MD13G1157400.v1.1

beta-D-xylosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012799
Physical Location & Seq
Forward (+)
12290144 .. 12295004
4861 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1157400.v1.1.491

Sequence Viewer

Length: 2331 bp
ATGGCATACAATATTGCCAAACTTCTCTCACTTTTTTCTCTTCTGTTCTTATTTTCTTCCCTCTGCAACATTGCAGTTGTGCATGCTCGTCCGCCCTTCGCCTGCGACCCTCGAAACCCGATAACGAGAAAGTTGAAGTTCTGCCGGGTGAGGGTGCCAATCCACGATAGGGTTCACGACTTGATCGGACGGCTGACATTGCAGGAGAAGATCAGGCTGTTGGTGAACAATGCCATTGACGTGCCTAGGCTTGGCATCCAGGGCTACGAGTGGTGGTCTGAGGCACTTCATGGTGTGTCCAATGTGGGACCTGGAACCAAGTTTGGTGGGACCTTTCTTGGGGCCACTAGCTTCCCTCAAGTCATCACCACCGCTGCTTCCTTCAACGAGTCGCTCTGGGAGGAAATCGGACGGGTTGTGTCTGATGAAGCAAGAGCAATGTACAACGGGGGCGCGGCTGGACTAACATTTTGGAGCCCAAATGTGAACATATTTCGTGACCCGAGATGGGGTAGAGGGCAAGAGACTCCTGGGGAAGACCCTGTCTTGGCTGCCAAGTATGGTGCTAGGTATGTCAAGGGCCTCCAAGGTGATGGTGTTGGCAATCGCCTTAAGGTTGCTGCATGCTGCAAACATTACACTGCCTATGATCTCGATAACTGGAACGGTGTTGATCGCTTCCACTTCAATGCTAGGGTTAGCAAGCAAGACTTGGAAGACACATATGATGTGCCCTTCAGAGCCTGTGTGGTAGATGGGAATGTTGCTAGTGTTATGTGCTCCTACAACCAGGTCAATGGAAAGCCTACTTGTGCTGACCCTAATCTCCTCAAGGGCACAATCCGCGGCCAATGGAGACTCAATGGGTATATTGTCTCGGATTGTGACTCAGTTGGTGTTTATTATGACGACCAACATTACACCAAGACACCAGAAGAAGCAGCAGCAGACGCAATTAAAGCAGGTTTGGATTTGGATTGTGGGCCGTTCCTCGCCATCCATACGGAGGCGGCCGTGAGGACGGGCCTGGTAAATGAGATTGACATTAACTACGCATTGGCTAATACGATCACGGTCCAAATGAGATTGGGCATGTTCGATGGCGAACCGTCCACTCAGAGATATGGAAACCTAGGCCCAGCAGATGTGTGCAAAATGTCCAGCAATGAGCTAGCCCTTGAAGCTGCCAGGCAAGGCATTGTTCTGCTTGAAAACCGTGGGAATTCGTTGCCCCTCTCAACCACGCGTCATCGTACCGTCGCAGTAATCGGGCCCAATTCTGATGTTACTGAAACAATGATTGGAAATTATGCTGGAGTTGCATGTGGTTACACTACGCCACTACAAGGAATTGCGAGGTACACAAGGACCATACACCAAGCTGGGTGCTCAAATGTTCATTGCAATGGAAACCAACTAATTGGTGCTGCTGAGGCTGCAGCAAGACAGGCTGATGCAACTGTCTTAGTAATAGGCCTTGACCAATCAATCGAAGCAGAGTTCAGAGACCGGACCAACCTCCTCTTGCCCGGACACCAACAGGAGCTGGTGTCCATGGTGGCTAGGGCCTCTAGAGGACCAACCATATTGGTCATAATGTCTGGTAGCCCTATTGATGTTACGTTTGCAAAAAATGACCCGCGCATTGGAGCCATTATTTGGGTTGGGTATCCTGGTCAGGCTGGAGGAACTGCCATTGCCGATGTTTTGTTTGGCACTACGAACCCAAGTGGAAAGCTCCCTATGACATGGTACCCTCAAAACTATGTGGCCAAATTGCCAATGACAGACATGGCCATGAGAGCAAACCCAGCAAGAGGCTACCCTGGCAGGACTTACAGATTCTACAAAGGCCCAGTTGTCTTCCCATTTGGGCTGGGCCTAAGCTACACCAGATTCTCCTACACACTAGCACAGGGGCCCACACTGGTCTCGGTCCCACTCACTAGCCTCGTTGCCGCCAAAAACACAACCATGCTGAACAACAACGGCATCCGAGTCAGCCACATAAACTGCGACTCACTCTCACTCGACATCCATGTTGACGTCAAAAACACCGGAACCATGGACGGGACCCACACTCTTCTGGTGTTCGTGACCCCACCGGCAGAAAAATGGGCCCCCAACAAGCAACTAGTGGGCTTTCACAAGGTCCATATCGTGGCCGGGTCGGAGAGACGGGTTAGGGTTGGCGTCCACGTGTGCAAGCACCTGAGCGTCGTTGACAAATTTGGGATCCGGAGAATCCCATTGGGCGAACACAAACTGGAAATTGGTGACTTGAAGCATCATGTTTCTGTTGAAGCAAATTTAGGAGAGATTAAGTTCTAA

Protein Analysis

777

Amino Acids

84.62

Weight (kDa)

8.55

Isoelectric Point (pI)

29.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_3 PF00933 113 - 327 7.2e-35 Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C PF01915 400 - 630 9.6e-53 Glycosyl hydrolase family 3 C-terminal domain
Fn3-like PF14310 694 - 760 3.5e-09 Fibronectin type III-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012799)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49360
fragaria_vesca FvH4_4g19350
malus_domestica MD13G1157400.v1.1 MD16G1158300.v1.1
prunus_persica Prupe.1G123100_v2.0.a1
pyrus_communis pycom16g13320
rosa_chinensis RchiOBHm_Chr4g0424381
rosa_laevigata RLG00000007479
rosa_multiflora Rmu_co8330357.1_g000001 Rmu_sc0003567.1_g000021 Rmu_sc0005995.1_g000003
rosa_roxburghii Rroxscaffold_5G00366330
rosa_rugosa Rorug04G0197100 Rorug04G0197200
rosa_samantha Rh4AG253200 Rh4CG269900 Rh4DG253000
rosa_wichuraiana Rw4G021850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2049
Acc36I ACCTGC 1 cut(s) 953
Acc65I GGTACC 1 cut(s) 1752
AccB1I GGYRCC 2 cut(s) 154, 1752
AccB7I CCANNNNNTGG 2 cut(s) 1659, 2161
AccII CGCG 4 cut(s) 455, 846, 1246, 1642
AccIII TCCGGA 1 cut(s) 2238
AciI CCGC 8 cut(s) 92, 372, 455, 844, 846, 1010, 1640, 1959
AclWI GGATC 2 cut(s) 2230, 2243
AcoI YGGCCR 5 cut(s) 847, 1011, 1770, 1794, 2163
AcsI RAATTY 3 cut(s) 1222, 2228, 2308
AcuI CTGAAG 1 cut(s) 721
AcvI CACGTG 1 cut(s) 2200
AcyI GRCGYC 2 cut(s) 2046, 2193
AfaI GTAC 4 cut(s) 443, 1255, 1361, 1754
AflII CTTAAG 1 cut(s) 611
AflIII ACRYGT 2 cut(s) 1244, 2199
AgsI TTSAA 7 cut(s) 136, 385, 688, 1181, 1211, 2284, 2303
AhlI ACTAGT 1 cut(s) 2134
AjiI CACGTC 1 cut(s) 241
AjnI CCWGG 8 cut(s) 258, 310, 529, 789, 1026, 1187, 1672, 1825
AjuI GAANNNNNNNTTGG 2 cut(s) 1284, 1316
AluBI AGCT 7 cut(s) 351, 1171, 1184, 1382, 1546, 1738, 1887
AluI AGCT 7 cut(s) 351, 1171, 1184, 1382, 1546, 1738, 1887
Alw21I GWGCWC 2 cut(s) 782, 1391
Alw26I GTCTC 6 cut(s) 518, 850, 880, 1500, 1936, 2170
AlwI GGATC 2 cut(s) 2230, 2243
AlwNI CAGNNNCTG 2 cut(s) 744, 1546
Ama87I CYCGRG 1 cut(s) 502
Aor13HI TCCGGA 1 cut(s) 2238
ApaI GGGCCC 3 cut(s) 1275, 1923, 2122
ApoI RAATTY 3 cut(s) 1222, 2228, 2308
ArsI GACNNNNNNTTYG 2 cut(s) 453, 485
Asp718I GGTACC 1 cut(s) 1752
AspA2I CCTAGG 2 cut(s) 245, 1132
AspLEI GCGC 2 cut(s) 455, 1644
AsuC2I CCSGG 3 cut(s) 146, 1530, 2167
AsuHPI GGTGA 5 cut(s) 160, 235, 358, 602, 2288
AsuNHI GCTAGC 1 cut(s) 1171
AvaI CYCGRG 1 cut(s) 502
AvaII GGWCC 9 cut(s) 308, 330, 1075, 1368, 1512, 1577, 1936, 2073, 2152
AvrII CCTAGG 2 cut(s) 245, 1132
BaeGI GKGCMC 5 cut(s) 735, 839, 1275, 1923, 2122
BalI TGGCCA 2 cut(s) 1772, 1796
BamHI GGATCC 1 cut(s) 2235
BanI GGYRCC 2 cut(s) 154, 1752
BanII GRGCYC 4 cut(s) 479, 1275, 1923, 2122
BbrPI CACGTG 1 cut(s) 2200
BbsI GAAGAC 3 cut(s) 543, 723, 1855
Bbv12I GWGCWC 2 cut(s) 782, 1391
BbvCI CCTCAGC 1 cut(s) 1431
BccI CCATC 5 cut(s) 501, 587, 749, 1004, 1094
BceAI ACGGC 4 cut(s) 206, 970, 998, 2005
BciT130I CCWGG 8 cut(s) 260, 312, 531, 791, 1028, 1189, 1674, 1827
BciVI GTATCC 1 cut(s) 1680
BcnI CCSGG 3 cut(s) 146, 1530, 2167
BcoDI GTCTC 6 cut(s) 518, 850, 880, 1500, 1936, 2170
BcuI ACTAGT 1 cut(s) 2134
BfmI CTRYAG 1 cut(s) 1437
BfrI CTTAAG 1 cut(s) 611
BfuAI ACCTGC 1 cut(s) 953
BfuI GTATCC 1 cut(s) 1680
BlnI CCTAGG 2 cut(s) 245, 1132
Bme18I GGWCC 9 cut(s) 308, 330, 1075, 1368, 1512, 1577, 1936, 2073, 2152
BmeT110I CYCGRG 1 cut(s) 502
BmgBI CACGTC 1 cut(s) 241
BmrI ACTGGG 1 cut(s) 1850
BmsI GCATC 4 cut(s) 264, 1444, 2001, 2296
BmtI GCTAGC 1 cut(s) 1175
BmuI ACTGGG 1 cut(s) 1850
BpiI GAAGAC 3 cut(s) 543, 723, 1855
BpmI CTGGAG 2 cut(s) 1335, 1704
Bpu10I CCTNAGC 3 cut(s) 1431, 1883, 2213
BpuEI CTTGAG 2 cut(s) 342, 815
BpuMI CCSGG 3 cut(s) 146, 1530, 2167
BsaAI YACGTR 1 cut(s) 2200
BsaHI GRCGYC 2 cut(s) 2046, 2193
BsaI GGTCTC 2 cut(s) 1500, 1936
BsaWI WCCGGW 3 cut(s) 1509, 2057, 2238
BsaXI ACNNNNNCTCC 2 cut(s) 1535, 1565
Bse118I RCCGGY 1 cut(s) 2104
Bse1I ACTGG 4 cut(s) 665, 1856, 1932, 2271
Bse3DI GCAATG 7 cut(s) 69, 197, 444, 1171, 1399, 1411, 1695
BseAI TCCGGA 1 cut(s) 2238
BseBI CCWGG 8 cut(s) 260, 312, 531, 791, 1028, 1189, 1674, 1827
BseGI GGATG 4 cut(s) 255, 996, 1992, 2034
BseMI GCAATG 7 cut(s) 69, 197, 444, 1171, 1399, 1411, 1695
BseMII CTCAG 5 cut(s) 270, 903, 1130, 1422, 2204
BseNI ACTGG 4 cut(s) 665, 1856, 1932, 2271
BseRI GAGGAG 2 cut(s) 818, 1511
BseSI GKGCMC 5 cut(s) 735, 839, 1275, 1923, 2122
BseX3I CGGCCG 1 cut(s) 1011
BseYI CCCAGC 4 cut(s) 1138, 1382, 1810, 1876
Bsh1236I CGCG 4 cut(s) 455, 846, 1246, 1642
Bsh1285I CGRYCG 1 cut(s) 1014
BshNI GGYRCC 2 cut(s) 154, 1752
BsiEI CGRYCG 1 cut(s) 1014
BsiHKAI GWGCWC 2 cut(s) 782, 1391
BsiHKCI CYCGRG 1 cut(s) 502
BsiSI CCGG 7 cut(s) 145, 1510, 1530, 2058, 2105, 2166, 2239
BslFI GGGAC 4 cut(s) 321, 343, 1922, 2086
BsmAI GTCTC 6 cut(s) 518, 850, 880, 1500, 1936, 2170
BsmBI CGTCTC 1 cut(s) 2170
BsmFI GGGAC 4 cut(s) 321, 343, 1922, 2086
Bso31I GGTCTC 2 cut(s) 1500, 1936
BsoBI CYCGRG 1 cut(s) 502
Bsp120I GGGCCC 3 cut(s) 1271, 1919, 2118
Bsp1286I GDGCHC 8 cut(s) 479, 735, 782, 839, 1275, 1391, 1923, 2122
Bsp13I TCCGGA 1 cut(s) 2238
Bsp1407I TGTACA 1 cut(s) 441
Bsp143I GATC 6 cut(s) 183, 210, 649, 673, 1068, 2235
Bsp19I CCATGG 2 cut(s) 1554, 2064
BspACI CCGC 8 cut(s) 92, 372, 455, 844, 846, 1010, 1640, 1959
BspCNI CTCAG 5 cut(s) 271, 902, 1129, 1423, 2205
BspEI TCCGGA 1 cut(s) 2238
BspFNI CGCG 4 cut(s) 455, 846, 1246, 1642
BspMAI CTGCAG 1 cut(s) 1441
BspMI ACCTGC 1 cut(s) 953
BspOI GCTAGC 1 cut(s) 1175
BspPI GGATC 2 cut(s) 2230, 2243
BspT107I GGYRCC 2 cut(s) 154, 1752
BspTI CTTAAG 1 cut(s) 611
BspTNI GGTCTC 2 cut(s) 1500, 1936
BsrDI GCAATG 7 cut(s) 69, 197, 444, 1171, 1399, 1411, 1695
BsrFI RCCGGY 1 cut(s) 2104
BsrGI TGTACA 1 cut(s) 441
BsrI ACTGG 4 cut(s) 665, 1856, 1932, 2271
BssAI RCCGGY 1 cut(s) 2104
BssMI GATC 6 cut(s) 183, 210, 649, 673, 1068, 2235
BssNI GRCGYC 2 cut(s) 2046, 2193
BssT1I CCWWGG 5 cut(s) 245, 586, 1132, 1554, 2064
Bst2UI CCWGG 8 cut(s) 260, 312, 531, 791, 1028, 1189, 1674, 1827
Bst4CI ACNGT 6 cut(s) 668, 1075, 1110, 1217, 1258, 1462
Bst6I CTCTTC 2 cut(s) 45, 2088
BstACI GRCGYC 2 cut(s) 2046, 2193
BstAFI CTTAAG 1 cut(s) 611
BstAUI TGTACA 1 cut(s) 441
BstBAI YACGTR 1 cut(s) 2200
BstC8I GCNNGC 6 cut(s) 84, 103, 625, 704, 1173, 2207
BstDEI CTNAG 7 cut(s) 279, 889, 1116, 1431, 1465, 1883, 2213
BstDSI CCRYGG 4 cut(s) 844, 1216, 1554, 2064
BstF5I GGATG 4 cut(s) 255, 996, 1992, 2034
BstFNI CGCG 4 cut(s) 455, 846, 1246, 1642
BstHHI GCGC 2 cut(s) 455, 1644
BstKTI GATC 6 cut(s) 186, 213, 652, 676, 1071, 2238
BstMAI GTCTC 6 cut(s) 518, 850, 880, 1500, 1936, 2170
BstMBI GATC 6 cut(s) 183, 210, 649, 673, 1068, 2235
BstMCI CGRYCG 1 cut(s) 1014
BstNI CCWGG 8 cut(s) 260, 312, 531, 791, 1028, 1189, 1674, 1827
BstNSI RCATGY 4 cut(s) 86, 627, 1096, 1326
BstSFI CTRYAG 1 cut(s) 1437
BstSLI GKGCMC 5 cut(s) 735, 839, 1275, 1923, 2122
BstUI CGCG 4 cut(s) 455, 846, 1246, 1642
BstV2I GAAGAC 3 cut(s) 543, 723, 1855
BstX2I RGATCY 1 cut(s) 2235
BstXI CCANNNNNNTGG 3 cut(s) 593, 797, 1421
BstYI RGATCY 1 cut(s) 2235
BstZI CGGCCG 1 cut(s) 1011
BsuI GTATCC 1 cut(s) 1680
BtgI CCRYGG 4 cut(s) 844, 1216, 1554, 2064
BtrI CACGTC 1 cut(s) 241
BtsCI GGATG 4 cut(s) 255, 996, 1992, 2034
BtsI GCAGTG 1 cut(s) 639
BtsIMutI CAGTG 2 cut(s) 639, 1925
BveI ACCTGC 1 cut(s) 953
Cac8I GCNNGC 6 cut(s) 84, 103, 625, 704, 1173, 2207
CaiI CAGNNNCTG 2 cut(s) 744, 1546
CfoI GCGC 2 cut(s) 455, 1644
Cfr10I RCCGGY 1 cut(s) 2104
Cfr42I CCGCGG 1 cut(s) 847
CseI GACGC 4 cut(s) 959, 1235, 2182, 2206
CsiI ACCWGGT 1 cut(s) 789
Csp6I GTAC 4 cut(s) 442, 1254, 1360, 1753
CspCI CAANNNNNGTGG 2 cut(s) 307, 342
CviQI GTAC 4 cut(s) 442, 1254, 1360, 1753
DdeI CTNAG 7 cut(s) 279, 889, 1116, 1431, 1465, 1883, 2213
DpnI GATC 6 cut(s) 185, 212, 651, 675, 1070, 2237
DpnII GATC 6 cut(s) 183, 210, 649, 673, 1068, 2235
EaeI YGGCCR 5 cut(s) 847, 1011, 1770, 1794, 2163
EagI CGGCCG 1 cut(s) 1011
Eam1104I CTCTTC 2 cut(s) 45, 2088
EarI CTCTTC 2 cut(s) 45, 2088
EciI GGCGGA 1 cut(s) 81
EclXI CGGCCG 1 cut(s) 1011
Eco130I CCWWGG 5 cut(s) 245, 586, 1132, 1554, 2064
Eco147I AGGCCT 1 cut(s) 1476
Eco24I GRGCYC 4 cut(s) 479, 1275, 1923, 2122
Eco31I GGTCTC 2 cut(s) 1500, 1936
Eco47I GGWCC 9 cut(s) 308, 330, 1075, 1368, 1512, 1577, 1936, 2073, 2152
Eco52I CGGCCG 1 cut(s) 1011
Eco57I CTGAAG 1 cut(s) 721
Eco72I CACGTG 1 cut(s) 2200
Eco88I CYCGRG 1 cut(s) 502
EcoO109I RGGNCCY 7 cut(s) 308, 330, 580, 1566, 1919, 2073, 2119
EcoRI GAATTC 1 cut(s) 1222
EcoRII CCWGG 8 cut(s) 258, 310, 529, 789, 1026, 1187, 1672, 1825
EcoT14I CCWWGG 5 cut(s) 245, 586, 1132, 1554, 2064
EcoT38I GRGCYC 4 cut(s) 479, 1275, 1923, 2122
ErhI CCWWGG 5 cut(s) 245, 586, 1132, 1554, 2064
Esp3I CGTCTC 1 cut(s) 2170
FalI AAGNNNNNCTT 2 cut(s) 695, 727
FaqI GGGAC 4 cut(s) 321, 343, 1922, 2086
FauI CCCGC 1 cut(s) 1647
FauNDI CATATG 1 cut(s) 724
FokI GGATG 4 cut(s) 242, 983, 1979, 2021
FriOI GRGCYC 4 cut(s) 479, 1275, 1923, 2122
GlaI GCGC 2 cut(s) 454, 1643
GsaI CCCAGC 4 cut(s) 1142, 1386, 1814, 1880
GsuI CTGGAG 2 cut(s) 1335, 1704
HapII CCGG 7 cut(s) 145, 1510, 1530, 2058, 2105, 2166, 2239
HgaI GACGC 4 cut(s) 959, 1235, 2182, 2206
HhaI GCGC 2 cut(s) 455, 1644
Hin1I GRCGYC 2 cut(s) 2046, 2193
Hin6I GCGC 2 cut(s) 453, 1642
HinP1I GCGC 2 cut(s) 453, 1642
HincII GTYRAC 2 cut(s) 2044, 2224
HindII GTYRAC 2 cut(s) 2044, 2224
HinfI GANTC 9 cut(s) 389, 526, 858, 887, 1842, 1896, 1998, 2018, 2244
HpaII CCGG 7 cut(s) 145, 1510, 1530, 2058, 2105, 2166, 2239
HphI GGTGA 5 cut(s) 160, 235, 358, 602, 2288
Hpy166II GTNNAC 8 cut(s) 175, 226, 487, 1113, 1362, 2044, 2197, 2224
Hpy188III TCNNGA 6 cut(s) 176, 497, 653, 1572, 2095, 2239
Hpy8I GTNNAC 8 cut(s) 175, 226, 487, 1113, 1362, 2044, 2197, 2224
Hpy99I CGWCG 2 cut(s) 1262, 2222
HpyAV CCTTC 3 cut(s) 106, 391, 745
HpyCH4III ACNGT 6 cut(s) 668, 1075, 1110, 1217, 1258, 1462
HpyCH4IV ACGT 4 cut(s) 240, 1622, 2046, 2199
HpyF3I CTNAG 7 cut(s) 279, 889, 1116, 1431, 1465, 1883, 2213
HpySE526I ACGT 4 cut(s) 240, 1622, 2046, 2199
Hsp92I GRCGYC 2 cut(s) 2046, 2193
HspAI GCGC 2 cut(s) 453, 1642
KflI GGGWCCC 1 cut(s) 2073
Kpn2I TCCGGA 1 cut(s) 2238
KpnI GGTACC 1 cut(s) 1756
KspI CCGCGG 1 cut(s) 847
Kzo9I GATC 6 cut(s) 183, 210, 649, 673, 1068, 2235
LmnI GCTCC 5 cut(s) 474, 785, 1543, 1649, 1743
LweI GCATC 4 cut(s) 264, 1444, 2001, 2296
MabI ACCWGGT 1 cut(s) 789
MaeII ACGT 4 cut(s) 240, 1622, 2046, 2199
MaeIII GTNAC 7 cut(s) 497, 884, 1285, 1328, 1618, 2095, 2276
MalI GATC 6 cut(s) 185, 212, 651, 675, 1070, 2237
MboI GATC 6 cut(s) 183, 210, 649, 673, 1068, 2235
MboII GAAGA 8 cut(s) 32, 48, 220, 548, 728, 947, 1855, 2075
MflI RGATCY 1 cut(s) 2235
MhlI GDGCHC 8 cut(s) 479, 735, 782, 839, 1275, 1391, 1923, 2122
MlsI TGGCCA 2 cut(s) 1772, 1796
MluI ACGCGT 1 cut(s) 1244
MluNI TGGCCA 2 cut(s) 1772, 1796
MlyI GAGTC 6 cut(s) 398, 520, 852, 881, 2007, 2012
MmeI TCCRAC 1 cut(s) 2151
Mox20I TGGCCA 2 cut(s) 1772, 1796
MroI TCCGGA 1 cut(s) 2238
MscI TGGCCA 2 cut(s) 1772, 1796
MseI TTAA 4 cut(s) 612, 957, 1047, 2322
MslI CAYNNNNRTG 5 cut(s) 239, 687, 1404, 1796, 1973
Msp20I TGGCCA 2 cut(s) 1772, 1796
MspA1I CMGCKG 2 cut(s) 374, 846
MspCI CTTAAG 1 cut(s) 611
MspI CCGG 7 cut(s) 145, 1510, 1530, 2058, 2105, 2166, 2239
MvaI CCWGG 8 cut(s) 260, 312, 531, 791, 1028, 1189, 1674, 1827
MvnI CGCG 4 cut(s) 455, 846, 1246, 1642
NciI CCSGG 3 cut(s) 146, 1530, 2167
NcoI CCATGG 2 cut(s) 1554, 2064
NdeI CATATG 1 cut(s) 724
NdeII GATC 6 cut(s) 183, 210, 649, 673, 1068, 2235
NheI GCTAGC 1 cut(s) 1171
NmuCI GTSAC 4 cut(s) 497, 884, 2095, 2276
NspI RCATGY 4 cut(s) 86, 627, 1096, 1326
PaeI GCATGC 2 cut(s) 86, 627
PceI AGGCCT 1 cut(s) 1476
PfeI GAWTC 3 cut(s) 1842, 1896, 2244
PflFI GACNNNGTC 1 cut(s) 542
PflMI CCANNNNNTGG 2 cut(s) 1659, 2161
PleI GAGTC 6 cut(s) 397, 520, 852, 881, 2006, 2012
PmaCI CACGTG 1 cut(s) 2200
PmlI CACGTG 1 cut(s) 2200
PpsI GAGTC 6 cut(s) 397, 520, 852, 881, 2006, 2012
Ppu21I YACGTR 1 cut(s) 2200
PpuMI RGGWCCY 3 cut(s) 308, 330, 2073
Psp5II RGGWCCY 3 cut(s) 308, 330, 2073
Psp6I CCWGG 8 cut(s) 258, 310, 529, 789, 1026, 1187, 1672, 1825
PspCI CACGTG 1 cut(s) 2200
PspFI CCCAGC 4 cut(s) 1138, 1382, 1810, 1876
PspGI CCWGG 8 cut(s) 258, 310, 529, 789, 1026, 1187, 1672, 1825
PspOMI GGGCCC 3 cut(s) 1271, 1919, 2118
PspPPI RGGWCCY 3 cut(s) 308, 330, 2073
PstI CTGCAG 1 cut(s) 1441
PstNI CAGNNNCTG 2 cut(s) 744, 1546
PsuI RGATCY 1 cut(s) 2235
PsyI GACNNNGTC 1 cut(s) 542
RsaI GTAC 4 cut(s) 443, 1255, 1361, 1754
RsaNI GTAC 4 cut(s) 442, 1254, 1360, 1753
RseI CAYNNNNRTG 5 cut(s) 239, 687, 1404, 1796, 1973
SacII CCGCGG 1 cut(s) 847
SaqAI TTAA 4 cut(s) 612, 957, 1047, 2322
Sau3AI GATC 6 cut(s) 183, 210, 649, 673, 1068, 2235
SchI GAGTC 6 cut(s) 398, 520, 852, 881, 2007, 2012
SduI GDGCHC 8 cut(s) 479, 735, 782, 839, 1275, 1391, 1923, 2122
SexAI ACCWGGT 1 cut(s) 789
SfaNI GCATC 4 cut(s) 264, 1444, 2001, 2296
SfcI CTRYAG 1 cut(s) 1437
Sfr303I CCGCGG 1 cut(s) 847
SgrBI CCGCGG 1 cut(s) 847
SinI GGWCC 9 cut(s) 308, 330, 1075, 1368, 1512, 1577, 1936, 2073, 2152
SmiMI CAYNNNNRTG 5 cut(s) 239, 687, 1404, 1796, 1973
SmlI CTYRAG 3 cut(s) 357, 611, 830
SmoI CTYRAG 3 cut(s) 357, 611, 830
SpeI ACTAGT 1 cut(s) 2134
SphI GCATGC 2 cut(s) 86, 627
SseBI AGGCCT 1 cut(s) 1476
SsiI CCGC 8 cut(s) 92, 372, 455, 844, 846, 1010, 1640, 1959
SspI AATATT 1 cut(s) 13
StuI AGGCCT 1 cut(s) 1476
StyI CCWWGG 5 cut(s) 245, 586, 1132, 1554, 2064
TaaI ACNGT 6 cut(s) 668, 1075, 1110, 1217, 1258, 1462
TaiI ACGT 4 cut(s) 243, 1625, 2049, 2202
TaqI TCGA 5 cut(s) 112, 654, 1098, 1491, 2031
TaqII GACCGA 1 cut(s) 1924
TatI WGTACW 1 cut(s) 441
TauI GCSGC 4 cut(s) 458, 849, 1013, 1961
TfiI GAWTC 3 cut(s) 1842, 1896, 2244
Tru1I TTAA 4 cut(s) 612, 957, 1047, 2322
Tru9I TTAA 4 cut(s) 612, 957, 1047, 2322
TscAI CASTG 2 cut(s) 646, 1932
TseFI GTSAC 4 cut(s) 497, 884, 2095, 2276
Tsp45I GTSAC 4 cut(s) 497, 884, 2095, 2276
TspDTI ATGAA 3 cut(s) 278, 441, 1388
TspGWI ACGGA 1 cut(s) 1019
TspRI CASTG 2 cut(s) 646, 1932
Tth111I GACNNNGTC 1 cut(s) 542
Van91I CCANNNNNTGG 2 cut(s) 1659, 2161
Vha464I CTTAAG 1 cut(s) 611
VpaK11BI GGWCC 9 cut(s) 308, 330, 1075, 1368, 1512, 1577, 1936, 2073, 2152
XapI RAATTY 3 cut(s) 1222, 2228, 2308
XbaI TCTAGA 1 cut(s) 1571
XceI RCATGY 4 cut(s) 86, 627, 1096, 1326
XmaJI CCTAGG 2 cut(s) 245, 1132
ZraI GACGTC 1 cut(s) 2047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.