2 cut(s) 23, 2264
HinfI GANTC 5 cut(s) 535, 896, 906, 1095, 1998
HpaI GTTAAC 1 cut(s) 132
HphI GGTGA 5 cut(s) 244, 367, 611, 1250, 2267
Hpy188I TCNGA 9 cut(s) 123, 197, 289, 433, 889, 1291, 2175, 2250, 2271
Hpy99I CGWCG 2 cut(s) 683, 1055
HpyAV CCTTC 4 cut(s) 41, 400, 758, 1882
HpyCH4III ACNGT 8 cut(s) 405, 893, 989, 1084, 1179, 1252, 1300, 1471
HpyCH4IV ACGT 3 cut(s) 77, 738, 1053
HpyF3I CTNAG 8 cut(s) 327, 564, 1440, 1572, 1955, 1982, 2172, 2213
HpySE526I ACGT 3 cut(s) 77, 738, 1053
Hsp92I GRCGYC 3 cut(s) 303, 351, 678
HspAI GCGC 4 cut(s) 95, 293, 351, 1064
KasI GGCGCC 1 cut(s) 350
KpnI GGTACC 1 cut(s) 1765
KspAI GTTAAC 1 cut(s) 132
KspI CCGCGG 1 cut(s) 954
Kzo9I GATC 8 cut(s) 192, 658, 682, 832, 963, 979, 1047, 2168
LmnI GCTCC 5 cut(s) 483, 605, 1658, 2060, 2284
Lsp1109I GCAGC 8 cut(s) 84, 213, 547, 559, 1432, 1448, 1460, 1515
LweI GCATC 2 cut(s) 1453, 2296
MaeI CTAG 9 cut(s) 357, 576, 777, 1067, 1197, 1581, 1913, 2064, 2087
MaeII ACGT 3 cut(s) 77, 738, 1053
MaeIII GTNAC 8 cut(s) 11, 113, 506, 893, 1256, 1294, 1300, 1418
MalI GATC 8 cut(s) 194, 660, 684, 834, 965, 981, 1049, 2170
MboI GATC 8 cut(s) 192, 658, 682, 832, 963, 979, 1047, 2168
MboII GAAGA 7 cut(s) 229, 534, 557, 956, 1651, 2075, 2253
MfeI CAATTG 3 cut(s) 667, 1620, 2132
MflI RGATCY 2 cut(s) 979, 2168
MhlI GDGCHC 9 cut(s) 320, 488, 791, 1148, 1284, 1400, 1557, 1791, 2102
MlsI TGGCCA 2 cut(s) 858, 1805
MluNI TGGCCA 2 cut(s) 858, 1805
Mly113I GGCGCC 1 cut(s) 351
MlyI GAGTC 5 cut(s) 529, 890, 915, 1089, 2007
MmeI TCCRAC 3 cut(s) 267, 912, 1654
Mox20I TGGCCA 2 cut(s) 858, 1805
MroXI GAANNNNTTC 1 cut(s) 500
MscI TGGCCA 2 cut(s) 858, 1805
MseI TTAA 6 cut(s) 81, 131, 621, 1886, 2049, 2329
MslI CAYNNNNRTG 5 cut(s) 248, 696, 1805, 2043, 2214
Msp20I TGGCCA 2 cut(s) 858, 1805
MspA1I CMGCKG 2 cut(s) 953, 1439
MspCI CTTAAG 2 cut(s) 620, 1885
MunI CAATTG 3 cut(s) 667, 1620, 2132
Mva1269I GAATGC 3 cut(s) 264, 1231, 2235
MvaI CCWGG 6 cut(s) 464, 540, 703, 1453, 1683, 1737
MvnI CGCG 5 cut(s) 97, 514, 953, 962, 2123
NarI GGCGCC 1 cut(s) 351
NciI CCSGG 8 cut(s) 321, 322, 348, 1166, 1514, 1525, 1539, 1830
NdeI CATATG 1 cut(s) 733
NdeII GATC 8 cut(s) 192, 658, 682, 832, 963, 979, 1047, 2168
NheI GCTAGC 2 cut(s) 575, 1066
NmeAIII GCCGAG 3 cut(s) 240, 279, 1860
NmuCI GTSAC 5 cut(s) 113, 506, 893, 1256, 1300
NspI RCATGY 4 cut(s) 95, 114, 636, 1335
NspV TTCGAA 1 cut(s) 2239
OliI CACNNNNGTG 1 cut(s) 2214
PaeR7I CTCGAG 1 cut(s) 2095
PceI AGGCCT 1 cut(s) 1577
PcsI WCGNNNNNNNCGW 1 cut(s) 1104
PctI GAATGC 3 cut(s) 264, 1231, 2235
PdmI GAANNNNTTC 1 cut(s) 500
PflMI CCANNNNNTGG 4 cut(s) 1180, 1655, 1668, 2161
PfoI TCCNGGA 1 cut(s) 1512
PinAI ACCGGT 1 cut(s) 1842
Ple19I CGATCG 1 cut(s) 685
PleI GAGTC 5 cut(s) 529, 890, 914, 1089, 2006
PluTI GGCGCC 1 cut(s) 354
PpsI GAGTC 5 cut(s) 529, 890, 914, 1089, 2006
PpuMI RGGWCCY 2 cut(s) 187, 1856
Psp124BI GAGCTC 1 cut(s) 1557
Psp5II RGGWCCY 2 cut(s) 187, 1856
Psp6I CCWGG 6 cut(s) 462, 538, 701, 1451, 1681, 1735
PspEI GGTNACC 1 cut(s) 1418
PspGI CCWGG 6 cut(s) 462, 538, 701, 1451, 1681, 1735
PspOMI GGGCCC 3 cut(s) 316, 1144, 1280
PspPPI RGGWCCY 2 cut(s) 187, 1856
PstI CTGCAG 3 cut(s) 74, 155, 1450
PstNI CAGNNNCTG 1 cut(s) 190
PsuI RGATCY 2 cut(s) 979, 2168
PvuI CGATCG 1 cut(s) 685
PvuII CAGCTG 1 cut(s) 1439
RsaI GTAC 6 cut(s) 90, 452, 878, 1370, 1763, 1941
RsaNI GTAC 6 cut(s) 89, 451, 877, 1369, 1762, 1940
RseI CAYNNNNRTG 5 cut(s) 248, 696, 1805, 2043, 2214
SacI GAGCTC 1 cut(s) 1557
SacII CCGCGG 1 cut(s) 954
SaqAI TTAA 6 cut(s) 81, 131, 621, 1886, 2049, 2329
Sau3AI GATC 8 cut(s) 192, 658, 682, 832, 963, 979, 1047, 2168
SchI GAGTC 5 cut(s) 529, 890, 915, 1089, 2007
SduI GDGCHC 9 cut(s) 320, 488, 791, 1148, 1284, 1400, 1557, 1791, 2102
SfaNI GCATC 2 cut(s) 1453, 2296
SfcI CTRYAG 3 cut(s) 70, 151, 1446
SfoI GGCGCC 1 cut(s) 352
Sfr274I CTCGAG 1 cut(s) 2095
Sfr303I CCGCGG 1 cut(s) 954
SfuI TTCGAA 1 cut(s) 2239
SgrBI CCGCGG 1 cut(s) 954
SinI GGWCC 6 cut(s) 169, 187, 1084, 1521, 1856, 2152
SlaI CTCGAG 1 cut(s) 2095
SmaI CCCGGG 1 cut(s) 322
SmiMI CAYNNNNRTG 5 cut(s) 248, 696, 1805, 2043, 2214
SmlI CTYRAG 5 cut(s) 366, 620, 1187, 1885, 2095
SmoI CTYRAG 5 cut(s) 366, 620, 1187, 1885, 2095
SseBI AGGCCT 1 cut(s) 1577
SspDI GGCGCC 1 cut(s) 350
SspMI CTAG 9 cut(s) 357, 576, 777, 1067, 1197, 1581, 1913, 2064, 2087
SstI GAGCTC 1 cut(s) 1557
StuI AGGCCT 1 cut(s) 1577
StyI CCWWGG 1 cut(s) 595
TaaI ACNGT 8 cut(s) 405, 893, 989, 1084, 1179, 1252, 1300, 1471
TaiI ACGT 3 cut(s) 80, 741, 1056
TaqI TCGA 9 cut(s) 681, 1050, 1107, 1247, 1488, 1500, 1562, 2096, 2239
TaqII GACCGA 2 cut(s) 407, 1865
TatI WGTACW 1 cut(s) 450
TauI GCSGC 6 cut(s) 386, 632, 953, 956, 1196, 2123
Tru1I TTAA 6 cut(s) 81, 131, 621, 1886, 2049, 2329
Tru9I TTAA 6 cut(s) 81, 131, 621, 1886, 2049, 2329
TscAI CASTG 5 cut(s) 408, 1242, 1420, 1993, 2221
TseFI GTSAC 5 cut(s) 113, 506, 893, 1256, 1300
TseI GCWGC 8 cut(s) 72, 226, 560, 572, 1436, 1445, 1448, 1528
Tsp45I GTSAC 5 cut(s) 113, 506, 893, 1256, 1300
TspDTI ATGAA 4 cut(s) 450, 1373, 2027, 2186
TspGWI ACGGA 5 cut(s) 494, 732, 842, 1028, 1149
TspMI CCCGGG 1 cut(s) 320
TspRI CASTG 5 cut(s) 408, 1242, 1420, 1993, 2221
Van91I CCANNNNNTGG 4 cut(s) 1180, 1655, 1668, 2161
Vha464I CTTAAG 2 cut(s) 620, 1885
VneI GTGCAC 1 cut(s) 1396
VpaK11BI GGWCC 6 cut(s) 169, 187, 1084, 1521, 1856, 2152
XagI CCTNNNNNAGG 1 cut(s) 1818
XapI RAATTY 2 cut(s) 1508, 2244
XbaI TCTAGA 1 cut(s) 1580
XceI RCATGY 4 cut(s) 95, 114, 636, 1335
XcmI CCANNNNNNNNNTGG 1 cut(s) 1798
XhoI CTCGAG 1 cut(s) 2095
XmaI CCCGGG 1 cut(s) 320
XmnI GAANNNNTTC 1 cut(s) 500
XspI CTAG 9 cut(s) 357, 576, 777, 1067, 1197, 1581, 1913, 2064, 2087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.