Rmu_co8330357.1_g000001

beta-D-xylosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8330357.1
OG0012799
Physical Location & Seq
Forward (+)
1 .. 949
949 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8330357.1_g000001.1.cds

Sequence Viewer

Length: 846 bp
ctcgaccaatctatcgaagctgaattcagggataggactgggctgctcttgccgggacaccaacaagagctcgtgtcgagagtggctcaggcctctagaggcccaaccatattggtcttgatgtctggtggcccaattgatgtgtcgtttgcgaagaacgacccaaaaattggagccataatttgggttggatatcctggtcaagcaggaggaactgcaattgctgatgttctgtttgggactaccaatccaggtgggaagctaccgatgacatggtacccccaagactatgtatccaaagtgcccatgacaaacatggccatgcgtcccgccagaggctaccccggtaggacgtaccgattctacaaaggtcccgtggtatttcccttcggtctcggcttaagctacaccacctttgcccactcactagcccaaggtcccacatcgatttcggtgcgcctcacaagtctaagtgccaccacaaactcaaccatgctaagcagtgctgtgagagccagccacacaaattgtaattctctctcacttcctgttcatgttgatgttaaaaacatcggagctagggatggaactcacactcttctagtattctcgagcccgcctttggggaaatgggccgcaaacaagcaattggtgggctttcacaaggtccatattctggccggatctcagaaacgggtcaaggttgatgttcatgtttgcaagcacctcagtgttgttgaccagtttggcattcgaagaattccgattggtaaacataagcttcagattggagatctggagcatcacatctccattgaagccaatttaggagatattatatcttaa

Protein Analysis

281

Amino Acids

30.31

Weight (kDa)

9.81

Isoelectric Point (pI)

33.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012799)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49360
fragaria_vesca FvH4_4g19350
malus_domestica MD13G1157400.v1.1 MD16G1158300.v1.1
prunus_persica Prupe.1G123100_v2.0.a1
pyrus_communis pycom16g13320
rosa_chinensis RchiOBHm_Chr4g0424381
rosa_laevigata RLG00000007479
rosa_multiflora Rmu_co8330357.1_g000001 Rmu_sc0003567.1_g000021 Rmu_sc0005995.1_g000003
rosa_roxburghii Rroxscaffold_5G00366330
rosa_rugosa Rorug04G0197100 Rorug04G0197200
rosa_samantha Rh4AG253200 Rh4CG269900 Rh4DG253000
rosa_wichuraiana Rw4G021850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 276
AccB1I GGYRCC 1 cut(s) 276
AccB7I CCANNNNNTGG 3 cut(s) 170, 183, 676
AciI CCGC 3 cut(s) 330, 617, 636
AclWI GGATC 1 cut(s) 691
AcoI YGGCCR 2 cut(s) 318, 678
AcsI RAATTY 2 cut(s) 23, 759
AcuI CTGAAG 1 cut(s) 767
AfaI GTAC 2 cut(s) 278, 356
AfiI CCNNNNNNNGG 6 cut(s) 170, 183, 335, 622, 623, 676
AflII CTTAAG 1 cut(s) 400
AgsI TTSAA 1 cut(s) 818
AjnI CCWGG 2 cut(s) 196, 250
AleI CACNNNNGTG 1 cut(s) 729
AluBI AGCT 6 cut(s) 20, 70, 262, 405, 578, 781
AluI AGCT 6 cut(s) 20, 70, 262, 405, 578, 781
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 1 cut(s) 691
Ama87I CYCGRG 1 cut(s) 610
AoxI GGCC 6 cut(s) 90, 100, 130, 318, 633, 678
ApeKI GCWGC 1 cut(s) 43
ApoI RAATTY 2 cut(s) 23, 759
Asp718I GGTACC 1 cut(s) 276
AspLEI GCGC 1 cut(s) 459
AspS9I GGNCC 6 cut(s) 101, 131, 371, 437, 633, 667
AsuC2I CCSGG 2 cut(s) 54, 345
AsuII TTCGAA 1 cut(s) 754
AvaI CYCGRG 1 cut(s) 610
AvaII GGWCC 3 cut(s) 371, 437, 667
BaeGI GKGCMC 1 cut(s) 306
BalI TGGCCA 1 cut(s) 320
BanI GGYRCC 1 cut(s) 276
BanII GRGCYC 2 cut(s) 72, 617
BauI CACGAG 1 cut(s) 71
Bbv12I GWGCWC 1 cut(s) 72
BbvI GCAGC 1 cut(s) 30
BccI CCATC 1 cut(s) 578
BcgI CGANNNNNNTGC 2 cut(s) 436, 470
BciT130I CCWGG 2 cut(s) 198, 252
BciVI GTATCC 1 cut(s) 304
BcnI CCSGG 2 cut(s) 54, 345
BcoDI GTCTC 1 cut(s) 398
BfaI CTAG 4 cut(s) 96, 428, 579, 602
BfrI CTTAAG 1 cut(s) 400
BfuI GTATCC 1 cut(s) 304
BglII AGATCT 1 cut(s) 793
BisI GCNGC 2 cut(s) 44, 636
BlpI GCTNAGC 1 cut(s) 497
BlsI GCNGC 2 cut(s) 45, 637
Bme1390I CCNGG 4 cut(s) 54, 198, 252, 345
Bme18I GGWCC 3 cut(s) 371, 437, 667
BmeT110I CYCGRG 1 cut(s) 610
BmgT120I GGNCC 6 cut(s) 101, 131, 371, 437, 633, 667
BmiI GGNNCC 4 cut(s) 175, 278, 373, 439
BmrFI CCNGG 4 cut(s) 54, 198, 252, 345
BmrI ACTGGG 1 cut(s) 48
BmsI GCATC 1 cut(s) 811
BmuI ACTGGG 1 cut(s) 48
BplI GAGNNNNNCTC 2 cut(s) 70, 102
BpmI CTGGAG 1 cut(s) 818
Bpu10I CCTNAGC 1 cut(s) 87
Bpu1102I GCTNAGC 1 cut(s) 497
Bpu14I TTCGAA 1 cut(s) 754
BpuMI CCSGG 2 cut(s) 54, 345
Bsa29I ATCGAT 1 cut(s) 446
BsaI GGTCTC 1 cut(s) 398
BsaJI CCNNGG 3 cut(s) 343, 375, 433
Bsc4I CCNNNNNNNGG 6 cut(s) 170, 183, 335, 622, 623, 676
Bse1I ACTGG 2 cut(s) 43, 742
BseBI CCWGG 2 cut(s) 198, 252
BseCI ATCGAT 1 cut(s) 446
BseDI CCNNGG 3 cut(s) 343, 375, 433
BseGI GGATG 1 cut(s) 589
BseLI CCNNNNNNNGG 6 cut(s) 170, 183, 335, 622, 623, 676
BseMII CTCAG 3 cut(s) 101, 701, 742
BseNI ACTGG 2 cut(s) 43, 742
BseSI GKGCMC 1 cut(s) 306
BseXI GCAGC 1 cut(s) 30
BshFI GGCC 6 cut(s) 92, 102, 132, 320, 635, 680
BshNI GGYRCC 1 cut(s) 276
BshVI ATCGAT 1 cut(s) 446
BsiHKAI GWGCWC 1 cut(s) 72
BsiHKCI CYCGRG 1 cut(s) 610
BsiSI CCGG 3 cut(s) 53, 345, 681
BslFI GGGAC 5 cut(s) 69, 253, 312, 357, 423
BslI CCNNNNNNNGG 6 cut(s) 170, 183, 335, 622, 623, 676
BsmAI GTCTC 1 cut(s) 398
BsmFI GGGAC 5 cut(s) 69, 253, 312, 357, 423
BsmI GAATGC 1 cut(s) 750
BsnI GGCC 6 cut(s) 92, 102, 132, 320, 635, 680
Bso31I GGTCTC 1 cut(s) 398
BsoBI CYCGRG 1 cut(s) 610
Bsp119I TTCGAA 1 cut(s) 754
Bsp1286I GDGCHC 3 cut(s) 72, 306, 617
Bsp143I GATC 2 cut(s) 683, 793
Bsp1720I GCTNAGC 1 cut(s) 497
BspACI CCGC 3 cut(s) 330, 617, 636
BspANI GGCC 6 cut(s) 92, 102, 132, 320, 635, 680
BspCNI CTCAG 3 cut(s) 100, 700, 741
BspDI ATCGAT 1 cut(s) 446
BspLI GGNNCC 4 cut(s) 175, 278, 373, 439
BspPI GGATC 1 cut(s) 691
BspT104I TTCGAA 1 cut(s) 754
BspT107I GGYRCC 1 cut(s) 276
BspTI CTTAAG 1 cut(s) 400
BspTNI GGTCTC 1 cut(s) 398
BsrI ACTGG 2 cut(s) 43, 742
BssECI CCNNGG 3 cut(s) 343, 375, 433
BssMI GATC 2 cut(s) 683, 793
BssSI CACGAG 1 cut(s) 71
BssT1I CCWWGG 1 cut(s) 433
Bst2BI CACGAG 1 cut(s) 71
Bst2UI CCWGG 2 cut(s) 198, 252
Bst6I CTCTTC 1 cut(s) 603
BstAFI CTTAAG 1 cut(s) 400
BstBI TTCGAA 1 cut(s) 754
BstC8I GCNNGC 3 cut(s) 517, 617, 722
BstDEI CTNAG 5 cut(s) 87, 470, 497, 687, 728
BstDSI CCRYGG 1 cut(s) 375
BstF5I GGATG 1 cut(s) 589
BstHHI GCGC 1 cut(s) 459
BstKTI GATC 2 cut(s) 686, 796
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 2 cut(s) 683, 793
BstMWI GCNNNNNNNGC 2 cut(s) 49, 512
BstNI CCWGG 2 cut(s) 198, 252
BstSCI CCNGG 4 cut(s) 52, 196, 250, 343
BstSLI GKGCMC 1 cut(s) 306
BstV1I GCAGC 1 cut(s) 30
BstX2I RGATCY 2 cut(s) 683, 793
BstYI RGATCY 2 cut(s) 683, 793
Bsu15I ATCGAT 1 cut(s) 446
BsuI GTATCC 1 cut(s) 304
BsuRI GGCC 6 cut(s) 92, 102, 132, 320, 635, 680
BsuTUI ATCGAT 1 cut(s) 446
BtgI CCRYGG 1 cut(s) 375
BtsCI GGATG 1 cut(s) 589
BtsI GCAGTG 1 cut(s) 508
BtsIMutI CAGTG 2 cut(s) 508, 736
Cac8I GCNNGC 3 cut(s) 517, 617, 722
CfoI GCGC 1 cut(s) 459
Cfr13I GGNCC 6 cut(s) 101, 131, 371, 437, 633, 667
ClaI ATCGAT 1 cut(s) 446
CseI GACGC 1 cut(s) 314
Csp6I GTAC 2 cut(s) 277, 355
CspCI CAANNNNNGTGG 4 cut(s) 235, 270, 509, 544
CviAII CATG 7 cut(s) 273, 307, 316, 322, 493, 554, 713
CviQI GTAC 2 cut(s) 277, 355
DdeI CTNAG 5 cut(s) 87, 470, 497, 687, 728
DpnI GATC 2 cut(s) 685, 795
DpnII GATC 2 cut(s) 683, 793
EaeI YGGCCR 2 cut(s) 318, 678
Eam1104I CTCTTC 1 cut(s) 603
EarI CTCTTC 1 cut(s) 603
Ecl136II GAGCTC 1 cut(s) 70
Eco130I CCWWGG 1 cut(s) 433
Eco147I AGGCCT 1 cut(s) 92
Eco24I GRGCYC 2 cut(s) 72, 617
Eco31I GGTCTC 1 cut(s) 398
Eco32I GATATC 1 cut(s) 194
Eco47I GGWCC 3 cut(s) 371, 437, 667
Eco53kI GAGCTC 1 cut(s) 70
Eco57I CTGAAG 1 cut(s) 767
Eco88I CYCGRG 1 cut(s) 610
EcoICRI GAGCTC 1 cut(s) 70
EcoO109I RGGNCCY 2 cut(s) 371, 437
EcoRI GAATTC 2 cut(s) 23, 759
EcoRII CCWGG 2 cut(s) 196, 250
EcoRV GATATC 1 cut(s) 194
EcoT14I CCWWGG 1 cut(s) 433
EcoT38I GRGCYC 2 cut(s) 72, 617
ErhI CCWWGG 1 cut(s) 433
FaeI CATG 7 cut(s) 276, 310, 319, 325, 496, 557, 716
FaqI GGGAC 5 cut(s) 69, 253, 312, 357, 423
FatI CATG 7 cut(s) 272, 306, 315, 321, 492, 553, 712
FauI CCCGC 2 cut(s) 337, 624
Fnu4HI GCNGC 2 cut(s) 44, 636
FokI GGATG 1 cut(s) 596
FriOI GRGCYC 2 cut(s) 72, 617
Fsp4HI GCNGC 2 cut(s) 44, 636
FspBI CTAG 4 cut(s) 96, 428, 579, 602
GlaI GCGC 1 cut(s) 458
GluI GCNGC 2 cut(s) 44, 636
GsuI CTGGAG 1 cut(s) 818
HaeIII GGCC 6 cut(s) 92, 102, 132, 320, 635, 680
HapII CCGG 3 cut(s) 53, 345, 681
HgaI GACGC 1 cut(s) 314
HhaI GCGC 1 cut(s) 459
Hin1II CATG 7 cut(s) 276, 310, 319, 325, 496, 557, 716
Hin6I GCGC 1 cut(s) 457
HinP1I GCGC 1 cut(s) 457
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HindIII AAGCTT 1 cut(s) 779
HinfI GANTC 1 cut(s) 360
HpaII CCGG 3 cut(s) 53, 345, 681
Hpy166II GTNNAC 2 cut(s) 739, 773
Hpy188I TCNGA 4 cut(s) 575, 690, 765, 786
Hpy188III TCNNGA 5 cut(s) 78, 96, 118, 610, 797
Hpy8I GTNNAC 2 cut(s) 739, 773
HpyAV CCTTC 1 cut(s) 397
HpyCH4IV ACGT 1 cut(s) 353
HpyCH4V TGCA 2 cut(s) 218, 720
HpyF10VI GCNNNNNNNGC 2 cut(s) 49, 512
HpyF3I CTNAG 5 cut(s) 87, 470, 497, 687, 728
HpySE526I ACGT 1 cut(s) 353
Hsp92II CATG 7 cut(s) 276, 310, 319, 325, 496, 557, 716
HspAI GCGC 1 cut(s) 457
KpnI GGTACC 1 cut(s) 280
Kzo9I GATC 2 cut(s) 683, 793
LmnI GCTCC 3 cut(s) 173, 575, 799
Lsp1109I GCAGC 1 cut(s) 30
LweI GCATC 1 cut(s) 811
MaeI CTAG 4 cut(s) 96, 428, 579, 602
MaeII ACGT 1 cut(s) 353
MalI GATC 2 cut(s) 685, 795
MboI GATC 2 cut(s) 683, 793
MboII GAAGA 3 cut(s) 166, 590, 768
MfeI CAATTG 3 cut(s) 135, 219, 647
MflI RGATCY 2 cut(s) 683, 793
MhlI GDGCHC 3 cut(s) 72, 306, 617
MlsI TGGCCA 1 cut(s) 320
MluNI TGGCCA 1 cut(s) 320
MmeI TCCRAC 1 cut(s) 169
MnlI CCTC 6 cut(s) 92, 103, 203, 329, 470, 737
Mox20I TGGCCA 1 cut(s) 320
MscI TGGCCA 1 cut(s) 320
MseI TTAA 3 cut(s) 401, 564, 844
MslI CAYNNNNRTG 3 cut(s) 320, 558, 729
Msp20I TGGCCA 1 cut(s) 320
MspCI CTTAAG 1 cut(s) 400
MspI CCGG 3 cut(s) 53, 345, 681
MspR9I CCNGG 4 cut(s) 54, 198, 252, 345
MunI CAATTG 3 cut(s) 135, 219, 647
Mva1269I GAATGC 1 cut(s) 750
MvaI CCWGG 2 cut(s) 198, 252
MwoI GCNNNNNNNGC 2 cut(s) 49, 512
NciI CCSGG 2 cut(s) 54, 345
NdeII GATC 2 cut(s) 683, 793
NlaIII CATG 7 cut(s) 276, 310, 319, 325, 496, 557, 716
NlaIV GGNNCC 4 cut(s) 175, 278, 373, 439
NmeAIII GCCGAG 1 cut(s) 375
NspV TTCGAA 1 cut(s) 754
OliI CACNNNNGTG 1 cut(s) 729
PaeR7I CTCGAG 1 cut(s) 610
PceI AGGCCT 1 cut(s) 92
PctI GAATGC 1 cut(s) 750
PfeI GAWTC 1 cut(s) 360
PflMI CCANNNNNTGG 3 cut(s) 170, 183, 676
PkrI GCNGC 2 cut(s) 45, 637
PpuMI RGGWCCY 2 cut(s) 371, 437
Psp124BI GAGCTC 1 cut(s) 72
Psp5II RGGWCCY 2 cut(s) 371, 437
Psp6I CCWGG 2 cut(s) 196, 250
PspGI CCWGG 2 cut(s) 196, 250
PspN4I GGNNCC 4 cut(s) 175, 278, 373, 439
PspPI GGNCC 6 cut(s) 101, 131, 371, 437, 633, 667
PspPPI RGGWCCY 2 cut(s) 371, 437
PsuI RGATCY 2 cut(s) 683, 793
RsaI GTAC 2 cut(s) 278, 356
RsaNI GTAC 2 cut(s) 277, 355
RseI CAYNNNNRTG 3 cut(s) 320, 558, 729
SacI GAGCTC 1 cut(s) 72
SaqAI TTAA 3 cut(s) 401, 564, 844
SatI GCNGC 2 cut(s) 44, 636
Sau3AI GATC 2 cut(s) 683, 793
Sau96I GGNCC 6 cut(s) 101, 131, 371, 437, 633, 667
ScrFI CCNGG 4 cut(s) 54, 198, 252, 345
SduI GDGCHC 3 cut(s) 72, 306, 617
SfaNI GCATC 1 cut(s) 811
Sfr274I CTCGAG 1 cut(s) 610
SfuI TTCGAA 1 cut(s) 754
SinI GGWCC 3 cut(s) 371, 437, 667
SlaI CTCGAG 1 cut(s) 610
SmiMI CAYNNNNRTG 3 cut(s) 320, 558, 729
SmlI CTYRAG 2 cut(s) 400, 610
SmoI CTYRAG 2 cut(s) 400, 610
SseBI AGGCCT 1 cut(s) 92
SsiI CCGC 3 cut(s) 330, 617, 636
SspMI CTAG 4 cut(s) 96, 428, 579, 602
SstI GAGCTC 1 cut(s) 72
StuI AGGCCT 1 cut(s) 92
StyD4I CCNGG 4 cut(s) 52, 196, 250, 343
StyI CCWWGG 1 cut(s) 433
TaiI ACGT 1 cut(s) 356
TaqI TCGA 6 cut(s) 3, 15, 77, 446, 611, 754
TaqII GACCGA 1 cut(s) 380
TauI GCSGC 1 cut(s) 638
TfiI GAWTC 1 cut(s) 360
Tru1I TTAA 3 cut(s) 401, 564, 844
Tru9I TTAA 3 cut(s) 401, 564, 844
TscAI CASTG 2 cut(s) 508, 736
TseI GCWGC 1 cut(s) 43
TspDTI ATGAA 2 cut(s) 542, 701
TspRI CASTG 2 cut(s) 508, 736
Van91I CCANNNNNTGG 3 cut(s) 170, 183, 676
Vha464I CTTAAG 1 cut(s) 400
VpaK11BI GGWCC 3 cut(s) 371, 437, 667
XapI RAATTY 2 cut(s) 23, 759
XbaI TCTAGA 1 cut(s) 95
XcmI CCANNNNNNNNNTGG 1 cut(s) 313
XhoI CTCGAG 1 cut(s) 610
XspI CTAG 4 cut(s) 96, 428, 579, 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.