FvH4_4g20690

F-box-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0012734
Physical Location & Seq
Reverse (-)
23820553 .. 23821638
1086 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g20690.t1

Sequence Viewer

Length: 1086 bp
ATGAATTCACAAAGACATGGCTTTTTCCCGGACGAAGTCGTCCTTCAAATTCTTTCTAGGCTGCCGATTAAGTCACTTTTCAGGAGCAAAACTGTGTGCAAGCTTTGGTGTAGATTGGTTTCTGATAAGTATTTCATTCAGTTGTACAATGAATTGGCTGTGAAGAACTCTATGTTGTTGGTTGAGGTCTCCGAGTCGTCTGGTTTGAAATCCAGTTTGATTTGTGTTGATGATCAGAAGGGTGTTTGTGAATTTGACTTGGGCTTCTTGAAGGACAGGGTTAAGGTTAGAGCATCGTGTAATGGTTTGTTGTGTTGCTCTAGTATTCCTGATAAGGGTGTTTATTATGTTTGTAATCCTATGACTCGTGAGTTTAAGCTGCTTCCGAAGAGTAGGGAGAGGAATGTGACTCGGTTTTATCCTGATGGTGAGGCCACTTTGGTTGGATTGGCTTGCAATATGGCTTCACAAAAGTTTAATGTGGTCTTAGCTGGCTATCACCGCACTTTTGGTCATAGGCCGGATGGGACTTTTAAGTGTATGATATATGATTCTGAGACGAACAAATGGAGGAAGTTTATTACTTTTCAGGATGATCAGTTTAGCCATATGAATAAGAACCAGGTTGTGTTTGTGAATGGCATGCTTCATTGGTTGACGGGTGGTGGTTGTTCTTGTATACTTGTGCTTGATTTGGAGTATGATATGTGGAGGAAGATGTCGTTGCCTGATGAATTGAACTGTGGAATGGGGAATCGGTTTTACTTGTTGGAGTTGGATGGTTGCTTGTCTGTGATTCAGATTTCAGATACTTGGATGAAGATTTGGGTGTTGAAAGATTATGTGAGAGAGGAATGGCGTTTGGTTGATACTGTGAGTATGAGATGTATCAAAGGACTTGTTCCGGGGATTTTCCCCATATGTCAGACTGGTGAATATGTTATCTTAGCAACCCATAAGCAGATATTGGTGTTTCATCGCAAAAGTCGTGTGTGGAAAGAGATGTACTCAGTGAAGAACAGCTCTACACTCCCACTGTGGTACTCAGCTCATGCATTTCAGAGCACGATTTCTTCTTGCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_3

Protein Analysis

362

Amino Acids

41.67

Weight (kDa)

8.81

Isoelectric Point (pI)

35.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 10 - 45 1.3e-08 F-box domain
F-box-like PF12937 10 - 44 7.9e-09 F-box-like
FBA_1 PF07734 80 - 336 7.9e-16 F-box associated beta propeller domain
FBA_3 PF08268 84 - 316 1.4e-12 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 94 - 302 2e-12 F-box protein At3g26010-like, beta-propeller
Beta-prop_AT5G49610-like PF23635 96 - 341 7.1e-08 F-box protein AT5G49610-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012734)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49610
fragaria_vesca FvH4_4g20690
malus_domestica MD13G1148700.v1.1 MD16G1149000.v1.1
prunus_persica Prupe.1G112700_v2.0.a1
pyrus_communis pycom13g12800 pycom16g12720
rosa_chinensis RchiOBHm_Chr4g0426111
rosa_laevigata RLG00000007318
rosa_multiflora Rmu_co8375493.1_g000001 Rmu_ssc0000281.1_g000004
rosa_roxburghii Rroxscaffold_5G00368100
rosa_rugosa Rorug04G0209100
rosa_samantha Rh4AG265600 Rh4BG271700 Rh4CG284600 Rh4DG267800
rosa_wichuraiana Rw4G022950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 38
AccI GTMKAC 1 cut(s) 679
AciI CCGC 1 cut(s) 502
AcsI RAATTY 3 cut(s) 4, 48, 251
AfaI GTAC 3 cut(s) 146, 1007, 1043
AfiI CCNNNNNNNGG 1 cut(s) 335
AgsI TTSAA 5 cut(s) 47, 208, 271, 739, 835
AjnI CCWGG 1 cut(s) 621
AluBI AGCT 5 cut(s) 103, 379, 491, 1023, 1049
AluI AGCT 5 cut(s) 103, 379, 491, 1023, 1049
Alw21I GWGCWC 1 cut(s) 1067
Alw26I GTCTC 2 cut(s) 193, 551
AoxI GGCC 2 cut(s) 432, 518
ApeKI GCWGC 2 cut(s) 61, 379
ApoI RAATTY 3 cut(s) 4, 48, 251
AsuC2I CCSGG 2 cut(s) 29, 906
AsuHPI GGTGA 3 cut(s) 440, 491, 944
BauI CACGAG 1 cut(s) 366
Bbv12I GWGCWC 1 cut(s) 1067
BbvI GCAGC 2 cut(s) 48, 366
BccI CCATC 3 cut(s) 419, 518, 773
BciT130I CCWGG 1 cut(s) 623
BclI TGATCA 2 cut(s) 232, 595
BcnI CCSGG 2 cut(s) 29, 906
BcoDI GTCTC 2 cut(s) 193, 551
BfaI CTAG 2 cut(s) 57, 321
BisI GCNGC 2 cut(s) 62, 380
BlsI GCNGC 2 cut(s) 63, 381
Bme1390I CCNGG 3 cut(s) 29, 623, 906
BmrFI CCNGG 3 cut(s) 29, 623, 906
BmsI GCATC 1 cut(s) 302
BplI GAGNNNNNCTC 2 cut(s) 992, 1024
BpuMI CCSGG 2 cut(s) 29, 906
BsaI GGTCTC 1 cut(s) 193
BsaJI CCNNGG 1 cut(s) 905
Bsc4I CCNNNNNNNGG 1 cut(s) 335
Bse1I ACTGG 2 cut(s) 213, 934
BseBI CCWGG 1 cut(s) 623
BseDI CCNNGG 1 cut(s) 905
BseGI GGATG 4 cut(s) 529, 598, 784, 822
BseLI CCNNNNNNNGG 1 cut(s) 335
BseMII CTCAG 3 cut(s) 546, 1023, 1059
BseNI ACTGG 2 cut(s) 213, 934
BseXI GCAGC 2 cut(s) 48, 366
BshFI GGCC 2 cut(s) 434, 520
BsiHKAI GWGCWC 1 cut(s) 1067
BsiSI CCGG 3 cut(s) 29, 521, 905
BslFI GGGAC 1 cut(s) 541
BslI CCNNNNNNNGG 1 cut(s) 335
BsmAI GTCTC 2 cut(s) 193, 551
BsmBI CGTCTC 1 cut(s) 551
BsmFI GGGAC 1 cut(s) 541
BsnI GGCC 2 cut(s) 434, 520
Bso31I GGTCTC 1 cut(s) 193
Bsp1286I GDGCHC 1 cut(s) 1067
Bsp1407I TGTACA 1 cut(s) 144
Bsp143I GATC 2 cut(s) 232, 595
BspACI CCGC 1 cut(s) 502
BspANI GGCC 2 cut(s) 434, 520
BspCNI CTCAG 3 cut(s) 547, 1022, 1058
BspTNI GGTCTC 1 cut(s) 193
BsrGI TGTACA 1 cut(s) 144
BsrI ACTGG 2 cut(s) 213, 934
BssECI CCNNGG 1 cut(s) 905
BssMI GATC 2 cut(s) 232, 595
BssNAI GTATAC 1 cut(s) 680
BssSI CACGAG 1 cut(s) 366
Bst1107I GTATAC 1 cut(s) 680
Bst2BI CACGAG 1 cut(s) 366
Bst2UI CCWGG 1 cut(s) 623
Bst4CI ACNGT 4 cut(s) 94, 743, 874, 1038
Bst6I CTCTTC 1 cut(s) 383
BstAUI TGTACA 1 cut(s) 144
BstC8I GCNNGC 4 cut(s) 101, 454, 493, 644
BstDEI CTNAG 5 cut(s) 487, 555, 946, 1009, 1045
BstF5I GGATG 4 cut(s) 529, 598, 784, 822
BstKTI GATC 2 cut(s) 235, 598
BstMAI GTCTC 2 cut(s) 193, 551
BstMBI GATC 2 cut(s) 232, 595
BstMWI GCNNNNNNNGC 1 cut(s) 501
BstNI CCWGG 1 cut(s) 623
BstNSI RCATGY 1 cut(s) 646
BstSCI CCNGG 3 cut(s) 27, 621, 904
BstV1I GCAGC 2 cut(s) 48, 366
BstZ17I GTATAC 1 cut(s) 680
BsuRI GGCC 2 cut(s) 434, 520
BtgZI GCGATG 1 cut(s) 962
BtsCI GGATG 4 cut(s) 529, 598, 784, 822
BtsIMutI CAGTG 2 cut(s) 1017, 1034
Cac8I GCNNGC 4 cut(s) 101, 454, 493, 644
CsiI ACCWGGT 1 cut(s) 621
Csp6I GTAC 3 cut(s) 145, 1006, 1042
CspCI CAANNNNNGTGG 2 cut(s) 424, 459
CviAII CATG 3 cut(s) 17, 643, 1052
CviQI GTAC 3 cut(s) 145, 1006, 1042
DdeI CTNAG 5 cut(s) 487, 555, 946, 1009, 1045
DpnI GATC 2 cut(s) 234, 597
DpnII GATC 2 cut(s) 232, 595
DrdI GACNNNNNNGTC 1 cut(s) 38
DseDI GACNNNNNNGTC 1 cut(s) 38
Eam1104I CTCTTC 1 cut(s) 383
EarI CTCTTC 1 cut(s) 383
Eco31I GGTCTC 1 cut(s) 193
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 1 cut(s) 621
EcoT22I ATGCAT 1 cut(s) 1057
Esp3I CGTCTC 1 cut(s) 551
FaeI CATG 3 cut(s) 20, 646, 1055
FalI AAGNNNNNCTT 2 cut(s) 27, 59
FaqI GGGAC 1 cut(s) 541
FatI CATG 3 cut(s) 16, 642, 1051
FauNDI CATATG 2 cut(s) 609, 920
FbaI TGATCA 2 cut(s) 232, 595
FblI GTMKAC 1 cut(s) 679
Fnu4HI GCNGC 2 cut(s) 62, 380
FokI GGATG 4 cut(s) 536, 605, 791, 829
Fsp4HI GCNGC 2 cut(s) 62, 380
FspBI CTAG 2 cut(s) 57, 321
GluI GCNGC 2 cut(s) 62, 380
HaeIII GGCC 2 cut(s) 434, 520
HapII CCGG 3 cut(s) 29, 521, 905
Hin1II CATG 3 cut(s) 20, 646, 1055
HincII GTYRAC 1 cut(s) 657
HindII GTYRAC 1 cut(s) 657
HindIII AAGCTT 1 cut(s) 101
HinfI GANTC 6 cut(s) 194, 364, 409, 551, 754, 796
HpaII CCGG 3 cut(s) 29, 521, 905
HphI GGTGA 3 cut(s) 440, 491, 944
Hpy166II GTNNAC 2 cut(s) 657, 680
Hpy188I TCNGA 9 cut(s) 124, 193, 237, 387, 556, 801, 808, 927, 1062
Hpy188III TCNNGA 6 cut(s) 82, 268, 329, 368, 422, 590
Hpy8I GTNNAC 2 cut(s) 657, 680
HpyAV CCTTC 3 cut(s) 53, 232, 265
HpyCH4III ACNGT 4 cut(s) 94, 743, 874, 1038
HpyCH4V TGCA 3 cut(s) 99, 456, 1055
HpyF10VI GCNNNNNNNGC 1 cut(s) 501
HpyF3I CTNAG 5 cut(s) 487, 555, 946, 1009, 1045
Hsp92II CATG 3 cut(s) 20, 646, 1055
Ksp22I TGATCA 2 cut(s) 232, 595
Kzo9I GATC 2 cut(s) 232, 595
LmnI GCTCC 1 cut(s) 84
Lsp1109I GCAGC 2 cut(s) 48, 366
LweI GCATC 1 cut(s) 302
MabI ACCWGGT 1 cut(s) 621
MaeI CTAG 2 cut(s) 57, 321
MaeIII GTNAC 2 cut(s) 72, 406
MalI GATC 2 cut(s) 234, 597
MboI GATC 2 cut(s) 232, 595
MboII GAAGA 6 cut(s) 175, 400, 727, 832, 1027, 1065
MhlI GDGCHC 1 cut(s) 1067
MluCI AATT 5 cut(s) 4, 48, 152, 251, 734
MlyI GAGTC 3 cut(s) 203, 358, 403
MmeI TCCRAC 3 cut(s) 424, 750, 756
MnlI CCTC 6 cut(s) 178, 393, 424, 564, 705, 844
Mph1103I ATGCAT 1 cut(s) 1057
MseI TTAA 5 cut(s) 69, 282, 375, 477, 534
MspI CCGG 3 cut(s) 29, 521, 905
MspR9I CCNGG 3 cut(s) 29, 623, 906
MvaI CCWGG 1 cut(s) 623
MwoI GCNNNNNNNGC 1 cut(s) 501
NciI CCSGG 2 cut(s) 29, 906
NdeI CATATG 2 cut(s) 609, 920
NdeII GATC 2 cut(s) 232, 595
NlaIII CATG 3 cut(s) 20, 646, 1055
NmuCI GTSAC 2 cut(s) 72, 406
NsiI ATGCAT 1 cut(s) 1057
NspI RCATGY 1 cut(s) 646
PaeI GCATGC 1 cut(s) 646
PcsI WCGNNNNNNNCGW 1 cut(s) 985
PfeI GAWTC 3 cut(s) 551, 754, 796
PflFI GACNNNGTC 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 27
PkrI GCNGC 2 cut(s) 63, 381
PleI GAGTC 3 cut(s) 202, 358, 403
PpsI GAGTC 3 cut(s) 202, 358, 403
Psp6I CCWGG 1 cut(s) 621
PspGI CCWGG 1 cut(s) 621
PsyI GACNNNGTC 1 cut(s) 35
RsaI GTAC 3 cut(s) 146, 1007, 1043
RsaNI GTAC 3 cut(s) 145, 1006, 1042
SaqAI TTAA 5 cut(s) 69, 282, 375, 477, 534
SatI GCNGC 2 cut(s) 62, 380
Sau3AI GATC 2 cut(s) 232, 595
SchI GAGTC 3 cut(s) 203, 358, 403
ScrFI CCNGG 3 cut(s) 29, 623, 906
SduI GDGCHC 1 cut(s) 1067
SetI ASST 8 cut(s) 105, 189, 288, 381, 493, 627, 1025, 1051
SexAI ACCWGGT 1 cut(s) 621
SfaNI GCATC 1 cut(s) 302
SphI GCATGC 1 cut(s) 646
Sse9I AATT 5 cut(s) 4, 48, 152, 251, 734
SsiI CCGC 1 cut(s) 502
SspMI CTAG 2 cut(s) 57, 321
StyD4I CCNGG 3 cut(s) 27, 621, 904
TaaI ACNGT 4 cut(s) 94, 743, 874, 1038
TasI AATT 5 cut(s) 4, 48, 152, 251, 734
TatI WGTACW 2 cut(s) 144, 1005
TfiI GAWTC 3 cut(s) 551, 754, 796
Tru1I TTAA 5 cut(s) 69, 282, 375, 477, 534
Tru9I TTAA 5 cut(s) 69, 282, 375, 477, 534
TscAI CASTG 2 cut(s) 1017, 1041
TseFI GTSAC 2 cut(s) 72, 406
TseI GCWGC 2 cut(s) 61, 379
Tsp45I GTSAC 2 cut(s) 72, 406
TspDTI ATGAA 8 cut(s) 17, 124, 165, 626, 638, 747, 833, 965
TspRI CASTG 2 cut(s) 1017, 1041
Tth111I GACNNNGTC 1 cut(s) 35
XapI RAATTY 3 cut(s) 4, 48, 251
XceI RCATGY 1 cut(s) 646
XmiI GTMKAC 1 cut(s) 679
XspI CTAG 2 cut(s) 57, 321
Zsp2I ATGCAT 1 cut(s) 1057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.