MD16G1158300.v1.1

beta-D-xylosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012799
Physical Location & Seq
Forward (+)
12678643 .. 12684435
5793 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1158300.v1.1.491

Sequence Viewer

Length: 2325 bp
ATGGCATACAATATTGCCAAACTCTCACTTCTTTCTCTTCTGTTCTTATTTTCTTCCCTCTGCAGCATTGCAGTTGTGCATGCTCGTCCACCCTTCGCCTGCGACCCTCGAAACCCGATAACCAGAACGTTGAAGTTCTGCAGGGTGAGGGTGCCAATCCACGTTAGGGTTCAGGACTTGATCGGACGGCTGACATTGCAGGAGAAGATCGGGCTGTTGGTGAACAATGCCATTGCTGTGCCTAGGCTTGGCATTCAGGGGTACGAGTGGTGGTCTGAGGCGCTTCATGGTGTGTCCAACGTGGGGCCTGGAACCAAGTTCGGTACCTTTCTTGGGGCTACTAGCTTCCCTCAAGTCATCACCACCGCTGCTTCCTTCAATGAGTCGCTCTGGGAGGAAATCGGACGGGTTGTGTCTGATGAAGCAAGGGCAATGTACAACGGGGGTGCGGCTGGACTGACATTTTGGAGCCCAAATGTGAACATATTTCGTGACCCGAGGTGGGGAAGGGGGCAAGAGACTCCTGGGGAAGACCCTATCTTGGCTGCCAAGTATGGTGCTAGGTATGTCAAGGGCCTCCAAGGTGATGGTGCTGGGAATCGCCTTAAGGTTGCTGCATGCTGCAAACATTACACCGCCTATGATCTCGATAATTGGAACGGTGTCGATCGGTTCCACTTCAACGCTAGGGTTAGCAAGCAGGACTTGGAAGACACATATAACGTGCCCTTCAGAGCCTGTGTGGTGGATGGGAATGTTGCTAGTGTTATGTGCTCTTACAACCAGGTCAACGGAAAACCTACTTGTGCTGACCCTGAACTCCTCAAAGGCACAATCCGTGGTCAATGGAAACTCAATGGGTATATTGTCTCGGATTGTGATTCAGTTGGTGTTTATTATGATAACCAACATTACACCAAAACACCAGAAGAAGCAGCTGCATACGCAATTAAAGCAGGTTTGGACTTGGATTGTGGGCCATTCCTTGGCATCCACACAGAGGCGGCAGTGAGGTTTGGCCAGGTAAACGAGATCGACATTAACTATGCGTTGGCTAACACAATCACGGTCCAAATGAGATTAGGAATGTTCGATGGCGAACCGTCAGCTCAACGATATGGAAACCTAGGCCTAGCAGATGTGTGCAAACCATCCAGCAATGAGCTAGCCCTTGAAGCTGCCAGACAAGGCATTGTTCTGCTTGAAAACCGTGGGAATTCGCTACCCCTCTCAACCATGCGCCACCGTACTGTGGCTGTAATCGGGCCCAATTCTGATGTTACAGAAACAATGATTGGAAATTATGCAGGTATTGCATGTGGTTACACTACACCACTACAAGGAATTGCGAGGTACACAAGGACCATACACCAAGCTGGGTGCACAGATGTTCATTGCAATGGAAATCAACTAATTGGTGCTGCTGAGGTTGCAGCTAGACAAGCTGATGCAACCGTCTTAGTAATAGGCCTTGACCAATCAATTGAAGCAGAGTTCAGAGACCGGACCGACCTCCTCTTGCCTGGACACCAACAAGAGCTGGTGTCCAGGGTGGCTAGGGCTTCTAGGGGGCCGACCATTCTGGTCATAATGTCAGGCGGCCCTATTGATGTGACGTTTGCAAAGAATGACCCTCGCATTGGAGCGATTATTTGGGTTGGGTATCCAGGCCAGGCTGGAGGAACTGCCATTGCTGATGTTCTATTCGGCACTACAAACCCAAGTGGAAAGCTCCCTATGACATGGTACCCTCAAAATTATGTGGCCAACTTGCCAATGACAGACATGGCCATGAGAGCAGACCCAGCAAGAGGGTATCCTGGTAGGACCTATAGATTTTACAAGGGCCCAGTTGTCTTCCCATTTGGGTTGGGCCTAAGCTACACCAGATTCTCGCACTCCCTAGCCCAGGGGCCTACATTAGTCTCGGTCCCATTCACCAGCCTCGTCGCCTCCAAAAACACAACCATGCTAGGCAACCACGACATCCGAGTGAGCCACACAAATTGTGACTCACTCTCACTCGACGTCCACATTGACATAAAAAACAGCGGAACCATGGATGGGACCCACACTCTATTGGTGTTCGCGACCCCGCCGACAGGAAAATGGGCCCCCAACAAGCAGCTGGTGGGATTTCACAAGGTCCATATCGTGGCCGGATCAGAGAGACGAGTCAGGGTTGGCGTCCAGGTGTGCAAGCACCTCAGCGTCGTTGACGAATTGGGGATCCGGAGAATCCCATTGGGCCAACACAAACTGGAAATTGGTGACCTGCAGCATCATGTTTCTGTTGAAGCAAATTTAGGAGAGATTAAGTTTTAA

Protein Analysis

775

Amino Acids

84.2

Weight (kDa)

8.23

Isoelectric Point (pI)

29.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_3 PF00933 111 - 324 5.3e-35 Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C PF01915 398 - 628 2.6e-52 Glycosyl hydrolase family 3 C-terminal domain
Fn3-like PF14310 694 - 758 8.6e-09 Fibronectin type III-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012799)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49360
fragaria_vesca FvH4_4g19350
malus_domestica MD13G1157400.v1.1 MD16G1158300.v1.1
prunus_persica Prupe.1G123100_v2.0.a1
pyrus_communis pycom16g13320
rosa_chinensis RchiOBHm_Chr4g0424381
rosa_laevigata RLG00000007479
rosa_multiflora Rmu_co8330357.1_g000001 Rmu_sc0003567.1_g000021 Rmu_sc0005995.1_g000003
rosa_roxburghii Rroxscaffold_5G00366330
rosa_rugosa Rorug04G0197100 Rorug04G0197200
rosa_samantha Rh4AG253200 Rh4CG269900 Rh4DG253000
rosa_wichuraiana Rw4G021850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2031
Acc36I ACCTGC 3 cut(s) 947, 1298, 2283
Acc65I GGTACC 2 cut(s) 323, 1746
AccB1I GGYRCC 3 cut(s) 151, 323, 1746
AccB7I CCANNNNNTGG 2 cut(s) 986, 2155
AccII CGCG 1 cut(s) 2090
AccIII TCCGGA 1 cut(s) 2232
AciI CCGC 7 cut(s) 366, 449, 636, 1004, 1599, 2052, 2096
AclI AACGTT 1 cut(s) 128
AclWI GGATC 3 cut(s) 2170, 2224, 2237
AcoI YGGCCR 4 cut(s) 1018, 1764, 1788, 2157
AcsI RAATTY 2 cut(s) 1216, 2302
AcuI CTGAAG 1 cut(s) 715
AcyI GRCGYC 2 cut(s) 2028, 2187
AfaI GTAC 6 cut(s) 263, 325, 437, 1249, 1355, 1748
AflII CTTAAG 1 cut(s) 605
AgsI TTSAA 7 cut(s) 133, 379, 682, 1175, 1205, 1487, 2297
AjuI GAANNNNNNNTTGG 2 cut(s) 1278, 1310
Alw21I GWGCWC 2 cut(s) 776, 1385
Alw26I GTCTC 5 cut(s) 512, 874, 1494, 1930, 2164
Alw44I GTGCAC 1 cut(s) 1381
AlwI GGATC 3 cut(s) 2170, 2224, 2237
AlwNI CAGNNNCTG 1 cut(s) 738
Ama87I CYCGRG 1 cut(s) 496
Aor13HI TCCGGA 1 cut(s) 2232
ApaI GGGCCC 3 cut(s) 1269, 1850, 2116
ApaLI GTGCAC 1 cut(s) 1381
ApoI RAATTY 2 cut(s) 1216, 2302
ArsI GACNNNNNNTTYG 2 cut(s) 447, 479
Asp718I GGTACC 2 cut(s) 323, 1746
AspA2I CCTAGG 2 cut(s) 242, 1126
AspLEI GCGC 2 cut(s) 283, 1242
AsuHPI GGTGA 6 cut(s) 157, 232, 352, 596, 1930, 2282
AsuNHI GCTAGC 1 cut(s) 1165
AvaI CYCGRG 1 cut(s) 496
AvaII GGWCC 7 cut(s) 1069, 1362, 1506, 1827, 1930, 2067, 2146
AvrII CCTAGG 2 cut(s) 242, 1126
BaeGI GKGCMC 5 cut(s) 729, 1269, 1385, 1850, 2116
BalI TGGCCA 3 cut(s) 1020, 1766, 1790
BamHI GGATCC 1 cut(s) 2229
BanI GGYRCC 3 cut(s) 151, 323, 1746
BanII GRGCYC 4 cut(s) 473, 1269, 1850, 2116
BbsI GAAGAC 3 cut(s) 537, 717, 1849
Bbv12I GWGCWC 2 cut(s) 776, 1385
BbvCI CCTCAGC 2 cut(s) 1425, 2207
BccI CCATC 5 cut(s) 581, 743, 1088, 1159, 2057
BceAI ACGGC 1 cut(s) 203
BciVI GTATCC 2 cut(s) 1674, 1827
BcoDI GTCTC 5 cut(s) 512, 874, 1494, 1930, 2164
BfmI CTRYAG 4 cut(s) 61, 139, 1831, 2276
BfoI RGCGCY 1 cut(s) 284
BfrI CTTAAG 1 cut(s) 605
BfuAI ACCTGC 3 cut(s) 947, 1298, 2283
BfuI GTATCC 2 cut(s) 1674, 1827
BlnI CCTAGG 2 cut(s) 242, 1126
Bme18I GGWCC 7 cut(s) 1069, 1362, 1506, 1827, 1930, 2067, 2146
BmeT110I CYCGRG 1 cut(s) 496
BmrI ACTGGG 1 cut(s) 1844
BmsI GCATC 3 cut(s) 999, 1438, 2290
BmtI GCTAGC 1 cut(s) 1169
BmuI ACTGGG 1 cut(s) 1844
BpiI GAAGAC 3 cut(s) 537, 717, 1849
BpmI CTGGAG 1 cut(s) 1698
Bpu10I CCTNAGC 3 cut(s) 1425, 1877, 2207
BpuEI CTTGAG 1 cut(s) 336
BsaHI GRCGYC 2 cut(s) 2028, 2187
BsaI GGTCTC 1 cut(s) 1494
BsaWI WCCGGW 2 cut(s) 1503, 2232
Bse1I ACTGG 2 cut(s) 1850, 2265
Bse3DI GCAATG 8 cut(s) 66, 194, 231, 438, 1165, 1393, 1405, 1689
BseAI TCCGGA 1 cut(s) 2232
BseGI GGATG 5 cut(s) 754, 990, 1151, 1986, 2068
BseMI GCAATG 8 cut(s) 66, 194, 231, 438, 1165, 1393, 1405, 1689
BseMII CTCAG 3 cut(s) 267, 1416, 2221
BseNI ACTGG 2 cut(s) 1850, 2265
BseRI GAGGAG 2 cut(s) 812, 1505
BseSI GKGCMC 5 cut(s) 729, 1269, 1385, 1850, 2116
BseYI CCCAGC 3 cut(s) 593, 1376, 1804
Bsh1236I CGCG 1 cut(s) 2090
Bsh1285I CGRYCG 1 cut(s) 670
BshNI GGYRCC 3 cut(s) 151, 323, 1746
BsiEI CGRYCG 1 cut(s) 670
BsiHKAI GWGCWC 2 cut(s) 776, 1385
BsiHKCI CYCGRG 1 cut(s) 496
BsiSI CCGG 3 cut(s) 1504, 2160, 2233
BslFI GGGAC 2 cut(s) 1916, 2080
BsmAI GTCTC 5 cut(s) 512, 874, 1494, 1930, 2164
BsmBI CGTCTC 1 cut(s) 2164
BsmFI GGGAC 2 cut(s) 1916, 2080
BsmI GAATGC 1 cut(s) 252
Bso31I GGTCTC 1 cut(s) 1494
BsoBI CYCGRG 1 cut(s) 496
Bsp120I GGGCCC 3 cut(s) 1265, 1846, 2112
Bsp1286I GDGCHC 7 cut(s) 473, 729, 776, 1269, 1385, 1850, 2116
Bsp13I TCCGGA 1 cut(s) 2232
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 7 cut(s) 180, 207, 643, 667, 1032, 2162, 2229
Bsp19I CCATGG 1 cut(s) 2058
Bsp68I TCGCGA 1 cut(s) 2090
BspACI CCGC 7 cut(s) 366, 449, 636, 1004, 1599, 2052, 2096
BspCNI CTCAG 3 cut(s) 268, 1417, 2220
BspEI TCCGGA 1 cut(s) 2232
BspFNI CGCG 1 cut(s) 2090
BspMAI CTGCAG 3 cut(s) 65, 143, 2280
BspMI ACCTGC 3 cut(s) 947, 1298, 2283
BspOI GCTAGC 1 cut(s) 1169
BspPI GGATC 3 cut(s) 2170, 2224, 2237
BspT107I GGYRCC 3 cut(s) 151, 323, 1746
BspTI CTTAAG 1 cut(s) 605
BspTNI GGTCTC 1 cut(s) 1494
BsrDI GCAATG 8 cut(s) 66, 194, 231, 438, 1165, 1393, 1405, 1689
BsrGI TGTACA 1 cut(s) 435
BsrI ACTGG 2 cut(s) 1850, 2265
BssMI GATC 7 cut(s) 180, 207, 643, 667, 1032, 2162, 2229
BssNI GRCGYC 2 cut(s) 2028, 2187
BssT1I CCWWGG 5 cut(s) 242, 580, 985, 1126, 2058
Bst4CI ACNGT 7 cut(s) 662, 1069, 1104, 1211, 1247, 1252, 1456
Bst6I CTCTTC 1 cut(s) 42
BstACI GRCGYC 2 cut(s) 2028, 2187
BstAFI CTTAAG 1 cut(s) 605
BstAPI GCANNNNNTGC 1 cut(s) 1313
BstAUI TGTACA 1 cut(s) 435
BstC8I GCNNGC 6 cut(s) 81, 100, 619, 698, 1167, 2201
BstDEI CTNAG 5 cut(s) 276, 1425, 1459, 1877, 2207
BstDSI CCRYGG 3 cut(s) 838, 1210, 2058
BstEII GGTNACC 1 cut(s) 2270
BstENI CCTNNNNNAGG 1 cut(s) 1907
BstF5I GGATG 5 cut(s) 754, 990, 1151, 1986, 2068
BstFNI CGCG 1 cut(s) 2090
BstH2I RGCGCY 1 cut(s) 284
BstHHI GCGC 2 cut(s) 283, 1242
BstKTI GATC 7 cut(s) 183, 210, 646, 670, 1035, 2165, 2232
BstMAI GTCTC 5 cut(s) 512, 874, 1494, 1930, 2164
BstMBI GATC 7 cut(s) 180, 207, 643, 667, 1032, 2162, 2229
BstMCI CGRYCG 1 cut(s) 670
BstMWI GCNNNNNNNGC 9 cut(s) 196, 944, 953, 1175, 1313, 1430, 1442, 1796, 1805
BstNSI RCATGY 3 cut(s) 83, 621, 1320
BstPI GGTNACC 1 cut(s) 2270
BstSFI CTRYAG 4 cut(s) 61, 139, 1831, 2276
BstSLI GKGCMC 5 cut(s) 729, 1269, 1385, 1850, 2116
BstUI CGCG 1 cut(s) 2090
BstV2I GAAGAC 3 cut(s) 537, 717, 1849
BstX2I RGATCY 1 cut(s) 2229
BstXI CCANNNNNNTGG 1 cut(s) 587
BstYI RGATCY 1 cut(s) 2229
BsuI GTATCC 2 cut(s) 1674, 1827
BtgI CCRYGG 3 cut(s) 838, 1210, 2058
BtsCI GGATG 5 cut(s) 754, 990, 1151, 1986, 2068
BtsI GCAGTG 1 cut(s) 1014
BtsIMutI CAGTG 1 cut(s) 1014
BtuMI TCGCGA 1 cut(s) 2090
BveI ACCTGC 3 cut(s) 947, 1298, 2283
Cac8I GCNNGC 6 cut(s) 81, 100, 619, 698, 1167, 2201
CaiI CAGNNNCTG 1 cut(s) 738
CfoI GCGC 2 cut(s) 283, 1242
CpoI CGGWCCG 1 cut(s) 1506
CseI GACGC 2 cut(s) 2176, 2200
CsiI ACCWGGT 1 cut(s) 783
Csp6I GTAC 6 cut(s) 262, 324, 436, 1248, 1354, 1747
CspCI CAANNNNNGTGG 2 cut(s) 1988, 2023
CspI CGGWCCG 1 cut(s) 1506
CviQI GTAC 6 cut(s) 262, 324, 436, 1248, 1354, 1747
DdeI CTNAG 5 cut(s) 276, 1425, 1459, 1877, 2207
DpnI GATC 7 cut(s) 182, 209, 645, 669, 1034, 2164, 2231
DpnII GATC 7 cut(s) 180, 207, 643, 667, 1032, 2162, 2229
EaeI YGGCCR 4 cut(s) 1018, 1764, 1788, 2157
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Eco130I CCWWGG 5 cut(s) 242, 580, 985, 1126, 2058
Eco147I AGGCCT 2 cut(s) 1131, 1470
Eco24I GRGCYC 4 cut(s) 473, 1269, 1850, 2116
Eco31I GGTCTC 1 cut(s) 1494
Eco47I GGWCC 7 cut(s) 1069, 1362, 1506, 1827, 1930, 2067, 2146
Eco57I CTGAAG 1 cut(s) 715
Eco88I CYCGRG 1 cut(s) 496
Eco91I GGTNACC 1 cut(s) 2270
EcoNI CCTNNNNNAGG 1 cut(s) 1907
EcoO109I RGGNCCY 7 cut(s) 305, 574, 1827, 1846, 1913, 2067, 2113
EcoO65I GGTNACC 1 cut(s) 2270
EcoRI GAATTC 1 cut(s) 1216
EcoT14I CCWWGG 5 cut(s) 242, 580, 985, 1126, 2058
EcoT38I GRGCYC 4 cut(s) 473, 1269, 1850, 2116
ErhI CCWWGG 5 cut(s) 242, 580, 985, 1126, 2058
Esp3I CGTCTC 1 cut(s) 2164
FalI AAGNNNNNCTT 2 cut(s) 689, 721
FaqI GGGAC 2 cut(s) 1916, 2080
FauI CCCGC 1 cut(s) 2103
FokI GGATG 5 cut(s) 761, 977, 1138, 1973, 2075
FriOI GRGCYC 4 cut(s) 473, 1269, 1850, 2116
GlaI GCGC 2 cut(s) 282, 1241
GsaI CCCAGC 3 cut(s) 597, 1380, 1808
GsuI CTGGAG 1 cut(s) 1698
HaeII RGCGCY 1 cut(s) 284
HapII CCGG 3 cut(s) 1504, 2160, 2233
HgaI GACGC 2 cut(s) 2176, 2200
HhaI GCGC 2 cut(s) 283, 1242
Hin1I GRCGYC 2 cut(s) 2028, 2187
Hin6I GCGC 2 cut(s) 281, 1240
HinP1I GCGC 2 cut(s) 281, 1240
HincII GTYRAC 2 cut(s) 790, 2218
HindII GTYRAC 2 cut(s) 790, 2218
HinfI GANTC 8 cut(s) 383, 520, 598, 881, 1890, 2012, 2175, 2238
HpaII CCGG 3 cut(s) 1504, 2160, 2233
HphI GGTGA 6 cut(s) 157, 232, 352, 596, 1930, 2282
Hpy166II GTNNAC 9 cut(s) 89, 223, 481, 790, 1027, 1356, 1383, 2032, 2218
Hpy188III TCNNGA 5 cut(s) 173, 491, 647, 2089, 2233
Hpy8I GTNNAC 9 cut(s) 89, 223, 481, 790, 1027, 1356, 1383, 2032, 2218
Hpy99I CGWCG 3 cut(s) 1952, 2030, 2216
HpyAV CCTTC 4 cut(s) 103, 385, 501, 739
HpyCH4III ACNGT 7 cut(s) 662, 1069, 1104, 1211, 1247, 1252, 1456
HpyCH4IV ACGT 6 cut(s) 128, 162, 300, 723, 1616, 2028
HpyF10VI GCNNNNNNNGC 9 cut(s) 196, 944, 953, 1175, 1313, 1430, 1442, 1796, 1805
HpyF3I CTNAG 5 cut(s) 276, 1425, 1459, 1877, 2207
HpySE526I ACGT 6 cut(s) 128, 162, 300, 723, 1616, 2028
Hsp92I GRCGYC 2 cut(s) 2028, 2187
HspAI GCGC 2 cut(s) 281, 1240
KflI GGGWCCC 1 cut(s) 2067
Kpn2I TCCGGA 1 cut(s) 2232
KpnI GGTACC 2 cut(s) 327, 1750
Kzo9I GATC 7 cut(s) 180, 207, 643, 667, 1032, 2162, 2229
LmnI GCTCC 3 cut(s) 468, 1643, 1737
LweI GCATC 3 cut(s) 999, 1438, 2290
MabI ACCWGGT 1 cut(s) 783
MaeII ACGT 6 cut(s) 128, 162, 300, 723, 1616, 2028
MaeIII GTNAC 6 cut(s) 491, 1279, 1322, 1612, 2009, 2270
MalI GATC 7 cut(s) 182, 209, 645, 669, 1034, 2164, 2231
MboI GATC 7 cut(s) 180, 207, 643, 667, 1032, 2162, 2229
MboII GAAGA 7 cut(s) 29, 45, 217, 542, 722, 941, 1849
MfeI CAATTG 1 cut(s) 1482
MflI RGATCY 1 cut(s) 2229
MhlI GDGCHC 7 cut(s) 473, 729, 776, 1269, 1385, 1850, 2116
MlsI TGGCCA 3 cut(s) 1020, 1766, 1790
MluNI TGGCCA 3 cut(s) 1020, 1766, 1790
MlyI GAGTC 4 cut(s) 392, 514, 2006, 2184
MmeI TCCRAC 1 cut(s) 321
Mox20I TGGCCA 3 cut(s) 1020, 1766, 1790
MroI TCCGGA 1 cut(s) 2232
MscI TGGCCA 3 cut(s) 1020, 1766, 1790
MseI TTAA 5 cut(s) 606, 951, 1041, 2316, 2323
MslI CAYNNNNRTG 5 cut(s) 236, 1398, 1790, 1967, 1991
Msp20I TGGCCA 3 cut(s) 1020, 1766, 1790
MspA1I CMGCKG 4 cut(s) 368, 938, 2052, 2128
MspCI CTTAAG 1 cut(s) 605
MspI CCGG 3 cut(s) 1504, 2160, 2233
MunI CAATTG 1 cut(s) 1482
Mva1269I GAATGC 1 cut(s) 252
MvnI CGCG 1 cut(s) 2090
MwoI GCNNNNNNNGC 9 cut(s) 196, 944, 953, 1175, 1313, 1430, 1442, 1796, 1805
NcoI CCATGG 1 cut(s) 2058
NdeII GATC 7 cut(s) 180, 207, 643, 667, 1032, 2162, 2229
NheI GCTAGC 1 cut(s) 1165
NmuCI GTSAC 4 cut(s) 491, 1612, 2009, 2270
NruI TCGCGA 1 cut(s) 2090
NspI RCATGY 3 cut(s) 83, 621, 1320
PaeI GCATGC 2 cut(s) 83, 621
PasI CCCWGGG 1 cut(s) 1909
PceI AGGCCT 2 cut(s) 1131, 1470
PctI GAATGC 1 cut(s) 252
PfeI GAWTC 4 cut(s) 598, 881, 1890, 2238
PflMI CCANNNNNTGG 2 cut(s) 986, 2155
Ple19I CGATCG 1 cut(s) 670
PleI GAGTC 4 cut(s) 391, 514, 2006, 2183
PpsI GAGTC 4 cut(s) 391, 514, 2006, 2183
PpuMI RGGWCCY 2 cut(s) 1827, 2067
Psp1406I AACGTT 1 cut(s) 128
Psp5II RGGWCCY 2 cut(s) 1827, 2067
PspEI GGTNACC 1 cut(s) 2270
PspFI CCCAGC 3 cut(s) 593, 1376, 1804
PspOMI GGGCCC 3 cut(s) 1265, 1846, 2112
PspPPI RGGWCCY 2 cut(s) 1827, 2067
PstI CTGCAG 3 cut(s) 65, 143, 2280
PstNI CAGNNNCTG 1 cut(s) 738
PsuI RGATCY 1 cut(s) 2229
PvuI CGATCG 1 cut(s) 670
PvuII CAGCTG 2 cut(s) 938, 2128
RruI TCGCGA 1 cut(s) 2090
RsaI GTAC 6 cut(s) 263, 325, 437, 1249, 1355, 1748
RsaNI GTAC 6 cut(s) 262, 324, 436, 1248, 1354, 1747
RseI CAYNNNNRTG 5 cut(s) 236, 1398, 1790, 1967, 1991
Rsr2I CGGWCCG 1 cut(s) 1506
RsrII CGGWCCG 1 cut(s) 1506
SaqAI TTAA 5 cut(s) 606, 951, 1041, 2316, 2323
Sau3AI GATC 7 cut(s) 180, 207, 643, 667, 1032, 2162, 2229
SchI GAGTC 4 cut(s) 392, 514, 2006, 2184
SduI GDGCHC 7 cut(s) 473, 729, 776, 1269, 1385, 1850, 2116
SexAI ACCWGGT 1 cut(s) 783
SfaNI GCATC 3 cut(s) 999, 1438, 2290
SfcI CTRYAG 4 cut(s) 61, 139, 1831, 2276
SinI GGWCC 7 cut(s) 1069, 1362, 1506, 1827, 1930, 2067, 2146
SmiMI CAYNNNNRTG 5 cut(s) 236, 1398, 1790, 1967, 1991
SmlI CTYRAG 2 cut(s) 351, 605
SmoI CTYRAG 2 cut(s) 351, 605
SphI GCATGC 2 cut(s) 83, 621
SseBI AGGCCT 2 cut(s) 1131, 1470
SsiI CCGC 7 cut(s) 366, 449, 636, 1004, 1599, 2052, 2096
SspI AATATT 1 cut(s) 13
StuI AGGCCT 2 cut(s) 1131, 1470
StyI CCWWGG 5 cut(s) 242, 580, 985, 1126, 2058
TaaI ACNGT 7 cut(s) 662, 1069, 1104, 1211, 1247, 1252, 1456
TaiI ACGT 6 cut(s) 131, 165, 303, 726, 1619, 2031
TaqI TCGA 6 cut(s) 109, 648, 666, 1035, 1092, 2025
TaqII GACCGA 2 cut(s) 1523, 1918
TatI WGTACW 1 cut(s) 435
TauI GCSGC 3 cut(s) 452, 1007, 1602
TfiI GAWTC 4 cut(s) 598, 881, 1890, 2238
Tru1I TTAA 5 cut(s) 606, 951, 1041, 2316, 2323
Tru9I TTAA 5 cut(s) 606, 951, 1041, 2316, 2323
TscAI CASTG 1 cut(s) 1014
TseFI GTSAC 4 cut(s) 491, 1612, 2009, 2270
Tsp45I GTSAC 4 cut(s) 491, 1612, 2009, 2270
TspDTI ATGAA 3 cut(s) 275, 435, 1382
TspGWI ACGGA 2 cut(s) 807, 827
TspRI CASTG 1 cut(s) 1014
Van91I CCANNNNNTGG 2 cut(s) 986, 2155
Vha464I CTTAAG 1 cut(s) 605
VneI GTGCAC 1 cut(s) 1381
VpaK11BI GGWCC 7 cut(s) 1069, 1362, 1506, 1827, 1930, 2067, 2146
XagI CCTNNNNNAGG 1 cut(s) 1907
XapI RAATTY 2 cut(s) 1216, 2302
XceI RCATGY 3 cut(s) 83, 621, 1320
XcmI CCANNNNNNNNNTGG 1 cut(s) 2125
XmaJI CCTAGG 2 cut(s) 242, 1126
ZraI GACGTC 1 cut(s) 2029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.