Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0012799

Physical Location & Seq

Genomic Coordinates Reverse (-)

56791108 .. 56795057

Gene Structure 3950 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG253200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2331 bp

ATGGCTTCTGGTTACAATAATAAGCTTTCCCTTCTTGCTCTTGTTCTCTGTGTATCAGCATTGTTGCTCTGCAGCACGTTTAATCTGGTACATGCGCGTCCACCCTTTGCATGTGACCCCCGGAACCCGTTAACGAGAGGGTTCAAGTTCTGCAGGACGAGGGTGCCGGTCCACATGAGGGTTCAGGACCTGATCGGACGGCTGACATTGCAGGAGAAGATTAGGCTGCTGGTGAACAATGCCATAGCTGTGCCGAGGCTCGGCATTCAGGGGTACGAGTGGTGGTCGGAGGCGCTTCACGGCGTCTCTAATGTGGGCCCCGGGACTAAGTTTGGCGGGGCATTTCCGGGCGCCACTAGCTTCCCTCAAGTCATCACCACCGCCGCTTCCTTCAATCAATCACTGTGGCAAGAAATCGGTCAGGTGGTGTCTGATGAAGCAAGAGCAATGTACAATGGCGGCCAGGCTGGTTTGACATATTGGAGCCCTAACGTGAACATATTCCGTGACCCACGATGGGGAAGAGGACAAGAGACTCCTGGGGAAGACCCTGTTTTGGCTGCTAAGTATGCTGCTAGCTATGTCAAGGGTCTCCAAGGTGATGGAGCTGGAAACCGGCTTAAGGTTGCGGCATGTTGTAAGCATTACACGGCCTATGATCTTGACAATTGGAATGGCGTCGATCGCTTCCATTTCAATGCCAGGGTTAGCAAGCAGGACTTGGCAGACACATATGACGTGCCGTTTAGAGGCTGTGTGTTAGAAGGAAAGGTTGCTAGTGTCATGTGCTCATACAACCAAGTCAATGGAAAGCCTACTTGTGCTGACCCTGATCTCCTAAAGAACACAATCCGTGGCCAATGGAAACTCAATGGGTACATAGTCTCCGACTGTGACTCGGTGGGAGTCTTGTATGATGAGCAACATTACACCAAGACACCGGAAGAAGCAGCGGCGGACTCGATCAAAGCGGGTTTGGATCTGGACTGTGGGCCGTTTCTCGCAATCCATACGGAGGGCGCTATAAAGTCCGGCCTGCTACAAGAGATCGACGTGAACTATGCGCTAGCAAACACTCTTACGGTCCAAATGAGACTCGGGATGTTCGATGGCGAACCATCGGCCCAACAATATGGAAACTTGGGCCCAAGAGATGTTTGCACCCCGGCCCACCAACAGTTGGCACTTGAGGCCGCTAGGCAAGGGATTGTCCTGCTTCAAAACAATCGGCATTCGCTGCCTCTCTCGACTGTGCGTCATCGTACCATAGCGGTAATTGGGCCCAACTCGGATGTTACCGTCACGATGATAGGAAATTATGCTGGTGTTGCATGTGGCTACACAACTCCCCTGCAAGGAATTGGGAGGTACACAAAGACCATTCATCAACAAGGGTGCACCGATGTTGCTTGCACTGGTAACCAACTATTTGGGGCAGCTGAGGCTGCAGCCCGGCAGGCTGATGCTACCGTCTTAGTGATGGGCCTCGACCAATCTATTGAAGCGGAATTCCGGGACAGGACTGGGCTGCTCTTGCCGGGACATCAACAGGAGCTCGTGTCGAGAGTGGCTCGGGCCTCTAGAGGCCCAACCATATTGGTCTTGATGTCTGGTGGCCCAATTGATGTGTCGTTTGCGAAGAATGACCCAAAAATTGGAGCCATAATTTGGGTTGGATATCCTGGTCAAGCAGGAGGAACTGCAATTGCTGATGTTCTGTTTGGGACTACCAATCCAGGTGGGAAGCTACCGATGACATGGTACCCCCAAGACTATGTATCCAAAGTGCCCATGACAAACATGGCCATGCGTCCCGCCAGAGGCTACCCCGGTAGGACGTACCGGTTCTACAAAGGTCCCGTGGTATTTCCCTTCGGTCTCGGCTTAAGCTACACCACCTTTGCCCACTCACTAGCCCAAGTTCCCACATCGGTTTCGGTGCGCCTCACAAGTCTAAGTGCCACCACAAACTCAACCATGCTAAGCAGTGCTGTGAGAGTCAGCCACACAAATTGCAATTCGCTCTCACTTCCTGTTCATGTTGATGTTAAAAACACCGGAGCTAGGGATGGAACTCACACTCTTCTAGTGTTCTCGAGCCCGCCTTTGGGGAAATGGGCCGCGAACAAGCAATTAGTGGGCTTTCACAAGGTCCATATTCTGGCCGGGTCTCAAAAACGGGTCAAGGTTGATGTTCATGTTTGCAAGCACCTCAGTGTTGTTGACCAGTTTGGGATTCGAAGAATTCCGATTGGTGAACATAAGCTTCAGATTGGAGATCTGGAGCATCACATCTCCATTGAAGCCAATTTAGGAGATATTAGATCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005576 GO:0005618 GO:0005623 GO:0007275 GO:0008150 GO:0009505 GO:0009791 GO:0010154 GO:0010214 GO:0016787 GO:0016798 GO:0022414 GO:0030312 GO:0032501 GO:0032502 GO:0044464 GO:0046556 GO:0048046 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Fn3-like Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

776

Amino Acids

83.98

Weight (kDa)

8.87

Isoelectric Point (pI)

32.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	115 - 330	2.7e-34	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	403 - 631	1.5e-51	Glycosyl hydrolase family 3 C-terminal domain
Fn3-like	PF14310	697 - 760	3e-08	Fibronectin type III-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012799)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49360
fragaria_vesca	FvH4_4g19350
malus_domestica	MD13G1157400.v1.1 MD16G1158300.v1.1
prunus_persica	Prupe.1G123100_v2.0.a1
pyrus_communis	pycom16g13320
rosa_chinensis	RchiOBHm_Chr4g0424381
rosa_laevigata	RLG00000007479
rosa_multiflora	Rmu_co8330357.1_g000001 Rmu_sc0003567.1_g000021 Rmu_sc0005995.1_g000003
rosa_roxburghii	Rroxscaffold_5G00366330
rosa_rugosa	Rorug04G0197100 Rorug04G0197200
rosa_samantha	Rh4AG253200 Rh4CG269900 Rh4DG253000
rosa_wichuraiana	Rw4G021850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1761
AccB1I	GGYRCC	3 cut(s)	163, 350, 1761
AccB7I	CCANNNNNTGG	4 cut(s)	1180, 1655, 1668, 2161
AccII	CGCG	2 cut(s)	97, 2123
AclWI	GGATC	1 cut(s)	987
AcoI	YGGCCR	4 cut(s)	460, 856, 1803, 2163
AcsI	RAATTY	2 cut(s)	1508, 2244
AcuI	CTGAAG	1 cut(s)	2252
AcyI	GRCGYC	3 cut(s)	303, 351, 678
AfaI	GTAC	8 cut(s)	90, 275, 452, 878, 1264, 1370, 1763, 1841
AflII	CTTAAG	2 cut(s)	620, 1885
AgeI	ACCGGT	1 cut(s)	1842
AgsI	TTSAA	6 cut(s)	145, 394, 697, 1220, 1502, 2303
AhdI	GACNNNNNGTC	1 cut(s)	1254
AjiI	CACGTC	2 cut(s)	739, 1054
AjnI	CCWGG	5 cut(s)	462, 538, 701, 1681, 1735
AleI	CACNNNNGTG	1 cut(s)	2214
Alw21I	GWGCWC	3 cut(s)	791, 1400, 1557
Alw26I	GTCTC	7 cut(s)	310, 527, 596, 889, 1087, 1883, 2175
Alw44I	GTGCAC	1 cut(s)	1396
AlwI	GGATC	1 cut(s)	987
AlwNI	CAGNNNCTG	1 cut(s)	190
Ama87I	CYCGRG	4 cut(s)	320, 1097, 1572, 2095
ApaI	GGGCCC	3 cut(s)	320, 1148, 1284
ApaLI	GTGCAC	1 cut(s)	1396
ApoI	RAATTY	2 cut(s)	1508, 2244
AsiGI	ACCGGT	1 cut(s)	1842
Asp700I	GAANNNNTTC	1 cut(s)	500
Asp718I	GGTACC	1 cut(s)	1761
AspLEI	GCGC	6 cut(s)	97, 295, 353, 1022, 1066, 1944
AsuHPI	GGTGA	4 cut(s)	244, 367, 611, 2267
AsuII	TTCGAA	1 cut(s)	2239
AsuNHI	GCTAGC	2 cut(s)	575, 1066
AvaI	CYCGRG	4 cut(s)	320, 1097, 1572, 2095
AvaII	GGWCC	5 cut(s)	169, 187, 1084, 1856, 2152
BaeGI	GKGCMC	5 cut(s)	320, 1148, 1284, 1400, 1791
BalI	TGGCCA	2 cut(s)	858, 1805
BanI	GGYRCC	3 cut(s)	163, 350, 1761
BanII	GRGCYC	6 cut(s)	320, 488, 1148, 1284, 1557, 2102
BauI	CACGAG	1 cut(s)	1556
BbsI	GAAGAC	1 cut(s)	552
Bbv12I	GWGCWC	3 cut(s)	791, 1400, 1557
BbvCI	CCTCAGC	1 cut(s)	1440
BccI	CCATC	6 cut(s)	510, 596, 1103, 1126, 1474, 2063
BceAI	ACGGC	5 cut(s)	215, 316, 666, 727, 979
BciT130I	CCWGG	5 cut(s)	464, 540, 703, 1683, 1737
BciVI	GTATCC	1 cut(s)	1789
BcoDI	GTCTC	7 cut(s)	310, 527, 596, 889, 1087, 1883, 2175
BfaI	CTAG	9 cut(s)	357, 576, 777, 1067, 1197, 1581, 1913, 2064, 2087
BfmI	CTRYAG	3 cut(s)	70, 151, 1446
BfoI	RGCGCY	3 cut(s)	296, 354, 1023
BfrI	CTTAAG	2 cut(s)	620, 1885
BfuI	GTATCC	1 cut(s)	1789
BglI	GCCNNNNNGGC	1 cut(s)	1457
BglII	AGATCT	2 cut(s)	2278, 2324
BlpI	GCTNAGC	1 cut(s)	1982
Bme18I	GGWCC	5 cut(s)	169, 187, 1084, 1856, 2152
BmeRI	GACNNNNNGTC	1 cut(s)	1254
BmeT110I	CYCGRG	4 cut(s)	320, 1097, 1572, 2095
BmgBI	CACGTC	2 cut(s)	739, 1054
BmrI	ACTGGG	1 cut(s)	1533
BmsI	GCATC	2 cut(s)	1453, 2296
BmtI	GCTAGC	2 cut(s)	579, 1070
BmuI	ACTGGG	1 cut(s)	1533
BpiI	GAAGAC	1 cut(s)	552
BplI	GAGNNNNNCTC	2 cut(s)	1555, 1587
BpmI	CTGGAG	1 cut(s)	2303
Bpu10I	CCTNAGC	1 cut(s)	1440
Bpu1102I	GCTNAGC	1 cut(s)	1982
Bpu14I	TTCGAA	1 cut(s)	2239
BpuEI	CTTGAG	2 cut(s)	351, 1208
BsaHI	GRCGYC	3 cut(s)	303, 351, 678
BsaI	GGTCTC	3 cut(s)	596, 1883, 2175
BsaWI	WCCGGW	3 cut(s)	940, 1842, 2057
BsaXI	ACNNNNNCTCC	4 cut(s)	869, 899, 1544, 1574
Bse118I	RCCGGY	3 cut(s)	166, 615, 1842
Bse1I	ACTGG	3 cut(s)	1420, 1528, 2227
Bse3DI	GCAATG	2 cut(s)	206, 453
BseBI	CCWGG	5 cut(s)	464, 540, 703, 1683, 1737
BseGI	GGATG	3 cut(s)	1107, 1297, 2074
BseMI	GCAATG	2 cut(s)	206, 453
BseMII	CTCAG	2 cut(s)	1431, 2227
BseNI	ACTGG	3 cut(s)	1420, 1528, 2227
BseSI	GKGCMC	5 cut(s)	320, 1148, 1284, 1400, 1791
Bsh1236I	CGCG	2 cut(s)	97, 2123
Bsh1285I	CGRYCG	1 cut(s)	685
BshNI	GGYRCC	3 cut(s)	163, 350, 1761
BshTI	ACCGGT	1 cut(s)	1842
BsiEI	CGRYCG	1 cut(s)	685
BsiHKAI	GWGCWC	3 cut(s)	791, 1400, 1557
BsiHKCI	CYCGRG	4 cut(s)	320, 1097, 1572, 2095
BslFI	GGGAC	6 cut(s)	337, 1529, 1554, 1738, 1797, 1842
BsmAI	GTCTC	7 cut(s)	310, 527, 596, 889, 1087, 1883, 2175
BsmBI	CGTCTC	1 cut(s)	310
BsmFI	GGGAC	6 cut(s)	337, 1529, 1554, 1738, 1797, 1842
BsmI	GAATGC	2 cut(s)	264, 1231
Bso31I	GGTCTC	3 cut(s)	596, 1883, 2175
BsoBI	CYCGRG	4 cut(s)	320, 1097, 1572, 2095
Bsp119I	TTCGAA	1 cut(s)	2239
Bsp120I	GGGCCC	3 cut(s)	316, 1144, 1280
Bsp1286I	GDGCHC	9 cut(s)	320, 488, 791, 1148, 1284, 1400, 1557, 1791, 2102
Bsp1407I	TGTACA	1 cut(s)	450
Bsp143I	GATC	9 cut(s)	192, 658, 682, 832, 963, 979, 1047, 2278, 2324
Bsp1720I	GCTNAGC	1 cut(s)	1982
BspCNI	CTCAG	2 cut(s)	1432, 2226
BspFNI	CGCG	2 cut(s)	97, 2123
BspMAI	CTGCAG	3 cut(s)	74, 155, 1450
BspOI	GCTAGC	2 cut(s)	579, 1070
BspPI	GGATC	1 cut(s)	987
BspT104I	TTCGAA	1 cut(s)	2239
BspT107I	GGYRCC	3 cut(s)	163, 350, 1761
BspTI	CTTAAG	2 cut(s)	620, 1885
BspTNI	GGTCTC	3 cut(s)	596, 1883, 2175
BsrDI	GCAATG	2 cut(s)	206, 453
BsrFI	RCCGGY	3 cut(s)	166, 615, 1842
BsrGI	TGTACA	1 cut(s)	450
BsrI	ACTGG	3 cut(s)	1420, 1528, 2227
BssAI	RCCGGY	3 cut(s)	166, 615, 1842
BssMI	GATC	9 cut(s)	192, 658, 682, 832, 963, 979, 1047, 2278, 2324
BssNI	GRCGYC	3 cut(s)	303, 351, 678
BssSI	CACGAG	1 cut(s)	1556
BssT1I	CCWWGG	1 cut(s)	595
Bst2BI	CACGAG	1 cut(s)	1556
Bst2UI	CCWGG	5 cut(s)	464, 540, 703, 1683, 1737
Bst4CI	ACNGT	8 cut(s)	405, 893, 989, 1084, 1179, 1252, 1300, 1471
Bst6I	CTCTTC	2 cut(s)	517, 2088
BstACI	GRCGYC	3 cut(s)	303, 351, 678
BstAFI	CTTAAG	2 cut(s)	620, 1885
BstAPI	GCANNNNNTGC	1 cut(s)	1237
BstAUI	TGTACA	1 cut(s)	450
BstBI	TTCGAA	1 cut(s)	2239
BstC8I	GCNNGC	8 cut(s)	577, 713, 1037, 1068, 1411, 1458, 2102, 2207
BstDEI	CTNAG	7 cut(s)	327, 564, 1440, 1474, 1955, 1982, 2213
BstDSI	CCRYGG	2 cut(s)	853, 1860
BstEII	GGTNACC	1 cut(s)	1418
BstF5I	GGATG	3 cut(s)	1107, 1297, 2074
BstFNI	CGCG	2 cut(s)	97, 2123
BstH2I	RGCGCY	3 cut(s)	296, 354, 1023
BstHHI	GCGC	6 cut(s)	97, 295, 353, 1022, 1066, 1944
BstKTI	GATC	9 cut(s)	195, 661, 685, 835, 966, 982, 1050, 2281, 2327
BstMAI	GTCTC	7 cut(s)	310, 527, 596, 889, 1087, 1883, 2175
BstMBI	GATC	9 cut(s)	192, 658, 682, 832, 963, 979, 1047, 2278, 2324
BstMCI	CGRYCG	1 cut(s)	685
BstNI	CCWGG	5 cut(s)	464, 540, 703, 1683, 1737
BstNSI	RCATGY	4 cut(s)	95, 114, 636, 1335
BstPI	GGTNACC	1 cut(s)	1418
BstSFI	CTRYAG	3 cut(s)	70, 151, 1446
BstSLI	GKGCMC	5 cut(s)	320, 1148, 1284, 1400, 1791
BstUI	CGCG	2 cut(s)	97, 2123
BstV2I	GAAGAC	1 cut(s)	552
BstX2I	RGATCY	3 cut(s)	979, 2278, 2324
BstXI	CCANNNNNNTGG	4 cut(s)	602, 806, 1133, 1430
BstYI	RGATCY	3 cut(s)	979, 2278, 2324
BsuI	GTATCC	1 cut(s)	1789
BtgI	CCRYGG	2 cut(s)	853, 1860
BtrI	CACGTC	2 cut(s)	739, 1054
BtsCI	GGATG	3 cut(s)	1107, 1297, 2074
BtsI	GCAGTG	1 cut(s)	1993
BtsIMutI	CAGTG	4 cut(s)	401, 1413, 1993, 2221
Cac8I	GCNNGC	8 cut(s)	577, 713, 1037, 1068, 1411, 1458, 2102, 2207
CaiI	CAGNNNCTG	1 cut(s)	190
CfoI	GCGC	6 cut(s)	97, 295, 353, 1022, 1066, 1944
Cfr10I	RCCGGY	3 cut(s)	166, 615, 1842
Cfr9I	CCCGGG	1 cut(s)	320
CseI	GACGC	5 cut(s)	86, 292, 667, 1244, 1799
Csp6I	GTAC	8 cut(s)	89, 274, 451, 877, 1263, 1369, 1762, 1840
CspAI	ACCGGT	1 cut(s)	1842
CspCI	CAANNNNNGTGG	6 cut(s)	386, 421, 1720, 1755, 1994, 2029
CviQI	GTAC	8 cut(s)	89, 274, 451, 877, 1263, 1369, 1762, 1840
DdeI	CTNAG	7 cut(s)	327, 564, 1440, 1474, 1955, 1982, 2213
DinI	GGCGCC	1 cut(s)	352
DpnI	GATC	9 cut(s)	194, 660, 684, 834, 965, 981, 1049, 2280, 2326
DpnII	GATC	9 cut(s)	192, 658, 682, 832, 963, 979, 1047, 2278, 2324
DriI	GACNNNNNGTC	1 cut(s)	1254
EaeI	YGGCCR	4 cut(s)	460, 856, 1803, 2163
Eam1104I	CTCTTC	2 cut(s)	517, 2088
Eam1105I	GACNNNNNGTC	1 cut(s)	1254
EarI	CTCTTC	2 cut(s)	517, 2088
EciI	GGCGGA	1 cut(s)	971
Ecl136II	GAGCTC	1 cut(s)	1555
Eco130I	CCWWGG	1 cut(s)	595
Eco24I	GRGCYC	6 cut(s)	320, 488, 1148, 1284, 1557, 2102
Eco31I	GGTCTC	3 cut(s)	596, 1883, 2175
Eco32I	GATATC	1 cut(s)	1679
Eco47I	GGWCC	5 cut(s)	169, 187, 1084, 1856, 2152
Eco53kI	GAGCTC	1 cut(s)	1555
Eco57I	CTGAAG	1 cut(s)	2252
Eco88I	CYCGRG	4 cut(s)	320, 1097, 1572, 2095
Eco91I	GGTNACC	1 cut(s)	1418
EcoICRI	GAGCTC	1 cut(s)	1555
EcoO109I	RGGNCCY	3 cut(s)	187, 317, 1856
EcoO65I	GGTNACC	1 cut(s)	1418
EcoRI	GAATTC	2 cut(s)	1508, 2244
EcoRII	CCWGG	5 cut(s)	462, 538, 701, 1681, 1735
EcoRV	GATATC	1 cut(s)	1679
EcoT14I	CCWWGG	1 cut(s)	595
EcoT38I	GRGCYC	6 cut(s)	320, 488, 1148, 1284, 1557, 2102
EgeI	GGCGCC	1 cut(s)	352
EheI	GGCGCC	1 cut(s)	352
ErhI	CCWWGG	1 cut(s)	595
Esp3I	CGTCTC	1 cut(s)	310
FalI	AAGNNNNNCTT	2 cut(s)	704, 736
FaqI	GGGAC	6 cut(s)	337, 1529, 1554, 1738, 1797, 1842
FauI	CCCGC	4 cut(s)	329, 964, 1822, 2109
FauNDI	CATATG	1 cut(s)	733
FokI	GGATG	3 cut(s)	1114, 1304, 2081
FriOI	GRGCYC	6 cut(s)	320, 488, 1148, 1284, 1557, 2102
FspBI	CTAG	9 cut(s)	357, 576, 777, 1067, 1197, 1581, 1913, 2064, 2087
GlaI	GCGC	6 cut(s)	96, 294, 352, 1021, 1065, 1943
GsuI	CTGGAG	1 cut(s)	2303
HaeII	RGCGCY	3 cut(s)	296, 354, 1023
HgaI	GACGC	5 cut(s)	86, 292, 667, 1244, 1799
HhaI	GCGC	6 cut(s)	97, 295, 353, 1022, 1066, 1944
Hin1I	GRCGYC	3 cut(s)	303, 351, 678
Hin6I	GCGC	6 cut(s)	95, 293, 351, 1020, 1064, 1942
HinP1I	GCGC	6 cut(s)	95, 293, 351, 1020, 1064, 1942
HincII	GTYRAC	2 cut(s)	132, 2224
HindII	GTYRAC	2 cut(s)	132, 2224
HindIII	AAGCTT	2 cut(s)	23, 2264
HinfI	GANTC	7 cut(s)	535, 896, 906, 959, 1095, 1998, 2236
HpaI	GTTAAC	1 cut(s)	132
HphI	GGTGA	4 cut(s)	244, 367, 611, 2267
Hpy188I	TCNGA	7 cut(s)	197, 289, 433, 889, 1291, 2250, 2271
Hpy99I	CGWCG	2 cut(s)	683, 1055
HpyAV	CCTTC	4 cut(s)	41, 400, 758, 1882
HpyCH4III	ACNGT	8 cut(s)	405, 893, 989, 1084, 1179, 1252, 1300, 1471
HpyCH4IV	ACGT	5 cut(s)	77, 492, 738, 1053, 1838
HpyF3I	CTNAG	7 cut(s)	327, 564, 1440, 1474, 1955, 1982, 2213
HpySE526I	ACGT	5 cut(s)	77, 492, 738, 1053, 1838
Hsp92I	GRCGYC	3 cut(s)	303, 351, 678
HspAI	GCGC	6 cut(s)	95, 293, 351, 1020, 1064, 1942
KasI	GGCGCC	1 cut(s)	350
KpnI	GGTACC	1 cut(s)	1765
KspAI	GTTAAC	1 cut(s)	132
Kzo9I	GATC	9 cut(s)	192, 658, 682, 832, 963, 979, 1047, 2278, 2324
LmnI	GCTCC	6 cut(s)	483, 605, 1552, 1658, 2060, 2284
LweI	GCATC	2 cut(s)	1453, 2296
MaeI	CTAG	9 cut(s)	357, 576, 777, 1067, 1197, 1581, 1913, 2064, 2087
MaeII	ACGT	5 cut(s)	77, 492, 738, 1053, 1838
MaeIII	GTNAC	7 cut(s)	11, 113, 506, 893, 1294, 1300, 1418
MalI	GATC	9 cut(s)	194, 660, 684, 834, 965, 981, 1049, 2280, 2326
MboI	GATC	9 cut(s)	192, 658, 682, 832, 963, 979, 1047, 2278, 2324
MboII	GAAGA	7 cut(s)	229, 534, 557, 956, 1651, 2075, 2253
MfeI	CAATTG	3 cut(s)	667, 1620, 1704
MflI	RGATCY	3 cut(s)	979, 2278, 2324
MhlI	GDGCHC	9 cut(s)	320, 488, 791, 1148, 1284, 1400, 1557, 1791, 2102
MlsI	TGGCCA	2 cut(s)	858, 1805
MluNI	TGGCCA	2 cut(s)	858, 1805
Mly113I	GGCGCC	1 cut(s)	351
MlyI	GAGTC	6 cut(s)	529, 890, 915, 953, 1089, 2007
MmeI	TCCRAC	3 cut(s)	267, 912, 1654
Mox20I	TGGCCA	2 cut(s)	858, 1805
MroXI	GAANNNNTTC	1 cut(s)	500
MscI	TGGCCA	2 cut(s)	858, 1805
MseI	TTAA	6 cut(s)	81, 131, 621, 1886, 2049, 2329
MslI	CAYNNNNRTG	5 cut(s)	248, 696, 1805, 2043, 2214
Msp20I	TGGCCA	2 cut(s)	858, 1805
MspA1I	CMGCKG	2 cut(s)	953, 1439
MspCI	CTTAAG	2 cut(s)	620, 1885
MunI	CAATTG	3 cut(s)	667, 1620, 1704
Mva1269I	GAATGC	2 cut(s)	264, 1231
MvaI	CCWGG	5 cut(s)	464, 540, 703, 1683, 1737
MvnI	CGCG	2 cut(s)	97, 2123
NarI	GGCGCC	1 cut(s)	351
NdeI	CATATG	1 cut(s)	733
NdeII	GATC	9 cut(s)	192, 658, 682, 832, 963, 979, 1047, 2278, 2324
NheI	GCTAGC	2 cut(s)	575, 1066
NmeAIII	GCCGAG	3 cut(s)	240, 279, 1860
NmuCI	GTSAC	4 cut(s)	113, 506, 893, 1300
NspI	RCATGY	4 cut(s)	95, 114, 636, 1335
NspV	TTCGAA	1 cut(s)	2239
OliI	CACNNNNGTG	1 cut(s)	2214
PaeR7I	CTCGAG	1 cut(s)	2095
PcsI	WCGNNNNNNNCGW	1 cut(s)	1104
PctI	GAATGC	2 cut(s)	264, 1231
PdmI	GAANNNNTTC	1 cut(s)	500
PfeI	GAWTC	1 cut(s)	2236
PflMI	CCANNNNNTGG	4 cut(s)	1180, 1655, 1668, 2161
PfoI	TCCNGGA	1 cut(s)	1512
PinAI	ACCGGT	1 cut(s)	1842
Ple19I	CGATCG	1 cut(s)	685
PleI	GAGTC	6 cut(s)	529, 890, 914, 953, 1089, 2006
PluTI	GGCGCC	1 cut(s)	354
PpsI	GAGTC	6 cut(s)	529, 890, 914, 953, 1089, 2006
PpuMI	RGGWCCY	2 cut(s)	187, 1856
Psp124BI	GAGCTC	1 cut(s)	1557
Psp5II	RGGWCCY	2 cut(s)	187, 1856
Psp6I	CCWGG	5 cut(s)	462, 538, 701, 1681, 1735
PspEI	GGTNACC	1 cut(s)	1418
PspGI	CCWGG	5 cut(s)	462, 538, 701, 1681, 1735
PspOMI	GGGCCC	3 cut(s)	316, 1144, 1280
PspPPI	RGGWCCY	2 cut(s)	187, 1856
PstI	CTGCAG	3 cut(s)	74, 155, 1450
PstNI	CAGNNNCTG	1 cut(s)	190
PsuI	RGATCY	3 cut(s)	979, 2278, 2324
PvuI	CGATCG	1 cut(s)	685
PvuII	CAGCTG	1 cut(s)	1439
RsaI	GTAC	8 cut(s)	90, 275, 452, 878, 1264, 1370, 1763, 1841
RsaNI	GTAC	8 cut(s)	89, 274, 451, 877, 1263, 1369, 1762, 1840
RseI	CAYNNNNRTG	5 cut(s)	248, 696, 1805, 2043, 2214
SacI	GAGCTC	1 cut(s)	1557
SaqAI	TTAA	6 cut(s)	81, 131, 621, 1886, 2049, 2329
Sau3AI	GATC	9 cut(s)	192, 658, 682, 832, 963, 979, 1047, 2278, 2324
SchI	GAGTC	6 cut(s)	529, 890, 915, 953, 1089, 2007
SduI	GDGCHC	9 cut(s)	320, 488, 791, 1148, 1284, 1400, 1557, 1791, 2102
SfaNI	GCATC	2 cut(s)	1453, 2296
SfcI	CTRYAG	3 cut(s)	70, 151, 1446
SfoI	GGCGCC	1 cut(s)	352
Sfr274I	CTCGAG	1 cut(s)	2095
SfuI	TTCGAA	1 cut(s)	2239
SinI	GGWCC	5 cut(s)	169, 187, 1084, 1856, 2152
SlaI	CTCGAG	1 cut(s)	2095
SmaI	CCCGGG	1 cut(s)	322
SmiMI	CAYNNNNRTG	5 cut(s)	248, 696, 1805, 2043, 2214
SmlI	CTYRAG	5 cut(s)	366, 620, 1187, 1885, 2095
SmoI	CTYRAG	5 cut(s)	366, 620, 1187, 1885, 2095
SspDI	GGCGCC	1 cut(s)	350
SspMI	CTAG	9 cut(s)	357, 576, 777, 1067, 1197, 1581, 1913, 2064, 2087
SstI	GAGCTC	1 cut(s)	1557
StyI	CCWWGG	1 cut(s)	595
TaaI	ACNGT	8 cut(s)	405, 893, 989, 1084, 1179, 1252, 1300, 1471
TaiI	ACGT	5 cut(s)	80, 495, 741, 1056, 1841
TaqI	TCGA	9 cut(s)	681, 962, 1050, 1107, 1247, 1488, 1562, 2096, 2239
TaqII	GACCGA	2 cut(s)	407, 1865
TatI	WGTACW	1 cut(s)	450
TauI	GCSGC	6 cut(s)	386, 462, 632, 956, 1196, 2123
TfiI	GAWTC	1 cut(s)	2236
Tru1I	TTAA	6 cut(s)	81, 131, 621, 1886, 2049, 2329
Tru9I	TTAA	6 cut(s)	81, 131, 621, 1886, 2049, 2329
TscAI	CASTG	4 cut(s)	408, 1420, 1993, 2221
TseFI	GTSAC	4 cut(s)	113, 506, 893, 1300
Tsp45I	GTSAC	4 cut(s)	113, 506, 893, 1300
TspDTI	ATGAA	4 cut(s)	450, 1373, 2027, 2186
TspGWI	ACGGA	3 cut(s)	494, 842, 1028
TspMI	CCCGGG	1 cut(s)	320
TspRI	CASTG	4 cut(s)	408, 1420, 1993, 2221
Van91I	CCANNNNNTGG	4 cut(s)	1180, 1655, 1668, 2161
Vha464I	CTTAAG	2 cut(s)	620, 1885
VneI	GTGCAC	1 cut(s)	1396
VpaK11BI	GGWCC	5 cut(s)	169, 187, 1084, 1856, 2152
XapI	RAATTY	2 cut(s)	1508, 2244
XbaI	TCTAGA	1 cut(s)	1580
XceI	RCATGY	4 cut(s)	95, 114, 636, 1335
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1798
XhoI	CTCGAG	1 cut(s)	2095
XmaI	CCCGGG	1 cut(s)	320
XmnI	GAANNNNTTC	1 cut(s)	500
XspI	CTAG	9 cut(s)	357, 576, 777, 1067, 1197, 1581, 1913, 2064, 2087

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.